Anti-wrinkle, firming and skin-moistening cosmetic and preparation method thereof

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to an anti-wrinkle, firming and skin-moistening cosmetic and a preparation method thereof. The anti-wrinkle, firming and skin-moistening cosmetic is prepared from the following raw materials in percentage by weight: 5.5%-6.5% of composite polypeptide freeze-dried powder, 2%-3% of a plant extract, 7%-9% of a humectant, 2.5%-3.5% of an emulsifier, 10%-15% of an emollient, 0.1%-0.3% of a preservative and the balance of water. According to the preparation method, specific polypeptide compounding and freeze-drying treatment are selected, meanwhile, effective components in plants are scientifically extracted, and the molecular weight graded hyaluronic acid compound humectant and the nano-liposome emollient are combined, so that the prepared cosmetic not only can achieve the effects of resisting wrinkles, tightening skin and moistening skin at the same time, but also is good in use feeling and high in stability.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to an anti-wrinkle, firming and moisturizing cosmetic and a preparation method thereof. Background Art

[0002] As people's living standards improve, the demand for skin care is growing. As the largest organ in the human body, the skin will inevitably age with age. In addition, environmental factors such as light, radiation, pollution, high work pressure, irregular work and rest schedules can also accelerate skin aging. Skin aging is mainly manifested in abnormal stratum corneum, increased wrinkles, reduced skin elasticity, dryness and dehydration, and thinning of the skin.

[0003] There are many kinds of cosmetics on the market. For example, facial creams are rich in variety, mainly including moisturizing creams, whitening creams, anti-aging creams, etc. Moisturizing creams can maintain the moisture of the skin, whitening creams can improve the skin tone, and anti-aging creams can reduce the appearance of fine lines and wrinkles. However, the effects of various facial creams are relatively single, such as acting on moisturizing alone, or only targeting anti-wrinkle repair, and some are only whitening and spot-lightening, which cannot meet consumers' needs for comprehensive skin care.

[0004] At the same time, existing anti-wrinkle and firming creams have some problems. In the prior art, although some products have added polypeptide ingredients to promote collagen production and improve wrinkles to a certain extent, the effect of a single polypeptide is limited, and the activity of polypeptides is easily affected in complex formula systems and storage processes, resulting in reduced efficacy. At the same time, some products that claim to have a firming effect often rely on certain chemical synthetic ingredients. Although they can make the skin feel firm in the short term, long-term use may cause irritation to the skin and pose safety risks.

[0005] In terms of skin moisturizing, many cosmetics use traditional oils and moisturizers, such as glycerin and sodium hyaluronate, which can replenish and lock skin moisture to a certain extent, but are not ideal for deep skin moisturizing and improving skin barrier function. In addition, the formulas of some products are not scientific enough, and the synergy between different raw materials is poor, making it difficult to achieve long-term skin moisturizing. In addition, some moisturizing products have a greasy texture, which affects the feeling of use and is especially not suitable for people with oily skin.

[0006] Therefore, it is of great practical significance to develop a cosmetic that can simultaneously achieve anti-wrinkle, firming and moisturizing effects, and has a good feel and high stability. It is urgent to provide new anti-wrinkle, firming and moisturizing cosmetics and their preparation raw materials to solve the above technical problems. Summary of the invention

[0007] The purpose of the present invention is to provide an anti-wrinkle, firming and moisturizing cosmetic and a preparation method thereof, which can not only achieve the anti-wrinkle, firming and moisturizing effects at the same time, but also has good use experience and high stability.

[0008] An anti-wrinkle, firming and moisturizing cosmetic, the preparation raw materials of which, by weight percentage, include 5.5%-6.5% of compound polypeptide freeze-dried powder, 2%-3% of plant extract, 7%-9% of moisturizer, 2.5%-3.5% of emulsifier, 10%-15% of moisturizer, 0.1%-0.3% of preservative, and the balance is supplemented with water to 100%.

[0009] The preparation method of the composite polypeptide freeze-dried powder comprises the following steps: mixing the active polypeptide, the polypeptide carrier and the protective agent, stirring until completely dissolved, homogenizing, and freeze-drying to obtain the composite polypeptide freeze-dried powder.

[0010] Preferably, the active polypeptide is acetyl hexapeptide-8, palmitoyl tripeptide-1, snake venom peptide, acetyl octapeptide-3.

[0011] Preferably, the mass ratio of acetyl hexapeptide-8, palmitoyl tripeptide-1, snake venom peptide, and acetyl octapeptide-3 is (2-4):(2-3):(1-2):1; more preferably, it is 6:5:3:2.

[0012] By selecting specific active peptides as raw materials for anti-wrinkle, firming and moisturizing cosmetics, the anti-wrinkle effect of cosmetics can be improved while improving their moisturizing effect. This may be because several peptides work synergistically to repair the dermal matrix while anti-wrinkle at multiple targets. Acetyl hexapeptide-8 and acetyl octapeptide-3 work synergistically to inhibit neurotransmitter release, reduce muscle contraction, and block nerve signals. Snake venom peptides, palmitoyl tripeptide-1, and acetyl octapeptide-3 work synergistically to first inhibit muscle contraction by simulating snake venom toxins, quickly reduce expression lines in the short term, and then continue to stimulate collagen regeneration while inhibiting muscle contraction, and improve the anti-wrinkle effect through fast-acting and long-term linkage. Acetyl octapeptide-3 and palmitoyl tripeptide-1 compound can also repair the dermal matrix while reducing neuroinflammation, thereby reducing transepidermal water loss and improving moisturizing effect. However, these peptides have poor stability and are easily affected by factors such as temperature, pH, and microorganisms in a liquid environment and lose their activity, thereby affecting the efficacy of cosmetics.

[0013] Preferably, the polypeptide carrier is a β-glucan aqueous solution and hyaluronic acid nanospheres.

[0014] Preferably, the mass ratio of the β-glucan aqueous solution to the hyaluronic acid nanospheres is (4-6):1; further preferably, it is 5:1.

[0015] Preferably, the mass fraction of β-glucan in the β-glucan aqueous solution is 3%.

[0016] Preferably, the particle size of the hyaluronic acid nanospheres is 50-100 nm.

[0017] In some preferred embodiments, the hyaluronic acid nanospheres are from Nanjing Chico Biotechnology Co., Ltd.

[0018] Preferably, the protective agent includes trehalose and mannitol.

[0019] Preferably, the mass ratio of trehalose to mannitol is 1:(0.5-2); further preferably, it is 1:1.

