Rose essential oil liposome prepared by taking calamine as carrier as well as preparation method and application of rose essential oil liposome

Abstract

The invention relates to the technical field of flavors and fragrances, in particular to a rose essential oil liposome prepared by taking calamine as a carrier as well as a preparation method and application of the rose essential oil liposome. In order to solve the problems that an existing liposome preparation process is tedious, the production cost is too high, the dosage of an organic solvent is large, and large-scale preparation is difficult, calamine and a wrapping material are combined together to prepare a no-load liposome, and the no-load liposome is coated with rose essential oil to prepare a rose essential oil liposome suspension. The combination process of the calamine and the wrapping material is mild in condition, special equipment is not needed, the biological activity of active ingredients is not influenced in the drug wrapping process, the pH value of a solution is not changed, no solvent is left, and a novel preparation process of the rose essential oil liposome, which is simple in process, small in organic solvent dosage, high in encapsulation efficiency and high in stability, is constructed.

Description

Technical Field

[0001] The invention relates to the technical field of essences and fragrances, and in particular to a rose essential oil liposome prepared by taking calamine as a carrier, a preparation method and application thereof. Background Art

[0002] Rose essential oil can be obtained from fresh roses by steam distillation. The oil yield is generally 0.02% to 0.04%. The main components include linalool, geraniol, etc. Due to its unique aroma and multiple medicinal values, it is widely used in food, medicine, cosmetics and fragrance industries. Studies have shown that rose essential oil has multiple biological activities such as antibacterial and bactericidal, anti-anxiety, antioxidant, anti-inflammatory, anti-aging, sleep-inducing, blood circulation and blood stasis, and skin moisturizing. However, rose essential oil also has the disadvantages of being volatile, unstable and difficult to preserve, which to a certain extent limits the application of rose essential oil in food and cosmetics.

[0003] Liposome technology can encapsulate rose essential oil in liposomes, improve its stability, and improve the solubility of essential oil in water. Liposomes are mainly composed of orderly arranged phospholipid bilayers, forming spheres with closed vesicle structures, with a diameter in the range of 25-1000nm. Liposomes can encapsulate active substances such as rose essential oil fragrance components to form nanoparticles. The closed environment weakens the volatilization of rose essential oil and has a slow and controlled release effect. There is currently little research on the preparation of rose essential oil liposomes. Existing liposome preparations mostly use organic solvent injection method, thin film dispersion method, reverse evaporation method, etc. Chen Cheng et al. (Chen Cheng, Yan Mengru, Xue Shouyu, et al. Preparation and characterization of peony seed oil liposomes [J]. China Food Additives, 2024, 35(3): 19-24.) used the ethanol injection-ultrasonic method to prepare peony seed oil liposomes, with an encapsulation efficiency of 87.16%, a particle size of 143.6nm, a PDI of 0.217, and a Zeta potential of -48.3mV. The obtained liposomes had a high encapsulation rate and good stability; Ma Dan et al. (Ma Dan, Shang Shuyou, Li Wenqi, et al. Research on the preparation process of dendrobium flower volatile oil liposomes [J]. Agricultural Products Processing, 2023(24): 42-45.) used a thin film dispersion method to prepare dendrobium flower volatile oil liposomes. The obtained liposomes had a good shape and the optimal encapsulation rate was 56.45%.

[0004] However, the existing liposome preparation process still has certain defects, such as the preparation process is too complicated, the production cost is too high, a large amount of organic solvents are used, and the stability is not ideal. Summary of the invention

[0005] The invention aims at the problems that the existing liposome preparation process is cumbersome, the production cost is too high, the amount of organic solvent is large, and it is difficult to prepare on a large scale. The present invention combines calamine with a packaging material to prepare an empty liposome, and coats rose essential oil to prepare rose essential oil liposome. The process of combining calamine with the packaging material is mild and does not require special equipment. The drug coating process does not affect the biological activity of the active ingredient, does not change the pH value of the solution, and has no solvent residue. The present invention constructs a new process for preparing rose essential oil nanoliposomes with simple process, low organic solvent consumption, high encapsulation rate, and high stability, which provides a new idea for the large-scale preparation of rose essential oil liposomes and has significant application value.

