Composition for reducing active oxygen

Abstract

The purpose of the present invention is to provide a composition for reducing active oxygen, which is capable of suppressing a decrease in photosynthetic ability due to ROS. One embodiment of the present invention is a composition for reducing active oxygen, comprising: at least one biosurfactant selected from the group consisting of a peptide-type biosurfactant and a sugar-type biosurfactant; and an agriculturally acceptable carrier.

Description

Technical Field

[0001] The present disclosure relates to a composition for reducing active oxygen. Background Art

[0002] In order to make plants (vegetables, fruit trees, etc.) grow efficiently, it is known that there are pharmaceutical compositions for imparting environmental stress tolerance to plants (for example, refer to Patent Document 1). Patent Document 1 discloses a pharmaceutical composition for imparting environmental stress tolerance to plants containing a yeast cell wall enzymatic decomposition product. Patent Document 1 discloses that by applying a yeast cell wall enzymatic decomposition product to seedlings and / or seedlings after being planted in the field, tolerance to environmental stresses such as temperature stress, salt stress, light stress, and water stress can be improved, and a surfactant can be combined, and brewer's yeast is used in the examples.

[0003] Patent document 2 discloses a soil treatment composition for improving the immune health, growth and / or yield of plants, which contains Wickerhamomyces anomalus yeast (abnormal Wickerham yeast) and / or its growth byproducts. Patent document 2 discloses that the soil treatment composition is a microbial-based soil treatment composition, which is used to improve the health and / or growth of the root system of the plant and stimulate the natural immunity and other metabolic systems of the plant that contribute to the health and productivity of the plant, thereby improving the health, growth and total yield of crop plants. It should be noted that Wickerhamomyces anomalus yeast is known to be a yeast-like fungus that produces ethyl acetate, so when attached to food, it will cause the smell of thin materials.

[0004] In addition, Patent Document 3 discloses the use of at least one lipopeptide as a plant activator for plant growth. In the examples of Patent Document 3, the photosynthetic efficiency at the beginning and end of water stress was evaluated by Φ value (PSII), but no decrease in photosynthetic efficiency was observed when the water stress was compared at the beginning and end.

[0005] Prior art literature

[0006] Patent Literature

[0007] Patent Document 1: Japanese Patent Application Publication No. 2007-45709

[0008] Patent Document 2: Japanese Patent Application No. 2022-509204

[0009] Patent Document 3: Japanese Patent Application No. 2020-504768 Summary of the invention

[0010] Problems to be solved by the invention

[0011] It is known that when plants (vegetables, fruit trees, etc.) are exposed to abiotic stresses caused by high temperature, low temperature, salt, dryness, etc., the CO2 supply from the stomata decreases, and the reducing power obtained from light energy cannot be fully consumed, resulting in the generation of ROS (Reactive Oxygen Species), and the photosynthetic capacity will be reduced due to ROS.

[0012] However, Patent Documents 1 to 3 do not describe or teach a decrease in photosynthetic capacity due to ROS, nor do they describe or teach a composition capable of suppressing a decrease in photosynthetic capacity due to ROS.

[0013] That is, an object of the present disclosure is to provide a composition for reducing active oxygen species that can suppress a decrease in photosynthetic capacity caused by ROS.

[0014] Solution to the problem

[0015] The form of this embodiment is described below, for example.

[0016] [1] A composition for reducing active oxygen species, comprising: at least one biosurfactant selected from a peptide-type biosurfactant and a sugar-type biosurfactant; and an agriculturally acceptable carrier.

[0017] [2] The composition for reducing active oxygen species according to [1], wherein the biosurfactant is contained in an amount of 0.004 to 0.2% by mass based on 100% by mass of the composition for reducing active oxygen species.

[0018] [3] The composition for reducing active oxygen species according to [1] or [2], comprising a phospholipid.

[0019] [4] The composition for reducing active oxygen according to [3], wherein 0.004 to 0.2 mass % of the biosurfactant is contained in 100 mass % of the composition for reducing active oxygen,

[0020] The above-mentioned active oxygen reducing composition contains 0.002 to 0.1% by mass of the above-mentioned phospholipid in 100% by mass.

[0021] [5] The composition for reducing active oxygen according to any one of [1] to [4], comprising a soybean meal decomposition product.

[0022] [6] The composition for reducing active oxygen according to [5], wherein 0.004 to 0.2 mass % of the biosurfactant is contained in 100 mass % of the composition for reducing active oxygen,

[0023] The above-mentioned active oxygen reducing composition contains 0.004 to 1.0% by mass of the above-mentioned soybean meal decomposition product in 100% by mass.

[0024] [7] The composition for reducing active oxygen according to any one of [1] to [6], wherein the biosurfactant is at least one biosurfactant selected from surfactin, rhamnolipid, sophorolipid, and salts thereof.

[0025] [8] The composition for reducing active oxygen species according to any one of [1] to [7], which is used for spreading on leaves of plants.

[0026] [9] A precursor of a composition for reducing active oxygen species, which, upon dilution, becomes the composition for reducing active oxygen species according to any one of [1] to [8].

[0027] This specification includes the disclosure of Japanese Patent Application No. 2022-158110 which is the basis of priority of this application.

[0028] Effects of the Invention

[0029] The composition for reducing active oxygen species disclosed herein can sufficiently suppress the generation of ROS. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 The ETR of Experimental Example 1 is shown.

[0031] Figure 2 The SPAD value of Experimental Example 1 is shown.

[0032] Figure 3 The ETR of Experimental Example 2 is shown.

[0033] Figure 4 The SPAD value of Experimental Example 2 is shown.

[0034] Figure 5 The ETR of Experimental Example 3 is shown.

[0035] Figure 6 The SPAD value of Experimental Example 3 is shown.

[0036] Figure 7 The ETR of Experimental Example 4 is shown.