[0020] Preferably, the mass ratio of the active polypeptide, the polypeptide carrier and the protective agent is 1:(1.5-2.5):(0.8-1.2).

[0021] The specific steps of the homogenization are: adjusting the temperature to 4°C, homogenizing once at a pressure of 500 bar for 3 minutes, and then homogenizing twice at 800 bar for 2 minutes each time.

[0022] The specific steps of freeze-drying are: cooling to -50°C at a rate of 1°C / min, maintaining for 6 hours, adjusting the vacuum degree to 0.1 Pa, heating to -20°C at 0.3°C / min, maintaining for 48 hours, heating to 25°C at 0.3°C / min, and maintaining for 36 hours.

[0023] By selecting a specific freeze-dried powder preparation method, active peptides are prepared into composite peptide freeze-dried powder, which can maximize the retention of the biological activity and stability of the peptides, thereby improving the efficacy of cosmetics. This may be because firstly a specific peptide carrier is selected to improve the permeability and stability of the peptides, reduce irritation to the skin, and the protective agent forms a glassy protective layer to prevent the peptides from denaturing during the freeze-drying process. Finally, gradient pressure homogenization and gradient freeze-drying are used to ensure that the peptides are evenly dispersed and the biological activity of the peptides is retained to the maximum extent.

[0024] Preferably, the plant extract is Eryngium littoralis ( SEA LYME ) extract, Centella asiatica extract and Panax ginseng extract.

[0025] Preferably, the mass ratio of the Eryngium marineum extract, Centella asiatica extract and Panax ginseng extract is 1:(1.3-1.8):(1.5-2.5); further preferably, it is 2:3:4.

[0026] By selecting Eryngium marinum extract, Centella asiatica extract and Ginseng extract as plant extracts, the effective components of each ingredient are utilized, which can not only promote the regeneration of collagen and cells, but also improve the antioxidant and anti-inflammatory effects of cosmetics, thereby improving the protective effect and barrier repair function of the skin. This may be because the effective ingredients in the three plants are extracted by a specific method, and these effective ingredients have a synergistic effect. Eryngium marinum is rich in polyphenols and flavonoids. Its strong antioxidant ability can neutralize the damage of free radicals to collagen fibers, providing the first line of defense for anti-wrinkle; the triterpenoids in Centella asiatica (such as madecassic acid) reconstruct the supporting structure of the dermis by promoting fibroblast proliferation and collagen synthesis, and its anti-inflammatory properties help alleviate skin aging caused by chronic micro-inflammation; ginsenosides in ginseng extract not only activate the AMPK pathway to enhance cell metabolic activity, but also optimize skin hydration by upregulating hyaluronic acid synthase gene expression. The three ingredients form a closed loop of "antioxidant defense-collagen remodeling-hydration regulation" in terms of mechanism of action: Eryngium coastalis removes free radicals and reduces collagen degradation, Centella asiatica accelerates collagen regeneration and remodels the skin skeleton, and ginseng strengthens cell metabolism and locks in moisture. The three ingredients work together to achieve the triple effects of wrinkle reduction, firmness improvement, and long-lasting moisturizing. The various effects of cosmetics are significantly enhanced through the combined action of multiple dimensions and multiple targets. The preparation method of the Eryngium coastalis extract comprises the following steps: A1. Take the stems and leaves of Eryngium littoralis, dry them separately, and then crush them (for example, crush them to a particle size of less than 100 μm), so as to obtain pretreated Eryngium littoralis; A2, activating the fermentation agent to obtain an activation solution; after uniformly mixing the pretreated Eryngium marinum and glucose, adding water to adjust the water content (for example, the water content is 45wt%-55wt%) to obtain a fermentation substrate, inoculating the activation solution and fermenting at 25-40°C for 40-100h to obtain a fermentation product; A3. Mix the fermentation product with deionized water, perform ultrasonic water extraction and centrifugation, and filter the supernatant through an ultrafiltration membrane to obtain a retained macromolecular protein with a molecular weight of >10 kDa. After dialyzing with pure water, the supernatant is spray-dried to obtain the product.

[0027] Preferably, the mass ratio of the stems to the leaves is 1:2.

[0028] Preferably, the added amount of glucose is 2%-4% of the mass of the pretreated Eryngium littoralis.

[0029] Preferably, the fermentation agent is Lactobacillus plantarum.

[0030] The plant lactobacillus strain is numbered CICC 24936.

[0031] The preparation method of the activation solution comprises the following steps: inoculating Lactobacillus plantarum in an MRS culture medium, culturing in an anaerobic tank at 37°C for 24 hours, and ensuring that the number of viable bacteria collected by centrifugation is ≥1×1010 CFU / mL, and the activation solution is obtained.

[0032] Preferably, the amount of Lactobacillus plantarum is 10 8 -10 9 CFU / g fermentation substrate.

[0033] Preferably, the mass ratio of the fermentation product to deionized water is 1:(7-9).

[0034] The specific conditions of the ultrasonic water extraction are: frequency of 40kHz, power of 500W, temperature of 45°C, time of 30min, mode of pulse mode, and ultrasound is turned off for 2s after every 5s of operation.

[0035] The preparation method of the Centella asiatica extract comprises the following steps: B1. Wash the leaves of Centella asiatica, dry them, and crush them to obtain Centella asiatica powder. Add water to adjust the water content to 40%-50%, adjust the temperature to 45-50° C., and adjust the pH to 4.5-5.5. Add cellulase, perform enzymolysis for 2-3 hours, and then boil and inactivate to obtain Centella asiatica hydrolysate. B2. Add 80°C hot water to the Centella asiatica hydrolysate for extraction twice, each time for 1-2 hours. Combine the filtrates, concentrate under reduced pressure, and dry to a moisture content of ≤0.1% to obtain the Centella asiatica extract.

[0036] Preferably, the added amount of the cellulase is 80-120 U / g Centella asiatica powder.

[0037] Preferably, the mass ratio of the Centella asiatica hydrolysate to 80°C hot water is 1:(10-20).

[0038] The preparation method of the ginseng extract comprises the following steps: C1. Wash the ginseng root, dry it, and crush it to obtain ginseng root powder. Reflux extract it with 70% ethanol aqueous solution for 3 times, combine the extracts, and concentrate it to 25% of the volume of the extract to obtain a concentrated solution. Add 3 times the volume of 95% ethanol aqueous solution, let it stand at 4°C for more than 12 hours, and centrifuge to obtain a precipitate and a supernatant; C2, extracting the supernatant with supercritical carbon dioxide and then rotary evaporating to obtain a solid; C3. Mix the precipitate and the solid to obtain the ginseng extract.