[0006] In order to solve the above problems, the present invention adopts the following technical scheme, a method for preparing rose essential oil liposomes using calamine as a carrier, comprising the following steps:

[0007] (a) adding a wrapping material into an anhydrous ethanol solution to prepare a wrapping material solution;

[0008] (b) immersing the solid phase particles in a coating material solution, removing the anhydrous ethanol, and combining the solid phase particles with the coating material to obtain empty liposomes;

[0009] (c) mixing the empty liposomes with the rose essential oil aqueous dispersion to obtain a rose essential oil liposome suspension.

[0010] The encapsulating material is one of lecithin and lecithin-cholesterol;

[0011] In the lecithin-cholesterol, the mass ratio of lecithin to cholesterol is (4-8):1, preferably 6:1.

[0012] The solid phase particles are calamine. The mechanism of action of the solid phase particles is to utilize the excellent specific surface area of ​​the calamine particles to make the coating material solution volatilize on the surface of the calamine particles to form a film.

[0013] The method for removing the anhydrous ethanol is one of evaporating in a high-temperature water bath, blowing dry, and standing at room temperature.

[0014] In step (c), the empty liposomes and the rose essential oil aqueous dispersion are mixed in a manner including: vortex oscillation, shaking, and ultrasound.

[0015] The mass concentration of the encapsulation material in the encapsulation solution is 0.04726 to 0.2 g / mL;

[0016] The mass ratio of the calamine solid phase particles to the encapsulating material is (1.786-4.167):1, preferably (3.12-4.167):1.

[0017] The ratio of the volume of rose essential oil to the mass of lecithin is (0.028-0.05) mL / g, preferably (0.028-0.04) mL / g;

[0018] The solvent of the rose essential oil water dispersion is water, and the volume fraction of the rose essential oil water dispersion is 0.025-0.1%.

[0019] The encapsulation rate of the rose essential oil liposome prepared by the preparation method is 44.9-96.9%.

[0020] Furthermore, the encapsulation efficiency of the rose essential oil liposomes was measured by HPLC-centrifugation method;

[0021] The centrifugal speed is 6000-12000 rpm, and the centrifugal time is 10-20 min.

[0022] The present invention also provides an application of the rose essential oil liposome prepared by the preparation method in preparing a skin external preparation.

[0023] The dosage form of the rose essential oil liposome is a suspension or a semisolid, preferably an ointment.

[0024] The present invention also provides an application of the rose essential oil liposome prepared by the preparation method in preparing solid spices.

[0025] The dosage form of the rose essential oil liposome is a solid dosage form, preferably a powder.

[0026] Beneficial Effects

[0027] 1. The present invention combines a wrapping material on the surface of calamine solid-phase particles for the first time to form empty liposomes, and embeds rose essential oil in the empty liposomes, thereby constructing a new process for preparing liposomes with simple process, small amount of organic solvent and fast. The calamine solid-phase particles used in the present invention have large specific surface area, thus being easier to form a thin and uniform film on the surface.

[0028] 2. The present invention adopts high-density calamine solid phase particles as carriers. During the drying process, only a short-time, low-speed centrifugal operation is required to completely separate the rose essential oil liposomes from the dispersed system, which greatly reduces the risk of instability of the rose essential oil liposomes.

[0029] 3. The present invention adds an aqueous solution of rose essential oil to empty liposomes to prepare rose essential oil liposomes, without the need for conventional steam extraction or dissolving the rose essential oil in an organic solvent, thereby avoiding the influence of heating on the rose essential oil when removing the organic solvent.

