Micro-droplet production equipment

Abstract

The embodiment of the invention provides micro-droplet production equipment. The micro-droplet production equipment comprises a continuous phase liquid supply assembly, a dispersed phase assembly, a micro-droplet cutting assembly and a flow collecting assembly, the dispersed phase liquid supply assembly comprises at least one injection pump, and the injection pump is communicated with the dispersed phase container through a pipeline. And the continuous phase liquid supply assembly and the pipeline are communicated with the continuous phase container. The micro-droplet cutting assembly comprises at least one micro-fluidic chip; the micro-fluidic chip is communicated with the injection pump through a pipeline so as to obtain dispersed phase liquid, and is communicated with the continuous phase liquid supply assembly through a pipeline so as to obtain continuous phase liquid. The flow collecting assembly comprises a flow collecting unit and a collecting unit; the confluence unit is communicated with the micro-fluidic chip through a pipeline, and the collection unit is communicated with the confluence unit through a pipeline so as to collect the micro-droplets. According to the embodiment of the invention, through cooperation of the dispersed phase liquid supply assembly, the continuous phase liquid supply assembly, the micro-fluidic chip and the flow collecting assembly, the continuous phase liquid and the dispersed phase liquid can be continuously supplied to the micro-fluidic chip, so that the production rate of micro-droplets is increased.

Description

Technical Field

[0001] The present disclosure relates to the field of micro-droplet production technology, and in particular to a micro-droplet production device. Background Art

[0002] Microdroplets are widely used in various fields. Microfluidic technology based on microdroplets has been rapidly developed and applied in the fields of digital PCR, single cell culture, single cell genome / transcriptome sequencing, single cell function sorting, high-throughput reaction screening, protein crystallization, etc. The generation of microdroplets is to generate emulsified microdroplets using two immiscible phases. The microdroplet phase is called the dispersed phase, and the phase that encapsulates the microdroplets is called the continuous phase. However, when the existing production equipment needs to replenish raw materials, the device must be stopped intermittently, which often leads to differences between product batches and equipment losses; at the same time, since some solutions will destroy their own stability after long-term storage, they need to be reconfigured according to storage conditions and cycles, and thus the requirements of microfluidic continuous production cannot be achieved, which hinders the development of microfluidic industrialization. Therefore, in view of the above problems, it is necessary to improve the existing technology to overcome the defects in the existing technology. Summary of the invention

[0003] In view of the above-mentioned shortcomings of the prior art, the purpose of the present disclosure is to provide a micro-droplet production device to solve the problems in the related art.

[0004] The first aspect of the present disclosure provides a micro-droplet production device, comprising:

[0005] The dispersed phase liquid supply assembly comprises at least one injection pump; the injection pump pipeline is connected to the dispersed phase container;

[0006] A continuous phase liquid supply assembly, the pipeline of which is connected to the continuous phase container;

[0007] The micro-droplet cutting component comprises at least one microfluidic chip; the pipeline of the at least one microfluidic chip is connected to the injection pump to obtain the dispersed phase liquid, and the pipeline is connected to the continuous phase liquid supply component to obtain the continuous phase liquid;

[0008] The current collecting component comprises a confluence unit and a collecting unit; the confluence unit pipeline is connected to the microfluidic chip, and the collecting unit pipeline is connected to the confluence unit to collect micro droplets.

[0009] In an embodiment of the first aspect, the microfluidic chip includes a continuous phase flow channel, a dispersed phase flow channel and a micro-droplet collection chamber; the continuous phase flow channel is for loading a continuous phase liquid, and has a first inlet and a first outlet; the dispersed phase flow channel is located in the continuous phase flow channel, for loading a dispersed phase liquid, and has a second inlet and a second outlet surrounded by the first outlet; a certain angle is formed between the flow direction of the dispersed phase flow channel and the continuous phase flow channel, so that the dispersed phase liquid is cut by the continuous phase liquid of the first outlet at the second outlet to form micro-droplets; the micro-droplet collection chamber is connected to the first outlet and the second outlet.

[0010] In an embodiment of the first aspect, at least one of the continuous phase flow channel and the dispersed phase flow channel is provided with a diameter-reducing section whose inner diameter decreases along the flow direction.

[0011] In an embodiment of the first aspect, the injection pump includes a cavity to be infused and a heating component surrounding the cavity to be infused; a adapter assembly is provided between the injection pump and the microfluidic chip; the adapter assembly includes at least one adapter, a continuous phase tube and an insulation tube; the adapter has an insulation cavity, and a first interface and a second interface connected to the insulation cavity; the first interface pipeline is connected to the heating component; one end of the insulation tube is connected to the second interface, and the other end is connected to the heating medium container; the continuous phase tube is arranged in the insulation tube, and one end is connected to the microfluidic chip, and the other end extends out of the insulation cavity after passing through the second interface and is connected to the cavity to be infused.

[0012] In an embodiment of the first aspect, the injection pump includes a cavity to be infused and a heating component surrounding the cavity to be infused; a transfer assembly is provided between the injection pump and the microfluidic chip; the transfer assembly includes a first transfer joint, a second transfer joint, a continuous phase tube and an insulation tube; the first transfer joint has a first insulation cavity, and a first interface and a second interface connected to the first insulation cavity; the second transfer joint in a pair of transfer joints has a second insulation cavity, and a third interface and a fourth interface connected to the second insulation cavity; the first interface pipeline is connected to the heating component; one end of the insulation tube is connected to the second interface, and the other end is connected to the fourth interface; the third interface pipeline is connected to a heating medium container; the continuous phase tube is arranged in the insulation tube, and one end extends out of the first insulation cavity after passing through the second interface and is connected to the cavity to be infused; the other end extends out of the second insulation cavity after passing through the fourth interface and is connected to the microfluidic chip.

[0013] In an embodiment of the first aspect, a cleaning unit is further included; the cleaning unit includes a cleaning tube and at least one cleaning port connected to the cleaning tube, and the at least one cleaning port pipeline is connected to the at least one injection pump.

[0014] In an embodiment of the first aspect, a clean water unit is further included; the clean water unit includes a clean water pipe and at least one clean water port connected to the clean water pipe, and the at least one clean water port pipeline is connected to the at least one injection pump.

[0015] In an embodiment of the first aspect, the microfluidic chip is implemented as a plurality; and the plurality of microfluidic chips are all connected to the at least one injection pump by pipelines.

[0016] In an embodiment of the first aspect, the injection pump and the microfluidic chip are both implemented in plurality; each of the microfluidic chips is connected to one of the injection pumps via a pipeline.

[0017] In an embodiment of the first aspect, the number of the injection pumps is no greater than the number of the microfluidic chips.

