Microdroplet production apparatus

Abstract

The microdroplet production device comprises a continuous phase liquid supply assembly, a dispersed phase assembly, a microdroplet cutting assembly and a collection assembly. The dispersed phase assembly comprises at least one syringe pump, and the syringe pump is connected to a dispersed phase container in a pipeline manner. The continuous phase liquid supply assembly is connected to a continuous phase container in a pipeline manner. The microdroplet cutting assembly comprises at least one microfluidic chip, and the microfluidic chip is connected to the syringe pump in a pipeline manner to obtain the dispersed phase liquid and connected to the continuous phase liquid supply assembly in a pipeline manner to obtain the continuous phase liquid. The collection assembly comprises a flow converging unit and a collection unit, and the flow converging unit is connected to the microfluidic chip in a pipeline manner, and the collection unit is connected to the flow converging unit in a pipeline manner to collect the microdroplets. The microdroplet production device can continuously supply the continuous phase liquid and the dispersed phase liquid to the microfluidic chip through the cooperation of the dispersed phase assembly, the continuous phase liquid supply assembly, the microfluidic chip and the collection assembly, so as to improve the production rate of the microdroplets.

Description

Technical Field

[0001] This disclosure relates to the field of microdroplet production technology, and more particularly to microdroplet production equipment. Background Technology

[0002] Microdroplets have wide applications in various fields. Microfluidic technology based on microdroplets has seen rapid development and application in areas such as digital PCR, single-cell culture, single-cell genome / transcriptome sequencing, single-cell functional sorting, high-throughput reaction screening, and protein crystallization. Microdroplet generation utilizes two immiscible phases to form emulsified microdroplets; the microdroplet phase is called the dispersed phase, and the phase encapsulating the microdroplets is called the continuous phase. However, existing production equipment requires intermittent shutdowns when raw material replenishment is needed, often leading to batch-to-batch variations and equipment wear and tear. Furthermore, the stability of some solutions deteriorates during long-term storage, requiring reconfiguration based on storage conditions and cycles, further hindering the continuous production requirements of microfluidics and impeding its industrialization. Therefore, it is necessary to improve existing technologies to overcome their shortcomings. Summary of the Invention

[0003] In view of the shortcomings of the prior art described above, the purpose of this disclosure is to provide microdroplet production equipment to solve the problems in the related technology.

[0004] The first aspect of this disclosure provides a microdroplet production apparatus, comprising:

[0005] A dispersion phase supply assembly includes at least one injection pump; the injection pump is connected to the dispersion phase container.

[0006] Continuous phase liquid supply assembly, with pipelines connected to the continuous phase container;

[0007] A microdroplet cutting assembly includes at least one microfluidic chip; the at least one microfluidic chip is connected to the injection pump to obtain a dispersed phase liquid, and is also connected to the continuous phase liquid supply assembly to obtain a continuous phase liquid;

[0008] The current collection assembly includes a current collection unit and a current collection unit; the current collection unit is connected to the microfluidic chip via a conduit, and the current collection unit is connected to the current collection unit via a conduit to collect microdroplets.

[0009] In an embodiment of the first aspect, the microfluidic chip includes a continuous phase channel, a dispersed phase channel, and a microdroplet collection chamber; the continuous phase channel is for loading a continuous phase liquid and has a first inlet and a first outlet; the dispersed phase channel is located within the continuous phase channel and is for loading a dispersed phase liquid, having a second inlet and a second outlet surrounded by the first outlet; the flow directions of the dispersed phase channel and the continuous phase channel form a certain angle, so that the dispersed phase liquid is cut into microdroplets by the continuous phase liquid at the first outlet at the second outlet; the microdroplet collection chamber is connected to the first outlet and the second outlet.

[0010] In an embodiment of the first aspect, at least one of the continuous phase flow channel and the dispersed phase flow channel is provided with a variable diameter section whose inner diameter decreases along the flow direction.

[0011] In an embodiment of the first aspect, the injection pump includes an infusion chamber and a heating element surrounding the infusion chamber; a transition assembly is provided between the injection pump and the microfluidic chip; the transition assembly includes at least one adapter, a continuous phase tube, and a heat-insulating tube; the adapter has a heat-insulating cavity, and a first interface and a second interface communicating with the heat-insulating cavity; the first interface is connected to the heating element; one end of the heat-insulating tube is connected to the second interface, and the other end is connected to a heating medium container; the continuous phase tube is disposed inside the heat-insulating tube, and one end is connected to the microfluidic chip, and the other end extends out of the heat-insulating cavity and communicates with the infusion chamber after passing through the second interface.

[0012] In a first aspect embodiment, the injection pump includes an infusion chamber and a heating element surrounding the infusion chamber; a transition assembly is provided between the injection pump and the microfluidic chip; the transition assembly includes a first adapter, a second adapter, a continuous phase tube, and a heat-insulating tube; the first adapter has a first heat-insulating cavity, and a first interface and a second interface communicating with the first heat-insulating cavity; the second adapter of the pair of adapters has a second heat-insulating cavity, and a third interface and a fourth interface communicating with the second heat-insulating cavity; the first interface is connected to the heating element; one end of the heat-insulating tube is connected to the second interface, and the other end is connected to the fourth interface; the third interface is connected to a heating medium container; the continuous phase tube is disposed inside the heat-insulating tube, and one end extends out of the first heat-insulating cavity and communicates with the infusion chamber after passing through the second interface; the other end extends out of the second heat-insulating cavity and communicates with the microfluidic chip after passing through the fourth interface.

[0013] In an embodiment of the first aspect, a cleaning unit is further included; the cleaning unit includes a cleaning tube and at least one cleaning port connected to the cleaning tube, the at least one cleaning port being connected to the at least one injection pump.

[0014] In an embodiment of the first aspect, a water purification unit is further included; the water purification unit includes a water purification pipe and at least one water purification outlet connected to the water purification pipe, the at least one water purification outlet being connected to the at least one injection pump.

[0015] In an embodiment of the first aspect, the microfluidic chip is implemented as a plurality of chips; all of the plurality of microfluidic chips are connected in tubing to the at least one injection pump.

[0016] In an embodiment of the first aspect, both the injection pump and the microfluidic chip are implemented as a plurality; each of the microfluidic chips is connected in a conduit to one of the injection pumps.

[0017] In an embodiment of the first aspect, the number of injection pumps is no greater than the number of microfluidic chips.

