Temperature control type cell culture box for cell therapy
By using a magnetic sealing mechanism and a semiconductor heat pump system in the cell incubator, absolute sealing and precise temperature control of the culture environment are achieved, and energy-saving energy is recovered through heat exchange, which solves the problems of instability and energy waste in the cell culture environment in the prior art, and an efficient and energy-saving cell culture environment is achieved.
Patent Information
- Application Number
- CN202510100274.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-22
- Publication Date
- 2025-05-02
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
When the existing cell culture incubator for cell therapy is regulating the temperature, the culture environment is not absolutely sealed, causing external air to enter and culture ambient air to be discharged, affecting the stability of the cell culture environment, and at the same time, the energy in the air with unsuitable temperature cannot be recovered, resulting in energy waste.
A temperature-controlled cell therapy cell incubator is designed, using a magnetic suction sealing mechanism and a semiconductor heat pump system to achieve absolute sealing of the culture environment. Through the combination of semiconductor heat pump and humidifier, precise control of temperature and humidity is achieved. At the same time, the heat exchange of air is used for heat insulated water tanks to recover unsuitable energy in the air.
It effectively avoids external air interfering with the carbon dioxide and oxygen content of the cell culture environment, ensures the stability of the cell culture environment, and at the same time, by recycling energy, energy waste is reduced and temperature control efficiency is improved.
Smart Images
Figure CN119913043A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of cell culture boxes, and in particular to a temperature-controlled cell culture box for cell therapy. Background Art
[0002] With the continuous advancement of cell therapy technology, such as the rapid development of stem cell therapy and immune cell therapy, the requirements for the cell culture environment are getting higher and higher. Cell therapy usually requires the removal of autologous cells, culture, proliferation and directed differentiation in vitro, and then re-infusion into the patient. In this process, the in vitro culture environment of cells is crucial, and temperature is one of the key factors affecting cell growth, metabolism and function. Therefore, a high-precision temperature-controlled cell culture incubator is needed to provide a stable and suitable culture environment for cell therapy. The existing cell culture incubators for cell therapy still have the following technical problems when in use, such as: 1. When using the existing cell culture incubator for cell therapy, the temperature of the culture environment is regulated. However, during the regulation, the cell culture environment is still in a relatively sealed state, which makes the outside air enter the culture environment faster due to the temperature control structure, and the air in the culture environment is also discharged to the outside faster, which can easily lead to a large change in the carbon dioxide content or oxygen content in the culture environment, which is not conducive to cell culture; 2. When the existing cell culture incubator for cell therapy is in use, during the temperature control process, air with inappropriate temperature, such as cold air when the temperature needs to be raised, or hot air when the temperature needs to be lowered, will be directly discharged to the outside, which will lead to energy waste and is not conducive to the recovery and reuse of cold energy in cold air and heat energy in hot air; Therefore, a temperature-controlled cell culture incubator for cell therapy is needed to solve the above problems. Summary of the invention
[0003] The purpose of the present invention is to provide a temperature-controlled cell culture incubator for cell therapy, so as to solve the problem in the above-mentioned background technology that the culture environment of the existing cell culture incubator for cell therapy is not absolutely sealed when adjusting the temperature, which easily affects the cell culture, and the energy in the air with unsuitable temperature cannot be recovered, resulting in energy waste.
[0004] To achieve the above object, the present invention provides the following technical solutions: A temperature-controlled cell culture box for cell therapy, comprising a box body, a support plate, a magnetic sealing mechanism, a temperature regulating mechanism and a humidity regulating mechanism, wherein a culture box with a door is arranged in the middle of the box body, and the interior of the culture box is slidably connected to the support plate, filter boxes are arranged on both sides of the box body, an air particle filter is arranged inside the filter box, and gas exchange holes penetrating the inside and outside of the filter box are evenly arranged on the filter box, a magnetic sealing mechanism is arranged on the partition between the filter box and the culture box, a temperature and humidity sensor is installed on the inner top end of the box body for monitoring the temperature and humidity conditions in the box body, and a control panel is arranged on the front side of the box body, a temperature regulating mechanism is arranged on the upper end of the box body, and the temperature regulating mechanism is connected to the humidity regulating mechanism, and the humidity regulating mechanism is installed on the rear side of the box body.
