A strain of the genus Bosella, a bacterial agent, uses and methods

Abstract

A Bosea sp. strain, bacterial agent, use and method. The Bosea sp. strain provided in the present application is the Bosea sp. QY2 strain, and its deposit number is CCTCC NO: M 20242096. The Bosea sp. QY2 strain of the present application can grow with polyethylene as the sole carbon source, and can tolerate polyethylene plastics at a concentration of 1-1000 mg / L. The strain can significantly reduce the infrared spectral characteristic functional groups and characteristic products identified by pyrolysis chromatography-mass spectrometry of polyethylene plastics, and the strain can change the thermodynamic properties of polyethylene, indicating that the Bosea sp. QY2 strain can utilize polyethylene plastics. The discovery of this strain provides new ideas and methods for degrading polyethylene plastics in various environments, which is beneficial to the control of environmental pollution and human health.

Description

Technical Field

[0001] The present invention generally relates to microbial technology, and in particular to a Bosella QY2 strain, a bacterial agent containing the same, a use thereof, and a method for degrading polyethylene. Background Art

[0002] With the development of the chemical industry, plastic products, due to their lightness, durability, affordability, and convenience, have become widely used in various packaging materials (polyethylene, PE), beverage bottles (polyester, PET), foam padding, and fast food lunch boxes (polystyrene, PS). Plastic pollution is one of the most pressing ecological and health issues globally. Because plastics are mostly composed of stable, long-chain polymers, they are not easily degraded in the environment, and the degradation process is extremely slow.

[0003] The chemical structure of polyethylene consists of repeating -[CH2-CH2] n -unit composition, this material is stable due to its large molecular weight and strong hydrophobicity. In particular, its strong CC chain skeleton and the lack of enzymes in nature to break down these chains make it difficult to biodegrade under natural conditions, making it easy to accumulate in the environment. At present, the methods for treating waste plastics mainly include landfill, burning and reprocessing. These methods not only cause serious pollution to the environment, but also have high treatment costs and may cause secondary pollution problems. In view of this, it is particularly important to explore safe and green treatment technologies for polyethylene plastic waste. At present, there are relatively few studies on the biological treatment of polyethylene plastic waste, the resources of efficient bacterial strains are limited, and the treatment efficiency is low. The development of strains that can degrade polyethylene plastics is an urgent problem that needs to be solved. Summary of the Invention

[0004] Based on this, the present application provides a Bosea sp. strain capable of degrading polyethylene, wherein the Bosea sp. strain is Bosea sp. QY2 strain, and its deposit number is CCTCC NO: M20242096.

[0005] In another aspect, the present application also provides a bacterial agent comprising the Bosteria QY2 strain described herein.

[0006] In another aspect, the present application also provides use of the Bosella QY2 strain described herein or the bacterial agent described herein in degrading polyethylene.

[0007] In another aspect, the present application also provides a method for degrading polyethylene, wherein the Bosella QY2 strain described herein or the bacterial agent described herein is co-cultured with polyethylene.

[0008] The present application provides a strain of the genus Bosea sp., designated Bosea sp. QY2. The strain was deposited with the China Center for Type Culture Collection, No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province, on September 26, 2024. The deposit number is CCTCC NO: M 20242096.

[0009] The Bosnerella QY2 strain described in this application can grow on polyethylene as its sole carbon source and tolerate polyethylene plastic concentrations of 1-1000 mg / L. This strain significantly reduces the number of characteristic infrared spectral functional groups and characteristic products identified by pyrolysis chromatography-mass spectrometry in polyethylene plastic, and can also alter the thermodynamic properties of polyethylene, demonstrating that Bosnerella QY2 can utilize polyethylene plastic. The discovery of this strain provides new insights and methods for degrading polyethylene plastic in various environments, potentially benefiting environmental pollution control and human health.

[0010] Other features and advantages of the present application will be described in the following description, and in part will become apparent from the description, or will be understood by practicing the present application. Other advantages of the present application can be realized and obtained by the solutions described in the description and the drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] The accompanying drawings are used to provide an understanding of the technical solution of the present application and constitute a part of the specification. Together with the embodiments of the present application, they are used to explain the technical solution of the present application and do not constitute a limitation on the technical solution of the present application.

[0012] Figure 1 The growth of the Bosella QY2 strain isolated and purified in this application in solid culture medium with or without the addition of high-density polyethylene (HDPE) aging solution is shown. The two pictures in the first row are the front of the culture dish, and the two pictures in the second row are the back of the culture dish.

