Method for Extracting Ginseng Essential Oil by Combining Deep Eutectic Solvent-Supercritical Technology with Column Chromatography

Abstract

The present invention relates to the technical field of drug extraction, and particularly to a method for extracting ginseng essential oil by combining eutectic solvent-supercritical technology with column chromatography. The method comprises the following steps: (1) pulverize ginseng root whiskers and add them to a supercritical extraction kettle, and carry out supercritical extraction under the combined action of liquid CO2 and an entrainer, so as to obtain an extract; the entrainer includes absolute ethanol and a eutectic solvent; (2) concentrate the extract until there is no ethanol, dilute it with water, adsorb it on macroporous resin for column chromatography, and successively carry out water washing, elution with 2%-5% ethanol, and elution with 10%-20% ethanol, collect the 10%-20% ethanol eluate, and obtain the product after drying. The present invention has the advantages of high yield, high content of panaxynol, short extraction period, and no environmental pollution.

Description

Technical Field

[0001] The present invention relates to the technical field of drug extraction, and specifically relates to a method for extracting ginseng essential oil by combining eutectic solvent-supercritical technology with column chromatography. Background Art

[0002] Ginseng essential oil, also known as ginseng volatile oil, is a mixture of volatile substances extracted from the rhizome of ginseng and has a special fragrance of ginseng. Research shows that ginseng essential oil has relatively wide biological activities such as anti-inflammatory, antitussive, anti-fatigue, lipid-lowering, hangover prevention, central nervous system excitation, and tumor inhibition. The components contained in ginseng essential oil are complex and numerous, and the main chemical components include terpenoids, alcohols, ketones, aldehydes, phenols, heterocyclic compounds, and alkane compounds, etc. Among them, the research on polyacetylene alcohols represented by panaxynol is relatively in-depth.

[0003] Panaxynol, also known as falcarinol, has the chemical name of (9 Z )-1,9-diene-4,6-diyne-heptadec-3-ol, with the molecular formula C 17 H 24 O and a molecular weight of 244.38. The structural formula of panaxynol is as follows:

[0004] .

[0005] Panaxynol belongs to low-polar polyacetylene components and is mainly distributed in plants such as Araliaceae, Umbelliferae, and Compositae. Research shows that panaxynol has various pharmacological effects such as anti-cancer, anti-coagulation, inhibition of prostaglandin degradation, antibacterial, blood pressure lowering, sedation, analgesia, prevention of atherosclerosis, nerve cell protection, and anti-aging. Polyacetylene components represented by panaxynol and panaxydol are the most abundant in ginseng, accounting for more than 90% of the total polyacetylene content.

[0006] Due to the presence of double bonds and triple bonds in the structure of polyacetylene compounds, they are unstable in the presence of light or heat. Different extraction and separation methods directly affect the yield of polyacetylene components and thus affect the pharmacological activity of ginseng essential oil.

[0007] The traditional extraction methods of ginseng essential oil include steam distillation method, solvent extraction method, supercritical CO 2 extraction method, etc., which are specifically as follows:

[0008] 1. Steam distillation method

[0009] After soaking the raw material coarse powder in water in a distiller, it is directly heated for distillation, or the raw material is placed on a perforated partition mesh. When the steam generated by heating the water at the bottom passes through the raw material, the volatile oil is distilled out simultaneously with the water vapor by heating. The distillate is collected and the oil layer is separated after cooling. However, the raw material is easily carbonized by strong heat, which may change the components, cause the volatile oil to change its taste, reduce its value as a spice, and it is difficult to separate the water vapor contained in the volatile oil with low polarity.

[0010] 2. Solvent extraction method

[0011] It is extracted with organic solvents such as petroleum ether (30 - 60 °C), carbon disulfide, and carbon tetrachloride. The extraction method can adopt the reflux extraction method or the cold soaking method. After the organic solvent is evaporated under reduced pressure, an extract is obtained. Then the extract is dissolved in hot ethanol, and the essential oil is obtained after recovering the ethanol. The biggest drawback of this method is the risk of possible solvent residues, which requires a strict purification process; the organic solvents used may be toxic and flammable, and strict safety measures are required for operation; the use and discharge of solvents may have an impact on the environment.