[0005] Preferably, the magnetic sealing mechanism includes an air permeable groove, a sealing plate and a spring, the air permeable groove is arranged through the filter bin and the culture bin, and the air permeable groove is arranged on the partition between the filter bin and the culture bin, the partition is provided with a recessed groove on the side facing the filter bin, and an electromagnet is installed at the bottom end of the recessed groove, limiting rods are evenly distributed on the top of the recessed groove, and the lower ends of the limiting rods are movably extended into the corresponding rod grooves one by one, the rod grooves are evenly distributed on the upper surface of the sealing plate, and an iron sheet is arranged on the lower surface of the sealing plate, a spring is arranged between the upper surface of the sealing plate and the top of the corresponding recessed groove, and the spring is movably nested in the outer side of the limiting rod.
[0006] Preferably, the temperature regulating mechanism includes air diffusion holes, a mounting frame and fan blades, the mounting frame is arranged on the box body, and a semiconductor heat pump is installed in the mounting frame, and the box body at the mounting frame position is provided with ventilation holes that penetrate the culture bin, both ends of the semiconductor heat pump are provided with air hoods, and the two air hoods are fixed to the upper surface of the mounting frame, each of the air hoods is provided with a support tube that penetrates the inner side thereof on the upper surface, and a servo motor is installed on the top of the support tube through a motor frame, the shaft end bearing of the servo motor penetrates into the interior of the support tube, and fan blades are provided on the outer side of the shaft end of the servo motor extending into the interior of the support tube, a Y-shaped tube is connected through the support tube, and the Y-shaped tube is connected through the gas collecting bin, the gas collecting bin is arranged inside the lower end of the box body, and the gas collecting bin is provided with air diffusion holes that penetrate to the culture bin.
[0007] Preferably, the semiconductor heat pump is electrically connected in series with the electromagnet, so as to start and stop the semiconductor heat pump and the electromagnet synchronously.
[0008] Preferably, the humidity regulating mechanism comprises an insulated water tank 1, an insulated water tank 2 and an exhaust hole, the insulated water tank 1 and the insulated water tank 2 are installed parallel to each other on the rear side of the box body, and the insulated water tank 1 and the insulated water tank 2 are provided with exhaust holes which are connected with the culture chamber above the liquid level, the lower ends of the insulated water tank 1 and the insulated water tank 2 are respectively connected with the corresponding liquid collecting chamber through two infusion tubes, and a humidifier is installed in the hole which is connected with the liquid collecting chamber and the culture chamber, and the insulated water tank 1 and the insulated water tank 2 are connected through two air ducts.
[0009] Preferably, the Y-shaped tube is provided with two air outlets, and the two parallel air outlets on the two Y-shaped tubes are both connected to the air collecting bin, and the two non-parallel air outlets extend below the liquid level in the insulated water tank one and the insulated water tank two respectively, and the upper ends of the insulated water tank one and the insulated water tank two are configured to be sealed.
[0010] Preferably, the Y-shaped pipe portion in the insulated water tank one and the insulated water tank two is spiral, so as to improve the efficiency and effect of heat exchange.
[0011] Preferably, the air guide tube is Z-shaped, and the two air guide tubes form an X-shaped structure, and the upper ends of the two air guide tubes are respectively connected to the liquid level above the first and second insulated water tanks.
[0012] Preferably, electromagnetic valves are provided on the lower end of the air guide tube, the Y-shaped tube and the exhaust hole to control the gas flow direction.
