Broccoli seed aqueous extract partner composition for improving glucoraphanin conversion rate as well as preparation method and application of broccoli seed aqueous extract partner composition

Abstract

The invention relates to the technical field of food, in particular to a broccoli seed aqueous extract partner composition capable of improving the glucoraphanin conversion rate and a preparation method and application thereof. The partner composition comprises a broccoli tender tissue extract and horseradish tree leaves, wherein the broccoli tender tissue extract is broccoli powder or broccoli bud seedling powder. The broccoli seed aqueous extract partner composition is prepared by mixing a broccoli tender tissue extract (broccoli powder or broccoli bud seedling powder) and moringa oleifera leaf powder. The partner composition and the broccoli seed aqueous extract are used together, so that the problems of poor stability of sulforaphane and low bioavailability of glucoraphanin in the prior art are successfully solved, and compared with single use of the broccoli seed aqueous extract, the partner composition disclosed by the invention has the advantages that the conversion rate of glucoraphanin can be increased by 2649-2886%; compared with the combination of the moringa oleifera leaf aqueous extract and the broccoli seed aqueous extract, the conversion rate can be increased by 1817.1%-1854.8%.

Description

Technical Field

[0001] The invention relates to the field of food technology, and in particular to a broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin, and a preparation method and application thereof. Background Art

[0002] Broccoli, also known as green cauliflower, cauliflower, and broccoli, is a plant of the genus Brassica in the family Cruciferae and is a popular vegetable. Glucoraphanin is a natural active product mainly found in broccoli seeds and sprouts, which can be converted into sulforaphane after the action of myrosinase or intestinal microorganisms. Sulforaphane is one of the strongest activators of Nrf2 discovered so far. It can activate Nrf2 to activate multiple cell protection effects such as detoxification, anti-oxidation, and anti-inflammatory. It has extremely strong anti-cancer and anti-cancer effects and is called natural anti-cancer gold. It is the most powerful substance with anti-cancer effect extracted from plants known so far. The water extract of broccoli seeds is rich in glucoraphane, which is a precursor of sulforaphane, and it is sulforaphane that really works in the human body.

[0003] Although sulforaphane has good biological activity, its stability is very poor, and its degradation rate is particularly fast in water and under high temperature conditions. Its application is greatly limited. Therefore, there are very few sulforaphane health products or pharmaceutical products on the market. The existing patent CN202111300863.7 discloses a glucoraphanin and Moringa extract composition lozenge and its application. The Moringa extract is an extract of Moringa leaves, or Moringa stems, or Moringa seeds, which is mixed evenly with maltodextrin and spray dried. The Moringa lozenge includes 60-80 parts by weight of Moringa extract and 5-10 parts by weight of glucoraphanin, as well as sodium carboxymethyl cellulose and / or citric acid and / or menthol and / or sucrose and / or magnesium stearate. After mixing, it is extruded by a punch to form a sheet of Moringa lozenge. The thermal stability of myrosinase in Moringa seed extract is higher than that of broccoli extract, and it still has good stability at 85°C. It can stably enzymatically hydrolyze glucoraphanin in the mouth and digestive tract to produce sulforaphane, providing the human body with highly active sulforaphane. Although single glucoraphanin has good stability, its biological absorption and utilization rate in the human body is very low. It needs to be partially converted into sulforaphane after the action of intestinal microorganisms, and the conversion rate is only 5%-10%. Although the above scheme uses Moringa extract to improve the thermal stability of the enzyme, the enzyme from a single source may be limited in activity or quantity, so its conversion rate for glucoraphanin is still limited. Summary of the invention

[0004] In view of this, the present invention proposes a broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin, and a preparation method and application thereof, so as to solve the problem of low conversion rate of glucoraphanin in broccoli in the human body.

[0005] The technical solution of the present invention is achieved in this way:

[0006] In a first aspect, the present invention provides a broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin, wherein the companion composition comprises a broccoli young tissue extract and Moringa leaves, wherein the broccoli young tissue extract is broccoli powder or broccoli sprout powder.

[0007] On the basis of the above technical solution, preferably, the mass ratio of the broccoli young tender tissue extract to the Moringa leaves is 1:2.4-2.6.

[0008] The broccoli seed water extract companion composition prepared by the present invention mainly provides myrosinase from Moringa leaves, and the young and tender tissues of broccoli contain glucoraphanin and part of myrosinase. When eating broccoli, people chew and other methods, and part of myrosinase is released to hydrolyze glucoraphanin to form sulforaphane, but this state lasts for a short time, and the amount of myrosinase released is very small, so the bioavailability is extremely low. In contrast, the high myrosinase activity of Moringa leaves is utilized in the present invention, and the myrosinase of Moringa leaves and broccoli powder can work synergistically to hydrolyze glucoraphanin with various microorganisms in the stomach and intestines, so that sulforaphane can maintain a high concentration and activity in the intestines, and is absorbed and utilized in the stomach and intestines, thereby improving the bioavailability. The present invention achieves a significant improvement in the conversion rate of glucoraphanin by cleverly combining the young and tender tissue extract of broccoli and Moringa leaves with the water extract of broccoli seeds, and solves the problems of poor stability of sulforaphane and low bioavailability of glucoraphanin in the prior art.

