Application of rabdosia lophanthide in preparation of products for preventing skin aging

Abstract

The invention relates to the technical field of food or medicine, and particularly provides a novel application of rabdosia lophanthide in preparation of a product for preventing skin aging. Experimental evaluation of D-galactose induced aged mice proves that the serrate rabdosia herb can significantly improve the damaged condition of skin cuticle, significantly increase the skin epidermis thickness, significantly improve the damaged condition of skin collagen structure and significantly increase the skin collagen density. Therefore, the rabdosia lophanthide can be applied to preparation of products for preventing skin aging.

Description

Technical Field

[0001] The invention relates to the technical field of food or medicine, and specifically discloses use of hyssopus chinensis in preparing a product for preventing skin aging. Background Art

[0002] As the largest organ exposed to the external environment, the aging of the skin is affected by both internal and external factors. Internal aging, also known as natural aging, is an inevitable physiological degeneration process, which is manifested in the gradual decrease in the renewal rate of epidermal cells on the one hand and the aging of fibroblasts in the dermis on the other hand. External aging mainly refers to photoaging, which is caused by external environmental factors such as light, smoking, and air pollution. Both internal and external factors will cause changes in the external morphology of the skin, a decrease in structural integrity, and a decline in physiological functions.

[0003] Xihuangcao, also known as bear gall grass and yellow juice grass, is a plant of the genus Camellia in the Lamiaceae family. It is named because it likes to grow beside the wet streams in the valleys and fresh leaves have yellow juice when crushed. Xihuangcao is a commonly used Chinese herbal medicine in the folk. It contains terpenes, phenols, phenylpropanoids, sterols and other ingredients, and has antioxidant, antibacterial, anti-inflammatory and anti-tumor activities. Existing studies have shown that Xihuangcao extracts have strong inhibitory effects on bacteria and fungi such as Staphylococcus aureus, Candida albicans, Saccharomyces cerevisiae, Bacillus subtilis, Bacillus cereus, Listeria and Shigella dysenteriae. Xihuangcao also has the effect of preventing the occurrence of cancer. Among them, Xihuangcao A is a component that has been found to have an extremely obvious inhibitory effect on the proliferation of human cervical cancer Hela cells. However, there are no reports on the preventive effect of Xihuangcao on skin aging. Summary of the invention

[0004] The purpose of the present invention is to provide the use of Herba Cynoglossi in preparing food, health care products or medicines, and specifically, to provide the use of Herba Cynoglossi in preparing products for preventing skin aging.

[0005] According to an embodiment of the present invention, the food, health product or medicine is used to prevent skin aging. The inventor found that Herba Cynoglossi can improve the damage of the skin stratum corneum, increase the thickness of the skin epidermis, improve the damage of the skin collagen structure, and increase the skin collagen density, which helps to prevent skin aging.

[0006] According to an embodiment of the present invention, the skin aging is selected from skin aging induced by D-galactose. The inventors found that Herba Lycopodii has a good preventive effect on skin aging induced by D-galactose.

[0007] According to an embodiment of the present invention, the food or medicine is used for at least one of the following: improving the damage of the skin stratum corneum, increasing the thickness of the skin epidermis, improving the damage of the skin collagen structure, and increasing the skin collagen density.

[0008] According to an embodiment of the present invention, the food, health product or medicine further contains food or pharmaceutically acceptable excipients.

[0009] According to an embodiment of the present invention, the dosage form of the food, health product or medicine is selected from tablets, liquids, powdered granules, chewable tablets or soft gels.

[0010] Compared with the prior art, the present invention has the following beneficial effects:

[0011] The present invention has discovered a new use of radix serrata. Animal experimental results show that intervention with 0.5% and 1% (w / w) radix serrata leaf powder for 8 weeks can significantly improve the damage to the skin stratum corneum, significantly increase the thickness of the skin epidermis, significantly improve the damage to the skin collagen structure, and significantly increase the skin collagen density, indicating that radix serrata can prevent skin aging. The present invention provides a new use of radix serrata in the preparation of related foods, health products and medicines that have the effect of preventing D-galactose-induced skin aging. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 Effects of Herba Lycopodii on body weight in aging mice.

