Pre-packaged micro-fluidic chip and cell detection device

Abstract

The invention relates to a pre-packaged micro-fluidic chip and a cell detection device. The pre-packaged micro-fluidic chip comprises a chip main body which comprises a supporting column and a photoelectric detection channel, and the photoelectric detection channel is provided with an exhaust port; the circulation chamber is arranged on the chip main body and is communicated with the photoelectric detection channel, the circulation chamber comprises a storage chamber and a mixing chamber, detection freeze-dried powder is stored in the storage chamber, the storage chamber comprises a sample adding port, the sample adding port is used for adding a to-be-detected sample, a barrier column is arranged in the mixing chamber, and the barrier column is connected with the storage chamber. The mixing chamber is communicated with the photoelectric detection channel, and the storage chamber abuts against the supporting column. According to the invention, the to-be-detected sample and the dissolved freeze-dried powder can be fully mixed to improve the cracking effect, and the to-be-detected sample can be rapidly detected.

Description

Technical Field

[0001] The present application relates to the field of cell detection technology, and in particular to a prepackaged microfluidic chip and a cell detection device. Background Art

[0002] With the continuous advancement of science and technology, the application of cell detection in various industries has become more and more extensive, especially in food safety, medical diagnosis and scientific research, the importance of cell detection has become increasingly prominent. Somatic cell and bacterial detection in the food industry, body fluid cell detection in the medical field, and cell detection of culture fluid in scientific research experiments have become key links. These tests often rely on highly sensitive and specific technical means, such as flow cytometry, fluorescence microscopy and PCR technology, to ensure the accuracy and reliability of the test results. Effective cell detection can help quickly assess health status, diagnose diseases, ensure food quality, and promote the progress of scientific research.

[0003] Among the related technologies, commonly used cell detection methods include flow cytometry, microscope counting, and PCR detection. Flow cytometry is one of the most widely used technologies for quantitative cell analysis. It uses laser beams to stimulate markers on cells to achieve high-precision cell counting and classification. The microscope counting method calculates the number of cells by manually observing the sample. It is simple to operate but has high accuracy and is suitable for small-scale cell detection. PCR technology indirectly reflects the number and type of cells by amplifying specific genes.

[0004] However, in related technologies, flow cytometry equipment is expensive and requires professional operators, and the reagents are often toxic, which limits the application of rapid response. Although microscope counting is accurate, it relies on manual operation, is inefficient, and has a long detection cycle when the sample size is large. Although PCR technology is sensitive, it is complex to operate, time-consuming, and the equipment is expensive, making it difficult to meet the needs of rapid on-site detection. Summary of the invention

[0005] Based on this, it is necessary to provide a pre-packaged microfluidic chip and a cell detection device that can quickly detect and accurately measure the number of somatic cells based on the bioluminescence intensity to address the above technical problems.

[0006] In a first aspect, the present application provides a prepackaged microfluidic chip. The prepackaged microfluidic chip comprises:

[0007] The chip body includes a support column and a photoelectric detection channel, and the photoelectric detection channel is provided with an exhaust port;

[0008] A circulation chamber is arranged on the chip body and is connected with the photoelectric detection channel. The circulation chamber includes a storage chamber and a mixing chamber. The storage chamber stores freeze-dried powder for detection, and the storage chamber includes a sample addition port, which is used to add samples to be detected. An obstacle column is arranged in the mixing chamber, which is connected with the photoelectric detection channel, and the storage chamber abuts the support column.

[0009] In one embodiment, the mixing chamber includes a mixing chamber and a circulation channel, the mixing chamber is communicated with the storage chamber, and the mixing chamber is connected to the photoelectric detection channel through the circulation channel, and the volume of the storage chamber is equal to the volume of the photoelectric detection channel;

[0010] The obstacle column comprises a first obstacle column, which is arranged in the mixing chamber and has an olive-shaped cross section.

[0011] In one embodiment, the obstacle column further includes a second obstacle column, which is disposed in the flow channel, has a cross section of a water drop shape, and has a pointed end facing the mixing chamber.

[0012] In one embodiment, the prepackaged microfluidic chip further includes a counterweight, which is disposed on the chip body and on the other side of the support column relative to the flow chamber.