[0018] As described above, the embodiment of the present disclosure provides a micro-droplet production device, including a continuous phase liquid supply component, a dispersed phase component, a micro-droplet cutting component and a current collecting component. The dispersed phase liquid supply component includes at least one injection pump, and the injection pump pipeline is connected to the dispersed phase container. The continuous phase liquid supply component, the pipeline is connected to the continuous phase container. The micro-droplet cutting component includes at least one microfluidic chip; the pipeline of the at least one microfluidic chip is connected to the injection pump to obtain the dispersed phase liquid, and the pipeline is connected to the continuous phase liquid supply component to obtain the continuous phase liquid. The current collecting component includes a confluence unit and a collection unit; the confluence unit pipeline is connected to the microfluidic chip, and the collection unit pipeline is connected to the confluence unit to collect micro-droplets. Through the cooperation of the dispersed phase liquid supply component, the continuous phase liquid supply component, the microfluidic chip and the current collecting component, the embodiment of the present disclosure can continuously supply continuous phase liquid and dispersed phase liquid to the microfluidic chip to increase the production rate of micro-droplets. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 , which is a schematic diagram of the structural connection of the micro-droplet production device in the embodiment of the present disclosure;

[0020] Figure 2 The figure shows the overall structure of the micro-droplet production device in the embodiment of the present disclosure;

[0021] Figure 3 , which is a cross-sectional schematic diagram of the overall structure of the adapter assembly in the embodiment of the present disclosure;

[0022] Figure 4 The embodiment of the present disclosure is shown in Figure 3 A magnified view of middle;

[0023] Figure 5 FIG. 1 is a schematic diagram of the overall structure of the adapter assembly in another embodiment of the present disclosure;

[0024] Figure 6 , which is a schematic diagram of a microfluidic chip in an embodiment of the present disclosure;

[0025] Figure 7 The embodiment of the present disclosure is shown in Figure 6 Enlarged view of middle B;

[0026] Figure 8 , which is a cross-sectional schematic diagram of the disassembly and connection of the microfluidic chip in an embodiment of the present disclosure;

[0027] Fig. 9 Schematic diagram of the overall structure of the injection pump in the embodiment of the present disclosure is shown in FIG.

[0028] Fig.10 FIG. 1 is a cross-sectional schematic diagram of the overall structure of the injection pump in an embodiment of the present disclosure;

[0029] Fig.11 FIG. 1 is a cross-sectional schematic diagram of a first embodiment of a serpentine heating channel in the present disclosure;

[0030] Fig.12 FIG. 1 is a schematic diagram of the overall structure of the first embodiment of the serpentine-shaped heating channel in the present disclosure;

[0031] Fig.13 FIG. 1 is a cross-sectional schematic diagram of a second embodiment of a serpentine heating channel in the present disclosure;

[0032] Fig.14 FIG. 1 is a schematic diagram of the overall structure of the second embodiment of the serpentine-shaped heating channel in the present disclosure;

[0033] Fig.15 FIG. 1 is a schematic diagram showing the development of the second embodiment of the serpentine heating channel in the present disclosure;

[0034] Fig.16 FIG. 1 is a cross-sectional schematic diagram of the overall structure in which the heating component of the present disclosure is implemented as a heating wire;

[0035] Fig.17 FIG. 1 is a cross-sectional schematic diagram of the overall structure of the collecting unit in the embodiment of the present disclosure;

[0036] Fig.18 FIG. 1 is a cross-sectional schematic diagram of a collection unit from another perspective in an embodiment of the present disclosure;

[0037] Fig.19 , which is a cross-sectional schematic diagram of a liquid collecting tube in an embodiment of the present disclosure;

[0038] Fig. 20 , which is a cross-sectional schematic diagram of another perspective of the liquid collecting tube in the embodiment of the present disclosure;

[0039] Fig.21 FIG. 8 is a cross-sectional schematic diagram of another embodiment of the medium arrangement tube in the disclosed embodiment.

[0040] Reference numerals:

[0041] 100, dispersed phase liquid supply assembly; 110, injection pump; 111, injection pump body; 1111, infusion chamber; 11111, injection port; 11112, infusion port; 1112, piston block; 1113, sealing plug; 1114, insulation layer; 112, heating component; 1121, heating flow channel; 11211, second liquid outlet; 11212, second liquid inlet; 1122, heating wire; 120, dispersed phase container; 130, cleaning unit; 131, cleaning pipe; 1301, cleaning port; 140, clean water unit; 141, clean water pipe; 1401, clean water port;

[0042] 200, continuous phase liquid supply assembly; 210, continuous phase container; 220, continuous phase liquid supply pipe;

[0043] 300, microfluidic chip; 310, first substrate; 320, second substrate; 321, second base block; 322, liquid guide tube; 323, sealing member; 301, continuous phase flow channel; 3011, first inlet; 3012, first outlet; 3013, second diameter reducing section; 302, dispersed phase flow channel; 3021, second inlet; 3022, second outlet; 3023, first diameter reducing section; 303, micro droplet collection chamber; 3031, guide portion; 3032, third liquid outlet;

[0044] 400, current collecting assembly; 410, confluence unit; 420, collecting unit; 421, collecting container; 4211, collecting chamber; 42111, first channel; 42112, second channel; 4212, top opening; 4213, collecting cylinder; 4214, cover plate; 4215, bottom opening; 4216, guide portion; 422, partition plate; 433, medium discharge pipe; 4331, liquid inlet; 4332, liquid discharge port; 4333, vertical pipe section; 4334, inclined pipe section; 434, liquid collecting pipe.

[0045] 500, adapter assembly; 510, adapter; 5101, insulation chamber; 5102, first interface; 5103, second interface; 520, continuous phase tube; 530, insulation tube; 500A, adapter assembly; 510A, first adapter; 5101A, first insulation chamber; 5102A, third interface; 5103A, fourth interface; 510B, second adapter; 5101B, second insulation chamber; 5102B, fifth interface; 5103B, sixth interface. DETAILED DESCRIPTION

[0046] The following is an explanation of the embodiments of the present disclosure by specific examples, and those skilled in the art can easily understand other advantages and effects of the present disclosure from the information disclosed by the present disclosure. The present disclosure can also be implemented or applied through other different specific embodiments, and the details in the present disclosure can also be modified or changed in various ways according to different viewpoints and application modules without departing from the spirit of the present disclosure. It should be noted that the embodiments in the present disclosure and the features in the embodiments can be combined with each other without conflict.

[0047] The following is a detailed description of the embodiments of the present disclosure with reference to the accompanying drawings so that those skilled in the art can easily implement the present disclosure. The present disclosure can be embodied in many different forms and is not limited to the embodiments described herein.

[0048] In the representations of the present disclosure, the reference terms "one embodiment", "some embodiments", "examples", "specific examples", or "some examples" and the like mean that the specific features, structures, materials or characteristics represented in conjunction with the embodiment or example are included in at least one embodiment or example of the present disclosure. Moreover, the specific features, structures, materials or characteristics represented may be combined in any one or a group of embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine different embodiments or examples represented in the present disclosure and the features of different embodiments or examples, unless they are mutually contradictory.

[0049] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the representation of the present disclosure, "a group" means two or more, unless otherwise clearly and specifically defined.

