Electrochemical detector with horizontal calibration effect and use method thereof

Abstract

The invention discloses an electrochemical detector with a horizontal calibration effect and a use method thereof.The electrochemical detector comprises a microplate reader body and a supporting frame arranged below the microplate reader body, a supporting plate part is rotationally installed between the tops of the front side and the rear side of an inner cavity of the supporting frame, and a gravity guiding assembly is rotationally installed in the supporting plate part; the invention relates to the technical field of electrochemical microplate readers. According to the electrochemical detector with the horizontal calibration effect and the use method of the electrochemical detector, through cooperative arrangement of the supporting frame, the pin assembly, the supporting plate piece, the gravity guide assembly and the microplate reader body, after the supporting frame is arranged on the working table, the microplate reader body can be automatically adjusted to be in a horizontal state through traction of the gravity guide assembly; the positioning assembly is arranged, so that the positioning of the microplate reader body in the horizontal state is realized, the horizontal positioning is simple and convenient, meanwhile, the device can be suitable for working table surfaces with different inclination angles, and reliable guarantee is provided for normal use of the microplate reader body.

Description

Technical Field

[0001] The invention relates to the technical field of electrochemical enzyme markers, and in particular to an electrochemical detector with a horizontal calibration effect and a use method thereof. Background Art

[0002] The electrochemical enzyme marker uses electrochemical detection technology to convert the sample information of the screen-printed electrode into a current signal, and uses professional analysis software to calculate and analyze it to obtain the test results. It is used in conjunction with disposable screen-printed electrodes and enzyme-linked immunosorbent assay kits for the quantitative determination of the analyte in the enzyme-linked immunosorbent assay reaction substrate. When using the electrochemical enzyme marker, insert the electrode sheet into the motor socket on one side, add the detection liquid to the electrode sheet, and then start the detection.

[0003] Conventional electrochemical enzyme markers are often placed directly on a work surface for use. When the work surface is tilted, it is difficult to ensure the levelness of the electrochemical enzyme marker, that is, when the detection liquid is added, the detection liquid is prone to leakage. In addition, the electrochemical enzyme marker is directly placed on the work surface, which is easy to move during operation. Once it moves during the detection process, it is easy to affect the detection result. Based on this, an electrochemical detector with a horizontal calibration effect and a method for using the same are proposed, which can not only ensure the stable contact between the enzyme marker body and the work surface, but also maintain a horizontal state on work surfaces with different inclinations, which is convenient for the use of the enzyme marker body and provides reliable guarantee for the accuracy of the detection result. Summary of the invention

[0004] In view of the shortcomings of the prior art, the present invention provides an electrochemical detector with a horizontal calibration effect and a method of using the same, which solves the problem that the conventional electrochemical enzyme marker cannot adapt to work surfaces with different inclinations, and the electrochemical enzyme marker is directly placed on the work surface, which is easy to move during operation and easily affects the detection results.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions: an electrochemical detector with a horizontal calibration effect, comprising an ELISA instrument body and a support frame arranged below the ELISA instrument body, a support plate is rotatably installed between the tops of the front and rear sides of the inner cavity of the support frame, a gravity guide component is rotatably installed in the support plate, the ELISA instrument body is fixedly installed on the top of the gravity guide component, a positioning component is arranged on the top of the support plate, and the positioning component is used in conjunction with the gravity guide component;

[0006] The front and rear sides of the support frame are both provided with pin top assemblies, and the pin top assemblies are used in conjunction with the support plate;

[0007] A protection component is arranged at the bottom of the inner cavity of the support frame, and the protection component is used in conjunction with the pin top component and the ELISA instrument body.

[0008] The present invention is further configured as follows: the support plate member includes two mounting plates, one side of the top of each mounting plate is fixedly mounted with a hemispherical block, the two hemispherical blocks are used in conjunction with each other, and the two hemispherical blocks are fixedly connected by an angle plate;

[0009] The opposite sides of the two mounting plates are rotatably connected to the front and rear sides of the inner cavity of the support frame through pins.