[0018] As described above, embodiments of this disclosure provide a microdroplet production apparatus, including a continuous phase supply component, a dispersed phase component, a microdroplet cutting component, and a collection component. The dispersed phase supply component includes at least one injection pump, the injection pump being connected to a dispersed phase container. The continuous phase supply component is also connected to the continuous phase container. The microdroplet cutting component includes at least one microfluidic chip; the at least one microfluidic chip is connected to the injection pump to obtain dispersed phase liquid, and is also connected to the continuous phase supply component to obtain continuous phase liquid. The collection component includes a manifold unit and a collection unit; the manifold unit is connected to the microfluidic chip, and the collection unit is connected to the manifold unit to collect microdroplets. By coordinating the dispersed phase supply component, the continuous phase supply component, the microfluidic chip, and the collection component, embodiments of this disclosure can continuously supply continuous and dispersed phase liquids to the microfluidic chip, thereby improving the microdroplet production rate. Attached Figure Description

[0019] Figure 1 The diagram shown is a structural connection diagram of the microdroplet production equipment in an embodiment of this disclosure;

[0020] Figure 2 The diagram shown is a schematic representation of the overall structure of the microdroplet production equipment in an embodiment of this disclosure.

[0021] Figure 3 The diagram shown is a cross-sectional view of the overall structure of the adapter components in an embodiment of this disclosure.

[0022] Figure 4 The following is an embodiment of this disclosure. Figure 3 Enlarged view of A in the middle;

[0023] Figure 5 The diagram shown is a schematic diagram of the overall structure of the adapter components in another embodiment of this disclosure;

[0024] Figure 6 The diagram shown is a schematic diagram of a microfluidic chip in an embodiment of this disclosure;

[0025] Figure 7 The illustration shown is an embodiment of this disclosure. Figure 6 Enlarged view of B in the middle;

[0026] Figure 8 The diagram shown is a cross-sectional view of the disassembly and connection of the microfluidic chip in an embodiment of this disclosure;

[0027] Figure 9 The diagram shown is a schematic diagram of the overall structure of the injection pump in an embodiment of this disclosure;

[0028] Figure 10 The diagram shown is a cross-sectional view of the overall structure of the injection pump in an embodiment of this disclosure;

[0029] Figure 11 The figure shown is a cross-sectional schematic diagram of the first embodiment of the serpentine heating channel in this disclosure;

[0030] Figure 12 The diagram shown is a schematic diagram of the overall structure of the first embodiment of the serpentine heating channel in this disclosure;

[0031] Figure 13 The figure shown is a cross-sectional schematic diagram of the second embodiment of the heating flow channel with a serpentine shape in this disclosure;

[0032] Figure 14 The diagram shown is a schematic representation of the overall structure of the second embodiment of the serpentine heating channel in this disclosure.

[0033] Figure 15 The diagram shown is a schematic representation of the second embodiment of the serpentine heating channel in this disclosure.

[0034] Figure 16 The diagram shown is a cross-sectional schematic of the overall structure in which the heating element is implemented as a heating wire in this disclosure;

[0035] Figure 17 The diagram shown is a cross-sectional view of the overall structure of the collection unit in an embodiment of this disclosure;

[0036] Figure 18 The diagram shown is a cross-sectional view of the collection unit from another perspective in an embodiment of this disclosure;

[0037] Figure 19 The diagram shown is a cross-sectional view of an embodiment of this disclosure, including a liquid collection tube.

[0038] Figure 20 The diagram shown is a cross-sectional view of the liquid collection tube from another perspective in an embodiment of this disclosure;

[0039] Figure 21 The diagram shown is a cross-sectional view of another embodiment of the media pipe in this disclosure.

[0040] Figure label:

[0041] 100. Dispersed phase supply assembly; 110. Injection pump; 111. Injection pump body; 1111. Infusion chamber; 11111. Injection port; 11112. Infusion port; 1112. Piston block; 1113. Sealing plug; 1114. Insulation layer; 112. Heating component; 1121. Heating channel; 11211. Second outlet; 11212. Second inlet; 1122. Heating wire; 120. Dispersed phase container; 130. Cleaning unit; 131. Cleaning pipe; 1301. Cleaning port; 140. Clean water unit; 141. Clean water pipe; 1401. Clean water outlet;

[0042] 200. Continuous phase liquid supply assembly; 210. Continuous phase container; 220. Continuous phase liquid supply pipe;

[0043] 300, Microfluidic chip; 310, First substrate; 320, Second substrate; 321, Second block; 322, Liquid guide tube; 323, Seal; 301, Continuous phase flow channel; 3011, First inlet; 3012, First outlet; 3013, Second diameter changing section; 302, Dispersed phase flow channel; 3021, Second inlet; 3022, Second outlet; 3023, First diameter changing section; 303, Microdroplet collection chamber; 3031, Guide section; 3032, Third outlet;

[0044] 400. Combination assembly; 410. Merging unit; 420. Collection unit; 421. Collection container; 4211. Collection chamber; 42111. First channel; 42112. Second channel; 4212. Top opening; 4213. Collection cylinder; 4214. Cover plate; 4215. Bottom opening; 4216. Guide section; 422. Partition plate; 433. Medium pipe; 4331. Liquid inlet; 4332. Liquid outlet; 4333. Vertical pipe section; 4334. Inclined pipe section; 434. Collection pipe.

[0045] 500, Adapter assembly; 510, Adapter; 5101, Insulation cavity; 5102, First interface; 5103, Second interface; 520, Continuous phase tube; 530, Insulation tube; 500A, Adapter assembly; 510A, First adapter; 5101A, First insulation cavity; 5102A, Third interface; 5103A, Fourth interface; 510B, Second adapter; 5101B, Second insulation cavity; 5102B, Fifth interface; 5103B, Sixth interface. Detailed Implementation

[0046] The following specific examples illustrate the implementation of this disclosure. Those skilled in the art can easily understand other advantages and effects of this disclosure from the information disclosed herein. This disclosure can also be implemented or applied through other different specific embodiments, and various details in this disclosure can be modified or changed according to different viewpoints and application modules without departing from the spirit of this disclosure. It should be noted that, unless otherwise specified, the embodiments and features in the embodiments of this disclosure can be combined with each other.

[0047] The embodiments of this disclosure will now be described in detail with reference to the accompanying drawings, so that those skilled in the art to which this disclosure pertains can readily implement it. This disclosure may be embodied in many different forms and is not limited to the embodiments described herein.

[0048] In this disclosure, references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic represented in connection with that embodiment or example is included in at least one embodiment or example of this disclosure. Furthermore, the specific features, structures, materials, or characteristics represented may be combined in any suitable manner in any one or a group of embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples represented in this disclosure, as well as the features of those different embodiments or examples.

