System and method for fast focusing in combination with Raman laser spots

Abstract

The invention provides a system and a method for fast focusing in combination with Raman laser spots, and belongs to the technical field of optical imaging, the system comprises a spectrum excitation light path, a spectrum receiving light path, an imaging light path and a control system; the spectrum excitation light path, the spectrum receiving light path and the imaging light path jointly form a Raman focusing light path; through fitting calibration preparation of the optimal focal plane, judgment basis of the aggregation fitting process, setting of the aggregation fitting process and starting of the automatic focusing process, rapid focusing is realized, aggregation calculation can be carried out only by reading the laser spot image at the current position, the automatic focusing efficiency is greatly improved, and the automatic focusing precision is improved. The method is of great significance to various applications needing Raman splicing and Mapping, and the overall technical scheme is scientific and reliable in design and suitable for application and popularization.

Description

Technical Field

[0001] The invention belongs to the technical field of optical imaging, and in particular to a system and method for rapid focusing in combination with a Raman laser spot. Background Art

[0002] In Raman confocal microscopy, achieving fast and accurate autofocus is the key to improving imaging quality and efficiency.

[0003] Traditional Raman confocal systems rely on complex mechanical adjustments and preset focusing modes for automatic focusing. These methods have limitations in speed and accuracy, such as: ① Automatic focusing method based on image grayscale: This method collects multiple images and analyzes the grayscale distribution of the images to determine the focusing state; It requires the acquisition and processing of multiple images, has a complex algorithm, and cannot give the defocus direction and distance based on just one image, making it unsuitable for high-speed real-time systems.

[0004] ② Cylindrical lens and four-quadrant detector method: This method determines the focusing state by changing the shape of the reflected light spot; It is greatly affected by ambient light, and its focusing precision and accuracy are not ideal.

[0005] In order to overcome the defects of the above-mentioned automatic focusing mode, the prior art proposes a solution to achieve automatic focusing with the help of laser technology, for example: ① An automatic positioning method based on the spot area: through the introduction of the structure of the laser focusing lens, laser ranging and other technologies are used to calculate and locate the spot area; However, in laser induced breakdown spectroscopy (LIBS) technology, due to the different thicknesses and surface flatness of the samples to be measured, the relative distance between the laser focusing lens and the sample surface will vary greatly, ultimately affecting the stability and accuracy of the measurement results.

[0006] ② There is also a laser confocal system that can accurately locate and read optical signals of tiny targets. The system needs to automatically focus to obtain a clear image, thereby further acquiring information about tiny targets. However, since the target is tiny, in order to see the tiny target clearly, the objective lens is usually more than 50 times; the imaging area of ​​the objective lens at this magnification is small, the depth of field is small, and it is very easy to be out of focus, so it is necessary to perform multiple automatic focusing when the field of view is changed or when working for a long time, which is cumbersome to operate.

[0007] In summary, the current automatic focusing adjustment methods, whether manual adjustment, mechanical adjustment, adjustment based on the imaging clarity of the sample surface, or adjustment using a laser rangefinder, are not very accurate and are greatly affected by the surface characteristics of the sample; in application scenarios that require rapid switching of focus or imaging in a dynamic environment, the above-mentioned existing technical solutions are difficult to meet the requirements of high efficiency and high precision. Summary of the invention

[0008] In order to solve the above technical problems, the present invention provides a system and method for rapid focusing in combination with a Raman laser spot, which overcomes the defects of the automatic focusing method in the prior art that is complex and inefficient, and greatly improves the focusing speed and accuracy of the optical imaging system.

[0009] A system and method for rapid focusing in combination with a Raman laser spot, wherein: In a first aspect, a system for rapid focusing in combination with a Raman laser spot comprises: Spectral excitation optical path, spectral receiving optical path, imaging optical path and control system; As an example, the spectral excitation light path, the spectral receiving light path and the imaging light path together constitute a Raman focusing light path.

[0010] The spectral excitation optical path includes: a laser, a dichroic mirror, an objective lens and a light shield; As an example, the color of the laser beam emitted by the laser is green.

[0011] The spectrum receiving optical path includes: an optical fiber, a spectrum detector, and a reflector; the spectrum detector transmits the collected spectrum data to a computer; As an example, the spectrum detector adopts: a spectrometer; The imaging optical path includes: a beam splitter and an imaging detector; the imaging detector transmits the collected image data to a computer; the computer is electrically connected to the controller to form signal communication; As an example, the imaging detector adopts: a CMOS camera.

