Earthworm protein polypeptide formula and preparation method thereof

Abstract

The invention discloses an earthworm protein polypeptide formula and a preparation method thereof, and relates to the technical field of biological processing. The traditional Chinese medicine composition comprises lumbricus, silkworm chrysalis, rhodiola rosea, fructus trichosanthis, turmeric, morinda officinalis, walnut kernels, brown sugar, auxiliary sugar, purple perilla, goose carnosine, tremella fuciformis and phellinus igniarius. The preparation method comprises the steps of earthworm hydrolysis, silkworm chrysalis enzymolysis and Chinese herbal medicine extraction and wall breaking, then raw materials are mixed and subjected to two-stage homogenization after secondary shearing, emulsification and filtration, degassing is conducted after filtration is conducted through a ceramic membrane, and then sterile filling is conducted. According to the earthworm oral liquid, microorganism corpses, heavy metals, fishy smell and other unpleasant smells in the earthworm liquid can be effectively removed, it is guaranteed that no Maillard reaction is generated in the filling process of protein, the product is safer, and the effect is more remarkable.

Description

Technical Field

[0001] The invention relates to the technical field of bioprocessing, in particular to a formula of earthworm protein polypeptide and a preparation method thereof. Background Art

[0002] At present, there is no unified quality standard for earth dragons in my country, and earth dragon protein powder is not worthy of its name. It should be more appropriately called earth dragon powder. Let's take Guangdong earth dragon and Shanghai earth dragon as examples. The earth dragon protein powder on the market basically adopts the traditional production process, breaking the earth dragon, washing it with water, drying it in the sun or drying it, freeze-drying it and beating it into powder. This method of producing earth dragon powder can neither preserve the nutritional components of earth dragon protein nor guarantee the product quality, nor can it effectively remove the fishy and sour taste of earth dragon. Some even do not distinguish the growth environment and freshness of the earthworms, and mix them together and wash them directly, allowing them to rot at room temperature, making the earthworm liquid smell fishy and unpleasant. The process equipment is simple and primitive, and not matched. There is no wall breaking and fishy removal equipment in the production process, so small molecule protein wall breaking cannot be carried out. Although the filtration and sterilization process equipment can achieve sterilization effect, it cannot meet the requirements of commercial sterility. There are safety hazards in the product. Due to the influence of earthworm growth conditions, the heavy metal content in earthworm powder exceeds the standard seriously and cannot be controlled at all. Summary of the invention

[0003] The purpose of the present invention is to provide a formula of earthworm protein polypeptide and a preparation method thereof, so as to achieve the purpose that earthworm oral liquid can effectively remove microbial corpses, heavy metals, fishy smell and other bad odors in earthworm liquid, ensure that the protein does not produce Maillard reaction during the filling process, make the product safer and have more significant effects.

[0004] In order to achieve the above object, the present invention adopts the following technical means: A formula of earthworm protein polypeptide comprises earthworm, silkworm pupa, rhodiola rosea, whole trichosanthes, turmeric, Morinda officinalis, walnut kernel, brown sugar, auxiliary sugar, perilla, anserine, tremella, and mulberry linterus.

[0005] Preferably, in parts by weight, the earthworm is 17-48.8 parts, the silkworm pupae are 6-14 parts, the rhodiola rosea is 5-12 parts, the whole trichosanthes is 6-10 parts, the turmeric is 3-6.5 parts, the Morinda officinalis is 5.5-10.5 parts, the walnut kernel is 3-7.8 parts, the brown sugar is 4-5.5 parts, the auxiliary sugar is 5-5.3 parts, the perilla is 2-3 parts, the anserine is 2.5-3.5 parts, the tremella is 1.5-2.5 parts, and the phellin is 2-2.4 parts.

[0006] Furthermore, in parts by weight, the earthworm comprises 40 parts, the silkworm pupae 12 parts, the rhodiola rosea 10 parts, the whole trichosanthes 9 parts, the turmeric 5.3 parts, the Morinda officinalis 9 parts, the walnut kernels 5.4 parts, the brown sugar 5.2 parts, the auxiliary sugar 5.1 parts, the perilla frutescens 2.5 parts, the anserine 3 parts, the tremella fuciformis 2 parts, and the phellinus linteus 2.2 parts.