[0049] Furthermore, the terms "first" and "second" are used for illustrative purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the representation of this disclosure, "a set" means two or more, unless otherwise explicitly specified.

[0050] For the purpose of clarity, devices unrelated to the description are omitted, and the same or similar components throughout the specification are given the same reference numerals.

[0051] Throughout this specification, when it is said that a device is "connected" to another device, this includes not only "direct connection" but also "indirect connection" by placing other components in between. Furthermore, when it is said that a device "comprises" a certain constituent element, unless otherwise stated otherwise, this does not exclude other constituent elements, but rather implies that other constituent elements may be included.

[0052] While the terms first, second, etc., are used in some examples herein to refer to various elements, these elements should not be limited by these terms. These terms are used only to distinguish one element from another. For example, first interface and second interface, etc., are used. Furthermore, as used herein, the singular forms “a,” “an,” and “the” are intended to also include the plural forms unless the context indicates otherwise. It should be further understood that the terms “comprising,” “including,” indicate the presence of the stated feature, step, operation, element, module, item, kind, and / or group, but do not exclude the presence, occurrence, or addition of one or more other features, steps, operations, elements, modules, items, kinds, and / or groups. The terms “or” and “and / or” as used herein are interpreted as inclusive, or mean any one or any combination thereof. Thus, “A, B, or C” or “A, B, and / or C” means “any one of: A; B; C; A and B; A and C; B and C; A, B, and C.” Exceptions to this definition will only occur if the combination of elements, functions, steps, or operations is inherently mutually exclusive in some way.

[0053] The technical terms used herein are for reference only to specific embodiments and are not intended to limit the scope of this disclosure. The singular form used herein includes the plural form unless the statement explicitly indicates otherwise. The word "comprising" as used in this specification means to specify a particular characteristic, region, integer, step, operation, element, and / or component, and does not exclude the presence or addition of other characteristics, regions, integers, steps, operations, elements, and / or components.

[0054] Although not explicitly defined, all terms, including technical and scientific terms used herein, shall have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure pertains. Terms defined in commonly used dictionaries shall be further interpreted as having a meaning consistent with the relevant technical literature and the message of the present disclosure, and shall not be over-interpreted as having an ideal or overly formulaic meaning unless otherwise defined.

[0055] Microdroplets have wide applications in various fields. Microfluidic technology based on microdroplets has seen rapid development and application in areas such as digital PCR, single-cell culture, single-cell genome / transcriptome sequencing, single-cell functional sorting, high-throughput reaction screening, and protein crystallization. Microdroplet generation utilizes two immiscible phases to form emulsified microdroplets; the microdroplet phase is called the dispersed phase, and the phase encapsulating the microdroplets is called the continuous phase. However, existing production equipment requires intermittent shutdowns when raw material replenishment is needed, often leading to batch-to-batch variations and equipment wear and tear. Furthermore, the stability of some solutions deteriorates during long-term storage, requiring reconfiguration based on storage conditions and cycles, further hindering the continuous production requirements of microfluidics and impeding its industrialization. Therefore, it is necessary to improve existing technologies to overcome their shortcomings.

[0056] Based on the above problems, the embodiments of this disclosure, through the cooperation of the dispersed phase liquid supply component, the continuous phase liquid supply component, the microfluidic chip and the current collection component, can continuously supply continuous phase liquid and dispersed phase liquid to the microfluidic chip, thereby improving the production rate of microdroplets.

[0057] Figure 1 The diagram shown is a structural connection diagram of the microdroplet production equipment in an embodiment of this disclosure. Figure 2 The diagram shown is a schematic representation of the overall structure of the microdroplet production equipment in an embodiment of this disclosure. Figure 1 and Figure 2 In this example, the microdroplet production equipment includes a dispersed phase supply assembly 100, a continuous phase supply assembly 200, a microdroplet cutting assembly, and a collection assembly 400. The dispersed phase supply assembly 100 includes at least one injection pump 110, which is connected to a dispersed phase container 120. The continuous phase supply assembly 200 is connected to a continuous phase container 210. The microdroplet cutting assembly includes at least one microfluidic chip 300; the at least one microfluidic chip 300 is connected to the injection pump 110 to obtain the dispersed phase liquid, and is also connected to the continuous phase supply assembly 200 to obtain the continuous phase liquid. The collection assembly 400 includes a manifold unit 410 and a collection unit 420; the manifold unit 410 is connected to the microfluidic chip 300, and the collection unit 420 is connected to the manifold unit 410 to collect microdroplets.

[0058] The advantage of the above configuration is that, through the cooperation of the dispersed phase liquid supply component 100, the continuous phase liquid supply component 200, the microfluidic chip 300 and the flow collection component 400, the continuous phase liquid and the dispersed phase liquid can be continuously supplied to the microfluidic chip 300, thereby improving the production rate of microdroplets.

[0059] Exemplarily, both the syringe pump 110 and the microfluidic chip 300 are implemented in multiples; each microfluidic chip 300 is connected via tubing to one of the syringe pumps 110. Preferably, the number of syringe pumps 110 is no greater than the number of microfluidic chips 300. For example, the number of microfluidic chips 300 is an integer multiple of the number of syringe pumps 110. That is, one syringe pump 110 simultaneously supplies dispersed phase liquid to multiple microfluidic chips 300 via tubing.

[0060] In another embodiment, only a plurality of microfluidic chips 300 are implemented; each of the plurality of microfluidic chips 300 is connected in tubing to the at least one injection pump 110. For example, the at least one injection pump 110 has a plurality of infusion ports, and each of the microfluidic chips 300 is connected in tubing to one of the infusion ports.

[0061] Figure 3 The diagram shown is a cross-sectional view of the overall structure of the adapter assembly in an embodiment of this disclosure. Figure 2 and Figure 3 In the example, the microdroplet production equipment further includes a cleaning unit 130; the cleaning unit 130 includes a cleaning tube 131 and at least one cleaning port 1301 connected to the cleaning tube 131, the at least one cleaning port 1301 being connected to the at least one injection pump 110. As another example, the microdroplet production equipment further includes a water purification unit 140; the water purification unit 140 includes a water purification tube 141 and at least one water purification port 1401 connected to the water purification tube 141, the at least one water purification port 1401 being connected to the at least one injection pump 110. The advantage of this configuration is that the cleaning unit 130 injects cleaning fluid into the injection pump 110 to clean the injection pump 110, and then the water purification unit 140 rinses away any residual cleaning fluid in the injection pump 110. This avoids a decrease in the microdroplet yield due to the injection pump 110 not being cleaned in a timely manner.

