Preparation device and preparation method for preparing nicotinamide ribose by applying biological enzyme method

Abstract

The invention relates to the technical field of ribose preparation, in particular to a preparation device and method for preparing nicotinamide ribose by applying a biological enzyme method, the preparation device comprises a reaction kettle, an adjusting mechanism is arranged on the reaction kettle, and a pH balance mechanism is further arranged on the reaction kettle; the reaction kettle comprises a motor, a rotating rod is arranged on the motor, a stirring paddle is arranged on the rotating rod, a discharging plate is further arranged on the reaction kettle, and a handle is arranged on the discharging plate; the adjusting mechanism comprises a first piston rod, a second piston rod is arranged on the first piston rod, a first inserting rod is arranged on the second piston rod, and the first piston rod penetrates through the second piston rod. The amount of the added raw materials and the amount of the added catalyst can be respectively controlled according to needs, so that the reaction is carried out to the maximum extent, and the product nicotinamide ribose is obtained to the maximum extent.

Description

Technical Field

[0001] The present invention relates to the technical field of ribose preparation, and in particular to a preparation device and a preparation method for preparing nicotinamide ribose by using a biological enzyme method. Background Art

[0002] Nicotinamide riboside, abbreviated as NR, is a compound with multiple biological activities. It is a derivative of vitamin B3 and an important precursor of nicotinamide adenine dinucleotide. The bioenzymatic method for preparing nicotinamide riboside is a green and efficient synthesis method. It uses enzymes as catalysts and reacts under mild conditions, avoiding some shortcomings of chemical synthesis, such as harsh reaction conditions, difficulty in product separation and purification, and environmental pollution.

[0003] In the existing bio-enzymatic method for preparing nicotinamide riboside, it is difficult to adjust the reaction product and the amount of catalyst, resulting in low reaction efficiency. In addition, manual addition of materials is prone to errors, resulting in a waste of catalyst resources. Summary of the invention

[0004] The purpose of the present invention is to provide a connection between a first plug-in rod and a first piston rod and a second piston rod, and to control whether the first plug-in rod is connected to the first piston rod. The amount of raw materials added and the amount of catalyst added can be controlled separately as needed to maximize the reaction and obtain the product nicotinamide riboside to the maximum extent.

[0005] To achieve the above object, the present invention provides the following technical solutions: a preparation device and a preparation method for preparing nicotinamide riboside by using a biological enzyme method, comprising a reactor, a regulating mechanism is provided on the reactor, and a pH balance mechanism is also provided on the reactor; The reactor comprises a motor, a rotating rod is arranged on the motor, a stirring paddle is arranged on the rotating rod, a discharge plate is arranged on the reactor, and a handle is arranged on the discharge plate; The regulating mechanism comprises a first piston rod, a second piston rod is arranged on the first piston rod, a first plug rod and a third piston rod are arranged at both ends of the second piston rod, a first plug rod and a second plug rod are arranged on the second piston rod and the third piston rod, a first piston pipe is arranged on the second piston rod, a first piston block is also arranged at one end of the second piston rod, the first piston rod penetrates the first piston block and is also provided with a second piston block, a first feed barrel is arranged on the first piston pipe and located above the first piston block, a second feed barrel is arranged on the first piston pipe and located above the second piston block, and a feed pipe is also arranged on the first piston pipe; The ph balancing mechanism includes a material storage barrel, which is also provided with a water inlet pipe, a second piston pipe is provided on the water inlet pipe, a fourth piston rod is provided on the second piston pipe, one end of the fourth piston rod is fixedly connected to the third piston rod, a water inlet barrel is also provided on the water inlet pipe, and a limiting pipe is also provided on the material storage barrel, and a limiting ball is provided on the limiting pipe.

[0006] Preferably, the first piston conduit and the feed pipe are hollow in design, and their sizes are compatible with the second piston block and the first piston block.

