Breeding method for meat and egg dual-purpose type flower-neck green-shell Puan disc river black-bone chicken commercial line

Abstract

The invention discloses a meat and egg dual-purpose flower-neck and green-shell Puan pan River black-bone chicken commercial line breeding method, which comprises the following steps: introducing the blood relationship of a speckle-feather black-bone chicken and a bamboo-town chicken on the basis of the blood relationship of Puan pan River black-bone chicken, and adopting a matching combination ZX * [(MW * PA) * MW] to breed the meat and egg dual-purpose flower-neck and green-shell Puan pan River black-bone chicken; the obtained commercial generation of the commercial line of the flower-neck green-shell Pu'an disc river black-bone chicken has the appearance characteristics of flower necks, black feathers, black skins, black shins, black crowns and black meat, is ideal in appearance, wide and large in body form, high in growth speed, early in marketing day age, high in meat yield, capable of producing emerald green-shell eggs and suitable for both meat and eggs, and is superior to pure lines of Pu'an disc river black-bone chickens in weight, growth speed, slaughter performance, reproductive performance and marketing day age; meanwhile, an individual selection and family selection method is adopted, and the meat and egg dual-purpose flower-neck green-shell Puanpan river black-bone chicken commercial line which is ideal in appearance, wide and large in body type, high in growth speed, early in marketing day age, high in meat yield, capable of producing emerald-green-shell eggs and stable in performance can be accurately selected. The method is suitable for popularization and application in the technical field of poultry breeding.

Description

Technical Field

[0001] The invention relates to the technical field of poultry breeding, and in particular to a method for producing a breeding stock of a meat-and-egg dual-purpose flower-necked green-shell Pu'an Panjiang black-bone chicken matching system. Background Art

[0002] In recent years, the policy system for seed industry revitalization has been continuously improved. As a strategic fulcrum for seed industry revitalization, livestock and poultry germplasm resource innovation has become the core driving force for ensuring national food security and driving agricultural transformation and upgrading.

[0003] Local chickens have the characteristics of excellent meat and egg quality, but the breeding cycle is long and the production performance is low. Improving the growth rate, shortening the age of listing, and increasing egg production can effectively reduce the comprehensive breeding cost of local chickens, which is one of the cost-cutting and efficiency-increasing measures to cope with the high cost of feed raw materials. At the same time, the unique eggshell color (such as the natural green color of green-shelled eggs) not only meets consumers' aesthetic needs for product differentiation, but also conveys the quality association of "high nutrition" and "ecological breeding" through visual cognition, making the price of green-shelled eggs 2-4 times that of ordinary eggs. Therefore, improving the eggshell color has become a new way to improve the economic benefits of a limited number of egg products.

[0004] Pu'an Panjiang Black Chicken is named after the Beipanjiang River Basin in Pu'an County, Qianxinan Prefecture, Guizhou Province, where it is mainly produced. It is also known as Pu'an Niujiaoshan Black Bone Chicken and Pu'an Black Golden Chicken. The ancient Miao people used it for sacrifices and "cockfighting" on important festivals. After a long period of natural and artificial selection, it has gradually formed a special black chicken variety that can be used as both meat and medicine and can withstand rough feeding. It has the characteristics of black feathers, black shanks, black skin, black meat, black bones, strong resistance to adversity, and delicious meat and eggs. It has become the main variety promoted by the local ecological poultry industry. However, the current development and utilization of Pu'an Panjiang Black Chicken is still very low, and the loss of germplasm resources and the lack of systematic protection mechanisms are becoming increasingly serious. There are problems such as slow growth, small body weight in the early stage, long feeding cycle, low egg production, and small egg weight, which seriously restrict the high-quality development of the industry. Therefore, it is very necessary to carry out germplasm innovation for the local resource Pu'an Panjiang Black Chicken.

[0005] Zhuxiang Chicken is named after the city of Chishui in Zunyi, Guizhou Province, which is the "hometown of bamboo". It is a local breed that can be used for both meat and eggs. Because the locals prefer black-bone individuals, they are intentionally selected and retained, resulting in the appearance characteristics of black hair, skin, meat, bones, beaks, crowns, and toes. It has high egg-laying performance and some groups can produce green-shelled eggs. It is an extremely precious and highly plastic breeding material, but its current development and utilization level is still low. The hemp-feathered black chicken has excellent meat and egg-laying performance, grows fast and weighs heavily. It has the appearance characteristics of red hemp feathers, black bones, black skin, and black legs, and can be used to improve the shortcomings of local breeds such as slow growth and low weight.

[0006] Therefore, by utilizing the advantages of the above materials, further hybridization and improvement of the Pu'an Panjiang black chicken can be carried out to shorten the age of market, improve egg-laying performance, and cultivate the Pu'an Panjiang black chicken with a flower neck and green shell that can be used for both meat and eggs to meet the needs of the southwest and south China regions. Summary of the invention

[0007] The technical problem to be solved by the present invention is to provide a method for producing a matching breed of Pu'an Panjiang black-bone chickens with flower-necked green shells, which are both meat- and egg-purpose and have ideal appearance, large body shape, fast growth rate, early market age, high meat production, emerald green shell eggs, and stable performance.