[0012] As an example, the imaging optical path realizes the position imaging of the Raman spot through the architecture design of the splitting optical path, and the position imaging and the imaging size are used to perform aggregation judgment.

[0013] The control system includes: a computer, a controller, an XY stage and a Z-axis displacement mechanism.

[0014] Further, the reflector is arranged on the left side of the optical fiber, and the optical fiber is arranged on the left side of the spectrum detector; the dichroic mirror is arranged directly below the reflector; The laser is arranged on the right side of the dichroic mirror, and the light shielding plate is arranged at a position slightly below the middle of the laser and the dichroic mirror; The beam splitter is arranged directly below the dichroic mirror, and the imaging detector is arranged on the right side of the dichroic mirror; The objective lens is arranged directly below the beam splitter; an XY stage is arranged below the objective lens, a Z-axis displacement mechanism is arranged below the XY stage, and the XY stage is used for placing a glass slide.

[0015] As an example, the glass slide is used to store the sample to be tested.

[0016] As an example, the specification of the slide is preferably: 75*25mm.

[0017] As an example, the test targets in the sample include cells, bacteria and particles, and the minimum size of the test target can be 0.5 μm.

[0018] In a second aspect, a method for rapid focusing in combination with a Raman laser spot is provided, wherein rapid focusing is achieved through an automatic focusing process, including: Step 1: Preparation for fitting and calibration of the best focal plane; The slide is placed on the XY stage, and the image data detected by the imaging detector is obtained in real time by the computer; As an example, when performing fitting calibration of the best focal plane, the slide may contain a sample or be blank.

[0019] Step 2: Judgment basis of the aggregation fitting process; ① When the Z-axis displacement mechanism is at the optimal gathering position Z f hour; At this time, the image data obtained by the computer is: a green laser spot, which is circular in shape, has the largest radius and the smallest area; ② When the Z-axis displacement mechanism is at the optimal gathering position Z f When above ; At this time, the image data obtained by the computer is: a green laser spot, which is in the shape of a left semicircle and has a larger area; ③ When the Z-axis displacement mechanism is at the optimal gathering position Z f When below ; At this time, the image data obtained by the computer is: a green laser spot, which is in the shape of a right semicircle and has a larger area; Step 3: Setting of the aggregation fitting process; ① Collect image data sequences at different Z-axis displacement mechanism height positions; The height positions of the Z-axis displacement mechanism are respectively: Z0, Z1...Zn, and the green laser spot area corresponding to each height position to obtain image data is: S0, S1...Sn; As an example, during the focus fitting calculation, the computer will obtain a camera image by collecting images at different positions of the Z axis. Due to the Raman laser, a green spot appears in the image. The area of ​​the green laser spot is calculated to be S0, S1...Sn. The area of ​​the green laser spot calculated by collecting the image at the first position of the Z axis is S0.

[0020] Since the fitting calculation uses a quadratic polynomial, theoretically, collecting 4 images with different Z axes can be used for fitting calculation. In order to fit more accurately, this application collects 8 images with different Z axes and calculates the area S value. At this time, n=7, Sn=S7.

[0021] ② When the green laser spot area is the smallest, the corresponding height position is recorded as Z f , the area is recorded as: S f ; ③ Relative Z0...Zn to Z f The position difference is recorded as a Y sequence: Y0...Yn; As an example, in the Y sequence: Y0=Z0-Z f , Yn=Zn-Z f ; ④ Relative green laser spot area S0...Sn to S f The area difference is recorded as X sequence: X0...Xn; As an example, in the X sequence, X0=S0-S f ,Xn=S n -S f .

[0022] ⑤ Establish a mapping from the X sequence to the Y sequence, obtain the aggregation: Y=f(X), and perform fitting calculation; As an example, the mapping is fitted using the least squares method, and the fitting formula is designed as follows: ; According to the least squares principle: ,

[0023] The coefficients a2, a1, and a0 can be solved.

[0024] As an example, the fitting calculation is not limited to the fitting of a quadratic polynomial, but may also be a cubic spline interpolation.