[0013] Preferably, the humidifier, semiconductor heat pump and servo motor are all electrically connected to the control panel, and the control panel is also electrically connected to the electromagnetic valves provided on the air duct, Y-shaped tube and exhaust hole, so that the processor in the control panel can control the start and stop of the humidifier, semiconductor heat pump and servo motor according to the data transmitted by the temperature and humidity sensor, and can also control the opening and closing of the electromagnetic valve.
[0014] Compared with the prior art, the beneficial effects of the present invention are: the temperature-controlled cell therapy cell culture box can prevent the outside air from being sucked into the cell culture environment during the temperature control process, thereby avoiding breaking the state of natural air exchange, so as to avoid causing a large degree of change in the carbon dioxide or oxygen content in the cell culture environment, thereby affecting cell culture, and can also recycle energy, avoiding the waste of energy directly discharged to the outside during the temperature control process: 1. When the temperature in the culture bin exceeds the set threshold, the semiconductor heat pump and the servo motor start, so that the two ends of the thermocouple on the semiconductor heat pump absorb heat and release heat respectively. At the same time, since the semiconductor heat pump is electrically connected in series with the electromagnet, the electromagnet will generate a strong suction force to adsorb the sealing plate, so that the sealing plate seals the air permeable groove. At this time, the culture bin will be in an absolutely sealed state. In addition, when the servo motor drives the fan blades to rotate, the air in the culture bin passes through the ventilation holes, the mounting frame and the support tube in turn. When the air in the culture bin passes through the mounting frame, it will exchange heat with the thermocouple end of the semiconductor heat pump, so that the air entering the two support tubes is hot air and cold air respectively. The electromagnetic valve on the Y-shaped tube is controlled to allow air of appropriate temperature to enter the air collecting bin and then return to the culture bin through the air diffusion holes, thereby realizing the regulation of the temperature in the culture bin; 2. The air with inappropriate temperature will enter the insulation water tank one or the insulation water tank two through the Y-shaped tube, and then perform heat exchange with the insulation water tank one or the insulation water tank two. The air after heat exchange will enter the insulation water tank one or the insulation water tank two above the liquid level by forming bubbles. In this process, the electromagnetic valve on the corresponding air guide pipe is opened to allow the air after heat exchange to enter the insulation water tank two or the insulation water tank one for heat exchange again. The temperature of the air after this heat exchange will be suitable for entering the culture chamber, so that it can enter the culture chamber through the exhaust hole to control the temperature. In addition, the air with appropriate or inappropriate temperature after passing through the semiconductor heat pump is returned to the culture chamber to form an internal circulation, so as to avoid the outside air interfering with the carbon dioxide or oxygen content in the cell culture environment, which is helpful for cell culture. By performing secondary heat exchange on the air with inappropriate temperature, energy can be saved and energy can be avoided from being directly discharged to the outside to cause waste. 3. By exchanging heat with air of inappropriate temperature, the water temperature in the insulation water tank 1 and the insulation water tank 2 will be higher and lower respectively. Then, when the humidity is low and the temperature is high or the humidity is low and the temperature is low in the culture chamber, there is no need to start the semiconductor heat pump. It is only necessary to start the humidifier at the corresponding position to achieve humidification and simultaneous heating or cooling, which helps to utilize recycled energy. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] Figure 1 It is a schematic diagram of the main structure of the present invention; Figure 2 It is a rear view structural schematic diagram of the present invention; Figure 3 This is a schematic diagram of the connection structure between the Y-shaped tube and the gas collecting bin of the present invention; Figure 4 For the present invention Figure 3 A schematic diagram of the enlarged structure of point A; Figure 5 It is a schematic diagram of a partial cross-sectional structure of the present invention; Figure 6 It is a schematic diagram of the longitudinal structure of the present invention; Figure 7 For the present invention Figure 6 Schematic diagram of the enlarged structure of point B.