[0008] The technical solution adopted by the present invention to solve the technical problem is: the method for producing seeds of the matching system of the meat and egg dual-purpose flower-neck green-shell Pu'an Panjiang black-bone chicken comprises the following steps:

[0009] 1) Paternal breeding:

[0010] 101) Select the existing Zhuxiang Chicken ZX line, which meets the weight and appearance standards of 8 and 16 weeks of age, and the hens that meet the weight and appearance standards of 8 and 16 weeks of age, the egg production standard of 43 weeks of age, and the appearance standards, and breed them according to the weight and appearance standards of 8 and 16 weeks of age for roosters, and the weight and appearance standards of 8 and 16 weeks of age for hens, and the egg production standard of 43 weeks of age, and the appearance standards, to obtain the great-grandfather generation ZX paternal line male parent and the great-grandfather generation ZX paternal line female parent;

[0011] 102) Breeding the great-grandfather ZX paternal line father and the great-grandfather ZX paternal line mother, and selecting and breeding according to the weight standard and appearance standard of roosters at 8 and 16 weeks of age and the weight standard and egg production standard and appearance standard of hens at 8 and 16 weeks of age and 43 weeks of age to obtain the grandparent ZX paternal line father and the grandparent ZX paternal line mother;

[0012] 103) The grandparent ZX paternal line male parent and the grandparent ZX paternal line female parent are mated and selected according to the weight standard and appearance standard of roosters at 8 and 16 weeks of age, the semen quality standard of roosters at 30 weeks of age, and the positive homozygous genotype of green shell eggs to obtain the parent ZX paternal line male parent;

[0013] 2) Maternal breeding:

[0014] 201) Select existing purebred black-bone chicken MW line that meets the weight standard and appearance standard at 8 and 16 weeks of age and the hens that meet the weight standard at 8 and 16 weeks of age and the egg production standard at 43 weeks of age and the appearance standard for breeding, and carry out successive breeding according to the weight standard and appearance standard of the rooster at 8 and 16 weeks of age to obtain the great-grandparent MW maternal father and the grandparent MW maternal mother;

[0015] 202) Breed the existing purebred Pu'an Panjiang black-bone chicken PA series roosters that meet the weight and appearance standards at 8 and 16 weeks of age and hens that meet the weight standards at 8 and 16 weeks of age and the egg production and appearance standards at 43 weeks of age, and select and breed the great-grandparent PA maternal line females according to the egg production and appearance standards of the hens at 43 weeks of age;

[0016] 203) The great-grandfather MW maternal sire and the great-grandfather PA maternal sire are mated and selected according to the weight standard and appearance standard of roosters at 8 and 16 weeks of age to obtain the grandparent MP maternal sire;

[0017] 204) The grandparent MP maternal line male parent and the grandparent MW maternal line female parent were backcrossed and bred according to the weight standards of hens at 8 and 16 weeks of age and the egg production and appearance standards at 43 weeks of age to obtain the parental MM maternal line female parent;

[0018] 3) The ZX paternal parent obtained by paternal selection and the MM maternal parent obtained by maternal selection can be crossed to obtain the flower-necked green-shell Pu'an Panjiang black-bone chicken.

[0019] Furthermore, in steps 101) to 103), the selection and breeding of the great-grandparent ZX paternal father, the great-grandparent ZX paternal mother, the grandparent ZX paternal father, the grandparent ZX paternal mother, and the parent ZX paternal father are all carried out by single cage rearing and artificial insemination for pure breeding.

[0020] Furthermore, in steps 201) to 204), the breeding of the great-grandparent MW maternal sire, the great-grandparent PA maternal sire, the grandparent MP maternal sire, the grandparent MW maternal sire, and the parent MM maternal sire are all carried out by hybridization using single cage breeding and artificial insemination.

[0021] Furthermore, in steps 101) to 103), the weight standard of the Zhuxiang chicken rooster at 8 weeks of age is 0.7 kg, the weight standard at 16 weeks of age is 1.75 kg, the egg production standard at 43 weeks of age is that the same family hen lays more than 80 eggs at 43 weeks of age, the appearance characteristics of the Zhuxiang chicken rooster are strong and solid, feathers with dark green metallic luster, well-developed sickle feathers, black feathers, black skin, black shanks, black crown, and black meat, and the semen quality requirement of the Zhuxiang chicken rooster is a single semen collection volume of more than 0.5 mL , milky white, thick, pH of about 7 to 8, density of 3 to 5 billion / mL, vitality of more than 0.7, fertilization rate of more than 90%; the egg production standard of the Zhuxiang chicken hen at 43 weeks of age is an average egg production of more than 80 eggs, the eggshell color is green, and the average egg weight is 50g. The weight standard of the Zhuxiang chicken hen at 8 weeks of age is 0.65kg, and the weight standard at 16 weeks of age is 1.3kg. The appearance characteristics of the Zhuxiang chicken hen are delicate body shape, symmetrical structure, black feathers, black skin, black legs, black crown, and black meat.

[0022] Furthermore, in step 201), the weight standard of the black-bone chicken rooster at 8 weeks of age is 1.5 kg, the weight standard at 16 weeks of age is 2.4 kg, the egg production standard at 43 weeks of age is an average egg production of hens of the same family at 43 weeks of age of more than 85 eggs, and the appearance standard of the black-bone chicken rooster is as follows: a broad and strong physique, a large upright crimson single comb, well-developed sickle feathers, red hemp feathers all over the body, black wing feathers and sickle feathers, black skin, shanks, and meat, and the semen quality requirement of the black-bone chicken rooster is a single The semen collected is more than 0.7 mL, the semen is milky white and thick, the pH is about 7 to 8, the density is 3 to 5 billion / mL, the vitality is more than 0.7, and the fertilization rate is more than 90%; the weight standard of the black-feathered chicken hen at 8 weeks of age is 1 kg, the weight standard at 16 weeks of age is 1.6 kg, the egg production standard at 43 weeks of age is more than 85 eggs, and the eggshell color is pink, with an average egg weight of 54 g; the appearance standard of the black-feathered chicken hen is a wide and round body, a dark red upright single comb, jute feathers all over the body, and black skin, tibia, and meat.