[0039] Preferably, the mass ratio of the ginseng root powder to the 70% by mass ethanol aqueous solution is 1:(10-20).

[0040] Preferably, the specific conditions of the supercritical carbon dioxide extraction are: pressure 23-28 MPa, carbon dioxide flow rate 18-22 L / h, entrainer flow rate 0.5-1.5 mL / min, extraction time 3.5-4.5 h, and extraction temperature 43-48°C.

[0041] Preferably, the entrainer is an ethanol aqueous solution with a mass fraction of 65%-75%.

[0042] By targeting the active ingredients of these three plants and adopting specific lactic acid bacteria fermentation, enzymatic hydrolysis and supercritical carbon dioxide extraction processes, the bound flavonoids in Eryngium coastalis are converted into free states, thereby improving the bioavailability; enzymatic hydrolysis destroys the cell walls and increases the yield of triterpenoid compounds; supercritical carbon dioxide extraction selectively enriches small molecule saponins such as Rg3, greatly increasing the content of active ingredients in each extract, thereby improving its efficacy in cosmetics.

[0043] Preferably, the moisturizing agent is hyaluronic acid complex, sodium polyglutamate and glycerin.

[0044] Preferably, the mass ratio of the hyaluronic acid complex, sodium polyglutamate and glycerol is (1-2):1:(1-2); further preferably, it is 3:2:3.

[0045] The preparation method of the hyaluronic acid composite comprises the following steps: respectively adding deionized water to macromolecular hyaluronic acid, medium molecular hyaluronic acid, and small molecular hyaluronic acid to prepare macromolecular hyaluronic acid gel, medium molecular hyaluronic acid gel, and small molecular hyaluronic acid gel, then pouring the medium molecular hyaluronic acid gel into the small molecular hyaluronic acid gel for homogenization (for example, homogenization at 800 bar for 3 minutes), pouring the macromolecular hyaluronic acid gel into the gel, homogenizing (for example, homogenizing at 500 bar for 5 minutes) to obtain a composite gel, and freeze-drying the composite gel to remove the deionized water. The temperature is controlled below 20° C. during homogenization.

[0046] Preferably, the molecular weight of the macromolecular hyaluronic acid is 1500k-2500kDa; the molecular weight of the medium-molecular hyaluronic acid is 40k-100kDa; and the molecular weight of the small-molecular hyaluronic acid is 1k-10kDa.

[0047] In some preferred embodiments, the macromolecular hyaluronic acid and medium-molecular hyaluronic acid are both from Shanghai MacLean Biochemical Technology Co., Ltd.; the small-molecule hyaluronic acid is from Blue Power Biotechnology (Xi'an) Co., Ltd.

[0048] Preferably, the mass ratio of the macromolecular hyaluronic acid, the medium molecular hyaluronic acid and the small molecular hyaluronic acid is (1-1.5):(1.3-1.8):1; further preferably, it is 5:6:4.

[0049] By selecting hyaluronic acid with different molecular weights, the moisturizing property of cosmetics can be improved. This may be because large molecular hyaluronic acid forms a water-locking film to reduce transepidermal water loss, medium molecular hyaluronic acid penetrates into the dermis and promotes basal cell proliferation, and small molecular hyaluronic acid activates aquaporin to enhance the cell's water absorption capacity. The three molecular weights of hyaluronic acid work synergistically to achieve deep moisturizing through the triple effects of large molecule water lock, medium molecule penetration, and small molecule activation, greatly increasing the water content of the stratum corneum. Moreover, the medium molecular hyaluronic acid that penetrates into the dermis can also stimulate fibroblast proliferation and promote collagen production in synergy with polypeptides. However, directly mixing the three molecular weights of hyaluronic acid will cause the large molecular hyaluronic acid to form a viscous layer on the surface of the solution, wrapping the medium and small molecular hyaluronic acid, hindering its deep penetration, thereby affecting the moisturizing effect. In addition, the charge density and intermolecular forces of hyaluronic acid with different molecular weights are very different. Direct mixing may cause cross-linking or coagulation, affecting the stability of skin care products.

[0050] Preferably, the mass fractions of the macromolecular hyaluronic acid, medium molecular hyaluronic acid and small molecular hyaluronic acid in the macromolecular hyaluronic acid gel, medium molecular hyaluronic acid gel and small molecular hyaluronic acid gel are all 3%-5%.

[0051] The preparation method of the macromolecular hyaluronic acid gel comprises the following steps: adding deionized water at 80° C. to the macromolecular hyaluronic acid, standing at room temperature for 30-50 minutes, and stirring at 300 rpm for 2 hours to obtain the macromolecular hyaluronic acid gel.

[0052] The preparation method of the medium molecular hyaluronic acid gel comprises the following steps: adding 50° C. deionized water to the medium molecular hyaluronic acid, and stirring at 50° C. and 300 rpm for 1 hour to obtain the gel.

[0053] The preparation method of the small molecule hyaluronic acid gel comprises the following steps: adding deionized water at 25° C. to the small molecule hyaluronic acid, and stirring at a rotation speed of 2500 rpm for 5-10 minutes to obtain the gel.

[0054] The specific steps of freeze-drying are: cooling to -50°C at a rate of 1°C / min, maintaining for 6 hours, adjusting the vacuum degree to 0.1 Pa, heating to -20°C at 0.3°C / min, maintaining for 48 hours, heating to 25°C at 0.3°C / min, and maintaining until the mass no longer decreases.

[0055] By preparing hyaluronic acid of different molecular weights into hyaluronic acid gel respectively and mixing them in a gradient from small to large, small molecules are prevented from being wrapped by large molecules, enhancing the stability of the system while forming a bionic layered structure, reducing the transepidermal water loss rate. At the same time, synergistically with the other two moisturizers, the negative charge of sodium polyglutamate forms an electrostatic grid with hyaluronic acid, cross-linking into a film, reducing water evaporation while playing a role in barrier repair, greatly increasing the water content of the stratum corneum while prolonging the moisturizing time. Glycerin can not only achieve instant moisturizing effect, but also reduce the stickiness of hyaluronic acid and improve comfort in use.

[0056] Preferably, the molecular weight of the sodium polyglutamate is ≤100 kDa.