[0037] Figure 8 The SPAD value of Experimental Example 4 is shown.

[0038] Fig. 9 The ETR of Experimental Example 5 is shown.

[0039] Fig.10 The SPAD value of Experimental Example 5 is shown.

[0040] Fig.11 The ETR of Experimental Example 6 is shown.

[0041] Fig.12 The SPAD value of Experimental Example 6 is shown.

[0042] Fig.13 The ETR of Experimental Example 7 is shown.

[0043] Fig.14 The SPAD value of Experimental Example 7 is shown.

[0044] Fig.15 The ETR of Experimental Example 8 is shown.

[0045] Fig.16 The SPAD value of Experimental Example 8 is shown.

[0046] Fig.17 The ETR of Experimental Example 9 is shown.

[0047] Fig.18 The SPAD value of Experimental Example 9 is shown. DETAILED DESCRIPTION

[0048] Hereinafter, the present invention will be described in detail.

[0049] The active oxygen reduction composition of the present embodiment comprises: at least one biosurfactant selected from a peptide biosurfactant and a sugar biosurfactant, and an agriculturally acceptable carrier. It should be noted that the active oxygen reduction composition of the present embodiment can not only inhibit the generation of active oxygen, but also inhibit the generation of ROS, and therefore, the active oxygen reduction composition can also be expressed as a ROS reduction composition.

[0050] <Biosurfactant>

[0051] The above-mentioned composition for reducing active oxygen species contains a biosurfactant. As the biosurfactant, at least one biosurfactant selected from a peptide-type biosurfactant and a sugar-type biosurfactant can be cited. The above-mentioned composition for reducing active oxygen species can contain one biosurfactant alone, or it can contain two or more. Since the above-mentioned composition for reducing active oxygen species contains a biosurfactant, it is possible to inhibit the production of ROS by applying it to plants. The reason is not clear, and this effect is not observed even when a synthetic surfactant is used. This is an effect unique to biosurfactants. It should be noted that as a biosurfactant, from the viewpoint of odor and food hygiene, it is preferred to use a biosurfactant that is not derived from Wickerhamomyces anomalus yeast.

[0052] As peptide biosurfactants, lipopeptide biosurfactants can be cited. Lipopeptide biosurfactants have peptides containing a hydrophobic group and a hydrophilic part, show surface activity, and are produced by microorganisms. As lipopeptide biosurfactants, for example, surfactin, arthrofactin, iturin, fengycin, serrawettin, lichenysin, viscosin, and salts thereof can be cited.

[0053] As the peptide biosurfactant, at least one peptide biosurfactant selected from surfactin and its salts is preferred. Surfactin and its salts can be represented by the following general formula (1). As surfactin and its salts, one or more surfactin can be used.

[0054] [Chemical formula 1]

[0055]

[0056] [In formula (1), X represents an amino acid residue selected from leucine, isoleucine and valine, R represents an alkyl group having 9 to 18 carbon atoms, and M ＋ Each independently represents a hydrogen ion (H + ), alkali metal ions, ammonium ions or pyridine ion.]

[0057] It should be noted that M ＋ In the case of hydrogen ions, CO2 ‐ (M ＋ ) is a carboxyl group (COOH group). ＋ When the hydrogen ion is present, it refers to surfactin, at least one M ＋ Alkali metal ion, ammonium ion or pyridine In the case of ions, it refers to a salt of surfactin. The general formula of surfactin is represented by the following general formula (1').

[0058] [Chemical formula 2]

[0059]

[0060] [In formula (1'), X and R have the same meanings as in formula (1)]

[0061] X is an amino acid residue selected from the group consisting of leucine, isoleucine and valine, and may be an L-form amino acid residue or a D-form amino acid residue, and is preferably an L-form amino acid residue.

[0062] R is an alkyl group having 9 to 18 carbon atoms, and is a linear or branched monovalent saturated hydrocarbon group having 9 to 18 carbon atoms. Examples of the alkyl group having 9 to 18 carbon atoms include n-nonyl, 6-methyloctyl, 7-methyloctyl, n-decyl, 8-methylnonyl, n-undecyl, 9-methyldecyl, n-dodecyl, 10-methylundecyl, n-tridecyl, 11-methyldodecyl, n-tetradecyl, n-pentadecyl, n-hexadecyl, n-heptadecyl, and n-octadecyl, and 10-methylundecyl is preferred.

[0063] M ＋ Each independently represents a hydrogen ion (H + ), alkali metal ions, ammonium ions or pyridine Ions. Alkali metal ions are not particularly limited, and include lithium ions, sodium ions, potassium ions, and the like. Ammonium ions are not particularly limited, and examples thereof include N(R 1 )4 + Represents ammonium ion. 1 Each independently represents hydrogen or an organic group. As the ammonium ion, one preferred embodiment is R 1 Quaternary ammonium ions are all organic groups. Examples of organic groups include alkyl groups, aralkyl groups, and aryl groups. Specifically, examples of alkyl groups include alkyl groups having 1 to 10 carbon atoms, such as methyl, ethyl, n-propyl, isopropyl, n-butyl, and tert-butyl groups. Examples of aralkyl groups include aralkyl groups having 7 to 12 carbon atoms, such as benzyl, methylbenzyl, and phenylethyl groups. Examples of aryl groups include aryl groups having 6 to 15 carbon atoms, such as phenyl, tolyl, and xylyl groups. Examples of ammonium ions include tetramethylammonium ions and tetraethylammonium ions. Examples of pyridine Ions are not particularly limited. ions, and the hydrogen atoms bonded to the carbon atoms constituting the pyridine ring may be substituted with organic groups. ions, as long as they are combined with the N ＋ The bonded substance may be, for example, hydrogen or an organic group. The organic group possessed by the ion can be appropriately used as R 1 The organic groups listed in the description are:

[0064] The two M in the general formula (1) ＋ The two M in the general formula (1) may be the same as or different from each other. ＋ One of the preferred methods is, for example, a portion of M ＋ For hydrogen ions, part of M ＋ is an alkali metal ion. The alkali metal ion is not particularly limited, and represents lithium ion, sodium ion, potassium ion, etc. It should be noted that the two M in the general formula (1)＋ When there are two or more ions, if we focus on a certain molecule (salt), the two M ＋ It can be the same type of ions. ＋ In the case of two ions, the ratio (molar ratio) of one ion A to one ion B is, for example, 1:10 to 10:1, preferably 1:5 to 5:1, and more preferably 1:3 to 3:1. One preferred embodiment is that the two M in the general formula (1) ＋ Part of it is hydrogen ions, and part of it is sodium ions (Na ＋ ).