[0013] In a second aspect, the present application provides a cell detection device, comprising the prepackaged microfluidic chip described in any of the above embodiments. The cell detection device also includes:

[0014] Device housing;

[0015] A control assembly, the control assembly being arranged on the inner wall of the device housing;

[0016] A carrier module, the carrier module is placed at the inner bottom of the device housing, and the pre-packaged microfluidic chip is placed on the carrier module;

[0017] The photoelectric detection module is arranged on the inner side of the device housing.

[0018] In one embodiment, the device housing comprises:

[0019] The outer shell comprises a viewing window, an assembly window and a sliding cover plate, wherein the viewing window and the assembly window are both arranged on the front-view shell plate of the outer shell, and the sliding cover plate is embedded in the first side plate of the outer shell;

[0020] The shading plate is arranged inside the outer shell, the shading plate is slidably connected with the inner rear plate and the right side plate of the outer shell respectively, and the photoelectric detection module is arranged on the shading plate.

[0021] In one embodiment, the front-view shell plate includes a lower shell plate and an upper shell plate, the lower shell plate is perpendicular to the bottom surface of the outer shell, and the upper shell plate is arranged at an angle with the lower shell plate;

[0022] The viewing window is arranged on the upper shell plate, and the assembly window is arranged on the lower shell plate.

[0023] In one embodiment, the control component includes:

[0024] A main control board, which is arranged on the inner side of the second side board of the outer shell;

[0025] A touch screen, which is embedded in the assembly window and connected to the main control board;

[0026] The power supply unit is arranged below the main control board and is connected to the main control board.

[0027] In one embodiment, the carrier module comprises:

[0028] A linear transport assembly, the linear transport assembly is arranged at the inner bottom of the outer shell, the linear transport assembly comprises a fixed base, a first fixed vertical plate, a second fixed vertical plate, a guide rod, a lead screw, a first motor and a loading plate, the fixed base is arranged at the inner bottom of the outer shell, the first fixed vertical plate and the second fixed vertical plate are arranged at two ends of the fixed base respectively, a guide rod is connected between the first fixed vertical plate and the second fixed vertical plate, a first end of the lead screw passes through the first fixed vertical plate, and a second end of the lead screw is connected to the second fixed vertical plate, a first motor is arranged on the outer side of the first fixed vertical plate, and the first motor is connected to the first end of the lead screw through a coupling, and both the guide rod and the lead screw pass through the loading plate;

[0029] The rotating assembly is arranged on the carrier plate, and the rotating assembly comprises a second motor, the second motor is fixedly connected to the carrier plate, and the pre-packaged microfluidic chip is connected to the rotating shaft of the second motor.

[0030] In one embodiment, the photoelectric detection module includes:

[0031] The photomultiplier tube module is arranged on the light shielding plate, and the photomultiplier tube module comprises a photosensitive area, and the photosensitive area faces the pre-packaged microfluidic chip.

[0032] The above-mentioned prepackaged microfluidic chip and cell detection device add the sample to be detected, that is, the liquid to be detected, from the sample addition port of the storage chamber in the flow chamber. After the liquid to be detected enters the storage chamber, the stored lyophilized powder is dissolved to obtain a mixed liquid to be detected. By operating the prepackaged microfluidic chip, the mixed liquid to be detected flows through the mixing chamber to enter the photoelectric detection channel. While the mixed liquid to be detected flows through the mixing chamber, the obstacle column in the mixing chamber breaks the cells in the mixed liquid to be detected, so that the sample to be detected and the dissolved lyophilized powder are fully mixed to improve the lysis effect, and the sample to be detected can be quickly detected. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 A schematic diagram of the structure of a prepackaged microfluidic chip provided in one embodiment of the present application;

[0034] Figure 2 for Figure 1 Schematic diagram of the structure of the mixing chamber;

[0035] Figure 3 A schematic diagram of the structure of a cell detection device provided in one embodiment of the present application;

[0036] Figure 4 for Figure 3 A schematic diagram of the cross-sectional structure of a cell detection device;

[0037] Figure 5 A schematic diagram of the structure of a device housing provided in one embodiment of the present application;

[0038] Figure 6 A schematic diagram of the structure of a carrier module provided in one embodiment of the present application;

[0039] Figure 7 A schematic diagram of the structure of a photoelectric detection module provided in one embodiment of the present application;

[0040] Figure 8 A schematic diagram of calibration results under different ATP concentrations provided in one embodiment of the present application.

