Composition with function of accelerating lipolysis and preparation method thereof

Abstract

The invention relates to the technical field of health care products, in particular to a composition with a function of accelerating lipolysis and a preparation method of the composition. Comprising the following steps: preparing silkworm chrysalis polypeptide; preparing a silkworm chrysalis polypeptide-turnip flower extract compound; preparing morinda citrifolia-opuntia ficus-indica composite ferment powder; and compounding and mixing the extract. The morinda citrifolia-opuntia ficus-indica composite ferment powder, the silkworm chrysalis polypeptide-turnip flower extract compound, the syzygium samarangense extract, the laver extract and the inulin are compounded, so that the effect of synergistically accelerating decomposition of fat in a body can be achieved, aggregation of the fat in the body is effectively reduced, and a good effect of inhibiting obesity is achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of health care products, and in particular to a composition having the function of accelerating fat decomposition and a preparation method thereof. Background Art

[0002] Obesity, as a global health problem, is spreading at a rapid rate and has become an important risk factor for metabolic diseases such as diabetes, cardiovascular disease, and hyperlipidemia. Its main cause is closely related to unhealthy eating habits and lifestyles, especially long-term excessive intake of high-fat diets, which leads to excessive accumulation of body fat, especially triglycerides. It is a multifactorial chronic metabolic disease.

[0003] Currently, the commonly used weight loss and lipid-lowering drugs in clinical practice, such as orlistat, liraglutide and statins, play a role in reducing obesity to a certain extent by inhibiting fat absorption and improving lipid metabolism. However, these drugs are often accompanied by significant adverse reactions, such as gastrointestinal discomfort, oily stools and fecal urgency, which affect the quality of life of patients. Therefore, it is of great significance and challenging to develop weight loss products that are natural, highly effective, non-toxic and have no side effects, can improve lipid metabolism and accelerate the decomposition of fat in the body. Summary of the invention

[0004] In order to solve the above technical defects, the present invention has developed a composition with the function of accelerating fat decomposition and a preparation method thereof, which rationally compounded the components that can improve lipogenesis, can synergistically accelerate the decomposition of fat in the body, and has a good effect on inhibiting obesity.

[0005] A method for preparing a composition having the function of accelerating fat decomposition comprises the following steps: S1: Preparation of silkworm pupa polypeptides The silkworm pupae are homogenized, freeze-dried, crushed and ethanol-extracted, and the precipitate is collected and dried, and then water is added to the homogenate for enzymatic hydrolysis, and the enzyme is inactivated and then centrifuged to obtain the supernatant, which is freeze-dried to obtain the silkworm pupae polypeptide; S2: Preparation of silkworm pupa polypeptide-turnip flower extract compound The whole turnip flower is washed and crushed, and then added to a solution mixed with choline chloride, glycerol and DL-tartaric acid at high temperature, and then ultrasonically extracted and centrifuged to obtain the supernatant, and succinic anhydride and activated 4A molecular sieve are added to react, and then dried and mixed with silkworm pupa polypeptide and water, and freeze-dried and crushed to obtain a silkworm pupa polypeptide-turnip flower extract composite; S3: Preparation of Noni-Prickly Pear Cactus Composite Fermentation Powder and Compounding of Extracts Add water to the noni fruit and boil it, then add the prickly pear cactus extract, white sugar and pectinase, inoculate Pichia kluyveri for fermentation, centrifuge to collect the supernatant, freeze-dry, and grind to obtain the noni fruit-prickly pear cactus composite fermentation powder, mix it evenly with the silkworm pupa polypeptide-turnip flower extract compound, inulin and stachyose, dry and grind it, then add the wax apple extract, laver extract and resistant dextrin and mix evenly to obtain a composition with the function of accelerating fat decomposition.

[0006] Furthermore, step S1, the preparation of silkworm pupa polypeptide, comprises the following steps: S1.1: Take 4-5 portions of silkworm pupae and wash them clean, then add water to the mixture at a solid-liquid ratio of 1:(2-3) g / mL to make a homogenate for freeze-drying, crush and pass through a 30-80 mesh sieve to obtain silkworm pupa powder, mix the silkworm pupa powder with a 90-95% ethanol solution at a solid-liquid ratio of 1:(12-15) g / mL for extraction, the extraction temperature is 30-35°C, the extraction time is 8-10 hours, then centrifuge and filter, collect the precipitate and dry it, and obtain defatted silkworm pupa powder; S1.2: Add water to the defatted silkworm pupa powder at a solid-liquid ratio of 1: (3-4) g / mL to homogenize, adjust the pH to 4-5, first add 2-3% of the weight of cellulase to the defatted silkworm pupa powder, hydrolyze at 50-55°C for 4-6 hours, inactivate the enzyme in a boiling water bath for 10-12 minutes, then cool to 50-55°C, adjust the pH to 8.5-9, then add 3-4% of the weight of protease to the defatted silkworm pupa powder, hydrolyze for 6-8 hours to obtain an enzymolysis solution, inactivate the enzyme in a boiling water bath for 10-12 minutes, quickly cool to room temperature, centrifuge at 8000-10000 rpm at 3-4°C for 12-15 minutes, collect the supernatant and freeze-dry to obtain silkworm pupa polypeptides.