[0025] Step 4: After the fitting calculation is completed, the fitting formula is stored in the computer system; in actual work, when the field of view is switched or the working time in the same field of view exceeds the time threshold, the computer system issues a command to start the above-mentioned automatic focusing process to achieve rapid focusing.

[0026] As an example, the automatic focusing process is: collect the laser Raman image of the test target, and calculate the real-time green laser spot area as: X now , X now Input aggregation Y=f(X), get aggregation Y now , move the Z-axis displacement mechanism relative to the gathering Y now Location is fine.

[0027] As an example, the time threshold refers to a waiting time value scientifically set according to actual usage. The time threshold can be adjusted and updated according to the usage environment.

[0028] In a third aspect, the present application shows a non-temporary computer-readable storage medium, when the instructions in the storage medium are executed by a processor of an electronic device, the electronic device is enabled to execute the method described in any of the above aspects.

[0029] In a fourth aspect, the present application illustrates a computer program product. When instructions in the computer program product are executed by a processor of an electronic device, the electronic device is enabled to execute the method described in any of the above aspects.

[0030] Beneficial effects of the present invention: This method only needs to read the laser spot image at the current position to perform focusing calculations. Previous methods require moving multiple Z-axis steps and performing image acquisition and image clarity calculations before achieving one focusing. Therefore, the technical solution of this application greatly improves the automatic focusing efficiency, which is of great significance for various applications that require Raman stitching and mapping.

[0031] The present invention has fast automatic focusing, timely response, time saving, higher efficiency than the prior art, stable and reliable operation, and is suitable for popularization and application. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 This is a diagram of the overall structure of a system for rapid focusing in combination with a Raman laser spot according to the present invention.

[0033] Figure 2 The figure is a schematic diagram of the Raman focusing optical path structure of a system for fast focusing in combination with a Raman laser spot according to the present invention.

[0034] Figure 3The figure is a schematic diagram of the process design of a method for rapid focusing in combination with a Raman laser spot according to the present invention. DETAILED DESCRIPTION

[0035] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without making creative work belong to the scope of protection of the present application, and reference is made to the accompanying drawings in the embodiments of the present application. Figures 1 to 3 As shown; A system and method for rapid focusing in combination with a Raman laser spot, wherein: In a first aspect, a system for rapid focusing in combination with a Raman laser spot comprises: Spectral excitation optical path, spectral receiving optical path, imaging optical path and control system; As an example, the spectral excitation light path, the spectral receiving light path and the imaging light path together constitute a Raman focusing light path.

[0036] The spectral excitation optical path includes: a laser 1, a dichroic mirror 2, an objective lens 3 and a light shielding plate 4; As an example, the color of the laser beam emitted by the laser 1 is green.

[0037] The spectrum receiving optical path includes: an optical fiber 5, a spectrum detector, and a reflector 7; the spectrum detector transmits the collected spectrum data to a computer 10; As an example, the spectrum detector adopts: a spectrometer 6; The imaging optical path includes: a beam splitter 8 and an imaging detector 9; the imaging detector 9 transmits the collected image data to a computer 10; the computer 10 is electrically connected to the controller 11 to form signal communication; As an example, the imaging detector 9 adopts: a CMOS camera.

[0038] As an example, the imaging optical path realizes the position imaging of the Raman spot through the architecture design of the splitting optical path, and the position imaging and the imaging size are used to perform aggregation judgment.

[0039] The control system includes: a computer 10 , a controller 11 , an XY stage 12 and a Z-axis displacement mechanism 13 .

[0040] Further, the reflector 7 is arranged on the left side of the optical fiber 5, and the optical fiber 5 is arranged on the left side of the spectrum detector 6; the dichroic mirror 2 is arranged directly below the reflector 7; The laser 1 is arranged on the right side of the dichroic mirror 2, and the light shielding plate 4 is arranged at a position slightly below the middle of the laser 1 and the dichroic mirror 2; The beam splitter 8 is arranged directly below the dichroic mirror 2, and the imaging detector 9 is arranged on the right side of the dichroic mirror 2; The objective lens 3 is arranged directly below the beam splitter 8; an XY stage 12 is arranged below the objective lens 3, a Z-axis displacement mechanism 13 is arranged below the XY stage 12, and the XY stage 12 is used to place a glass slide.

[0041] As an example, the glass slide is used to store the sample to be tested.

[0042] As an example, the specification of the slide is preferably: 75*25mm.