[0007] Furthermore, the auxiliary sugar is one of honey, xylitol and erythritol.

[0008] In addition, a preparation method of the aforementioned earthworm protein polypeptide formula includes earthworm hydrolysis, silkworm pupa enzymolysis and Chinese herbal medicine extraction and wall breaking, and then the raw materials are mixed and filtered through secondary shear emulsification and then homogenized in two stages, filtered through a ceramic membrane and then degassed, and then aseptically filled.

[0009] Furthermore, in the earthworm hydrolysis step, baking soda is used to neutralize the acid and base to reduce the acidity of the earthworm liquid.

[0010] Furthermore, the homogenization process is carried out at 68-72°C, and high-pressure homogenization is performed first, and then low-pressure homogenization is performed. The high-pressure homogenization is performed at 250kg / cm2.

[0011] Furthermore, the ceramic membrane is a ceramic membrane with a thickness of 0.2 to 2 μm.

[0012] During use, the present invention has the following beneficial effects: In terms of technology, the homogenization wall is broken at an average temperature of 68-72℃ to kill lumbrokinase, so that the amino acids and proteins in silkworm pupae and Chinese medicinal materials form small molecules. Tremella and Chinese medicinal material extracts are added to enhance the effect of protein combination, so that animal protein and plant protein are re-homogenized and combined to become oligopeptides and polypeptides. The use of 0.2-2μm ceramic membrane for sterilization can effectively remove the dead microorganisms, heavy metals, fishy smell and other bad odors in the earthworm liquid, thereby ensuring that the protein does not produce Maillard reaction during the filling process. At the same time, it ensures that the product meets the requirements of commercial sterility, making the product safer and the effect more significant.

[0013] The proportions and types of each formula are formulated according to the pharmacology of monarch, minister, assistant and envoy, and mutual promotion and mutual restraint; in terms of process flow, edible baking soda is used for acid-base neutralization to reduce the acidity of earth dragon liquid, and the ceramic membrane is used for filtration and sterilization, and the degassing device is used to remove the fishy smell of earth dragon liquid. More than 98~99.9% of microorganisms are filtered out, the pH value is controlled at 5.6~7.0, the solid content reaches more than 9.8~12%, the fat is controlled at 0.1~0.4%, and the protein content reaches more than 3.6~4.2%. DETAILED DESCRIPTION

[0014] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments.

[0015] Therefore, the following detailed description of the embodiments of the present invention is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0016] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features of the embodiments may be combined with each other. Example

[0017] A formula of earthworm protein polypeptide comprises earthworm, silkworm pupa, rhodiola rosea, whole trichosanthes, turmeric, Morinda officinalis, walnut kernel, brown sugar, auxiliary sugar, perilla, anserine, tremella, and mulberry linterus.

[0018] In parts by weight, the earthworm comprises 17 parts, the silkworm pupae comprise 6 parts, the rhodiola rosea comprise 5 parts, the whole trichosanthes comprise 6 parts, the turmeric comprise 3 parts, the Morinda officinalis comprise 5.5 parts, the walnut kernel comprise 3 parts, the brown sugar comprise 4 parts, the auxiliary sugar comprise 5 parts, the perilla comprise 2 parts, the anserine comprise 2.5 parts, the tremella comprise 1.5 parts, and the phellin comprise 2 parts.

[0019] The auxiliary sugar is one of honey, xylitol and erythritol.

[0020] In addition, a preparation method of the aforementioned earthworm protein polypeptide formula includes earthworm hydrolysis, silkworm pupa enzymolysis and Chinese herbal medicine extraction and wall breaking, and then the raw materials are mixed and filtered through secondary shear emulsification and then homogenized in two stages, filtered through a ceramic membrane and then degassed, and then aseptically filled.

