一种稻黄单胞菌抗体检测试剂盒及其应用
By optimizing the preparation process of Xanthomonas oryzae XadA antigen and developing a highly specific and sensitive monoclonal antibody 2A5, the problems of rapid and accurate detection of Xanthomonas oryzae have been solved, enabling efficient qualitative and quantitative detection of Xanthomonas oryzae and supporting early disease diagnosis and control.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SHANGHAI LINGEN BIOTECHNOLOGY CO LTD
- Filing Date
- 2024-12-31
- Publication Date
- 2026-07-17
AI Technical Summary
Existing methods for detecting Xanthomonas oryzae are time-consuming and complex to operate. Furthermore, the development of monoclonal antibodies against Xanthomonas oryzae outer membrane proteins faces challenges such as structural complexity and low immunogenicity, making it difficult to achieve rapid and accurate detection.
The preparation process of XadA antigen from Xanthomonas oryzae was optimized, and combined with high-throughput screening technology, a highly specific and sensitive monoclonal antibody 2A5 was developed. Its application in a detection kit was designed, and qualitative and quantitative detection was achieved by binding with Xanthomonas oryzae-specific antigens with high affinity.
Rapid and accurate detection of Xanthomonas oryzae was achieved. The monoclonal antibody 2A5 has a detection limit of 0.5 ng/mL for XadA antigen, exhibiting good sensitivity and specificity, and supporting early disease diagnosis and control.
Smart Images
Figure CN119881307B_ABST