[0023] Furthermore, in step 202), the weight standard of the Pu'an Panjiang black-bone chicken rooster at 8 weeks of age is 0.5 kg, the weight standard at 16 weeks of age is 1.4 kg, the egg production standard at 43 weeks of age is an average egg production of hens of the same family at 43 weeks of age of more than 65 eggs, the appearance standard of the Pu'an Panjiang black-bone chicken rooster is a strong physique, black feathers, black skin, black shanks, black crown, and black meat, and the semen quality requirement of the Pu'an Panjiang black-bone chicken rooster is a single semen collection volume of more than 0.5 mL, Milky white, thick, pH of about 7 to 8, density of 3 to 5 billion / mL, vitality above 0.7, fertilization rate above 90%; the weight standard of the Pu'an Panjiang black-bone chicken hen at 8 weeks of age is 0.45kg, the weight standard at 16 weeks of age is 1.2kg, the egg production standard at 43 weeks of age is more than 65 eggs and the eggshell color is pink, the average egg weight is 48g, and the appearance standard of the Pu'an Panjiang black-bone chicken hen is medium size, black feathers, black skin, black legs, black crown, and black meat.

[0024] Furthermore, in step 203), the weight standard of the MP rooster at 8 weeks of age is 1.2 kg, and the weight standard of the MP rooster at 16 weeks of age is 1.9 kg. The appearance standard of the MP rooster is a broad and strong physique, a large upright single purple-black comb, red and yellow comb feathers and a small part of the back feathers, black feathers on the wings, abdomen, and tail, black skin, black shanks, and black meat.

[0025] Furthermore, in step 204), the weight standard of the MM hen at 8 weeks of age is 0.8 kg, the weight standard at 16 weeks of age is 1.5 kg, the egg production standard at 43 weeks of age is more than 75 eggs and the eggshell color is pink, and the appearance standard of the MM hen is medium size, purple-black upright single comb, jute neck feathers, chest and part of the back feathers are jute color, wings, abdomen and tail feathers are all black, black skin, black shanks and black meat.

[0026] Furthermore, in step 3), the obtained Pu'an Panjiang black-bone chicken with a flowery neck and green shell has the appearance characteristics of "flowery neck, black feathers, black skin, black shanks, black crown, and black meat": large body, plump body, wide and deep chest, full abdomen, black upright single crown, overall feathers are black with dark green metallic luster, the feathers on the neck are reddish yellow, and the shanks, skin, and meat are all black; the obtained Pu'an Panjiang black-bone chicken with a flowery neck and green shell male and female are marketed as high-quality broilers at the age of 16 weeks; the hens are sold as local high-quality old hens after the egg production decreases at the age of 43 weeks.

[0027] Furthermore, before mating, the breeder chickens in the matching system are purified from salmonella and chicken leukemia.