[0057] In some preferred embodiments, the sodium polyglutamate comes from Shanghai MacLean Biochemical Technology Co., Ltd.

[0058] Preferably, the emulsifier comprises PEG-7 olivetate.

[0059] Preferably, the HLB value of the PEG-7 olive oil ester is 11, and the hydroxyl value is 170-185 mg KOH / g.

[0060] In some preferred embodiments, the PEG-7 olive oil ester comes from Wuhan Huaxiang Kejie Biotechnology Co., Ltd.

[0061] Preferably, the emollient comprises caprylic / capric triglyceride, meadowfoam seed oil, and camellia oil nanoliposomes in a mass ratio of (1.5-2):(1-1.5):1; more preferably, it is 5:4:3.

[0062] Selecting caprylic / capric triglyceride, white meadowfoam seed oil, and camellia oil as emollients can not only improve the anti-wrinkle and firming properties of cosmetics, but also improve their moisturizing properties and reduce transepidermal water loss. This may be due to the synergistic effect of the three. On the one hand, white meadowfoam seed oil provides ceramide analogs such as white meadowfoam oligolactide, and camellia oil supplements ω-3 / ω-6 fatty acids. The two supplement lipid components; caprylic / capric triglyceride acts as a "liquid scaffold" to fill the gaps in the stratum corneum lamellar structure; at the same time, squalene in camellia oil activates β-glucocerebrosidase and accelerates the synthesis of barrier lipids. The three work synergistically to complete the barrier repair matrix, thereby improving the moisturizing effect and reducing the transepidermal water loss rate. On the other hand, white meadowfoam seed oil inhibits the activity of matrix metalloproteinase-1, reduces collagen degradation, and protects collagen in the skin; the linolenic acid in camellia oil is converted into prostaglandin E2, upregulating the expression of transforming growth factor and promoting the generation of new cells; caprylic / capric triglyceride forms an elastic film on the skin surface, providing an immediate sense of firmness. The combination of the three forms a dual anti-wrinkle and firming pathway, reducing the area and depth of fine lines on the surface. However, camellia oil is easily oxidized, resulting in poor system stability, and to achieve better moisturizing and anti-wrinkle effects, the oleic acid in camellia oil needs to penetrate the stratum corneum to stimulate fibroblasts.

[0063] The preparation method of camellia oil nanoliposomes comprises the following steps: mixing camellia oil, hydrogenated lecithin, cholesterol, co-solvent and stabilizer, ultrasonically emulsifying for 5 minutes, placing in a high-pressure reactor for supercritical carbon dioxide treatment, and homogenizing after decompression (for example, homogenizing for 5 minutes at a pressure of 80 bar and a temperature of 15° C.).

[0064] Preferably, the co-solvent is an ethanol aqueous solution with a mass fraction of 95%.

[0065] Preferably, the stabilizer is a mixed aqueous solution of poloxamer 188 and trehalose, wherein the mass fraction of poloxamer 188 is 3% and the mass fraction of trehalose is 5%.

[0066] Preferably, the mass ratio of camellia oil, hydrogenated lecithin and cholesterol is (25-35):(4-6):1; more preferably, it is 30:5:1.

[0067] Preferably, the added amount of the co-solvent is 10%-15% of the total mass of camellia oil, hydrogenated lecithin and cholesterol.

[0068] Preferably, the added amount of the stabilizer is 2.5-3 times the total mass of camellia oil, hydrogenated lecithin and cholesterol.

[0069] Preferably, the specific conditions of the ultrasonic emulsification are: frequency of 40 kHz, power of 150 W, and temperature of 25°C.

[0070] Preferably, the conditions for the supercritical carbon dioxide treatment are: pressure of 20 MPa, temperature of 45° C., and time of 30 min.

[0071] By using hydrogenated phospholipin and cholesterol to jointly construct the camellia oil nanoliposome skeleton, not only can its stability be improved and its active ingredients in the system be protected, but also its utilization efficiency in skin care is significantly improved. This may be due to the synergistic effect between hydrogenated phospholipin and cholesterol. As the core builder of liposomes, hydrogenated phospholipin spontaneously forms nano-scale vesicles during film dispersion or emulsification through its unique amphiphilic structure. These vesicles not only tightly wrap the active ingredients such as polyphenols and squalene in camellia oil to prevent them from being oxidized or degraded during storage or application, but also rely on its strong skin affinity to efficiently fuse with the skin cell membrane, significantly promoting the penetration of camellia oil into the basal layer of the skin. At the same time, cholesterol, as a key regulator of liposome stability, is precisely embedded in the phospholipid bilayer. By limiting the excessive fluidity of the membrane, it builds a solid barrier for the liposome vesicles at the macro level, effectively resisting the damage of external factors such as temperature changes and mechanical stress, ensuring the integrity of the liposomes during the storage period and the long-term retention of active ingredients; at the micro level, cholesterol and hydrogenated lecithin synergistically regulate the particle size distribution of liposomes, making the vesicle size more uniform, thereby optimizing the spreadability and absorption efficiency of camellia oil on the skin surface. Through dynamic functional complementarity, the two not only achieve multiple protections for the active ingredients of camellia oil, but also precisely regulate its release and absorption process in the time and space dimensions, ultimately significantly improving the utilization efficiency of camellia oil in skin care. In addition, the supercritical carbon dioxide method is used instead of the traditional chloroform method to prepare camellia oil nanoliposomes, which not only has no solvent residue, is safe and non-toxic, and is suitable for the cosmetics field, but also has a stable encapsulation rate and a narrow particle size distribution. However, camellia oil has low solubility in the supercritical carbon dioxide system, so certain co-solvents and stabilizers need to be added to increase the lipid solubility while improving the stability, thereby increasing the encapsulation rate and further improving the moisturizing effect of emollients and cosmetics.

[0072] In some preferred embodiments, the meadowfoam seed oil and camellia oil are both from Jiangxi Zhonghuan Biotechnology Co., Ltd.

[0073] Preferably, the preservative comprises 1,2-hexanediol and p-hydroxyacetophenone in a mass ratio of 5:3.

