Application of hydrogen molecules in relieving sleep disorder and palpitation caused by coffee beverage

Abstract

The invention provides application of hydrogen molecules in relieving sleep disorder and / or palpitation caused by coffee drinks and application of a composition in relieving sleep disorder and / or palpitation, and the composition comprises hydrogen molecules and caffeine. Hydrogen molecules can relieve the sleep disorder problem caused by coffee drinks, the palpitation problem caused by coffee drinks, and the sleep disorder and the palpitation problem caused by coffee drinks.

Description

Technical Field

[0001] The present invention relates to the field of food, and in particular to the alleviation of sleep disorders and / or palpitations caused by coffee. Background Art

[0002] Coffee, as a globally loved beverage, has won the favor of countless people with its unique aroma and refreshing effect. However, the intake of coffee may also bring a series of health problems, including addiction, calcium loss, sleep disorders and palpitations.

[0003] Some studies have shown that coffee drinks can affect sleep in many ways. For example, the intake of coffee drinks can interfere with the secretion of melatonin, leading to difficulty falling asleep; it can reduce deep sleep time, increase the number of awakenings at night, and reduce sleep quality; long-term intake of coffee drinks may also affect the biological clock and lead to sleep cycle disorders.

[0004] In order to alleviate the sleep disorders caused by coffee drinks, some researchers have further added L-theanine to coffee drinks. L-theanine is an amino acid found in tea leaves and has been shown to improve sleep quality. However, the combination of L-theanine and coffee drinks is not effective in alleviating the sleep disorders caused by coffee drinks, and the sweetness and umami taste of L-theanine will affect the taste of coffee drinks.

[0005] In addition, the intake of coffee drinks can stimulate the heart, causing an increase in heart rate and arrhythmia. In the hope of alleviating the palpitations caused by coffee drinks, current research uses active substances to add to coffee drinks, but the addition of these active substances is either ineffective or destroys the flavor of the coffee itself, and therefore is not suitable for addition to coffee drinks.

[0006] Therefore, there is a need to further alleviate the sleep disorders and / or palpitations caused by coffee beverages without destroying the original flavor of coffee. Summary of the invention

[0007] Throughout the specification, unless otherwise specifically stated, the terms used herein should be understood as meanings commonly used in the art. Therefore, unless otherwise defined, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art to which the present invention belongs. In the event of a conflict, the present specification takes precedence.

[0008] When studying the use of hydrogen molecules in making beverages, the inventors found that hydrogen molecules used in coffee beverages can alleviate some of the negative effects caused by coffee, namely sleep disorders and palpitations. This is quite unexpected. In fact, some researchers have previously studied the effects of hydrogen on blood pressure and heart rate by having subjects drink hydrogen-rich water, and concluded that there was no significant change in blood pressure and heart rate before and after the experiment. However, in the inventors' research, the use of hydrogen molecules surprisingly showed a significant improvement in palpitations caused by coffee beverages.

[0009] Accordingly, on the one hand, the present application provides the use of hydrogen molecules in alleviating sleep disorders caused by coffee beverages.

[0010] In addition, the present application provides the use of hydrogen molecules in alleviating the problem of palpitations caused by coffee beverages.

[0011] In addition, the present application provides the use of hydrogen molecules in alleviating sleep disorders and palpitations caused by coffee beverages.

[0012] In another aspect, the present application provides use of a composition for alleviating sleep disorders, wherein the composition comprises hydrogen molecules and caffeine.

[0013] In addition, the invention provides the use of the composition of the present application in relieving palpitations. The invention provides the use of the composition of the present application in improving heart rhythm and / or blood pressure.

[0014] In addition, the invention provides use of the composition of the present application in alleviating sleep disorders and palpitations.

[0015] In a specific embodiment, the sleep disturbance is caused by caffeine.

[0016] In a specific embodiment, the palpitations are caused by caffeine.

[0017] In specific embodiments, the disturbance in heart rhythm or abnormal blood pressure is caused by caffeine.

[0018] In some embodiments, the hydrogen molecules are present in a form that includes a gas.

[0019] In some embodiments, the hydrogen molecules exist in a form including being dissolved in a liquid.

[0020] In some embodiments, the hydrogen molecules are present in the form of dissolved in the coffee beverage.