[0041] Description of reference numerals:

[0042] 10-prepackaged microfluidic chip, 110-chip body, 120-circulation chamber, 130-storage chamber, 140-mixing chamber, 150-photoelectric detection channel, 160-counterweight block;

[0043] 121- sample adding port;

[0044] 141 - mixing chamber, 142 - circulation channel, 143 - first obstacle column, 144 - second obstacle column;

[0045] 151-exhaust port;

[0046] 20-device housing, 210-outer housing, 220-shading plate;

[0047] 211-viewing window, 212-assembly window, 213-sliding cover plate;

[0048] 30-control component, 310-main control board, 320-touch screen, 330-power supply;

[0049] 40-carrying module, 410-linear carrying assembly, 420-rotating assembly;

[0050] 411 - fixed base, 412 - first fixed vertical plate, 413 - second fixed vertical plate, 414 - guide rod, 415 - lead screw, 416 - first motor, 417 - carrier plate;

[0051] 421- second motor;

[0052] 50-photoelectric detection module, 510-photomultiplier tube module;

[0053] 511-Photosensitive area. DETAILED DESCRIPTION

[0054] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present application. However, the present application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present application, so the present application is not limited by the specific embodiments disclosed below.

[0055] In the description of the present application, it should be understood that if the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. appear, the orientation or position relationship indicated by these terms is based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application.

[0056] In addition, if the terms "first" or "second" appear, these terms are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of this application, if the term "plurality" appears, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0057] In this application, unless otherwise clearly specified and limited, if the terms "installed", "connected", "connected", "fixed" and the like appear, these terms should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integrated connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to the specific circumstances.

[0058] In the present application, unless otherwise clearly specified and limited, if there is a description that a first feature is "above" or "below" a second feature, etc., or similar descriptions appear, it may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "above" and "above" the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. The first feature being "below", "below" and "below" the second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0059] It should be noted that if an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be a central element. If an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. If any, the terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used in this application are for illustrative purposes only and do not represent the only implementation method.

[0060] See also Figure 1 , Figure 1 A schematic structural diagram of a prepackaged microfluidic chip in an embodiment of the present application is shown. The prepackaged microfluidic chip 10 includes a chip body 110 and a flow chamber 120 , and the flow chamber 120 is disposed on the chip body 110 .

[0061] The chip body 110 is provided with a supporting column and a photoelectric detection channel 150 , and the photoelectric detection channel 150 is provided with an exhaust port 151 .

[0062] The circulation chamber 120 includes a storage chamber 130 and a mixing chamber 140. The storage chamber 130 is provided with a sample addition port 121, and freeze-dried powder is stored in the storage chamber 130, and an obstacle column is provided in the mixing chamber 140. The freeze-dried powder includes a lysate and ATPase. The mixing chamber 140 is respectively connected to the photoelectric detection channel 150 of the storage chamber 130, and the storage chamber 130 abuts against the support column.

[0063] When the prepackaged microfluidic chip 10 is placed in the detection device, the liquid to be tested is introduced into the storage chamber 130 through the sample injection port 121 of the circulation chamber 120. The lyophilized powder pre-added in the storage chamber 130 will dissolve rapidly after contacting the liquid to be tested, thereby forming a mixed liquid to be tested. In one embodiment, the liquid to be tested includes but is not limited to milk, body fluid and cell culture fluid.

[0064] After the dissolution is completed in the storage chamber 130, the mixed liquid to be tested is guided into the mixing chamber 140. At this time, the microstructure and design in the microfluidic chip ensure that the liquid is fully mixed during the flow process, avoiding uneven distribution of sample components. And the obstacle column can effectively break the cells in the process of liquid flow. The cell breaking process helps to release the components inside the cell, so that the target analyte (such as DNA, protein, ATP, metabolites, etc.) can be released into the solution, which is convenient for subsequent photoelectric detection.

[0065] Eight test points are randomly selected on the photoelectric detection channel 150 and sampled, and then an average value of the eight sample points is calculated, and then the ATP level in the sample is obtained by comparing with the calibration value.