[0007] Furthermore, step S2, the preparation of the silkworm pupa polypeptide-turnip flower extract composite comprises the following steps: S2.1: Wash and dry the whole turnip flower, and then crush it to obtain turnip flower powder, mix choline chloride, glycerol and DL-tartaric acid in a molar ratio of 1: (2-3): (0.5-0.6) in a container, stir continuously at 100-105° C. for 25-30 minutes, cool to room temperature, add 4-6wt% turnip flower powder, and then transfer to an ultrasonic extractor for ultrasonic extraction, and then centrifuge at a speed of 4000-5000rpm for 6-8 minutes in a centrifuge, take the supernatant and filter through a filter membrane with a pore size of 0.2-0.5um to obtain a turnip flower extract; S2.2: Add 1-2wt% of succinic anhydride and 1.5-3wt% of activated 4A molecular sieve to the turnip flower extract, mix them evenly with an ultrasonic extractor at a power of 100-150W, heat them in a water bath to carry out esterification modification reaction, filter them after the reaction to remove succinic anhydride and activated 4A molecular sieve, and vacuum dry them to obtain dry powder; S2.3: Place the dry powder and silkworm pupa polypeptide in a container at a mass ratio of 1: (2-3), add 80-100 times the mass of ultrapure water, then stir at 25-30°C at a stirring speed of 800-1000rpm for 2-2.5 hours, freeze-dry at -50°C--40°C, and then crush to obtain a silkworm pupa polypeptide-turnip flower extract complex.

[0008] Furthermore, step S3 of preparing the Noni fruit-Opuntia cactus composite fermentation powder and compounding and mixing the extract comprises the following steps: S3.1: Take 2-3 parts of prickly pear cactus, remove the hair and thorns on the surface, then rinse, cut and crush, add 3-5 times the weight of water and boil for 2-3 hours, filter to obtain an extract, repeat 1-2 times to combine the extracts to obtain a prickly pear cactus extract, take 3-4 parts of fresh and ripe noni fruit, clean and crush, add 3-5 times the weight of water and boil for 1.5-2 hours to obtain noni pulp, mix the prickly pear cactus extract and noni pulp, add 10-12wt% of white sugar and 1-2wt% of pectinase, and then inoculate Pichia kluyveri at an inoculum amount of 3-5% for fermentation to obtain a fermentation material; S3.2: Filter the fermented material with a mesh screen to obtain fermentation filtrate I, centrifuge the fermentation filtrate I in a centrifuge at a speed of 5000-6000 rpm for 15-20 minutes, filter the supernatant through a filter membrane to obtain fermentation filtrate II, freeze-dry the fermentation filtrate II at minus 70°C to minus 50°C, and crush to obtain noni fruit-pear cactus composite fermentation powder; S3.3: Mix 10-15 parts of noni fruit-prickly pear cactus composite fermentation powder, 8-10 parts of silkworm pupa polypeptide-turnip flower extract compound, 2-3 parts of inulin and 0.5-1 part of stachyose, dry and crush, add 3-5 parts of wax apple extract, 2-4 parts of laver extract and 10-15 parts of resistant dextrin and mix evenly to obtain a composition with the function of accelerating fat decomposition.

[0009] Furthermore, the protease in step S1.2 is alkaline protease.

[0010] Furthermore, in step S2.1, the temperature of ultrasonic extraction is 45-50°C, the ultrasonic power is 150-200W, the ultrasonic frequency is 25-30kHz, and the extraction time is 30-50 minutes.

[0011] Furthermore, in step S2.2, the water bath heating temperature is 60-65° C., and the water bath time is 4-5 hours.

[0012] Furthermore, the fermentation temperature in step S3.1 is 25-30° C., and the fermentation time is 8-10 days.

[0013] Furthermore, the pore size of the filter membrane in step S3.2 is 0.8-1 um.

[0014] A composition with the function of accelerating fat decomposition is prepared by the method for preparing the composition with the function of accelerating fat decomposition.

[0015] The beneficial effects are: 1. The present invention can synergistically accelerate the decomposition of fat in the body, effectively reduce the accumulation of fat in the body, and has a good effect on inhibiting obesity by compounding noni fruit-pear cactus composite fermentation powder, silkworm pupa polypeptide-turnip flower extract compound, wax apple extract, laver extract and inulin.