[0013] Figure 2 The colony status of the Bosteria QY2 strain of the present application at different growth times in a solid culture medium with or without the addition of HDPE powder is shown.

[0014] Figure 3 This is an infrared spectrum of the treatment with the Bosella QY2 strain and the HDPE control in Example 2 of the present application.

[0015] Figure 4 This is the pyrolysis chromatogram mass spectrum of the treatment with Bosella QY2 strain and HDPE control in Example 2 of the present application.

[0016] Figure 5This is a differential scanning calorimetry (DSC) spectrum of the treatment with Bosella QY2 strain and HDPE control in Example 2 of the present application. DETAILED DESCRIPTION

[0017] Unless otherwise indicated, the technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art to which this application belongs. When a certain amount, concentration or other value or parameter is expressed in the form of a range, a preferred range, or a preferred upper numerical limit and a preferred lower numerical limit, it should be understood that it is equivalent to specifically revealing any range by combining any pair of upper range limits or preferred numerical values ​​with any lower range limit or preferred numerical value, without considering whether the range is specifically revealed. Unless otherwise indicated, the numerical ranges listed herein are intended to include the endpoints of the range and all integers and fractions (decimals) within the range.

[0018] The terms "about" and "approximately" when used in conjunction with a numerical variable generally refer to the value of that variable and all values ​​of that variable are within experimental error (e.g., within a 95% confidence interval about the mean) or within ±10% of the stated value, or wider.

[0019] The expression "comprising" or its synonymous similar expressions "including," "containing," and "having" are open-ended and do not exclude additional unrecited elements, steps, or ingredients. The expression "consisting of excludes any elements, steps, or ingredients not specified. The expression "consisting essentially of" means that the scope is limited to the specified elements, steps, or ingredients, plus any optional elements, steps, or ingredients that do not materially affect the basic and novel characteristics of the claimed subject matter. It should be understood that the expression "comprising" encompasses the expressions "consisting essentially of" and "consisting of."

[0020] The expression "at least one" or "one or more" means 1, 2, 3, 4, 5, 6, 7, 8, 9 or more.

[0021] The present application provides a Bosea sp. strain, wherein the Bosea sp. strain is Bosea sp. QY2 strain, and the deposit number is CCTCC NO: M 20242096.

[0022] In another aspect, the present application also provides a bacterial agent comprising the Bosteria QY2 strain described herein.

[0023] In some embodiments, the bacterial agents described herein comprise live bacteria of the Bordetella QY2 strain.

[0024] In some embodiments, the bacterial agents described herein contain the Bosteria sp. QY2 strain as the sole active ingredient.

[0025] In some embodiments, the bacterial agents described herein further comprise other active ingredients. In some embodiments, the bacterial agents described herein further comprise one or more other active ingredients that can act synergistically with the Bosnerella QY2 strain. In some embodiments, the other active ingredients are one or more other compounds or strains that can act synergistically with the Bosnerella QY2 strain. In some embodiments, the other active ingredients are one or more compounds that can react with plastics (e.g., polyethylene plastics, polystyrene plastics, or polyester plastics). In some embodiments, the other active ingredients are one or more other strains that can degrade plastics (e.g., polyethylene plastics, polystyrene plastics, or polyester plastics). Synergistic effects include promoting the growth and reproduction of the Bosnerella QY2 strain, enhancing the plastic degradation ability of the Bosnerella QY2 strain, and having a wider range of uses or functions after compounding with the Bosnerella QY2 strain.

[0026] In some embodiments, the bacterial compositions described herein further comprise other substances that provide conditions for the degradation of plastic by the Bordeauxella QY2 strain. In some embodiments, the bacterial compositions described herein further comprise a culture medium that provides nutrients other than a carbon source for the growth of the Bordeauxella QY2 strain. In some embodiments, the bacterial compositions described herein further comprise a culture medium that promotes the growth of the Bordeauxella QY2 strain. In some embodiments, the bacterial compositions described herein further comprise PPM culture medium.

[0027] In some embodiments, the formula of the solid plastic degrading bacteria purification medium (PPM medium) with plastic aging liquid added as the sole carbon source is 1g KH2PO4, 3g K2HPO4·3H2O, 0.2g MgSO4, 1g NaCl, 0.01g CaCl2, 0.006g Na2SeO35H2O, 0.008g Na2WO42H2O, 0.5g EDTA, 0.2g 7H2O·Fe2SO4, 0.01g 7H2O·ZnSO4, 0.003g 4H2O·MnCl2, 0.03g H3BO3, 0.02g 6H2O·CoCl2, 0.001g 2H2O·CuCl2, 0.002g 6H2O·NiCl2, 0.003g 2H2O·NaMoO4, 15g agar and 1L ultrapure water, as well as the plastic aging solution added after high temperature and high pressure sterilization and cooling.

