Breeding method of egg-use type green-shell Puan disc river black-bone chicken commercial line

Abstract

The invention discloses an egg-use type green-shell Puan dish river black-bone chicken commercial line seed production method, which is characterized in that blood relationships of red-feather black-tail chickens and bamboo-town chickens are introduced on the basis of blood relationships of Puan dish river black-bone chickens, and a ZX * (PA * HH) combination is formed by adopting three-line matching; according to the invention, the commercial green-shell Pu'an dish river silky fowl for egg replacement retains the variety characteristics and the market weight of the five-black Pu'an dish river silky fowl of black feather, black skin, black shin, black crown and black meat, the production economic benefit is higher than that of a green-shell egg of a powder-shell egg, the egg yield and the egg weight are obviously improved, and the production performance is further improved. Meanwhile, the egg-use type green-shell Pu'an disc river black-bone chicken commercial line obtained by the method is ideal in appearance, medium in body type, high in egg yield and large in egg weight, green-shell eggs are produced, the listed day age and the listed weight are similar to those of purebred Pu'an disc river black-bone chickens, and the egg-laying performance, the egg weight and the egg shell color are all superior to those of pure Pu'an disc river black-bone chickens. The method is suitable for popularization and application in the technical field of poultry breeding, especially in the technical field of Puan disc river black-bone chicken breeding.

Description

Technical Field

[0001] The invention relates to the technical field of poultry breeding, and in particular to a method for producing a pair of egg-type green-shell Pu'an Panjiang black-bone chicken breeding lines. Background Art

[0003] Local chickens have the characteristics of excellent meat and egg quality, but their production performance is relatively low. Therefore, on the basis of retaining the appearance characteristics and market weight of local chicken breeds, increasing their egg production can effectively improve the economic benefits within the breeding cycle, and is one of the cost-reduction and efficiency-increasing measures to cope with the high cost of feed raw materials. In the context of consumption upgrading, eggshell color and egg weight, as the two core indicators that affect consumer choices, are becoming key factors in reconstructing the market value of eggs. Studies have shown that the unique eggshell color (such as the natural green color of green-shelled eggs) not only meets consumers' aesthetic needs for product differentiation, but also conveys the quality associations of "high nutrition" and "ecological breeding" through visual cognition, making the price of green-shelled eggs 2-4 times that of ordinary eggs. The egg weight index directly affects family purchasing decisions. Large-sized eggs above 50g have a demand premium of 30% in the baking, maternity and infant markets. The coordinated improvement of these two traits can not only create brand memory points through eggshell color to increase product added value, but also rely on egg weight classification to meet diversified consumption scenarios, forming a dual competitiveness of "sensory value + practical value", and opening up a differentiated value growth space for the laying hen industry.

[0004] Pu'an Panjiang Black-bone Chicken is named after the Beipanjiang River Basin in Pu'an County, Qianxinan Prefecture, Guizhou Province, where it is mainly produced. It is also known as Pu'an Niujiaoshan Black-bone Chicken and Pu'an Black Golden Chicken. After a long period of natural and artificial selection, it has gradually formed a special black-bone chicken variety that can be used for both meat and medicine and is resistant to roughage. It has the characteristics of black feathers, black shanks, black skin, black meat, black bones, strong resistance to adversity, and delicious meat and eggs. It has become the main variety promoted by the local ecological poultry industry. However, the current development and utilization of Pu'an Panjiang Black-bone Chicken is still very low, and there are problems such as slow growth rate, low egg production, and small egg weight, which seriously restrict the high-quality development of the industry. Therefore, it is very necessary to carry out germplasm innovation for the local resource Pu'an Panjiang Black-bone Chicken.

[0005] Zhuxiang Chicken is named after the city of Chishui in Zunyi, Guizhou Province, which is the "hometown of bamboo". It is a local breed that can be used for both meat and eggs. Because the locals prefer black-bone individuals, they are intentionally selected and retained, resulting in the appearance characteristics of black hair, skin, meat, bones, beaks, crowns, and toes. The egg-laying performance is high and some groups can produce green-shelled eggs. It is an extremely precious and highly plastic breeding material, but its development and utilization level is still low. The red-feathered black-tailed chicken comes from the commercial generation of Babcock B380 laying hens. It is of medium size, with red feathers and black tails, high egg production, brown eggs, and large egg weight. After hybridization with colored feather roosters, it usually retains the appearance characteristics of the father, which can be used to improve the shortcomings of local breeds such as low egg production and small egg weight.

[0006] Therefore, by utilizing the advantages of the above materials, further hybridization and improvement of the Pu'an Panjiang black chicken can be carried out, while retaining its appearance characteristics, its egg-laying performance can be improved, and the egg-laying green-shell Pu'an Panjiang black chicken that can meet the needs of the southwest and south China regions can be bred. Summary of the invention

[0007] The technical problem to be solved by the present invention is to provide a method for producing a breeding stock of a matching system of green-shell Pu'an Panjiang black-bone chickens for egg production, which has ideal appearance, medium body shape, high egg production, large egg weight, green-shell eggs and stable performance.