[0016] 2. The present invention places silkworm pupa polypeptide and turnip flower extract dry powder in ultrapure water, stirs for 2-2.5 hours at 25-30° C., freeze-dries and then crushes to obtain a silkworm pupa polypeptide-turnip flower extract composite. The amino acid residues in the silkworm pupa polypeptide can interact with the flavonoid compounds in the turnip flower to assemble into a structure that protects the activity of both, thereby improving the digestion stability of the two, promoting the active ingredients to exert their effects, inhibiting the activity of pancreatic lipase to reduce the digestion and absorption of fat, and synergistically enhancing the ability to decompose subcutaneous fat and visceral fat.

[0017] 3. The present invention adds succinic anhydride and activated 4A molecular sieves to the turnip flower extract, and the flavonoids and polyphenols undergo esterification reaction with the acylating agent succinic anhydride. At the same time, the 4A molecular sieve adsorbs the generated water to positively promote the reaction and improve the esterification efficiency. This can not only enhance the stability of the active ingredients in the turnip flower extract, but also improve its ability to decompose fat. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a flow chart of the preparation method of the composition with the function of accelerating fat decomposition adopted in the embodiments of the present invention. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] Example 1: A composition having the function of accelerating fat decomposition and a preparation method thereof, such as Figure 1 As shown, the following steps are included: S1: Preparation of silkworm pupa polypeptides S1.1: Take 4 portions of silkworm pupa water and wash them clean, then add water to the mixture at a solid-liquid ratio of 1:2 g / mL and slurry them for freeze drying, crush them through a 30-mesh sieve to obtain silkworm pupa powder, mix the silkworm pupa powder with a 90% ethanol solution at a solid-liquid ratio of 1:12 g / mL and extract them, the extraction temperature is 30°C, the extraction time is 8 hours, then centrifuge and filter, collect the precipitate and dry it, and obtain defatted silkworm pupa powder; S1.2: Add water to the defatted silkworm pupa powder at a solid-liquid ratio of 1:3 g / mL and homogenize, adjust the pH to 4, first add 2% of the weight of cellulase in the defatted silkworm pupa powder, hydrolyze at 50°C for 4 hours, inactivate the enzyme in a boiling water bath for 10 minutes, then cool to 50°C, adjust the pH to 8.5, then add 3% of the weight of alkaline protease in the defatted silkworm pupa powder, hydrolyze for 6 hours to obtain an enzymolysis solution, inactivate the enzyme in a boiling water bath for 10 minutes, quickly cool to room temperature, centrifuge at 8000 rpm at 3°C ​​for 12 minutes, collect the supernatant and freeze-dry to obtain silkworm pupa polypeptides.

[0021] S2: Preparation of silkworm pupa polypeptide-turnip flower extract compound S2.1: Wash and dry the whole turnip flower, and then crush it to obtain turnip flower powder. Mix choline chloride, glycerol and DL-tartaric acid in a molar ratio of 1:2:0.5 in a container, and continue stirring at 100°C for 25 minutes. After cooling to room temperature, add 4wt% turnip flower powder, and then transfer to an ultrasonic extractor for ultrasonic extraction. The ultrasonic extraction temperature is 45°C, the ultrasonic power is 150W, and the ultrasonic frequency is 25kHz. Extract for 30 minutes, then place it in a centrifuge and centrifuge it at a speed of 4000rpm for 6 minutes. Take the supernatant and filter it through a filter membrane with a pore size of 0.2um to obtain a turnip flower extract; S2.2: Add 1wt% succinic anhydride and 1.5wt% activated 4A molecular sieve to the turnip flower extract, mix them evenly with an ultrasonic extractor at a power of 100W, heat them in a water bath for esterification modification, the water bath heating temperature is 60°C, the water bath time is 4 hours, filter after the reaction to remove succinic anhydride and activated 4A molecular sieve, and obtain dry powder after vacuum drying; S2.3: Place the dry powder and silkworm pupa polypeptide in a container at a mass ratio of 1:2, add 80 times the mass of ultrapure water, then stir at 800 rpm at 25°C for 2 hours, freeze-dry at minus 50°C and crush to obtain a silkworm pupa polypeptide-turnip flower extract complex.