[0010] The present invention is further configured as follows: the gravity guide assembly includes a ball, the top of the ball is fixedly connected to a mounting plate via a first connecting rod, the bottom of the ball is fixedly installed with a high-density weighted ball via a second connecting rod, and the weight of the high-density weighted ball is 0.5-3 times the weight of the microplate reader body;

[0011] A first mounting groove and two second mounting grooves are provided on opposite sides of the two hemispherical blocks, the first mounting groove is used in conjunction with a ball, the second mounting groove is used in conjunction with a first connecting rod and a second connecting rod respectively, and the first mounting groove is connected to the two second mounting grooves respectively;

[0012] The assembly plate is fixedly installed at the bottom of the microplate reader body, and the front and one side of the microplate reader body are respectively inlaid with a first level and a second level, and the axes of the first level and the second level are vertically crossed.

[0013] The present invention is further configured as follows: the positioning assembly comprises a positioning block, a threaded rod is passed through and threadedly installed on one side of the positioning block, and a rubber pad is fixedly installed on one end of the threaded rod;

[0014] The outer arc surfaces of the two hemispherical blocks are both provided with positioning holes, the positioning holes are connected to the first mounting groove, and the inner surfaces of the positioning holes are respectively used in conjunction with the outer peripheries of the threaded rod and the rubber pad.

[0015] The present invention is further configured as follows: a cross groove is provided at the other end of the threaded rod, a cross plate is slidably installed inside the cross groove, one end of the cross plate is fixedly connected to a connecting disk, one side of the connecting disk is fixedly connected to a rotating shaft, one end of the rotating shaft is fixedly connected to a knob, a first spring is sleeved on the outer periphery of the threaded rod, and both ends of the first spring are respectively fixedly connected to the side of the connecting disk opposite to the positioning block.

[0016] The present invention is further configured as follows: the pin top assembly includes two plug plates, the bottom of the two plug plates are rotatably mounted with a linkage plate through a rotating block, the bottom of the linkage plate is rotatably connected with an L-shaped plate through a rotating block, a slide plate is fixedly mounted at the bottom of one side of the L-shaped plate, a synchronization plate is commonly fixedly mounted at the bottom of the two slide plates, and a plurality of second springs are fixedly mounted at the bottom of the synchronization plate.

[0017] The present invention is further configured as follows: two T-slots and two slide grooves are provided on both the front and rear sides of the support frame, the two T-slots are respectively used in conjunction with the two plug plates and the linkage plate, the two slide grooves are respectively used in conjunction with the two slide plates, the top of the plug plate is slidably matched with the bottom of the mounting plate, the synchronization plate is arranged inside the support frame, and the bottom ends of the second springs are fixedly connected to the bottom of the inner cavity of the support frame;

[0018] Protective covers are fixedly installed on both the front and rear sides of the support frame. The protective covers are arranged on the top peripheries of the two plug plates, the linkage plate and the L-shaped plate. The bottom of the L-shaped plate passes through the protective cover and extends to the bottom of the protective cover.

[0019] The present invention is further configured as follows: the protection assembly includes a guard plate, a soft protection pad is fixedly installed on the top of the guard plate, a plurality of guide rods are fixedly installed on the left and right sides of the bottom of the guard plate, and a sleeve is sleeved and slidably installed on the outer periphery of the guide rod;

[0020] The sleeve is fixedly mounted on the bottom of the inner cavity of the support frame, and a third spring is sleeved on the outer periphery of the sleeve and the guide rod, and two ends of the third spring are respectively fixedly connected to the bottom of the guard plate and the bottom of the inner cavity of the support frame;

[0021] The slide plate is arranged between the guard plate and the sleeve.

[0022] The present invention is further configured as follows: a plurality of through holes communicating with the sleeve are opened at the bottom of the support frame, a suction cup is fixedly installed at the bottom of the support frame and at the periphery of the through holes, and a piston used in conjunction with the sleeve is fixedly connected to the bottom end of the guide rod;

[0023] The bottom of the support frame is also provided with an assembly hole for use with a high-density counterweight ball.