[0008] The technical solution adopted by the present invention to solve the technical problem is: the egg-type green-shell Pu'an Panjiang black-bone chicken matching system seed production method comprises the following steps:

[0009] 1) Paternal breeding:

[0010] 101) Select the existing Zhuxiang Chicken ZX line, and breed the roosters that meet the weight standards and appearance standards at 16 weeks and 43 weeks of age, and the hens that meet the weight standards and egg production standards at 43 weeks of age, and the appearance standards, and select and breed the great-grandfather ZX paternal father and the great-grandfather ZX paternal mother according to the weight standards and appearance standards at 16 and 43 weeks of age for the roosters and the weight standards and egg production standards and appearance standards at 16 and 43 weeks of age for the hens;

[0011] 102) Breeding the great-grandfather ZX paternal line father and the great-grandfather ZX paternal line mother, and selecting and breeding according to the weight standard and appearance standard of roosters at 16 weeks and 43 weeks of age, and the weight standard and egg production standard and appearance standard of hens at 16 weeks and 43 weeks of age, respectively, to obtain the grandparent ZX paternal line father and the grandparent ZX paternal line mother;

[0012] 103) The grandparent ZX paternal line male parent and the grandparent ZX paternal line female parent are mated and selected according to the weight standard and appearance standard of the rooster at 16 weeks and 43 weeks of age, the semen quality standard of the rooster at 30 weeks of age, and the positive homozygous genotype of green shell eggs to obtain the parent ZX paternal line male parent;

[0013] 2) Maternal breeding:

[0014] 201) Breed the existing purebred Pu'an Panjiang black-breasted chicken PA line roosters that meet the weight and appearance standards at 16 weeks and 43 weeks of age and the hens that meet the weight standards at 16 weeks and 43 weeks of age and the egg production standards at 43 weeks of age and the appearance standards, and select and breed according to the egg production standards and appearance standards of the hens at 43 weeks of age to obtain the great-grandfather generation PA maternal father and the great-grandfather generation PA maternal mother;

[0015] 202), breeding the great-grandfather PA maternal sire with the great-grandfather PA maternal sire and selecting according to the egg production standard and appearance standard of the hen at 43 weeks of age to obtain the grandparent PA maternal sire;

[0016] 203) Use the existing red-feathered black-tailed hens HH line that meet the appearance characteristics and egg production at 43 weeks of age and body weight at 16 and 43 weeks of age as the grandparent HH maternal line mothers;

[0017] 204), breeding the grandparent PA maternal father and the grandparent HH maternal father, and selecting the parental PH maternal father according to the egg production of the hens at 43 weeks of age, the weight standards at 16 weeks and 43 weeks of age, and the appearance standards;

[0018] 3) The parent generation ZX paternal line sire obtained by paternal selection and the parent generation PH maternal line sire obtained by maternal selection can be bred and hybridized to obtain the egg-laying type green-shell Pu'an Panjiang black-bone chicken.

[0019] Furthermore, in steps 101) to 103), the selection and breeding of the great-grandparent ZX paternal father, the great-grandparent ZX paternal mother, the grandparent ZX paternal father, the grandparent ZX paternal mother, and the parent ZX paternal father are all carried out by single cage rearing and artificial insemination for pure breeding.

[0020] Furthermore, in steps 201) to 204), the breeding of the great-grandparent PA maternal sire, the great-grandparent PA maternal sire, the grandparent PA maternal sire, the grandparent HH maternal sire, and the parent PH maternal sire are all carried out by single cage breeding and artificial insemination.

[0021] Furthermore, in steps 101) to 103), the weight standard of the 16-week-old Zhuxiang chicken rooster is 1.75 kg, the weight standard of the 43-week-old is 2.1 kg, the egg production standard of the 43-week-old is that the same family hen lays more than 80 eggs at 43 weeks of age, the appearance characteristics of the Zhuxiang chicken rooster are strong and solid, the feathers have a dark green metallic luster, the sickle feathers are well developed, the feathers, the skin, the legs, the crown and the meat are black, and the semen quality requirement of the Zhuxiang chicken rooster is that the single semen collection volume reaches 0.5m L or more, milky white, thick, pH around 7-8, density 3-5 billion / mL, vitality above 0.7, fertilization rate above 90%; the egg production standard of the Zhuxiang chicken hen at 43 weeks of age is an average egg production of more than 80 eggs with green eggshell color, and an average egg weight of 50g; the weight standard of the Zhuxiang chicken hen at 16 weeks of age is 1.3kg and 43 weeks of age is 1.8kg; the appearance characteristics of the Zhuxiang chicken are delicate body shape, symmetrical structure, black feathers, black skin, black legs, black crown, and black meat.

