Method for clarifying turbid liquid and application

Abstract

The invention discloses a method for clarifying turbid liquid and application. The clarification method provided by the invention comprises the following steps: taking an isolated turbid solution, adding a reagent, and stirring to clarify the turbid solution; the reagent is an aqueous solution containing polyvinylpyrrolidone; the turbid liquid contains blood, body fluid and normal saline. According to the invention, polyvinylpyrrolidone is added into the turbid liquid containing blood and body fluid, so that the turbid liquid can quickly become clear and transparent, and the method can ensure that the visual field of an endoscope is clear in the operation process, the operation difficulty is reduced, the operation curative effect is ensured and the operation safety coefficient is increased.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and in particular to a method for clarifying a turbid liquid containing blood or body fluid and an application of the method. Background Art

[0002] An endoscope is an optical instrument that consists of a cold light source lens, fiber optic wires, an image transmission system, a screen display system, etc. With the assistance of an endoscope and special surgical instruments, traditional surgeries that require large incisions can be completed with only minor wounds. This not only reduces the trauma of the surgery, but also increases the accuracy and safety of the surgery, allowing the patient to recover quickly.

[0003] During endoscopic operation, the wound surface usually oozes blood, and physiological saline is usually used for flushing in clinical practice to ensure a clear view of the endoscope. However, when there is a lot of bleeding or poor fluid reflux, the turbidity of physiological saline and blood and other body fluids will seriously affect the endoscope's field of view, causing difficulties in operation, which not only affects the efficacy of the operation, but also threatens the patient's life safety. Therefore, how to ensure a clear view of the endoscope during surgery has become a practical need. Summary of the invention

[0004] The purpose of the present invention is to provide a method for clarifying turbid liquid containing blood and body fluids, by which the endoscope field of view can be ensured to be clear during surgery, the difficulty of surgery can be reduced, the efficacy of surgery can be ensured, and the safety factor of surgery can be increased.

[0005] In order to achieve the above object, the technical solution adopted by the present invention is as follows: The present invention provides a method for clarifying a turbid liquid, comprising the following steps: taking an isolated turbid liquid, adding a reagent, and stirring to clarify the turbid liquid; The reagent is an aqueous solution containing polyvinyl pyrrolidone; The turbid liquid contains blood, body fluids and physiological saline.

[0006] In one embodiment, the turbid liquid can be obtained by draining it from a surgical field through a drainage device, and the clarification method does not include the steps of obtaining the turbid liquid from the body and the subsequent steps of using the clarified liquid.

[0007] In the above method, the amount of the reagent added is: 24-25 ml of the reagent is added to every 1 ml of turbid solution.

[0008] In the above method, the stirring conditions are: temperature of 18-35°C and time of 8-15s.

[0009] The present invention also provides a reagent for endoscopic surgery, comprising physiological saline and polyvinyl pyrrolidone; the mass concentration of the polyvinyl pyrrolidone is 1%.

[0010] The reagent is one or more of iodine diluent, polyvinyl pyrrolidone (PVP) solution, cross-linked polyvinyl pyrrolidone (PVP-P) solution, and povidone iodine diluent.

[0011] In the reagent, the iodine tincture diluent is an aqueous solution formed by diluting the medical iodine tincture stock solution by 50-200 times with physiological saline.

[0012] In one embodiment, the iodine tincture diluent is a solution formed by diluting the iodine tincture stock solution by 50, 100, or 200 times with normal saline.

[0013] The iodine tincture stock solution may be medical iodine tincture, the concentration of which is usually 0.5%, and the main components are polyvinyl pyrrolidone and elemental iodine.

[0014] Among the agents, the surgeries include ureteroscopic lithotripsy, transurethral bladder tumor resection, percutaneous nephrolithotomy, cystoscopy, hysteroscopy or all other endoscopic surgeries performed in aqueous media.

[0015] The present invention further provides the use of the reagent for endoscopic surgery in preparing an endoscopic clearing liquid for endoscopic surgery.

[0016] The reagent for endoscopic surgery is mixed with a turbid liquid generated during endoscopic operation to clarify the turbid liquid and form an endoscopic clarified liquid.