[0022] S3: Preparation of Noni-Prickly Pear Cactus Composite Fermentation Powder and Compounding of Extracts S3.1: Take 2 parts of prickly pear cactus, remove the hair and thorns on the surface, then rinse, cut and crush, add 3 times the weight of water and boil for 2 hours, filter to obtain an extract, repeat once to combine the extracts to obtain a prickly pear cactus extract, take 3 parts of fresh and ripe noni fruit, wash and crush, add 3 times the weight of water and boil for 1.5 hours to obtain noni pulp, mix the prickly pear cactus extract and noni pulp, add 10wt% of white sugar and 1wt% of pectinase, and then inoculate Pichia kluyveri at an inoculation amount of 3% for fermentation, the fermentation temperature is 25°C, and the fermentation time is 8 days to obtain a fermented material; S3.2: Filter the fermented material with a mesh screen to obtain fermentation filtrate I, centrifuge the fermentation filtrate I in a centrifuge at a speed of 5000 rpm for 15 minutes, filter the supernatant through a filter membrane with a pore size of 0.8 um to obtain fermentation filtrate II, freeze-dry the fermentation filtrate II at minus 70° C., and crush to obtain noni fruit-pear cactus composite fermentation powder; S3.3: Mix 10 parts of noni fruit-prickly pear cactus composite fermentation powder, 8 parts of silkworm pupa polypeptide-turnip flower extract compound, 2 parts of inulin and 0.5 parts of stachyose, dry and crush, add 3 parts of wax apple extract, 2 parts of laver extract and 10 parts of resistant dextrin and mix evenly to obtain a composition with the function of accelerating fat decomposition.

[0023] Example 2: A composition having the function of accelerating fat decomposition and a preparation method thereof, such as Figure 1 As shown, the following steps are included: S1: Preparation of silkworm pupa polypeptides S1.1: Take 5 portions of silkworm pupa water, wash them clean, then add water to the mixture at a solid-liquid ratio of 1:2 g / mL, homogenize them, freeze-dry them, crush them through a 30-mesh sieve to obtain silkworm pupa powder, mix the silkworm pupa powder with a 90% ethanol solution at a solid-liquid ratio of 1:12 g / mL, extract them at a temperature of 30°C for 8 hours, then centrifuge and filter them, collect the precipitate and dry them, and obtain defatted silkworm pupa powder; S1.2: Add water to the defatted silkworm pupa powder at a solid-liquid ratio of 1:3 g / mL and homogenize, adjust the pH to 4, first add 3% of the weight of cellulase in the defatted silkworm pupa powder, hydrolyze at 50°C for 4 hours, inactivate the enzyme in a boiling water bath for 10 minutes, then cool to 50°C and adjust the pH to 8.5, then add 3% of the weight of alkaline protease in the defatted silkworm pupa powder, hydrolyze for 6 hours to obtain an enzymolysis solution, inactivate the enzyme in a boiling water bath for 10 minutes, quickly cool to room temperature, centrifuge at 8000 rpm at 3°C ​​for 12 minutes, collect the supernatant and freeze-dry to obtain silkworm pupa polypeptides.

[0024] S2: Preparation of silkworm pupa polypeptide-turnip flower extract compound S2.1: Wash and dry the whole turnip flower, and then crush it to obtain turnip flower powder. Mix choline chloride, glycerol and DL-tartaric acid in a molar ratio of 1:3:0.6 in a container, and continue stirring at 100°C for 25 minutes. After cooling to room temperature, add 6wt% turnip flower powder, and then transfer to an ultrasonic extractor for ultrasonic extraction. The ultrasonic extraction temperature is 45°C, the ultrasonic power is 150W, and the ultrasonic frequency is 25kHz. Extract for 30 minutes, then place it in a centrifuge and centrifuge it at a speed of 4000rpm for 6 minutes, take the supernatant and filter it through a filter membrane with a pore size of 0.2um to obtain a turnip flower extract; S2.2: Add 2wt% of succinic anhydride and 3wt% of activated 4A molecular sieve to the turnip flower extract, mix them evenly with an ultrasonic extractor at a power of 100W, heat them in a water bath for esterification modification, the water bath heating temperature is 60°C, the water bath time is 4 hours, filter after the reaction is completed to remove succinic anhydride and activated 4A molecular sieve, and obtain dry powder after vacuum drying; S2.3: Place the dry powder and silkworm pupa polypeptide in a container at a mass ratio of 1:3, add 100 times the mass of ultrapure water, then stir at 800 rpm at 25°C for 2 hours, freeze-dry at minus 50°C and crush to obtain a silkworm pupa polypeptide-turnip flower extract complex.