[0022] Furthermore, in step 201) and step 202), the weight standard of the Pu'an Panjiang black-bone chicken rooster at 16 weeks of age is 1.4 kg, the weight standard at 43 weeks of age is 2.5 kg, the egg production standard at 43 weeks of age is an average egg production of hens of the same family at 43 weeks of age of more than 65 eggs, the appearance standard of the Pu'an Panjiang black-bone chicken rooster is a strong physique, black feathers, black skin, black shanks, black crown, and black meat, and the semen quality requirement of the Pu'an Panjiang black-bone chicken rooster is a single semen collection volume of 0.5m L or more, milky white, thick, pH around 7-8, density 3-5 billion / mL, vitality above 0.7, fertilization rate above 90%; the weight standard of the Pu'an Panjiang black-bone chicken hen at 16 weeks of age is 1.2kg, the weight standard at 43 weeks of age is 1.9kg, the egg production standard at 43 weeks of age is more than 65 eggs, and the eggshell color is pink, with an average egg weight of 48g; the appearance standard of the Pu'an Panjiang black-bone chicken hen is a medium size, black feathers, black skin, black legs, black crown, and black meat.

[0023] Furthermore, in step 203), the appearance characteristics of the red-feathered black-tailed hen HH are a slender body, red feathers, black tail, yellow shanks, a red single comb and white skin, an average body weight of 1.3 kg at 16 weeks of age, an average body weight of 1.8 kg at 43 weeks of age, an egg production of more than 150 eggs at 43 weeks of age, an average egg weight of 62.5 g, and a brown eggshell color.

[0024] Furthermore, in step 204), the PH hen has black feathers and dark skin, a standard weight of 1.2 kg at 16 weeks of age, a standard weight of 1.8 kg at 43 weeks of age, an egg production of 110 eggs at 43 weeks of age, an average egg weight of 55 g, and a light brown eggshell color.

[0025] Furthermore, in step 3), the obtained egg-laying green-shell Pu'an Panjiang black-bone chicken has the appearance characteristics of "black feathers, black skin, black shanks, black crown, and black meat", and the egg-laying green-shell Pu'an Panjiang black-bone chicken hens obtained have reduced egg production after 43 weeks of age and can be marketed as high-quality old hens; the obtained egg-laying green-shell Pu'an Panjiang black-bone chicken roosters can be marketed as high-quality broilers at 20-22 weeks of age.

[0026] Furthermore, before mating, the breeder chickens in the matching system are purified from salmonella and chicken leukemia.