[0016] In the figure: 1. box body; 2. support plate; 3. gas exchange hole; 4. air diffusion hole; 5. insulation water tank one; 6. insulation water tank two; 7. air guide tube; 8. Y-shaped tube; 9. humidifier; 10. gas collecting bin; 11. installation frame; 12. semiconductor heat pump; 13. wind hood; 14. support tube; 15. servo motor; 16. motor frame; 17. fan blade; 18. liquid collecting bin; 19. infusion tube; 20. filter bin; 21. exhaust hole; 22. ventilation groove; 23. electromagnet; 24. sealing plate; 25. rod groove; 26. limit rod; 27. spring; 28. culture bin. DETAILED DESCRIPTION
[0017] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0018] See also Figure 1-Figure 7 , the present invention provides the following technical solutions: Embodiment 1: In order to solve the problem that the culture environment of a cell culture box for cell therapy is not absolutely sealed when adjusting the temperature, which is not conducive to cell culture, the following technical solution is provided, specifically, a temperature-controlled cell culture box for cell therapy, including a box body 1, a support plate 2, a magnetic sealing mechanism, a temperature regulating mechanism and a humidity regulating mechanism. A culture box 28 with a door is provided in the middle of the box body 1, and the support plate 2 is slidably connected to the inside of the culture box 28. Filter boxes 20 are provided on both sides of the box body 1. An air particle filter is provided inside the filter box 20, and gas exchange holes 3 that pass through the inside and outside of the filter box 20 are evenly provided on the filter box 20. A magnetic sealing mechanism is provided on the partition between the filter box 20 and the culture box 28. A temperature and humidity sensor is installed on the inner top of the box body 1 for monitoring the temperature and humidity conditions in the box body 1, and a control panel is provided on the front side of the box body 1. A temperature regulating mechanism is provided on the upper end of the box body 1, and the temperature regulating mechanism is connected to the humidity regulating mechanism.
[0019] The magnetic sealing mechanism includes a breathable groove 22, a sealing plate 24 and a spring 27. The breathable groove 22 runs through the filter bin 20 and the culture bin 28, and the breathable groove 22 is arranged on the partition between the filter bin 20 and the culture bin 28. A recessed groove is arranged on the side of the partition facing the filter bin 20, and an electromagnet 23 is installed at the bottom end of the recessed groove. Limiting rods 26 are evenly distributed on the top of the recessed groove, and the lower ends of the limiting rods 26 are movably extended into the corresponding rod grooves 25 one by one. The rod grooves 25 are evenly distributed on the upper surface of the sealing plate 24, and an iron sheet is arranged on the lower surface of the sealing plate 24. A spring 27 is arranged between the upper surface of the sealing plate 24 and the top of the corresponding recessed groove, and The spring 27 is movably nested on the outside of the limit rod 26. When in use, the temperature threshold is set through the control panel. The temperature and humidity sensor monitors the temperature and humidity in the culture chamber 28 and feeds back to the control panel. When the temperature in the culture chamber 28 exceeds the threshold, the control panel will start the semiconductor heat pump 12 and energize the electromagnet 23 at the same time. After the electromagnet 23 is energized, it will have a strong magnetic force, which will attract the iron sheet set on the lower surface of the sealing plate 24, causing the sealing plate 24 to move downward until the iron sheet on the sealing plate 24 is adsorbed on the electromagnet 23. At this time, the sealing plate 24 will completely block the air permeable groove 22, so that the culture chamber 28 is in an absolutely sealed state.