[0013] Figure 2 Effect of Herba Lycopodii on food intake in aging mice.

[0014] Figure 3 The figure shows the effect of Herba Cynoglossi on the organ indexes of aging mice; A is the liver coefficient, from left to right are Chow group, D-gal group, HIL-L group and HIL-H group, B is the kidney coefficient, and C is the spleen coefficient. * p<0.05.

[0015] Figure 4 These are the results of H&E staining of skin tissue sections of four groups of mice; from left to right they are Chow group, D-gal group, HIL-L group and HIL-H group, and the red arrow indicates the epidermis.

[0016] Figure 5 Effects of Herba Lycopodii on epidermal thickness of skin tissue in aging mice. **** p<0.0001.

[0017] Figure 6 These are the results of Masson staining sections of mouse skin tissue in four groups; from left to right they are Chow group, D-gal group, HIL-L group and HIL-H group, and the red circle is the dermal collagen part.

[0018] Figure 7 Effects of Herba Lycopodii on collagen density in skin tissue of aging mice. * p<0.05, ***p<0.001.

[0019] Note: The present invention Figures 1 to 7 middle:

[0020] Chow represented the control group, which was fed with normal diet;

[0021] D-gal represents the model group, which was fed with normal feed;

[0022] HIL-L represents the low-dose cyperus rotundus intervention group, which was fed with a feed containing 0.5% (w / w) cyperus rotundus leaf powder.

[0023] HIL-H represents the high-dose hyssop intervention group, which was fed a diet containing 1% (w / w) hyssop leaf powder. DETAILED DESCRIPTION

[0024] The present invention is further described below in conjunction with the embodiments of the present invention and the accompanying drawings, but the protection scope of the present invention is not limited thereto.

[0025] Example 1

[0026] Effects of Herba Cynanchifoliae on Body Weight and Food Intake of D-galactose-induced Aging Mice

[0027] Seven-week-old healthy male KM mice were selected and raised in a specific pathogen-free (SPF) environment (ambient temperature 22±2℃, relative humidity 40-70%, 12h-12h light-dark cycle). After 1 week of adaptive feeding, the mice were randomly divided into a control group, a model group, a low-dose cyperus rotundus intervention group, and a high-dose cyperus rotundus intervention group, with 10 mice in each group. The control group was subcutaneously injected with normal saline in the neck and back every day and fed with ordinary feed; the model group was subcutaneously injected with 500mg / kg D-galactose in the neck and back every day and fed with ordinary feed; the low-dose cyperus rotundus intervention group was subcutaneously injected with 500mg / kg D-galactose in the neck and back every day and fed with feed containing 0.5% cyperus rotundus leaf powder; the high-dose cyperus rotundus intervention group was subcutaneously injected with 500mg / kg D-galactose in the neck and back every day and fed with feed containing 1% cyperus rotundus leaf powder. The experimental period was 8 weeks, during which all mice had free access to food and water, and the average food intake of mice was recorded daily, and the weight of mice was recorded weekly.

[0028] according to Figure 1 , 2 As shown, compared with the model group, low-dose and high-dose intervention with Rhizoma Cyperi had no significant effect on the body weight and food intake of aging mice.

[0029] Example 2

[0030] Effects of Herba Cynanchifoliae on Organ Indexes of D-galactose-induced Aging Mice

[0031] The mice fed for 8 weeks in Example 1 were fasted for 6 hours (8:00-14:00), and blood was collected from the eye sockets and then the mice were killed by cervical dislocation. The liver, kidney and spleen of the mice were dissected and the organ coefficient was determined. Organ coefficient = organ weight (g) / mouse body weight (g).

[0032] according to Figure 3 As shown, compared with the control group, there were no significant differences in the liver coefficient, kidney coefficient and spleen coefficient of mice in the cyperus rotundus intervention group, indicating that cyperus rotundus was not found to have toxic effects on mice.