[0028] In another aspect, the present application also provides use of the Bosella QY2 strain described herein or the bacterial agent described herein in degrading polyethylene.

[0029] In some embodiments, the Bosella QY2 strain described herein can degrade polyethylene alone in the environment. In some embodiments, the Bosella QY2 strain described herein can be used together with other microorganisms to degrade polyethylene in the environment. In some embodiments, the Bosella QY2 strain described herein can be used together with other active ingredients in the bacterial agent described herein to degrade polyethylene. In some embodiments, the Bosella QY2 strain described herein can be used together with one or more other strains capable of degrading plastics (e.g., polyethylene plastics, polystyrene plastics, or polyester plastics) to treat plastic pollutants in the environment. In some embodiments, the Bosella QY2 strain described herein can be used together with one or more other strains capable of degrading other pollutants to treat various pollutants in the environment.

[0030] In some embodiments, bacteria that can cooperate with the Bosella QY2 strain of the present application include Pseudomonas sp., Chryseobacterium sp., Alternaria sp., and Bacillus sp., etc.

[0031] In some embodiments, the degradation is that the strain QY2 of the genus Bosella grows on polyethylene as the sole carbon source and gradually degrades the polyethylene.

[0032] In some embodiments, the polyethylene is selected from one or more of low density polyethylene, medium density polyethylene, and high density polyethylene.

[0033] In some embodiments, the polyethylene is high density polyethylene.

[0034] In some embodiments, the polyethylene is polyethylene granules, films, or powders. In some embodiments, the polyethylene comprises at least one of various particle sizes and specifications. In some embodiments, the plastic comprises plastics of various types and sources. In some embodiments, the polyethylene plastic is aged plastic.

[0035] In some embodiments, the Bosnerella QY2 strain can degrade polyethylene in various water bodies (e.g., river water, lake water, ocean water, or sewage). In some embodiments, the Bosnerella QY2 strain can degrade plastic in the form of micro-nanoparticles in various water bodies (e.g., river water, lake water, ocean water, or sewage). In some embodiments, the Bosnerella QY2 strain can degrade polyethylene in the soil of contaminated sites. In some embodiments, the Bosnerella QY2 strain can degrade polyethylene in landfills.

[0036] In some embodiments, the Bosella QY2 strain can be used together with other strains to degrade sediments in different water bodies (e.g., river water, lake water, oceans, or sewage). In some embodiments, the Bosella QY2 strain can be used together with other strains to degrade plastics in the form of micro-nanoparticles in different water bodies (e.g., river water, lake water, oceans, or sewage). In some embodiments, the Bosella QY2 strain can be used together with other strains to degrade various garbage pollutants in landfills. In some embodiments, the Bosella QY2 strain can produce a synergistic effect with other strains and be used together to treat sewage and / or contaminated soil. In some embodiments, the Bosella QY2 strain can produce a synergistic effect with other strains and be used together for the degradation of garbage in landfills and the recycling of matter and energy.

[0037] In another aspect, the present application also provides a method for degrading polyethylene, wherein the Bosella QY2 strain described herein or the bacterial agent described herein is co-cultured with polyethylene.

[0038] This application describes multiple embodiments, but this description is exemplary rather than restrictive, and it will be apparent to those skilled in the art that there may be more embodiments and implementations within the scope of the embodiments described herein. Although many possible feature combinations are shown in the drawings and discussed in the detailed description, many other combinations of the disclosed features are also possible. Unless specifically limited, any feature or element of any embodiment may be used in combination with any other feature or element in any other embodiment, or may replace any other feature or element in any other embodiment.

[0039] The present application includes and contemplates combinations of features and elements known to those of ordinary skill in the art. The embodiments, features, and elements disclosed in this application may also be combined with any conventional features or elements to form a unique inventive solution. Any features or elements of any embodiment may also be combined with features or elements from other inventive solutions to form another unique inventive solution. Therefore, it should be understood that any feature shown and / or discussed in this application may be implemented individually or in any appropriate combination. Therefore, except for the limitations made according to the appended claims and their equivalents, the embodiments are not subject to other limitations. In addition, various modifications and changes may be made within the scope of protection of the appended claims.