[0066] In the above-mentioned prepackaged microfluidic chip 10, the sample to be tested, i.e., the liquid to be tested, is added from the sample addition port 121 of the storage chamber 130 in the flow chamber 120. After the liquid to be tested enters the storage chamber 130, the stored freeze-dried powder is dissolved to obtain a mixed liquid to be tested. By operating the prepackaged microfluidic chip 10, the mixed liquid to be tested flows through the mixing chamber 140 to enter the photoelectric detection channel 150. While the mixed liquid to be tested flows through the mixing chamber 140, the obstacle column in the mixing chamber 140 breaks the cells in the mixed liquid to be tested, so that the sample to be tested and the dissolved freeze-dried powder are fully mixed to improve the lysis effect, and the sample to be tested can be quickly detected.

[0067] See also Figure 2 , Figure 2 Shows Figure 1The schematic diagram of the structure of the mixing chamber 140 is shown in FIG. 1 . The mixing chamber 140 includes a mixing chamber 141 and a circulation channel 142 . The mixing chamber 141 is communicated with the storage chamber 130 , and the mixing chamber 141 is connected with the photoelectric detection channel 150 through the circulation channel 142 .

[0068] The obstacle column comprises a first obstacle column 143 . The first obstacle column 143 is disposed in the mixing chamber 141 . The cross section of the first obstacle column 143 is olive-shaped.

[0069] In the process of the mixed liquid to be tested being guided into the mixing chamber 140, the mixed liquid to be tested flows through the mixing chamber 141, and the flow of the mixed liquid to be tested is disturbed by the first obstacle column 143 in the mixing chamber 141 to increase the flow resistance, thereby strengthening the interaction between the liquid and the inner wall of the chip, that is, the flow velocity change and local turbulence of the mixed liquid to be tested when flowing through the obstacle column improve the mixing efficiency of the mixed liquid to be tested, so that the components of the mixed liquid to be tested can be fully mixed in a short time.

[0070] Continue reading Figure 2 The obstacle column further includes a second obstacle column 144 . The second obstacle column 144 is disposed in the flow channel 142 . The cross section of the second obstacle column 144 is in the shape of a water drop. The tip of the second obstacle column 144 faces the mixing chamber 141 .

[0071] As the mixed liquid to be tested continues to flow, the mixed liquid to be tested enters the circulation channel 142. The cross section of the second obstacle column 144 in the circulation channel 142 is in the shape of a water drop, and its tip faces the mixing chamber 141, forming a local obstacle in the flow. Therefore, the second obstacle column 144 further breaks the cells in the mixed liquid to be tested, and the water drop-shaped second obstacle column 144 optimizes the flow pattern of the mixed liquid to be tested, ensuring that the cell breaking is more uniform and effective.

[0072] Continue reading Figure 1 If a flow chamber 120 is provided on the prepackaged microfluidic chip 10, the prepackaged microfluidic chip 10 further includes a counterweight 160, which is disposed on the chip body 110 and is disposed on the other side of the support column relative to the flow chamber 120. The center of gravity of the prepackaged microfluidic chip 10 is adjusted to the center by the counterweight 160.

[0073] If at least two flow chambers 120 are disposed on the prepackaged microfluidic chip 10 , the plurality of flow chambers 120 are evenly spaced and distributed on the chip body 110 , so as to adjust the center of gravity of the prepackaged microfluidic chip 10 to the center.

[0074] In an exemplary embodiment, the present application provides a prepackaged microfluidic chip 10 , which includes a chip body 110 and a flow chamber 120 , wherein the flow chamber 120 is disposed on the chip body 110 .

[0075] The chip body 110 is provided with a supporting column, a photoelectric detection channel 150 and a counterweight 160 , and the photoelectric detection channel 150 is provided with an exhaust port 151 .

[0076] The circulation chamber 120 includes a storage chamber 130 and a mixing chamber 140. The storage chamber 130 is provided with a sample addition port 121, and freeze-dried powder is stored in the storage chamber 130. The freeze-dried powder includes a lysate and ATPase. The mixing chamber 140 is communicated with the photoelectric detection channel 150 of the storage chamber 130, respectively, and the storage chamber 130 abuts against the support column. The counterweight 160 is placed on the chip body 110, and the counterweight 160 is placed on the other side of the support column relative to the circulation chamber 120.