[0065] Peptide-type biosurfactants such as surfactin or its salt can be obtained by culturing microorganisms, such as strains belonging to Bacillus subtilis, according to known methods, and isolating them from their culture solution. Purified products can be used, or unpurified products, such as culture solution, can be used directly. In addition, if the molecular structure is the same, products obtained by chemical synthesis can also be used in the same way. In addition, commercially available products can also be used.

[0066] Examples of sugar-type biosurfactants include rhamnolipids, sophorolipids, mannosylerythritol lipids, cellobiose lipids, trehalose lipids, succinoyl trehalose lipids, glucose lipids, polyol lipids, oligosaccharide fatty acid esters, and salts thereof.

[0067] As the sugar-type biosurfactant, at least one sugar-type biosurfactant selected from rhamnolipid, sophorolipid, and salts thereof is preferred.

[0068] The sugar-type biosurfactant can be obtained by a known method, or a commercially available product can be used.

[0069] As the biosurfactant, at least one biosurfactant selected from the group consisting of surfactin, rhamnolipid, sophorolipid, and salts thereof is particularly preferred.

[0070] <Phospholipids>

[0071] The above-mentioned composition for reducing active oxygen species may contain phospholipids. When the above-mentioned composition for reducing active oxygen species contains phospholipids, the ROS reducing effect can be exhibited even when the concentration of the biosurfactant is low, which is preferable.

[0072] As phospholipids, for example, lecithin can be cited. Lecithin is another name for phosphatidylcholine, and is roughly divided into natural lecithin and synthetic lecithin. Natural lecithin refers to a phospholipid of natural origin that contains a large amount of phosphatidylcholine. For example, lecithin derived from egg yolk is called "egg yolk lecithin", and lecithin derived from soybean is called "soybean lecithin". On the other hand, as synthetic lecithin, dimyristoylphosphatidylcholine (DMPC), dipalmitoylphosphatidylcholine (DPPC) and the like are known. In this specification, "lecithin" includes any lecithin in natural lecithin and synthetic lecithin.

[0073] Examples of the phospholipids other than phosphatidylcholine include glycerophospholipids such as phosphatidic acid, diphosphatidic acid, phosphatidylserine, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylglycerol, and diphosphatidylglycerol; sphingophospholipids such as sphingosine, ceramide, sphingomyelin, and cerebroside; and hydrogenated phosphatidylcholines such as hydrogenated soybean lecithin and hydrogenated egg yolk lecithin.

[0074] The phospholipid is preferably one or more phospholipids selected from phosphatidylcholine, hydrogenated phosphatidylcholine, phosphatidic acid, diphosphatidic acid, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylserine, phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol, and sphingomyelin.

[0075] <Soybean meal decomposition products>

[0076] The active oxygen reduction composition may contain a soybean meal decomposition product. When the active oxygen reduction composition contains a soybean meal decomposition product, the ROS reduction effect can be exerted even when the concentration of the biosurfactant is low, which is preferred.

[0077] The soybean meal degradation product can be obtained by, for example, subjecting soybean meal to a Bacillus fermentation treatment using bacteria belonging to the genus Bacillus.

[0078] <Carriers permitted in agriculture>

[0079] The above-mentioned active oxygen reduction composition contains an agriculturally acceptable carrier. In the present disclosure, as an agriculturally acceptable carrier, as long as it is a carrier that can hold the above-mentioned biosurfactant, it can be a liquid carrier or a solid carrier. As a solid carrier, a hydratable solid can be cited. The solid carrier can be in the form of a powder or granules.

[0080] The carrier permitted in agriculture is preferably a liquid carrier such as water or an organic solvent. Here, the water as the carrier is not limited to pure water, and may be an aqueous solution, an aqueous suspension, an aqueous gel or an aqueous slurry, and may have viscosity. Similarly, the organic solvent is not limited to a pure organic solvent, and may be a solution, a suspension, a gel or a slurry based on an organic solvent, and may have viscosity. Examples of the organic solvent include methyl ether, ethyl ether, propyl ether and butyl ether.

[0081] The carrier permitted in agriculture is preferably a liquid carrier such as water, an aqueous solution in which a hydrating substance is dissolved in water, or a solid carrier comprising a hydrating substance soluble in water. As the hydrating substance, polyvinyl pyrrolidone, random and block polymers of oxyalkylene, vinyl acetate / vinyl pyrrolidone copolymers, alkylated vinyl pyrrolidone copolymers, polyalkylene glycols comprising polypropylene glycol and polyethylene glycol, polyvinyl acetate, polyvinyl alcohol, gelatin, agar, gum arabic, karaya gum, tragacanth gum, guar gum, locust bean gum, xanthan gum, ghatti gum, carrageenan, alginate, casein, dextran, pectin, chitin, 2-hydroxyethyl starch, 2-aminoethyl starch, 2-hydroxyethyl cellulose, methyl cellulose, carboxymethyl cellulose salts, cellulose sulfate, polyacrylic acid amide, alkali metal salts of maleic anhydride copolymers, alkali metal salts of poly (meth) acrylates, etc. can be exemplified.