[0012] 3. Supercritical CO 2 Extraction method

[0013] Media such as carbon dioxide, ethane, and nitrous oxide are used to reach the supercritical state through a certain temperature and pressure for extracting the aromatic volatile components in medicinal materials. However, the effect of extracting essential oil and panaxynol from ginseng by this method needs to be further improved. For example, the prior art (Zuo Xu. Research on the anti-inflammatory activity of volatile oil from ginseng [D]. Jilin University) discloses the extraction of ginseng volatile oil by supercritical CO 2 The yield is only 0.5%.

[0014] Currently, in the existing prior art, the yield of ginseng volatile oil is all below 3%, and at the same time, the content of panaxynol needs to be further improved.

[0015] Therefore, it is very necessary to develop a method for extracting ginseng essential oil by combining eutectic solvent - supercritical technology with column chromatography to solve the above technical problems. Summary of the invention

[0016] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a method for extracting ginseng essential oil by combining eutectic solvent - supercritical technology with column chromatography, which has a high yield, a high content of panaxynol, a short extraction period, and does not cause environmental pollution.

[0017] The present invention is realized through the following technical solutions:

[0018] A method for extracting ginseng essential oil by combining eutectic solvent - supercritical technology with column chromatography, comprising the following steps:

[0019] (1) Grind the ginseng roots and add them into a supercritical extraction kettle. 2 and an entrainer to perform supercritical extraction to obtain an extract; the entrainer comprises anhydrous ethanol and a low eutectic solvent;

[0020] (2) The extract is concentrated until there is no ethanol, diluted with water, separated by column chromatography, adsorbed on a macroporous resin for column chromatography, washed with water, eluted with 2%-5% ethanol, and eluted with 10%-20% ethanol in sequence, and the 10%-20% ethanol eluate is collected and dried.

[0021] As an embodiment of the present invention, the water content of the ginseng roots in step (1) is not higher than 9%, which can be achieved by the following operations: washing the fresh ginseng roots, and drying them by low-temperature drying at a temperature of 30°C-60°C to avoid loss of ginseng essential oil. The water content after drying is not higher than 9%.

[0022] As an embodiment of the present invention, in step (1), the ginseng roots are crushed and then passed through a 20-60 mesh sieve.

[0023] As an embodiment of the present invention, the supercritical extraction in step (1) is performed at a pressure of 15 MPa-45 MPa, a temperature of 25° C.-60° C., and a time of 1-2 h.

[0024] As an embodiment of the present invention, the volume ratio of the anhydrous ethanol to the low eutectic solvent is 9:1-15:1.

[0025] As an embodiment of the present invention, the low eutectic solvent is an anhydrous system.

[0026] As an embodiment of the present invention, the dosage ratio of ginseng root to entraining agent is 1 g / mL-6 g / mL.

[0027] As an embodiment of the present invention, the liquid CO 2 The flow rate is 100-140 L / h.

[0028] As a preferred embodiment of the present invention, step (1) is as follows:

[0029] The ginseng roots were crushed and added to the supercritical extraction kettle. CO was continuously introduced into the supercritical extraction kettle. 2 , slowly increase the pressure of the supercritical extraction kettle to 15MPa-45MPa, and the extraction temperature to 25℃-60℃; after the pressure and temperature of the extraction kettle reach the rated value, slowly open the vent valve, remove the air in the kettle, and then close the vent valve; input liquid CO into the extraction kettle 2 and an entrainer for supercritical extraction to obtain an extract.

[0030] As an embodiment of the present invention, the eutectic solvent includes at least one combination among the following combinations:

[0031] ①Combination A containing polyol and saccharide;

[0032] ②Combination B containing polyol and carboxylic acid;

[0033] ③Combination C containing at least two polyols;

[0034] ④Combination D containing quaternary ammonium salt and hydrogen bond donor;

[0035] ⑤Combination E containing carboxylic acid and saccharide.

[0036] As a preferred embodiment of the present invention, the hydrogen bond donor includes at least one of polyol, saccharide, urea, and carboxylic acid.

[0037] As a preferred embodiment of the present invention, the polyol includes at least one of glycerol, propylene glycol, and xylitol.

[0038] As a preferred embodiment of the present invention, the saccharide includes at least one of fructose, maltose, sucrose, lactose, galactose, and glucose.

[0039] As a preferred embodiment of the present invention, the carboxylic acid includes at least one of malic acid, citric acid, and lactic acid.

[0040] As a preferred embodiment of the present invention, the quaternary ammonium salt includes choline chloride.