[0030] 4. The present invention adopts calamine solid phase particles with a large specific surface area as a carrier, which greatly improves the utilization rate of the packaging material, does not require a large amount of packaging material, and further does not require a large amount of organic solvent for dissolution, thereby reducing costs and shortening preparation time. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 HPLC spectra of the free essential oil and the encapsulated essential oil test solution of the rose essential oil liposomes of Examples 1-3;

[0032] Figure 2 HPLC spectra of the free essential oil and the encapsulated essential oil test solution of the rose essential oil liposome of Example 4-5;

[0033] Figure 3 HPLC spectra of the rose essential oil liposome free essential oil and the essential oil encapsulated test solution of Examples 6-9;

[0034] Figure 4 is a microscopic image (20X) of the empty liposome aqueous dispersion in Example 1;

[0035] Figure 5 This is a microscope image (20X) of the rose essential oil liposome aqueous suspension in Example 1. DETAILED DESCRIPTION

[0036] The present invention will be further described in detail below through specific implementation examples, but it should not be understood that the present invention is limited to the following examples, and all technologies realized based on the above content of the present invention belong to the scope of the present invention.

[0037] The lecithin described in the examples was purchased from Beijing Aoboxing Biological Co., Ltd.; rose essential oil was provided by Jinan Rose Garden Brewing Co., Ltd.; artificial skin was Strat-MTM Membrane purchased from Merck Millipore Ltd; cholesterol was purchased from Sinopharm Chemical Reagent Co., Ltd., batch number: 20191008; anhydrous ethanol was purchased from Tianjin Fuyu Fine Chemical Co., Ltd.; calamine was purchased from Tianjin Zhonglian Reagent Co., Ltd.

[0038] Embodiment 1:

[0039] Weigh lecithin and add anhydrous ethanol solution, prepare 0.2g / mL lecithin solution, ultrasonicate for 10 minutes, and store under light-proof conditions. The lecithin solution is placed in an evaporating dish, heated in a water bath at 50°C, and then calamine is added according to the mass ratio of calamine solid phase particles to lecithin 4.167:1, stirred while adding, evaporated in a water bath, and empty liposomes are obtained. Take empty liposomes, add a volume fraction of 0.025% of rose essential oil aqueous dispersion according to the ratio of 0.028mL / g of rose essential oil volume and lecithin mass, shake for 10min, and obtain rose essential oil liposome suspension.

[0040] Embodiment 2:

[0041] Weigh lecithin and add anhydrous ethanol solution, prepare 0.2g / mL lecithin solution, ultrasonicate for 10 minutes, and store under light-proof conditions. Place the lecithin solution in an evaporating dish, heat it in a water bath at 50°C, then add calamine at a mass ratio of 3.12:1 of calamine solid phase particles and lecithin, stir while adding, evaporate in a water bath, and obtain empty liposomes. Take empty liposomes, add 0.05% of rose essential oil aqueous dispersion according to the ratio of 0.028mL / g of rose essential oil volume and lecithin mass, shake for 10min, and obtain rose essential oil liposome suspension.

[0042] Embodiment 3:

[0043] Weigh lecithin and add anhydrous ethanol solution, prepare 0.2g / mL lecithin solution, ultrasonicate for 10 minutes, and store under light-proof conditions. Place the lecithin solution in an evaporating dish, heat it in a water bath at 50°C, then add calamine at a mass ratio of 1.786:1 of silica gel solid phase particles and lecithin, stir while adding, evaporate in a water bath to obtain empty liposomes. Take empty liposomes, add 0.1% of rose essential oil aqueous dispersion according to the ratio of 0.028mL / g of rose essential oil volume and lecithin mass, shake for 10min, and obtain rose essential oil liposome suspension.

[0044] Embodiment 4:

[0045] Weigh lecithin and add anhydrous ethanol solution, prepare 0.2g / mL lecithin solution, ultrasonicate for 10 minutes, and store under light-proof conditions.Place lecithin solution in an evaporating dish, heat in a water bath at 50°C, then add calamine in a mass ratio of 1.786:1 of calamine solid phase particles and lecithin, stir while adding, evaporate in a water bath, and obtain empty liposomes.Take empty liposomes, add 0.025% of rose essential oil aqueous dispersion by volume according to the ratio of 0.04mL / g of rose essential oil volume and lecithin mass, shake for 10min, and obtain rose essential oil liposome suspension.