[0082] <Additives>

[0083] The above-mentioned active oxygen reduction composition may contain ingredients other than the above-mentioned biosurfactant, phospholipid, soybean meal decomposition product and agriculturally permitted carrier as additives as needed. The above-mentioned active oxygen reduction composition may contain one additive or two or more additives. As additives, there may be mentioned: moisturizers, colorants, defoamers, UV protectants, antifreeze agents, preservatives, bio-inhibitors or biocides, emulsifiers, extenders, scavengers, plasticizers, flow agents, melting aids, waxes, and / or fillers (e.g., clay, talc, glass fiber, cellulose, micronized wood, etc.), but are not limited thereto.

[0084] <Active oxygen reducing composition>

[0085] As described above, the composition for reducing active oxygen species includes at least one biosurfactant selected from the group consisting of peptide biosurfactants and sugar biosurfactants, and an agriculturally acceptable carrier.

[0086] In the present disclosure, “substantially free of” a certain component means that the amount of the component is 0.01 parts by mass or less, preferably 0.001 parts by mass or less, based on 100 parts by mass of the biosurfactant contained in the active oxygen reducing composition.

[0087] For the above-mentioned active oxygen reduction composition, it is preferred that the above-mentioned biosurfactant is contained in 100% by mass of the above-mentioned active oxygen reduction composition by 0.004 to 0.2% by mass, and more preferably by 0.005 to 0.1% by mass. In addition, when the above-mentioned active oxygen reduction composition does not substantially contain phospholipids and soybean meal decomposition products, it is preferred that the above-mentioned biosurfactant is contained in 100% by mass of the above-mentioned active oxygen reduction composition by 0.01 to 0.2% by mass, and more preferably by 0.02 to 0.1% by mass. When it is within the above range, it is particularly suitable for inhibiting the generation of ROS, so it is preferred.

[0088] One of the preferred modes is that the above-mentioned active oxygen reduction composition includes the above-mentioned biosurfactant and phospholipid. In the case where the above-mentioned active oxygen reduction composition includes the above-mentioned biosurfactant and phospholipid, it is preferred that the above-mentioned active oxygen reduction composition 100% by mass includes the above-mentioned biosurfactant 0.004~0.2% by mass, the above-mentioned active oxygen reduction composition 100% by mass includes the above-mentioned phospholipid 0.002~0.1% by mass, and more preferably the above-mentioned active oxygen reduction composition 100% by mass includes the above-mentioned biosurfactant 0.005~0.1% by mass, and the above-mentioned active oxygen reduction composition 100% by mass includes the above-mentioned phospholipid 0.0025~0.05% by mass. In addition, in the case where the above-mentioned active oxygen reduction composition includes the above-mentioned biosurfactant and phospholipid, it is preferably substantially free of soybean meal decomposition products. When within the above range, it is particularly suitable for inhibiting the generation of ROS, so it is preferred.

[0089] One of the preferred embodiments is that the above-mentioned active oxygen reduction composition includes the above-mentioned biosurfactant and soybean meal decomposition product. When the above-mentioned active oxygen reduction composition includes the above-mentioned biosurfactant and soybean meal decomposition product, it is preferred that the above-mentioned active oxygen reduction composition contains 0.004 to 0.2% by mass of the above-mentioned biosurfactant in 100% by mass, and more preferably contains 0.005 to 0.1% by mass. In addition, when the above-mentioned active oxygen reduction composition includes the above-mentioned biosurfactant and soybean meal decomposition product, it is preferred that the above-mentioned soybean meal decomposition product is contained in 0.004 to 1.0% by mass of the above-mentioned active oxygen reduction composition in 100% by mass, more preferably contains 0.004 to 0.8% by mass, further preferably contains 0.005 to 0.6% by mass, and particularly preferably contains 0.005 to 0.5% by mass. In addition, as another embodiment, when the above-mentioned active oxygen reduction composition includes the above-mentioned biosurfactant and soybean meal decomposition product, it is preferred that the above-mentioned active oxygen reduction composition contains 0.004-0.2 mass% of the above-mentioned biosurfactant in 100 mass%, and the above-mentioned soybean meal decomposition product is 0.004-0.2 mass% in 100 mass% of the above-mentioned active oxygen reduction composition, and more preferably, the above-mentioned biosurfactant is contained in 100 mass% of the above-mentioned active oxygen reduction composition. 0.005-0.1 mass%, and the above-mentioned soybean meal decomposition product is contained in 100 mass% of the above-mentioned active oxygen reduction composition. 0.005-0.1 mass%. In addition, when the above-mentioned active oxygen reduction composition includes the above-mentioned biosurfactant and soybean meal decomposition product, it is preferably substantially free of phospholipids. When it is within the above range, it is particularly suitable for inhibiting the generation of ROS, so it is preferred.

[0090] There are no particular restrictions on the method for producing the above-mentioned active oxygen reduction composition. For example, when the agriculturally acceptable carrier is a liquid carrier, the following method can be mentioned: a method in which a biosurfactant and any additives used, a biosurfactant, a phospholipid and any additives used, or a biosurfactant, a soybean meal decomposition product and any additives used are added to a liquid carrier and stirred until uniform.

[0091] The above-mentioned active oxygen reduction composition is usually applied to plants, for example, it can be used on leaves, seeds, seedlings, fruits, etc. of plants. One of the preferred embodiments is to spread the above-mentioned active oxygen reduction composition on the leaves of plants. It should be noted that spreading on the leaves of plants means at least spreading on the leaves, and can be spread on stems and leaves, and can further be partially spread on flowers, fruits, etc.