[0074] The method for preparing the anti-wrinkle, firming and moisturizing cosmetic comprises the following steps: S1. Preparation of aqueous phase: mixing the moisturizer and water, and stirring until completely dissolved to obtain an aqueous phase; S2. Preparation of oil phase: After mixing the emollients, add the emulsifier and stir evenly (for example, stir at 1000 rpm for 10 min until evenly mixed) to obtain the oil phase; S3. Homogenization and emulsification: heat the water phase and the oil phase separately (for example, heat to 45°C), pour the oil phase into the water phase, homogenize in a homogenizer (for example, at a pressure of 800 bar for 5 minutes), cool down (for example, cool to 30°C), add the composite polypeptide freeze-dried powder and the plant extract, stir evenly, add the preservative, continue to stir evenly, and degas in vacuum to obtain.

[0075] Compared with the prior art, the advantages and beneficial effects of the present invention are: 1. The anti-wrinkle, firming and moisturizing cosmetics prepared by the present invention are prepared by selecting specific polypeptide compounding and freeze-drying treatment, and scientifically extracting effective ingredients from plants, and combining molecular weight graded hyaluronic acid complex moisturizers and nanoliposome moisturizers, so that the prepared cosmetics can not only achieve anti-wrinkle, firming and moisturizing effects at the same time, but also have good use experience and high stability.

[0076] 2. The present invention selects specific active polypeptides as raw materials for anti-wrinkle, firming and moisturizing cosmetics, thereby improving the anti-wrinkle effect of cosmetics while improving their moisturizing effect; at the same time, a specific freeze-dried powder preparation method is adopted to prepare the active polypeptides into composite polypeptide freeze-dried powder, which can retain the biological activity and stability of the polypeptides to the maximum extent, thereby improving the efficacy of the cosmetics.

[0077] 3. The present invention selects Eryngium maritima extract, Centella asiatica extract and ginseng extract as plant extracts, and utilizes the effective components of each component, which can not only promote the regeneration of collagen and cells, but also improve the antioxidant and anti-inflammatory effects of cosmetics, thereby improving the protective effect and barrier repair function of the skin.

[0078] 4. The present invention selects hyaluronic acid with different molecular weights for compounding, and prepares hyaluronic acid gel with different molecular weights respectively, and mixes them in a gradient from small to large, which can improve the moisturizing property of cosmetics while improving the stability of the system.

[0079] 5. The present invention selects caprylic / capric triglyceride, meadowfoam seed oil, and camellia oil as emollients, which can not only improve the anti-wrinkle and firming properties of cosmetics, but also improve their moisturizing properties and reduce transepidermal water loss rate.

[0080] 6. The present invention selects hydrogenated phospholipids and cholesterol to jointly construct the camellia oil nanoliposome skeleton, which not only improves its stability and protects its active ingredients in the system, but also significantly improves its utilization efficiency in skin care. DETAILED DESCRIPTION

[0081] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0082] The following raw materials used in the present invention are commercially available, specifically: Hyaluronic acid nanospheres, with a particle size of 50-100 nm, were obtained from Nanjing Jike Biotechnology Co., Ltd.

[0083] Lactobacillus plantarum, strain number CICC 24936, was obtained from the China Industrial Microbiological Culture Collection Center.

[0084] MRS medium was from Guangdong Huankai Microbiological Technology Co., Ltd.

[0085] Cellulase, with an activity of about 11,000 U / g, was obtained from Ningxia Xiasheng Industrial Group Co., Ltd.

[0086] Large molecular weight hyaluronic acid, molecular weight is 1500k-2500kDa; medium molecular weight hyaluronic acid, molecular weight is 40k-100kDa; both are from Shanghai McLean Biochemical Technology Co., Ltd.

[0087] Small molecule hyaluronic acid, with a molecular weight of 1k-10kDa, comes from Blue Power Biotechnology (Xi'an) Co., Ltd.

[0088] Sodium polyglutamate, molecular weight ≤100 kDa, was obtained from Shanghai MacLean Biochemical Technology Co., Ltd.

[0089] PEG-7 olive oil ester, HLB value is 11, hydroxyl value is 170-185 mg KOH / g, from Wuhan Huaxiang Kejie Biotechnology Co., Ltd.

[0090] Meadowfoam seed oil and camellia oil, both from Jiangxi Zhonghuan Biotechnology Co., Ltd.

[0091] Example 1 The present embodiment provides an anti-wrinkle, firming and moisturizing cosmetic, the raw materials for its preparation, by weight percentage, are 6.0% of composite polypeptide freeze-dried powder, 2.5% of plant extract, 8% of moisturizer, 3% of emulsifier, 12.5% ​​of moisturizer, 0.2% of preservative, and the balance is supplemented with water to 100%.

[0092] The preparation method of the composite polypeptide freeze-dried powder comprises the following steps: mixing the active polypeptide, the polypeptide carrier and the protective agent, stirring until completely dissolved, homogenizing, and freeze-drying to obtain the composite polypeptide freeze-dried powder.

[0093] The active polypeptides are acetyl hexapeptide-8, palmitoyl tripeptide-1, snake venom peptide, and acetyl octapeptide-3, with a mass ratio of 6:5:3:2.

[0094] The polypeptide carrier is a β-glucan aqueous solution and hyaluronic acid nanospheres, with a mass ratio of 5:1.

[0095] The mass fraction of β-glucan in the β-glucan aqueous solution is 3%.

[0096] The protective agent is trehalose and mannitol, and the mass ratio is 1:1.

[0097] The mass ratio of the active polypeptide, the polypeptide carrier and the protective agent is 1:2:1.

[0098] The specific steps of the homogenization are: adjusting the temperature to 4°C, homogenizing once at a pressure of 500 bar for 3 minutes, and then homogenizing twice at 800 bar for 2 minutes each time.

[0099] The specific steps of freeze-drying are: cooling to -50°C at a rate of 1°C / min, maintaining for 6 hours, adjusting the vacuum degree to 0.1 Pa, heating to -20°C at 0.3°C / min, maintaining for 48 hours, heating to 25°C at 0.3°C / min, and maintaining for 36 hours.

[0100] The plant extracts are Eryngium maritima extract, Centella asiatica extract and Ginseng extract, with a mass ratio of 2:3:4.

[0101] The preparation method of the Eryngium littoralis extract comprises the following steps: A1. The stems and leaves of Eryngium littoralis are dried and crushed to a particle size of less than 100 μm to obtain pretreated Eryngium littoralis; A2, activating the fermentation agent to obtain an activation solution; after uniformly mixing the pretreated Eryngium marinum and glucose, adding water to adjust the water content to 50wt% to obtain a fermentation substrate, inoculating the activation solution and fermenting at 28°C for 55h to obtain a fermentation product; A3. Mix the fermentation product with deionized water, perform ultrasonic water extraction and centrifugation, and filter the supernatant through an ultrafiltration membrane to obtain a retained macromolecular protein with a molecular weight of >10 kDa. After dialyzing with pure water for 36 hours, spray-dry the supernatant to a water content of 0.05%.