[0024] The present invention provides an electrochemical detector with a level calibration effect and a method for using the same. The invention has the following beneficial effects:

[0025] (1) The present invention coordinates the support frame, the pin assembly, the support plate, the gravity guide assembly and the ELISA instrument body. After the support frame is placed on the work surface, the ELISA instrument body can be automatically adjusted to a horizontal state by the traction of the gravity guide assembly. The positioning assembly coordinates the positioning of the ELISA instrument body to a horizontal state. The horizontal positioning is simple and convenient, and can be applied to work surfaces with different inclination angles, thereby providing reliable protection for the normal use of the ELISA instrument body.

[0026] (2) The present invention uses a pin assembly to limit the support plate on both sides, thereby ensuring the stability of the support plate and facilitating the convenient rotation of the support plate, so that the ELISA instrument body can be separated from the protective assembly when in use. When the ELISA instrument body is not in use, it is moved to the support frame, and the protective assembly covers and protects the touch screen of the ELISA instrument body to ensure its portability.

[0027] (3) The present invention cooperates with the third spring, the guide rod, the guard plate, the sleeve, the through hole, the piston and the suction cup. When the device is in use, the suction cup can be stably adsorbed on the work surface to ensure its stability. After use, the suction cup can be conveniently separated from the work surface by squeezing the guard plate. At the same time, the setting of the assembly hole provides convenient conditions for the stacking and storage of several devices, which facilitates the batch storage and transportation of the devices. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a schematic diagram of the external structure of the microplate reader of the present invention when in use;

[0029] Figure 2 It is a schematic diagram of the connection structure of the support frame, the support plate, the gravity guide assembly, the positioning assembly, the pin lift assembly and the protection assembly of the present invention;

[0030] Figure 3 It is a structural schematic diagram of the support plate member and the gravity guide assembly of the present invention;

[0031] Figure 4 It is a structural schematic diagram of the support plate member and the positioning assembly of the present invention;

[0032] Figure 5 It is a structural schematic diagram of the pin top assembly of the present invention;

[0033] Figure 6 It is a schematic diagram of the connection between the support frame and the protection component structure of the present invention;

[0034] Figure 7 It is a schematic diagram of the connection of the mounting plate, hemispherical block, angle plate and positioning block structure of the present invention;

[0035] Figure 8 It is a schematic diagram of the external structure of the ELISA instrument body of the present invention when it is stored.

[0036] In the figure:

[0037] 1. ELISA instrument body; 101. first level; 102. second level;

[0038] 2. Support frame; 201. T-slot; 202. Slide slot; 203. Through hole; 204. Suction cup; 205. Assembly hole;

[0039] 3. Support plate; 301. Mounting plate; 302. Hemispherical block; 303. Angle plate; 304. First mounting groove; 305. Second mounting groove; 306. Positioning hole;

[0040] 4. Gravity guide assembly; 401. Ball; 402. First connecting rod; 403. Second connecting rod; 404. High-density weighted ball; 405. Assembly plate;

[0041] 5. Positioning assembly; 501. Positioning block; 502. Threaded rod; 503. Rubber pad; 504. Cross slot; 505. Cross plate; 506. Connecting plate; 507. Rotating shaft; 508. Knob; 509. First spring;

[0042] 6. Pin top assembly; 601. Insert plate; 602. Linkage plate; 603. L-shaped plate; 604. Slide plate; 605. Synchronous plate; 606. Second spring; 607. Protective cover;

[0043] 7. Protection assembly; 701. Guard plate; 702. Soft protection pad; 703. Guide rod; 704. Sleeve; 705. Third spring; 706. Piston. DETAILED DESCRIPTION

[0044] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the accompanying drawings in the embodiments of the present invention.

[0045] See also Figure 1-8 , the embodiment of the present invention provides the following two technical solutions:

[0046] Embodiment 1: An electrochemical detector with a horizontal calibration effect comprises an ELISA instrument body 1 , a support frame 2 , a support plate 3 , a gravity guide assembly 4 , a positioning assembly 5 and two pin top assemblies 6 .

[0047] As a preferred solution, in order to facilitate the horizontal calibration of the microplate reader body 1, the front and one side of the microplate reader body 1 are respectively embedded and fixed with a first level 101 and a second level 102, and the axes of the first level 101 and the second level 102 are vertically crossed. When the first level 101 and the second level 102 are both in a horizontal state, it can be judged that the microplate reader body 1 is in a horizontal state.