[0050] In order to clearly describe the present disclosure, components not related to the description are omitted, and the same reference numerals are given to the same or similar components throughout the specification.

[0051] Throughout the specification, when a device is said to be "connected" to another device, this includes not only the case of "direct connection" but also the case of "indirect connection" by placing other elements therebetween. In addition, when a device is said to "include" a certain component, unless otherwise stated, it does not exclude other components, but means that other components may be included.

[0052] Although the terms first, second, etc. are used to represent various elements in this article in some examples, these elements should not be limited by these terms. These terms are only used to distinguish one element from another element. For example, the first interface and the second interface, etc. are represented. Moreover, as used in this article, the singular forms "one", "one" and "the" are intended to also include plural forms, unless there is an opposite indication in the context. It should be further understood that the terms "comprising" and "including" indicate that there are the described features, steps, operations, elements, modules, projects, kinds, and / or groups, but do not exclude the existence, occurrence or addition of one or a group of other features, steps, operations, elements, modules, projects, kinds, and / or groups. The terms "or" and "and / or" used herein are interpreted as inclusive, or mean any one or any combination. Therefore, "A, B or C" or "A, B and / or C" means "any of the following: A; B; C; A and B; A and C; B and C; A, B and C". Only when the combination of elements, functions, steps or operations is inherently mutually exclusive in some way, will there be an exception to this definition.

[0053] The technical terms used herein are only used to refer to specific embodiments and are not intended to limit the present disclosure. The singular form used herein also includes the plural form unless the sentence clearly indicates the contrary meaning. The meaning of "including" used in the specification is to specify specific characteristics, regions, integers, steps, operations, elements and / or components, and does not exclude the existence or addition of other characteristics, regions, integers, steps, operations, elements and / or components.

[0054] Although not defined differently, all terms, including technical and scientific terms used herein, have the same meaning as those generally understood by those skilled in the art to which the present disclosure belongs. Terms defined in commonly used dictionaries are additionally interpreted as having meanings consistent with relevant technical literature and the message of the present disclosure, and shall not be overly interpreted as ideal or very formal meanings unless defined.

[0055] Microdroplets are widely used in various fields. Microfluidic technology based on microdroplets has been rapidly developed and applied in the fields of digital PCR, single cell culture, single cell genome / transcriptome sequencing, single cell function sorting, high-throughput reaction screening, protein crystallization, etc. The generation of microdroplets is to generate emulsified microdroplets using two immiscible phases. The microdroplet phase is called the dispersed phase, and the phase that encapsulates the microdroplets is called the continuous phase. However, when the existing production equipment needs to replenish raw materials, the device must be stopped intermittently, which often leads to differences between product batches and equipment losses; at the same time, since some solutions will destroy their own stability after long-term storage, they need to be reconfigured according to storage conditions and cycles, and thus the requirements of microfluidic continuous production cannot be achieved, which hinders the development of microfluidic industrialization. Therefore, in view of the above problems, it is necessary to improve the existing technology to overcome the defects in the existing technology.

[0056] Based on the above problems, the embodiment of the present disclosure can continuously supply continuous phase liquid and dispersed phase liquid to the microfluidic chip through the cooperation of the dispersed phase liquid supply component, the continuous phase liquid supply component, the microfluidic chip and the collecting component to improve the production rate of microdroplets.

[0057] Figure 1 Shown in the figure is a schematic diagram of the structural connection of the micro-droplet production equipment in the embodiment of the present disclosure. Figure 2 The figure shows the overall structure of the micro-droplet production device in the embodiment of the present disclosure. Figure 1 and Figure 2 In the example, the micro-droplet production device includes a dispersed phase liquid supply component 100, a continuous phase liquid supply component 200, a micro-droplet cutting component and a current collecting component 400. The dispersed phase liquid supply component 100 includes at least one injection pump 110, and the pipeline of the injection pump 110 is connected to the dispersed phase container 120. The pipeline of the continuous phase liquid supply component 200 is connected to the continuous phase container 210. The micro-droplet cutting component includes at least one microfluidic chip 300; the pipeline of the at least one microfluidic chip 300 is connected to the injection pump 110 to obtain the dispersed phase liquid, and the pipeline is connected to the continuous phase liquid supply component 200 to obtain the continuous phase liquid. The current collecting component 400 includes a confluence unit 410 and a collection unit 420; the pipeline of the confluence unit 410 is connected to the microfluidic chip 300, and the pipeline of the collection unit 420 is connected to the confluence unit 410 to collect micro-droplets.

[0058] The advantage of the above arrangement is that, through the cooperation of the dispersed phase liquid supply component 100, the continuous phase liquid supply component 200, the microfluidic chip 300 and the current collecting component 400, the continuous phase liquid and the dispersed phase liquid can be continuously supplied to the microfluidic chip 300 to increase the production rate of microdroplets.

[0059] Exemplarily, the injection pump 110 and the microfluidic chip 300 are both implemented in multiples; each of the microfluidic chips 300 is connected to one of the injection pumps 110 through a pipeline. Preferably, the number of the injection pumps 110 is not greater than the number of the microfluidic chips 300. For example, the number of the microfluidic chips 300 is an integer multiple of the injection pumps 110. That is, one injection pump 110 simultaneously supplies dispersed phase liquid to multiple microfluidic chips 300 through a pipeline.

[0060] In another embodiment, only the microfluidic chip 300 is implemented as a plurality of microfluidic chips 300; the plurality of microfluidic chips 300 are all connected to the at least one injection pump 110 through a pipeline. For example, the at least one injection pump 110 has a plurality of infusion ports, and each microfluidic chip 300 is connected to one of the infusion ports through a pipeline.

[0061] Figure 3 The figure shows a cross-sectional view of the overall structure of the adapter assembly in the embodiment of the present disclosure. Figure 2 and Figure 3 In the example, the micro-droplet production device further includes a cleaning unit 130; the cleaning unit 130 includes a cleaning pipe 131 and at least one cleaning port 1301 connected to the cleaning pipe 131, and the at least one cleaning port 1301 is connected to the at least one injection pump 110. In another exemplary embodiment, the micro-droplet production device further includes a clean water unit 140; the clean water unit 140 includes a clean water pipe 141 and at least one clean water port 1401 connected to the clean water pipe 141, and the at least one clean water port 1401 is connected to the at least one injection pump 110. The advantage of the above arrangement is that the cleaning liquid is injected into the injection pump 110 through the cleaning unit 130 to clean the injection pump 110, and then the cleaning liquid remaining in the injection pump 110 is rinsed clean by the clean water unit 140. Thereby, the qualified rate of micro-droplets is avoided to be reduced due to the failure to clean the injection pump 110 in time.

[0062] Exemplarily, the cleaning port 1301 and the clean water port 1401 are configured to be connected to the same opening of the injection pump 110 ; they may also be configured to be connected to different openings of the injection pump 110 .