[0046] Embodiment 5:

[0047] Weigh lecithin and add anhydrous ethanol solution, prepare 0.2g / mL lecithin solution, ultrasonicate for 10 minutes, and store under light-proof conditions. The lecithin solution is placed in an evaporating dish, heated in a water bath at 50°C, and then calamine is added according to the mass ratio of calamine solid phase particles to lecithin 4.167:1, stirred while adding, evaporated in a water bath, and empty liposomes are obtained. Take empty liposomes, add a volume fraction of 0.05% of rose essential oil aqueous dispersion according to the ratio of 0.04mL / g of rose essential oil volume and lecithin mass, shake for 10min, and obtain rose essential oil liposome suspension.

[0048] Embodiment 6:

[0049] Weigh lecithin and add anhydrous ethanol solution, prepare 0.2g / mL lecithin solution, ultrasonicate for 10 minutes, and store under light-proof conditions. Place the lecithin solution in an evaporating dish, heat it in a water bath at 50°C, then add calamine at a mass ratio of 3.12:1 of calamine solid phase particles and lecithin, stir while adding, evaporate in a water bath, and obtain empty liposomes. Take empty liposomes, add 0.1% of rose essential oil aqueous dispersion by volume according to the ratio of 0.04mL / g of rose essential oil volume and lecithin mass, shake for 10min, and obtain rose essential oil liposome suspension.

[0050] Embodiment 7:

[0051] Weigh lecithin and add anhydrous ethanol solution, prepare 0.2g / mL lecithin solution, ultrasonicate for 10 minutes, and store under light-proof conditions. Place the lecithin solution in an evaporating dish, heat it in a water bath at 50°C, then add calamine at a mass ratio of 3.12:1 of calamine solid phase particles and lecithin, stir while adding, evaporate in a water bath, and obtain empty liposomes. Take empty liposomes, add 0.025% of rose essential oil aqueous dispersion according to the ratio of 0.05mL / g of rose essential oil volume and lecithin mass, shake for 10min, and obtain rose essential oil liposome suspension.

[0052] Embodiment 8:

[0053] Weigh lecithin and add anhydrous ethanol solution, prepare 0.04726g / mL lecithin solution, ultrasonicate for 10 minutes, and store under light-proof conditions. The lecithin solution is placed in an evaporating dish, heated in a water bath at 50°C, and then calamine is added in a mass ratio of calamine solid phase particles to lecithin 1.786:1, stirred while adding, evaporated in a water bath, and empty liposomes are obtained. Take empty liposomes, add a volume fraction of 0.05% of rose essential oil aqueous dispersion according to the ratio of 0.05mL / g of rose essential oil volume and lecithin mass, shake for 10min, and obtain rose essential oil liposome suspension.

[0054] Embodiment 9:

[0055] Weigh lecithin and add anhydrous ethanol solution, prepare 0.2g / mL lecithin solution, ultrasonicate for 10 minutes, and store under light-proof conditions.Place lecithin solution in an evaporating dish, heat in a water bath at 50°C, then add calamine in a mass ratio of 4.167:1 of calamine solid phase particles and lecithin, stir while adding, evaporate in a water bath, and obtain empty liposomes.Take empty liposomes, add 0.1% of rose essential oil aqueous dispersion by volume according to the ratio of 0.05mL / g of rose essential oil volume and lecithin mass, shake for 10min, and obtain rose essential oil liposome suspension.

[0056] Comparative Example 1

[0057] The preparation conditions of this comparative example are the same as those of Example 1, except that no calamine solid phase particles are added.

[0058] Weigh lecithin and add anhydrous ethanol solution to prepare 0.2g / mL lecithin solution, ultrasonicate for 10 minutes, and store in dark conditions. Add lecithin solution to a rotary evaporator, evaporate at 50°C to obtain lecithin film, add 0.025% rose essential oil aqueous dispersion according to the ratio of rose essential oil volume to lecithin mass of 0.028mL / g, ultrasonicate for 10min, and obtain rose essential oil liposome emulsion.

[0059] Comparative Example 2:

[0060] The preparation conditions of this comparative example are the same as those of Example 1, except that the calamine solid phase particles are replaced by montmorillonite of the same particle size.