[0020] The temperature regulating mechanism includes an air diffusion hole 4, a mounting frame 11 and a fan blade 17. The mounting frame 11 is arranged on the housing 1, and a semiconductor heat pump 12 is installed in the mounting frame 11, and a ventilation hole that penetrates the culture bin 28 is arranged on the housing 1 at the position of the mounting frame 11. Both ends of the semiconductor heat pump 12 are provided with a hood 13, and the two hoods 13 are fixed to the upper surface of the mounting frame 11. The upper surface of each hood 13 is provided with a support tube 14 that penetrates the inner side thereof, and a servo motor 15 is installed at the top of the support tube 14 through a motor frame 16. The shaft end bearing of the servo motor 15 penetrates the interior of the support tube 14, and the outer side of the shaft end of the servo motor 15 extending into the interior of the support tube 14 is provided with a fan blade 17. The support tube 14 is connected with a Y-shaped tube 8, and the Y-shaped tube 8 is connected with the gas collecting bin 10, and the gas collecting bin 10 is arranged on the housing 1 Inside the lower end, the gas collecting bin 10 is provided with an air diffusion hole 4 which passes through the culture bin 28. When in use, the semiconductor heat pump 12 is energized, causing the two ends of the electric couple thereon to absorb and release heat respectively. When the two ends of the electric couple on the semiconductor heat pump 12 absorb and release heat, the servo motor 15 starts and drives the fan blades 17, which will make the air in the absolutely sealed culture bin 28 pass through the mounting frame 11, the semiconductor heat pump 12, the wind hood 13 and the support tube 14 in sequence. In the process of the air passing through the semiconductor heat pump 12, the first heat exchange will occur, and then the hot air and the cold air will enter the two support tubes 14 respectively. The air entering the support tube 14 will be opened and closed by the electromagnetic valve on the Y-shaped tube 8, so that the air with a suitable temperature enters the gas collecting bin 10, and then returns to the culture bin 28 through the air diffusion hole 4, thereby regulating the temperature in the culture bin 28.
[0021] The semiconductor heat pump 12 and the electromagnet 23 are electrically connected in series, so as to start and stop the semiconductor heat pump 12 and the electromagnet 23 synchronously.
[0022] Embodiment 2: To solve the problem that in conventional cell culture incubators for cell therapy, air with inappropriate temperature is directly discharged to the outside when adjusting the temperature, resulting in energy waste, the following technical solution is provided, specifically, the humidity adjustment mechanism is installed on the rear side of the box body 1.
[0023] The humidity regulating mechanism comprises an insulated water tank 1 5, an insulated water tank 2 6 and an exhaust hole 21. The insulated water tank 1 5 and the insulated water tank 2 6 are installed parallel to each other on the rear side of the box body 1, and the insulated water tank 1 5 and the insulated water tank 2 6 are provided with an exhaust hole 21 which is connected to the culture bin 28 above the liquid level inside. The lower ends of the insulated water tank 1 5 and the insulated water tank 2 6 are connected to the corresponding liquid collection bin 18 through two infusion pipes 19, and a humidifier 9 is installed in the hole through which the liquid collection bin 18 and the culture bin 28 are connected. The insulated water tank 1 5 and the insulated water tank 2 6 are connected through two air guide pipes 7. The Y-shaped tube 8 is connected to the heat preservation tank 1 5 and the heat preservation tank 2 6. Two air outlets are arranged on the Y-shaped tube 8, and the two air outlets parallel to each other on the two Y-shaped tubes 8 are connected to the air collecting bin 10, and the two non-parallel air outlets are respectively extended to below the liquid level in the heat preservation water tank 1 5 and the heat preservation water tank 2 6. The upper ends of the heat preservation water tank 1 5 and the heat preservation water tank 2 6 are arranged to be sealed. When in use, air with inappropriate temperature enters the heat preservation water tank 1 5 or the heat preservation water tank 2 6 through the Y-shaped tube 8, and a second heat exchange is performed, so that the cold energy or heat energy carried in the