[0028] The invention has the following beneficial effects: the method for producing a matching system of Pu'an Panjiang black-bone chicken with flower neck and green shell for both meat and eggs introduces the bloodline of Mayu black-bone chicken and Zhuxiang chicken on the basis of the bloodline of Pu'an Panjiang black-bone chicken, and adopts the matching combination ZX×[(MW×PA)×MW], that is, the Mayu black-bone chicken rooster is hybridized with the Pu'an Panjiang black-bone chicken hen, so as to improve the meat performance and egg-laying performance of the offspring under good hybrid vigor; and then the Mayu black-bone chicken hen is used for a backcrossing to further improve the meat performance and egg-laying performance of the hybrid offspring; and the Zhuxiang chicken rooster is further used as the terminal paternal parent, and the obtained commercial generation inherits the green-shell egg gene of the terminal paternal parent, and the hens have excellent egg-laying performance and produce emerald green eggs with higher economic benefits, and can be sold as high-quality old hens after the egg-laying is completed; the meat-and-egg matching type Pu'an Panjiang black-bone chicken obtained by the invention contains Pu'an Panjiang The bloodline of black chicken is 12.5%, the bloodline of Zhuxiang chicken is 50%, and the bloodline of Mawu chicken is 37.5%. The commercial generation has the ideal and unique appearance characteristics of "flower neck, black feathers, black skin, black legs, black crown, and black meat". The adult chickens have ideal body shape and appearance, complete disease elimination, and excellent production performance. The present invention gives full play to the high-yield performance of the great-grandparent maternal father and the grandparent maternal mother Mayu black chicken MW series, which are heavy, early on the market age, excellent meat performance, high egg production, and large egg weight, so that the growth rate, market weight, egg production at 43 weeks of age, and egg weight of the flower-necked green-shell Pu'an Panjiang black chicken for both meat and eggs are significantly improved, and the market age is effectively shortened; the green shell egg gene of the terminal father Zhuxiang chicken ZX series and the production performance with higher egg-laying performance are inherited, so that the egg-laying performance of the commercial generation hens is excellent and the production of emerald green shell eggs with higher economic value is achieved. The meat-and-egg dual-purpose Pu'an Panjiang black-bone chicken of the present invention has a large body, a plump body, a broad and deep chest, a full abdomen, a black upright single comb, black feathers with a dark green metallic luster, reddish-yellow feathers on the neck, black shanks, skin and meat, and has the unique appearance characteristics of "flower neck, black feathers, black skin, black shanks, black combs and black meat". Both male and female chickens have excellent meat performance and can be marketed as high-quality broilers at the age of 16 weeks. The weight of 16-week-old roosters is more than 2 kg, and the weight of hens is more than 1 kg. The chicken weighs more than 1.4kg, 38 days earlier than the 150-day-old purebred Pu'an Panjiang black-bone chicken, and the growth rate is significantly improved; the hens produce emerald green shell eggs with higher economic benefits than pink shell eggs before 43 weeks of age, which can effectively alleviate the imbalance between supply and demand in the green shell egg market and meet consumers' preference for eggs of different shell colors; the average egg weight is 52g, which is significantly higher than the purebred Pu'an Panjiang black-bone chicken, and has a significant improvement effect on egg quality performance such as egg weight and eggshell color. The egg production at 43 weeks of age is 78 eggs, 13 more than the purebred Pu'an Panjiang black-bone chicken, which significantly improves the egg-laying performance. Furthermore, after the egg production of hens decreases at 43 weeks of age, they can be sold as high-quality old hens with local characteristics, achieving both meat and egg production.Therefore, the meat-and-egg dual-purpose Pu'an Panjiang black-bone chicken obtained by the present invention has an ideal body shape and appearance, is completely free of diseases, and has excellent production performance; the meat-and-egg dual-purpose Pu'an Panjiang black-bone chicken obtained by the present invention has the appearance characteristics of a flower neck, black feathers, black skin, black shanks, black crown, and black meat, has an ideal appearance, a large body, a fast growth rate, an early age to market, a high meat production, produces emerald green shell eggs, and can be used for both meat and eggs, and its weight, growth rate, slaughter performance, reproductive performance, and age to market are all superior to those of the pure strain of Pu'an Panjiang black-bone chicken. At the same time, the present invention adopts individual selection and family selection methods, and can accurately select the meat-and-egg dual-purpose Pu'an Panjiang black-bone chicken matching system with an ideal appearance, a large body, a fast growth rate, an early age to market, a high meat production, produces emerald green shell eggs, and has stable performance. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It is a schematic diagram of the method for producing seeds of the meat-egg dual-purpose flower-neck green-shell Pu'an Panjiang black-bone chicken matching system of the present invention;

[0030] Figure 2 This is a characteristic diagram of the appearance of the Pu'an Panjiang black-bone chicken with a flower neck and green shell for both meat and eggs according to the present invention. DETAILED DESCRIPTION

[0031] like Figure 1-2 As shown, the method for producing seeds of the meat-egg dual-purpose flower-neck green-shell Pu'an Panjiang black-bone chicken matching system comprises the following steps:

[0032] 1) Paternal breeding:

[0033] 101) Select the existing Zhuxiang Chicken ZX line, which meets the weight and appearance standards of 8 and 16 weeks of age, and the hens that meet the weight and appearance standards of 8 and 16 weeks of age, the egg production standard of 43 weeks of age, and the appearance standards, and breed them according to the weight and appearance standards of 8 and 16 weeks of age for roosters, and the weight and appearance standards of 8 and 16 weeks of age for hens, and the egg production standard of 43 weeks of age, and the appearance standards, to obtain the great-grandfather generation ZX paternal line male parent and the great-grandfather generation ZX paternal line female parent;

[0034] 102) Breeding the great-grandfather ZX paternal line father and the great-grandfather ZX paternal line mother, and selecting and breeding according to the weight standard and appearance standard of roosters at 8 and 16 weeks of age and the weight standard and egg production standard and appearance standard of hens at 8 and 16 weeks of age and 43 weeks of age to obtain the grandparent ZX paternal line father and the grandparent ZX paternal line mother;

[0035] 103) The grandparent ZX paternal line male parent and the grandparent ZX paternal line female parent are mated and selected according to the weight standard and appearance standard of roosters at 8 and 16 weeks of age, the semen quality standard of roosters at 30 weeks of age, and the positive homozygous genotype of green shell eggs to obtain the parent ZX paternal line male parent;

[0036] In steps 101) to 103), the weight standard of the Zhuxiang chicken rooster at 8 weeks of age is 0.7 kg, the weight standard at 16 weeks of age is 1.75 kg, the egg production standard at 43 weeks of age is that the same family hen lays more than 80 eggs at 43 weeks of age, the appearance characteristics of the Zhuxiang chicken rooster are strong and solid, the feathers have a dark green metallic luster, the sickle feathers are well developed, the feathers, the skin, the legs, the crown and the meat are black, and the semen quality requirements of the Zhuxiang chicken rooster are that the single semen collection volume is more than 0.5 mL, and the milky white The egg production standard of the Zhuxiang chicken hen at 43 weeks of age is 80 eggs or more, the eggshell color is green, and the average egg weight is 50g. The weight standard of the Zhuxiang chicken hen at 8 weeks of age is 0.65kg, and the weight standard at 16 weeks of age is 1.3kg. The appearance characteristics of the Zhuxiang chicken hen are delicate body shape, symmetrical structure, black feathers, black skin, black legs, black crown, and black meat. The green-shell egg positive homozygous genotype rooster is obtained in the following manner: according to the characteristic that the green-shell egg trait is autosomal dominant inheritance, a group detection of Zhuxiang chickens is carried out by molecular marker technology, and the green-shell egg positive homozygous genotype is selected to obtain a ZX line with a green-shell egg rate of 100%; wherein the molecular marker technology is implemented by a patented technology with application number 201510194180.6, application date 2015-04-22, and patent name "Primer composition for identifying the genotype of green-shell laying hens and its application";