[0061] Comparative Example 3:

[0062] The preparation conditions of this comparative example are the same as those of Example 1, except that the calamine solid phase particles are replaced with TiO 2 .

[0063] Comparative Example 4:

[0064] The preparation conditions of this comparative example are the same as those of Example 1, except that the calamine solid phase particles are replaced with silica gel of the same particle size.

[0065] Test example 1: Encapsulation efficiency test

[0066] Place 0.1 mL of rose essential oil liposome suspension in a common centrifuge tube, centrifuge at 8000 rpm for 10 min, take out the supernatant, filter it through a 0.45 μm filter membrane, and use it as the free rose essential oil test solution. Take the centrifugal sediment of common centrifugation and add anhydrous ethanol to the original solution volume, ultrasonicate for 5 min, centrifuge at 6000 rpm for 10 min, take out the supernatant, filter it through a 0.45 μm filter membrane, and use it as the encapsulated rose essential oil test solution for liquid chromatography analysis. Calculate the encapsulation efficiency according to the following formula:

[0067] Encapsulation efficiency (100%) = peak area of ​​linalool in encapsulated rose essential oil / (peak area of ​​linalool in free rose essential oil + peak area of ​​linalool in encapsulated rose essential oil) × 100%.

[0068] Since silica gel solid phase particles are not used as carriers in Comparative Example 1, effective data cannot be obtained by using the above encapsulation rate detection method. Therefore, the rose essential oil liposome encapsulation rate detection method prepared in Comparative Example 1 is as follows: 0.1mL rose essential oil liposome suspension is taken in a centrifuge tube, 8000rpm ordinary centrifugation for 10min, the supernatant is taken out and added to an ultrafiltration centrifuge tube, 0.3mL pure water is added, and the first ultrafiltration centrifugation is performed after 10min (wherein, the ultrafiltration membrane specification is 30KD, and the centrifugal speed is 8000rpm), and the permeate of the first ultrafiltration centrifugation is taken as the test solution for free rose essential oil detection. Take the centrifugal sediment of ordinary centrifugation, add 0.4mL anhydrous ethanol, ultrasonically dissolve, add to the ultrafiltration centrifuge tube of the first ultrafiltration centrifugation, 8000rpm ultrafiltration centrifugation for 10min, take the permeate to supplement the weight lost with anhydrous ethanol, as the test solution for the detection of encapsulated rose essential oil. The encapsulation rate is calculated to be 46.33%.

[0069] Chromatographic conditions: bonded silica gel C18 (4.6 mm*150 mm, 5 μm), 0.2% phosphoric acid water (A)-acetonitrile (B) as mobile phase, gradient elution (0-30 min, B 35%-80%; 30-35 min, B 100%; 35-40 min, B 35%), flow rate 1.0 mL / min, column temperature 30°C, detection wavelength 203 nm, injection volume: 20 μL.

[0070] Table 1 Rose essential oil liposome encapsulation efficiency (%)

[0071]

[0072]

[0073] Table 2 Range Analysis Table

[0074]

[0075]

[0076] Taking the encapsulation efficiency of rose essential oil liposome as an index, the preparation technology of rose essential oil liposome was optimized by three-factor three-level orthogonal test for the data of embodiment 1-9 in table 1, and the results are shown in table 2. It was found by using the range analysis method that the volume mass ratio of rose essential oil and lecithin was the primary factor affecting the encapsulation efficiency, followed by the mass ratio of solid phase particles and encapsulation materials. Under the experimental conditions, the volume fraction of rose essential oil in the aqueous dispersion had little effect on the encapsulation efficiency. And the volume mass ratio of rose essential oil and lecithin increased, and the encapsulation efficiency decreased, and it was more ideal to control it within θ.04. As the mass ratio of solid phase particles and encapsulation materials decreased, the encapsulation efficiency decreased, and the mass ratio was controlled at more than 3.12:1 and the effect was more ideal.

[0077] Compared with Examples 1 to 9, Comparative Example 1 did not use calamine solid phase particles as a carrier, but instead used a traditional thin film dispersion method to prepare rose essential oil liposomes. The thickness of the film formed by the encapsulating material on the wall of the rotary evaporation bottle was uneven, resulting in a low encapsulation rate of the rose essential oil liposomes.