air is recovered, and the air with recovered cold energy or heat energy enters the heat preservation water tank 1 through the air guide pipe 7. The third heat exchange is performed in the insulated water tank 1 6 or the insulated water tank 1 5, so that the air temperature at this time is suitable for regulating the temperature in the culture chamber 28, and then returns to the culture chamber 28 through the exhaust hole 21 to form an internal circulation, which not only realizes the regulation of the temperature in the culture chamber 28, but also can recycle and reuse the cold energy or heat energy. The Y-shaped pipe 8 in the insulated water tank 1 5 and the insulated water tank 2 6 is spiral-shaped to improve the efficiency and effect of heat exchange, the air guide pipe 7 is Z-shaped, and the two air guide pipes 7 form an X-shaped structure, and the upper ends of the two air guide pipes 7 are respectively connected to the insulated water tank 1 5 and the insulated water tank 2 6. The air duct 7 and the Y-shaped tube 8 are connected above the liquid level of the heat preservation water tank 1 5 and the second heat preservation water tank 6. Electromagnetic valves are provided on the lower end of the air duct 7, the Y-shaped tube 8 and the exhaust hole 21 to control the flow direction of the gas. The humidifier 9, the semiconductor heat pump 12 and the servo motor 15 are electrically connected to the control panel, and the control panel is also electrically connected to the electromagnetic valves provided on the air duct 7, the Y-shaped tube 8 and the exhaust hole 21, so that the processor in the control panel can control the start and stop of the humidifier 9, the semiconductor heat pump 12 and the servo motor 15 according to the data transmitted by the temperature and humidity sensor, and can also control the opening and closing of the electromagnetic valve.
[0024] The contents not described in detail in this specification belong to the prior art known to professional and technical personnel in this field.
[0025] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A temperature-controlled cell culture box for cell therapy, comprising a box body (1), a support plate (2), a magnetic sealing mechanism, a temperature regulating mechanism and a humidity regulating mechanism, characterized in that: A culture chamber (28) with a chamber door is arranged in the middle of the box (1), and a support plate (2) is slidably connected to the interior of the culture chamber (28). Filter chambers (20) are arranged on both sides of the box (1), and an air particle filter is arranged inside the filter chamber (20). The filter chamber (20) is evenly provided with gas exchange holes (3) that penetrate the inside and outside of the filter chamber (20). A magnetic sealing mechanism is arranged on the partition between the filter chamber (20) and the culture chamber (28). A temperature and humidity sensor is installed at the inner top of the box (1) for monitoring the temperature and humidity conditions in the box (1), and a control panel is arranged on the front side of the box (1). A temperature adjustment mechanism is arranged at the upper end of the box (1), and the temperature adjustment mechanism is connected to the humidity adjustment mechanism. The humidity adjustment mechanism is installed on the rear side of the box (1).
2. A temperature-controlled cell culture incubator for cell therapy according to claim 1, characterized in that: The magnetic sealing mechanism comprises a venting groove (22), a sealing plate (24) and a spring (27); the venting groove (22) is arranged to pass through the filter chamber (20) and the culture chamber (28); the venting groove (22) is arranged on a partition between the filter chamber (20) and the culture chamber (28); a recessed groove is arranged on a side of the partition facing the filter chamber (20); an electromagnet (23) is installed at the bottom end of the recessed groove; limiting rods (26) are evenly distributed at the top end of the recessed groove; the lower ends of the limiting rods (26) are movably extended into corresponding rod grooves (25); the rod grooves (25) are evenly distributed on the upper surface of the sealing plate (24); an iron sheet is arranged on the lower surface of the sealing plate (24); a spring (27) is arranged between the upper surface of the sealing plate (24) and the top end of the corresponding recessed groove; and the spring (27) is movably nested on the outer side of the limiting rod (26).