[0092] <Precursor of composition for reducing active oxygen>

[0093] As one embodiment of the present disclosure, there can be cited an active oxygen reduction composition precursor that becomes the above-mentioned active oxygen reduction composition by dilution. It should be noted that the dilution is usually carried out by the above-mentioned liquid carrier. The active oxygen reduction composition precursor usually contains at least one biosurfactant selected from peptide biosurfactants and sugar-type biosurfactants, and an agriculturally permitted carrier, and the concentration of the biosurfactant is higher than that of the active oxygen reduction composition. When such an active oxygen reduction composition precursor is used to prepare an active oxygen reduction composition, a uniform active oxygen reduction composition can be easily prepared, so it is preferred.

[0094] It is preferred that the precursor of the composition for reducing active oxygen contains a biosurfactant at a concentration of 10 to 50,000 times that of the composition for reducing active oxygen. In the case where the composition for reducing active oxygen contains a phospholipid, it is preferred that the precursor of the composition for reducing active oxygen contains a phospholipid at a concentration of 10 to 50,000 times that of the composition for reducing active oxygen. In addition, in the case where the composition for reducing active oxygen contains a soybean meal decomposition product, it is preferred that the precursor of the composition for reducing active oxygen contains a soybean meal decomposition product at a concentration of 10 to 50,000 times that of the composition for reducing active oxygen. It should be noted that, in 100% by mass of the precursor of the composition for reducing active oxygen, the biosurfactant contained in the precursor of the composition for reducing active oxygen is preferably 50% by mass or less, and more preferably 20% by mass or less. In addition, in 100% by mass of the precursor of the composition for reducing active oxygen, the agriculturally permitted carrier (preferably a liquid carrier) contained in the precursor of the composition for reducing active oxygen is preferably 20% by mass or more, and more preferably 40% by mass or more.

[0095] <Plant cultivation methods>

[0096] As one embodiment of the present disclosure, there is a method for growing plants by applying the above-mentioned composition for reducing active oxygen to plants. As the method for growing plants, a method of spreading the above-mentioned composition for reducing active oxygen on the leaves of plants is preferred.

[0097] When spraying on the leaves of plants, the composition for reducing active oxygen is preferably sprayed in an amount of preferably 2.5 to 200 g, more preferably 5 to 100 g of the biosurfactant in the composition for reducing active oxygen per 10a (ares) of plant cultivation area. More specifically, when the composition for reducing active oxygen does not substantially contain phospholipids and soybean meal decomposition products, the composition for reducing active oxygen is preferably sprayed in an amount of preferably 20 to 200 g, more preferably 20 to 100 g of the biosurfactant in the composition for reducing active oxygen per 10a of plant cultivation area. When the composition for reducing active oxygen contains a biosurfactant and a phospholipid and does not substantially contain a soybean meal decomposition product, the composition for reducing active oxygen is preferably sprayed in an amount of preferably 4 to 200 g, more preferably 5 to 100 g of the biosurfactant in the composition for reducing active oxygen, and preferably 2 to 100 g, more preferably 2.5 to 50 g of the phospholipid in the composition for reducing active oxygen per 10a of plant cultivation area. In addition, when the above-mentioned active oxygen reduction composition contains a biosurfactant and a soybean meal decomposition product and does not substantially contain phospholipids, it is preferred to spread the above-mentioned active oxygen reduction composition in an amount of preferably 4 to 200 g, more preferably 5 to 100 g of the biosurfactant in the above-mentioned active oxygen reduction composition, and preferably 4 to 1000 g, more preferably 4 to 800 g, further preferably 5 to 600 g, and particularly preferably 5 to 500 g of the soybean meal decomposition product per 10a of plant cultivation area. In addition, as another embodiment, it is preferred to spread the above-mentioned active oxygen reduction composition in an amount of preferably 4 to 200 g, more preferably 5 to 100 g of the soybean meal decomposition product.

[0098] When the active oxygen reducing composition is sprayed on the leaves of plants, the spraying time is not particularly limited, and the composition may be sprayed once or more than once at any time between after germination and before harvesting the plants.

[0099] <Plants>

[0100] In the present disclosure, plants are not particularly limited, and are preferably crop plants. As crop plants, corn (corn, maize), wheat, barley, rye, oats, rice, soybean, rape (Brassica napus), cotton, sunflower, beet, potato, tobacco, broccoli, lettuce, cabbage, spinach, komatsuna, cauliflower, coconut, tomato, cucumber, eggplant, melon, pumpkin, okra, pepper, watermelon, carrot, radish, onion, green onion, fruit trees, flowers, lawn grass, forage grass, etc. can be exemplified.

[0101] Example

[0102] Hereinafter, the present embodiment will be described with reference to Examples, but the present disclosure is not limited to these Examples.

[0103] In the present examples, "SF" refers to surfactant Na (product name: KANEKA Surfactin, manufactured by Kaneka Co., Ltd.), "RL" refers to rhamnolipid (manufactured by AGAE technology), "SL" refers to sophorolipid (prepared according to Journal of Oleo Science, 60, (5) pp.267-273 (2011)), "lecithin" refers to soybean lecithin (manufactured by Nacalai Tesque), KVP refers to soybean meal decomposition product (a peptide raw material produced by subjecting soybean meal to Bacillus fermentation treatment according to APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Jan. 1994, pp. 243-247), "SILWET L-77" refers to a synthetic surfactant manufactured by Momentive, "Tween 20" refers to a synthetic surfactant (manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.), and "Approach "BI" refers to a spreading agent mainly composed of a synthetic surfactant manufactured by Maruwa Biochemical Co., Ltd., and "paraquat" refers to a standard product of paraquat dichloride (manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.).