[0009] In a second aspect, the present invention provides a method for preparing a broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin, comprising the following steps:

[0010] S1. Grinding broccoli or broccoli sprouts into powder to obtain broccoli young tissue extract;

[0011] S2, drying the Moringa leaves, grinding the dried Moringa leaves into powder to obtain Moringa leaf powder;

[0012] S3. Weigh the broccoli young tissue extract and Moringa leaf powder in proportion and mix them evenly to obtain a broccoli seed water extract companion composition.

[0013] On the basis of the above technical scheme, preferably, step S1 specifically includes: selecting fresh broccoli or broccoli sprouts and freeze-drying them, wherein drying can effectively remove moisture without destroying enzyme activity; crushing the dried broccoli or broccoli sprouts at a speed of 8000-10000 rpm for 5-8 min to ensure that the tissue structure is fully crushed and the myrosinase is released; passing the crushed material through a 60-80 mesh sieve to obtain a broccoli young tissue extract, wherein crushing and sieving are beneficial to subsequent uniform mixing with other ingredients and improving bioavailability.

[0014] On the basis of the above technical scheme, preferably, in step S1, freeze-drying includes the following steps: pre-freeze the broccoli or broccoli sprouts at -30-35°C for 3 hours, and then place them in a vacuum freeze drying oven, and dry them for 22 hours at a vacuum degree of 6.8-8Pa and a condensation temperature below -50°C.

[0015] On the basis of the above technical scheme, preferably, step S2 specifically includes: selecting fresh Moringa leaves, drying at 30-35° C. for 6-8 hours, so as to remove moisture while retaining the active ingredients; crushing the dried Moringa leaves at a speed of 6000-8000 rpm for 3-5 minutes, and passing the crushed Moringa leaf powder through an 80-100 mesh sieve to obtain Moringa leaf powder, wherein the powder state helps to increase the surface area of ​​the active ingredients in the Moringa leaves and improve its interaction with other ingredients, thereby more effectively playing its role in promoting the conversion of glucoraphanin.

[0016] In a third aspect, the present invention provides an application of a broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin, wherein the companion composition and the broccoli seed water extract are mixed evenly to obtain a powdered product, and the powdered product is applied to functional foods.

[0017] On the basis of the above technical solution, preferably, the content of glucoraphanin in the broccoli seed water extract is 15-20%.

[0018] On the basis of the above technical solution, preferably, the mass ratio of the companion composition to the broccoli seed water extract is 1:1-4.

[0019] On the basis of the above technical scheme, preferably, the preparation method of broccoli seed water extract comprises: selecting fresh broccoli seeds, and crushing the broccoli seeds after drying, thereby increasing the contact area between the broccoli seeds and water and improving the extraction efficiency of glucoraphanin by drying and crushing the broccoli seeds; mixing the crushed broccoli seed powder with water, heating for extraction, filtering the extract, collecting the filtrate, and then concentrating and spray-drying the filtrate to obtain the broccoli seed water extract.

[0020] On the basis of the above technical scheme, preferably, the mass ratio of the crushed broccoli seed powder to water is 1:5-1:10, the heating extraction temperature is 60-70°C, and the extraction time is 2-3h, which can achieve efficient extraction while ensuring the stability of glucoraphanin.

[0021] The invention provides an application of a broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin. The powdered product is mixed with an auxiliary material at a mass ratio of 1:0.5-1.2, and then extruded by a punch to form a sheet product. The sheet product is applied to functional foods.

[0022] On the basis of the above technical solution, preferably, the auxiliary materials include any one or more of fillers, disintegrants, lubricants, binders and flavoring agents.

[0023] On the basis of the above technical scheme, preferably, the filler is selected from one or more of microcrystalline cellulose, lactose, mannitol, and sorbitol; the disintegrant is selected from one or more of cross-linked sodium carboxymethyl cellulose, sodium carboxymethyl starch, and cross-linked polyvinylpyrrolidone; the lubricant is selected from one or two of magnesium stearate and talc; the binder is selected from one or two of povidone K30 and hypromellose; the flavoring agent is selected from one or more of steviol glycosides, sucrose, and xylitol.