[0007] Preferably, the second piston rod and the end of the first piston rod close to the first piston block are both provided with holes, and the size of the holes is adapted to the first plug-in rod.

[0008] Preferably, the third piston rod and the first piston rod are both provided with holes at one end close to the second piston block, and the size of the holes is adapted to the second plug-in rod.

[0009] Preferably, the second piston pipe is hollow in design and has a size that matches that of the fourth piston rod.

[0010] Preferably, the reactor is provided with a plurality of holes, the sizes of which are respectively adapted to the discharge plate and the handle.

[0011] Preferably, a scraper is provided on the stirring paddle, and the scraper is in contact with the inner wall of the reaction kettle.

[0012] Preferably, the limiting tube is hollow in design, grooves of different sizes are arranged inside the limiting tube, and the limiting ball is limited in the groove by the limiting tube.

[0013] Preferably, a support column is provided on the material storage barrel, and the material storage barrel and the water inlet pipe are both hollow in design.

[0014] A method for preparing nicotinamide riboside by a bioenzymatic method comprises the following steps: Step 1: Put nicotinamide and purine nucleoside mixture as raw materials from the first feed barrel, control the amount of raw materials entering the reactor by adjusting the second piston rod, then put enzyme solution as a catalyst for the reaction from the second feed barrel, control the amount of catalyst entering the reactor by adjusting the first piston rod, and control whether the first plug-in rod is connected to the first piston rod by plugging the first plug-in rod with the first piston rod and the second piston rod; Step 2: After the reactants and catalyst are added to the reactor, the connection between the fourth piston rod and the first piston rod is adjusted by adjusting the connection between the second plug-in rod and the third piston rod and the first piston rod, and the amount of the alkaline solution added to the water inlet barrel is adjusted by adjusting the first piston rod and the fourth piston rod, and enters the water inlet pipe and the storage barrel; Step 3: Turn on the motor to drive the rotating rod, which drives the stirring paddle to rotate, and stir the mixed liquid in the reactor. At the same time, the stirring paddle squeezes the limiting ball on the inner wall of the reactor, so that the alkaline solution in the storage barrel enters the interior of the reactor through the limiting tube, neutralizes the water produced by the reaction, and adjusts the pH value of the reaction environment.

[0015] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention controls whether the first plug-in rod is connected to the first piston rod by plugging the first plug-in rod with the first piston rod and the second piston rod. The amount of raw materials added and the amount of catalyst added can be controlled separately as needed to maximize the reaction and obtain the product nicotinamide riboside to the maximum extent.

[0016] 2. After the reaction starts, the present invention adjusts the connection between the fourth piston rod and the first piston rod by adjusting the connection between the second plug-in rod and the third piston rod and the first piston rod, and adjusts the amount of alkaline solution added to the water inlet barrel by adjusting the first piston rod and the fourth piston rod, so that the amount of alkaline solution enters the water inlet pipe and enters the material storage barrel, which is convenient for entering the reactor from the material storage barrel during the reaction, preventing the reaction from being affected by manual adjustment of the pH value during the reaction, and automatically adjusting the pH balance to ensure the normal reaction and efficiently produce nicotinamide riboside.

[0017] 3. The present invention simultaneously starts the motor to drive the rotating rod, which drives the stirring paddle to rotate, and stirs the mixed liquid in the reactor. At the same time, the stirring paddle squeezes the limiting ball on the inner wall of the reactor, so that the alkaline solution in the storage barrel enters the interior of the reactor through the limiting tube, neutralizes the water produced by the reaction, adjusts the pH value of the reaction environment, prevents the water produced by the reaction from causing the pH value of the reaction environment to change and affect the activity of the enzyme, affecting the efficiency of the reaction, and ensuring efficient reaction. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a partial structural schematic diagram of the present invention; Figure 3 For the present invention Figure 2 Enlarged view of part A in the middle; Figure 4 For the present invention Figure 2 Enlarged view of part B; Figure 5 The second schematic diagram of the partial structure of the present invention; Figure 6 The third is a partial structural schematic diagram of the present invention; Figure 7 For the present invention Figure 6 Enlarged view of part C.