[0048] As a preferred solution, the support plate 3 includes two mounting plates 301, and the opposite sides of the two mounting plates 301 are rotatably connected to the front and rear sides of the inner cavity of the support frame 2 through pins, and a hemispherical block 302 is fixedly installed on one side of the top of the mounting plate 301. The two hemispherical blocks 302 are used in conjunction with each other, and the two hemispherical blocks 302 are fixedly connected by an angle plate 303. The gravity guide component 4 includes a ball 401, and the top of the ball 401 is fixedly connected to an assembly plate 405 through a first connecting rod 402. The assembly plate 405 is fixedly installed on the bottom of the microplate reader body 1, and the bottom of the ball 401 is fixedly installed with a high-density matching through a second connecting rod 403. The heavy ball 404, the two hemispherical blocks 302 have opposite sides each provided with a first mounting groove 304 and two second mounting grooves 305, the first mounting groove 304 is used in conjunction with the ball 401, the second mounting groove 305 is used in conjunction with the first connecting rod 402 and the second connecting rod 403 respectively, and the first mounting groove 304 is connected to the two second mounting grooves 305 respectively. It is further explained that in order to ensure that the high-density counterweight ball 404 can be calibrated for the automated level of the ELISA instrument body 1 under gravity traction, the weight of the high-density counterweight ball 404 is 0.5-3 times the weight of the ELISA instrument body 1, and the high-density counterweight ball 404 is preferably a solid stainless steel ball.

[0049] As a preferred solution, in order to ensure the stability of the ELISA instrument body 1 after horizontal calibration, the positioning assembly 5 includes a positioning block 501, one side of the positioning block 501 is penetrated and threadedly installed with a threaded rod 502, one end of the threaded rod 502 is fixedly installed with a rubber pad 503, and the outer arc surfaces of the two hemispherical blocks 302 are each provided with a positioning hole 306, the positioning hole 306 is connected to the first mounting groove 304, and the inner surface of the positioning hole 306 is used in conjunction with the outer periphery of the threaded rod 502 and the rubber pad 503, respectively.

[0050] Further explanation, in order to facilitate the rotational drive of the threaded rod 502, a cross groove 504 is provided at the other end of the threaded rod 502, and a cross plate 505 is slidably installed inside the cross groove 504, one end of the cross plate 505 is fixedly connected to a connecting disk 506, one side of the connecting disk 506 is fixedly connected to a rotating shaft 507, one end of the rotating shaft 507 is fixedly connected to a knob 508, and a first spring 509 is sleeved on the outer periphery of the threaded rod 502, and the two ends of the first spring 509 are respectively fixedly connected to the side of the connecting disk 506 opposite to the positioning block 501.

[0051] As a preferred solution, in order to ensure the stability of the support plate 3 and when the ELISA instrument body 1 is not in use, the high-density counterweight ball 404 can be used as a handle to carry the entire device for convenient movement. The pin top assembly 6 includes two plug plates 601. The top of the plug plate 601 is slidably matched with the bottom of the mounting plate 301. The bottom of the two plug plates 601 is rotatably installed with a linkage plate 602 through a rotating block. The bottom of the linkage plate 602 is rotatably connected to an L-shaped plate 603 through a rotating block. The bottom of one side of the L-shaped plate 603 is fixedly installed with a slide plate 604. Two T-slots 201 and two slide grooves 202 are provided on the front and rear sides of the support frame 2. The two T-slots 201 are used in conjunction with two plug plates 601 and a linkage plate 602 respectively. The two slide grooves 202 are used in conjunction with two slide plates 604 respectively. A synchronization plate 605 is fixedly installed at the bottom of the two slide plates 604. The synchronization plate 605 is arranged inside the support frame 2, and a plurality of second springs 606 are fixedly installed at the bottom of the synchronization plate 605. The bottom ends of the second springs 606 are fixedly connected to the bottom of the inner cavity of the support frame 2.

[0052] Furthermore, protective covers 607 are fixedly installed on both the front and rear sides of the support frame 2. The protective covers 607 are arranged on the top peripheries of the two plug plates 601, the linkage plate 602 and the L-shaped plate 603. The bottom of the L-shaped plate 603 passes through the protective cover 607 and extends to the bottom of the protective cover 607.