[0063] Exemplarily, the confluence unit 410 is implemented as a plurality of confluence tubes (not shown in the figure), and the number of the plurality of confluence tubes matches the number of the microfluidic chips 300; the first port of each confluence tube is connected to one of the microfluidic chips 300 through a pipeline, and the second ports of the plurality of confluence tubes can be directly connected to the collection unit 420 through a separate pipeline, or can be connected to the collection unit 420 through a pipeline after being connected in parallel. In another embodiment, the confluence unit 410 is implemented as a joint to connect the microfluidic chip 300 and the collection unit 420.

[0064] exist Figure 3 In the example, the continuous phase liquid supply component 200 is implemented to include at least one continuous phase liquid supply tube 220, one end of the at least one continuous phase liquid supply tube 220 is connected to the continuous phase container 210, and the other end is connected to the microfluidic chip 300. Further exemplarily, when the microfluidic chip 300 is implemented as a plurality, the continuous phase liquid supply tube 220 is implemented to match the number of the microfluidic chips 300.

[0065] Figure 4 The embodiment of the present disclosure is shown in Figure 3 A magnified view of the middle. Figure 3 and Figure 4 In the example, the injection pump 110 includes a cavity 1111 to be infused and a heating component 112 surrounding the cavity 1111 to be infused, and the heating component 112 is implemented as a heating corridor 1121. An adapter assembly 500 is provided between the injection pump 110 and the microfluidic chip 300; the adapter assembly 500 includes at least one adapter 510, a dispersed phase tube 520 and a heat preservation tube 530. Exemplarily, the adapter 510 is detachably provided on the injection pump 110, such as by snap-fitting or screwing. The adapter 510 has a heat preservation chamber 5101, and a first interface 5102 and a second interface 5103 connected to the heat preservation chamber 5101; the first interface 5102 pipeline is connected to the heating component 112; one end of the heat preservation tube 530 is connected to the second interface 5103, and the other end is connected to the heating medium container (not shown in the figure); the dispersed phase tube 520 is arranged in the heat preservation tube 530, and one end is connected to the microfluidic chip 300, and the other end extends out of the heat preservation chamber 5101 after passing through the second interface 5103 and is connected to the infusion chamber 1111. The advantage of the above arrangement is that the dispersed phase liquid flowing out of the injection pump 110 can be kept warm, and the dispersed phase liquid can be prevented from solidifying due to heat loss during the transportation process.

[0066] Figure 5 The figure shows the overall structure diagram of the adapter assembly in another embodiment of the present disclosure. Figure 5In the example, the injection pump 110 includes a cavity 1111 to be infused and a heating component 112 surrounding the cavity 1111 to be infused; an adapter assembly 500A is provided between the injection pump 110 and the microfluidic chip 300; the adapter assembly 500 includes a first adapter 510A, a second adapter 510B, a dispersed phase tube 520 and a heat preservation tube 530; the first adapter 510A has a first heat preservation cavity 5101A, and a third interface 5102A and a fourth interface 5103A connected to the first heat preservation cavity 5101A; the second adapter 510B in a pair of adapters has a second heat preservation cavity 5101B, and a third interface 5102A and a fourth interface 5103A connected to the second heat preservation cavity 5101B The fifth interface 5104B and the sixth interface 5105B of the embodiment; the third interface 5102A pipeline is connected to the heating component 112; one end of the insulation tube 530 is connected to the fourth interface 5103A, and the other end is connected to the sixth interface 5105B; the fifth interface 5104B pipeline is connected to the heating medium container (not shown in the figure); the dispersed phase tube 520 is arranged in the insulation tube 530, and one end extends out of the first insulation cavity 5101A after passing through the fourth interface 5103A and is connected to the infusion cavity 1111; the other end extends out of the second insulation cavity 5101B after passing through the sixth interface 5105B and is connected to the microfluidic chip 300. This embodiment can further prevent the dispersed phase liquid from solidifying due to heat loss during the transportation process.

[0067] Figure 6 Shown in FIG. 1 is a schematic diagram of a microfluidic chip in an embodiment of the present disclosure. Figure 7 The embodiment of the present disclosure is shown in Figure 6 A magnified view of B. Figure 6 and Figure 7 In the example, the microfluidic chip 300 includes a continuous phase flow channel 301, a dispersed phase flow channel 302 and a micro-droplet collection chamber 303. The continuous phase flow channel 301 is used to load the continuous phase liquid, and has a first inlet 3011 and a first outlet 3012. The dispersed phase flow channel 302 is located in the continuous phase flow channel 301, and is used to load the dispersed phase liquid, and has a second inlet 3021 and a second outlet 3022 surrounded by the first outlet 3012; a certain angle is formed between the dispersed phase flow channel 302 and the flow direction of the continuous phase flow channel 301, so that the dispersed phase liquid is cut by the continuous phase liquid of the first outlet 3012 at the second outlet 3022 to form micro-droplets. The micro-droplet collection chamber 303 is connected to the first outlet 3012 and the second outlet 3022. It can be understood that the continuous phase liquid supply pipe is connected to the continuous phase flow channel 301, and the dispersed phase liquid supply pipe is connected to the dispersed phase flow channel 302.

[0068] The advantage of the above arrangement is that the annular continuous phase flow channel 301 can form a micro-droplet collection chamber 303 surrounding the second outlet 3022, and the continuous phase liquid in the micro-droplet collection chamber 303 can wrap a large number of dispersed phase droplets flowing out of the second outlet 3022, thereby solving the problem of slow micro-droplet production rate caused by insufficient supply of the continuous phase liquid and increasing the yield of micro-droplets.

[0069] As a type of microdroplets, microspheres have a wide range of applications in many fields, including drug delivery, chemical separation, catalytic reactions and biomedical applications.

[0070] exist Figure 6 and Figure 7 In the example, at least the area in the dispersed phase flow channel 302 surrounded by the continuous phase flow channel 301 is implemented as a first diameter-reducing section 3023, and the inner diameter of the first diameter-reducing section 3023 decreases along the flow direction. It can be understood that when the dispersed phase liquid flows through the first diameter-reducing section 3023, as the inner diameter gradually decreases along the flow direction, the flow velocity of the dispersed phase liquid in the dispersed phase flow channel 302 is increased, thereby increasing the velocity at which the dispersed phase liquid flows out of the second outlet 3022 and forms dispersed phase droplets, thereby increasing the yield of micro-droplets.

[0071] exist Figure 6 and Figure 7 In the example, the continuous phase flow channel 301 at least surrounds the dispersed phase flow channel 302 and is implemented as a second diameter-reducing section 3013, and the inner diameter of the second diameter-reducing section 3013 decreases along the flow direction. It can be understood that when the continuous phase liquid flows through the second diameter-reducing section 3013, as the inner diameter gradually decreases along the flow direction, the flow rate of the continuous phase liquid in the continuous phase flow channel 301 is increased, thereby increasing the speed at which the continuous phase liquid flows into the micro-droplet collection chamber 303 and accumulates therein in large quantities, thereby being able to wrap a large number of dispersed phase droplets flowing out of the second outlet 3022, avoiding the problem of micro-droplets being unable to form due to insufficient supply of the continuous phase liquid, and further increasing the yield of micro-droplets.