[0021] Hydrogen molecules in gaseous form, i.e., hydrogen, can be prepared in various feasible ways. For example, it can be produced by electrolysis of water, physical separation from a mixed gas containing hydrogen, microbial fermentation, or by a hydrogen generator. Among them, "hydrogen generator" refers to a chemical substance that can produce hydrogen through a chemical reaction. These generators usually react with water to release hydrogen, for example, some metals or metal compounds, such as magnesium metal, calcium metal, or alloys or oxides of these metals.

[0022] Hydrogen molecules can be dissolved in liquids in various feasible ways, such as by electrolysis of water, physical mixing. In some embodiments, hydrogen molecules can be dissolved directly in coffee beverages. In some embodiments, hydrogen molecules can be dissolved in a medium base (e.g., water or milk, etc.) of coffee beverages.

[0023] In this article, "the base of coffee beverage" refers to the liquid component as the basis of coffee beverage. For example, in black coffee, the base of coffee beverage can be water. For another example, in latte, the base of coffee beverage can be milk.

[0024] In a specific embodiment, the hydrogen molecules are present in the composition of the present application in the form of a gas or dissolved in a liquid. In some embodiments, the hydrogen molecules are present in the composition of the present application in the form of a gas or dissolved in a liquid.

[0025] In some embodiments, when hydrogen molecules are dissolved in a coffee beverage, the concentration of hydrogen molecules is from about 0.5 ppm to about 10 ppm, for example, about 0.5 ppm, about 0.6 ppm, about 0.7 ppm, about 0.8 ppm, about 0.9 ppm, about 1.0 ppm, about 1.1 ppm, about 1.2 ppm, about 1.3 ppm, about 1.4 ppm, about 1.5 ppm, about 1.6 ppm, about 1.7 ppm, about 1.8 ppm, about 1.9 ppm, about 2.0 ppm, about 2.5 ppm, about 3.0 ppm, about 3.5 ppm, about 4.0 ppm, about 4.5 ppm, about 5.0 ppm, about 5.5 ppm, about 6.0 ppm, about 6.5 ppm, about 7.0 ppm, about 7.5 ppm, about 8.0 ppm, about 8.5 ppm, about 9.0 ppm, about 9.5 ppm, about 10.0 ppm.

[0026] In some specific embodiments, when hydrogen molecules are dissolved in the coffee beverage, the concentration of hydrogen molecules is 0.5 ppm to 7 ppm.

[0027] In some embodiments, where hydrogen molecules are dissolved in the coffee beverage, the concentration of hydrogen molecules is between 0.5 ppm and 6 ppm.

[0028] The amount of hydrogen molecules in coffee beverages can be measured by liquid chromatography.

[0029] In some embodiments, the ratio of the concentration of caffeine to the concentration of hydrogen molecules in the coffee beverage or composition is about 20:1 to about 3000:1, for example, about 20:1, about 50:1, about 100:1, about 150:1, about 200:1, about 250:1, about 300:1, about 350:1, about 400:1, about 450:1, about 500:1, about 550:1, about 600:1, about 700:1, about 800:1, about 900:1, about 1000:1, about 1100:1, about 1200:1, about 1300:1, about 1400:1, about 1500:1, about 1600:1, about 1700:1, about 1800:1, about 1900:1, about 2000:1, about 2000:1, about 2500:1, about 3000:1, about 3500:1, about 4000:1, about 4500:1, about 5000:1, about 5500:1, about 6000:1, about 6000:1, about 7 ... 1. About 650:1, about 700:1, about 750:1, about 800:1, about 850:1, about 900:1, about 950:1, about 1000:1, about 1200:1, about 1400:1, about 1600:1, about 1800:1, about 2000:1, about 2200:1, about 2400:1, about 2600:1, about 2800:1, about 3000:1.

[0030] In some embodiments, the coffee beverage or composition of the present application includes caffeine in a concentration of about 200 mg / kg to about 1800 mg / kg, such as about 200 mg / kg, about 300 mg / kg, about 400 mg / kg, about 500 mg / kg, about 600 mg / kg, about 700 mg / kg, about 800 mg / kg, about 900 mg / kg, about 1000 mg / kg, about 1100 mg / kg, about 1200 mg / kg, about 1300 mg / kg, about 1400 mg / kg, about 1500 mg / kg, about 1600 mg / kg, about 1700 mg / kg, and about 1800 mg / kg of caffeine.