[0025] S3: Preparation of Noni-Prickly Pear Cactus Composite Fermentation Powder and Compounding of Extracts S3.1: Take 3 parts of prickly pear cactus, remove the hair and thorns on the surface, then rinse, cut and crush, add 3 times the weight of water and boil for 2 hours, filter to obtain an extract, repeat once to combine the extracts to obtain a prickly pear cactus extract, take 4 parts of fresh and ripe noni fruit, wash and crush, add 3 times the weight of water and boil for 1.5 hours to obtain noni pulp, mix the prickly pear cactus extract and noni pulp, add 12wt% of white sugar and 2wt% of pectinase, and then inoculate Pichia kluyveri at an inoculum amount of 5% for fermentation, the fermentation temperature is 25°C, and the fermentation time is 8 days to obtain a fermented material; S3.2: Filter the fermented material with a mesh screen to obtain fermentation filtrate I, centrifuge the fermentation filtrate I in a centrifuge at a speed of 5000 rpm for 15 minutes, filter the supernatant through a filter membrane with a pore size of 0.8 um to obtain fermentation filtrate II, freeze-dry the fermentation filtrate II at minus 70° C., and crush to obtain noni fruit-pear cactus composite fermentation powder; S3.3: Mix 15 parts of noni fruit-prickly pear cactus composite fermentation powder, 10 parts of silkworm pupa polypeptide-turnip flower extract compound, 3 parts of inulin and 1 part of stachyose, dry and crush, add 5 parts of wax apple extract, 4 parts of laver extract and 15 parts of resistant dextrin and mix evenly to obtain a composition with the function of accelerating fat decomposition.

[0026] Example 3: A composition having the function of accelerating fat decomposition and a preparation method thereof, such as Figure 1 As shown, the following steps are included: S1: Preparation of silkworm pupa polypeptides S1.1: Take 4 portions of silkworm pupa water and wash them clean, then add water to the mixture at a solid-liquid ratio of 1:3 g / mL for homogenization and freeze-drying, crush and pass through an 80-mesh sieve to obtain silkworm pupa powder, mix the silkworm pupa powder with a 95% ethanol solution at a solid-liquid ratio of 1:15 g / mL for extraction, the extraction temperature is 35°C, the extraction time is 10 hours, then centrifuge and filter, collect the precipitate and dry it, and obtain defatted silkworm pupa powder; S1.2: Add water to the defatted silkworm pupa powder at a solid-liquid ratio of 1:4 g / mL and homogenize, adjust the pH to 5, first add 2% of the weight of cellulase in the defatted silkworm pupa powder, hydrolyze at 55°C for 6 hours, inactivate the enzyme in a boiling water bath for 12 minutes, then cool to 55°C, adjust the pH to 9, then add 3% of the weight of alkaline protease in the defatted silkworm pupa powder, hydrolyze for 8 hours to obtain an enzymolysis solution, inactivate the enzyme in a boiling water bath for 12 minutes, quickly cool to room temperature, centrifuge at 10,000 rpm at 4°C for 15 minutes, collect the supernatant and freeze-dry to obtain silkworm pupa polypeptides.

[0027] S2: Preparation of silkworm pupa polypeptide-turnip flower extract compound S2.1: Wash and dry the whole turnip flower, and then crush it to obtain turnip flower powder. Mix choline chloride, glycerol and DL-tartaric acid in a molar ratio of 1:2:0.5 in a container, and continue stirring at 105°C for 30 minutes. After cooling to room temperature, add 4wt% turnip flower powder, and then transfer to an ultrasonic extractor for ultrasonic extraction. The ultrasonic extraction temperature is 50°C, the ultrasonic power is 200W, and the ultrasonic frequency is 30kHz. Extract for 50 minutes, then place in a centrifuge and centrifuge at a speed of 5000rpm for 8 minutes, take the supernatant and filter through a 0.5um pore size filter membrane to obtain a turnip flower extract; S2.2: Add 1wt% succinic anhydride and 1.5wt% activated 4A molecular sieve to the turnip flower extract, use an ultrasonic extractor to mix evenly at a power of 150W, heat in a water bath for esterification modification reaction, the water bath heating temperature is 65°C, the water bath time is 5 hours, filter after the reaction is completed to remove succinic anhydride and activated 4A molecular sieve, and obtain dry powder after vacuum drying; S2.3: Place the dry powder and silkworm pupa polypeptide in a container at a mass ratio of 1:2, add 80 times the mass of ultrapure water, then stir at 30°C at a stirring speed of 1000 rpm for 2.5 hours, freeze-dry at minus 40°C and crush to obtain a silkworm pupa polypeptide-turnip flower extract complex.

[0028] S3: Preparation of Noni-Prickly Pear Cactus Composite Fermentation Powder and Compounding of Extracts S3.1: Take 2 parts of prickly pear cactus, remove the hair and thorns on the surface, then rinse, cut and crush, add 3 times the weight of water and boil for 3 hours, filter to obtain an extract, repeat 2 times to combine the extracts to obtain a prickly pear cactus extract, take 4 parts of fresh and ripe noni fruit, wash and crush, add 3 times the weight of water and boil for 2 hours to obtain noni pulp, mix the prickly pear cactus extract and noni pulp, add 10wt% of white sugar and 1wt% of pectinase, and then inoculate Pichia kluyveri at an inoculum amount of 3% for fermentation, the fermentation temperature is 30°C, and the fermentation time is 10 days to obtain a fermented material; S3.2: Filter the fermented material with a mesh screen to obtain fermentation filtrate I, centrifuge the fermentation filtrate I in a centrifuge at a speed of 6000 rpm for 20 minutes, filter the supernatant through a filter membrane with a pore size of 1 um to obtain fermentation filtrate II, freeze-dry the fermentation filtrate II at minus 50° C., and crush to obtain noni fruit-pear cactus composite fermentation powder; S3.3: Mix 10 parts of noni fruit-prickly pear cactus composite fermentation powder, 8 parts of silkworm pupa polypeptide-turnip flower extract compound, 2 parts of inulin and 0.5 parts of stachyose, dry and crush, add 3 parts of wax apple extract, 2 parts of laver extract and 10 parts of resistant dextrin and mix evenly to obtain a composition with the function of accelerating fat decomposition.