[0102] The mass ratio of the stems to the leaves is 1:2.

[0103] The added amount of glucose is 3% of the mass of the pretreated Eryngium littoralis.

[0104] The preparation method of the activation solution comprises the following steps: inoculating plant lactobacillus in an MRS culture medium, culturing in an anaerobic tank at 37° C. for 24 hours, and ensuring that the number of viable bacteria collected by centrifugation is 1×1010 CFU / mL, and the activation solution is obtained.

[0105] The dosage of the plant lactobacillus is 10 9 CFU / g fermentation substrate.

[0106] The mass ratio of the fermentation product to deionized water is 1:8.

[0107] The specific conditions of the ultrasonic water extraction are: frequency of 40kHz, power of 500W, temperature of 45°C, time of 30min, mode of pulse mode, and ultrasound is turned off for 2s after every 5s of operation.

[0108] The preparation method of the Centella asiatica extract comprises the following steps: B1. Wash the leaves of Centella asiatica, dry them, and grind them to obtain Centella asiatica powder. Add water to adjust the water content to 45%, adjust the temperature to 48° C., adjust the pH to 5, add cellulase, and perform enzymolysis for 2.5 hours before boiling and inactivating the enzyme to obtain Centella asiatica hydrolysate. B2. Add 80°C hot water to the Centella asiatica hydrolysate for extraction twice, each time for 1.5 hours. Combine the filtrates, concentrate under reduced pressure, and dry to a moisture content of 0.05% to obtain the Centella asiatica extract.

[0109] The added amount of the cellulase is 100 U / g Centella asiatica powder.

[0110] The mass ratio of the Centella asiatica hydrolysate to 80°C hot water is 1:15.

[0111] The preparation method of the ginseng extract comprises the following steps: C1. Wash the ginseng root, dry it, and crush it to obtain ginseng root powder. Reflux extract it with 70% ethanol aqueous solution for 3 times. Combine the extracts and concentrate them to 25% of the volume of the extract to obtain a concentrate. Add 95% ethanol aqueous solution with a mass concentration of 3 times the volume of the concentrate, let it stand at 4°C for 12 hours, and centrifuge to obtain a precipitate and a supernatant. C2, extracting the supernatant with supercritical carbon dioxide and then rotary evaporating to obtain a solid; C3. Mix the precipitate and the solid to obtain the ginseng extract.

[0112] The mass ratio of the ginseng root powder to the 70% ethanol aqueous solution is 1:15.

[0113] The specific conditions of the supercritical carbon dioxide extraction are: pressure 25 MPa, carbon dioxide flow rate 20 L / h, entrainer flow rate 1 mL / min, extraction time 4 h, and extraction temperature 45°C.

[0114] The entrainer is an ethanol aqueous solution with a mass fraction of 70%.

[0115] The moisturizing agent is a hyaluronic acid complex, sodium polyglutamate and glycerin, with a mass ratio of 3:2:3.

[0116] The preparation method of the hyaluronic acid composite comprises the following steps: adding deionized water to macromolecular hyaluronic acid, medium molecular hyaluronic acid and small molecular hyaluronic acid to prepare macromolecular hyaluronic acid gel, medium molecular hyaluronic acid gel and small molecular hyaluronic acid gel respectively; then pouring the medium molecular hyaluronic acid gel into the small molecular hyaluronic acid gel and homogenizing at 800 bar for 3 minutes; then pouring the macromolecular hyaluronic acid gel into the small molecular hyaluronic acid gel and homogenizing at 500 bar for 5 minutes to obtain the composite gel; controlling the temperature below 20° C. during homogenization; freeze-drying the composite gel to remove the deionized water to obtain the composite gel.

[0117] The mass ratio of the large molecular hyaluronic acid, the medium molecular hyaluronic acid and the small molecular hyaluronic acid is 5:6:4.

[0118] The mass fractions of the macromolecular hyaluronic acid, medium molecular hyaluronic acid and small molecular hyaluronic acid in the macromolecular hyaluronic acid gel, medium molecular hyaluronic acid gel and small molecular hyaluronic acid gel are all 4%.

[0119] The preparation method of the macromolecular hyaluronic acid gel comprises the following steps: adding deionized water at 80° C. to the macromolecular hyaluronic acid, standing at room temperature for 40 minutes, and stirring at 300 rpm for 2 hours to obtain the macromolecular hyaluronic acid gel.

[0120] The preparation method of the medium molecular hyaluronic acid gel comprises the following steps: adding 50° C. deionized water to the medium molecular hyaluronic acid, and stirring at 50° C. and 300 rpm for 1 hour to obtain the gel.

[0121] The preparation method of the small molecule hyaluronic acid gel comprises the following steps: adding deionized water at 25° C. to the small molecule hyaluronic acid, and stirring at a rotation speed of 2500 rpm for 8 minutes.

[0122] The specific steps of freeze-drying are: cooling to -50°C at a rate of 1°C / min, maintaining for 6 hours, adjusting the vacuum degree to 0.1 Pa, heating to -20°C at 0.3°C / min, maintaining for 48 hours, heating to 25°C at 0.3°C / min, and maintaining until the mass no longer decreases.

[0123] The emulsifier is PEG-7 olive oil ester.

[0124] The emollient comprises caprylic / capric triglyceride, meadowfoam seed oil and camellia oil nanoliposomes, with a mass ratio of 5:4:3.

[0125] The preparation method of the camellia oil nanoliposome comprises the following steps: mixing camellia oil, hydrogenated lecithin, cholesterol, a co-solvent and a stabilizer, performing ultrasonic emulsification for 5 minutes, placing the mixture in a high-pressure reactor for supercritical carbon dioxide treatment, and after decompression, homogenizing the mixture at a pressure of 80 bar and a temperature of 15° C. for 5 minutes to obtain the nanoliposome.

[0126] The co-solvent is an ethanol aqueous solution with a mass fraction of 95%.

[0127] The stabilizer is a mixed aqueous solution of poloxamer 188 and trehalose, wherein the mass fraction of poloxamer 188 is 3% and the mass fraction of trehalose is 5%.

[0128] The mass ratio of the camellia oil, hydrogenated lecithin and cholesterol is 30:5:1.