[0072] Exemplarily, the first reducing section 3023 and the second reducing section 3013 can be implemented alone or both at the same time. In this embodiment, the first reducing section 3023 and the second reducing section 3013 are implemented at the same time. It can be understood by those skilled in the art that the larger the angle between the second reducing section 3013 and the liquid flow direction, the greater the cutting force applied by the continuous phase liquid flowing out of the first outlet 3012 to the dispersed phase liquid flowing out of the second outlet 3022 in the direction perpendicular to the liquid flow direction, that is, the better the cutting effect on the dispersed phase liquid flowing out of the second outlet 3022, thereby greatly improving the production rate of micro-droplets.

[0073] Exemplarily, the inner diameter of the micro-droplet collecting chamber 303 is larger than the inner diameter of the second outlet 3022. It is understandable that the micro-droplet collecting chamber 303 with a larger inner diameter can accommodate a large amount of continuous phase liquid at the second outlet 3022, thereby meeting the need to wrap a large amount of dispersed phase droplets flowing out of the second outlet 3022, thereby avoiding the problem of micro-droplets being unable to form due to insufficient supply of the continuous phase liquid.

[0074] Exemplarily, the first outlet 3012, the second outlet 3022 and the micro-droplet collecting chamber 303 are coaxial. Preferably, the cross section of the micro-droplet collecting chamber 303 is implemented as a circle. In other words, the second outlet 3022 is aligned with the first outlet 3012 and the micro-droplet collecting chamber 303. The advantage of such a setting is that the speed at which the finished micro-droplets formed in the micro-droplet collecting chamber 303 flow out of the first outlet 3012 can be accelerated, and the finished micro-droplets are prevented from being accumulated in the micro-droplet collecting chamber 303, thereby reducing the production speed of the micro-droplets. In another embodiment, the cross section of the micro-droplet collecting chamber 303 can also be implemented as a rectangle.

[0075] Exemplarily, the first inlet 3011 may also be implemented as two or more. When the first inlet 3011 is implemented as two, the two first inlets 3011 are symmetrically arranged. When the first inlet 3011 is implemented as multiple, the multiple first inlets 3011 are spaced and evenly distributed along the circumferential direction, so that the pressure of the continuous phase liquid in the continuous phase flow channel 301 is more uniform.

[0076] exist Figure 7 In the example, the micro-droplet collection chamber 303 includes a third liquid outlet 3032 for the discharge of micro-droplets. The aperture of the third liquid outlet 3032 is smaller than the inner diameter of the micro-droplet collection chamber 303. It can be understood that the third liquid outlet 3032 with a small aperture can reduce the amount of continuous phase liquid flowing out through the third liquid outlet 3032 to avoid waste of continuous phase liquid, and can also allow the finished micro-droplets to flow out.

[0077] Exemplarily, a conical guide portion 3031 is provided at the connection between the micro-droplet collection chamber 303 and the third liquid outlet 3032. The advantage of such a configuration is that the micro-droplets in the micro-droplet collection chamber 303 can be guided to flow toward the third liquid outlet 3032, further preventing the micro-droplets from accumulating in the micro-droplet collection chamber 303 and blocking the third liquid outlet 3032.

[0078] Further illustratively, the distance between the second outlet 3022 and the third liquid outlet 3032 is adjustable, and the diameter of the micro-droplet increases as the distance between the second outlet 3022 and the third liquid outlet 3032 increases. Those skilled in the art can understand that, firstly, the larger the distance between the second outlet 3022 and the third liquid outlet 3032, the smaller the component force (cutting force) perpendicular to the flow direction on the dispersed phase liquid flowing out of the second outlet 3022 when the continuous phase liquid flows in the micro-droplet collecting chamber 303, resulting in that the dispersed phase liquid flowing out of the second outlet 3022 cannot be quickly cut into droplets; secondly, the larger the distance between the second outlet 3022 and the third liquid outlet 3032, the longer the path for the dispersed phase liquid flowing out of the second outlet 3022 to combine with the external continuous phase liquid, ultimately resulting in a larger volume of the dispersed phase droplets flowing out of the second outlet 3022 and being cut, and the diameter of the micro-droplets of the final product increases accordingly, and conversely, the diameter of the micro-droplets of the final product decreases accordingly, so it is not elaborated here.

[0079] Figure 8 The figure shows a cross-sectional view of the disassembly and connection of the microfluidic chip in the embodiment of the present disclosure. Figure 8 In the example, the continuous phase flow channel 301 is formed in the first substrate 310; the dispersed phase flow channel 302 is formed in the second substrate 320; the first substrate 310 and the second substrate 320 are detachably connected, and after the first substrate 310 is connected to the second substrate 320, the continuous phase flow channel 301 and the dispersed phase flow channel 302 are connected.

[0080] Exemplarily, the second substrate 320 includes a detachably connected second base block 321 and a liquid conduit 322, and the dispersed phase flow channel 302 is formed on the second base block 321 and the liquid conduit 322; the wall surface of the first substrate 310 that is in contact with the second substrate 320 is provided with an insertion cavity (attached Figure 6 when the first base body 310 is connected to the second base body 320, the liquid conduit 322 is inserted into the insertion cavity to form the continuous phase flow channel 301 surrounding the dispersed phase flow channel 302. In this embodiment, the second base block 321 and the liquid conduit 322 are threadedly connected. In another embodiment, the disassembly connection method of the second base block 321 and the liquid conduit 322 is implemented as first snap connection (positioning) and then bolt connection (tightening).

[0081] Further exemplarily, a second sub-channel including a second sub-inlet and a second sub-outlet is provided on the second base block 321, and the first end of the liquid guide tube 322 is threadedly connected to the second sub-outlet. In other words, the dispersed phase channel 302 is composed of the second sub-channel and the inner cavity of the liquid guide tube 322, and the second outlet 3022 is formed at the second end of the liquid guide tube 322.

[0082] exist Figure 8 In an embodiment, the distance between the second outlet 3022 and the third liquid outlet 3032 can be adjusted by adjusting the length of the liquid guide tube 322 screwed into the second sub-outlet.

[0083] It will be appreciated by those skilled in the art that the detachable connection between the first substrate 310, the second substrate 320 and the liquid guide tube 322 can facilitate the replacement of the liquid guide tube 322 with different inner diameters to produce micro-droplets with different diameters; it can also facilitate regular cleaning of the continuous phase flow channel 301, the dispersed phase flow channel 302 and the inside of the liquid guide tube 322 to maintain the cleanliness of the inside of the chip.

[0084] Exemplarily, the first base 310 and the second base 320 are connected by bolts. For example, a first threaded hole is provided on the first base 310, and a second threaded hole is provided on the second base 320. In another embodiment, the detachable connection mode of the first base 310 and the second base 320 is implemented as a clamping connection.