[0019] In the figure: 1. reactor; 2. adjustment mechanism; 3. ph balance mechanism; 11. handle; 12. rotating rod; 13. stirring paddle; 14. scraper; 15. discharge plate; 16. motor; 21. first piston rod; 22. first piston pipe; 23. first piston block; 24. second piston block; 25. first feed barrel; 26. second feed barrel; 27. first plug-in rod; 271. second piston rod; 28. second plug-in rod; 281. third piston rod; 29. ​​feed pipe; 31. storage barrel; 32. support column; 33. water inlet pipe; 34. water inlet barrel; 35. second piston pipe; 36. fourth piston rod; 37. limiting pipe; 38. limiting ball. DETAILED DESCRIPTION

[0020] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] The present invention provides a preparation device and a preparation method for preparing nicotinamide riboside by using a biological enzyme method, comprising a reactor 1, a regulating mechanism 2 is arranged on the reactor 1, and a pH balance mechanism 3 is also arranged on the reactor 1; The reactor 1 includes a motor 16, a rotating rod 12 is provided on the motor 16, a stirring paddle 13 is provided on the rotating rod 12, and a discharge plate 15 is provided on the reactor 1, and a handle 11 is provided on the discharge plate 15; The regulating mechanism 2 comprises a first piston rod 21, a second piston rod 271 and a third piston rod 281 are respectively provided at both ends of the first piston rod 21, a first plug rod 27 and a second plug rod 28 are respectively provided on the second piston rod 271 and the third piston rod 281, the first piston rod 21 penetrates the second piston rod 271, a first piston pipe 22 is provided on the second piston rod 271, a first piston block 23 is further provided at one end of the second piston rod 271, the first piston rod 21 penetrates the first piston block 23 and a second piston block 24 is further provided, a first feed barrel 25 is provided on the first piston pipe 22 at the upper part of the first piston block 23, a second feed barrel 26 is provided on the first piston pipe 22 at the upper part of the second piston block 24, and a feed pipe 29 is further provided on the first piston pipe 22; The ph balancing mechanism 3 includes a material storage barrel 31, on which a water inlet pipe 33 is also provided, on which a second piston pipe 35 is provided, on which a fourth piston rod 36 is provided, one end of which is fixedly connected to the third piston rod 281, on which a water inlet barrel 34 is also provided, on which a limiting pipe 37 is also provided, and on which a limiting ball 38 is provided.

[0022] Before the reaction starts, nicotinamide and purine nucleoside mixture are put into the first feed barrel 25 as raw materials, and the amount of raw materials entering the reactor 1 is controlled by adjusting the second piston rod 271. Then, enzyme solution is put into the second feed barrel 26 as a catalyst for the reaction, and the amount of catalyst entering the reactor 1 is controlled by adjusting the first piston rod 21. The first plug-in rod 27 is connected to the first piston rod 21 and the second piston rod 271 to control whether the first plug-in rod 27 is connected to the first piston rod 21. The amount of raw materials added and the amount of catalyst added can be controlled separately as needed to maximize the reaction and obtain the product nicotinamide riboside to the maximum extent.

[0023] At the same time, since water is produced in the process of generating nicotinamide riboside, the pH change of the environment affects the activity of the enzyme. Therefore, after the reaction starts, the connection between the fourth piston rod 36 and the first piston rod 21 is adjusted by adjusting the connection between the second plug-in rod 28 and the third piston rod 281 and the first piston rod 21. The amount of alkaline solution added to the water inlet barrel 34 is adjusted by adjusting the first piston rod 21 and the fourth piston rod 36 to enter the water inlet pipe 33 and the storage barrel 31, so that it is convenient to enter the reactor 1 from the storage barrel 31 during the reaction, so as to prevent the reaction from being affected by manual adjustment of the pH value during the reaction, and automatically adjust the pH balance to ensure the normal reaction and efficiently produce nicotinamide riboside.