[0043] As an example, the test targets in the sample include cells, bacteria and particles, and the minimum size of the test target can be 0.5 μm.

[0044] In a second aspect, a method for rapid focusing in combination with a Raman laser spot is provided, wherein rapid focusing is achieved through an automatic focusing process, including: Step 1: Preparation for fitting and calibration of the best focal plane; The slide is placed on the XY stage 12, and the image data detected by the imaging detector 9 is obtained in real time by the computer 10; As an example, when performing fitting calibration of the best focal plane, the slide may contain a sample or be blank.

[0045] Step 2: Judgment basis of the aggregation fitting process; ① When the position of the Z-axis displacement mechanism 13 is at the optimal gathering position Z f hour; At this time, the image data obtained by the computer 10 is: a green laser spot, which is circular in shape, has the largest radius and the smallest area; ② When the Z-axis displacement mechanism 13 is at the optimal gathering position Z f When above ; At this time, the image data obtained by the computer 10 is: a green laser spot, which is in the shape of a left semicircle and has a larger area; ③ When the Z-axis displacement mechanism 13 is at the optimal gathering position Z f When below ; At this time, the image data obtained by the computer 10 is: a green laser spot, which is in the shape of a right semicircle and has a larger area; Step 3: Setting of the aggregation fitting process; ① Collecting image data sequences at different height positions of the Z-axis displacement mechanism 13; The height positions of the Z-axis displacement mechanism 13 are respectively: Z0, Z1...Zn, and the green laser spot area corresponding to each height position to obtain image data is: S0, S1...Sn; As an example, during the focus fitting calculation, the Z-axis displacement mechanism 13 performs image acquisition at different positions and the computer will obtain a camera image. Due to the Raman laser, a green spot appears in the image. The green laser spot area is calculated to obtain S0, S1...Sn. The green laser spot area calculated by collecting the image at the first position of the Z axis is S0.

[0046] Since the fitting calculation uses a quadratic polynomial, theoretically, collecting 4 images with different Z axes can be used for fitting calculation. In order to fit more accurately, this application collects 8 images with different Z axes and calculates the area S value. At this time, n=7, Sn=S7.

[0047] ② When the green laser spot area is the smallest, the corresponding height position is recorded as Z f , the area is recorded as: S f ; ③ Relative Z0...Zn to Z f The position difference is recorded as a Y sequence: Y0...Yn; As an example, in the Y sequence: Y0=Z0-Z f , Yn=Zn-Z f ; ④ Relative green laser spot area S0...Sn to S f The area difference is recorded as X sequence: X0...Xn; As an example, in the X sequence, X0=S0-S f ,Xn=S n -S f .

[0048] ⑤ Establish a mapping from the X sequence to the Y sequence, obtain the aggregation: Y=f(X), and perform fitting calculation; As an example, the mapping is fitted using the least squares method, and the fitting formula is designed as follows: ; According to the least squares principle: ,

[0049] The coefficients a2, a1, and a0 can be solved.

[0050] As an example, the fitting calculation is not limited to the fitting of a quadratic polynomial, but may also be a cubic spline interpolation.

[0051] Step 4: After the fitting calculation is completed, the fitting formula is stored in the computer 10 system; in actual work, when the field of view is switched or the working time in the same field of view exceeds the time threshold, the computer system issues an instruction to start the above-mentioned automatic focusing process to achieve fast focusing.

[0052] As an example, the automatic focusing process is: collect the laser Raman image of the test target, and calculate the real-time green laser spot area as: X now , X now Input aggregation Y=f(X), get aggregation Y now , the Z-axis displacement mechanism 13 moves relatively to the gathering Y now Location is fine.

[0053] As an example, the time threshold refers to a waiting time value scientifically set according to actual usage. The time threshold can be adjusted and updated according to the usage environment.

[0054] In a third aspect, the present application shows a non-temporary computer-readable storage medium, when the instructions in the storage medium are executed by a processor of an electronic device, the electronic device is enabled to execute the method described in any of the above aspects.

[0055] In a fourth aspect, the present application illustrates a computer program product. When instructions in the computer program product are executed by a processor of an electronic device, the electronic device is enabled to execute the method described in any of the above aspects.