[0041] As a preferred embodiment of the present invention, the molar ratio of polyol to saccharide in combination A is 1-4:1-4, and more preferably 1-3:1.

[0042] As a preferred embodiment of the present invention, the molar ratio of polyol to carboxylic acid in combination B is 1-4:1-4, and more preferably 1:1.

[0043] As a preferred embodiment of the present invention, the molar ratio of two polyols in combination C is 1-4:1-4, and more preferably 1:1.

[0044] As a preferred embodiment of the present invention, the molar ratio of quaternary ammonium salt to hydrogen bond donor in combination D is 1-4:1-4.

[0045] As a more preferred embodiment of the present invention, the molar ratio of quaternary ammonium salt to polyol in combination D is 1-2.5:1-4.

[0046] As a more preferred embodiment of the present invention, the molar ratio of quaternary ammonium salt to saccharide in combination D is 1-4:1.

[0047] As a more preferred embodiment of the present invention, the molar ratio of the quaternary ammonium salt to urea in combination D is 1:2.

[0048] As a more preferred embodiment of the present invention, the molar ratio of the quaternary ammonium salt to the carboxylic acid in combination D is 1-2:1-4.

[0049] As a preferred embodiment of the present invention, the molar ratio of the carboxylic acid to the saccharide in combination E is 1-4:1-4, and more preferably 1-2:1.

[0050] As a preferred embodiment of the present invention, step (2) is as follows:

[0051] The extract is concentrated by vacuum distillation until there is no ethanol, diluted 20 times with water, and then subjected to column chromatography separation. It is loaded onto macroporous resin for adsorption column chromatography, and successively washed with water, eluted with 2%-5% ethanol, and eluted with 10%-20% ethanol. The 10%-20% ethanol eluate is collected and dried to obtain the product.

[0052] As an embodiment of the present invention, the macroporous resin in step (2) includes at least one of D101 type, AB-8 type, and D4020 type macroporous resins.

[0053] As an embodiment of the present invention, the drying method in step (2) is vacuum evaporation to dryness.

[0054] The beneficial effects of the present invention are as follows:

[0055] The present invention first adopts the eutectic solvent-supercritical technology combined with column chromatography technology. The yield of ginseng essential oil obtained by extraction is greater than 1.0%, and the content of panaxynol is relatively high, with the content of panaxynol > 10%.

[0056] By drying and pre-treating ginseng rootlets, the present invention improves the yield of ginseng essential oil and the content of panaxynol in the ginseng essential oil within a specific water content range.

[0057] The present invention further optimizes the volume ratio of absolute ethanol to the eutectic solvent and the composition of the eutectic solvent, further improving the yield of ginseng essential oil and the content of panaxynol in the ginseng essential oil.

[0058] The method of the present invention has a high yield, few impurities, a short extraction time, does not cause environmental pollution, and can efficiently prepare ginseng essential oil rich in panaxynol. Description of the Drawings

[0059] Figure 1 It is the chromatogram of the ginseng essential oil sample obtained under the composition of the DES-1 eutectic solvent in Example 1, and the ordinate (%) represents the relative abundance.

[0060] Figure 2 It is the chromatogram of the panaxynol reference substance in Example 1, and the ordinate (%) represents the relative abundance.

[0061] Figure 3 It is the chromatogram of the ginseng essential oil sample obtained in Example 2, and the ordinate (%) represents the relative abundance.

[0062] Figure 4 It is the chromatogram of the panaxynol reference substance in Example 2, and the ordinate (%) represents the relative abundance.

[0063] Figure 5 It is the chromatogram of the ginseng essential oil sample obtained in Example 3, and the ordinate (%) represents the relative abundance.

[0064] Figure 6 It is the chromatogram of the panaxynol reference substance in Example 3, and the ordinate (%) represents the relative abundance. Detailed implementation manners

[0065] The present invention will be further described below in conjunction with specific embodiments, and the advantages and features of the present invention will become clearer as the description proceeds. However, these embodiments are exemplary only and do not constitute any limitation to the scope of the present invention. Those skilled in the art should understand that the details and forms of the technical solutions of the present invention can be modified or replaced without departing from the spirit and scope of the present invention, but such modifications and replacements all fall within the protection scope of the present invention.

[0066] Example 1

[0067] A method for extracting ginseng essential oil by combining eutectic solvent-supercritical technology with column chromatography, comprising the following steps:

[0068] (1) Wash the fresh ginseng root whiskers, and perform drying treatment by means of low-temperature drying. The drying temperature is 45 °C, and the water content after drying is 5%.