[0024] At the same time, the motor 16 is turned on to drive the rotating rod 12, which drives the stirring paddle 13 to rotate, and the mixed liquid in the reactor 1 is stirred. At the same time, the stirring paddle 13 squeezes the limiting ball 38 on the inner wall of the reactor 1, so that the alkaline solution in the storage barrel 31 enters the interior of the reactor 1 through the limiting tube 37, neutralizes the water produced by the reaction, adjusts the pH value of the reaction environment, prevents the water produced by the reaction from causing the pH value of the reaction environment to change and affect the activity of the enzyme, affect the efficiency of the reaction, and ensure that the reaction is carried out efficiently.

[0025] In an optional embodiment, the first piston pipe 22 and the feed pipe 29 are hollow in design, and their sizes are compatible with the second piston block 24 and the first piston block 23 .

[0026] It should be noted that the first piston pipe 22 is designed to be hollow, so that the first piston block 23 and the second piston block 24 can move in the first piston pipe 22 to adjust the feed amount.

[0027] In an optional embodiment, the second piston rod 271 and the end of the first piston rod 21 close to the first piston block 23 are both provided with holes, and the size of the holes is adapted to the first plug-in rod 27 .

[0028] It should be noted that when the first connecting rod 27 is detached from the device, moving the first piston rod 21 does not drive the second piston rod 271 to move, and only the second piston block 24 is adjusted. When the first connecting rod 27 is inserted into the device, the first piston rod 21 and the second piston rod 271 are fixed. Moving the first piston rod 21 can drive the second piston rod 271 to move, and at the same time, the first piston block 23 and the second piston block 24 can be adjusted, thereby achieving the purpose of adjusting the feed simultaneously or individually.

[0029] In an optional embodiment, the third piston rod 281 and the end of the first piston rod 21 close to the second piston block 24 are both provided with holes whose sizes are adapted to the second plug-in rod 28 .

[0030] It should be noted that when the second plug-in rod 28 is detached from the device, moving the first piston rod 21 does not drive the fourth piston rod 36 to move. When the second plug-in rod 28 is inserted into the first piston rod 21, the third piston rod 281 is fixed to the first piston rod 21. Moving the first piston rod 21 drives the third piston rod 281 to move, and drives the fourth piston rod 36 to move, thereby adjusting the water inlet volume in the water inlet barrel 34, thereby achieving the purpose of separately adjusting the feed and water inlet, thereby improving the practicability of the device.

[0031] In an optional embodiment, the third piston rod 281 is fixedly connected to the fourth piston rod 36 , and the second piston pipe 35 is hollow in design and has a size that matches the fourth piston rod 36 .

[0032] It should be noted that the movement of the third piston rod 281 drives the fourth piston rod 36 to move in the second piston pipe 35 to adjust the feeding speed of the alkaline solution in the water inlet barrel 34 .

[0033] In an optional embodiment, the reactor 1 is provided with a plurality of holes, the sizes of which are respectively adapted to the discharge plate 15 and the handle 11 .

[0034] It should be noted that the motor 16 is turned on to drive the rotating rod 12 to rotate, drive the stirring paddle 13 to rotate, and stir the reaction. At the same time, the rotation of the stirring paddle 13 drives the scraper 14 to scrape the solution on the inner wall of the reactor 1 to make the reaction more complete.

[0035] In an optional embodiment, a scraper 14 is provided on the stirring paddle 13 , and the scraper 14 is in contact with the inner wall of the reaction kettle 1 .

[0036] It should be noted that after the reaction is completed, the handle 11 is rotated to drive the discharge plate 15 to rotate, and the solution after the reaction is completed is taken out from the reactor 1 to prevent the solution from remaining in the device and facilitate the separation of the generated products.