[0056] It should be noted that, for the method embodiments, for the sake of simplicity, they are all expressed as a series of action combinations, but those skilled in the art should be aware that the present application is not limited by the order of the actions described, because according to the present application, certain steps can be performed in other orders or simultaneously. Secondly, those skilled in the art should also be aware that the embodiments described in the specification are all optional embodiments, and the actions involved are not necessarily required by the present application.

[0057] It should be noted that, in this article, the terms "include", "comprises" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, an element defined by the sentence "comprises a ..." does not exclude the existence of other identical elements in the process, method, article or device including the element.

[0058] Through the description of the above implementation methods, those skilled in the art can clearly understand that the above-mentioned embodiment methods can be implemented by means of software plus a necessary general hardware platform, and of course by hardware, but in many cases the former is a better implementation method. Based on such an understanding, the technical solution of the present application, or the part that contributes to the prior art, can be embodied in the form of a software product, which is stored in a storage medium (such as ROM / RAM, a magnetic disk, or an optical disk), and includes a number of instructions for a terminal (which can be a mobile phone, a computer, a server, an air conditioner, or a network device, etc.) to execute the methods described in each embodiment of the present application.

[0059] The embodiments of the present application are described above in conjunction with the accompanying drawings, but the present application is not limited to the above-mentioned specific implementation methods. The above-mentioned specific implementation methods are merely illustrative and not restrictive. Under the guidance of the present application, ordinary technicians in this field can also make many forms without departing from the purpose of the present application and the scope of protection of the claims, all of which are within the protection of the present application.

[0060] Those of ordinary skill in the art will appreciate that the units and algorithm steps of each example described in conjunction with the embodiments disclosed in the present application can be implemented in electronic hardware, or a combination of computer software and electronic hardware. Whether these functions are performed in hardware or software depends on the specific application and design constraints of the technical solution. Professional and technical personnel can use different methods to implement the described functions for each specific application, but such implementation should not be considered to be beyond the scope of this application.

[0061] Those skilled in the art can clearly understand that, for the convenience and brevity of description, the specific working processes of the systems, devices and units described above can refer to the corresponding processes in the aforementioned method embodiments and will not be repeated here.

[0062] In the embodiments provided in the present application, it should be understood that the disclosed devices and methods can be implemented in other ways. For example, the device embodiments described above are only schematic. For example, the division of the units is only a logical function division. There may be other division methods in actual implementation, such as multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. Another point is that the mutual coupling or direct coupling or communication connection shown or discussed can be through some interfaces, indirect coupling or communication connection of devices or units, which can be electrical, mechanical or other forms.

[0063] The units described as separate components may or may not be physically separated, and the components shown as units may or may not be physical units, that is, they may be located in one place or distributed on multiple network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the solution of this embodiment.

[0064] In addition, each functional unit in each embodiment of the present application may be integrated into one processing unit, or each unit may exist physically separately, or two or more units may be integrated into one unit.

[0065] If the functions are implemented in the form of software functional units and sold or used as independent products, they can be stored in a computer-readable storage medium. Based on this understanding, the technical solution of the present application, or the part that contributes to the prior art or the part of the technical solution, can be embodied in the form of a software product, which is stored in a storage medium and includes several instructions for a computer device (which can be a personal computer, server, or network device, etc.) to perform all or part of the steps of the methods described in each embodiment of the present application. The aforementioned storage medium includes: various media that can store program codes, such as USB flash drives, mobile hard drives, ROM, RAM, magnetic disks, or optical disks.

[0066] The above are only preferred embodiments of the present invention. It should be understood that the description of the above embodiments is only used to help understand the method and core ideas of the present invention, and is not used to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, etc. made within the ideas and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A system for rapid focusing in combination with a Raman laser spot, characterized in that: include: Spectral excitation optical path, spectral receiving optical path, imaging optical path and control system; The spectral excitation optical path, the spectral receiving optical path and the imaging optical path together constitute a Raman focusing optical path; The spectral excitation optical path includes: a laser, a dichroic mirror, an objective lens and a light shield; The spectrum receiving optical path includes: an optical fiber, a spectrum detector, and a reflector; the spectrum detector transmits the collected spectrum data to a computer; The imaging optical path includes: a beam splitter and an imaging detector; the imaging detector transmits the collected image data to a computer; the computer is electrically connected to the controller to form signal communication; The control system includes: a computer, a controller, an XY stage and a Z-axis displacement mechanism.