[0078] In Comparative Examples 2 and 3, due to the rough surface of montmorillonite and the strong adsorption capacity, the encapsulated material agglomerates on the surface of montmorillonite and cannot form a spherical shape with good morphology; 2 The surface is too smooth and the support surface is difficult to wet, resulting in TiO 2 The carrier has poor adsorption capacity for the packaging material, making it difficult to form a film on its surface.

[0079] In Comparative Example 4, the liposome suspension prepared using silica gel as a solid phase particle carrier has an encapsulation efficiency lower than that of Example 1 under the same experimental conditions, indicating that the liposome suspension prepared using calamine as a solid phase particle carrier has a more ideal effect.

[0080] like Figures 1 to 3 The HPLC spectra of the free and encapsulated rose essential oil liposomes prepared in Examples 1 to 3 are shown in Figure 1. Figure 1 From the figure, it can be seen that both the free and encapsulated essential oil test solutions have peaks at the chromatographic peak of linalool, but the peak of the free essential oil test solution is smaller and close to a straight line, while the encapsulated essential oil test solution has a strong peak, indicating that the encapsulated drug leaks very little and has strong encapsulation.

[0081] like Figure 4 , 5 Shown are microscope images of the empty liposome aqueous dispersion and the rose essential oil liposome aqueous suspension in Example 1, respectively, with a magnification of 20 times. It can be seen from the figure that after the calamine particles are film-formed and hydrated on the surface, they are dispersed more evenly in water, which may be related to the Zeta potential of the particle surface after the empty liposomes and liposomes are formed.

[0082] Test Example 2: Transdermal Diffusion Experiment

[0083] Utilize the transdermal diffusion test apparatus, add the PEG400 solution with mass concentration of 40% in the diffusion cell, take 0.3mL each of the rose essential oil liposome suspension prepared in Example 3 and the rose essential oil aqueous dispersion with the same concentration and evenly disperse them on the smooth surface of the artificial skin, penetrate at room temperature, take out 0.2mL sample solution (i.e. rose essential oil liposome suspension skin penetration solution, rose essential oil aqueous dispersion skin penetration solution) from the diffusion cell after an interval of 12h for liquid chromatography determination, and make up the volume with the PEG400 solution with mass concentration of 40%, and calculate the permeability by high performance liquid chromatography. The results are shown in Table 3.

[0084] Test Example 3: Determination of Antioxidant Activity of Rose Essential Oil by DPPH Method

[0085] 1. Solution preparation

[0086] DPPH-ethanol solution: Weigh 9 mg of DPPH and dissolve it in 200 mL of ethanol. Dissolve it by ultrasonication to prepare a 0.0045 g / mL DPPH-ethanol solution.

[0087] Rose essential oil sample solution: Take rose essential oil, dissolve it in anhydrous ethanol and make up to volume to prepare a rose essential oil sample solution with a concentration of 0.06 mg / mL.

[0088] Vitamin C solution: Dissolve and dilute with anhydrous ethanol to prepare a solution with a concentration of 0.0056 mg / mL.

[0089] 2. Detection

[0090] Sample group: Take 80 μL of sample solution into a 96-well plate, add 120 μL of DDPPH free radical solution, shake well, and place at room temperature (25°C) away from light for 10 min. Measure its absorbance, which is recorded as A1;

[0091] The sample solutions are: rose essential oil sample solution, the permeation solution of Example 3 obtained in Detection Example 3, the permeation solution of the rose essential oil aqueous dispersion obtained in Detection Example 3, and the vitamin C solution.