[0031] In some embodiments, the caffeine concentration of the coffee beverage or composition of the present application is greater than or equal to 200 mg / kg, greater than or equal to 400 mg / kg, greater than or equal to 600 mg / kg, greater than or equal to 800 mg / kg, greater than or equal to 1000 mg / kg, or greater than or equal to 1500 mg / kg.

[0032] As used herein, the term "about" means within ±10% of the corresponding number or value.

[0033] As used herein, the term "caffeine" refers to an organic compound naturally present in coffee beans, tea leaves, cola nuts, and guarana leaves, etc., with a chemical formula of C8H 10 N4O2, belongs to the class of xanthine alkaloids.

[0034] In this article, the concentration of caffeine, when there is a drinking suggestion, can refer to the concentration when eating or drinking. For example, a net content of 33 ml of concentrated coffee liquid with a caffeine content of 3000 mg / kg is recommended to be added to 300 ml of water, milk or other beverages when drinking; in this case, the caffeine concentration is 297.3 mg / kg.

[0035] In a specific embodiment, the coffee beverage is made using one or more of Arabica coffee beans, Liberica coffee beans and Robusta coffee beans as raw materials.

[0036] There is no particular limitation on the origin of the coffee beans in this application.

[0037] There is no particular limitation on the coffee bean processing method in this application. For example, water washing, sun drying, semi-water washing, wet hulling, barrel fermentation, anaerobic fermentation, and honey processing can be used, but are not limited thereto.

[0038] There is no particular limitation on the roasting degree of the coffee beans, for example, light roasting, medium roasting or dark roasting.

[0039] The present application has no particular restrictions on the extraction method of coffee beans, for example, immersion extraction, decoction extraction, drip extraction, pressurized extraction (high pressure extraction), and percolation extraction, but not limited thereto.

[0040] The present application has no particular restrictions on the type of coffee beverage. The coffee beverage may be a solid beverage or a liquid beverage. The coffee beverage may be selected from one or more of the following groups: black coffee, American coffee, instant coffee, freeze-dried coffee, extracted coffee liquid or concentrated coffee liquid, capsule coffee, drip coffee, latte coffee, mocha coffee, oatmeal coffee, matcha coffee and other beverages as coffee base.

[0041] The coffee or composition of the present application may further include other ingredients suitable for making coffee-like beverages, and exemplary ingredients include: one or more of the group consisting of water, milk, syrup and non-dairy creamer. "Milk" can be, for example, liquid dairy products (such as whole milk, skim milk, coconut milk), milk powder products, fermented dairy products, cream products, cheese products, etc. "Syrup" can be, for example, honey, maple syrup, corn syrup or other flavored syrups. "Non-dairy creamer" is a powder composed of vegetable oil, corn syrup, emulsifiers, etc., which can be used to increase the milkiness and fineness of coffee. The coffee or composition of the present application may further include carbon dioxide molecules and / or nitrogen molecules in the form of being dissolved in a liquid; carbon dioxide molecules and / or nitrogen molecules can be introduced into the liquid in a physically mixed manner, for example. The coffee or composition of the present application may further include food additives, such as additives that comply with the national standard GB2760-2014, such as stabilizers, emulsifiers, food spices, flavor enhancers, sweeteners, preservatives, colorants, etc.) and the like.

[0042] In some embodiments, the composition of the present application is a food composition, a beverage composition or a pharmaceutical composition.

[0043] In a specific embodiment, the use of the composition of the present application in improving sleep is provided, and the composition is a health care product composition.