[0077] The mixing chamber 140 includes a mixing chamber 141 and a circulation channel 142. An obstacle column is arranged in the mixing chamber 140. The mixing chamber 141 is communicated with the storage chamber 130, and the mixing chamber 141 is connected with the photoelectric detection channel 150 through the circulation channel 142. The obstacle column includes a first obstacle column 143 and a second obstacle column 144. The first obstacle column 143 is arranged in the mixing chamber 141, and the second obstacle column 144 is arranged in the circulation channel 142. The cross section of the first obstacle column 143 is olive-shaped, and the cross section of the second obstacle column 144 is drop-shaped, and the tip of the second obstacle column 144 faces the mixing chamber 141.

[0078] The liquid to be tested enters the storage chamber 130 through the sample addition port 121 of the circulation chamber 120, and dissolves rapidly after contacting the pre-added lyophilized powder to form a mixed liquid to be tested. At this time, the lyophilized powder dissolution reaction in the storage chamber 130 provides sufficient reaction power, so that the required components (such as DNA, protein, etc.) in the liquid to be tested can be released, preparing for subsequent testing.

[0079] After the mixed liquid to be tested enters the mixing chamber 140, the design inside the microfluidic chip ensures that the liquid is fully mixed. The first obstacle column 143 in the mixing chamber 141 disturbs the flow of the liquid and increases the flow resistance. In this process, the change in liquid flow rate and the formation of local turbulence effectively improve the mixing efficiency, so that the components can be quickly and evenly mixed.

[0080] As the mixed liquid to be tested continues to flow, the design of the second obstacle column 144 further optimizes the cell disruption process. The obstacle column is in the shape of a water droplet, with the tip facing the mixing chamber 141, forming a local flow barrier and enhancing the uniformity of the liquid flow. Afterwards, the mixed liquid to be tested flows into the photoelectric detection channel 150.

[0081] Eight test points are randomly selected on the photoelectric detection channel 150 and sampled, and then an average value of the eight sample points is calculated, and then the ATP level in the sample is obtained by comparing with the calibration value.

[0082] See also Figure 3 and Figure 4 , Figure 3 FIG. 1 shows a schematic diagram of the structure of a cell detection device provided in an embodiment of the present application. Figure 4 Shows Figure 3 Schematic diagram of the cross-sectional structure of a cell detection device. The cell detection device comprises the pre-packaged microfluidic chip 10, the device housing 20, the control component 30, the carrier module 40 and the photoelectric detection module 50 described in any of the above embodiments, the control component 30 is arranged on the inner side wall of the device housing 20, the carrier module 40 is arranged on the inner bottom of the device housing 20, the pre-packaged microfluidic chip 10 is arranged on the carrier module 40, and the photoelectric detection module 50 is arranged on the inner side of the device housing 20.

[0083] After the sample to be tested is added to the prepackaged microfluidic chip 10 through the sample addition port 121, the sample to be tested contacts the pre-added freeze-dried powder and dissolves rapidly to form a mixed liquid to be tested. The prepackaged microfluidic chip 10 with the added sample is placed on the carrier module 40, and the carrier module 40 is operated by operating the control component 30, so that the mixed liquid to be tested flows from the storage chamber 130 through the mixing chamber 140. The obstacle column in the mixing chamber disturbs the flow of the liquid, enhances the mixing efficiency, and optimizes the cell disruption process. After that, the mixed liquid to be tested enters the photoelectric detection channel 150, and then the photoelectric detection module 50 detects the mixed liquid to be tested in the photoelectric detection channel 150, and by randomly sampling multiple test points and calculating the average value, the ATP level in the sample is measured in combination with the calibration value, and the detection result is transmitted to the control component 30.

[0084] Continue reading Figure 3 and Figure 4 The control component 30 includes a main control board 310, a touch screen 320 and a power supply 330. The main control board 310 is arranged on the inner side of the second side panel of the outer shell 210, the touch screen 320 is embedded in the assembly window 212, and the touch screen 320 is connected to the main control board 310. The power supply 330 is arranged below the main control board 310, and the power supply 330 is connected to the main control board 310.

[0085] See also Figure 5 , Figure 5 The schematic diagram of the structure of the device housing 20 provided in one embodiment of the present application is shown, and the device housing 20 includes an outer shell 210 and a shading plate 220, the shading plate 220 is arranged inside the outer shell 210, the shading plate 220 is slidably connected to the inner rear plate and the right side plate of the outer shell 210 respectively, and the shading plate 220 divides the inner part of the outer shell 210 into a shading area and a carrying area. The outer shell 210 includes a viewing window 211, an assembly window 212 and a sliding cover plate 213, the viewing window 211 and the assembly window 212 are both arranged on the front-view shell plate of the outer shell 210, and the sliding cover plate 213 is embedded in the first side plate of the outer shell 210.