2. A system for rapid focusing in combination with a Raman laser spot according to claim 1, characterized in that: The imaging detector adopts: a CMOS camera; the imaging optical path realizes the position imaging of the Raman spot through the architecture design of the split light path, and the position imaging and the imaging size are used to perform aggregation judgment.

3. The system for rapid focusing in combination with Raman laser spot according to claim 1, characterized in that: The reflector is arranged on the left side of the optical fiber, and the optical fiber is arranged on the left side of the spectrum detector; the dichroic mirror is arranged directly below the reflector; the laser is arranged on the right side of the dichroic mirror, and the light shielding plate is arranged at a position slightly below the middle of the laser and the dichroic mirror; the beam splitter is arranged directly below the dichroic mirror, and the imaging detector is arranged on the right side of the dichroic mirror; the objective lens is arranged directly below the beam splitter; An XY stage is arranged below the objective lens, a Z-axis displacement mechanism is arranged below the XY stage, and the XY stage is used to place a glass slide.

4. The system for rapid focusing in combination with Raman laser spot according to claim 3, characterized in that: The glass slide is used to store the sample to be tested; the specification of the glass slide is: 75*25mm; the test targets in the sample to be tested include: cells, bacteria and particles, and the minimum size of the test target can reach 0.5μm.

5. A method for rapid focusing in combination with a Raman laser spot, characterized in that: Fast focusing is achieved through an automatic focusing process, including: Step 1: Preparation for fitting and calibration of the best focal plane; The slide is placed on the XY stage, and the image data detected by the imaging detector is obtained in real time by the computer; Step 2: Judgment basis of the aggregation fitting process; ① When the Z-axis displacement mechanism is at the optimal gathering position Z f hour; At this time, the image data obtained by the computer is: a green laser spot, which is circular in shape, has the largest radius and the smallest area; ② When the Z-axis displacement mechanism is at the optimal gathering position Z f When above ; At this time, the image data obtained by the computer is: a green laser spot in the shape of a left semicircle; ③ When the Z-axis displacement mechanism is at the optimal gathering position Z f When below ; At this time, the image data obtained by the computer is: a green laser spot in the shape of a right semicircle; Step 3: Setting of the aggregation fitting process; ① Collect image data sequences at different Z-axis displacement mechanism height positions; The height positions of the Z-axis displacement mechanism are respectively: Z0, Z1...Zn, and the green laser spot area corresponding to each height position to obtain image data is: S0, S1...Sn; ② When the green laser spot area is the smallest, the corresponding height position is recorded as Z f , the area is recorded as: S f ; ③ Relative Z0...Zn to Z f The position difference is recorded as a Y sequence: Y0...Yn; ④ Relative green laser spot area S0...Sn to S f The area difference is recorded as X sequence: X0...Xn; ⑤ Establish a mapping from the X sequence to the Y sequence, obtain the aggregation: Y=f(X), and perform fitting calculation; Step 4: After the fitting calculation is completed, the fitting formula is stored in the computer system; in actual work, when the field of view is switched or the working time in the same field of view exceeds the time threshold, the computer system issues a command to start the above-mentioned automatic focusing process to achieve rapid focusing.

6. The method for rapid focusing in combination with a Raman laser spot according to claim 5, characterized in that: When performing fitting calibration of the best focal plane, the glass slide is either with a sample or blank.

7. The method for rapid focusing in combination with a Raman laser spot according to claim 5, characterized in that: The mapping adopts the least square method for fitting calculation, and the fitting formula is designed as follows: ; According to the least squares principle: ， The coefficients a2, a1, and a0 can be solved.

8. The method for rapid focusing in combination with a Raman laser spot according to claim 7, characterized in that: The fitting calculation may also adopt a cubic spline interpolation calculation method.

9. The method for rapid focusing in combination with a Raman laser spot according to claim 5, characterized in that: The automatic focusing process is: collect the laser Raman image of the test target, and calculate the real-time green laser spot area as: X now , X now Input aggregation Y=f(X), get aggregation Y now , move the Z-axis displacement mechanism relative to the gathering Y now Location is fine.

10. The method for rapid focusing in combination with a Raman laser spot according to claim 5, characterized in that: The time threshold refers to a waiting time value scientifically set according to actual usage. The time threshold can be adjusted and updated according to the usage environment.

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