[0069] (2) After crushing the dried ginseng root whiskers and passing through a 40-mesh sieve, add them to the supercritical extraction kettle, and continuously input CO 2 into the supercritical extraction kettle, slowly increase the pressure of the supercritical extraction kettle to make the extraction pressure reach 30 MPa, the extraction temperature is 45 °C, and the extraction time is 1.5 h; after the pressure and temperature of the extraction kettle reach the rated values, slowly open the air release valve, exhaust the air in the kettle, and then close the air release valve; input liquid CO 2 and entrainer into the extraction kettle for supercritical extraction to obtain an extract;

[0070] The ratio of the dried ginseng root whiskers to the usage amount of the entrainer is 1 g / 0.3 mL. The entrainer is a combination of anhydrous ethanol and eutectic solvent. The volume ratio of anhydrous ethanol to eutectic solvent is 12:1. The composition of the eutectic solvent is shown in Table 1; the liquid CO2 The flow rate is 120 L / h.

[0071] Table 1 Composition of the deep eutectic solvent

[0072]

[0073] (3) The extract was concentrated by vacuum distillation until there was no ethanol. After diluting 20 times with water, column chromatography separation was used. It was adsorbed on D101 macroporous resin, washed with water, eluted with 2% ethanol, and then eluted with 10% ethanol. The 10% ethanol eluate was collected and evaporated to dryness under reduced pressure to obtain ginseng essential oil.

[0074] Example 2

[0075] A method for extracting ginseng essential oil by combining deep eutectic solvent-supercritical technology with column chromatography, comprising the following steps:

[0076] (1) Wash the fresh ginseng rootlets, and dry them by low-temperature drying. The drying temperature is 30 °C, and the water content after drying is 7%.

[0077] (2) After crushing the dried ginseng rootlets and passing through a 60-mesh sieve, add them to the supercritical extraction kettle. Continuously input CO 2 , slowly increase the pressure of the supercritical extraction kettle to make the extraction pressure reach 45 MPa, the extraction temperature is 25 °C, and the extraction time is 2 h. After the pressure and temperature of the extraction kettle reach the rated values, slowly open the bleed valve, exhaust the air in the kettle, and then close the bleed valve. Input liquid CO 2 and entrainer for supercritical extraction to obtain an extract;

[0078] The dosage ratio of the dried ginseng rootlets to the entrainer is 1:1 (g / mL). The entrainer is a combination of anhydrous ethanol and deep eutectic solvent (DES-5). The volume ratio of anhydrous ethanol to deep eutectic solvent (DES-5) is 9:1. The flow rate of the liquid CO 2 is 100 L / h;

[0079] (3) The extract was concentrated by vacuum distillation until there was no ethanol. After diluting 20 times with water, column chromatography separation was used. It was adsorbed on AB-8 macroporous resin for column chromatography, washed with water, eluted with 5% ethanol, and then eluted with 20% ethanol. The 20% ethanol eluate was collected and evaporated to dryness under reduced pressure to obtain ginseng essential oil.

[0080] Example 3

[0081] A method for extracting ginseng essential oil by combining deep eutectic solvent-supercritical technology with column chromatography, comprising the following steps:

[0082] (1) Wash the fresh ginseng rootlets, and perform drying treatment by means of low-temperature drying. The drying temperature is 60°C, and the water content after drying is 4%.

[0083] (2) Crush the dried ginseng rootlets and pass through a 20-mesh sieve, then add them to a supercritical extraction kettle. Continuously input CO 2 into the supercritical extraction kettle, slowly increase the pressure of the supercritical extraction kettle to make the extraction pressure reach 15 MPa, the extraction temperature is 60°C, and the extraction time is 1 h. After the pressure and temperature of the extraction kettle reach the rated values, slowly open the bleed valve, exhaust the air in the kettle, and then close the bleed valve. Input liquid CO 2 and an entrainer into the extraction kettle for supercritical extraction to obtain an extract.

[0084] The ratio of the dried ginseng rootlets to the entrainer is 6:1 (g / mL). The entrainer is a combination of absolute ethanol and a deep eutectic solvent (DES-10). The volume ratio of absolute ethanol to the deep eutectic solvent (DES-10) is 15:1. The flow rate of the liquid CO 2 is 140 L / h.