[0037] In an optional embodiment, the limiting tube 37 is designed to be hollow, and grooves of different sizes are provided inside the limiting tube 37 , and the limiting ball 38 is restricted in the groove by the limiting tube 37 .

[0038] It should be noted that when the stirring paddle 13 rotates, it squeezes the limiting ball 38 and pushes the limiting ball 38 a certain distance, so that the alkaline solution enters the reactor 1 from the storage barrel 31 through the limiting tube 37, maintains the pH balance of the reaction in the reactor 1, and ensures that the reaction proceeds normally and efficiently.

[0039] In an optional embodiment, a support column 32 is provided on the material storage barrel 31, and the material storage barrel 31 and the water inlet pipe 33 are both hollow in design.

[0040] It should be noted that the support column 32 fixedly supports the material storage barrel 31 .

[0041] A method for preparing nicotinamide riboside by a bioenzymatic method comprises the following steps: Step 1: Put nicotinamide and purine nucleoside mixture as raw materials from the first feed barrel 25, and control the amount of raw materials entering the reactor 1 by adjusting the second piston rod 271, and then put enzyme solution as a catalyst for the reaction from the second feed barrel 26, and control the amount of catalyst entering the reactor 1 by adjusting the first piston rod 21, and control whether the first plug-in rod 27 is connected to the first piston rod 21 and the second piston rod 271 by plugging the first plug-in rod 27 with the first piston rod 21; Step 2: After the reactants and catalyst are added to the reactor 1, the connection between the fourth piston rod 36 and the first piston rod 21 is adjusted by adjusting the connection between the second plug-in rod 28 and the third piston rod 281 and the first piston rod 21, and the amount of the alkaline solution added to the water inlet barrel 34 is adjusted by adjusting the first piston rod 21 and the fourth piston rod 36, and enters the water inlet pipe 33 and enters the storage barrel 31; Step three: Turn on the motor 16 to drive the rotating rod 12, which drives the stirring paddle 13 to rotate, and stir the mixed liquid in the reactor 1. At the same time, the stirring paddle 13 squeezes the limiting ball 38 on the inner wall of the reactor 1, so that the alkaline solution in the storage barrel 31 enters the interior of the reactor 1 through the limiting tube 37, neutralizes the water produced by the reaction, and adjusts the pH value of the reaction environment.

[0042] Working principle: Before the reaction starts, nicotinamide and purine nucleoside mixture are put into the first feed barrel 25 as raw materials, and the amount of raw materials entering the reactor 1 is controlled by adjusting the second piston rod 271. Then, enzyme solution is put into the second feed barrel 26 as a catalyst for the reaction, and the amount of catalyst entering the reactor 1 is controlled by adjusting the first piston rod 21. The first plug-in rod 27 is connected to the first piston rod 21 and the second piston rod 271 to control whether the first plug-in rod 27 is connected to the first piston rod 21. The amount of raw materials and the amount of catalyst added can be controlled separately as needed to maximize the reaction and obtain the product nicotinamide riboside to the maximum extent.

[0043] At the same time, since water is produced in the process of generating nicotinamide riboside, the pH change of the environment affects the activity of the enzyme. Therefore, after the reaction starts, the connection between the fourth piston rod 36 and the first piston rod 21 is adjusted by adjusting the connection between the second plug-in rod 28 and the third piston rod 281 and the first piston rod 21. The amount of alkaline solution added to the water inlet barrel 34 is adjusted by adjusting the first piston rod 21 and the fourth piston rod 36 to enter the water inlet pipe 33 and the storage barrel 31, so that it is convenient to enter the reactor 1 from the storage barrel 31 during the reaction, so as to prevent the reaction from being affected by manual adjustment of the pH value during the reaction, and automatically adjust the pH balance to ensure the normal reaction and efficiently produce nicotinamide riboside.