[0037] 2) Maternal breeding:

[0038] 201), select existing purebred Ma Yu black chicken MW lineage roosters that meet the 8- and 16-week-old weight and appearance standards and hens that meet the 8- and 16-week-old weight standards and the 43-week-old egg production standards and appearance standards for breeding, and carry out successive breeding according to the 8- and 16-week-old weight and appearance standards for the roosters, to obtain the great-grandparent MW maternal father and the grandparent MW maternal mother; the 8-week-old weight standard of the Ma Yu black chicken rooster is 1.5 kg, the 16-week-old weight standard is 2.4 kg, the 43-week-old egg production standard is the average egg production of hens of the same family at 43 weeks of age is more than 85 eggs, and the appearance standards of the Ma Yu black chicken rooster are as follows: a broad and strong physique, a dark red upright large Single comb, well-developed sickle feathers, whole body feathers are red jute color, wing feathers and sickle feathers are black, skin, shanks and meat are black. The semen quality requirements of the black-feathered chicken rooster are a single semen collection volume of more than 0.7mL, milky white, thick, pH of about 7-8, density of 3-5 billion / mL, vitality of more than 0.7, and fertilization rate of more than 90%; the weight standard of the black-feathered chicken hen at 8 weeks of age is 1kg, the weight standard at 16 weeks of age is 1.6kg, the egg production standard at 43 weeks of age is more than 85 eggs and the eggshell color is pink, the average egg weight is 54g, and the appearance standard of the black-feathered chicken hen is a wide and round body, a dark red upright single comb, jute feathers all over the body, and black skin, shanks and meat.

[0039] 202) The existing purebred Pu'an Panjiang black-bone chicken PA series roosters that meet the weight standards and appearance standards at 8 and 16 weeks of age and hens that meet the weight standards at 8 and 16 weeks of age and the egg production standards and appearance standards at 43 weeks of age are bred, and the great-grandparent PA maternal line female parent is selected according to the egg production standards and appearance standards for the hens at 43 weeks of age; the weight standard for the Pu'an Panjiang black-bone chicken roosters at 8 weeks of age is 0.5 kg, the weight standard at 16 weeks of age is 1.4 kg, the egg production standard at 43 weeks of age is an average egg production of more than 65 eggs for hens of the same family at 43 weeks of age, and the appearance standard for the Pu'an Panjiang black-bone chicken roosters is a healthy physique. Strong, black feathers, black skin, black shanks, black crown, black meat. The semen quality requirements of the Pu'an Panjiang black-bone chicken rooster are a single semen collection volume of more than 0.5mL, milky white, thick, pH of about 7-8, density of 3-5 billion / mL, vitality of more than 0.7, and fertilization rate of more than 90%; the weight standard of the Pu'an Panjiang black-bone chicken hen at 8 weeks of age is 0.45kg, the weight standard at 16 weeks of age is 1.2kg, the egg production standard at 43 weeks of age is more than 65 eggs, and the eggshell color is pink, with an average egg weight of 48g. The appearance standard of the Pu'an Panjiang black-bone chicken hen is a medium size, black feathers, black skin, black shanks, black crown, and black meat.

[0040] 203) The great-grandparent MW maternal sire and the great-grandparent PA maternal sire were mated and selected according to the weight standards and appearance standards of roosters at 8 and 16 weeks of age to obtain the grandparent MP maternal sire; the weight standard of the MP rooster at 8 weeks of age is 1.2 kg, and the weight standard at 16 weeks of age is 1.9 kg. The appearance standard of the MP rooster is a broad and strong physique, a large upright purple-black single comb, the comb feathers and a small part of the back feathers are red and yellow, the wings, abdomen, and tail feathers are all black, and the skin, legs, and meat are black.

[0041] 204) The grandparent MP maternal sire and the grandparent MW maternal dam are backcrossed and bred according to the weight standards of hens at 8 and 16 weeks of age, the egg production standard at 43 weeks of age, and the appearance standards to obtain the parental MM maternal sire; the weight standard of the MM hen at 8 weeks of age is 0.8 kg, the weight standard at 16 weeks of age is 1.5 kg, the egg production standard at 43 weeks of age is more than 75 eggs and the eggshell color is pink; the appearance standard of the MM hen is a medium size, a purple-black upright single comb, jute neck feathers, chest and part of the back feathers are jute color, the wings, abdomen and tail feathers are all black, and the skin, legs and meat are black.