[0085] (3) Concentrate the extract by means of vacuum distillation until there is no ethanol. Dilute it 20 times with water, and then use column chromatography separation. Load it onto D4020 macroporous resin for adsorption column chromatography, and successively wash it with water, elute it with 3% ethanol, and elute it with 15% ethanol. Collect the 15% ethanol eluate and evaporate it to dryness under reduced pressure to obtain ginseng essential oil.

[0086] Comparative Example 1

[0087] The difference from Example 2 is only that the water content of the ginseng rootlets after drying is different, which is 20%, and the other conditions are the same. The specific steps of (1) are as follows:

[0088] (1) Wash the fresh ginseng rootlets, and perform drying treatment by means of low-temperature drying. The drying temperature is 30°C, and the water content after drying is 20%.

[0089] Comparative Example 2

[0090] The difference from Example 2 is only that the volume ratio of absolute ethanol to the deep eutectic solvent is different, which is 4:1, and the other conditions are the same.

[0091] Comparative Example 3

[0092] The difference from Example 2 is only that the volume ratio of absolute ethanol to the deep eutectic solvent is different, which is 30:1, and the other conditions are the same.

[0093] Comparative Example 4

[0094] It is only different from Example 1 in that the molar ratio of polyol and saccharide in the eutectic solvent is different. Specifically, it is a combination of glycerol and fructose, and the molar ratio of the two is 6:1, and the remaining conditions are the same.

[0095] Comparative Example 5

[0096] It is only different from Example 1 in that the molar ratio of polyol and saccharide in the eutectic solvent is different. Specifically, it is a combination of glycerol and fructose, and the molar ratio of the two is 1:5, and the remaining conditions are the same.

[0097] Test Example 1

[0098] Determination of the yield of ginseng essential oil and the content of panaxynol

[0099] (1) Materials and instruments

[0100] Both methanol and acetonitrile are of UPLC-MS grade; formic acid is of MS grade; leucine enkephalin and sodium formate are purchased from Waters Technologies Corporation, USA; other reagents are of analytical grade. Panaxynol is purchased from Bio CuiRan Biotechnology Co., Ltd.

[0101] Waters Xevo G2-S Q-Tof quadrupole time-of-flight mass spectrometer, ACQUITY UPLC ultra-high performance liquid chromatograph, MasslynxTM V4.1 workstation; N-A35 type nitrogen generator (Shanghai Jinlang Technology Co., Ltd.); PTX-FA2105 type electronic balance (Fujian Huazhi Electronic Technology Co., Ltd.).

[0102] (2) Experimental method

[0103] Preparation of test sample and reference substance solutions: Weigh 10 mg of the ginseng essential oil prepared in each example or comparative example, dissolve and make up the volume to 10 ml with methanol to obtain the test sample solution, and filter through a 0.22 μm microporous filter membrane for standby. Weigh 1 mg of the panaxynol reference substance respectively, dissolve and make up the volume to 10 ml with methanol to obtain the reference substance solution, and filter through a 0.22 μm microporous filter membrane for standby.

[0104] (3) Instrument conditions

[0105] Chromatographic conditions: Waters ACQUITY UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm), column temperature: 30 °C; sample manager temperature: 15 °C; flow rate: 1.5 ml / min; mobile phase A: 0.1% formic acid aqueous solution; mobile phase B: acetonitrile (0.1% formic acid); gradient elution program: 0 - 2 minutes, 30% A, 10% B; 2 - 26 minutes, 30% → 0% A, 70% → 100% B; 26 - 28 minutes, 0% A, 100% B; 28 - 29 minutes, 0% → 30% A, 100% → 70% B; 29 - 30 minutes, 30% A, 70% B.

[0106] Mass spectrometry conditions: Electrospray ionization (ESI) positive ion (ESI + ) mode, source temperature is 120 °C; desolvation temperature is 400 °C; cone voltage: 40 V; cone gas flow rate: 50 L / h; desolvent gas flow rate: 788.0 L / h; capillary voltage: 2.15 kV; use MSE mode to comprehensively collect mass spectrometry data, set the low-energy channel energy to 6 V, and the high-energy channel energy range to 20 V - 40 V; set the mass scanning range in the interval of m / z 150 - 290 Da; the real-time calibration solution is 100 ng / ml leucine enkephalin (m / z 556.2771 in ESI + ) mode, and the real-time calibration flow rate is 15 μl / min; the sample injection volume is 5 μl; use the Masslynx V4.1 workstation to be responsible for data processing and management.