[0062] For example, the cleaning port 1301 and the clean water port 1401 are configured to communicate with the same opening of the syringe pump 110; they can also be configured to communicate with different openings of the syringe pump 110.

[0063] For example, the manifold unit 410 is implemented as a plurality of manifolds (not shown in the figure), the number of which matches the number of microfluidic chips 300; the first port of each manifold is connected to one of the microfluidic chips 300, and the second ports of the plurality of manifolds can be directly and individually connected to the collection unit 420, or they can be connected to the collection unit 420 in parallel. In another embodiment, the manifold unit 410 is implemented as a connector to connect the microfluidic chip 300 and the collection unit 420.

[0064] exist Figure 3 In the example, the continuous phase supply assembly 200 is implemented to include at least one continuous phase supply tube 220, one end of which is connected to the continuous phase container 210 and the other end of which is connected to the microfluidic chip 300. Further exemplarily, when multiple microfluidic chips 300 are implemented, the number of continuous phase supply tubes 220 is matched to the number of microfluidic chips 300.

[0065] Figure 4 The illustration shown is an embodiment of this disclosure. Figure 3 A magnified view of A in the diagram. Figure 3 and Figure 4 In the example, the syringe pump 110 includes an infusion chamber 1111 and a heating element 112 surrounding the infusion chamber 1111, wherein the heating element 112 is implemented to heat a stairwell 1121. A transition assembly 500 is provided between the syringe pump 110 and the microfluidic chip 300; the transition assembly 500 includes at least one adapter 510, a dispersion phase tube 520, and a heat insulation tube 530. Exemplarily, the adapter 510 is detachably disposed on the syringe pump 110, for example, by snap-fit ​​or screw connection. The adapter 510 has a heat-insulating cavity 5101, and a first interface 5102 and a second interface 5103 connecting the heat-insulating cavity 5101; the first interface 5102 is connected to the heating component 112; one end of the heat-insulating tube 530 is connected to the second interface 5103, and the other end is connected to the heating medium container (not shown in the figure); the dispersed phase tube 520 is disposed inside the heat-insulating tube 530, and one end is connected to the microfluidic chip 300, and the other end extends out of the heat-insulating cavity 5101 after passing through the second interface 5103 and is connected to the infusion chamber 1111. The advantage of the above configuration is that it can keep the dispersed phase liquid flowing out of the syringe pump 110 warm, avoiding the situation where the dispersed phase liquid solidifies due to heat loss during transportation.

[0066] Figure 5 The diagram shown is a schematic representation of the overall structure of the adapter assembly in another embodiment of this disclosure. Figure 5In the example, the injection pump 110 includes an infusion chamber 1111 and a heating element 112 surrounding the infusion chamber 1111; an adapter assembly 500A is provided between the injection pump 110 and the microfluidic chip 300; the adapter assembly 500 includes a first adapter 510A, a second adapter 510B, a dispersion phase tube 520, and a heat insulation tube 530; the first adapter 510A has a first heat insulation chamber 5101A, and a third interface 5102A and a fourth interface 5103A communicating with the first heat insulation chamber 5101A; the second adapter 510B of the pair of adapters has a second heat insulation chamber 5101B, and communicates with the second heat insulation chamber 5101B. The fifth interface 5104B and the sixth interface 5105B are connected; the third interface 5102A is connected to the heating component 112; one end of the insulation tube 530 is connected to the fourth interface 5103A, and the other end is connected to the sixth interface 5105B; the fifth interface 5104B is connected to the heating medium container (not shown in the figure); the dispersed phase tube 520 is disposed inside the insulation tube 530, and one end extends out of the first insulation cavity 5101A after passing through the fourth interface 5103A and is connected to the infusion chamber 1111; the other end extends out of the second insulation cavity 5101B after passing through the sixth interface 5105B and is connected to the microfluidic chip 300. This embodiment can further avoid the situation where the dispersed phase liquid solidifies due to heat loss during transportation.

[0067] Figure 6 The diagram shown is a schematic diagram of a microfluidic chip in an embodiment of this disclosure. Figure 7 The illustration shown is an embodiment of this disclosure. Figure 6 A magnified view of B in the image. Figure 6 and Figure 7 In this example, the microfluidic chip 300 includes a continuous phase flow channel 301, a dispersed phase flow channel 302, and a microdroplet collection chamber 303. The continuous phase flow channel 301 is for loading a continuous phase liquid and has a first inlet 3011 and a first outlet 3012. The dispersed phase flow channel 302 is located within the continuous phase flow channel 301 and is for loading a dispersed phase liquid. It has a second inlet 3021 and a second outlet 3022 surrounded by the first outlet 3012. The dispersed phase flow channel 302 forms an angle with the flow direction of the continuous phase flow channel 301, so that the dispersed phase liquid is cut into microdroplets at the second outlet 3022 by the continuous phase liquid at the first outlet 3012. The microdroplet collection chamber 303 is connected to the first outlet 3012 and the second outlet 3022. It is understood that the continuous phase supply pipe is connected to the continuous phase flow channel 301, and the dispersed phase supply pipe is connected to the dispersed phase flow channel 302.

[0068] The advantage of the above arrangement is that the continuous phase flow channel 301 arranged in a ring can form a microdroplet collection cavity 303 surrounding the second outlet 3022. The continuous phase liquid in the microdroplet collection cavity 303 can encapsulate a large number of dispersed phase droplets flowing out from the second outlet 3022, solving the problem of slow microdroplet production rate caused by insufficient supply of the continuous phase liquid and increasing the output of microdroplets.

[0069] Microspheres, as a type of microdroplet, have wide applications in many fields, including drug delivery, chemical separation, catalytic reactions, and biomedical applications.

[0070] exist Figure 6 and Figure 7 In the example, the region within the dispersed phase flow channel 302 that is at least surrounded by the continuous phase flow channel 301 is implemented as a first variable diameter section 3023, the inner diameter of which decreases along the flow direction. It is understood that as the dispersed phase liquid flows through the first variable diameter section 3023, the flow velocity of the dispersed phase liquid within the dispersed phase flow channel 302 is increased as the inner diameter gradually decreases along the flow direction. This increases the velocity at which the dispersed phase liquid exits the second outlet 3022 and forms dispersed phase droplets, thereby increasing the yield of microdroplets.