[0044] At the same time, the motor 16 is turned on to drive the rotating rod 12, which drives the stirring paddle 13 to rotate, and the mixed liquid in the reactor 1 is stirred. At the same time, the stirring paddle 13 squeezes the limiting ball 38 on the inner wall of the reactor 1, so that the alkaline solution in the storage barrel 31 enters the interior of the reactor 1 through the limiting tube 37, neutralizes the water produced by the reaction, adjusts the pH value of the reaction environment, prevents the water produced by the reaction from causing the pH value of the reaction environment to change and affect the activity of the enzyme, affect the efficiency of the reaction, and ensure that the reaction is carried out efficiently.

[0045] The first piston pipe 22 is hollow in design, so that the first piston block 23 and the second piston block 24 can move in the first piston pipe 22 to adjust the feed amount. When the first plug-in rod 27 is out of the device, moving the first piston rod 21 does not drive the second piston rod 271 to move, and only the second piston block 24 is adjusted. When the first plug-in rod 27 is inserted into the device, the first piston rod 21 is fixed to the second piston rod 271, and moving the first piston rod 21 can drive the second piston rod 271 to move, and at the same time, the first piston block 23 and the second piston block 24 can be adjusted, so as to achieve the purpose of adjusting the feed simultaneously or separately. When the second plug-in rod 28 is out of the device, moving the first piston rod 21 does not drive the fourth piston rod 36 to move. When the second plug-in rod 28 is inserted into the first piston When the stopper rod 21 is in the middle, the third piston rod 281 is fixed to the first piston rod 21. Moving the first piston rod 21 drives the third piston rod 281 to move, and drives the fourth piston rod 36 to move, so as to adjust the water inlet amount in the water inlet bucket 34, so as to adjust the feed and water inlet respectively, and improve the practicality of the device. After the reaction is completed, the discharge plate 15 is driven to rotate by rotating the handle 11, and the solution after the reaction is completed is taken out from the reactor 1 to prevent the solution from remaining in the device, and at the same time facilitate the separation of the generated products. When the stirring paddle 13 rotates, the limiting ball 38 is squeezed, and the limiting ball 38 is pushed a certain distance, so that the alkaline solution enters the reactor 1 from the storage barrel 31 through the limiting tube 37, so as to maintain the pH balance of the reaction in the reactor 1 and ensure the normal and efficient reaction.

[0046] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A preparation device for preparing nicotinamide riboside using a bioenzymatic method, comprising a reaction kettle (1), characterized in that: The reactor (1) is provided with an adjustment mechanism (2), and the reactor (1) is also provided with a pH balance mechanism (3); The reaction kettle (1) comprises a motor (16), a rotating rod (12) is arranged on the motor (16), a stirring paddle (13) is arranged on the rotating rod (12), and a discharge plate (15) is arranged on the reaction kettle (1), and a handle (11) is arranged on the discharge plate (15); The regulating mechanism (2) comprises a first piston rod (21), a second piston rod (271) and a third piston rod (281) are respectively provided at both ends of the first piston rod (21), a first plug-in rod (27) and a second plug-in rod (28) are respectively provided on the second piston rod (271) and the third piston rod (281), the first piston rod (21) passes through the second piston rod (271), a first piston pipe (22) is provided on the second piston rod (271), a first piston block (23) is further provided at one end of the second piston rod (271), the first piston rod (21) passes through the first piston block (23) and is further provided with a second piston block (24), a first piston pipe (22) is provided on the upper part of the first piston block (23) A feed barrel (25), a second feed barrel (26) is arranged on the first piston pipe (22) at the upper part of the second piston block (24), and a feed pipe (29) is also arranged on the first piston pipe (22); the pH balance mechanism (3) comprises a storage barrel (31), a water inlet pipe (33) is also arranged on the storage barrel (31), a second piston pipe (35) is arranged on the water inlet pipe (33), a fourth piston rod (36) is arranged on the second piston pipe (35), one end of the fourth piston rod (36) is fixedly connected to the third piston rod (281), a water inlet barrel (34) is also arranged on the water inlet pipe (33), a limiting pipe (37) is also arranged on the storage barrel (31), and a limiting ball (38) is arranged on the limiting pipe (37).

