Filtering and purifying device for preparing cell culture medium

Abstract

The invention discloses a filtering and purifying device for preparing a cell culture medium, and relates to the technical field of biotechnology and biopharmacy. The filtering and purifying device for preparing the cell culture medium comprises a discharging pipe, a filtering mechanism is arranged on the outer side of the discharging pipe and comprises a liquid inlet pipe, the liquid inlet pipe is connected with a flange of the discharging pipe, a pre-filtering pipe is fixedly connected to the inner side of the liquid inlet pipe, one end of the pre-filtering pipe is fixedly connected with an activated carbon adsorption pipe, and the other end of the pre-filtering pipe is fixedly connected with a water inlet pipe. One end of the activated carbon adsorption tube is fixedly connected with a microporous filter tube; a multi-stage filtering system is composed of a pre-filtering pipe, an activated carbon adsorption pipe, a microporous filtering pipe, a degerming-stage filtering pipe and an ultrafiltration pipe, multi-stage filtering purification including pre-filtering, adsorption, microporous filtering, degerming-stage filtering, ultrafiltration, ion exchange and ultraviolet disinfection can be achieved through the arrangement of a filtering mechanism, it is ensured that various impurities and microorganisms are removed, and the purification effect is good. The culture medium quality is improved.

Description

Technical Field

[0001] The invention relates to the technical field of biotechnology and biopharmaceuticals, and in particular to a filtering and purifying device for preparing a cell culture medium. Background Art

[0002] The preparation of cell culture medium is one of the key steps in biomedical research and biopharmaceutical production. In order to ensure that cells can grow normally in the culture medium, the culture medium must be sterile and free of any impurities that may affect cell growth or experimental results. Filtration purification devices play an important role in the preparation of cell culture medium, which can effectively remove microorganisms, particulate matter and other contaminants.

[0003] However, the existing culture medium filtration and purification device has relatively weak filtration and purification capabilities and can only remove some tiny particles. It is difficult to effectively filter and purify bacteria and microorganisms. There are certain defects in the preparation of the culture medium, which can easily affect the preparation quality of the culture medium to a certain extent. Summary of the invention

[0004] In view of the shortcomings of the prior art, the present invention provides a filtering and purifying device for preparing a cell culture medium, which solves the problem that the prior art is difficult to effectively filter and purify bacteria and microorganisms.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions: a filtration and purification device for preparing cell culture medium, comprising a discharge pipe, a filtration mechanism is arranged on the outside of the discharge pipe, the filtration mechanism comprises a liquid inlet pipe, the liquid inlet pipe is flange-connected to the discharge pipe, a pre-filtration tube is fixedly connected to the inner side of the liquid inlet pipe, one end of the pre-filtration tube is fixedly connected to an activated carbon adsorption tube, one end of the activated carbon adsorption tube is fixedly connected to a microporous filtration tube, one end of the microporous filtration tube is fixedly connected to a sterilizing-grade filtration tube, one end of the sterilizing-grade filtration tube is fixedly connected to an ultrafiltration tube, one end of the ultrafiltration tube is fixedly connected to an ion exchange resin filtration tube, one end of the ion exchange resin filtration tube is fixedly connected to an ultraviolet disinfection tube, the outer end of the ultraviolet disinfection tube is fixedly connected to a liquid discharge pipe, and a quantity control valve is arranged on the surface of the liquid discharge pipe.

[0006] Furthermore, a shield tube is fixedly installed on the outer side of the filter mechanism, and a fixed shield tube is fixedly installed on the top end of the shield tube.

[0007] Furthermore, a pump is fixedly installed on the inner side of the fixed shield tube, the output end of the pump is fixedly connected to the liquid inlet pipe, and a support seat plate is fixedly installed on the bottom of the shield tube.

[0008] Furthermore, the discharge pipe is fixedly installed at the bottom of the reaction cylinder, and a driving motor is fixedly installed at the bottom of the reaction cylinder.