[0027] The beneficial effects of the present invention are as follows: the egg-laying green-shell Pu'an Panjiang black-bone chicken matching system seed production method introduces the red-feathered black-tailed chicken and Zhuxiang chicken bloodlines on the basis of the Pu'an Panjiang black-bone chicken bloodline, and adopts the matching combination ZX×(PA×HH), that is, the Pu'an Panjiang black-bone chicken rooster is hybridized with the red-feathered black-tailed chicken hen, so as to improve the egg production and egg weight of the offspring under good hybrid advantage; further, the Zhuxiang chicken rooster is adopted as the terminal paternal parent, and the obtained commercial generation inherits the green-shell egg gene of the terminal paternal parent, the hens have excellent egg-laying performance and produce green-shell eggs with higher economic benefits, and can be sold as high-quality old hens with local characteristics after the egg-laying is finished. The egg-laying green-shell Pu'an Panjiang black-bone chicken obtained by the present invention contains 25% of Pu'an Panjiang black-bone chicken bloodline, 50% of Zhuxiang chicken bloodline, and 25% of red-feathered black-tailed chicken. The commercial generation retains the "five black" appearance characteristics of black feathers, black skin, black shanks, black crowns, and black meat of the purebred Pu'an Panjiang black-bone chicken, and the adult chickens have ideal body shape and appearance, are completely free of diseases, and have excellent production performance. The present invention gives full play to the high production performance of the grandparent maternal red-feathered black-tailed chicken HH line with high egg production and large egg weight, so that the egg production and egg weight of the egg-laying green-shell Pu'an Panjiang black-bone chicken at the age of 43 weeks are significantly improved; the green-shell egg gene of the terminal paternal Zhuxiang chicken ZX line, as well as the production performance with high egg-laying performance, are inherited, so that the commercial generation hens have excellent egg-laying performance and produce green-shell eggs with higher economic value. In addition, the egg-laying green-shell Pu'an Panjiang black-bone chicken obtained by the present invention retains the "five black" appearance characteristics of "black feathers, black skin, black shanks, black crown, and black meat" and the marketing weight of the purebred Pu'an Panjiang black-bone chicken, which can meet consumers' preference for the "five black" chickens. Roosters can be marketed as high-quality broilers at 20-22 weeks of age, with an average weight of 2kg, which is similar to the marketing age and marketing weight of the purebred Pu'an Panjiang black-bone chicken, and has good meat performance. Furthermore, the egg-laying green-shell Pu'an Panjiang black-bone chicken hens obtained by the present invention produce green-shell eggs before 43 weeks of age, and the egg production and average egg weight are significantly greater than those of purebred Pu'an Panjiang black-bone chickens; the average egg production at 43 weeks of age is 102 eggs, which is 37 eggs more than that of purebred Pu'an Panjiang black-bone chickens, greatly improving the egg-laying performance; the average egg weight is 52g, which is 4g heavier than that of purebred Pu'an Panjiang black-bone chicken eggs, and the eggshell color is green, which has a higher economic value than pink-shell eggs, and has a significant improvement effect on egg weight, eggshell color and other egg qualities. Furthermore, hens with "five black" appearance characteristics can be listed as local high-quality old hens with local characteristics after 43 weeks of age, and the egg production decreases, further improving the residual value of eliminated laying hens. Therefore, the egg-laying green-shell Pu'an Panjiang black-bone chicken obtained by the present invention has an ideal body shape and appearance, is completely free of diseases, and has excellent production performance; the commercial generation of the egg-laying green-shell Pu'an Panjiang black-bone chicken matching series retains the "five black" appearance characteristics of "black feathers, black skin, black shanks, black crown, and black meat" and the marketing weight of the purebred Pu'an Panjiang black-bone chicken, has an ideal appearance, a medium body shape, a high egg production rate, a large egg weight, and produces green-shell eggs. The marketing age and marketing weight are similar to those of the purebred Pu'an Panjiang black-bone chicken, and the egg-laying performance, egg weight, and eggshell color are all better than those of the purebred Pu'an Panjiang black-bone chicken.The present invention adopts the individual selection and family selection methods, and can accurately select the Pu'an Panjiang black-bone chicken matching system for laying eggs with uniform appearance, high egg production, large egg weight, green-shell eggs and stable performance. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a schematic diagram of the method for producing the egg-type green-shell Pu'an Panjiang black-bone chicken matching system according to the present invention;

[0029] Figure 2 This is a characteristic diagram of the appearance of the egg-type green-shell Pu'an Panjiang black-bone chicken seed production according to the present invention. DETAILED DESCRIPTION

[0030] like Figure 1-2 As shown, the egg-type green-shell Pu'an Panjiang black-bone chicken matching system seed production method comprises the following steps:

[0031] 1) Paternal breeding:

[0032] 101) Select the existing Zhuxiang Chicken ZX line, and breed the roosters that meet the weight standards and appearance standards at 16 weeks and 43 weeks of age, and the hens that meet the weight standards and egg production standards at 43 weeks of age, and the appearance standards, and select and breed the great-grandfather ZX paternal father and the great-grandfather ZX paternal mother according to the weight standards and appearance standards at 16 and 43 weeks of age for the roosters and the weight standards and egg production standards and appearance standards at 16 and 43 weeks of age for the hens;

[0033] 102) Breeding the great-grandfather ZX paternal line father and the great-grandfather ZX paternal line mother, and selecting and breeding according to the weight standard and appearance standard of roosters at 16 weeks and 43 weeks of age, and the weight standard and egg production standard and appearance standard of hens at 16 weeks and 43 weeks of age, respectively, to obtain the grandparent ZX paternal line father and the grandparent ZX paternal line mother;

[0034] 103) The grandparent ZX paternal line male parent and the grandparent ZX paternal line female parent are mated and selected according to the weight standard and appearance standard of the rooster at 16 weeks and 43 weeks of age, the semen quality standard of the rooster at 30 weeks of age, and the positive homozygous genotype of green shell eggs to obtain the parent ZX paternal line male parent;

[0035] In steps 101) to 103), the weight standard of the 16-week-old Zhuxiang chicken rooster is 1.75 kg, the weight standard of the 43-week-old is 2.1 kg, the egg production standard of the 43-week-old is that the same family hen lays more than 80 eggs at 43 weeks of age, the appearance characteristics of the Zhuxiang chicken rooster are strong and solid, feathers with dark green metallic luster, well-developed sickle feathers, black feathers, black skin, black shanks, black crown, and black meat, and the semen quality requirement of the Zhuxiang chicken rooster is that the single semen collection volume is more than 0.5 mL , milky white, thick, pH of about 7 to 8, density of 3 to 5 billion / mL, vitality of more than 0.7, fertilization rate of more than 90%; the egg production standard of the Zhuxiang chicken hen at 43 weeks of age is an average egg production of more than 80 eggs and the eggshell color is green, the average egg weight is 50g, the weight standard of the Zhuxiang chicken hen at 16 weeks of age is 1.3kg, and the weight at 43 weeks of age is 1.8kg. The appearance characteristics of the Zhuxiang chicken are delicate body shape, symmetrical structure, black feathers, black skin, black legs, black crown, and black meat. The green-shell egg positive homozygous genotype rooster is obtained in the following manner: according to the characteristic that the green-shell egg trait is autosomal dominant inheritance, a group detection of Zhuxiang chickens is carried out by using molecular marker technology, and a green-shell egg positive homozygous genotype is selected to obtain a ZX line with a green-shell egg rate of 100%; wherein the molecular marker technology is implemented by a patented technology with application number 201510194180.6, application date 2015-04-22, and patent name "Primer composition for identifying the genotype of green-shell laying hens and its application";