[0044] By using hydrogen molecules in combination with coffee drinks, on the one hand, it brings about a significant effect of relieving sleep disorders, while not causing damage to the flavor of the coffee drink; compared with other substances that relieve sleep disorders, it even brings a more significant effect of relieving sleep disorders. On the other hand, it brings a significant effect of relieving palpitations and adjusting heart rhythm, and hydrogen molecules do not cause damage to the flavor of the coffee drink; compared with the combination of other substances that relieve palpitations and coffee drinks, it even brings a more significant effect of relieving palpitations. In addition, the use of hydrogen molecules in combination with coffee drinks can simultaneously relieve sleep disorders and palpitations caused by coffee drinks. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] Figure 1 The results of the sleep latency, total sleep duration, non-rapid eye movement sleep and rapid eye movement sleep of each group of mice analyzed by electroencephalogram and electromyogram in Example 2 (T test; **: P < 0.01; ***: P < 0.001);

[0046] Figure 2 The results of the analysis of the caffeine concentration of the solutions of the experimental group 1 and the control group A1 after 24 hours and 48 hours of storage by liquid chromatography (T test; **: P < 0.01);

[0047] Figure 3The results of the latency, total sleep duration, non-rapid eye movement sleep and rapid eye movement sleep of each group of mice analyzed by electroencephalogram and electromyogram in Example 4 are shown in Figure 4 (T test; ***: P < 0.001);

[0048] Figure 4 The results of the latency, total sleep duration, non-rapid eye movement sleep and rapid eye movement sleep of each group of mice analyzed by electroencephalogram and electromyogram in Example 5 (T test; ***: P < 0.001);

[0049] Figure 5 The results of the heart rate change rate and the number of premature beats of each group of mice analyzed by the small animal-specific non-invasive ECG telemetry system in Example 6 (T test; ***: P < 0.001);

[0050] Figure 6 This is a result diagram of the heart rate change rate and number of premature beats of each group of mice analyzed using the small animal-specific non-invasive ECG telemetry system in Example 7;

[0051] Figure 7 The results of the analysis of the heart rate change rate and the number of premature beats of each group of mice using the non-invasive ECG telemetry system for small animals in Example 8 are shown in Figure 8 (T test; ***: P < 0.001). DETAILED DESCRIPTION

[0052] The present invention will be described in detail below in conjunction with specific implementations and examples, and the advantages and various effects of the present invention will be more clearly presented. It should be understood by those skilled in the art that these specific implementations and examples are used to illustrate the present invention, rather than to limit the present invention.

[0053] Example 1 Preparation of coffee

[0054] First, supersaturated hydrogen water was prepared using nanobubbles, and commercially available coffee concentrate was prepared. Experimental Group 1 was a coffee composition with a final caffeine concentration of 1500 mg / kg and a final hydrogen concentration of 2 ppm obtained by diluting coffee concentrate with the prepared supersaturated hydrogen water. At the same time, a control group was set up, in which control group A1 was a coffee solution with a final caffeine concentration of 1500 mg / kg obtained by diluting coffee concentrate with pure water; control group B was a coffee composition containing no hydrogen but containing 1500 mg / kg caffeine and 140 g / L L-theanine solution obtained by dissolving L-theanine in pure water and diluting coffee concentrate. As shown in Table 1 below, L-theanine can inhibit the excessive release of excitatory neurotransmitters such as glutamate and promote the secretion of inhibitory neurotransmitters such as γ-aminobutyric acid (GABA). It is a recognized substance that can relieve sleep disorders; in addition, it is reported that L-theanine can improve the anxiety of the subjects, and also show a stable heart state in some electrocardiogram indicators, which has a certain positive effect on palpitations.

[0055] Table 1

[0056]

[0057] Example 2 Testing of sleep disorder relief effect

[0058] Animal experiment steps: Select healthy male C57BL / 6 mice aged 6-8 weeks with a body weight of 18-22g, and raise them in an environment with a temperature of (22±2)℃, a relative humidity of (50±10)%, and 12 hours of light / 12 hours of darkness, and feed and drink freely. Prepare each group of solutions as described in Example 1, and randomly divide the mice into 4 groups, namely, a normal control group, an experimental group 1, a control group A1, and a control group B, with 5 mice in each group, and feed them with ordinary feed every day. According to previous reports, when mice use caffeine greater than 40mg / kg according to their body weight, it can cause obvious sleep disorders. Therefore, each group of solutions is gavaged at 0.3mL / 10g body weight, so that the caffeine dosage of mice is 45mg / kg / day, and the L-theanine dosage of mice in the control group B is 200mg / kg / day, wherein the normal control group is gavaged with ordinary drinking water at 0.3mL / 10g body weight. The mice's sleep was recorded using an electroencephalogram (EEG) and electromyogram (EMG) monitoring system every day to analyze the sleep latency (the time required from preparing to fall asleep to entering the sleep state), total sleep duration, non-rapid eye movement sleep (NREM) and rapid eye movement sleep (REM) time and proportion, etc. This lasted for 2 weeks. The results showed that Figure 1 middle.