[0033] Example 3

[0034] Effects of Herba Cynanchifoliae on skin integrity and epidermal thickness of skin tissue in D-galactose-induced aging mice

[0035] The mice fed for 8 weeks in Example 1 were fasted for 6h (8:00-14:00), and the mice were killed by cervical dislocation after blood was collected from the orbits. The skin tissue of the mice was fixed in 10% formalin fixative after depilation of the back. When H&E staining was performed, the fixed mouse skin tissue was placed in a mold, dehydrated with ethanol, transparentized with xylene and embedded in paraffin; the embedded tissue was sliced ​​with a thickness of 5μm and dried in an oven at 45°C overnight; the slices were taken out and dewaxed with xylene twice, then gradient hydrated with ethanol solution, and washed with double distilled water; then incubated with hematoxylin stain for 5min, rinsed with distilled water, rinsed with 5% ethanol, incubated with eosin stain for 30s, dehydrated with 95% ethanol twice, transparentized with xylene twice, sealed with neutral gum, and scanned and observed with a pathological section scanning instrument. ImageJ (National Institute of Health, Bethesda, MD, USA) software was used to measure the epidermal thickness of the skin tissue of the H&E stained slices and statistically analyzed.

[0036] according to Figure 4 , Figure 5 As shown, compared with the control group, the skin stratum corneum of the mice in the model group became thinner or even fell off, and the epidermal thickness was significantly reduced, indicating that the skin integrity of the aging mice was significantly damaged; after the intervention of Xihuangcao, the skin damage of the mice was significantly improved, and the epidermal thickness was significantly increased, indicating that Xihuangcao has the effect of improving D-galactose-induced skin integrity damage and reduced epidermal thickness.

[0037] Example 4

[0038] Effects of Herba Cyperi on collagen structure and density in skin tissue of aging mice induced by D-galactose

[0039] The mice fed for 8 weeks in Example 1 were fasted for 6 hours (8:00-14:00), and the mice were killed by cervical dislocation after blood was collected from the eye sockets. The skin tissue of the mice was fixed in 10% formalin fixative after depilation of the back. When performing Masson staining, the fixed mouse skin tissue was placed in a mold, dehydrated with ethanol, transparentized with xylene and embedded in paraffin; the embedded tissue was sliced ​​with a thickness of 5 μm and dried in an oven at 45°C overnight; the slices were taken out and stained with hematoxylin for 5 minutes, rinsed with tap water, differentiated with 1% hydrochloric acid alcohol for a few seconds, rinsed with tap water, and rinsed with running water for a few minutes to return to blue; dyed with Ponceau acid fuchsin solution for 5-10 minutes, and quickly rinsed with distilled water; then treated with phosphomolybdic acid aqueous solution for about 3-5 minutes, and directly counterstained with aniline blue solution for 5 minutes; then treated with 1% glacial acetic acid for 1 minute, and finally dehydrated and sealed, and examined under a microscope and collected images. ImageJ (National Institute of Health, Bethesda, MD, USA) software was used to measure the collagen density of skin tissue in Masson-stained sections and conduct statistical analysis.

[0040] according to Figure 6 , Figure 7 As shown, compared with the control group, the skin collagen structure of the mice in the model group was sparse, loose, slender, irregular and even broken, and the collagen density tended to decrease, indicating that the skin collagen structure of aging mice was damaged; after the intervention of Xihuangcao, the damage of the collagen structure of the mouse skin was significantly improved, and the collagen density was significantly increased, indicating that Xihuangcao has the effect of improving D-galactose-induced skin tissue collagen structure damage and reduced collagen density.

[0041] The present invention is not limited to the above-mentioned embodiments. Without departing from the essential content of the present invention, any deformation, improvement and substitution that can be thought of by those skilled in the art shall fall within the scope of the present invention.

Claims

1. The use of Herba Cynanchifoliae in preparing food, health products or medicines, characterized in that: The food, health product or medicine is used for preventing skin aging.

2. The use according to claim 1, wherein the skin aging is selected from skin aging induced by D-galactose.

3. The use according to claim 1, characterized in that The food, health product or medicine is used for at least one of the following: Improve the damage of skin stratum corneum, increase the thickness of skin epidermis, improve the damage of skin collagen structure, and increase skin collagen density.

4. The use according to claim 1, characterized in that The food, health product or medicine also contains food or pharmaceutically acceptable auxiliary materials.

5. The use according to claim 1, characterized in that: The dosage form of the food, health product or medicine is selected from tablets, liquid, powder, chewable tablets or soft gel.

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