[0036] 2) Maternal breeding:

[0037] 201), breeding a rooster of the existing purebred Pu'an Panjiang black-bone chicken PA line that meets the weight standards and appearance standards at 16 weeks and 43 weeks of age with a hen of the weight standards at 16 weeks and 43 weeks of age and the egg production standard and appearance standard at 43 weeks of age, and selecting and breeding according to the egg production standard and appearance standard of the hen at 43 weeks of age to obtain the great-grandfather generation PA maternal father and the great-grandfather generation PA maternal mother; the weight standard of the Pu'an Panjiang black-bone chicken rooster at 16 weeks of age is 1.4kg, the weight standard at 43 weeks of age is 2.5kg, the egg production standard at 43 weeks of age is that the average egg production of hens of the same family at 43 weeks of age is more than 65 eggs, and the Pu'an Panjiang black-bone chicken rooster The appearance standard is a strong physique, black feathers, black skin, black shanks, black crown, and black meat. The semen quality requirements of the Pu'an Panjiang black-bone chicken rooster are a single semen collection volume of more than 0.5mL, milky white, thick, pH of about 7-8, density of 3-5 billion / mL, vitality of more than 0.7, and fertilization rate of more than 90%; the weight standard of the Pu'an Panjiang black-bone chicken hen at 16 weeks of age is 1.2kg, the weight standard at 43 weeks of age is 1.9kg, the egg production standard at 43 weeks of age is more than 65 eggs, and the eggshell color is pink, with an average egg weight of 48g. The appearance standard of the Pu'an Panjiang black-bone chicken hen is a medium body, black feathers, black skin, black shanks, black crown, and black meat;

[0038] 202), the great-grandfather PA maternal sire is mated with the great-grandfather PA maternal dam and the grandparent PA maternal sire is selected according to the egg production standard and appearance standard of the hen at 43 weeks of age to obtain the grandparent PA maternal sire; the weight standard of the Pu'an Panjiang black-bone chicken rooster at 16 weeks of age is 1.4 kg, the weight standard at 43 weeks of age is 2.5 kg, the egg production standard at 43 weeks of age is the average egg production of hens of the same family at 43 weeks of age is more than 65 eggs, the appearance standard of the Pu'an Panjiang black-bone chicken rooster is a strong physique, black feathers, black skin, black legs, black crown, black meat, the Pu'an Panjiang black-bone chicken rooster is a strong physique, black feathers, black skin, black legs, black crown, black meat, the Pu'an Panjiang black-bone chicken rooster is a strong physique, black feathers, black skin, black legs, black crown, black meat, the Pu'an Panjiang black-bone chicken rooster is a strong physique, black feathers, black skin, black legs, black crown, black meat, the Pu'an Panjiang black-bone chicken rooster is a strong rooster ...-bone chicken rooster is a The quality requirements for semen of roosters are that the single semen collection volume is more than 0.5 mL, milky white, thick, pH is about 7-8, density is 3-5 billion / mL, vitality is more than 0.7, and fertilization rate is more than 90%; the weight standard of the Pu'an Panjiang black-bone chicken hen at 16 weeks of age is 1.2 kg, the weight standard at 43 weeks of age is 1.9 kg, the egg production standard at 43 weeks of age is more than 65 eggs, the egg shell color is pink, and the average egg weight is 48g; the appearance standard of the Pu'an Panjiang black-bone chicken hen is medium size, black feathers, black skin, black legs, black crown, and black meat;

[0039] 203) The existing red-feathered black-tailed hen HH line that meets the appearance characteristics and egg production at 43 weeks of age and the weight at 16 and 43 weeks of age is used as the ancestral HH maternal parent; the appearance characteristics of the red-feathered black-tailed hen HH are a delicate body, red feathers, black tail, yellow shanks, a red single comb and white skin, an average weight of 1.3 kg at 16 weeks of age, an average weight of 1.8 kg at 43 weeks of age, an egg production of more than 150 eggs at 43 weeks of age, an average egg weight of 62.5 g, and a brown eggshell color;