[0071] exist Figure 6 and Figure 7 In the example, the continuous phase flow channel 301 is configured as a second variable diameter section 3013, at least surrounding a portion of the dispersed phase flow channel 302. The inner diameter of the second variable diameter section 3013 decreases along the flow direction. It is understood that as the continuous phase liquid flows through the second variable diameter section 3013, the flow velocity of the continuous phase liquid within the continuous phase flow channel 301 is increased as the inner diameter gradually decreases along the flow direction. This increases the velocity of the continuous phase liquid flowing into the microdroplet collection chamber 303 and allows it to accumulate there in large quantities. This, in turn, enables the encapsulation of a large number of dispersed phase droplets flowing out from the second outlet 3022, preventing the problem of insufficient continuous phase liquid supply leading to the inability of microdroplets to form, and further increasing the yield of microdroplets.

[0072] Exemplarily, the first variable diameter section 3023 and the second variable diameter section 3013 can be implemented individually or simultaneously. In this embodiment, the first variable diameter section 3023 and the second variable diameter section 3013 are implemented simultaneously. Those skilled in the art will understand that the larger the angle between the second variable diameter section 3013 and the liquid flow direction, the greater the cutting force exerted by the continuous phase liquid flowing from the first outlet 3012 on the dispersed phase liquid flowing from the second outlet 3022 along the direction perpendicular to the liquid flow direction. That is, the cutting effect on the dispersed phase liquid flowing from the second outlet 3022 is better, thereby significantly improving the production rate of microdroplets.

[0073] For example, the inner diameter of the microdroplet collection cavity 303 is larger than the inner diameter of the second outlet 3022. It is understood that the larger inner diameter of the microdroplet collection cavity 303 allows it to accommodate a large amount of continuous phase liquid at the second outlet 3022, thereby enabling the encapsulation of a large number of dispersed phase droplets flowing out of the second outlet 3022 and preventing the problem of insufficient continuous phase liquid supply leading to the inability of microdroplets to form.

[0074] Exemplarily, the first outlet 3012, the second outlet 3022, and the microdroplet collection cavity 303 are coaxial. Preferably, the cross-section of the microdroplet collection cavity 303 is circular. That is, the second outlet 3022 is aligned with the first outlet 3012 and the microdroplet collection cavity 303. This arrangement has the advantage of accelerating the flow rate of finished microdroplets formed within the microdroplet collection cavity 303 out of the first outlet 3012, preventing the accumulation of finished microdroplets within the microdroplet collection cavity 303, and reducing the microdroplet production rate. In another embodiment, the cross-section of the microdroplet collection cavity 303 may also be rectangular.

[0075] For example, the first inlet 3011 may also be implemented as two or more. When the first inlet 3011 is implemented as two, the two first inlets 3011 are symmetrically arranged. When the first inlet 3011 is implemented as multiple, the multiple first inlets 3011 are circumferentially spaced and uniformly distributed, so as to make the pressure of the continuous phase liquid in the continuous phase flow channel 301 more uniform.

[0076] exist Figure 7 In the example, the microdroplet collection chamber 303 includes a third outlet 3032 for discharging microdroplets. The aperture of the third outlet 3032 is smaller than the inner diameter of the microdroplet collection chamber 303. It is understood that the small aperture of the third outlet 3032 can both reduce the amount of continuous phase liquid flowing out through the third outlet 3032, avoiding waste of the continuous phase liquid, and allow the finished microdroplets to flow out.

[0077] For example, a tapered guide portion 3031 is provided at the connection between the microdroplet collection cavity 303 and the third outlet 3032. The advantage of this design is that it guides the microdroplets within the microdroplet collection cavity 303 towards the third outlet 3032, further preventing the accumulation of microdroplets within the microdroplet collection cavity 303 and the blockage of the third outlet 3032.

[0078] As a further example, the distance between the second outlet 3022 and the third outlet 3032 is adjustable, and the diameter of the microdroplets increases as the distance between the second outlet 3022 and the third outlet 3032 increases. Those skilled in the art will understand that, firstly, the greater the distance between the second outlet 3022 and the third outlet 3032, the smaller the component force (cutting force) perpendicular to the flow direction of the continuous phase liquid on the dispersed phase liquid flowing out of the second outlet 3022 when it flows in the microdroplet collection chamber 303, resulting in the dispersed phase liquid flowing out of the second outlet 3022 not being quickly cut into droplets; secondly, the greater the distance between the second outlet 3022 and the third outlet 3032, the longer the path for the dispersed phase liquid flowing out of the second outlet 3022 to combine with the external continuous phase liquid, ultimately resulting in a larger volume of dispersed phase droplets flowing out of the second outlet 3022 and being cut into them, and the diameter of the final product's microdroplets increases accordingly, and vice versa, the diameter of the final product's microdroplets decreases accordingly, so this will not be elaborated further here.

[0079] Figure 8 The diagram shown is a cross-sectional view illustrating the disassembly and connection of a microfluidic chip in an embodiment of this disclosure. Figure 8 In the example, the continuous phase flow channel 301 is formed on the first substrate 310; the dispersed phase flow channel 302 is formed on the second substrate 320; the first substrate 310 and the second substrate 320 are detachably connected, and the continuous phase flow channel 301 and the dispersed phase flow channel 302 are connected after the first substrate 310 is connected to the second substrate 320.

[0080] Exemplarily, the second substrate 320 includes a detachably connected second base block 321 and a liquid guide tube 322, and the dispersed phase flow channel 302 is formed in the second base block 321 and the liquid guide tube 322; the wall surface of the first substrate 310 that is in contact with the second substrate 320 is provided with an insertion cavity (attached). Figure 6 The liquid guide tube 322 is inserted into the area described above. When the first base 310 is connected to the second base 320, the liquid guide tube 322 is inserted into the insertion cavity to form the continuous phase flow channel 301 surrounding the dispersed phase flow channel 302. In this embodiment, the second base block 321 and the liquid guide tube 322 are threaded together. In another embodiment, the disassembly and connection method of the second base block 321 and the liquid guide tube 322 is implemented by first engaging (positioning) and then bolting (fastening).

[0081] As a further example, the second base block 321 is provided with a second sub-channel including a second sub-inlet and a second sub-outlet, and the first end of the liquid guide tube 322 is threadedly connected to the second sub-outlet. That is to say, the dispersed phase flow channel 302 is jointly composed of the second sub-channel and the inner cavity of the liquid guide tube 322, and the second outlet 3022 is formed at the second end of the liquid guide tube 322.

[0082] exist Figure 8 In this embodiment, the distance between the second outlet 3022 and the third outlet 3032 can be adjusted by adjusting the length of the liquid guide tube 322 screwed into the second sub-outlet.

[0083] Those skilled in the art will understand that the disassembly and connection of the first substrate 310, the second substrate 320, and the liquid guide tube 322 not only facilitates the replacement of the liquid guide tube 322 with different inner diameters to produce microdroplets of different diameters, but also facilitates the periodic cleaning of the continuous phase flow channel 301, the dispersed phase flow channel 302, and the inside of the liquid guide tube 322 to maintain the cleanliness of the chip interior.