[0085] exist Figure 8 In the example, a seal 323 is provided between the walls of the first substrate 310 and the second substrate 320, so as to form a seal for the continuous phase flow channel 301 and the dispersed phase flow channel 302 after the first substrate 310 and the second substrate 320 are connected. Exemplarily, the wall of the second substrate 320 that is in contact with the first substrate 310 is provided with an annular sealing groove surrounding the liquid guide tube 322, and the annular sealing groove is filled with a seal 323 implemented as a sealing ring. Further exemplarily, the insertion cavity is located in the annular sealing groove. It can be understood that the seal 323 can improve the sealing effect of the continuous phase flow channel 301 after the first substrate 310 and the second substrate 320 are connected, and prevent the liquid in the continuous phase flow channel 301 and the dispersed phase flow channel 302 from flowing out of the gap between the first substrate 310 and the second substrate 320.

[0086] Fig. 9 Shown in is a schematic diagram of the overall structure of the injection pump in an embodiment of the present disclosure. Fig.10 The figure shows a cross-sectional schematic diagram of the overall structure of the injection pump in the embodiment of the present disclosure. Fig. 9 and Fig.10In the example, the injection pump 110 includes an injection pump body 111 and a heating component 112. The injection pump body 111 includes a to-be-infused cavity 1111 for loading a continuous phase liquid. The heating component 112 is arranged to extend along the to-be-infused cavity 1111 and is arranged on the injection pump body 111 to form a covering around the to-be-infused cavity 1111.

[0087] The advantage of the above arrangement is that the heating component 112 can not only realize the basic function of heating the dispersed phase liquid in the infusion cavity 1111, but also improve the heating effect of the dispersed phase liquid in the infusion cavity 1111 through the heating medium flowing from bottom to top in the heating component 112.

[0088] Exemplarily, the heating component 112 is implemented as being disposed on the outer wall or inside the wall of the injection pump body 111 .

[0089] exist Fig.10 In the example, the heating component 112 is implemented as being disposed on the outer wall of the injection pump body 111. The heating component 112 forms a heating channel 1121 for loading a heating medium; the heating channel 1121 has a second liquid outlet 11211 disposed at the top and a second liquid inlet 11212 disposed at the bottom. Exemplarily, the heating medium is implemented as oil or water.

[0090] exist Fig.10 In the example, the injection pump body 111 further includes a liquid injection port 11111 and a liquid infusion port 11112 for communicating with the liquid infusion cavity 1111; the liquid injection port 11111 and the second liquid outlet 11211 are formed on the same wall surface of the injection pump body 111. For example, in this embodiment, the liquid injection port 11111 and the liquid infusion port 11112 are arranged on the top wall of the injection pump body 111. In another embodiment, the liquid injection port 11111 and the liquid infusion port 11112 are arranged on the bottom wall of the injection pump body 111.

[0091] In the present embodiment, the injection pump 110 further includes a heating element (not shown in the figure), which is arranged around the injection pump body 111 corresponding to the area on the heating channel 1121 close to the second liquid outlet 11211. Exemplarily, the heating element is implemented as a heating plate. It can be understood by those skilled in the art that when the heating medium flows in the heating channel 1121, the temperature gradually decreases (that is, the closer to the second liquid outlet 11211, the lower the temperature of the heating medium). Therefore, the heating element arranged in the area close to the second liquid outlet 11211 can assist in heating the dispersed phase liquid corresponding to the area of ​​the second liquid outlet 11211, which can not only increase the temperature of the dispersed phase liquid at the infusion port 11112, but also improve the temperature consistency of the dispersed phase liquid in the infusion cavity 1111.

[0092] In another embodiment, the injection port 11111 and the infusion port 11112 may also be implemented as being formed on the bottom wall of the injection pump body 111 .

[0093] Exemplarily, the injection pump body 111 further includes a piston block 1113 which can be disposed in the cavity 1111 to be infused close to or away from the infusion port 11112. It is understandable that when the piston block 1113 is close to the infusion port 11112, the dispersed phase liquid is squeezed out of the cavity 1111 to be infused through the infusion port 11112; after the piston block 1113 is away from the infusion port 11112, the dispersed phase liquid is added to the cavity 1111 to be infused through the injection port 11111.

[0094] Exemplarily, the injection pump body 111 further includes a sealing plug 1112 detachably connected to the main liquid port 1011. For example, the injection port 11111 is provided with an internal thread, and the cylindrical outer surface of the sealing plug 1112 is provided with an external thread combined with the internal thread. Further exemplarily, a sealing ring (not shown in the figure) is provided between the sealing plug 1112 and the injection pump body 111.

[0095] Exemplarily, the heating channel 1121 is disposed in a serpentine manner around the injection pump body 111 .

[0096] Fig.11 Shown in the figure is a cross-sectional schematic diagram of the first embodiment of the serpentine-shaped heating channel in the present disclosure. Fig.12 The figure shows the overall structure of the first embodiment of the serpentine heating channel in the present disclosure. Fig.11 and Fig.12 In the example, the heating component 112 implemented as the heating channel 1121 is implemented as being arranged in the wall of the injection pump body 111. The heating channel 1121 is configured to spirally surround the injection pump body 111 along the axial direction of the injection pump body 111. Exemplarily, the direction of rotation of the spiral winding can be implemented as clockwise or counterclockwise. Further exemplarily, the pitch of the spiral heating channel is configured to enable the spacing between the upper and lower adjacent heating channels 1121 to be small enough to increase the coverage area of ​​the heating channel 1121 to the cavity 1111 to be infused. The advantage of the above arrangement is that while ensuring the coverage area of ​​the heating channel 1121 to the cavity 1111 to be infused, the flow time of the heating medium in the heating channel 1121 is extended to further improve the heating effect of the heating medium on the dispersed phase liquid in the cavity 1111 to be infused.

[0097] Fig.13 Shown in the figure is a cross-sectional schematic diagram of the second embodiment of the serpentine heating channel in the present disclosure. Fig.14 Shown in the figure is a schematic diagram of the overall structure of the second embodiment of the serpentine heating channel in the present disclosure. Fig.15 The figure shows a schematic diagram of the second embodiment of the serpentine heating channel in the present disclosure. Fig.13 , 14 and Fig.15 In the example, the heating component implemented as the heating channel 1121 is implemented as being disposed in the wall of the injection pump body 111. The heating channel 1121 is configured to snake around the injection pump body 111 along the circumference of the injection pump body 111. It can be understood by those skilled in the art that, in this embodiment, the heating medium first flows upward along the circumference of the injection pump body 111 to the top of the injection pump body 111, then flows downward to the bottom of the injection pump body 111, and then repeats the above process.

[0098] In this way, the heating medium can flow back and forth between the top and bottom of the injection pump body 111, and transfer heat alternately to the top and bottom of the injection pump body 111, thereby avoiding the situation where the heat transfer of the heating medium can only be from the bottom to the top and the heat attenuation at the top occurs, thereby improving the uniform heating of the dispersed phase liquid in the infusion cavity 1111 by the heating medium. Secondly, the heating effect of the heating medium on the dispersed phase liquid in the infusion cavity 1111 can be further improved by extending the flow time of the heating medium in the heating channel 1121.