[0059] Depend on Figure 1 It can be seen that the mice in the normal control group had no sleep disorder, moderate autonomous activity, short sleep latency, long sleep duration, and normal NREM and REM sleep ratio. In the control group A1 (coffee group only), it can be observed that the mice have a prolonged sleep latency, reduced total sleep time, fragmented sleep, and disordered sleep structure, indicating that sleep disorders are successfully induced. In the control group B (L-theanine + coffee group), the sleep latency was shortened to a certain extent compared with the control group A1, the total sleep time increased, and the NREM and REM sleep ratios also improved, indicating that L-theanine improved coffee-induced sleep disorders to a certain extent. In the experimental group 1 (hydrogen + coffee group), not only was the sleep latency significantly shortened compared with the control group A1 (coffee group only), the total sleep time increased, and the NREM and REM sleep ratios were significantly improved, proving that hydrogen can significantly improve coffee-induced sleep disorders; further, the improvement effect of the experimental group 1 was statistically significantly better than that of the control group B (L-theanine + coffee group).

[0060] Example 3 Investigating the effect of hydrogen molecules on the caffeine concentration in beverages

[0061] In order to verify whether hydrogen can improve sleep disorders by reducing the caffeine content in the solution, the solution of experimental group 1 was stored for 24 hours and 48 hours, and the concentration of caffeine in the solution was detected by high performance liquid chromatography (HPLC). The results are as follows: Figure 2 The results showed that compared with the control group A1 (coffee group only), the caffeine concentration in the experimental group 1 solution did not decrease significantly after 24h and 48h of storage, so the effect of hydrogen on improving sleep disorders caused by caffeine is not achieved by reducing the caffeine content.

[0062] Example 4 Testing the effects of different concentrations of hydrogen molecules on relieving coffee-induced sleep disorders

[0063] Supersaturated hydrogen water prepared by nanobubbles, pure water and commercially available coffee concentrate were mixed and diluted in different proportions to obtain hydrogen-containing coffee solutions (experimental groups 2-7) with a final caffeine concentration of 1500 mg / kg and final hydrogen concentrations of 0.5 ppm, 1 ppm, 1.6 ppm, 4 ppm, 6 ppm and 7 ppm, as shown in Table 2. According to the animal experimental method in Example 2, mice were randomly divided into 6 groups, 5 mice in each group, and the corresponding solutions of experimental groups 2-7 were gavaged at 0.3 mL / 10 g body weight. The electroencephalogram (EEG) and electromyogram (EMG) monitoring systems were used regularly every day to record the sleep status of the mice, and the sleep latency, total sleep duration, non-rapid eye movement sleep (NREM) and rapid eye movement sleep (REM) time and proportion, etc. were analyzed for a total of 2 weeks.

[0064] Table 2

[0065]

[0066] The experimental results are as follows Figure 3 As shown, the results showed that similar to the effect of experimental group 1, the sleep latency of mice in experimental groups 2-6 (hydrogen 0.5ppm-6ppm) was significantly shortened compared with the control group A1 (coffee only group). The total sleep time was significantly increased, and the NREM and REM sleep ratios were significantly improved. The sleep latency of mice in experimental group 7 (hydrogen 7ppm) was slightly shortened, the total sleep time was slightly increased, and the NREM and REM sleep ratios were improved, but the effect was weaker than that of experimental groups 2-6.

[0067] The above results show that hydrogen final concentrations of 0.5-6ppm can improve sleep disorders caused by high concentrations of caffeine; while excessively high hydrogen concentrations, such as a final hydrogen concentration of 7ppm, weaken the improvement effect.