[0104] It should be noted that in the examples related to Figure 1 to Figure 18 In the figure, the relative value marked in each bar graph is the relative value when T1 in each figure is set to 100. When each bar graph in each figure is marked as X% increase (relative to T2), it means an increase of X% relative to the value of T2 in each figure. When each bar graph in each figure is marked as Y% decrease (relative to T2), it means a decrease of Y% relative to the value of T2 in each figure.

[0105] [Experimental Example 1]

[0106] A 9 cm plastic pot was filled with soil, and three soybeans (Fukuyutaka) were sown.

[0107] After sowing, the seeds were grown in a light period (6:00-18:00, temperature 26°C, illumination 120 μmol / m 2 ·s) and in a dark period (18:00-6:00, temperature 18°C) incubator. Thin out the seedlings to 1 plant after germination.

[0108] After the first true leaf unfolded, the following liquid (active oxygen reduction composition) was sprayed on the leaves of each plant in each treatment area (application of active oxygen reduction composition). 24 hours after the foliar spraying, a solution prepared by adding SILWET L-77 to the paraquat aqueous solution and diluting it 1000 times was sprayed on the leaves of each plant except the control area (T1) (application of paraquat).

[0109] 24 hours after the application of paraquat, the electron transfer rate (ETR) was measured by PAM fluorescence method (pulse amplitude modulated fluorometry). In addition, 24 hours after the application of paraquat, the SPAD value was measured using a chlorophyll meter.

[0110] The active oxygen reducing composition and operation outlines of each treatment zone in Experimental Example 1 are shown in Table 1 below.

[0111]

[0112] Figure 1 The ETR is shown when PAR (Photosynthetically active radiation) is set to 1516. Figure 2 The SPAD values ​​are shown in Figure 1 It is clear that in T2, where water that does not function as a composition for reducing active oxygen was sprayed, the ETR was significantly reduced compared to T1 by applying paraquat. It is known that ROS are generated by the application of paraquat, so it is believed that the difference between T1 and T2 is caused by the application of paraquat. In T3 to T5, where the composition for reducing active oxygen was sprayed, the ETR increased compared to T2, indicating that ROS was reduced in T3 to T5 compared to T2. Figure 2 The SPAD values ​​also show the same result.

[0113] [Experimental Example 2]

[0114] A 9 cm plastic pot was filled with soil, and three soybeans (Fukuyutaka) were sown.

[0115] After sowing, the seeds were grown in a light period (6:00-18:00, temperature 26°C, illumination 120 μmol / m 2 ·s) and in a dark period (18:00-6:00, temperature 18°C) incubator. Thin out the seedlings to 1 plant after germination.

[0116] After the first true leaf unfolded, the following liquid (active oxygen reduction composition) was sprayed on the leaves of each plant in each treatment area (application of active oxygen reduction composition). 24 hours after the foliar spraying, a solution prepared by adding SILWET L-77 to the paraquat aqueous solution and diluting it 1000 times was sprayed on the leaves of each plant except the control area (T1) (application of paraquat).

[0117] ETR was measured by PAM fluorescence method 24 hours after the application of paraquat. In addition, SPAD value was measured using a chlorophyll meter 24 hours after the application of paraquat.

[0118] The active oxygen reduction composition and operation outlines of each treatment zone in Experimental Example 2 are shown in Table 2 below.

[0119]

[0120] Figure 3 The ETR when PAR is set to 1512 is shown in FIG. Figure 4 The SPAD values ​​are shown in Figure 3 It is clear that in T2, ETR was significantly lower than in T1. In T3 to T5 where the active oxygen reduction composition was sprayed, ETR increased compared to T2, indicating that ROS was reduced in T3 to T5 compared to T2. Figure 4 The SPAD values ​​also show the same result.

[0121] [Experimental Example 3]

[0122] A 9 cm plastic pot was filled with soil, and three soybeans (Fukuyutaka) were sown.

[0123] After sowing, the seeds were grown in a light period (6:00-18:00, temperature 26°C, illumination 120 μmol / m 2 ·s) and in a dark period (18:00-6:00, temperature 18°C) incubator. Thin out the seedlings to 1 plant after germination.

[0124] After the first true leaf unfolded, the following liquid (active oxygen reduction composition) was sprayed on the leaves of each plant in each treatment area (application of active oxygen reduction composition). 24 hours after the foliar spraying, a solution prepared by adding SILWET L-77 to the paraquat aqueous solution and diluting it 1000 times was sprayed on the leaves of each plant except the control area (T1) (application of paraquat).

[0125] ETR was measured by PAM fluorescence method 24 hours after the application of paraquat. In addition, SPAD value was measured using a chlorophyll meter 24 hours after the application of paraquat.

[0126] The active oxygen reduction composition and operation outlines of each treatment zone in Experimental Example 3 are shown in Table 3 below.

[0127]

[0128] Figure 5 The ETR when PAR is set to 1496 is shown in FIG. Figure 6 The SPAD values ​​are shown in Figure 5 It is clear that in T2, ETR was significantly lower than in T1. In T3, where a composition for reducing active oxygen with a low concentration of SF was applied, ETR was slightly higher than in T2, and no increase in ETR was observed in T4 without SF. In T5, where a composition for reducing active oxygen containing diluted SF and lecithin was applied, ETR was significantly higher than in T2, indicating that ROS was sufficiently reduced compared to T2.

[0129] [Experimental Example 4]

[0130] A 9 cm plastic pot was filled with soil, and three soybeans (Fukuyutaka) were sown.

[0131] After sowing, the seeds were grown in a light period (6:00-18:00, temperature 26°C, illumination 120 μmol / m 2 ·s) and in a dark period (18:00-6:00, temperature 18°C) incubator. Thin out the seedlings to 1 plant after germination.

[0132] After the first true leaf unfolded, the following liquid (active oxygen reduction composition) was sprayed on the leaves of each plant in each treatment area (application of active oxygen reduction composition). 24 hours after the foliar spraying, a solution prepared by adding SILWET L-77 to the paraquat aqueous solution and diluting it 1000 times was sprayed on the leaves of each plant except the control area (T1) (application of paraquat).