[0021] Furthermore, in the earthworm hydrolysis step, baking soda is used to neutralize the acid and base to reduce the acidity of the earthworm liquid.

[0022] Furthermore, the homogenization process is carried out at 68-72°C, and high-pressure homogenization is performed first, and then low-pressure homogenization is performed. The high-pressure homogenization is performed at 250kg / cm2.

[0023] Furthermore, the ceramic membrane is a ceramic membrane with a thickness of 0.2 to 2 μm. Example

[0024] A formula of earthworm protein polypeptide comprises earthworm, silkworm pupa, rhodiola rosea, whole trichosanthes, turmeric, Morinda officinalis, walnut kernel, brown sugar, auxiliary sugar, perilla, anserine, tremella, and mulberry linterus.

[0025] In parts by weight, the ingredients include 48.8 parts of earthworm, 14 parts of silkworm pupae, 12 parts of rhodiola rosea, 10 parts of whole trichosanthes, 6.5 parts of turmeric, 10.5 parts of Morinda officinalis, 7.8 parts of walnut kernels, 5.5 parts of brown sugar, 5.3 parts of auxiliary sugar, 3 parts of perilla, 3.5 parts of anserine, 2.5 parts of tremella, and 2.4 parts of mulberry linterus.

[0026] The auxiliary sugar is one of honey, xylitol and erythritol.

[0027] In addition, a preparation method of the aforementioned earthworm protein polypeptide formula includes earthworm hydrolysis, silkworm pupa enzymolysis and Chinese herbal medicine extraction and wall breaking, and then the raw materials are mixed and filtered through secondary shear emulsification and then homogenized in two stages, filtered through a ceramic membrane and then degassed, and then aseptically filled.

[0028] Furthermore, in the earthworm hydrolysis step, baking soda is used to neutralize the acid and base to reduce the acidity of the earthworm liquid.

[0029] Furthermore, the homogenization process is carried out at 68-72°C, and high-pressure homogenization is performed first, and then low-pressure homogenization is performed. The high-pressure homogenization is performed at 250kg / cm2.

[0030] Furthermore, the ceramic membrane is a ceramic membrane with a thickness of 0.2 to 2 μm. Example

[0031] A formula of earthworm protein polypeptide comprises earthworm, silkworm pupa, rhodiola rosea, whole trichosanthes, turmeric, Morinda officinalis, walnut kernel, brown sugar, auxiliary sugar, perilla, anserine, tremella, and mulberry linterus.

[0032] In parts by weight, the ingredients include 40 parts of earthworm, 12 parts of silkworm pupae, 10 parts of rhodiola rosea, 9 parts of whole trichosanthes, 5.3 parts of turmeric, 9 parts of Morinda officinalis, 5.4 parts of walnut kernels, 5.2 parts of brown sugar, 5.1 parts of auxiliary sugar, 2.5 parts of perilla, 3 parts of anserine, 2 parts of tremella, and 2.2 parts of mulberry linterus.

[0033] The auxiliary sugar is one of honey, xylitol and erythritol.

[0034] In addition, a preparation method of the aforementioned earthworm protein polypeptide formula includes earthworm hydrolysis, silkworm pupa enzymolysis and Chinese herbal medicine extraction and wall breaking, and then the raw materials are mixed and filtered through secondary shear emulsification and then homogenized in two stages, filtered through a ceramic membrane and then degassed, and then aseptically filled.

[0035] Furthermore, in the earthworm hydrolysis step, baking soda is used to neutralize the acid and base to reduce the acidity of the earthworm liquid.

[0036] Furthermore, the homogenization process is carried out at 68-72°C, and high-pressure homogenization is performed first, and then low-pressure homogenization is performed. The high-pressure homogenization is performed at 250kg / cm2.

[0037] Furthermore, the ceramic membrane is a ceramic membrane with a thickness of 0.2 to 2 μm.