[0029] Comparative Example 1: The difference from Example 1 is that in Comparative Example 1, the silkworm pupa polypeptide-turnip flower extract compound is not added in step S3.2, and the remaining steps are the same as those in Example 1, and a composition with the function of accelerating fat decomposition is prepared, which is recorded as Comparative Example 1.

[0030] Comparative Example 2: The difference from Example 1 is that in Comparative Example 2, no Noni fruit-Opuntia cactus composite fermentation powder is added in step S3.2, and the remaining steps are the same as those in Example 1 to prepare a composition with the function of accelerating fat decomposition, which is recorded as Comparative Example 2.

[0031] Comparative Example 3: The difference from Example 1 is that in Comparative Example 3, wax apple extract is not added in step S3.2, and the remaining steps are the same as those in Example 1, to obtain a composition having the function of accelerating fat decomposition, which is recorded as Comparative Example 3.

[0032] Comparative Example 4: The difference from Example 1 is that in Comparative Example 4, no laver extract is added in step S3.2, and the remaining steps are the same as those in Example 1, to obtain a composition having the function of accelerating fat decomposition, which is recorded as Comparative Example 4.

[0033] Comparative Example 5: The difference from Example 1 is that, in Comparative Example 5, step S2.3 is removed, and the silkworm pupa polypeptide-turnip flower extract complex in step S3.2 is replaced with dry powder and silkworm pupa polypeptide in an equal mass ratio of 1:2. The remaining steps are the same as in Example 1, and a composition with the function of accelerating fat decomposition is obtained, which is recorded as Comparative Example 5.

[0034] Comparative Example 6: The difference from Example 1 is that in Example 6, step S2.2 is removed, and the turnip flower extract is directly vacuum-dried to obtain a dry powder. The remaining steps are the same as those in Example 1, and a composition with the function of accelerating fat decomposition is obtained, which is recorded as Comparative Example 6.

[0035] Experiment 1: Take the same mass of the composition with the function of accelerating fat decomposition prepared in Examples 1-3 and Comparative Examples 1-6, and prepare a 70 mg / mL sample solution with anhydrous ethanol, take 0.2 mL of the sample solution into a test tube, add 0.5 mL of a 1.2 mg / mL pancreatic lipase solution and 1.2 mL of a 0.1 mol / L Tris-HCl buffer solution in sequence, place in a water bath at 37°C and heat for 15 minutes, then add 1 mL of a 4-nitrophenyl palmitate solution (4-nitrophenyl palmitate, anhydrous ethanol) and stir until the mixture is stirred. , Tris-HCl buffer, concentration of 0.2mmol / L) and shake well, continue to keep in 37℃ water bath for 20 minutes, measure the absorbance at 405nm and record it as A1, replace the sample solution with Tris-HCl buffer and carry out the above steps to measure the absorbance and record it as A2, add 4-nitrophenyl palmitate solution and react for 0 minutes to measure the absorbance and record it as A0, calculate the pancreatic lipase inhibition rate = (A2-A1) / (A2-A0)×100%, repeat two parallel experiments, record the data and make a table, as shown in Table 1.

[0036] Table 1: Inhibition rate of pancreatic lipase by the composition with the function of accelerating fat decomposition

[0037] It can be seen from the data of Example 1 and Comparative Examples 1-4 in Table 1 that compounding the noni fruit-pear cactus composite fermentation powder, silkworm pupa polypeptide-turnip flower extract compound, wax apple extract, laver extract and inulin can synergistically inhibit pancreatic lipase in the body and has a good effect on inhibiting obesity.

[0038] It can be seen from the data of Example 1 and Comparative Examples 5-6 in Table 1 that the silkworm pupa polypeptide and turnip flower extract dry powder are used to prepare a silkworm pupa polypeptide-turnip flower extract composite, which improves the digestion stability of the two, promotes the function of the active ingredients, and can inhibit the activity of pancreatic lipase to reduce the digestion and absorption of fat. In addition, succinic anhydride and activated 4A molecular sieves are added to the turnip flower extract for esterification modification, which can enhance the stability of the active ingredients in the turnip flower extract and improve its ability to inhibit pancreatic lipase.