[0068] Example 5 Testing of the effect of hydrogen molecules on relieving sleep disorders under different caffeine concentrations and different substrate conditions

[0069] Supersaturated hydrogen water prepared by nanobubbles, pure water, commercially available whole milk powder and coffee concentrate were mixed and diluted in different proportions to prepare: a hydrogen-containing coffee solution with a final caffeine concentration of 2000 mg / kg and a final hydrogen concentration of 2 ppm (experimental group 8), a coffee solution containing only 2000 mg / kg caffeine without hydrogen (control group A2), a hydrogen-containing coffee solution with a final caffeine concentration of 1000 mg / kg and a final hydrogen concentration of 2 ppm (experimental group 9), a coffee solution containing only 1000 mg / kg caffeine without hydrogen (control group A3), a hydrogen-containing milk coffee solution containing whole milk with a final caffeine concentration of 1500 mg / kg and a final hydrogen concentration of 2 ppm (experimental group 10), and a milk coffee solution containing no hydrogen but whole milk and 1500 mg / kg caffeine (control group A4), as shown in Table 3. According to the animal experiment method in Example 2, mice were randomly divided into 6 groups, 5 mice in each group, and the corresponding solutions of the above groups were gavaged at 0.3 mL / 10 g body weight. The electroencephalogram (EEG) and electromyogram (EMG) monitoring systems were used to record the sleep status of the mice at regular intervals every day, and the sleep latency, total sleep time, non-rapid eye movement sleep (NREM) and rapid eye movement sleep (REM) time and proportion were analyzed for a total of 2 weeks.

[0070] Table 3

[0071]

[0072] The test results are as follows Figure 4 As shown, the results showed that the mice in the control group A2 had a longer sleep latency, less total sleep time, fragmented sleep, and disordered sleep structure, indicating a higher degree of sleep disorder. Compared with the control group A2, the sleep latency of mice in experimental group 8 was significantly shortened, the total sleep time increased, and the proportion of NREM and REM sleep was significantly improved. The mice in the control group A3 also had a longer sleep latency, less total sleep time, fragmented sleep, and a higher degree of sleep disorder, but the overall degree was lower than that of the control group A2. Experimental group 9 also showed similar effects to experimental group 1, with a shortened sleep latency, increased total sleep time, and improved NREM and REM sleep ratios. Experimental group 10 and control group A4 showed similar effects to experimental group 1 and control group A1. Compared with control group A4, the sleep disorder of control group 10 was significantly improved. The above results prove that hydrogen can improve sleep disorders caused by caffeine, whether it is high concentration of caffeine, low concentration of caffeine or other substrate conditions.

[0073] Example 6 Test of palpitation relief effect

[0074] Animal experiment steps: Select healthy male C57BL / 6 mice aged 6-8 weeks with a body weight of 18-22g, and raise them in an environment with a temperature of (22±2)℃, a relative humidity of (50±10)%, and 12 hours of light / 12 hours of dark alternation, and feed and drink water freely. According to the animal experiment in Example 1, each group of solutions was gavaged at 0.3mL / 10g body weight, and the electrocardiogram parameters of each group of mice in a stable state before gavage and within 1h after gavage, i.e., heart rate and electrocardiogram, were recorded using a non-invasive electrocardiogram telemetry system for small animals. Calculate the heart rate change rate: (heart rate after gavage-heart rate before gavage) / heart rate before gavage × 100%; by observing the electrocardiogram, count the average number of heartbeats that appear in advance outside the normal heartbeat rhythm within 1min, i.e., the number of premature beats, which is one of the important indicators for judging arrhythmia. The larger the heart rate change rate and the number of premature beats, the higher the degree of palpitations.

[0075] The test results are as follows Figure 5 As shown, the results showed that the heart rate of mice in the normal control group was relatively stable before and after gavage, with small fluctuations, and no arrhythmia. The heart rate of the control group A1 was significantly accelerated, with a heart rate change rate of up to 45% and an average number of premature beats of 17 times, indicating that caffeine caused palpitations. The heart rate change rate of experimental group 1 was 11%, and the average number of premature beats was 4 times. Compared with the control group A1, experimental group 1 significantly improved the degree of palpitations. The heart rate change rate of control group B was 21%, and the average number of premature beats was 8 times. Compared with the control group A1, control group B improved the caffeine-induced palpitations to a certain extent. The improvement effect of experimental group 1 was significantly better than that of control group B. The above results show that both hydrogen and L-theanine can relieve caffeine-induced palpitations, and the relief effect of hydrogen is significantly better than that of L-theanine.