[0099] Fig.16 The figure shows a cross-sectional view of the overall structure of the heating component in the present disclosure being implemented as a heating wire. Fig.16 In the example, the heating component 112 is implemented as a heating wire 1122, and the heating wire 1122 is serpentinely wrapped around the injection pump body 111 along the axial direction of the injection pump body 111. For example, the winding method of the heating wire 1122 is similar to Fig.12 The winding method in the example is the same.

[0100] In the above embodiment, the injection pump 110 further includes a heat-insulating layer 1114. The heat-insulating layer 1114 is coated on the annular side wall of the injection pump body 111. Exemplarily, the heat-insulating layer 1114 is implemented as heat-insulating cotton or an inert gas layer. The advantage of such a setting is that it can reduce the heat loss of the dispersed phase liquid in the infusion cavity 1111, and can also reduce the heat transfer between the heating medium and the outside world.

[0101] Fig.17 Shown in is a cross-sectional schematic diagram of the overall structure of the collection unit in an embodiment of the present disclosure. Fig.18 The figure shows a cross-sectional schematic diagram of the collection unit from another perspective in the embodiment of the present disclosure. Fig.17 and Fig.18 In the example, the collection unit 420 includes a collection container 421, a partition 422 and a medium discharge pipe 433. The collection container 421 includes a collection chamber 4211 with a top opening 4212. The partition 422 is vertically arranged in the collection chamber 4211 to separate a portion of the space of the collection chamber 4211 from the top opening 4212 into a first channel 42111 and a second channel 42112 separated from each other; wherein the first channel 42111 is connected to the top opening 4212; and the top opening 4212 is for the medium containing micro-droplets to enter. The medium discharge pipe 433 includes a liquid inlet 4331 arranged in the second channel 42112 and extending to the outside of the collection chamber 4211.

[0102] The advantage of the above arrangement is that the partition 422 is used to separate the first channel 42111 through which the micro-droplets enter the collection chamber 4211 and the liquid inlet 4331 of the medium discharge pipe 433, thereby preventing the micro-droplets entering the collection chamber 4211 from entering the liquid inlet 4331 and being mistakenly discharged from the collection chamber 4211, thereby indirectly increasing the yield of micro-droplets by avoiding the waste of micro-droplets.

[0103] Exemplarily, the distance between the liquid inlet 4331 and the top opening 4212 is smaller than the distance between the bottom wall of the partition 422 and the top opening 4212. The advantage of such a configuration is that it can prevent the micro-droplets entering the first channel 42111 from falling into the liquid inlet 4331 of the medium discharge pipe 433 after passing over the partition 422 downward, and from being mistakenly discharged outside the collection chamber 4211, thereby causing waste of micro-droplets. Exemplarily, one end of the medium discharge pipe 433 extending outside the collection chamber 4211 is configured as a liquid discharge port 4332.

[0104] Exemplarily, the collection chamber 4211 also includes a bottom outlet 4215 for the discharge of micro-droplets. It can be understood by those skilled in the art that the method of use of the embodiment of the present disclosure is as follows: the liquid entering the collection chamber 4211 is a micro-droplet and a continuous phase liquid with a density difference, so after entering the collection chamber 4211, the micro-droplet sinks to the bottom of the collection chamber 4211, while the continuous phase liquid is located on the upper layer of the micro-droplet. As the collection chamber 4211 is gradually filled with micro-droplets and continuous phase liquid, the liquid level of the continuous phase liquid is guided to the collection chamber 4211 by the medium discharge pipe 433 when it submerges the liquid inlet 4331, that is, it is separated from the micro-droplet, and the micro-droplet is discharged from the collection chamber 4211 through the bottom outlet 4215 after the micro-droplet in the collection chamber 4211 reaches a certain capacity.

[0105] Further illustratively, the area of ​​the collecting container 421 surrounding the bottom outlet 4215 is configured as a conical guide portion 4216; the inner diameter of the guide portion 4216 increases along the direction in which the bottom outlet 4215 moves toward the top opening 4212. It can be understood by those skilled in the art that the conical guide portion 4216 can both speed up the discharge of the micro-droplets in the collecting chamber 4211 from the bottom outlet 4215 and prevent the micro-droplets from remaining on the bottom wall of the collecting chamber 4211, thereby further avoiding the waste of micro-droplets and indirectly increasing the yield of micro-droplets.

[0106] Fig.19 FIG. 4 is a cross-sectional schematic diagram of a liquid collecting tube included in an embodiment of the present disclosure. Fig. 20 FIG. 1 is a cross-sectional schematic diagram of another perspective of the liquid collecting tube in the embodiment of the present disclosure. Fig.19 and Fig. 20 In the example, the collecting unit 420 further includes a liquid collecting pipe 434 disposed in the top opening 4212. The partition 422 is vertically disposed in the liquid collecting pipe 434, the first channel 42111 and the second channel 42112 are formed in the inner cavity of the liquid collecting pipe 434; the collecting cavity 4211 further includes a bottom outlet 4215 for discharge of micro-droplets; the liquid collecting pipe 434 and the bottom outlet 4215 are coaxial.

[0107] Exemplarily, the distance between the liquid inlet 4331 and the top opening 4212 is smaller than the distance between the bottom wall of the partition 422 and the top opening 4212 and the distance between the bottom wall of the liquid collecting pipe 434 and the top opening 4212 .

[0108] The advantage of the above arrangement is that the liquid collecting pipe 434 can guide the micro-droplets to fall into the area corresponding to the bottom outlet 4215, so as to discharge the micro-droplets from the bottom outlet 4215 later. It can also reduce the range in which the liquid inlet 4331 can communicate with the liquid surface, thereby further reducing the possibility of micro-droplets being discharged from the liquid inlet 4331, thereby avoiding the waste of micro-droplets and indirectly increasing the yield of micro-droplets.

[0109] exist Fig.19In the example, the collection container 421 includes a detachably connected collection barrel 4213 and a cover plate 4214; the space enclosed by the collection barrel 4213 and the cover plate 4214 forms the collection chamber 4211; the top opening 4212 is formed on the cover plate 4214, and the bottom outlet 4215 is formed on the bottom wall of the collection barrel 4213. Exemplarily, the disassembly connection mode of the collection barrel 4213 and the cover plate 4214 is implemented as a screw connection or a snap-on connection. It can be understood that the disassembly connection of the collection barrel 4213 and the cover plate 4214 can facilitate the operator to clean the inside of the collection chamber 4211 and the medium discharge pipe 433. In this embodiment, the collection barrel 4213 and the cover plate 4214 are connected by bolts. In another embodiment, the collection barrel 4213 and the cover plate 4214 are snap-on connected.