[0040] 204), breeding the grandparent PA maternal father and the grandparent HH maternal mother, and selecting the parental PH maternal father according to the egg production of the hen at 43 weeks of age, the weight standard at 16 weeks and 43 weeks of age, and the appearance standard; the PH hen has black feathers and black skin, the weight standard at 16 weeks of age is 1.2 kg, the weight standard at 43 weeks of age is 1.8 kg, the egg production at 43 weeks of age is 110 eggs, the average egg weight is 55 g, and the eggshell color is light brown;

[0041] 3) The parental generation ZX paternal line sire obtained by paternal selection and the parental generation PH maternal line maternal line obtained by maternal selection can be crossed to obtain the egg-laying type green-shell Pu'an Panjiang black chicken. The obtained egg-laying type green-shell Pu'an Panjiang black chicken has the appearance characteristics and market weight of purebred Pu'an Panjiang black chicken of "black feathers, black skin, black legs, black crown, and black meat". The hens produce green-shell eggs before 43 weeks of age, and the average egg production at 43 weeks of age is 102 eggs, with an average egg weight of 52g. After 43 weeks of age, the egg production decreases and can be marketed as high-quality old hens with an average weight of 1.8kg; roosters can be marketed as high-quality broilers at 20-22 weeks of age, with an average weight of 2kg.

[0042] In the above embodiment, in order to ensure the systematic breeding of egg-type green-shell Pu'an Panjiang black-breasted chickens, in steps 101) to 103), the selection and breeding of the great-grandfather ZX paternal father, the great-grandfather ZX paternal mother, the grandparent ZX paternal father, the grandparent ZX paternal mother, and the parent ZX paternal father are all carried out by single cage breeding and artificial insemination for pure breeding. Further, in steps 201) to 204), the selection and breeding of the great-grandfather PA maternal father, the great-grandfather PA maternal mother, the grandparent PA maternal father, the grandparent HH maternal mother, and the parent PH maternal mother are all carried out by single cage breeding and artificial insemination for hybridization.

[0043] Furthermore, in order to ensure the healthy production of egg-laying green-shell Pu'an Panjiang black-bone chickens, the breeding chickens in the matching system are purified from Salmonella and chicken leukemia before breeding.

[0044] The present invention statistically analyzes the overall appearance characteristics, growth performance, egg-laying performance and slaughter performance of the obtained egg-laying green-shell Pu'an Panjiang black-bone chicken. Its appearance characteristics are shown in Table 1, its growth performance is shown in Table 2, its egg-laying performance is shown in Table 3, and its slaughter performance is shown in Table 4:

[0045] Table 1 Appearance characteristics

[0046]

[0047]

[0048] Table 2 Growth performance

[0049] Age / W Rooster weight / g Hen / g 0 35.57±3.01 33.29±3.28 2 124.39±8.72 117.06±5.84 4 278.97±9.66 242.16±10.04 6 479.92±12.76 425.74±14.12 8 784.07±39.11 670.72±26.93 10 1079.10±62.13 877.96±51.41 12 1322.74±72.38 1085.26±68.43 14 1487.13±82.69 1180.08±71.86 16 1638.19±87.88 1297.23±80.81 18 1785.44±91.26 1462.35±83.68

[0050] Table 3 Egg laying performance

[0051]

[0052]

[0053] Table 4 Slaughter performance

[0054] index 150-day-old rooster 300-day-old hens Weight before slaughter / g 2007.28±81.39 1829.31±60.47 Slaughter rate / % 89.46±0.83 88.62±0.77 Half-eviscerated rate 82.61±1.07 81.75±1.28 Full Eviscerated Rate 77.28±1.17 70.86±1.26 Chest muscle rate / % 15.34±0.98 16.19±0.66 Leg muscle rate / % 25.25±1.09 20.37±0.83 Abdominal fat rate / % 1.97±0.87 5.72±0.54