[0009] Furthermore, a mixing plate is fixedly mounted on the output end of the driving motor, and a stirring rod is fixedly mounted on the surface of the mixing plate.

[0010] Furthermore, a heating plate is fixedly installed on the inner surface of the reaction cylinder, and an electric heater is fixedly installed on the outer side of the reaction cylinder, and the electric heater is electrically connected to the heating plate.

[0011] Furthermore, a control panel is fixedly mounted on the front of the reaction cylinder, and operation buttons are arranged on the surface of the control panel.

[0012] Furthermore, a support leg is fixedly installed at the bottom of the reaction cylinder, and a sealing cover plate is bolted to the top of the reaction cylinder.

[0013] Furthermore, a dry material inlet hopper is fixedly mounted on the surface of the sealing cover plate, and a wet material inlet hopper is fixedly mounted on one side of the dry material inlet hopper.

[0014] Furthermore, a screen plate is movably installed on the inner side of the wet material inlet hopper, and covers are threadedly installed on the tops of the dry material inlet hopper and the wet material inlet hopper.

[0015] The present invention has the following beneficial effects:

[0016] 1. The filtering and purification device for preparing cell culture medium comprises a multi-stage filtering system composed of a pre-filter tube, an activated carbon adsorption tube, a microporous filter tube, a sterilizing-grade filter tube and an ultrafiltration tube, wherein the outermost layer of the pre-filter tube is a coarse fiber net or foam material, and the middle layer is a finer non-woven fabric or fiber filter paper to protect subsequent finer filtering units from being blocked or damaged; the activated carbon adsorption tube is composed of multiple layers of activated carbon particles or activated carbon fibers to adsorb and remove organic pollutants in water; the core of the microporous filter tube is a microporous filter membrane with a pore size range of . to . microns, which can effectively block bacteria, fungal spores and other microorganisms while allowing nutrients to pass smoothly to ensure the sterility of the culture medium.

[0017] 2. The filtration and purification device for preparing cell culture medium is similar to a microporous filter tube in interior through the setting of a sterilizing-grade filter tube, but uses a double-layer or multi-layer filter membrane combination and adds hydrophobic materials to prevent the filter membrane from wetting, which can provide a higher level of microbial barrier and ensure the complete removal of bacteria and other microorganisms. An ultrafiltration membrane is arranged inside the ultrafiltration tube, which is a semipermeable membrane with a pore size between . to . microns, which can not only remove microorganisms, but also concentrate or separate components within a specific molecular weight range, such as proteins, polysaccharides, etc., thereby optimizing the composition of the culture medium.

[0018] 3. The filtering and purification device for preparing cell culture medium is provided with an ion exchange resin filter tube, which is filled with cation and / or anion exchange resin beads. The surface of these beads has exchangeable ion groups, which can be used to remove specific ions or adjust the pH value, optimize the chemical composition of the culture medium, and ensure the optimal conditions for cell growth. The ultraviolet disinfection tube includes one or more ultraviolet lamp tubes installed in a reflective cover. A transparent quartz sleeve is provided around the lamp tube to prevent the water flow from directly contacting the lamp tube. It can use high-intensity ultraviolet rays to destroy the DNA / RNA structure of microorganisms and achieve a highly efficient sterilization effect, which is particularly suitable for heat-sensitive components.

[0019] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a front view stereoscopic schematic diagram of the overall structure of the present invention;

[0021] Figure 2 It is a bottom-up stereoscopic schematic diagram of the overall structure of the present invention;

[0022] Figure 3 It is a partially enlarged three-dimensional schematic diagram of the sealing cover plate structure of the present invention;

[0023] Figure 4 It is an enlarged three-dimensional schematic diagram of the internal section of the reaction cylinder structure of the present invention;

[0024] Figure 5 It is a partially enlarged three-dimensional schematic diagram of the shield tube structure of the present invention;

[0025] Figure 6 It is a partially enlarged three-dimensional schematic diagram of the filter mechanism structure of the present invention;

[0026] Figure 7 It is a partially cutaway enlarged stereoscopic schematic diagram of the filter mechanism structure of the present invention;