[0040] In addition, when describing representative embodiments, the specification may have presented the method and / or process as a specific sequence of steps. However, to the extent that the method or process does not rely on the specific order of the steps described herein, the method or process should not be limited to the steps in the specific order described. As will be understood by those skilled in the art, other orders of steps are also possible. Therefore, the specific order of the steps set forth in the specification should not be interpreted as a limitation to the claims. In addition, the claims for the method and / or process should not be limited to performing their steps in the order written, and those skilled in the art can readily understand that these orders can be changed and still remain within the spirit and scope of the embodiments of the present application.

[0041] The experimental methods in the following examples, for which specific conditions are not specified, are generally determined in accordance with national standards. The experimental materials in the following examples, for which the sources are not specified, are all commercially available raw materials. The equipment used in each step in the following examples is all conventional equipment. If there are no corresponding national standards, the methods are carried out in accordance with general international standards, conventional conditions, or conditions recommended by the manufacturer. Unless otherwise defined or indicated, all professional and scientific terms used in this application have the same meaning as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein can be applied to the methods of this application.

[0042] Example

[0043] The relevant materials used in the examples are as follows:

[0044] 1. Reagents and Materials

[0045] (NH4)2SO4, KNO3, NaCl, C5H 14 ClNO, C 18 H 32 CaN2O 10 、C 19 H 19 N7O6、C6H6N2O、C8H9NO3、C 17 H 20 N4O6、C 12 H 17 ClN4OS·HCl and C6H 12All reagents in the culture medium formulation, including O₂, KH₂PO₄, K₂HPO₄·3H₂O, MgSO₄, NaCl, CaCl₂, Na₂SeO₃₅5H₂O, Na₂WO₄₂2H₂O, EDTA, 7H₂O·Fe₂SO₄, 7H₂O·ZnSO₄, 4H₂O·MnCl₂, H₃BO₃, 6H₂O·CoCl₂, 2H₂O·CuCl₂, 6H₂O·NiCl₂, and 2H₂O·NaMoO₄, were of analytical grade. BG11 culture medium was purchased from Sinopharm Group, Qingdao Haibo Biotechnology, Macklin, Aladdin, and Sigma. Purified agar was used. Microplastics were 80-mesh high-density polyethylene (Sinopec).

[0046] 2. Instruments

[0047] Conventional culture equipment includes a high-temperature autoclave, clean bench, shaker, and constant-temperature incubator. A 395nm LED ultraviolet lamp was used to irradiate the aged microplastics. Testing instruments include an infrared spectrometer-microscope (Thermoelectric (Shanghai) Instrument Co., Ltd., Nicolet 6700FTIR), a pyrolysis chromatography-mass spectrometer (Shimadzu, GCMS-QP2010SE), and a differential scanning calorimeter (TA Instruments, Q5000IR).

[0048] Example 1. High-throughput plastic-degrading bacteria screening method to isolate polyethylene-degrading bacteria

[0049] This application utilizes a high-throughput enrichment method for plastic-degrading bacteria. This method first involves adding mineral water to contaminated site soil and oscillating the suspension for 24 hours to activate the bacteria. This suspension is then added to a liquid plastic-degrading bacteria enrichment medium (BG11 medium) for activation, followed by the addition of a pretreated polyethylene solution and oscillation for 48 hours. Finally, the polyethylene-degrading bacteria are further isolated and purified in a solid plastic-degrading bacteria purification medium (PPM medium) containing aged polyethylene solution as the sole carbon source. The specific steps are as follows.

[0050] During polyethylene pretreatment, polyethylene micro-nano plastics were added to 100 mL of water to prepare a 10 g / L plastic mother liquor; under an LED lamp (395 nm wavelength) simulating natural ultraviolet radiation, the beaker was placed in a magnetic stirrer and stirred continuously to simulate environmental aging, and incubated at room temperature of 25°C for 7 days.