[0039] Experiment 2: The same mass of the composition with the function of accelerating fat decomposition prepared in Examples 1-3 and Comparative Examples 1-6 were prepared with anhydrous ethanol to prepare a sample solution with a concentration of 10 mg / L. 3T3-L1 adipocytes and the sample solution were incubated at 37°C and 5% CO 2 The cells were incubated in a cell culture incubator for 24 hours, the supernatant was collected and centrifuged at 10,000 rpm for 5 minutes, the glycerol content in the supernatant was measured according to the instructions of the glycerol enzyme assay kit (E1002) and recorded as M1, the sample solution was replaced with anhydrous ethanol and the glycerol content was measured in the above steps and recorded as M2, the glycerol release growth rate was calculated as (M1-M2) / M2×100%, the higher the glycerol release growth rate, the better the sample solution's effect on fat decomposition, the experiments were repeated twice in parallel, the data were recorded and tabulated, as shown in Table 2.

[0040] Table 2: Growth rate of glycerol release from adipocytes after treatment with the composition

[0041] It can be seen from the data of Example 1 and Comparative Examples 1-4 in Table 2 that compounding the noni fruit-pear cactus composite fermentation powder, silkworm pupa polypeptide-turnip flower extract compound, wax apple extract, laver extract and inulin can accelerate the decomposition of body fat and effectively treat obesity.

[0042] It can be seen from the data of Example 1 and Comparative Examples 5-6 in Table 2 that the silkworm pupa polypeptide-turnip flower extract composite prepared by extracting dry powder of silkworm pupa polypeptide and turnip flower can synergistically enhance the ability to decompose subcutaneous fat and visceral fat, and adding succinic anhydride and activated 4A molecular sieve to the turnip flower extract for esterification modification can also improve its ability to decompose fat.

[0043] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A method for preparing a composition having the function of accelerating fat decomposition, characterized in that: The following steps are involved: S1: Preparation of silkworm pupa polypeptides The silkworm pupae are homogenized, freeze-dried, crushed and ethanol-extracted, and the precipitate is collected and dried, and then water is added to the homogenate for enzymatic hydrolysis, and the enzyme is inactivated and then centrifuged to obtain the supernatant, which is freeze-dried to obtain the silkworm pupae polypeptide; S2: Preparation of silkworm pupa polypeptide-turnip flower extract compound The whole turnip flower is washed and crushed, and then added to a solution mixed with choline chloride, glycerol and DL-tartaric acid at high temperature, and then ultrasonically extracted and centrifuged to obtain the supernatant, and succinic anhydride and activated 4A molecular sieve are added to react, and then dried and mixed with silkworm pupa polypeptide and water, and freeze-dried and crushed to obtain a silkworm pupa polypeptide-turnip flower extract composite; S3: Preparation of Noni-Prickly Pear Cactus Composite Fermentation Powder and Compounding of Extracts Add water to the noni fruit and boil it, then add the prickly pear cactus extract, white sugar and pectinase, inoculate Pichia kluyveri for fermentation, centrifuge to collect the supernatant, freeze-dry, and grind to obtain the noni fruit-prickly pear cactus composite fermentation powder, mix it evenly with the silkworm pupa polypeptide-turnip flower extract compound, inulin and stachyose, dry and grind it, then add the wax apple extract, laver extract and resistant dextrin and mix evenly to obtain a composition with the function of accelerating fat decomposition.

2. The method for preparing a composition having the function of accelerating fat decomposition according to claim 1, characterized in that: Step S1: Preparation of silkworm pupa polypeptide, comprising the following steps: S1.1: Take 4-5 portions of silkworm pupae and wash them clean, then add water to the mixture at a solid-liquid ratio of 1:(2-3) g / mL to make a homogenate for freeze-drying, crush and pass through a 30-80 mesh sieve to obtain silkworm pupa powder, mix the silkworm pupa powder with a 90-95% ethanol solution at a solid-liquid ratio of 1:(12-15) g / mL for extraction, the extraction temperature is 30-35°C, the extraction time is 8-10 hours, then centrifuge and filter, collect the precipitate and dry it, and obtain defatted silkworm pupa powder; S1.2: Add water to the defatted silkworm pupa powder at a solid-liquid ratio of 1: (3-4) g / mL to homogenize, adjust the pH to 4-5, first add 2-3% of the weight of cellulase to the defatted silkworm pupa powder, hydrolyze at 50-55°C for 4-6 hours, inactivate the enzyme in a boiling water bath for 10-12 minutes, then cool to 50-55°C, adjust the pH to 8.5-9, then add 3-4% of the weight of protease to the defatted silkworm pupa powder, hydrolyze for 6-8 hours to obtain an enzymolysis solution, inactivate the enzyme in a boiling water bath for 10-12 minutes, quickly cool to room temperature, centrifuge at 8000-10000 rpm at 3-4°C for 12-15 minutes, collect the supernatant and freeze-dry to obtain silkworm pupa polypeptides.