[0084] For example, the first base 310 and the second base 320 are connected by bolts. For instance, the first base 310 has a first threaded hole, and the second base 320 has a second threaded hole. In another embodiment, the first base 310 and the second base 320 are connected by snap-fit.

[0085] exist Figure 8 In the example, a seal 323 is provided between the wall surfaces of the first substrate 310 and the second substrate 320 that are in contact with each other, so as to form a seal on the continuous phase flow channel 301 and the dispersed phase flow channel 302 after the first substrate 310 and the second substrate 320 are connected. Exemplarily, the wall surface of the second substrate 320 that is in contact with the first substrate 310 is provided with an annular sealing groove surrounding the liquid guide tube 322, and the seal 323, implemented as a sealing ring, is filled in the annular sealing groove. Further exemplaryly, the insertion cavity is located within the annular sealing groove. It is understood that the seal 323 can improve the sealing effect on the continuous phase flow channel 301 after the first substrate 310 and the second substrate 320 are connected, preventing liquid in the continuous phase flow channel 301 and the dispersed phase flow channel 302 from flowing out from the gap between the first substrate 310 and the second substrate 320.

[0086] Figure 9 The diagram shown is a schematic diagram of the overall structure of the injection pump in an embodiment of this disclosure. Figure 10 The diagram shown is a cross-sectional view of the overall structure of the injection pump in an embodiment of this disclosure. Figure 9 and Figure 10In the example, the syringe pump 110 includes a syringe pump body 111 and a heating element 112. The syringe pump body 111 includes a cavity 1111 for loading a continuous phase liquid. The heating element 112 extends along the cavity 1111 and is disposed on the syringe pump body 111 in a covering manner over the cavity 1111.

[0087] The advantage of the above configuration is that the heating component 112 can not only perform the basic function of heating the dispersed liquid in the infusion chamber 1111, but also improve the heating effect of the dispersed liquid in the infusion chamber 1111 through the heating medium flowing from bottom to top in the heating component 112.

[0088] For example, the heating element 112 is provided on the outer wall or inside the wall of the injection pump body 111.

[0089] exist Figure 10 In this example, the heating element 112 is disposed on the outer wall of the syringe pump body 111. The heating element 112 forms a heating channel 1121 for loading a heating medium; the heating channel 1121 has a second liquid outlet 11211 at the top and a second liquid inlet 11212 at the bottom. Exemplarily, the heating medium is implemented as oil or water.

[0090] exist Figure 10 In the example, the syringe pump body 111 further includes an injection port 11111 and an infusion port 11112 for communication with the infusion chamber 1111; the injection port 11111 and the second outlet port 11211 are formed on the same wall surface of the syringe pump body 111. For example, in this embodiment, the injection port 11111 and the infusion port 11112 are located on the top wall of the syringe pump body 111. In another embodiment, the injection port 11111 and the infusion port 11112 are located on the bottom wall of the syringe pump body 111.

[0091] In this embodiment, the injection pump 110 further includes a heating element (not shown in the figure), which is arranged around the injection pump body 111 in relation to the region of the heating channel 1121 near the second outlet 11211. Exemplarily, the heating element is implemented as a heating plate. Those skilled in the art will understand that as the heating medium flows within the heating channel 1121, its temperature gradually decreases (i.e., the closer to the second outlet 11211, the lower the temperature of the heating medium). Therefore, the heating element located near the region of the second outlet 11211 can provide auxiliary heating to the dispersed phase liquid corresponding to the region of the second outlet 11211, thereby increasing the temperature of the dispersed phase liquid at the infusion port 11112 and improving the temperature uniformity of the dispersed phase liquid within the infusion chamber 1111.

[0092] In another embodiment, the injection port 11111 and the infusion port 11112 may also be formed on the bottom wall of the injection pump body 111.

[0093] Exemplarily, the syringe pump body 111 further includes a piston block 1113 disposed in the infusion chamber 1111, which can be close to or away from the infusion port 11112. It is understood that when the piston block 1113 is close to the infusion port 11112, it squeezes the dispersed phase liquid out of the infusion chamber 1111 through the infusion port 11112; when the piston block 1113 is away from the infusion port 11112, it adds the dispersed phase liquid to the infusion chamber 1111 through the injection port 11111.

[0094] Exemplarily, the syringe pump body 111 further includes a sealing plug 1112 detachably connected to the main liquid inlet 1011. For example, the liquid inlet 11111 has an internal thread, and the outer surface of the cylindrical body of the sealing plug 1112 has an external thread that engages with the internal thread. Further exemplary, a sealing ring (not shown in the figure) is provided between the sealing plug 1112 and the syringe pump body 111.

[0095] For example, the heating channel 1121 is serpentinely arranged around the injection pump body 111.

[0096] Figure 11 The diagram shown is a cross-sectional schematic of the first embodiment of the serpentine heating channel in this disclosure. Figure 12 The diagram shown is a schematic representation of the overall structure of the first embodiment of the serpentine heating channel in this disclosure. Figure 11 and Figure 12 In the example, the heating element 112, implemented as the heating channel 1121, is disposed within the wall of the syringe pump body 111. The heating channel 1121 is configured to spirally surround the syringe pump body 111 along its axial direction. Exemplarily, the spiral direction can be either clockwise or counterclockwise. Further exemplaryly, the pitch of the spiral heating channel is configured such that the spacing between adjacent heating channels 1121 is sufficiently small to increase the coverage area of ​​the heating channel 1121 over the infusion chamber 1111. The advantage of this configuration is that, while ensuring the coverage area of ​​the heating channel 1121 over the infusion chamber 1111, the flow time of the heating medium within the heating channel 1121 is extended, thereby further improving the heating effect of the heating medium on the dispersed liquid in the infusion chamber 1111.

[0097] Figure 13 The diagram shown is a cross-sectional schematic of the second embodiment of the serpentine heating channel in this disclosure. Figure 14 The diagram shown is a schematic diagram of the overall structure of the second embodiment of the serpentine heating channel in this disclosure. Figure 15 The diagram shown is a schematic representation of the second embodiment of the serpentine heating channel in this disclosure. Figure 13 , 14 and Figure 15 In the example, the heating element, implemented as the heating channel 1121, is disposed within the wall of the injection pump body 111. The heating channel 1121 is configured to serpentinely surround the injection pump body 111 circumferentially. Those skilled in the art will understand that, in this embodiment, the heating medium flows upward along the circumference of the injection pump body 111 to the top of the injection pump body 111, then downward to the bottom of the injection pump body 111, and then repeats the above process.