[0017] Compared with the prior art, the present invention has the following beneficial effects: Polyvinylpyrrolidone (PVP) is a synthetic water-soluble polymer compound with the general properties of water-soluble polymer compounds. It is widely used in the fields of medicine, food, and cosmetics. In the field of medicine, it is widely used as an excipient for pharmaceutical preparations; in addition, PVP can also be used as a colorant and X-ray contrast agent; it can be used in a variety of dosage forms such as tablets, granules, and aqueous solutions, and has the effects of detoxification, hemostasis, increasing solubility concentration, preventing peritoneal adhesion, and promoting erythrocyte sedimentation rate.

[0018] However, the present invention unexpectedly found that when an aqueous solution containing polyvinyl pyrrolidone, such as iodine diluent, is added to a turbid liquid containing blood, body fluids and other components, the turbid liquid can be quickly clarified. This discovery can be applied to endoscopic surgery to ensure a clear endoscopic field of view, solve clinical problems, improve surgical operation safety, and has promotion and application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is the appearance of iodine tincture diluted solutions at different dilution multiples.

[0020] Figure 2This is the appearance of the blood sample after adding iodine diluent of different dilution multiples.

[0021] Figure 3 This is the relationship curve between the dilution concentration of iodine tincture and the relative transmittance of blood samples.

[0022] Figure 4 It is the transmittance of blood samples after adding iodine diluents with different dilution multiples; in the figure, the horizontal axis is the blood samples added with iodine diluents with different dilution multiples, and the vertical axis is the relative value of the transmittance of each mixed solution to the transmittance of normal saline, with the transmittance of normal saline defined as 100%.

[0023] Figure 5 This is the appearance of the blood sample after adding 1% polyvinyl pyrrolidone and iodine-containing aqueous solution.

[0024] Figure 6 It is the transmittance of blood sample after adding 1% polyvinyl pyrrolidone and iodine-containing aqueous solution. DETAILED DESCRIPTION

[0025] The present invention will be further described below in conjunction with specific embodiments, but the present invention is not limited to the following embodiments.

[0026] Unless otherwise specified, the experimental methods used in the following examples are conventional methods.

[0027] Unless otherwise specified, the reagents, materials, instruments, etc. used in the following examples can be obtained from commercial sources.

[0028] Example 1: Iodine diluent for blood sample clarification The specific operations are as follows: (1) Use physiological saline to dilute the medical iodine stock solution to 10, 50, 100, or 200 times the volume to obtain iodine dilution solutions of different concentrations; Figure 1 As shown, they are 1:10 iodine solution, 1:50 iodine solution, 1:100 iodine solution, and 1:200 iodine solution.

[0029] The medical iodine stock solution (concentration 0.5%) used in this embodiment mainly contains polyvinyl pyrrolidone and elemental iodine.

[0030] (2) Obtain 5 in vitro turbid blood samples, each with a volume of 200 μL. Add physiological saline (0.9% NaCl), 1:10 povidone-iodine solution, 1:50 povidone-iodine solution, 1:100 povidone-iodine solution, and 1:200 povidone-iodine solution in sequence, with the added volume of each being 4.8 ml. The resulting mixed solutions are marked as: (A) blood sample + 0.9% NaCl, (B) blood sample + 1:10 povidone-iodine diluent, (C) blood sample + 1:50 povidone-iodine diluent, (D) blood sample + 1:100 povidone-iodine diluent, and (E) blood sample + 1:200 povidone-iodine diluent. Stir at room temperature for 15 seconds.

[0031] The transmittance test results of each mixed solution are as follows: (1) Visual observation: Figure 2 As shown, by comparing the transparency of the bottom of the cuvette and the black background of the desktop, it can be seen that the blood sample added with normal saline is still opaque, while the transmittance of the blood sample added with iodine tincture diluent increases to varying degrees, and the bottom of the cuvette and the black background of the desktop become visible. Among them, the transmittance of the blood sample added with 1:100 iodine tincture diluent increases most significantly.