[0133] ETR was measured by PAM fluorescence method 24 hours after the application of paraquat. In addition, SPAD value was measured using a chlorophyll meter 24 hours after the application of paraquat.

[0134] The active oxygen reduction composition and operation outlines of each treatment zone in Experimental Example 4 are shown in Table 4 below.

[0135]

[0136] Figure 7 The ETR when PAR is set to 1496 is shown in FIG. Figure 8 The SPAD values ​​are shown in Figure 7It is clear that in T2, ETR was significantly lower than in T1. In T3, where the composition for reducing active oxygen species containing SL was applied, ETR was slightly higher than in T2, and in T5, where the composition for reducing active oxygen species containing SL and lecithin was applied, ETR was significantly higher than in T2, indicating that ROS was sufficiently reduced compared to T2. Figure 8 The SPAD values ​​also show the same result.

[0137] [Experimental Example 5]

[0138] A 9 cm plastic pot was filled with soil, and three soybeans (Fukuyutaka) were sown.

[0139] After sowing, the seeds were grown in a light period (6:00-18:00, temperature 26°C, illumination 120 μmol / m 2 ·s) and in a dark period (18:00-6:00, temperature 18°C) incubator. Thin out the seedlings to 1 plant after germination.

[0140] After the first true leaf unfolded, the following liquid (active oxygen reduction composition) was sprayed on the leaves of each plant in each treatment area (application of active oxygen reduction composition). 24 hours after the foliar spraying, a solution prepared by adding SILWET L-77 to the paraquat aqueous solution and diluting it 1000 times was sprayed on the leaves of each plant except the control area (T1) (application of paraquat).

[0141] ETR was measured by PAM fluorescence method 24 hours after the application of paraquat. In addition, SPAD value was measured using a chlorophyll meter 24 hours after the application of paraquat.

[0142] The active oxygen reduction composition and operation outlines of each treatment zone in Experimental Example 5 are shown in Table 5 below.

[0143]

[0144] Fig. 9 The ETR when PAR is set to 1493 is shown in FIG. Fig.10 The SPAD values ​​are shown in Fig. 9 It is clear that in T2, ETR was significantly lower than in T1. In T3 to T5, where the composition not belonging to the present invention was sprayed, no increase in ETR was observed compared to T2, indicating that ROS was not reduced compared to T2. Fig.10 The SPAD values ​​also show the same result.

[0145] [Experimental Example 6]

[0146] A 9 cm plastic pot was filled with soil, and three soybeans (Fukuyutaka) were sown.

[0147] After sowing, the seeds were grown in a light period (6:00-18:00, temperature 26°C, illumination 120 μmol / m 2 ·s) and in a dark period (18:00-6:00, temperature 18°C) incubator. Thin out the seedlings to 1 plant after germination.

[0148] After the first true leaf unfolded, the following liquid (active oxygen reduction composition) was sprayed on the leaves of each plant in each treatment area (application of active oxygen reduction composition). 24 hours after the foliar spraying, a solution prepared by adding SILWET L-77 to the paraquat aqueous solution and diluting it 1000 times was sprayed on the leaves of each plant except the control area (T1) (application of paraquat).

[0149] ETR was measured by PAM fluorescence method 24 hours after the application of paraquat. In addition, SPAD value was measured using a chlorophyll meter 24 hours after the application of paraquat.

[0150] The active oxygen reduction composition and operation outlines of each treatment zone in Experimental Example 6 are shown in Table 6 below.

[0151]

[0152] Fig.11 The ETR when PAR is set to 1496 is shown in FIG. Fig.12 The SPAD values ​​are shown in Fig.11 It is clear that in T2, ETR was significantly lower than in T1. In T3 with low SF concentration and T4 with low KVP concentration, ETR was slightly higher than in T2, and in T5 where the active oxygen reduction composition containing diluted SF and KVP was sprayed, ETR was significantly higher than in T2, indicating that ROS was sufficiently reduced compared to T2.

[0153] [Experimental Example 7]

[0154] The 9 cm plastic pot was filled with soil and 3 soybeans (Fukuyutaka) were sown.

[0155] After sowing, the seeds were grown in a light period (6:00-18:00, temperature 26°C, illumination 120 μmol / m 2 ·s) and in a dark period (18:00-6:00, temperature 18°C) incubator. Thin out the seedlings to 1 plant after germination.

[0156] After the first true leaf unfolded, the following liquid (active oxygen reduction composition) was sprayed on the leaves of each plant in each treatment area (application of active oxygen reduction composition). 24 hours after the foliar spraying, a solution prepared by adding SILWET L-77 to the paraquat aqueous solution and diluting it 1000 times was sprayed on the leaves of each plant except the control area (T1) (application of paraquat).

[0157] ETR was measured by PAM fluorescence method 24 hours after the application of paraquat. In addition, SPAD value was measured using a chlorophyll meter 24 hours after the application of paraquat.

[0158] The active oxygen reduction composition and operation outlines of each treatment zone in Experimental Example 7 are shown in Table 7 below.

[0159]

[0160] Fig.13 The ETR when PAR is set to 1496 is shown in FIG. Fig.14 The SPAD values ​​are shown in Fig.13 It is clear that in T2, ETR was significantly lower than in T1. In T2 and T3 to 6 where the composition for reducing active oxygen species containing SF and lecithin was applied, ETR was significantly higher than in T2, indicating that ROS was sufficiently reduced compared to T2. Fig.14 The SPAD values ​​also show the same result.

[0161] [Experimental Example 8]

[0162] A 9 cm plastic pot was filled with soil, and three soybeans (Fukuyutaka) were sown.