2. The device for preparing nicotinamide riboside by using a biological enzyme method according to claim 1, characterized in that: The first piston pipe (22) and the feed pipe (29) are hollow in design, and their sizes are compatible with the second piston block (24) and the first piston block (23).

3. The device for preparing nicotinamide riboside by using a biological enzyme method according to claim 1, characterized in that: The second piston rod (271) and the end of the first piston rod (21) close to the first piston block (23) are both provided with holes, and the size of the holes is adapted to the first plug-in rod (27).

4. The device for preparing nicotinamide riboside by using a biological enzyme method according to claim 1, characterized in that: The third piston rod (281) and the first piston rod (21) are both provided with holes at one end close to the second piston block (24), and the size of the holes is adapted to the second plug-in rod (28).

5. The device for preparing nicotinamide riboside by using a biological enzyme method according to claim 1, characterized in that: The second piston pipe (35) is hollow in design and has a size that matches that of the fourth piston rod (36).

6. The device for preparing nicotinamide riboside by using a biological enzyme method according to claim 5, characterized in that: The reaction kettle (1) is provided with a plurality of holes, the sizes of which are respectively adapted to the discharge plate (15) and the handle (11).

7. The device for preparing nicotinamide riboside by using a biological enzyme method according to claim 1, characterized in that: The stirring paddle (13) is provided with a scraper (14), and the scraper (14) is in contact with the inner wall of the reaction kettle (1).

8. The device for preparing nicotinamide riboside by using a biological enzyme method according to claim 1, characterized in that: The limiting tube (37) is of hollow design, and grooves of different sizes are arranged inside the limiting tube (37), and the limiting ball (38) is restricted in the groove by the limiting tube (37).

9. The device for preparing nicotinamide riboside by using a biological enzyme method according to claim 1, characterized in that: A support column (32) is provided on the material storage barrel (31), and the material storage barrel (31) and the water inlet pipe (33) are both hollow in design.

10. A method for preparing nicotinamide riboside by a biological enzyme method, using the preparation device for preparing nicotinamide riboside by a biological enzyme method as described in any one of 1 to 9 above, characterized in that: The steps include: Step 1: Nicotinamide and purine nucleoside mixed solution are put into the first feed barrel (25) as raw materials, and the amount of raw materials entering the reaction kettle (1) is controlled by adjusting the second piston rod (271); enzyme solution is put into the second feed barrel (26) as a catalyst for the reaction, and the amount of catalyst entering the reaction kettle (1) is controlled by adjusting the first piston rod (21); and the first plug-in rod (27) is connected to the first piston rod (21) and the second piston rod (271) to control whether the first plug-in rod (27) is connected to the first piston rod (21); Step 2: After the reactants and the catalyst are added to the reactor (1), the connection between the fourth piston rod (36) and the first piston rod (21) is adjusted by adjusting the connection between the second plug-in rod (28) and the third piston rod (281) and the first piston rod (21), and the amount of the alkaline solution added to the water inlet barrel (34) is adjusted by adjusting the first piston rod (21) and the fourth piston rod (36), so that the alkaline solution enters the water inlet pipe (33) and enters the storage barrel (31); Step 3: The motor (16) is turned on to drive the rotating rod (12), which drives the stirring paddle (13) to rotate, so that the mixed liquid in the reactor (1) is stirred. At the same time, the stirring paddle (13) squeezes the limiting ball (38) on the inner wall of the reactor (1), so that the alkaline solution in the storage barrel (31) enters the interior of the reactor (1) through the limiting tube (37), neutralizes the water generated by the reaction, and adjusts the pH value of the reaction environment.

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