[0027] In the figure, 1. discharge pipe; 2. filtering mechanism; 201. liquid inlet pipe; 202. pre-filter tube; 203. activated carbon adsorption tube; 204. microporous filter tube; 205. sterilization grade filter tube; 206. ultrafiltration tube; 207. ion exchange resin filter tube; 208. ultraviolet disinfection tube; 209. liquid discharge pipe; 3. quantity control valve; 4. shield tube; 5. fixed shield tube; 6. pump; 7. support seat plate; 8. reaction cylinder; 9. drive motor; 10. mixing plate; 11. stirring rod; 12. heating plate; 13. electric heater; 14. control panel; 15. operation button; 16. support leg; 17. sealing cover plate; 18. dry material inlet hopper; 19. wet material inlet hopper; 20. sieve plate; 21. cover. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] In the description of the present invention, it should be understood that the terms "opening", "upper", "lower", "thickness", "top", "middle", "length", "inside", "all around" and the like indicating orientation or positional relationship are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the components or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0030] See also Figure 1-Figure 7 The embodiment of the present invention provides a technical solution: a filtering and purifying device for preparing a cell culture medium, comprising a discharge pipe 1, a filtering mechanism 2 is arranged on the outer side of the discharge pipe 1, the filtering mechanism 2 comprises a liquid inlet pipe 201, the liquid inlet pipe 201 is flange-connected to the discharge pipe 1, a pre-filtering pipe 202 is fixedly connected to the inner side of the liquid inlet pipe 201, one end of the pre-filtering pipe 202 is fixedly connected to an activated carbon adsorption tube 203, one end of the activated carbon adsorption tube 203 is fixedly connected to a microporous filtering tube 204, one end of the microporous filtering tube 204 is fixedly connected to a sterilizing grade filtering tube 205, and the sterilizing grade filtering tube 206 is fixedly connected to the sterilizing grade filtering tube 207. One end of 05 is fixedly connected to an ultrafiltration tube 206, one end of the ultrafiltration tube 206 is fixedly connected to an ion exchange resin filter tube 207, one end of the ion exchange resin filter tube 207 is fixedly connected to an ultraviolet disinfection tube 208, the outer end of the ultraviolet disinfection tube 208 is fixedly connected to a liquid outlet pipe 209, and a control valve 3 is arranged on the surface of the liquid outlet pipe 209; through the arrangement of the filtering mechanism 2, multi-stage filtration and purification can be achieved, including pre-filtration, adsorption, microporous filtration, sterilization-grade filtration, ultrafiltration, ion exchange and ultraviolet disinfection, to ensure the removal of various impurities and microorganisms and improve the quality of the culture medium.

[0031] Specifically, a shield tube 4 is fixedly installed on the outer side of the filter mechanism 2 , and a fixed shield tube 5 is fixedly installed on the top end of the shield tube 4 .

[0032] In this embodiment, by setting the protective cover tube 4, the filter mechanism 2 can be protected from external pollution and mechanical damage, and the internal environment can be kept clean and stable. By setting the fixed cover tube 5, the pump 6 can be fixed and supported to ensure its stable operation.

[0033] Specifically, a pump 6 is fixedly installed on the inner side of the fixed shield pipe 5 , the output end of the pump 6 is fixedly connected to the liquid inlet pipe 201 , and a support seat plate 7 is fixedly installed on the bottom of the shield pipe 4 .

[0034] In this embodiment, the pump 6 is provided to ensure that the liquid passes through each filter unit at an appropriate flow rate to maintain a stable filtering pressure, and the support seat plate 7 is provided to stably support the shield tube 4 and its internal components.

[0035] Specifically, the discharge pipe 1 is fixedly installed at the bottom of the reaction cylinder 8 , and a driving motor 9 is fixedly installed at the bottom of the reaction cylinder 8 .

[0036] In this embodiment, the setting of the reaction cylinder 8 can provide a closed and controllable environment for mixing and heating the raw materials. The setting of the driving motor 9 can provide power for the mixing plate 10 to ensure uniform mixing of the dry and wet raw materials and promote dissolution and dispersion.