[0053] In this embodiment, the microplate reader body 1 can be automatically calibrated horizontally to adapt to work surfaces with different inclinations, and the microplate reader body 1 can be transferred to the inside of the support frame 2 by rotation, using the high-density counterweight ball 404 as a handle to facilitate carrying of the entire device.

[0054] Embodiment 2. This embodiment is an improvement on the previous embodiment. It is an electrochemical detector with a horizontal calibration effect. A protective component 7 is provided at the bottom of the inner cavity of the support frame 2. The protective component 7 includes a protective plate 701. A soft protective pad 702 is fixedly installed on the top of the protective plate 701. A plurality of guide rods 703 are fixedly installed on the left and right sides of the bottom of the protective plate 701. A sleeve 704 is sleeved and slidably installed on the outer periphery of the guide rod 703. The sleeve 704 is fixedly installed on the bottom of the inner cavity of the support frame 2. The slide plate 604 is arranged between the protective plate 701 and the sleeve 704. A third spring 705 is sleeved on the outer periphery of the sleeve 704 and the guide rod 703. The two ends of the third spring 705 are fixedly connected to the bottom of the protective plate 701 and the bottom of the inner cavity of the support frame 2, respectively. In this way, when the ELISA instrument body 1 moves to the inside of the support frame 2, the touch screen of the ELISA instrument body 1 can be covered and protected by the soft protective pad 702.

[0055] As a preferred solution, a plurality of through holes 203 connected to the sleeve 704 are provided at the bottom of the support frame 2. A suction cup 204 is fixedly installed at the bottom of the support frame 2 and at the periphery of the through hole 203, and a piston 706 used in conjunction with the sleeve 704 is fixedly connected to the bottom end of the guide rod 703. When the device is placed on a work surface and the ELISA instrument body 1 needs to be used, it is necessary to first press down the guard plate 701 to separate the soft protective pad 702 from the ELISA instrument body 1 before the ELISA instrument body 1 can be rotated to the top of the support frame 2. At this time, when the suction cup 204 is in contact with the work surface, with the rebound of the third spring 705, the suction cup 204 can be stably adsorbed on the work surface, effectively ensuring the overall stability of the device, and when the ELISA instrument body 1 is rotated into the support frame 2, it is also necessary to press down the guard plate 701, so that the guide rod 703 will push the piston 706 to squeeze the air inside the sleeve 704, facilitating the separation of the suction cup 204 from the work surface.

[0056] As a preferred solution, in order to facilitate the batch placement and transportation of the device when not in use, the bottom of the support frame 2 is also provided with an assembly hole 205 for use with the high-density counterweight ball 404.

[0057] The advantage of the second embodiment over the first embodiment is that, while ensuring the contact stability between the device and the work surface, the touch screen of the microplate reader body 1 can be covered and protected when the microplate reader body 1 is not in use.

[0058] A method for using an electrochemical detector with a level calibration effect specifically comprises the following steps:

[0059] Step 1: The current device is as follows Figure 8 In the state shown, the guard plate 701 is squeezed downward, so that the guard plate 701 drives the soft protective pad 702 away from the ELISA instrument body 1, the guard plate 701 squeezes the third spring 705, and the guide rod 703 is continuously inserted into the sleeve 704. During the process, the guard plate 701 contacts the slide plate 604 and pushes the slide plate 604 to slide downward in the slide groove 202. The slide plate 604 drives the synchronous plate 605 to squeeze the second spring 606 downward. At the same time, the slide plate 604 drives the L-shaped plate 603 to move downward, and the L-shaped plate 603 drives the linkage plate 602 to move the plug plate 601 into the protective cover 607. After the plug plate 601 is separated from the mounting plate 301, the high-density counterweight ball 404 is pulled to rotate the mounting plate 301 180 degrees, so that the high-density counterweight ball 404 enters the support frame 2. At this time, it appears as shown in the attached figure. Figure 1 Status shown;