[0086] During the loading process of the pre-packaged microfluidic chip 10 , the operator opens the sliding cover 213 to load the sample, and then closes the sliding cover 213 immediately after completion to ensure that the inside of the platform remains dark during the detection process.

[0087] In one embodiment, the front-view shell plate includes a lower shell plate and an upper shell plate, the lower shell plate is perpendicular to the bottom surface of the outer shell 210, and the upper shell plate and the lower shell plate are arranged at an angle; the viewing window 211 is arranged on the upper shell plate, and the assembly window 212 is arranged on the lower shell plate.

[0088] See also Figure 6 , Figure 6 A schematic structural diagram of a transport module 40 provided in an embodiment of the present application is shown. The transport module 40 includes a linear transport component 410 and a rotating component 420 . The linear transport component 410 is disposed at the inner bottom of the outer shell 210 .

[0089] The linear transport assembly 410 includes a fixed base 411, a first fixed vertical plate 412, a second fixed vertical plate 413, a guide rod 414, a lead screw 415, a first motor 416 and a carrier plate 417. The fixed base 411 is arranged at the inner bottom of the outer shell 210, the first fixed vertical plate 412 and the second fixed vertical plate 413 are arranged at two ends of the fixed base 411 respectively, the guide rod 414 is connected between the first fixed vertical plate 412 and the second fixed vertical plate 413, the first end of the lead screw 415 passes through the first fixed vertical plate 412, and the second end of the lead screw 415 is connected to the second fixed vertical plate 413, the first motor 416 is arranged outside the first fixed vertical plate 412, and the first motor 416 is connected to the first end of the lead screw 415 through a coupling, and the guide rod 414 and the lead screw 415 both pass through the carrier plate 417. In one embodiment, the fixed base 411 is made of 2060 aluminum profile.

[0090] Two guide rods 414 are disposed between the first fixed vertical plate 412 and the second fixed vertical plate 413 . The two guide rods 414 and the lead screw 415 are located in the same plane, and the two guide rods 414 are symmetrically disposed with respect to the lead screw 415 .

[0091] The rotating assembly 420 is placed on the carrier plate 417 . The rotating assembly 420 includes a second motor 421 . The second motor 421 is fixedly connected to the carrier plate 417 . The pre-packaged microfluidic chip 10 is connected to the rotating shaft of the second motor 421 .

[0092] The rotating shafts of the first motor 416 and the second motor 421 are both D-shaped shafts. A D-shaped hole is provided at the center of the prepackaged microfluidic chip 10 , and the D-shaped hole of the prepackaged microfluidic chip 10 matches the D-shaped shaft of the second motor 421 .

[0093] See also Figure 7 , Figure 7 A structural schematic diagram of a photoelectric detection module 50 provided in an embodiment of the present application is shown, wherein the photoelectric detection module 50 includes a photomultiplier tube module 510, which is disposed on a light shielding plate 220, and the photomultiplier tube module 510 includes a photosensitive area 511, and the photosensitive area 511 faces the pre-packaged microfluidic chip 10.

[0094] In an exemplary embodiment, the present application provides a cell detection device, which includes the prepackaged microfluidic chip 10, the device housing 20, the control component 30, the carrier module 40 and the photoelectric detection module 50 described in any of the above embodiments, the control component 30 is arranged on the inner wall of the device housing 20, the carrier module 40 is arranged on the inner bottom of the device housing 20, and the prepackaged microfluidic chip 10 is arranged on the carrier module 40, and the photoelectric detection module 50 is arranged on the inner side of the device housing 20.

[0095] The control component 30 includes a main control board 310, a touch screen 320 and a power supply 330. The main control board 310 is arranged on the inner side of the second side panel of the outer shell 210, the touch screen 320 is embedded in the assembly window 212, and the touch screen 320 is connected to the main control board 310. The power supply 330 is arranged below the main control board 310, and the power supply 330 is connected to the main control board 310.