[0129] The added amount of the co-solvent is 12% of the total mass of camellia oil, hydrogenated lecithin and cholesterol.

[0130] The added amount of the stabilizer is 2.8 times the total mass of camellia oil, hydrogenated lecithin and cholesterol.

[0131] The specific conditions of the ultrasonic emulsification are: frequency of 40kHz, power of 150W, and temperature of 25°C.

[0132] The conditions of the supercritical carbon dioxide treatment are: pressure of 20 MPa, temperature of 45° C., and time of 30 min.

[0133] The preservatives are 1,2-hexanediol and p-hydroxyacetophenone in a mass ratio of 5:3.

[0134] The preparation method of the anti-wrinkle, firming and moisturizing cosmetics comprises the following steps: S1. Preparation of aqueous phase: mixing the moisturizer and water, and stirring until completely dissolved to obtain an aqueous phase; S2, oil phase preparation: after mixing the emollients, add the emulsifier, stir at 1000 rpm for 10 min until uniform, to obtain the oil phase; S3. Homogenization and emulsification: heat the water phase and the oil phase to 45°C respectively, then slowly pour the oil phase into the water phase, process it in a homogenizer at a pressure of 800 bar for 5 minutes, cool it to 30°C, add the freeze-dried powder of the composite polypeptide and the plant extract, stir evenly, add the preservative, continue to stir evenly, and degas in vacuum to obtain the product.

[0135] Example 2 The difference between this embodiment and embodiment 1 is that the active polypeptides are acetyl hexapeptide-8, palmitoyl tripeptide-1, snake venom peptide, and acetyl octapeptide-3, and the mass ratio is 4:2:1:1.

[0136] Comparative Example 1 The difference between this comparative example and Example 1 is that the active polypeptides are acetyl hexapeptide-8, palmitoyl tripeptide-1, and acetyl octapeptide-3, and the mass ratio is 6:5:2.

[0137] Comparative Example 2 The difference between this comparative example and Example 1 is that the raw materials for preparing the anti-wrinkle, firming and moisturizing cosmetics are, by weight percentage, 6.0% active polypeptide, 2.5% plant extract, 8% moisturizer, 3% emulsifier, 12.5% ​​emollient, 0.2% preservative, and the balance is supplemented with water to 100%.

[0138] The active polypeptides are acetyl hexapeptide-8, palmitoyl tripeptide-1, snake venom peptide, and acetyl octapeptide-3, with a mass ratio of 6:5:3:2.

[0139] Comparative Example 3 The difference between this comparative example and Example 1 is that the plant extract is Eryngium littoralis extract.

[0140] Comparative Example 4 The difference between this comparative example and Example 1 is that the moisturizing agent is medium molecular weight hyaluronic acid, sodium polyglutamate and glycerin, and the mass ratio is 3:2:3.

[0141] Comparative Example 5 The difference between this comparative example and Example 1 is that the preparation method of the hyaluronic acid composite comprises the following steps: mixing macromolecular hyaluronic acid, medium molecular hyaluronic acid, small molecular hyaluronic acid and deionized water, homogenizing at 500 bar for 5 minutes, and then removing the deionized water by rotary evaporation to obtain the composite.

[0142] The mass ratio of the large molecular hyaluronic acid, the medium molecular hyaluronic acid and the small molecular hyaluronic acid is 5:6:4.

[0143] The added amount of the deionized water is 10 times the total mass of the large molecular hyaluronic acid, the medium molecular hyaluronic acid, and the small molecular hyaluronic acid.

[0144] Comparative Example 6 The difference between this comparative example and Example 1 is that the emollient includes caprylic / capric triglyceride, meadowfoam seed oil, and camellia oil in a mass ratio of 5:4:3.

[0145] Comparative Example 7 The difference between this comparative example and Example 1 is that the preparation method of the camellia oil nanoliposomes comprises the following steps: mixing camellia oil, hydrogenated lecithin, co-solvent and stabilizer, ultrasonically emulsifying for 5 minutes, placing in a high-pressure reactor for supercritical carbon dioxide treatment, and homogenizing at 80 bar after decompression to obtain the nanoliposomes.

[0146] The mass ratio of the camellia oil to hydrogenated lecithin is 5:1.

[0147] Performance Testing ① Cosmetic stability test: Refer to the "Technical Specifications for Safety of Cosmetics" (2022 edition) to conduct high temperature, low temperature and centrifugation tests on cosmetics to determine the stability of cosmetics. High temperature test: Place the cosmetic sample in a (40±1)℃ constant temperature box for 30 days to observe whether there is stratification, discoloration, or precipitation. If not, it is qualified. Low temperature test: Freeze the cosmetic sample at (-15±1)℃ for 24 hours, and observe whether there is stratification, discoloration, or precipitation after returning to room temperature. If not, it is qualified. Centrifugation test: Centrifuge the cosmetic sample at 3000r / min for 30 minutes to check whether there is stratification or precipitation. If not, it is qualified. The results are shown in Table 1.

[0148] Table 1 Measurement results

[0149] According to statistics, the anti-wrinkle, firming and moisturizing cosmetics prepared in Examples 1-2 of the present invention are qualified in high and low temperature and centrifugation tests, indicating that the cosmetics prepared by the present invention have excellent stability. Comparative Example 1 does not add snake venom peptides, Comparative Example 2 does not freeze-dry the active polypeptides, Comparative Example 3 does not add Centella asiatica extract and ginseng extract, Comparative Example 4 uses medium molecular hyaluronic acid to replace the hyaluronic acid complex, Comparative Example 5 directly mixes three molecular weight hyaluronic acids to prepare a hyaluronic acid complex, Comparative Example 6 does not perform nanoliposome treatment on camellia oil, and Comparative Example 7 does not add cholesterol. The stability of each cosmetic has defects.

[0150] ②Efficacy test 90 female volunteers aged 25-55 were selected, with rough and dark skin, lack of elasticity, crow's feet and wrinkles under the eyes, and no skin diseases. The volunteers were divided into 9 groups, and the samples of Examples 1-2 and Comparative Examples 1-7 were tried respectively, and used once in the morning and evening according to daily habits. After one month of trial, the transepidermal water loss rate of the volunteers' faces before and after the trial was tested using a transepidermal water loss rate tester. The transepidermal water loss rate change rate = (transepidermal water loss rate after trial - transepidermal water loss rate before trial) / transepidermal water loss rate before trial × 100%. Since the transepidermal water loss rate after the trial is smaller, the general change rate is negative. The more negative the value, the better the moisturizing effect and the less the transepidermal water loss rate. The average value is taken, and the results are shown in Table 2.