[0163] After sowing, the seeds were grown in a light period (6:00-18:00, temperature 26°C, illumination 120 μmol / m 2 ·s) and in a dark period (18:00-6:00, temperature 18°C) incubator. Thin out the seedlings to 1 plant after germination.

[0164] After the first true leaf unfolded, the following liquid (active oxygen reduction composition) was sprayed on the leaves of each plant in each treatment area (application of active oxygen reduction composition). 24 hours after the foliar spraying, a solution prepared by adding SILWET L-77 to the paraquat aqueous solution and diluting it 1000 times was sprayed on the leaves of each plant except the control area (T1) (application of paraquat).

[0165] ETR was measured by PAM fluorescence method 24 hours after the application of paraquat. In addition, SPAD value was measured using a chlorophyll meter 24 hours after the application of paraquat.

[0166] The active oxygen reduction composition and operation outlines of each treatment zone in Experimental Example 8 are shown in Table 8 below.

[0167]

[0168] Fig.15 The ETR when PAR is set to 1496 is shown in FIG. Fig.16 The SPAD values ​​are shown in Fig.15 It is clear that in T2, ETR was significantly lower than in T1. In T2 and T3 to 6 where the active oxygen reduction composition containing SF and KVP was sprayed, ETR was significantly higher than in T2, indicating that ROS was sufficiently reduced compared to T2. Fig.16 The SPAD values ​​also show the same result.

[0169] [Experimental Example 9]

[0170] A 9 cm plastic pot was filled with soil, and three soybeans (Fukuyutaka) were sown.

[0171] After sowing, the seeds were grown in a light period (6:00-18:00, temperature 26°C, illumination 120 μmol / m 2 ·s) and in a dark period (18:00-6:00, temperature 18°C) incubator. Thin out the seedlings to 1 plant after germination.

[0172] After the first true leaf unfolded, the following liquid (active oxygen reduction composition) was sprayed on the leaves of each plant in each treatment area (application of active oxygen reduction composition). 24 hours after the foliar spraying, a solution prepared by adding SILWET L-77 to the paraquat aqueous solution and diluting it 1000 times was sprayed on the leaves of each plant except the control area (T1) (application of paraquat).

[0173] ETR was measured by PAM fluorescence method 24 hours after the application of paraquat. In addition, SPAD value was measured using a chlorophyll meter 24 hours after the application of paraquat.

[0174] The active oxygen reduction composition and operation outlines of each treatment zone in Experimental Example 9 are shown in Table 9 below.

[0175]

[0176] Fig.17 The ETR when PAR is set to 1455 is shown in FIG. Fig.18 The SPAD values ​​are shown in Fig.17It is clear that in T2, ETR was significantly lower than in T1. In T2 and T3 where the composition for reducing active oxygen species containing SF was spread, ETR was significantly higher than in T2, indicating that ROS was sufficiently reduced compared to T2. In T2 and T5 where the composition for reducing active oxygen species containing SF and KVP was spread, ETR was significantly higher than in T2, indicating that ROS was sufficiently reduced compared to T2. In addition, an increase in ETR was confirmed in T5 compared to T3 and T4. According to Fig.18 The SPAD values ​​also show the same result.

[0177] The upper limit and / or lower limit of the numerical range recorded in this specification can be arbitrarily combined to limit the preferred range. For example, the upper limit and lower limit of the numerical range can be arbitrarily combined to limit the preferred range, the upper limit of the numerical range can be arbitrarily combined with each other to limit the preferred range, and the lower limit of the numerical range can also be arbitrarily combined with each other to limit the preferred range. In addition, in this application, the numerical range represented by the symbol "~" includes the numerical values ​​recorded before and after the symbol "~" as the lower limit and upper limit, respectively.

[0178] Throughout this specification, unless otherwise specified, expressions in the singular should also be understood to include the concept of its plural form. Therefore, unless otherwise specified, articles in the singular (for example, "a", "an", "the", etc. in English) should also be understood to include the concept of its plural form.

[0179] Although the present embodiment has been described in detail above, the specific configuration is not limited to these embodiments, and design changes within the scope that does not depart from the gist of the present disclosure are also included in the present disclosure.

[0180] All publications, patents and patent applications cited in this specification are incorporated herein by direct reference.

Claims

1. A composition for reducing active oxygen, comprising: at least one biosurfactant selected from a peptide biosurfactant and a sugar biosurfactant, and an agriculturally acceptable carrier.

2. The composition for reducing active oxygen according to claim 1, wherein The biosurfactant is contained in an amount of 0.004 to 0.2% by mass based on 100% by mass of the active oxygen reducing composition. The composition for reducing active oxygen species according to claim 1 , comprising a phospholipid.

4. The composition for reducing active oxygen according to claim 3, wherein The above-mentioned active oxygen reduction composition contains 0.004 to 0.2 mass % of the above-mentioned biosurfactant in an amount of 100 mass %, The phospholipid is contained in an amount of 0.002 to 0.1% by mass in 100% by mass of the active oxygen reducing composition. The composition for reducing active oxygen according to claim 1, comprising a soybean meal decomposition product.

6. The composition for reducing active oxygen according to claim 5, wherein The above-mentioned active oxygen reduction composition contains 0.004 to 0.2 mass % of the above-mentioned biosurfactant in an amount of 100 mass %, The above-mentioned active oxygen reducing composition contains 0.004 to 1.0% by mass of the above-mentioned soybean meal decomposition product in 100% by mass.

7. The composition for reducing active oxygen according to claim 1, wherein The biosurfactant is at least one biosurfactant selected from the group consisting of surfactin, rhamnolipid, sophorolipid, and salts thereof. The composition for reducing active oxygen species according to claim 1, which is used for spreading on leaves of plants. 9 . A precursor of a composition for reducing active oxygen species, which becomes the composition for reducing active oxygen species according to claim 1 by dilution.

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