[0060] Step 2: Place the support frame 2 on the work surface, the suction cup 204 contacts the work surface, release the squeeze on the guard plate 701, the third spring 705 pushes the guard plate 701 to reset, the second spring 606 pushes the synchronization plate 605 to squeeze the slide plate 604 to rise, so that the suction cup 204 is adsorbed on the work surface, and at the same time the slide plate 604 pushes the linkage plate 602 to move the plug plate 601 to the bottom of the mounting plate 301;

[0061] Step 3, when the microplate reader body 1 is no longer shaking, hold the microplate reader body 1, keep the first level meter 101 and the second level meter 102 in a horizontal state, pull the knob 508, the knob 508 drives the connecting disk 506 through the rotating shaft 507 to extend the first spring 509, and drives the cross plate 505 to slide in the cross groove 504 through the connecting disk 506, turn the knob 508, the knob 508 drives the connecting disk 506 through the rotating shaft 507 to rotate the cross plate 505, the cross plate 505 drives the threaded rod 502 to rotate, the threaded rod 502 pushes the rubber pad 503 into the positioning hole 306, squeezes and fixes the ball 401, and then releases the knob 508, the first spring 509 pulls the connecting disk 506 to reset the knob 508, and the horizontal calibration positioning of the microplate reader body 1 is completed;

[0062] Step 4: After the use of the microplate reader body 1 is completed, the knob 508 is rotated in the reverse direction to separate the rubber pad 503 from the ball 401, and the guard plate 701 is squeezed downward. The guard plate 701 squeezes the third spring 705, and the guide rod 703 is continuously inserted into the sleeve 704 to reduce the suction force between the suction cup 204 and the work surface. During the process, the guard plate 701 contacts the slide plate 604 and pushes the slide plate 604 to slide downward in the slide groove 202. The slide plate 604 drives the synchronous plate 605 to squeeze the second spring 606 downward. At the same time, the slide plate 604 drives the L-shaped plate 603 to move downward, and the L-shaped plate 603 drives the linkage plate 602 makes the plug plate 601 move to the inside of the protective cover 607. After the plug plate 601 is separated from the mounting plate 301, the high-density counterweight ball 404 is pulled to rotate the mounting plate 301 180 degrees, so that the high-density counterweight ball 404 moves to the top of the support frame 2, and the extrusion on the guard plate 701 is released. The third spring 705 pushes the guard plate 701 to reset, and the second spring 606 pushes the synchronization plate 605 to squeeze the slide plate 604 to rise. The slide plate 604 pushes the linkage plate 602 to move the plug plate 601 to the bottom of the mounting plate 301. The display screen of the microplate reader body 1 contacts the soft protective pad 702. At this time, the device as a whole appears as shown in the attached figure. Figure 8 Status shown.

[0063] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An electrochemical detector with a horizontal calibration effect, comprising an ELISA body (1) and a support frame (2) arranged below the ELISA body (1), characterized in that: A support plate (3) is rotatably mounted between the tops of the front and rear sides of the inner cavity of the support frame (2), a gravity guide component (4) is rotatably mounted in the support plate (3), the ELISA instrument body (1) is fixedly mounted on the top of the gravity guide component (4), a positioning component (5) is arranged on the top of the support plate (3), and the positioning component (5) is used in conjunction with the gravity guide component (4); The front and rear sides of the support frame (2) are both provided with pin top assemblies (6), and the pin top assemblies (6) are used in conjunction with the support plate (3); A protective component (7) is provided at the bottom of the inner cavity of the support frame (2), and the protective component (7) is used in conjunction with the pin top component (6) and the ELISA instrument body (1).

2. The electrochemical detector with level calibration effect according to claim 1, characterized in that: The support plate member (3) comprises two mounting plates (301), and a hemispherical block (302) is fixedly mounted on one side of the top of each mounting plate (301). The two hemispherical blocks (302) are used in conjunction with each other, and the two hemispherical blocks (302) are fixedly connected via an angle plate (303); The opposite sides of the two mounting plates (301) are rotatably connected to the front and rear sides of the inner cavity of the support frame (2) via pins.