[0051] Commercially available BG11 culture medium powder was used to prepare liquid plastic-degrading bacteria enrichment culture medium. The formula of the solid plastic-degrading bacteria purification culture medium (PPM culture medium) with plastic aging liquid as the sole carbon source is: 1g KH2PO4, 3g K2HPO4·3H2O, 0.2g MgSO4, 1g NaCl, 0.01g CaCl2, 0.006g Na2SeO35H2O, 0.008g Na2WO42H2O, 0.5g EDTA, 0.2g 7H2O·Fe2SO4, 0.01g 7H2O·ZnSO4, 0.003g 4H2O·MnCl2, 0.03g H3BO3, 0.02g 6H2O·CoCl2, 0.001g 2H2O·CuCl2, 0.002g 6H2O·NiCl2, 0.003g 2H2O·NaMoO4, 15g agar, and 1L ultrapure water. When preparing BG11 and PPM culture media, mix the culture medium powder thoroughly and dissolve it in ultrapure water. After high temperature and high pressure sterilization, BG11 culture medium can be used directly after cooling. PPM culture medium needs to be cooled to about 60°C and mixed with plastic mother liquor. Pour it into the wells of the microplate and cool and solidify for use.

[0052] Taking high-density polyethylene (HDPE) as an example, the process of separating plastic-degrading bacteria by the orifice plate plastic enrichment method of the present application is explained. First, 10 g of soil from a plastic-contaminated site (isolated from a garbage dump in Beijing) and 100 mL of Nongfu Spring Changbai Mountain mineral water were added to a conical flask, and cultured on a shaker at 30°C and 180 rpm / min for 24 hours to prepare a soil bacterial suspension; then 100 μL of soil bacterial suspension and 800 μL of low-nutrient liquid plastic-degrading bacteria enrichment medium (BG11 medium) were added to each well of a 48-well plate and activated by shaking for 24 hours; 100 μL of aged 80-mesh polyethylene mother liquor (the final concentration of polyethylene was 1000 mg / L) was added, and the culture was shaken at 30°C and 80 rpm / min. After 48 hours of culture, the degradation bacteria were further domesticated; finally, polyethylene mother liquor was added to the PPM medium to make the final concentration of polyethylene 1000 mg / L, and no polyethylene was added as a carbon-free and nitrogen-free control. The degradation bacteria domesticated in the previous step were inoculated and streaked onto the solid PPM medium, and cultured at 30°C for 7-20 days. The growth of bacterial colonies was observed and photographed. Bacteria that can form colonies on PPM plates with polyethylene as the only carbon source but cannot grow in solid culture media without plastic are judged to have polyethylene degradation potential; single colonies with plastic degradation potential are selected and separated and purified multiple times to finally obtain pure polyethylene-degrading bacteria. The growth of the strain in the culture medium with or without the addition of HDPE aging liquid as a carbon source is shown in the figure. Figure 1The strain was identified as Bosea sp. by 16S rDNA sequencing (its 16S rDNA sequence is shown in SEQ ID NO: 1) and named as Bosea sp. QY2.

[0053] It was deposited in China Center for Type Culture Collection, address: No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province, on September 26, 2024. The deposit number is CCTCC NO: M 20242096.

[0054] Example 2. Growth of Bosella QY2 strain in plastic degradation medium

[0055] The growth of Bosnia QY2 strain in 80 mesh high-density polyethylene was verified in a solid plastic degradation medium (Plastic Degrading Medium, PDM medium) with plastic powder added as the only carbon source. Single colonies of Bosnia QY2 strain were picked and streaked on plates with or without 1000 mg / L HDPE plastic added. They were cultured in a 30°C constant temperature shaker for 2-10 days. The growth of the colonies was observed and photographed to determine the concentration range of polyethylene that can grow colonies. The growth results of Bosnia QY2 strain are shown in Figure 2. Figure 2 The results show that Bostella QY2 cannot grow in a carbon-free, nitrogen-free medium without polyethylene plastic, but grows well on 1000 mg / L polyethylene plastic, forming distinct colonies. This indicates that Bostella QY2 can grow using polyethylene as its sole carbon source. Furthermore, Bostella QY2 cultured in PDM medium served as a treatment, while PDM medium alone served as a HDPE control. Both cultures were used in Examples 3-5 to further validate the strain's plastic degradation potential.

[0056] PDM medium was prepared as follows: 1 g KH2PO4, 3 g K2HPO4·3H2O, 0.2 g MgSO4, 1 g NaCl, 0.01 g CaCl2, 0.006 g Na2SeO35H2O, 0.008 g Na2WO42H2O, 0.5 g EDTA, 0.2 g 7H2O·Fe2SO4, 0.01 g 7H2O·ZnSO4, 0.003 g 4H2O·MnCl2, 0.03 g H3BO3, 0.02 g 6H2O·CoCl2, 0.001 g 2H2O·CuCl2, 0.002 g 6H2O·NiCl2, 0.003 g 2H2O·NaMoO4, and 15 g agar were weighed, mixed thoroughly, and brought to a volume of 1 L with ultrapure water. After high temperature and high pressure sterilization, add HDPE microplastic particles and mix evenly, then cool and solidify for later use.