[0032] (2) Spectrophotometer measurement: The transmittance of 0.9% sodium chloride (NaCl) solution is defined as 100%. The relative transmittance of the blood sample after adding iodine diluent is measured and calculated. The relationship curve between the iodine dilution concentration and the relative transmittance of the blood sample is shown in the figure below: Figure 3 As shown, the calculation formula is as follows: y = -1028.6x 2 + 100.87x + 0.3021, where: y is the relative transmittance, x is the iodine dilution concentration; The calculation results are as follows Figure 4 As shown in the figure, the transmittance of blood sample + 0.9% NaCl, blood sample + 1:10 iodine diluent, blood sample + 1:50 iodine diluent, blood sample + 1:100 iodine diluent, and blood sample + 1:200 iodine diluent are 0, 0.115, 1.331, 2.411, and 0.445, respectively. It can be seen that the iodine diluents with dilution factors of 50 and 100 can effectively improve the transmittance of turbid blood samples, among which the iodine diluent diluted 100 times has the best clarifying effect.

[0033] Example 2: Polyvinylpyrrolidone solution for blood sample clarification The specific operations are as follows: Three in vitro turbid blood samples were obtained, each with a volume of 1 ml. 24 ml of each of 1% polyvinyl pyrrolidone solution, 0.4 mol / L iodine-containing aqueous solution, and 0.9% sodium chloride (NaCl) solution were added in sequence and stirred at room temperature for 15 seconds.

[0034] The results are as follows Figure 5 , Figure 6 As shown, the transmittance of the blood sample added with 1% polyvinyl pyrrolidone solution increased, while the transmittance of the blood sample added with iodine aqueous solution did not change substantially; further measurement using a spectrophotometer, the transmittance of 0.9% sodium chloride (NaCl) solution was defined as 100%, and the transmittances of blood sample + 0.9% NaCl, blood sample + 0.4 mol / L iodine, and blood sample + 1% polyvinyl pyrrolidone solution were 0.003, 0.005, and 1.170, respectively.

[0035] Meanwhile, the present invention also attempts to use other aqueous solutions containing polyvinyl pyrrolidone for testing, and the clarification effect thereof is substantially consistent with the clarification effect of 1% polyvinyl pyrrolidone solution on blood samples.

[0036] In summary, the above experiments show that polyvinyl pyrrolidone can make turbid liquids containing blood, body fluids and other components quickly become clear and transparent. This discovery can be used to ensure a clear endoscopic field of view during endoscopic surgery, thereby reducing the difficulty of the operation, ensuring the efficacy of the operation and increasing the safety factor of the operation.

[0037] Although the present invention has been described in detail above with general descriptions and specific embodiments, it is obvious to those skilled in the art that some modifications or improvements may be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection claimed by the present invention.

Claims

1. A method for clarifying a turbid liquid, comprising the following steps: taking an isolated turbid liquid, adding a reagent, and stirring to clarify the turbid liquid; The reagent is an aqueous solution containing polyvinyl pyrrolidone; The turbid liquid contains blood, body fluids and physiological saline.

2. The clarification method according to claim 1, characterized in that The amount of the reagent added is: 24-25 ml of the reagent is added to every 1 ml of turbid liquid.

3. The clarification method according to claim 1 or 2, characterized in that: The stirring conditions are: temperature of 18-35° C., and time of 8-15 s.

4. A surgical reagent comprising physiological saline and polyvinyl pyrrolidone; the mass concentration of the polyvinyl pyrrolidone is 1%.

5. The reagent according to claim 4, characterized in that The reagent is one or more of iodine diluent, polyvinyl pyrrolidone solution, cross-linked polyvinyl pyrrolidone solution, and povidone iodine diluent.

6. The reagent according to claim 5, characterized in that The iodine tincture diluent is a water solution formed by diluting the medical iodine tincture stock solution by 50-200 times with physiological saline.

7. The reagent according to any one of claims 4 to 6, characterized in that The surgeries include ureteroscopic lithotripsy, transurethral resection of bladder tumors, percutaneous nephrolithotomy, cystoscopy, hysteroscopy or all other endoscopic surgeries performed in water media.

8. Use of the surgical reagent according to any one of claims 4 to 7 in the preparation of an endoscopic clearing fluid for endoscopic surgery.

9. The use according to claim 8, characterized in that The surgical reagent is mixed with a turbid liquid generated during endoscopic operation to clarify the turbid liquid, thereby forming an endoscope clarified liquid.

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