[0037] Specifically, a mixing plate 10 is fixedly mounted on the output end of the driving motor 9 , and a stirring rod 11 is fixedly mounted on the surface of the mixing plate 10 .

[0038] In this embodiment, the mixing plate 10 can work in coordination with the stirring rod 11 to further enhance the mixing effect, ensure that the raw materials are fully mixed, and improve the dissolution efficiency.

[0039] Specifically, a heating plate 12 is fixedly installed on the inner surface of the reaction cylinder 8 , and an electric heater 13 is fixedly installed on the outer side of the reaction cylinder 8 . The electric heater 13 is electrically connected to the heating plate 12 .

[0040] In this embodiment, the dissolution process of the insoluble substance can be accelerated by the setting of the heating plate 12, and the electric heater 13 can provide power support for the heating plate 12 to ensure the accuracy and stability of temperature control.

[0041] Specifically, a control panel 14 is fixedly mounted on the front of the reaction cylinder 8 , and operation buttons 15 are arranged on the surface of the control panel 14 .

[0042] In this embodiment, automatic control can be achieved by setting the control panel 14 to monitor key parameters such as temperature and pH value, and manual operation and parameter adjustment can be conveniently performed by the user by setting the operation button 15.

[0043] Specifically, a support leg 16 is fixedly installed on the bottom of the reaction cylinder 8 , and a sealing cover plate 17 is bolted to the top of the reaction cylinder 8 .

[0044] In this embodiment, the support legs 16 are provided to stably support the reaction cylinder 8, thereby ensuring the structural stability and safety of the entire device. The sealing cover 17 is provided to provide a closed environment, thereby preventing external contamination from entering and ensuring the sterility of the entire preparation process.

[0045] Specifically, a dry material inlet hopper 18 is fixedly mounted on the surface of the sealing cover plate 17 , and a wet material inlet hopper 19 is fixedly mounted on one side of the dry material inlet hopper 18 .

[0046] In this embodiment, dry raw materials can be easily added through the setting of the dry material hopper 18, and wet raw materials can be easily added through the setting of the wet material hopper 19, thereby ensuring the accurate delivery of raw materials and working in coordination with the dry material hopper 18 to provide a comprehensive raw material input path.

[0047] Specifically, a sieve plate 20 is movably installed on the inner side of the wet material inlet hopper 19 , and a cover 21 is threadedly installed on the top of the dry material inlet hopper 18 and the wet material inlet hopper 19 .

[0048] In this embodiment, by setting the sieve plate 20, the wet material can be preliminarily screened to remove larger particles to ensure the quality of the raw materials. By setting the cover 21, the dry material hopper 18 and the wet material hopper 19 can be protected from external contamination to keep the internal environment clean.

[0049] When in use (working), first ensure that all components (such as pump 6, heating plate 12, electric heater 13, etc.) are working properly, all joints are well sealed, and the reaction cylinder 8 and its internal components are thoroughly cleaned and disinfected to ensure a sterile environment. The required dry powder components, such as basal culture medium powder, buffer, vitamins, etc., are added through the dry material inlet 18, and liquid components, such as serum, amino acid solution, etc., are added through the wet material inlet 19. The sieve plate 20 can be used for preliminary screening of wet materials to remove larger particles. The cover 21 on the top of the dry material inlet 18 and the wet material inlet 19 is tightened to prevent external contamination; through the control panel 1 The operation button 15 starts the driving motor 9, driving the mixing plate 10 and the stirring rod 11 to rotate, ensuring that the dry and wet raw materials are fully mixed. As needed, the mixture in the reaction cylinder 8 is heated by the electric heater 13 and the heating plate 12 to accelerate the dissolution process. After the culture medium is prepared, the mixed culture medium is sucked into the liquid inlet pipe 201 by the pump 6 in the fixed cover pipe 5, and passes through the filter pipes of each level in turn. After the filtration and purification is completed, the liquid flow rate is adjusted by the control valve 3 on the surface of the liquid outlet pipe 209 to ensure stable output. The culture medium after multi-stage filtration and purification is collected in a designated container to ensure its sterility and purity.