3. The electrochemical detector with level calibration effect according to claim 2, characterized in that: The gravity guide assembly (4) comprises a ball (401), the top of the ball (401) is fixedly connected to a mounting plate (405) via a first connecting rod (402), and the bottom of the ball (401) is fixedly mounted with a high-density weighted ball (404) via a second connecting rod (403), wherein the weight of the high-density weighted ball (404) is 0.5-3 times the weight of the microplate reader body (1); A first mounting groove (304) and two second mounting grooves (305) are provided on opposite sides of the two hemispherical blocks (302); the first mounting groove (304) is used in conjunction with the ball (401); the second mounting groove (305) is used in conjunction with the first connecting rod (402) and the second connecting rod (403), respectively; and the first mounting groove (304) is connected to the two second mounting grooves (305) respectively; The assembly plate (405) is fixedly mounted on the bottom of the microplate reader body (1), and the front and one side of the microplate reader body (1) are respectively inlaid with a first level (101) and a second level (102), and the axes of the first level (101) and the second level (102) are vertically intersected.

4. The electrochemical detector with level calibration effect according to claim 3, characterized in that: The positioning assembly (5) comprises a positioning block (501), a threaded rod (502) passing through and threadedly mounted on one side of the positioning block (501), and a rubber pad (503) fixedly mounted on one end of the threaded rod (502); The outer arc surfaces of the two hemispherical blocks (302) are each provided with a positioning hole (306), the positioning hole (306) is connected to the first mounting groove (304), and the inner surface of the positioning hole (306) is respectively used in conjunction with the outer periphery of the threaded rod (502) and the rubber pad (503).

5. The electrochemical detector with level calibration effect according to claim 4, characterized in that: The other end of the threaded rod (502) is provided with a cross groove (504), a cross plate (505) is slidably mounted inside the cross groove (504), one end of the cross plate (505) is fixedly connected to a connecting disk (506), one side of the connecting disk (506) is fixedly connected to a rotating shaft (507), one end of the rotating shaft (507) is fixedly connected to a knob (508), the outer periphery of the threaded rod (502) is sleeved with a first spring (509), and the two ends of the first spring (509) are respectively fixedly connected to the side of the connecting disk (506) opposite to the positioning block (501).

6. The electrochemical detector with level calibration effect according to claim 2, characterized in that: The pin top assembly (6) comprises two plug plates (601), the bottom of the two plug plates (601) are rotatably mounted with a linkage plate (602) via a rotating block, the bottom of the linkage plate (602) is rotatably connected with an L-shaped plate (603) via a rotating block, a slide plate (604) is fixedly mounted at the bottom of one side of the L-shaped plate (603), a synchronization plate (605) is fixedly mounted at the bottom of the two slide plates (604), and a plurality of second springs (606) are fixedly mounted at the bottom of the synchronization plate (605).

7. The electrochemical detector with level calibration effect according to claim 6, characterized in that: The support frame (2) is provided with two T-shaped slots (201) and two slide slots (202) on both the front and rear sides. The two T-shaped slots (201) are respectively used in conjunction with the two plug plates (601) and the linkage plate (602). The two slide slots (202) are respectively used in conjunction with the two slide plates (604). The top of the plug plate (601) is slidably matched with the bottom of the mounting plate (301). The synchronization plate (605) is arranged inside the support frame (2), and the bottom end of the second spring (606) is fixedly connected to the bottom of the inner cavity of the support frame (2). Protective covers (607) are fixedly mounted on both the front and rear sides of the support frame (2); the protective covers (607) are arranged on the top peripheries of the two plug plates (601), the linkage plate (602) and the L-shaped plate (603); the bottom of the L-shaped plate (603) passes through the protective cover (607) and extends to the bottom of the protective cover (607).

8. The electrochemical detector with level calibration effect according to claim 7, characterized in that: The protection assembly (7) comprises a protection plate (701), a soft protection pad (702) is fixedly mounted on the top of the protection plate (701), a plurality of guide rods (703) are fixedly mounted on both left and right sides of the bottom of the protection plate (701), and a sleeve (704) is sleeved and slidably mounted on the outer periphery of the guide rod (703); The sleeve (704) is fixedly mounted on the bottom of the inner cavity of the support frame (2); a third spring (705) is sleeved on the outer circumference of the sleeve (704) and the guide rod (703); and two ends of the third spring (705) are respectively fixedly connected to the bottom of the guard plate (701) and the bottom of the inner cavity of the support frame (2); The slide plate (604) is arranged between the guard plate (701) and the sleeve (704).