[0042] 3) The parental generation ZX paternal line sire obtained by paternal selection and the parental generation MM maternal line maternal line obtained by maternal selection can be crossed to obtain the flower-necked green-shell Pu'an Panjiang black-bone chicken. The obtained flower-necked green-shell Pu'an Panjiang black-bone chicken has the appearance characteristics of "flower-necked, black feathers, black skin, black shanks, black crown, and black meat": large body, plump body, wide and deep chest, full abdomen, black upright single crown, overall feathers are black with dark green metallic luster, neck feathers are red and yellow, shanks, skin, and meat are all black; the obtained flower-necked green-shell Pu'an Panjiang black-bone chicken male and female chickens are listed as high-quality broilers at the age of 16 weeks; the weight of roosters at the age of 16 weeks reaches more than 2kg, and the weight of hens reaches more than 1.4kg; hens produce emerald green shell eggs before 43 weeks of age, with an average egg weight of 52g, and an egg production of 78 eggs at 43 weeks of age. After the egg production decreases at 43 weeks of age, they are sold as high-quality old hens with local characteristics.

[0043] In the above implementation, in order to ensure the systematic breeding of the dual-purpose meat and egg type flower neck green shell Pu'an Panjiang black chicken, in steps 101) to 103), the selection and breeding of the great-grandparent generation ZX paternal father, the great-grandparent generation ZX paternal mother, the grandparent generation ZX paternal father, the grandparent generation ZX paternal mother, and the parent generation ZX paternal father are all carried out by single cage breeding and artificial insemination. Further, in steps 201) to 204), the selection and breeding of the great-grandparent generation MW maternal father, the great-grandparent generation PA maternal mother, the grandparent generation MP maternal father, the grandparent generation MW maternal mother, and the parent generation MM maternal mother are all carried out by single cage breeding and artificial insemination.

[0044] Furthermore, in order to ensure the healthy production of the meat-and-egg dual-purpose green-shell Pu'an Panjiang black-bone chicken, the breeding chickens in the matching system are purified from Salmonella and chicken leukemia before breeding.

[0045] The present invention statistically analyzes the overall appearance characteristics, growth performance, egg-laying performance and slaughter performance of the obtained meat-egg dual-purpose flower-necked green-shell Pu'an Panjiang black-bone chicken. Its appearance characteristics are shown in Table 1, its growth performance is shown in Table 2, its egg-laying performance is shown in Table 3, and its slaughter performance is shown in Table 4:

[0046] Table 1 Appearance characteristics

[0047]

[0048] Table 2 Growth performance

[0049] Age / W Rooster weight / g Hen / g 0 36.82±2.45 35.41±2.16 2 186.64±12.97 166.08±8.94 4 383.73±20.43 330.65±14.87 6 586.34±29.42 495.31±18.53 8 1010.85±50.84 875.45±43.79 10 1400.03±65.71 1028.09±52.44 12 1771.46±73.60 1164.67±64.04 14 1947.88±79.19 1350.11±74.32 16 2088.13±89.82 1474.92±79.08

[0050] Table 3 Egg laying performance

[0051] index Numeric Age at first calving / d 147.56±10.78 Weight at birth / g 1835.42±61.85 Egg weight at start / g 42.01±2.83 Egg weight at 43 weeks old / g 52.16±3.79 Egg production at 43 weeks 78.22±12.94 43 weeks old body weight / g 2194.35±104.47 Eggshell color Emerald Green

[0052] Table 4 Slaughter performance

[0053] index 16 weeks old rooster 16 week old hens Weight before slaughter / g 2075.59±67.54 1498.21±50.02 Slaughter rate / % 89.06±0.87 90.13±0.66 Half-eviscerated rate 81.87±1.05 81.14±0.92 Full evisceration rate 76.22±1.57 70.73±1.39 Chest muscle rate / % 15.25±0.72 17.94±1.01 Leg muscle rate / % 26.26±0.83 21.74±0.96 Abdominal fat rate / % 0.68±0.32 3.76±1.21

[0054] As can be seen from Tables 1 to 4, the commercial generation of Pu'an Panjiang black-bone chickens with flower necks and green shells for both meat and eggs obtained by the present invention are large in size, plump in body, with broad and deep chest, plump abdomen, black upright single comb, black feathers with dark green metallic luster, reddish yellow feathers on the neck, black shanks, skin and meat, and have the unique appearance characteristics of "flower neck, black feathers, black skin, black shanks, black combs and black meat". Both male and female chickens have excellent meat performance and can be put on the market as high-quality broilers at the age of 16 weeks. The weight of roosters at the age of 16 weeks can reach The weight of the hen is more than 2kg, and the weight of the hen is more than 1.4kg, which is 38 days earlier than the 150-day-old purebred Pu'an Panjiang black-bone chicken, and the growth rate is significantly improved; the hen produces emerald green shell eggs with higher economic benefits than pink shell eggs before 43 weeks of age, which can effectively alleviate the imbalance between supply and demand in the green shell egg market and meet consumers' preference for eggs of different shell colors; the average egg weight is 52g, which is significantly higher than that of purebred Pu'an Panjiang black-bone chickens, and has a significant improvement effect on egg quality performance such as egg weight and eggshell color. The egg production at 43 weeks of age is 78 eggs, which is 13 more than that of purebred Pu'an Panjiang black-bone chickens, significantly improving the egg-laying performance. Furthermore, after the egg production of hens decreases at 43 weeks of age, they can be sold as high-quality old hens with local characteristics, achieving both meat and egg use. Therefore, the meat and egg dual-purpose flower neck green shell Pu'an Panjiang black-bone chicken obtained by the present invention has an ideal body shape and appearance, complete disease purification, and excellent production performance; the meat and egg dual-purpose flower neck green shell Pu'an Panjiang black-bone chicken matching system commercial generation has the appearance characteristics of flower neck, black feathers, black skin, black legs, black crown, and black meat, and has an ideal appearance, a large body, a fast growth rate, an early market age, a high meat production, and produces emerald green shell eggs, and is used for both meat and eggs. The weight, growth rate, slaughter performance, reproductive performance, and market age are all better than those of the pure line of Pu'an Panjiang black-bone chicken. Therefore, the present invention can provide better reference information for the breeding of the meat and egg dual-purpose flower neck green shell Pu'an Panjiang black-bone chicken matching system.