[0107] (4) Content determination method

[0108] Record the peak area, and use the external standard method to determine the content of panaxynol in the panax essential oil sample. Weigh the mass of the panax essential oil and calculate the yield of the panax essential oil.

[0109] (5) Experimental results

[0110] The experimental results of Example 1 are shown in Table 2, and the experimental results of Example 2, Example 3 and Comparative Examples 1 - 5 are shown in Table 3.

[0111] Table 2 Yield of panax essential oil and content of panaxynol in Example 1

[0112]

[0113] Table 3 Yield of panax essential oil and content of panaxynol in Example 2, Example 3 and Comparative Examples 1 - 5

[0114]

[0115] The chromatograms of the ginseng essential oil sample and the panaxynol reference substance under the DES-1 eutectic solvent composition in Example 1 are shown in Figure 1 and Figure 2 .

[0116] The chromatograms of the ginseng essential oil sample and the panaxynol reference substance obtained in Example 2 are shown in Figure 3 and Figure 4 , and the chromatograms of the ginseng essential oil sample and the panaxynol reference substance obtained in Example 3 are shown in Figure 5 and Figure 6 .

[0117] The above detailed description is a specific description of one of the feasible embodiments of the present invention, and this embodiment is not intended to limit the patent scope of the present invention. Any equivalent implementation or change made without departing from the present invention shall be included within the scope of the technical solution of the present invention.

Claims

1. A method for extracting ginseng essential oil by deep eutectic solvent-supercritical technology combined with column chromatography, characterized in that: The steps include: (1) Ginseng roots are crushed and added into a supercritical extraction kettle, and supercritical extraction is performed under the combined action of liquid CO2 and an entrainer to obtain an extract; the entrainer includes anhydrous ethanol and a low eutectic solvent; the water content of the ginseng roots is not higher than 9%; (2) Concentrate the extract until there is no ethanol, dilute with water, apply macroporous resin for adsorption and column chromatography, wash with water, elute with 2%-5% ethanol, and elute with 10%-20% ethanol in sequence, collect the 10%-20% ethanol eluate, and dry it to obtain the product; The volume ratio of anhydrous ethanol to the deep eutectic solvent is 9:1-15:1; The deep eutectic solvent is an anhydrous system; The deep eutectic solvent comprises at least one of the following combinations: ① Combination A comprising polyols and sugars; the molar ratio of polyols to sugars in combination A is 1-4:1-4; ② Combination B comprising polyol and carboxylic acid; the molar ratio of polyol to carboxylic acid in combination B is 1-4:1-4; ③ A combination C comprising at least two polyols; the molar ratio of the two polyols in the combination C is 1-4:1-4; ④ A combination D comprising a quaternary ammonium salt and a hydrogen bond donor; the molar ratio of the quaternary ammonium salt to the hydrogen bond donor in the combination D is 1-4:1-4; ⑤ A combination E comprising carboxylic acid and sugar; the molar ratio of carboxylic acid to sugar in combination E is 1-4:1-4.

2. The method according to claim 1, characterized in that The hydrogen bond donor comprises at least one of polyols, sugars, urea and carboxylic acids.

3. The method according to claim 1 or 2, characterized in that: The polyol includes at least one of glycerol, propylene glycol and xylitol; the sugar includes at least one of fructose, maltose, sucrose, lactose, galactose and glucose; the carboxylic acid includes at least one of malic acid, citric acid and lactic acid; and the quaternary ammonium salt includes choline chloride.

4. The method according to claim 1, characterized in that The molar ratio of polyols to sugars in combination A is 1-3:1; the molar ratio of polyols to carboxylic acids in combination B is 1:1; the molar ratio of the two polyols in combination C is 1:1; and the molar ratio of carboxylic acids to sugars in combination E is 1-2:

1.

5. The method according to claim 1, characterized in that In step (1), the ginseng roots are crushed and passed through a 20-60 mesh sieve.

6. The method according to claim 1, characterized in that The supercritical extraction pressure in step (1) is 15MPa-45MPa, and the temperature is 25°C-60°C; the macroporous resin in step (2) includes at least one of D101, AB-8 and D4020 macroporous resins.

7. The method according to claim 1, characterized in that The dosage ratio of the ginseng root to the entraining agent is 1g / mL-6g / mL, and the flow rate of the liquid CO2 is 100-140 L / h.

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