[0096] The device housing 20 includes an outer shell 210 and a shading plate 220. The shading plate 220 is arranged inside the outer shell 210. The shading plate 220 is slidably connected to the inner rear plate and the right side plate of the outer shell 210 respectively, and the shading plate 220 divides the inner part of the outer shell 210 into a shading area and a carrying area. The outer shell 210 includes a viewing window 211, an assembly window 212 and a sliding cover plate 213. The viewing window 211 and the assembly window 212 are both arranged on the front-view shell plate of the outer shell 210, and the sliding cover plate 213 is embedded in the first side plate of the outer shell 210. Among them, the front-view shell plate includes a lower shell plate and an upper shell plate. The lower shell plate is perpendicular to the bottom surface of the outer shell 210, and the upper shell plate is arranged at an angle with the lower shell plate; the viewing window 211 is arranged on the upper shell plate, and the assembly window 212 is arranged on the lower shell plate.

[0097] The transport module 40 includes a linear transport component 410 and a rotating component 420 . The linear transport component 410 is disposed at the inner bottom of the outer shell 210 .

[0098] The linear transport assembly 410 includes a fixed base 411, a first fixed vertical plate 412, a second fixed vertical plate 413, a guide rod 414, a lead screw 415, a first motor 416 and a carrier plate 417. The fixed base 411 is arranged at the inner bottom of the outer shell 210, the first fixed vertical plate 412 and the second fixed vertical plate 413 are arranged at two ends of the fixed base 411 respectively, the guide rod 414 is connected between the first fixed vertical plate 412 and the second fixed vertical plate 413, the first end of the lead screw 415 passes through the first fixed vertical plate 412, and the second end of the lead screw 415 is connected to the second fixed vertical plate 413, the first motor 416 is arranged outside the first fixed vertical plate 412, and the first motor 416 is connected to the first end of the lead screw 415 through a coupling, and the guide rod 414 and the lead screw 415 both pass through the carrier plate 417. In one embodiment, the fixed base 411 is made of 2060 aluminum profile. Two guide rods 414 are arranged between the first fixed vertical plate 412 and the second fixed vertical plate 413. The two guide rods 414 and the lead screw 415 are in the same plane, and the two guide rods 414 are symmetrically arranged about the lead screw 415. The rotating assembly 420 is placed on the carrier plate 417. The rotating assembly 420 includes a second motor 421. The second motor 421 is fixedly connected to the carrier plate 417, and the pre-packaged microfluidic chip 10 is connected to the rotating shaft of the second motor 421. Among them, the rotating shaft of the first motor 416 and the rotating shaft of the second motor 421 are both D-shaped shafts, and a D-shaped hole is arranged at the center of the pre-packaged microfluidic chip 10, and the D-shaped hole of the pre-packaged microfluidic chip 10 matches the D-shaped shaft of the second motor 421.

[0099] The ATP standard sample was prepared into a 300 μmol / L solution and diluted stepwise to obtain ATP solutions with concentrations of 250, 200, 150, 100 and 50 μmol / L, and then 100 μL of each concentration solution was taken. The solutions of different concentrations were then injected into the storage chamber 130 of the pre-packaged microfluidic chip 10. The obtained concentration calibration curve is shown in the attached manual. Figure 8 shown.

[0100] Then the prepackaged microfluidic chip 10 is placed on the rotating shaft of the second motor 421, and the push-pull cover 213 is closed to obtain a dim environment. The first motor 416 is started through the touch screen of the control component 30, so that the prepackaged microfluidic chip 10 is carried to the detection area. Then the second motor 421 is started to make the prepackaged microfluidic chip 10 centrifugal rotate, so that the liquid is fully mixed in the mixing chamber 140 of the prepackaged microfluidic chip 10 to react and generate a light signal, and the liquid enters the photoelectric detection channel 150. The photomultiplier tube detects the ATP biological light signal, converts the light signal into an electrical signal, and reacts the electrical signal to the touch screen through the ADC sampling function of the main control board 310, so as to obtain the corresponding light intensity, and the number of cells or bacteria in the liquid to be tested can be obtained according to the light intensity.

[0101] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0102] The above-described embodiments only express several implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be construed as limiting the scope of the patent application. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the protection scope of the patent application shall be subject to the attached claims.