[0038] Based on the configuration of the above embodiments, the earthworm and silkworm pupa of the present invention are respectively hydrolyzed and enzymolyzed, and then bio-fermented; and the wall is broken with other ingredients for homogenization, so that the plant egg and the animal protein are organically combined, and the amino acids reach more than fifty, so that the amino acids of the product are transformed from oligopeptides to polypeptides with higher human absorption rate and stronger product functionality. If a single earthworm is used, without the use of enzyme engineering bio-fermentation technology and wall-breaking technology, it is impossible to break the wall of the protein and amino acids in the product to generate oligopeptides, let alone polypeptides.

[0039] Moreover, during the homogenization process, if high-pressure homogenization is not used to break the wall, the product cannot form small molecule peptides, and the product cannot be transformed from oligopeptides to polypeptides. In terms of technology, physical ceramic membrane filtration sterilization and high-pressure homogenization are used to break the protein wall and further refine it into small molecules. The crude protein in the raw material can be broken from one protein into 1,200 small molecule proteins, making it taste smoother and more delicate. Under the action of the homogenizer, plant protein and animal protein are organically combined and linked again, so that the small molecule peptides form a connected state. After completing the high-pressure homogenization, low-pressure homogenization is used to link the broken small protein and Tremella collagen polysaccharide, so that the animal protein and plant protein form a new organism under the adhesion of Tremella collagen, and the product has been greatly improved in function and sensory perception.

[0040] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A formula of earthworm protein polypeptide, characterized in that: Including earthworms, silkworm pupae, rhodiola rosea, whole trichosanthes, turmeric, Morinda officinalis, walnut kernels, brown sugar, auxiliary sugar, perilla, anserine, tremella, and mulberry linterus.

2. A formula of earthworm protein polypeptide according to claim 1, characterized in that: In parts by weight, the earthworm comprises 17 to 48.8 parts, the silkworm pupae comprise 6 to 14 parts, the rhodiola rosea comprise 5 to 12 parts, the whole trichosanthes comprise 6 to 10 parts, the turmeric comprise 3 to 6.5 parts, the Morinda officinalis comprise 5.5 to 10.5 parts, the walnut kernel comprise 3 to 7.8 parts, the brown sugar comprise 4 to 5.5 parts, the auxiliary sugar comprise 5 to 5.3 parts, the perilla comprise 2 to 3 parts, the anserine comprise 2.5 to 3.5 parts, the tremella comprise 1.5 to 2.5 parts, and the phellin comprise 2 to 2.4 parts.

3. A formula of earthworm protein polypeptide according to claim 2, characterized in that: In parts by weight, the ingredients include 40 parts of earthworm, 12 parts of silkworm pupae, 10 parts of rhodiola rosea, 9 parts of whole trichosanthes, 5.3 parts of turmeric, 9 parts of Morinda officinalis, 5.4 parts of walnut kernels, 5.2 parts of brown sugar, 5.1 parts of auxiliary sugar, 2.5 parts of perilla, 3 parts of anserine, 2 parts of tremella, and 2.2 parts of mulberry linterus.

4. A formula of earthworm protein polypeptide according to any one of claims 1 to 3, characterized in that: The auxiliary sugar is one of honey, xylitol and erythritol.

5. A method for preparing the earthworm protein polypeptide formula according to any one of claims 1 to 4, characterized in that: It includes hydrolysis of earthworms, enzymatic hydrolysis of silkworm pupae and wall breaking of Chinese herbal medicines. The raw materials are then mixed and filtered through secondary shear emulsification before being homogenized in two stages. The mixture is then filtered through a ceramic membrane for degassing before being aseptically filled.

6. The preparation method according to claim 5, characterized in that: In the earthworm hydrolysis step, baking soda is used to neutralize the acid and alkali to reduce the acidity of the earthworm liquid.

7. The preparation method according to claim 5, characterized in that: The homogenization process is carried out at 68-72°C, and high-pressure homogenization is performed first, and then low-pressure homogenization is performed. The high-pressure homogenization is performed at 250kg / cm2.

8. The preparation method according to claim 5, characterized in that: The ceramic membrane is a ceramic membrane with a thickness of 0.2 to 2 μm.