[0055] As shown in Tables 1 to 4, the commercial generation of egg-laying green-shell Pu'an Panjiang black-bone chickens have a purple-black single comb, black feathers with a metallic luster, black legs, black skin, and black meat, retaining the "five black" appearance characteristics of purebred Pu'an Panjiang black-bone chickens, namely "black feathers, black skin, black legs, black combs, and black meat" and market weight, which can meet consumers' preference for "five black" chickens. Hens produce green-shell eggs before 43 weeks of age, and the average egg production at 43 weeks is 102 eggs, which is 37 more than that of purebred Pu'an Panjiang black-bone chickens, greatly improving the egg-laying performance; the average egg weight is 52g, which is 4g heavier than that of purebred Pu'an Panjiang black-bone chicken eggs, and the eggshell color is green, which has a higher economic value than pink-shell eggs, and has a significant improvement effect on egg quality such as egg weight and eggshell color; furthermore, hens with "five black" appearance characteristics can be listed as local high-quality old hens after 43 weeks of age, and the egg production decreases, further improving the residual value of eliminated laying hens. Roosters at 20-22 weeks of age can be marketed as high-quality broilers, with an average weight of 2kg, which is similar to the market weight and market age of purebred Pu'an Panjiang black-bone chickens, and have good meat performance, which meets the expected results. Therefore, the egg-laying green-shell Pu'an Panjiang black-bone chickens obtained by the present invention have ideal body shape and appearance, complete disease purification, and excellent production performance; the egg-laying green-shell Pu'an Panjiang black-bone chicken matching system commercial generation retains the "five black" appearance characteristics and market weight of the purebred Pu'an Panjiang black-bone chickens, namely "black feathers, black skin, black shanks, black crowns, and black meat", with ideal appearance, medium body shape, high egg production, large egg weight, and green-shelled eggs. The market age and market weight are similar to those of purebred Pu'an Panjiang black-bone chickens, and the egg-laying performance, egg weight, and eggshell color are all better than those of the purebred Pu'an Panjiang black-bone chickens. Therefore, the present invention can provide better reference information for the breeding of the egg-laying green-shell Pu'an Panjiang black-bone chicken matching system.

[0056] The above description shows and describes several preferred embodiments of the invention, but as mentioned above, it should be understood that the invention is not limited to the form disclosed herein, and should not be regarded as excluding other embodiments, but can be used in various other combinations, modifications and environments, and can be modified within the scope of the invention concept described herein through the above teachings or the technology or knowledge of the relevant field. Changes and modifications made by those skilled in the art should be within the scope of protection of the claims attached to the invention without departing from the spirit and scope of the invention.

Claims

1. A method for producing a pair of egg-laying green-shell Pu'an Panjiang black-bone chickens, comprising the following steps: 1) Paternal breeding: 101) Select the existing Zhuxiang Chicken ZX line, and breed the roosters that meet the weight standards and appearance standards at 16 weeks and 43 weeks of age, and the hens that meet the weight standards and egg production standards at 43 weeks of age, and the appearance standards, and select and breed the great-grandfather ZX paternal father and the great-grandfather ZX paternal mother according to the weight standards and appearance standards at 16 and 43 weeks of age for the roosters and the weight standards and egg production standards and appearance standards at 16 and 43 weeks of age for the hens; 102) Breeding the great-grandfather ZX paternal line father and the great-grandfather ZX paternal line mother, and selecting and breeding according to the weight standard and appearance standard of roosters at 16 weeks and 43 weeks of age, and the weight standard and egg production standard and appearance standard of hens at 16 weeks and 43 weeks of age, respectively, to obtain the grandparent ZX paternal line father and the grandparent ZX paternal line mother; 103) The grandparent ZX paternal line male parent and the grandparent ZX paternal line female parent are mated and selected according to the weight standard and appearance standard of the rooster at 16 weeks and 43 weeks of age, the semen quality standard of the rooster at 30 weeks of age, and the positive homozygous genotype of green shell eggs to obtain the parent ZX paternal line male parent; 2) Maternal breeding: 201) Breed the existing purebred Pu'an Panjiang black-breasted chicken PA line roosters that meet the weight and appearance standards at 16 weeks and 43 weeks of age and the hens that meet the weight standards at 16 weeks and 43 weeks of age and the egg production standards at 43 weeks of age and the appearance standards, and select and breed according to the egg production standards and appearance standards of the hens at 43 weeks of age to obtain the great-grandfather generation PA maternal father and the great-grandfather generation PA maternal mother; 202), breeding the great-grandfather PA maternal sire with the great-grandfather PA maternal sire and selecting according to the egg production standard and appearance standard of the hen at 43 weeks of age to obtain the grandparent PA maternal sire; 203) Use the existing red-feathered black-tailed hens HH line that meet the appearance characteristics and egg production at 43 weeks of age and body weight at 16 and 43 weeks of age as the grandparent HH maternal line mothers; 204), breeding the grandparent PA maternal father and the grandparent HH maternal father, and selecting the parental PH maternal father according to the egg production of the hens at 43 weeks of age, the weight standards at 16 weeks and 43 weeks of age, and the appearance standards; 3) The parent generation ZX paternal line sire obtained by paternal selection and the parent generation PH maternal line sire obtained by maternal selection can be bred and hybridized to obtain the egg-laying type green-shell Pu'an Panjiang black-bone chicken.

2. The egg-laying green-shell Pu'an Panjiang black-bone chicken matching seed production method as claimed in claim 1, characterized in that: In steps 101) to 103), the selection and breeding of the great-grandfather ZX paternal father, the great-grandfather ZX paternal mother, the grandparent ZX paternal father, the grandparent ZX paternal mother, and the parent ZX paternal father are all carried out by single cage breeding and artificial insemination.