[0092] Control group: According to the above method, the DPPH free radical solution was replaced by an equal volume of 60% (volume fraction) ethanol, and its absorbance was measured and recorded as A2;

[0093] Blank control group: Pipette 80 μL of 60% (volume fraction) ethanol into a 96-well plate, add 120 μL of DPPH free radical solution, shake well, and stand at room temperature in the dark for 10 min. Measure the absorbance, which is recorded as A0;

[0094] The scavenging rate of DPPH free radicals was calculated according to the following formula, and the results are shown in Table 3:

[0095]

[0096] Table 3 Transmission rate and scavenging rate of DPPH free radicals

[0097]

[0098] As shown in Table 3, it is the permeability of each sample and its scavenging rate to DPPH free radical, wherein ND represents not detected.As can be seen from Table 3, the skin permeation effect of the rose essential oil liposome suspension with calamine solid phase particles as carrier is weaker than that of the rose essential oil water dispersion, indicating that the liposome wrapped with rose essential oil is more closely attached to the surface of calamine particles, and is not easy to fall off and disperse in water from its surface, so it does not reflect the effect of liposome promoting rose essential oil skin penetration, and its skin permeation solution is not detected as the scavenging effect to DPPH free radical.However, as a commonly used dermatology external medicine, calamine lotion is easy to cause dry skin after use.After the liposome suspension is formed on the surface of calamine solid particles, lecithin and the rose essential oil wrapped in lecithin are retained on the skin surface, have moisturizing effect, play the effect of moisturizing skin and alleviating dry skin.Rose essential oil liposome can release rose essential oil slowly and continuously, solves the problems of rose essential oil external volatile and unstable, and has certain application value in the field of medicine and cosmetics. In addition, most essential oil fragrances exist in liquid form, and the extraction rate is low during the extraction process, and it is difficult to maintain the original fragrance of the fragrance material. Moreover, essential oil fragrances in liquid form are not conducive to the storage and transportation of fragrances, and it is not convenient to quantitatively measure during use. Rose essential oil can effectively solve the above problems by converting it into a solid form. The rose essential oil liposome suspension is dried by centrifugation, and lecithin and rose essential oil wrapped in lecithin are attached to the surface of solid particles. The solid dosage form made can be used as a solid fragrance.

Claims

1. A method for preparing rose essential oil liposomes using calamine as a carrier, characterized in that: The steps include: (a) adding a wrapping material into an anhydrous ethanol solution to prepare a wrapping material solution; (b) immersing the solid phase particles in a coating material solution, removing the anhydrous ethanol, and combining the solid phase particles with the coating material to obtain empty liposomes; (c) mixing the empty liposome with the Flos Rosae Rugosae essential oil aqueous dispersion to obtain a Flos Rosae Rugosae essential oil liposome suspension; The solid phase particles are calamine.

2. The preparation method according to claim 1, characterized in that: The packaging material is one of lecithin and lecithin-cholesterol.

3. The preparation method according to claim 2, characterized in that: In the lecithin-cholesterol, the mass ratio of lecithin to cholesterol is (4-8):1, preferably 6:

1.

4. The preparation method according to claim 1, characterized in that: The mass concentration of the encapsulating material in the encapsulating solution is 0.04726-0.2 g / mL.

5. The preparation method according to claim 1, characterized in that: The mass ratio of the calamine solid phase particles to the encapsulating material is (1.786-4.167):1, preferably (3.12-4.167):

1.

6. The preparation method according to claim 2, characterized in that: The ratio of the volume of the rose essential oil to the mass of lecithin is (0.028-0.05) mL / g, preferably (0.028-0.04) mL / g.

7. The preparation method according to claim 1, characterized in that: The volume fraction of the rose essential oil aqueous dispersion is 0.025-0.1%.

8. A rose essential oil liposome prepared by the preparation method according to any one of claims 1 to 7, characterized in that: The encapsulation rate of the rose essential oil liposome is 44.9-96.9%.

9. Use of the rose essential oil liposome prepared by the preparation method according to any one of claims 1 to 7 or the rose essential oil liposome according to claim 8 in the preparation of a skin external preparation, characterized in that: The dosage form of the rose essential oil liposome is a suspension or a semisolid, preferably an ointment.

10. Use of the rose essential oil liposome prepared by the preparation method according to any one of claims 1 to 7 or the rose essential oil liposome according to claim 8 in the preparation of solid flavors, characterized in that: The dosage form of the rose essential oil liposome is a solid dosage form, preferably a powder.

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