[0098] In this way, the heating medium can flow back and forth between the top and bottom of the injection pump body 111, alternately transferring heat to the top and bottom of the injection pump body 111. This avoids the situation where heat transfer of the heating medium can only go from bottom to top, resulting in heat attenuation at the top, and improves the uniform heating of the dispersed liquid in the infusion chamber 1111 by the heating medium. Secondly, the heating effect of the heating medium on the dispersed liquid in the infusion chamber 1111 can be further improved by extending the flow time of the heating medium in the heating channel 1121.

[0099] Figure 16 The diagram shown is a cross-sectional schematic of the overall structure in which the heating element is implemented as a heating wire in this disclosure. Figure 16 In this example, the heating element 112 is implemented as a heating wire 1122, which is serpentinely wrapped around the syringe pump body 111 along its axial direction. For example, the winding method of the heating wire 1122 is similar to... Figure 12 The winding method is the same in the examples.

[0100] In the above embodiment, the injection pump 110 further includes a heat insulation layer 1114. The heat insulation layer 1114 covers the annular sidewall of the injection pump body 111. Exemplarily, the heat insulation layer 1114 is implemented as heat insulation cotton or an inert gas layer. The advantage of this configuration is that it can reduce the heat loss of the dispersed liquid in the infusion chamber 1111 and reduce the heat transfer between the heating medium and the outside environment.

[0101] Figure 17 The diagram shown is a cross-sectional view of the overall structure of the collection unit in an embodiment of this disclosure. Figure 18 The diagram shown is a cross-sectional view of the collection unit from another perspective in an embodiment of this disclosure. Figure 17 and Figure 18 In the example, the collection unit 420 includes a collection container 421, a partition 422, and a media outlet 433. The collection container 421 includes a collection cavity 4211 with a top opening 4212. The partition 422 is vertically disposed in the collection cavity 4211 to separate a portion of the space of the collection cavity 4211 into a first channel 42111 and a second channel 42112, which are separated from each other from the top opening 4212; wherein the first channel 42111 is connected to the top opening 4212; and the top opening 4212 allows a medium containing microdroplets to enter. The media outlet 433 includes a liquid inlet 4331 disposed in the second channel 42112 and extends to the outside of the collection cavity 4211.

[0102] The advantage of the above configuration is that the partition 422 separates the first channel 42111 from the collection chamber 4211 and the liquid inlet 4331 of the medium drain pipe 433, thus preventing microdroplets entering the collection chamber 4211 from entering the liquid inlet 4331 and being mistakenly discharged from the collection chamber 4211. This indirectly increases the production of microdroplets by avoiding waste.

[0103] For example, the distance between the inlet 4331 and the top opening 4212 is smaller than the distance between the bottom wall of the partition 422 and the top opening 4212. This arrangement prevents microdroplets entering the first channel 42111 from falling into the inlet 4331 of the media outlet 433 after passing down the partition 422, thus avoiding waste due to accidental discharge outside the collection chamber 4211. For example, one end of the media outlet 433 extending outside the collection chamber 4211 is configured as a drain outlet 4332.

[0104] Exemplarily, the collecting chamber 4211 further includes a bottom outlet 4215 for discharging microdroplets. Those skilled in the art will understand that the method of use in this embodiment is as follows: the liquid entering the collecting chamber 4211 consists of microdroplets with a density difference and a continuous phase liquid. Therefore, after entering the collecting chamber 4211, the microdroplets sink to the bottom of the collecting chamber 4211, while the continuous phase liquid is located above the microdroplets. As the collecting chamber 4211 is gradually filled with microdroplets and the continuous phase liquid, when the surface of the continuous phase liquid exceeds the inlet 4331, it is guided by the medium drain pipe 433 to the collecting chamber 4211, that is, separated from the microdroplets. Once the microdroplets in the collecting chamber 4211 reach a certain volume, the microdroplets are discharged from the collecting chamber 4211 through the bottom outlet 4215.

[0105] As further exemplarily, the area of ​​the collection container 421 surrounding the bottom outlet 4215 is configured as a conical guide portion 4216; the inner diameter of the guide portion 4216 increases in the direction of movement from the bottom outlet 4215 toward the top opening 4212. Those skilled in the art will understand that the conical guide portion 4216 can both accelerate the discharge speed of microdroplets from the collection cavity 4211 from the bottom outlet 4215 and prevent microdroplets from forming residues on the bottom wall of the collection cavity 4211, thereby further avoiding waste of microdroplets and indirectly increasing the yield of microdroplets.

[0106] Figure 19 The diagram shown is a cross-sectional view of an embodiment of this disclosure, including a liquid collection tube. Figure 20 The diagram shown is a cross-sectional view of a liquid collecting tube from another perspective in an embodiment of this disclosure. Figure 19 and Figure 20 In the example, the collection unit 420 further includes a liquid collection tube 434 disposed within the top opening 4212. The partition 422 is vertically disposed within the liquid collection tube 434, and the first channel 42111 and the second channel 42112 are formed within the inner cavity of the liquid collection tube 434; the collection cavity 4211 further includes a bottom outlet 4215 for the discharge of microdroplets; the liquid collection tube 434 and the bottom outlet 4215 are coaxial.

[0107] For example, the distance between the liquid inlet 4331 and the top opening 4212 is smaller than the distance between the bottom wall of the partition 422 and the top opening 4212 and the distance between the bottom wall of the collection tube 434 and the top opening 4212.

[0108] The advantages of the above configuration are that the liquid collection tube 434 can guide the microdroplets to the area corresponding to the bottom outlet 4215, so that the microdroplets can be discharged from the bottom outlet 4215 later. It also reduces the area where the liquid inlet 4331 can communicate with the liquid surface, thereby further reducing the possibility of microdroplets being discharged from the liquid inlet 4331, thus avoiding waste of microdroplets and indirectly increasing the yield of microdroplets.

[0109] exist Figure 19In the example, the collection container 421 includes a detachably connected collection cylinder 4213 and a cover plate 4214; the space enclosed by the collection cylinder 4213 and the cover plate 4214 forms the collection cavity 4211; the top opening 4212 is formed in the cover plate 4214, and the bottom outlet 4215 is formed in the bottom wall of the collection cylinder 4213. Exemplarily, the detachable connection of the collection cylinder 4213 and the cover plate 4214 is implemented as a screw connection or a snap-fit ​​connection. It is understood that the detachable connection of the collection cylinder 4213 and the cover plate 4214 facilitates cleaning of the inside of the collection cavity 4211 and the media drain pipe 433 by an operator. In this embodiment, the collection cylinder 4213 and the cover plate 4214 are connected by bolts. In another embodiment, the collection cylinder 4213 and the cover plate 4214 are snap-fit ​​connected.