3. The egg-laying green-shell Pu'an Panjiang black-bone chicken breeding method according to claim 1, characterized in that: In steps 201) to 204), the selection and breeding of the great-grandparent PA maternal sire, the great-grandparent PA maternal maternal sire, the grandparent PA maternal sire, the grandparent HH maternal maternal sire, and the parent PH maternal maternal sire are all carried out by single cage breeding and artificial insemination.

4. The egg-laying green-shell Pu'an Panjiang black-bone chicken breeding method according to claim 1, characterized in that: In steps 101) to 103), the weight standard of the 16-week-old Zhuxiang chicken rooster is 1.75 kg, the weight standard of the 43-week-old is 2.1 kg, the egg production standard of the 43-week-old is that the same family hen lays more than 80 eggs at 43 weeks of age, the appearance characteristics of the Zhuxiang chicken rooster are strong and solid, feathers with dark green metallic luster, well-developed sickle feathers, black feathers, black skin, black shanks, black crown, and black meat, and the semen quality requirement of the Zhuxiang chicken rooster is that the single semen collection volume is more than 0.5 mL , milky white, thick, pH of about 7 to 8, density of 3 to 5 billion / mL, vitality of more than 0.7, fertilization rate of more than 90%; the egg production standard of the Zhuxiang chicken hen at 43 weeks of age is an average egg production of more than 80 eggs and the eggshell color is green, the average egg weight is 50g, the weight standard of the Zhuxiang chicken hen at 16 weeks of age is 1.3kg, and the weight at 43 weeks of age is 1.8kg. The appearance characteristics of the Zhuxiang chicken are delicate body shape, symmetrical structure, black feathers, black skin, black legs, black crown, and black meat.

5. The egg-laying green-shell Pu'an Panjiang black-bone chicken matching seed production method as claimed in claim 1, characterized in that: In step 201) and step 202), the weight standard of the Pu'an Panjiang black-bone chicken rooster at 16 weeks of age is 1.4 kg, the weight standard at 43 weeks of age is 2.5 kg, the egg production standard at 43 weeks of age is an average egg production of hens of the same family at 43 weeks of age of more than 65 eggs, the appearance standard of the Pu'an Panjiang black-bone chicken rooster is a strong physique, black feathers, black skin, black shanks, black crown, and black meat, and the semen quality requirement of the Pu'an Panjiang black-bone chicken rooster is a single semen collection volume of more than 0.5 mL , milky white, thick, pH of about 7 to 8, density of 3 to 5 billion / mL, vitality above 0.7, fertilization rate above 90%; the weight standard of the Pu'an Panjiang black-bone chicken hen at 16 weeks of age is 1.2kg, the weight standard at 43 weeks of age is 1.9kg, the egg production standard at 43 weeks of age is more than 65 eggs and the eggshell color is pink, the average egg weight is 48g, and the appearance standard of the Pu'an Panjiang black-bone chicken hen is medium size, black feathers, black skin, black legs, black crown, and black meat.

6. The egg-laying green-shell Pu'an Panjiang black-bone chicken matching seed production method as claimed in claim 1, characterized in that: In step 203), the appearance characteristics of the red-feathered black-tailed hen HH are a slender body, red feathers, black tail, yellow shanks, a red single comb and white skin, an average body weight of 1.3 kg at 16 weeks of age, an average body weight of 1.8 kg at 43 weeks of age, an egg production of more than 150 eggs at 43 weeks of age, an average egg weight of 62.5 g, and a brown eggshell color.

7. The egg-laying green-shell Pu'an Panjiang black-bone chicken breeding method according to claim 1, characterized in that: In step 204), the PH hen has black feathers and dark skin, a standard weight of 1.2 kg at 16 weeks of age, a standard weight of 1.8 kg at 43 weeks of age, an egg production of 110 eggs at 43 weeks of age, an average egg weight of 55 g, and a light brown eggshell color.

8. The egg-laying green-shell Pu'an Panjiang black-bone chicken matching seed production method as claimed in claim 1, characterized in that: In step 3), the obtained egg-laying green-shell Pu'an Panjiang black-bone chicken has the appearance characteristics of "black feathers, black skin, black shanks, black crown, and black meat". The egg-laying green-shell Pu'an Panjiang black-bone chicken hens obtained can be marketed as high-quality old hens after the egg production decreases after 43 weeks of age; the egg-laying green-shell Pu'an Panjiang black-bone chicken roosters obtained can be marketed as high-quality broilers at 20-22 weeks of age.

9. The egg-laying green-shell Pu'an Panjiang black-bone chicken breeding method according to claim 1, characterized in that: Before mating, all breeder chickens in the matching system are purified from Salmonella and chicken leukemia.

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