9. The electrochemical detector with level calibration effect according to claim 8, characterized in that: The bottom of the support frame (2) is provided with a plurality of through holes (203) in communication with the sleeve (704); a suction cup (204) is fixedly installed at the bottom of the support frame (2) and on the outer periphery of the through holes (203); and a piston (706) used in conjunction with the sleeve (704) is fixedly connected to the bottom end of the guide rod (703); The bottom of the support frame (2) is also provided with an assembly hole (205) for use with a high-density counterweight ball (404).

10. A method for using an electrochemical detector having a level calibration effect, characterized in that: The specific steps include: Step 1: Press the guard plate (701) downward, so that the guard plate (701) drives the soft protective pad (702) away from the ELISA instrument body (1), the guard plate (701) presses the third spring (705), and the guide rod (703) is continuously inserted into the sleeve (704). During the process, the guard plate (701) contacts the slide plate (604) and pushes the slide plate (604) to slide downward in the slide groove (202), and the slide plate (604) drives the synchronous plate (60 5) The second spring (606) is pressed downward, and at the same time, the slide plate (604) drives the L-shaped plate (603) to move downward, and the L-shaped plate (603) drives the linkage plate (602) to move the plug plate (601) toward the inside of the protective cover (607). After the plug plate (601) is separated from the mounting plate (301), the high-density counterweight ball (404) is pulled to rotate the mounting plate (301) 180 degrees, so that the high-density counterweight ball (404) enters the inside of the support frame (2); Step 2: Place the support frame (2) on the work surface, release the pressure on the guard plate (701), the third spring (705) pushes the guard plate (701) to reset, the second spring (606) pushes the synchronization plate (605) to squeeze the slide plate (604) to rise, and the slide plate (604) pushes the linkage plate (602) to move the plug plate (601) to the bottom of the installation plate (301); Step 3: When the ELISA instrument body (1) is no longer shaking, hold the ELISA instrument body (1), keep the first level meter (101) and the second level meter (102) in a horizontal state, pull the knob (508), the knob (508) drives the connecting plate (506) through the rotating shaft (507) to extend the first spring (509), and drives the cross plate (505) to slide in the cross groove (504) through the connecting plate (506), and turn the knob (508). ) The connecting plate (506) is driven by the rotating shaft (507) to rotate the cross plate (505), the cross plate (505) drives the threaded rod (502) to rotate, the threaded rod (502) pushes the rubber pad (503) into the positioning hole (306), squeezes and fixes the ball (401), and then releases the knob (508), the first spring (509) pulls the connecting plate (506) to reset the knob (508), and the horizontal calibration positioning of the microplate reader body (1) is completed; Step 4: After the use of the ELISA instrument body (1), the knob (508) is rotated in the reverse direction to separate the rubber pad (503) from the ball (401), and the guard plate (701) is pressed downward. The guard plate (701) presses the third spring (705), and the guide rod (703) is continuously inserted into the sleeve (704). During the process, the guard plate (701) contacts the slide plate (604) and pushes the slide plate (604) to slide downward in the slide groove (202). The slide plate (604) drives the synchronous plate (605) to press the second spring (606) downward. At the same time, the slide plate (604) drives the L-shaped plate (603) to move downward, and the L-shaped plate (603) drives the linkage plate (602) to make the insertion The plate (601) moves toward the inside of the protective cover (607). After the plug plate (601) is separated from the mounting plate (301), the high-density counterweight ball (404) is pulled to rotate the mounting plate (301) 180 degrees, so that the high-density counterweight ball (404) moves to the top of the support frame (2), and the extrusion on the guard plate (701) is released. The third spring (705) pushes the guard plate (701) to reset, and the second spring (606) pushes the synchronization plate (605) to squeeze the slide plate (604) to rise. The slide plate (604) pushes the linkage plate (602) to move the plug plate (601) to the bottom of the mounting plate (301), and the display screen of the ELISA instrument body (1) contacts the soft protective pad (702).

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