[0057] Example 3. Infrared spectroscopy identification of polyethylene degradation by Bosella QY2 strain

[0058] The infrared spectrum results are as follows Figure 3 As shown, the HDPE control is at about 2918 cm -1 and 2853cm -1 There are strong double peaks at 1468 cm, which belong to the asymmetric and symmetric stretching vibrations of –CH2. The bending or rocking deformation band occurs at about 1468 cm -1 and 718cm -1 These characteristic functional groups of HDPE disappeared after being treated with the plastic-degrading bacteria Bordetella QY2, indicating that there was no HDPE plastic in the degradation system and that the plastic-degrading bacteria Bordetella QY2 could effectively reduce the content of HDPE.

[0059] Example 4. Pyrolysis Chromatography-Mass Spectrometry Identification of Polyethylene Degradation by Bosella QY2

[0060] The results of pyrolysis chromatography mass spectrometry were as follows Figure 4 As shown, the HDPE control produced a series of triplet peaks consisting of n-alkadienes (dienes), n-alkenes (alkenes), and n-alkanes (alkanes). Among them, n-alkenes was the largest of the three peaks. However, these peaks were almost undetectable after treatment with Bosnerella QY2, indicating that HDPE was almost absent in the treatment system with the added degrading bacteria, and that the degrading bacteria strain Bosnerella QY2 was capable of degrading polyethylene plastics.

[0061] Example 5. Differential Scanning Calorimetry Identification of Bosella QY2 Strain

[0062] Figure 5 The peak changes during heating and cooling scans for an HDPE control and treatments containing both plastic and degrading bacteria are shown, demonstrating the impact of plastic-degrading bacteria on the thermal properties of the HDPE matrix, such as crystallization and melting. The DSC curve for the HDPE control crystallizes at ~100°C and melts at ~125°C. However, the crystallization and melting temperatures of the treatment containing the Bordetella QY2 strain significantly shift, indicating that Bordetella QY2 alters the thermodynamic properties of HDPE plastic and has a certain ability to utilize HDPE plastic.

[0063] In summary, this application has identified a strain capable of degrading polyethylene through screening of soil from contaminated sites. 16S rDNA sequencing revealed that the strain belongs to the genus Bosea sp. and was named Bosea sp. QY2. Functional assays revealed that the Bosea sp. QY2 strain can tolerate 1000 mg / L of polyethylene plastic, reducing or eliminating the characteristic functional groups and pyrolysis-prone substances in polyethylene plastic and altering its thermodynamic properties, indicating that the Bosea sp. QY2 strain has a certain ability to utilize plastic. The discovery of this strain provides new ideas and methods for degrading polyethylene plastic in various environments, which is beneficial for the management of polyethylene plastic pollution and human health.

[0064] Although the embodiments of the present application have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations on the present application. Ordinary technicians in this field can change, modify, replace and modify the above embodiments within the scope of the present application.

Claims

1. A strain of the genus Bosella, characterized in that The Bostonia strain is the Bostonia QY2 (Boseasp.QY2) strain, and its preservation number is CCTCC NO: M 20242096.

2. A bacterial agent comprising the Bosteria QY2 strain according to claim 1.

3. The microbial agent according to claim 2, characterized in that The bacterial agent contains live bacteria of the Bosella QY2 strain.

4. The microbial agent according to claim 2, characterized in that The bacterial agent further comprises one or more other active ingredients capable of synergistically acting with the Bosella QY2 strain.

5. Use of the Bosella QY2 strain according to claim 1 or the bacterial agent according to any one of claims 2 to 4 in degrading high-density polyethylene.

6. The use according to claim 5, characterized in that The degradation is that the Bosella QY2 strain grows with the high-density polyethylene as the only carbon source and gradually degrades the high-density polyethylene.

7. The use according to claim 5, characterized in that The high-density polyethylene is high-density polyethylene particles, films or powders.

8. The use according to claim 5, characterized in that The high-density polyethylene was degraded by the Bosella QY2 strain in different water bodies.

9. A method for degrading high-density polyethylene, characterized in that: The Bosella QY2 strain according to claim 1 or the bacterial agent according to any one of claims 2 to 4 is co-cultured with high-density polyethylene.

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