[0110] exist Figure 18 and Figure 19 In the example, the media conduit 433 has multiple bends. In other embodiments, the media conduit 433 has one bend, for example... Figure 21 The diagram shows a cross-sectional view of another embodiment of the media manifold shown in this disclosure. Figure 21 In the example, the media drain pipe 433 has a bend. The media drain pipe 433 includes a vertical pipe section 4333 and an inclined pipe section 4334 connected in series; the inlet 4331 is formed at the top of the vertical pipe section 4333, and the end of the inclined pipe section 4334 extends to the outside of the collection chamber 4211 and forms the outlet 4332. Those skilled in the art will understand that the fewer bends in the media drain pipe 433, the lower the resistance to the continuous phase liquid flowing inside it, and the better the effect of discharging the continuous phase liquid from the collection chamber 4211. The inclined pipe section 4334 further improves the effect of discharging the continuous phase liquid from the collection chamber 4211.

[0111] In another embodiment, the medium pipe 433 is implemented as including a vertical pipe section and an arc pipe section connected in series; the arc end of the arc pipe section faces upward.

[0112] In summary, this disclosure provides a microdroplet production device, including a continuous phase supply component, a dispersed phase component, a microdroplet cutting component, and a collection component. The dispersed phase supply component includes at least one injection pump, with the injection pump tubing connected to a dispersed phase container. The continuous phase supply component is also connected to the continuous phase container. The microdroplet cutting component includes at least one microfluidic chip; the at least one microfluidic chip is connected to the injection pump to obtain dispersed phase liquid, and is also connected to the continuous phase supply component to obtain continuous phase liquid. The collection component includes a manifold unit and a collection unit; the manifold unit is connected to the microfluidic chip, and the collection unit is connected to the manifold unit to collect microdroplets. Through the cooperation of the dispersed phase supply component, the continuous phase supply component, the microfluidic chip, and the collection component, this disclosure can continuously supply continuous phase liquid and dispersed phase liquid to the microfluidic chip, thereby improving the microdroplet production rate.

[0113] The above embodiments are merely illustrative of the principles and effects of this disclosure and are not intended to limit this disclosure. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of this disclosure. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in this disclosure should still be covered by the protection scope of this disclosure.

Claims

1. A microdroplet production device, characterized in that, include: A dispersion phase supply assembly includes at least one injection pump; the injection pump is connected to the dispersion phase container. Continuous phase liquid supply assembly, with pipelines connected to the continuous phase container; A microdroplet cutting assembly includes at least one microfluidic chip; the at least one microfluidic chip is connected to the injection pump to obtain a dispersed phase liquid, and is also connected to the continuous phase liquid supply assembly to obtain a continuous phase liquid; The microfluidic chip includes a continuous phase channel, a dispersed phase channel, and a microdroplet collection chamber. The continuous phase channel is for loading a continuous phase liquid and has a first inlet and a first outlet. The dispersed phase channel is located within the continuous phase channel and is for loading a dispersed phase liquid. It has a second inlet and a second outlet surrounded by the first outlet. The flow directions of the dispersed phase channel and the continuous phase channel form a certain angle, so that the dispersed phase liquid is cut into microdroplets at the second outlet by the continuous phase liquid at the first outlet. The microdroplet collection chamber is connected to the first outlet and the second outlet. The inner diameter of the microdroplet collection chamber is larger than the inner diameter of the second outlet; The current collection assembly includes a current collection unit and a current collection unit; the current collection unit is connected to the microfluidic chip via a conduit, and the current collection unit is connected to the current collection unit via a conduit to collect microdroplets.

2. The microdroplet production equipment according to claim 1, characterized in that, At least one of the continuous phase flow channel and the dispersed phase flow channel is provided with a variable diameter section whose inner diameter decreases along the flow direction.

3. The microdroplet production equipment according to claim 1, characterized in that, The injection pump includes an infusion chamber and a heating element surrounding the infusion chamber; a transition assembly is provided between the injection pump and the microfluidic chip; the transition assembly includes at least one adapter, a continuous phase tube, and a heat-insulating tube; the adapter has a heat-insulating cavity, and a first interface and a second interface communicating with the heat-insulating cavity; the first interface is connected to the heating element; one end of the heat-insulating tube is connected to the second interface, and the other end is connected to a heating medium container; The continuous phase tube is disposed inside the insulation tube, with one end connected to the microfluidic chip and the other end extending out of the insulation cavity after passing through the second interface and connected to the infusion chamber.

4. The microdroplet production equipment according to claim 1, characterized in that, The injection pump includes an infusion chamber and a heating element surrounding the infusion chamber; a transition assembly is provided between the injection pump and the microfluidic chip; the transition assembly includes a first adapter, a second adapter, a continuous phase tube, and a heat-insulating tube; the first adapter has a first heat-insulating cavity, and a first interface and a second interface connecting to the first heat-insulating cavity; the second adapter of the pair of adapters has a second heat-insulating cavity, and a third interface and a fourth interface connecting to the second heat-insulating cavity; the first interface tube is connected to the heating element; one end of the heat-insulating tube is connected to the second interface, and the other end is connected to the fourth interface; the third interface tube is connected to the heating medium container; The continuous phase tube is disposed inside the insulation tube, and one end extends out of the first insulation cavity after passing through the second interface and is connected to the infusion chamber. The other end extends out of the second insulation cavity after passing through the fourth interface and is connected to the microfluidic chip.

5. The microdroplet production equipment according to claim 1, characterized in that, It also includes a cleaning unit; the cleaning unit includes a cleaning tube and at least one cleaning port connected to the cleaning tube, the at least one cleaning port being connected to the at least one injection pump.

6. The microdroplet production equipment according to claim 1, characterized in that, It also includes a water purification unit; the water purification unit includes a water purification pipe and at least one water purification outlet connected to the water purification pipe, the at least one water purification outlet being connected to the at least one injection pump.

7. The microdroplet production equipment according to claim 1, characterized in that, The microfluidic chip is implemented in multiple ways; each of the multiple microfluidic chips is connected in tubing to the at least one injection pump.

8. The microdroplet production equipment according to claim 1, characterized in that, Both the injection pump and the microfluidic chip are implemented in multiple ways; each microfluidic chip is connected to one of the injection pumps via tubing.

9. The microdroplet production equipment according to claim 1, characterized in that, The number of injection pumps is no greater than the number of microfluidic chips.

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