[0076] Example 7 Test of the effect of relieving palpitations under different concentrations of hydrogen molecules

[0077] According to the animal experiment method described in Example 6, the solutions in experimental groups 2-7 were used for intragastric administration and the electrocardiographic parameters of mice were detected. The test results are shown in Figure 6 As shown, the results show that compared with the control group A1, the heart rate change rate and average number of premature beats in experimental groups 2-6 were significantly reduced, and the degree of reduction was basically inversely proportional to the hydrogen concentration. In other words, experimental groups 2-6 also showed similar effects to experimental group 1, indicating that the final concentration of hydrogen of 0.5-6ppm also has a significant improvement effect on caffeine-induced palpitations. When the hydrogen concentration reached 7ppm (experimental group 7), the degree of improvement in heart rate was weakened.

[0078] Example 8 Test on the effect of hydrogen molecules in relieving palpitations under different caffeine concentrations and different substrate conditions

[0079] According to the animal experiment described in Example 6, the solutions in experimental groups 8-10 and control groups A2-A4 were used for intragastric administration and the electrocardiogram parameters of mice were detected. The test results are shown in Figure 7 As shown, the results showed that the heart rate change rate of mice in the control group A2 was as high as 62%, and the average number of premature beats was 28 times, indicating that high concentrations of caffeine caused a higher degree of palpitations than the control group A1. The heart rate change rate of mice in experimental group 8 was 23%, and the average number of premature beats was 9 times. Compared with the control group A2, experimental group 8 significantly improved the degree of palpitations. The heart rate change rate of mice in the control group A3 was 18%, and the average number of premature beats was 7 times, indicating that low concentrations of caffeine caused a lower degree of palpitations. The heart rate change rate of mice in experimental group 9 was 10%, and the average number of premature beats was 2 times. Compared with the control group A3, the degree of palpitations in experimental group 9 was also significantly improved. For control group A4 and experimental group 10, similar to control group A1 and experimental group 1, the heart rate change rate of mice in control group A4 was 46%, and the average number of premature beats was 17 times; the heart rate change rate of experimental group 10 was 12%, and the average number of premature beats was 4 times. Compared with the control group A4, the degree of palpitations in experimental group 10 was also significantly improved.

[0080] The above results prove that hydrogen can significantly relieve caffeine-induced palpitations under high caffeine concentration, low caffeine concentration or other base conditions.

Claims

1. Use of hydrogen molecules in alleviating sleep disorders and / or palpitations caused by coffee beverages.

2. The use according to claim 1, wherein In the case where hydrogen molecules are dissolved in the coffee beverage, the concentration of the hydrogen molecules is 0.5 ppm to 10 ppm, such as 0.5 ppm to 7 ppm.

3. The use according to claim 1, wherein In the case where hydrogen molecules are dissolved in the coffee beverage, the concentration of the hydrogen molecules is 0.5 ppm to 6 ppm.

4. The use according to claim 2, wherein The ratio of the concentration of caffeine in the coffee beverage to the concentration of the hydrogen molecules is 20:1 to 3000:

1.

5. The use according to claim 1, wherein The hydrogen molecules exist in a gaseous form or in a form dissolved in a liquid. For example, the hydrogen molecules exist in a form dissolved in a coffee beverage.

6. The use according to any one of claims 1 to 5, wherein The caffeine concentration in the coffee beverage is greater than or equal to 200 mg / kg, such as greater than or equal to 800 mg / kg.

7. The use according to any one of claims 1 to 6, wherein The coffee beverage is prepared by using one or more of Arabica coffee beans, Liberica coffee beans and Robusta coffee beans as raw materials.

8. The use according to any one of claims 1 to 7, wherein The coffee beverage further comprises one or more of water, milk, syrup and non-dairy creamer.

9. The use according to any one of claims 1 to 8, wherein The coffee beverage is selected from one or more of the group consisting of: black coffee, American coffee, instant coffee, freeze-dried coffee, extracted coffee liquid or concentrated coffee liquid, capsule coffee, drip coffee, latte coffee, mocha coffee, oatmeal coffee, matcha coffee and other coffee beverages as the base.

10. Use of a composition for relieving sleep disorders and / or palpitations, wherein: The composition includes molecular hydrogen and caffeine.

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