[0055] The above description shows and describes several preferred embodiments of the invention, but as mentioned above, it should be understood that the invention is not limited to the form disclosed herein, and should not be regarded as excluding other embodiments, but can be used in various other combinations, modifications and environments, and can be modified within the scope of the invention concept described herein through the above teachings or the technology or knowledge of the relevant field. Changes and modifications made by those skilled in the art should be within the scope of protection of the claims attached to the invention without departing from the spirit and scope of the invention.

Claims

1. A method for producing a matching line of Pu'an Panjiang black-bone chickens with flower neck and green shell for both meat and eggs, comprising the following steps: 1) Paternal breeding: 101) Select the existing Zhuxiang Chicken ZX line, which meets the weight and appearance standards of 8 and 16 weeks of age, and the hens that meet the weight and appearance standards of 8 and 16 weeks of age, the egg production standard of 43 weeks of age, and the appearance standards, and breed them according to the weight and appearance standards of 8 and 16 weeks of age for roosters, and the weight and appearance standards of 8 and 16 weeks of age for hens, and the egg production standard of 43 weeks of age, and the appearance standards, to obtain the great-grandfather generation ZX paternal line male parent and the great-grandfather generation ZX paternal line female parent; 102) Breeding the great-grandfather ZX paternal line father and the great-grandfather ZX paternal line mother, and selecting and breeding according to the weight standard and appearance standard of roosters at 8 and 16 weeks of age and the weight standard and egg production standard and appearance standard of hens at 8 and 16 weeks of age and 43 weeks of age to obtain the grandparent ZX paternal line father and the grandparent ZX paternal line mother; 103) The grandparent ZX paternal line male parent and the grandparent ZX paternal line female parent are mated and selected according to the weight standard and appearance standard of roosters at 8 and 16 weeks of age, the semen quality standard of roosters at 30 weeks of age, and the positive homozygous genotype of green shell eggs to obtain the parent ZX paternal line male parent; 2) Maternal breeding: 201) Select existing purebred black-bone chicken MW line that meets the weight standard and appearance standard at 8 and 16 weeks of age and the hens that meet the weight standard at 8 and 16 weeks of age and the egg production standard at 43 weeks of age and the appearance standard for breeding, and carry out successive breeding according to the weight standard and appearance standard of the rooster at 8 and 16 weeks of age to obtain the great-grandparent MW maternal father and the grandparent MW maternal mother; 202) Breed the existing purebred Pu'an Panjiang black-bone chicken PA series roosters that meet the weight and appearance standards at 8 and 16 weeks of age and hens that meet the weight standards at 8 and 16 weeks of age and the egg production and appearance standards at 43 weeks of age, and select and breed the great-grandparent PA maternal line females according to the egg production and appearance standards of the hens at 43 weeks of age; 203) The great-grandfather MW maternal sire and the great-grandfather PA maternal sire are mated and selected according to the weight standard and appearance standard of roosters at 8 and 16 weeks of age to obtain the grandparent MP maternal sire; 204) The grandparent MP maternal line male parent and the grandparent MW maternal line female parent were backcrossed and bred according to the weight standards of hens at 8 and 16 weeks of age and the egg production and appearance standards at 43 weeks of age to obtain the parental MM maternal line female parent; 3) The ZX paternal parent obtained by paternal selection and the MM maternal parent obtained by maternal selection can be crossed to obtain the flower-necked green-shell Pu'an Panjiang black-bone chicken.

2. The method for producing seeds of the Pu'an Panjiang black-bone chicken with flower neck and green shell for both meat and eggs as claimed in claim 1, characterized in that: In steps 101) to 103), the selection and breeding of the great-grandfather ZX paternal father, the great-grandfather ZX paternal mother, the grandparent ZX paternal father, the grandparent ZX paternal mother, and the parent ZX paternal father are all carried out by single cage breeding and artificial insemination.

3. The method for producing seeds of the Pu'an Panjiang black-bone chicken with flower neck and green shell for both meat and egg purposes as claimed in claim 1, characterized in that: In steps 201) to 204), the breeding of the great-grandparent MW maternal parent, the great-grandparent PA maternal parent, the grandparent MP maternal parent, the grandparent MW maternal parent, and the parent MM maternal parent are all carried out by hybridization by single cage breeding and artificial insemination.

4. The method for producing seeds of the Pu'an Panjiang black-bone chicken with flower neck and green shell for both meat and egg purposes as claimed in claim 1, characterized in that: In steps 101) to 103), the weight standard of the Zhuxiang chicken rooster at 8 weeks of age is 0.7 kg, the weight standard at 16 weeks of age is 1.75 kg, the egg production standard at 43 weeks of age is that the same family hen lays more than 80 eggs at 43 weeks of age, the appearance characteristics of the Zhuxiang chicken rooster are strong and solid, the feathers have a dark green metallic luster, the sickle feathers are well developed, the feathers, the skin, the legs, the crown and the meat are black, and the semen quality requirements of the Zhuxiang chicken rooster are that the single semen collection volume is more than 0.5 mL, and the milky white The egg production standard of the Zhuxiang chicken hen at 43 weeks of age is 80 eggs or more, the eggshell color is green, and the average egg weight is 50g. The weight standard of the Zhuxiang chicken hen at 8 weeks of age is 0.65kg, and the weight standard at 16 weeks of age is 1.3kg. The appearance characteristics of the Zhuxiang chicken hen are delicate body shape, symmetrical structure, black feathers, black skin, black legs, black crown, and black meat.