3. The method for preparing a composition having the function of accelerating fat decomposition according to claim 2, characterized in that: Step S2: Preparation of the silkworm pupa polypeptide-turnip flower extract compound, comprising the following steps: S2.1: Wash and dry the whole turnip flower, and then crush it to obtain turnip flower powder, mix choline chloride, glycerol and DL-tartaric acid in a molar ratio of 1: (2-3): (0.5-0.6) in a container, stir continuously at 100-105° C. for 25-30 minutes, cool to room temperature, add 4-6wt% turnip flower powder, and then transfer to an ultrasonic extractor for ultrasonic extraction, and then centrifuge at a speed of 4000-5000rpm for 6-8 minutes in a centrifuge, take the supernatant and filter through a filter membrane with a pore size of 0.2-0.5um to obtain a turnip flower extract; S2.2: Add 1-2wt% of succinic anhydride and 1.5-3wt% of activated 4A molecular sieve to the turnip flower extract, mix them evenly with an ultrasonic extractor at a power of 100-150W, heat them in a water bath to carry out esterification modification reaction, filter them after the reaction to remove succinic anhydride and activated 4A molecular sieve, and vacuum dry them to obtain dry powder; S2.3: Place the dry powder and silkworm pupa polypeptide in a container at a mass ratio of 1: (2-3), add 80-100 times the mass of ultrapure water, then stir at 25-30°C at a stirring speed of 800-1000rpm for 2-2.5 hours, freeze-dry at -50°C--40°C, and then crush to obtain a silkworm pupa polypeptide-turnip flower extract complex.

4. The method for preparing a composition having the function of accelerating fat decomposition according to claim 3, characterized in that: Step S3: Preparation of Noni Fruit-Opuntia Cacti Composite Fermentation Powder and Compounding and Mixing of Extracts, including the following steps: S3.1: Take 2-3 parts of prickly pear cactus, remove the hair and thorns on the surface, then rinse, cut and crush, add 3-5 times the weight of water and boil for 2-3 hours, filter to obtain an extract, repeat 1-2 times to combine the extracts to obtain a prickly pear cactus extract, take 3-4 parts of fresh and ripe noni fruit, clean and crush, add 3-5 times the weight of water and boil for 1.5-2 hours to obtain noni pulp, mix the prickly pear cactus extract and noni pulp, add 10-12wt% of white sugar and 1-2wt% of pectinase, and then inoculate Pichia kluyveri at an inoculum amount of 3-5% for fermentation to obtain a fermentation material; S3.2: Filter the fermented material with a mesh screen to obtain fermentation filtrate I, centrifuge the fermentation filtrate I in a centrifuge at a speed of 5000-6000 rpm for 15-20 minutes, filter the supernatant through a filter membrane to obtain fermentation filtrate II, freeze-dry the fermentation filtrate II at minus 70°C to minus 50°C, and crush to obtain noni fruit-pear cactus composite fermentation powder; S3.3: Mix 10-15 parts of noni fruit-prickly pear cactus composite fermentation powder, 8-10 parts of silkworm pupa polypeptide-turnip flower extract compound, 2-3 parts of inulin and 0.5-1 part of stachyose, dry and crush, add 3-5 parts of wax apple extract, 2-4 parts of laver extract and 10-15 parts of resistant dextrin and mix evenly to obtain a composition with the function of accelerating fat decomposition.

5. The method for preparing a composition having the function of accelerating fat decomposition according to claim 2, characterized in that: The protease in step S1.2 is alkaline protease.

6. The method for preparing a composition having the function of accelerating fat decomposition according to claim 3, characterized in that: In step S2.1, the temperature of ultrasonic extraction is 45-50°C, the ultrasonic power is 150-200W, the ultrasonic frequency is 25-30kHz, and the extraction time is 30-50 minutes.

7. The method for preparing a composition having the function of accelerating fat decomposition according to claim 3, characterized in that: The water bath heating temperature in step S2.2 is 60-65° C., and the water bath time is 4-5 hours.

8. The method for preparing a composition having the function of accelerating fat decomposition according to claim 4, characterized in that: The fermentation temperature in step S3.1 is 25-30° C. and the fermentation time is 8-10 days.

9. The method for preparing a composition having the function of accelerating fat decomposition according to claim 4, characterized in that: The pore size of the filter membrane in step S3.2 is 0.8-1 um.

10. A composition having the function of accelerating fat decomposition, characterized in that: The composition is prepared by the method for preparing a composition having the function of accelerating fat decomposition as described in any one of claims 1 to 9.

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