[0151] PrimosCR was used to take 3D images of the local skin at the corner of the subject's eye, and then the crow's feet area was analyzed using professional analysis software. After one month of trial, the crow's feet area change rate = (crow's feet area after trial - crow's feet area before trial) / crow's feet area before trial × 100%. Since the crow's feet area is smaller after the trial, the change rate is generally negative. The more negative the value, the better the anti-wrinkle effect and the fewer skin wrinkles. The average value was taken, and the results are shown in Table 2.

[0152] Table 2 Measurement results

[0153] According to statistics, the anti-wrinkle, firming and moisturizing cosmetics prepared by Examples 1-2 of the present invention have negative values ​​for the rate of change of epidermal water loss rate and the rate of change of crow's feet area, and the absolute values ​​are relatively high, indicating that skin wrinkles are reduced and moisturizing performance is increased after the cosmetics are tried. Comparative Example 1 does not add snake venom peptides, Comparative Example 2 does not freeze-dry the active polypeptides, Comparative Example 3 does not add Centella asiatica extract and ginseng extract, Comparative Example 4 replaces the hyaluronic acid complex with medium molecular hyaluronic acid, Comparative Example 5 directly mixes three molecular weight hyaluronic acids to prepare hyaluronic acid complexes, Comparative Example 6 does not perform nanoliposome treatment on camellia oil, and Comparative Example 7 does not add cholesterol. The anti-wrinkle effect and moisturizing effect of the prepared cosmetics after trial are poor, but still better than before trial. Therefore, the cosmetics prepared by the raw materials and methods described in this application can not only achieve anti-wrinkle, firming and moisturizing effects at the same time, but also have good use experience and high stability.

[0154] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. An anti-wrinkle, firming and moisturizing cosmetic, characterized in that: Calculated by weight percentage, it includes 5.5%-6.5% of compound polypeptide freeze-dried powder, 2%-3% of plant extract, 7%-9% of moisturizer, 2.5%-3.5% of emulsifier, 10%-15% of emollient, 0.1%-0.3% of preservative, and water is added to make up the balance to 100%; The preparation method of the composite polypeptide lyophilized powder comprises the following steps: mixing an active polypeptide, a polypeptide carrier and a protective agent, stirring until completely dissolved, homogenizing, and lyophilizing to obtain the composite polypeptide; the active polypeptides are acetyl hexapeptide-8, palmitoyl tripeptide-1, snake venom peptide, and acetyl octapeptide-3, and the mass ratio is (2-4): (2-3): (1-2):

1.

2. The anti-wrinkle, firming and moisturizing cosmetic according to claim 1, characterized in that: The polypeptide carrier is a β-glucan aqueous solution and hyaluronic acid nanospheres, with a mass ratio of (4-6):

1.

3. The anti-wrinkle, firming and moisturizing cosmetic according to claim 1, characterized in that: The plant extracts are Eryngium maritima extract, Centella asiatica extract and Ginseng extract, with a mass ratio of 1: (1.3-1.8): (1.5-2.5).

4. The anti-wrinkle, firming and moisturizing cosmetic according to claim 3, characterized in that: The preparation method of the Eryngium littoralis extract comprises the following steps: A1. Take the stems and leaves of Eryngium littoralis, dry them separately and crush them to obtain pretreated Eryngium littoralis; A2, activating the fermentation agent to obtain an activation solution; after uniformly mixing the pretreated Eryngium marinum and glucose, adding water to adjust the water content to obtain a fermentation substrate, inoculating the activation solution and fermenting at 25-40° C. for 40-100 hours to obtain a fermentation product; A3. Mix the fermentation product with deionized water, perform ultrasonic water extraction and centrifugation, and filter the supernatant through an ultrafiltration membrane to obtain a retained macromolecular protein with a molecular weight of >10 kDa. After dialyzing with pure water, the supernatant is spray-dried to obtain the product.

5. The anti-wrinkle, firming and moisturizing cosmetic according to claim 1, characterized in that: The moisturizing agent is hyaluronic acid complex, sodium polyglutamate and glycerin.

6. The anti-wrinkle, firming and moisturizing cosmetic according to claim 5, characterized in that: The preparation method of the hyaluronic acid composite comprises the following steps: respectively adding deionized water to macromolecular hyaluronic acid, medium molecular hyaluronic acid and small molecular hyaluronic acid to prepare macromolecular hyaluronic acid gel, medium molecular hyaluronic acid gel and small molecular hyaluronic acid gel, then pouring the medium molecular hyaluronic acid gel into the small molecular hyaluronic acid gel for homogenization, pouring the macromolecular hyaluronic acid gel into the gel for homogenization to obtain a composite gel, and freeze-drying the composite gel to remove the deionized water.

7. The anti-wrinkle, firming and moisturizing cosmetic according to claim 6, characterized in that: The molecular weight of the large molecule hyaluronic acid is 1500k-2500kDa; the molecular weight of the medium molecule hyaluronic acid is 40k-100kDa; and the molecular weight of the small molecule hyaluronic acid is 1k-10kDa.

8. The anti-wrinkle, firming and moisturizing cosmetic according to claim 1, characterized in that: The emollient comprises caprylic / capric triglyceride, meadowfoam seed oil, and camellia oil nanoliposomes, with a mass ratio of (1.5-2): (1-1.5):

1.

9. The anti-wrinkle, firming and moisturizing cosmetic according to claim 8, characterized in that: The preparation method of camellia oil nanoliposomes comprises the following steps: mixing camellia oil, hydrogenated lecithin, cholesterol, co-solvent and stabilizer, ultrasonically emulsifying for 5 minutes, placing in a high-pressure reactor for supercritical carbon dioxide treatment, and homogenizing after decompression to obtain the nanoliposomes.

10. A method for preparing the anti-wrinkle, firming and moisturizing cosmetic according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1. Preparation of aqueous phase: mixing the moisturizer and water, and stirring until completely dissolved to obtain an aqueous phase; S2, oil phase preparation: after mixing the emollients, add the emulsifier and stir evenly to obtain the oil phase; S3. Homogenization and emulsification: After heating the water phase and the oil phase separately, pour the oil phase into the water phase, homogenize in a homogenizer, cool down, add the compound polypeptide freeze-dried powder and the plant extract, stir evenly, add the preservative, continue to stir evenly, and vacuum degas.

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