3. A temperature-controlled cell culture incubator for cell therapy according to claim 2, characterized in that: The temperature regulating mechanism comprises an air diffusion hole (4), a mounting frame (11) and a fan blade (17); the mounting frame (11) is arranged on the housing (1), and a semiconductor heat pump (12) is installed in the mounting frame (11); a ventilation hole which is connected to the culture chamber (28) is arranged on the housing (1) at the location of the mounting frame (11); wind shields (13) are arranged at both ends of the semiconductor heat pump (12), and the two wind shields (13) are fixed to the upper surface of the mounting frame (11); each wind shield (13) is provided with a support pipe (14) which passes through the inner side thereof on the upper surface; A servo motor (15) is installed at the top end of the support tube (14) through a motor frame (16); the shaft end bearing of the servo motor (15) penetrates into the interior of the support tube (14); and a fan blade (17) is arranged on the outer side of the shaft end of the servo motor (15) extending into the interior of the support tube (14); a Y-shaped tube (8) is connected through the support tube (14); and the Y-shaped tube (8) is connected through the gas collecting bin (10); the gas collecting bin (10) is arranged inside the lower end of the box body (1), and the gas collecting bin (10) is provided with a gas diffusion hole (4) that penetrates to the culture bin (28).
4. A temperature-controlled cell culture incubator for cell therapy according to claim 3, characterized in that: The semiconductor heat pump (12) and the electromagnet (23) are electrically connected in series, and are used for synchronously starting and stopping the semiconductor heat pump (12) and the electromagnet (23).
5. A temperature-controlled cell culture incubator for cell therapy according to claim 4, characterized in that: The humidity regulating mechanism comprises an insulation water tank 1 (5), an insulation water tank 2 (6) and an exhaust hole (21); the insulation water tank 1 (5) and the insulation water tank 2 (6) are installed parallel to each other on the rear side of the box body (1); and the insulation water tank 1 (5) and the insulation water tank 2 (6) are both provided with an exhaust hole (21) above the liquid level inside the insulation water tank 1 (5) and the insulation water tank 2 (6) and communicating with the culture chamber (28); the lower ends of the insulation water tank 1 (5) and the insulation water tank 2 (6) are respectively connected to the corresponding liquid collecting chamber (18) through two infusion tubes (19); and a humidifier (9) is installed in the hole communicating between the liquid collecting chamber (18) and the culture chamber (28); and the insulation water tank 1 (5) and the insulation water tank 2 (6) are connected through two air guide tubes (7).
6. A temperature-controlled cell culture incubator for cell therapy according to claim 5, characterized in that: The Y-shaped tube (8) is provided with two air outlets, and the two air outlets on the two Y-shaped tubes (8) are parallel to each other and are connected to the air collecting bin (10), and the two non-parallel air outlets extend below the liquid level in the first insulation water tank (5) and the second insulation water tank (6), respectively. The upper ends of the first insulation water tank (5) and the second insulation water tank (6) are configured to be sealed.
7. A temperature-controlled cell culture incubator for cell therapy according to claim 6, characterized in that: The Y-shaped tube (8) in the first insulation water tank (5) and the second insulation water tank (6) is partially spiral-shaped, so as to improve the efficiency and effect of heat exchange.
8. A temperature-controlled cell culture incubator for cell therapy according to claim 7, characterized in that: The air guide pipe (7) is Z-shaped, and the two air guide pipes (7) form an X-shaped structure, and the upper ends of the two air guide pipes (7) are respectively connected to the liquid level of the first insulation water tank (5) and the second insulation water tank (6).
9. A temperature-controlled cell culture incubator for cell therapy according to claim 8, characterized in that: The lower end of the air guide tube (7), the Y-shaped tube (8) and the exhaust hole (21) are all provided with electromagnetic valves for controlling the flow direction of the gas.
10. A temperature-controlled cell culture incubator for cell therapy according to claim 9, characterized in that: The humidifier (9), semiconductor heat pump (12) and servo motor (15) are all electrically connected to the control panel, and the control panel is also electrically connected to the air duct (7), the Y-shaped tube (8) and the electromagnetic valve provided on the exhaust hole (21), so that the processor in the control panel can control the start and stop of the humidifier (9), semiconductor heat pump (12) and servo motor (15) according to the data transmitted by the temperature and humidity sensor, and can also control the opening and closing of the electromagnetic valve.