[0050] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0051] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A filtering and purification device for preparing a cell culture medium, comprising a discharge pipe (1), characterized in that: A filtering mechanism (2) is arranged on the outside of the discharge pipe (1), and the filtering mechanism (2) comprises a liquid inlet pipe (201), the liquid inlet pipe (201) is flange-connected to the discharge pipe (1), a pre-filtering pipe (202) is fixedly connected to the inside of the liquid inlet pipe (201), one end of the pre-filtering pipe (202) is fixedly connected to an activated carbon adsorption tube (203), one end of the activated carbon adsorption tube (203) is fixedly connected to a microporous filtering tube (204), and one end of the microporous filtering tube (204) is fixedly connected to the activated carbon adsorption tube (203). One end of the sterilizing grade filter tube (205) is fixedly connected to a sterilizing grade filter tube (205), one end of the sterilizing grade filter tube (205) is fixedly connected to an ultrafiltration tube (206), one end of the ultrafiltration tube (206) is fixedly connected to an ion exchange resin filter tube (207), one end of the ion exchange resin filter tube (207) is fixedly connected to an ultraviolet disinfection tube (208), the outer end of the ultraviolet disinfection tube (208) is fixedly connected to a liquid outlet pipe (209), and a control valve (3) is provided on the surface of the liquid outlet pipe (209).

2. A filtering and purification device for preparing a cell culture medium according to claim 1, characterized in that: A shield tube (4) is fixedly mounted on the outer side of the filtering mechanism (2), and a fixed shield tube (5) is fixedly mounted on the top end of the shield tube (4).

3. A filtering and purification device for preparing a cell culture medium according to claim 2, characterized in that: A pump (6) is fixedly mounted on the inner side of the fixed shield tube (5), the output end of the pump (6) is fixedly connected to the liquid inlet pipe (201), and a support seat plate (7) is fixedly mounted on the bottom of the shield tube (4).

4. A filtering and purification device for preparing a cell culture medium according to claim 1, characterized in that: The discharge pipe (1) is fixedly mounted on the bottom of the reaction cylinder (8), and a driving motor (9) is fixedly mounted on the bottom of the reaction cylinder (8).

5. A filtering and purification device for preparing a cell culture medium according to claim 4, characterized in that: A mixing plate (10) is fixedly mounted on the output end of the driving motor (9), and a stirring rod (11) is fixedly mounted on the surface of the mixing plate (10).

6. A filtering and purifying device for preparing a cell culture medium according to claim 4, characterized in that: A heating plate (12) is fixedly mounted on the inner surface of the reaction cylinder (8), and an electric heater (13) is fixedly mounted on the outer side of the reaction cylinder (8), wherein the electric heater (13) is electrically connected to the heating plate (12).

7. A filtering and purification device for preparing a cell culture medium according to claim 4, characterized in that: A control panel (14) is fixedly mounted on the front of the reaction cylinder (8), and an operating button (15) is arranged on the surface of the control panel (14).

8. A filtering and purifying device for preparing a cell culture medium according to claim 4, characterized in that: A support leg (16) is fixedly mounted on the bottom of the reaction cylinder (8), and a sealing cover plate (17) is bolted to the top of the reaction cylinder (8).

9. A filtering and purifying device for preparing a cell culture medium according to claim 8, characterized in that: A dry material inlet hopper (18) is fixedly mounted on the surface of the sealing cover plate (17), and a wet material inlet hopper (19) is fixedly mounted on one side of the dry material inlet hopper (18).

10. A filtering and purifying device for preparing a cell culture medium according to claim 9, characterized in that: A sieve plate (20) is movably mounted on the inner side of the wet material inlet hopper (19), and covers (21) are threadedly mounted on the tops of the dry material inlet hopper (18) and the wet material inlet hopper (19).

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