Claims

1. A prepackaged microfluidic chip, characterized in that: include: The chip body includes a support column and a photoelectric detection channel, wherein the photoelectric detection channel is provided with an exhaust port; A flow chamber, wherein the flow chamber is arranged on the chip body and is connected to the photoelectric detection channel, the flow chamber includes a storage chamber and a mixing chamber, the storage chamber stores freeze-dried powder for detection, and the storage chamber includes a sample addition port, the sample addition port is used to add samples to be detected, an obstacle column is arranged in the mixing chamber, the mixing chamber is connected to the photoelectric detection channel, and the storage chamber abuts the support column.

2. The prepackaged microfluidic chip according to claim 1, characterized in that: The mixing chamber comprises a mixing chamber and a circulation channel, the mixing chamber is communicated with the storage chamber, and the mixing chamber is connected with the photoelectric detection channel through the circulation channel, and the volume of the storage chamber is equal to the volume of the photoelectric detection channel; The obstacle column comprises a first obstacle column, the first obstacle column is arranged in the mixing chamber, and the cross section of the first obstacle column is olive-shaped.

3. The prepackaged microfluidic chip according to claim 2, characterized in that: The obstacle column further includes a second obstacle column, which is disposed in the flow channel. The cross section of the second obstacle column is in the shape of a water drop, and the tip of the second obstacle column faces the mixing chamber.

4. The prepackaged microfluidic chip according to claim 1, characterized in that: The prepackaged microfluidic chip further comprises a counterweight block, which is disposed on the chip body and on the other side of the support column relative to the flow chamber.

5. A cell detection device, characterized in that: The prepackaged microfluidic chip comprises any one of claims 1 to 4, further comprising: Device housing; A control assembly, the control assembly being disposed on an inner side wall of the device housing; A carrier module, the carrier module is placed at the inner bottom of the device housing, and the prepackaged microfluidic chip is placed on the carrier module; A photoelectric detection module is arranged inside the device housing.

6. The cell detection device according to claim 5, characterized in that: The device housing comprises: The outer shell comprises a viewing window, an assembly window and a sliding cover plate, wherein the viewing window and the assembly window are both arranged on the front-view shell plate of the outer shell, and the sliding cover plate is embedded in the first side plate of the outer shell; A shading plate is arranged inside the outer shell, the shading plate is slidably connected to the inner rear plate and the right side plate of the outer shell respectively, and the photoelectric detection module is arranged on the shading plate.

7. The cell detection device according to claim 6, characterized in that: The front-view shell plate comprises a lower shell plate and an upper shell plate, wherein the lower shell plate is perpendicular to the bottom surface of the outer shell, and the upper shell plate is arranged at an angle with the lower shell plate; The viewing window is arranged on the upper shell plate, and the assembly window is arranged on the lower shell plate.

8. The cell detection device according to claim 6, characterized in that: The control components include: A main control board, the main control board is arranged on the inner side of the second side plate of the outer shell; A touch screen, which is embedded in the assembly window and connected to the main control board; A power supply component is arranged below the main control board and is connected to the main control board.

9. The cell detection device according to claim 6, characterized in that: The carrier module includes: A linear transport assembly, the linear transport assembly is arranged at the inner bottom of the outer shell, the linear transport assembly comprises a fixed base, a first fixed vertical plate, a second fixed vertical plate, a guide rod, a lead screw, a first motor and a carrier plate, the fixed base is arranged at the inner bottom of the outer shell, the first fixed vertical plate and the second fixed vertical plate are arranged at two ends of the fixed base respectively, a guide rod is connected between the first fixed vertical plate and the second fixed vertical plate, the first end of the lead screw passes through the first fixed vertical plate, the second end of the lead screw is connected to the second fixed vertical plate, the first motor is arranged at the outer side of the first fixed vertical plate, and the first motor is connected to the first end of the lead screw through a coupling, and the guide rod and the lead screw both pass through the carrier plate; A rotating assembly is arranged on the carrier plate, and the rotating assembly comprises a second motor, the second motor is fixedly connected to the carrier plate, and the pre-packaged microfluidic chip is connected to the rotating shaft of the second motor.

10. The cell detection device according to claim 6, characterized in that: Photoelectric detection module includes: A photomultiplier tube module is arranged on the light shielding plate, and comprises a photosensitive area, and the photosensitive area faces the pre-packaged microfluidic chip.

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