5. The method for producing seeds of the Pu'an Panjiang black-bone chicken with flower neck and green shell for both meat and egg purposes as claimed in claim 1, characterized in that: In step 201), the weight standard of the black-bone chicken rooster at 8 weeks of age is 1.5 kg, the weight standard at 16 weeks of age is 2.4 kg, the egg production standard at 43 weeks of age is an average egg production of 85 or more eggs for hens of the same family at 43 weeks of age, and the appearance standard of the black-bone chicken rooster is as follows: a broad and strong physique, a large upright crimson single comb, well-developed sickle feathers, red hemp feathers all over the body, black wing feathers and sickle feathers, black skin, shanks, and meat, and the semen quality requirement of the black-bone chicken rooster is a single semen collection amount. The egg production capacity of the hemp-feathered black-bone chicken is more than 0.7 mL, milky white, thick, with a pH of about 7 to 8, a density of 3 to 5 billion eggs / mL, a vitality of more than 0.7, and a fertilization rate of more than 90%; the weight standard of the hemp-feathered black-bone chicken at 8 weeks of age is 1 kg, the weight standard at 16 weeks of age is 1.6 kg, the egg production standard at 43 weeks of age is more than 85 eggs, and the eggshell color is pink, with an average egg weight of 54 g; the appearance standard of the hemp-feathered black-bone chicken is a wide and round body, a dark red upright single comb, jute feathers all over the body, and black skin, shanks, and meat.

6. The method for producing seeds of the Pu'an Panjiang black-bone chicken with flower neck and green shell for both meat and egg purposes as claimed in claim 1, characterized in that: In step 202), the weight standard of the Pu'an Panjiang black-bone chicken rooster at 8 weeks of age is 0.5 kg, the weight standard at 16 weeks of age is 1.4 kg, the egg production standard at 43 weeks of age is an average egg production of hens of the same family at 43 weeks of age of more than 65 eggs, the appearance standard of the Pu'an Panjiang black-bone chicken rooster is a strong physique, black feathers, black skin, black shanks, black crown, and black meat, and the semen quality requirement of the Pu'an Panjiang black-bone chicken rooster is a single semen collection volume of more than 0.5 mL, milky white , thick, pH is about 7 to 8, density is 3 to 5 billion / mL, vitality is above 0.7, and fertilization rate is above 90%; the weight standard of the Pu'an Panjiang black-bone chicken hen at 8 weeks of age is 0.45kg, the weight standard at 16 weeks of age is 1.2kg, the egg production standard at 43 weeks of age is more than 65 eggs and the eggshell color is pink, the average egg weight is 48g, and the appearance standard of the Pu'an Panjiang black-bone chicken hen is medium size, black feathers, black skin, black legs, black crown, and black meat.

7. The method for producing seeds of the Pu'an Panjiang black-bone chicken with flower neck and green shell for both meat and egg purposes as claimed in claim 1, characterized in that: In step 203), the weight standard of the MP rooster at 8 weeks of age is 1.2 kg, and the weight standard of the MP rooster at 16 weeks of age is 1.9 kg. The appearance standard of the MP rooster is a broad and strong body, a large upright purple-black single comb, red and yellow comb feathers and a small part of the back feathers, black feathers on the wings, abdomen, and tail, black skin, black shanks, and black meat.

8. The method for producing seeds of the Pu'an Panjiang black-bone chicken with flower neck and green shell for both meat and egg purposes as claimed in claim 1, characterized in that: In step 204), the weight standard of the MM hen at 8 weeks of age is 0.8 kg, the weight standard at 16 weeks of age is 1.5 kg, the egg production standard at 43 weeks of age is more than 75 eggs and the eggshell color is pink, and the appearance standard of the MM hen is medium size, purple-black upright single comb, jute neck feathers, chest and part of the back feathers are jute color, wings, abdomen and tail feathers are all black, black skin, black legs and black meat.

9. The method for producing seeds of the Pu'an Panjiang black-bone chicken with flower neck and green shell for both meat and egg purposes as claimed in claim 1, characterized in that: In step 3), the obtained Pu'an Panjiang black-bone chicken with flower neck and green shell has the appearance characteristics of "flower neck, black feathers, black skin, black shanks, black crown and black meat": large body, plump body, wide and deep chest, full abdomen, black upright single crown, overall feathers are black with dark green metallic luster, feathers on the neck are reddish yellow, and the shanks, skin and meat are all black; the obtained Pu'an Panjiang black-bone chicken with flower neck and green shell male and female are put on the market as high-quality broilers at the age of 16 weeks; the hens are sold as local high-quality old hens after the egg production decreases at the age of 43 weeks.

10. The method for producing seeds of the Pu'an Panjiang black-bone chicken with flower neck and green shell for both meat and eggs as claimed in claim 1, characterized in that: Before mating, all breeder chickens in the matching system are purified from Salmonella and chicken leukemia.

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