[0110] exist Fig.18 and Fig.19 In the example, the medium discharge pipe 433 has a plurality of bends. In other embodiments, the medium discharge pipe 433 has a bend, for example Fig.21 A cross-sectional schematic diagram of another embodiment of the medium arrangement pipe in the embodiment of the present disclosure is shown in FIG. Fig.21 In the example, the medium discharge pipe 433 has a bend. The medium discharge pipe 433 includes a vertical pipe section 4333 and an inclined pipe section 4334 which are connected to each other; the liquid inlet 4331 is formed at the top of the vertical pipe section 4333, and the end of the inclined pipe section 4334 extends to the outside of the collection chamber 4211 and forms the liquid discharge port 4332. It can be understood by those skilled in the art that the fewer bends the medium discharge pipe 433 has, the smaller the resistance of the continuous phase liquid when it flows inside the medium discharge pipe 433, and the better the effect of discharging the continuous phase liquid from the collection chamber 4211, and the inclined pipe section 4334 further improves the effect of discharging the continuous phase liquid from the collection chamber 4211.

[0111] In another embodiment, the medium discharge pipe 433 is implemented to include a vertical pipe section and an arc pipe section which are connected to each other; the arc opening of the arc pipe section faces upward.

[0112] In summary, the embodiments of the present disclosure provide a micro-droplet production device, including a continuous phase liquid supply component, a dispersed phase component, a micro-droplet cutting component and a current collecting component. The dispersed phase liquid supply component includes at least one injection pump, and the injection pump pipeline is connected to the dispersed phase container. The continuous phase liquid supply component, the pipeline is connected to the continuous phase container. The micro-droplet cutting component includes at least one microfluidic chip; the pipeline of the at least one microfluidic chip is connected to the injection pump to obtain the dispersed phase liquid, and the pipeline is connected to the continuous phase liquid supply component to obtain the continuous phase liquid. The current collecting component includes a confluence unit and a collection unit; the confluence unit pipeline is connected to the microfluidic chip, and the collection unit pipeline is connected to the confluence unit to collect micro-droplets. Through the cooperation of the dispersed phase liquid supply component, the continuous phase liquid supply component, the microfluidic chip and the current collecting component, the embodiments of the present disclosure can continuously supply the continuous phase liquid and the dispersed phase liquid to the microfluidic chip to increase the production rate of micro-droplets.

[0113] The above embodiments are merely illustrative of the principles and effects of the present disclosure, and are not intended to limit the present disclosure. Anyone familiar with the technology may modify or change the above embodiments without violating the spirit and scope of the present disclosure. Therefore, all equivalent modifications or changes made by those with ordinary knowledge in the technical field without departing from the spirit and technical ideas disclosed in the present disclosure shall still be covered by the protection scope of the present disclosure.

Claims

1. A micro-droplet production device, characterized in that: include: The dispersed phase liquid supply assembly comprises at least one injection pump; the injection pump pipeline is connected to the dispersed phase container; A continuous phase liquid supply assembly, the pipeline of which is connected to the continuous phase container; The micro-droplet cutting component comprises at least one microfluidic chip; the pipeline of the at least one microfluidic chip is connected to the injection pump to obtain the dispersed phase liquid, and the pipeline is connected to the continuous phase liquid supply component to obtain the continuous phase liquid; The current collecting component comprises a confluence unit and a collecting unit; the confluence unit pipeline is connected to the microfluidic chip, and the collecting unit pipeline is connected to the confluence unit to collect micro droplets.

2. The micro-droplet production device according to claim 1, characterized in that: The microfluidic chip comprises a continuous phase flow channel, a dispersed phase flow channel and a micro-droplet collecting chamber; the continuous phase flow channel is for loading a continuous phase liquid, and has a first inlet and a first outlet; the dispersed phase flow channel is located in the continuous phase flow channel, is for loading a dispersed phase liquid, and has a second inlet and a second outlet surrounded by the first outlet; a certain angle is formed between the flow direction of the dispersed phase flow channel and the continuous phase flow channel, so that the dispersed phase liquid is cut by the continuous phase liquid of the first outlet at the second outlet to form micro-droplets; the micro-droplet collecting chamber is connected to the first outlet and the second outlet.

3. The micro-droplet production device according to claim 2, characterized in that: At least one of the continuous phase flow channel and the dispersed phase flow channel is provided with a diameter-changing section whose inner diameter decreases along the flow direction.

4. The micro-droplet production device according to claim 1, characterized in that: The injection pump comprises a cavity for infusion and a heating component surrounding the cavity for infusion; a transfer assembly is provided between the injection pump and the microfluidic chip; the transfer assembly comprises at least one transfer joint, a continuous phase tube and a heat preservation tube; the transfer joint has a heat preservation cavity, and a first interface and a second interface connected to the heat preservation cavity; the first interface pipeline is connected to the heating component; one end of the heat preservation tube is connected to the second interface, and the other end is connected to the heating medium container; The continuous phase tube is arranged in the insulation tube, and one end of the continuous phase tube is connected to the microfluidic chip, and the other end of the continuous phase tube extends out of the insulation cavity after passing through the second interface and is connected to the cavity to be infused.

5. The micro-droplet production device according to claim 1, characterized in that: The injection pump comprises a cavity for infusion and a heating component surrounding the cavity for infusion; an adapter assembly is provided between the injection pump and the microfluidic chip; the adapter assembly comprises a first adapter, a second adapter, a continuous phase tube and a heat preservation tube; the first adapter has a first heat preservation cavity, and a first interface and a second interface connected to the first heat preservation cavity; the second adapter in a pair of adapters has a second heat preservation cavity, and a third interface and a fourth interface connected to the second heat preservation cavity; the first interface pipeline is connected to the heating component; one end of the heat preservation tube is connected to the second interface, and the other end is connected to the fourth interface; the third interface pipeline is connected to the heating medium container; The continuous phase tube is arranged in the insulation tube, and one end thereof extends out of the first insulation cavity after passing through the second interface and is connected to the cavity to be infused; The other end extends out of the second heat preservation cavity after passing through the fourth interface and is connected to the microfluidic chip.

6. The micro-droplet production device according to claim 1, characterized in that: It also includes a cleaning unit; the cleaning unit includes a cleaning pipe and at least one cleaning port connected to the cleaning pipe, and the at least one cleaning port pipeline is connected to the at least one injection pump.

7. The micro-droplet production device according to claim 1, characterized in that: It also includes a clean water unit; the clean water unit includes a clean water pipe and at least one clean water port connected to the clean water pipe, and the at least one clean water port pipeline is connected to the at least one injection pump.

8. The micro-droplet production device according to claim 1, characterized in that: The microfluidic chip is implemented as a plurality of chips; each of the plurality of microfluidic chips is connected to the at least one injection pump through a pipeline.

9. The micro-droplet production device according to claim 1, characterized in that: The injection pump and the microfluidic chip are both implemented in multiple numbers; each of the microfluidic chips is connected to one of the injection pumps through a pipeline.

10. The micro-droplet production device according to claim 1, characterized in that: The number of the injection pumps is not greater than the number of the microfluidic chips.

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