[0024] The broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin of the present invention and its preparation method and application have the following beneficial effects compared with the prior art:

[0025] The present invention prepares a broccoli seed water extract companion composition by mixing a broccoli young tissue extract (broccoli powder or broccoli sprout powder) and moringa leaf powder. The companion composition and the broccoli seed water extract are used together to successfully solve the problems of poor stability of sulforaphane and low bioavailability of glucoraphanin in the prior art. Compared with the use of broccoli seed water extract alone, the companion composition of the present invention can increase the conversion rate of glucoraphanin by 2649-2886%, and compared with the combination of only using moringa leaves and broccoli seed water extracts, the conversion rate can be increased by 1817.1%-1854.8%. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0027] Figure 1The present invention is a schematic flow chart of a method for preparing a broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin. DETAILED DESCRIPTION

[0028] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] Example 1

[0030] This embodiment provides a broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin and a preparation method and application thereof, such as Figure 1 As shown, the following steps are included:

[0031] (1) Select fresh broccoli, wash it, place the washed broccoli in an oven at 45° C. and dry it for 10 h, place the dried broccoli in a grinder, grind it at a speed of 9000 rpm for 6 min, and pass the grinded material through an 80-mesh sieve to obtain a broccoli young tissue extract;

[0032] (2) Collect fresh Moringa leaves, clean them and remove impurities, place the cleaned Moringa leaves in an oven at 35° C. and dry them for 7 h, place the dried Moringa leaves in a grinder, grind them at a speed of 7000 rpm for 4 min, and pass the crushed Moringa leaf powder through a 100-mesh sieve to obtain Moringa leaf powder;

[0033] (3) Weigh 10 g of broccoli young tissue extract and 25 g of Moringa leaf powder and mix them to obtain a companion composition, then mix the companion composition and 30 g of broccoli seed water extract evenly to obtain a broccoli seed water extract composition.

[0034] Example 2

[0035] The present embodiment provides a broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin, and a preparation method and application thereof. The preparation method is the same as that of Example 1, except that: in step (3), 10 g of broccoli young tissue extract and 25 g of Moringa leaf powder are weighed and mixed to obtain a companion composition, and then the companion composition and 40 g of broccoli seed water extract are evenly mixed to obtain a broccoli seed water extract composition.

[0036] Example 3

[0037] This embodiment provides a broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin, and a preparation method and application thereof, comprising the following steps:

[0038] (1) Select fresh broccoli, wash it, place the washed broccoli in an oven at 50° C. and dry it for 8 h, place the dried broccoli in a grinder, grind it at a speed of 10,000 rpm for 5 min, and pass the grinded material through an 80-mesh sieve to obtain a broccoli young tissue extract;

[0039] (2) Collect fresh Moringa leaves, clean them and remove impurities, place the cleaned Moringa leaves in an oven at 35° C. and dry them for 6 h, place the dried Moringa leaves in a grinder, grind them at a speed of 8000 rpm for 3 min, and pass the crushed Moringa leaf powder through a 100-mesh sieve to obtain Moringa leaf powder;

[0040] (3) Weigh 10 g of broccoli young tissue extract and 26 g of Moringa leaf powder and mix them to obtain a companion composition, then mix the companion composition and 40 g of broccoli seed water extract evenly to obtain a broccoli seed water extract composition.

[0041] Example 4

[0042] This embodiment provides a broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin, and a preparation method and application thereof. The preparation method is the same as that of Example 1, except that: in step (3), 10 g of broccoli young tissue extract and 25 g of Moringa leaf powder are weighed and mixed to obtain a companion composition, and then the companion composition and 20 g of broccoli seed water extract are evenly mixed to obtain a broccoli seed water extract composition.

[0043] Example 5

[0044] The present embodiment provides a broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin, and a preparation method and application thereof. The preparation method is the same as that of Example 1, except that: in step (3), 10 g of broccoli young tissue extract and 25 g of Moringa leaf powder are weighed and mixed to obtain a companion composition, and then the companion composition and 10 g of broccoli seed water extract are evenly mixed to obtain a broccoli seed water extract composition.

[0045] Example 6

[0046] This embodiment provides a broccoli seed water extract composition for improving the conversion rate of glucoraphanin and a preparation method and application thereof, comprising the following steps:

[0047] (1) Select fresh broccoli, wash it, place the washed broccoli in an oven at 40° C. and dry it for 12 h, place the dried broccoli in a grinder, grind it at a speed of 8000 rpm for 8 min, and pass the grinded material through a 60-mesh sieve to obtain a broccoli young tissue extract;

[0048] (2) Collect fresh Moringa leaves, clean them and remove impurities, place the cleaned Moringa leaves in an oven at 30° C. and dry them for 8 h, place the dried Moringa leaves in a grinder, grind them at a speed of 6000 rpm for 5 min, and pass the crushed Moringa leaf powder through an 80-mesh sieve to obtain Moringa leaf powder;

[0049] (3) Select fresh broccoli seeds, clean and dry them, place the dried broccoli seeds in a grinder, grind them at a speed of 6000 rpm for 5 min, mix the crushed broccoli seed powder with water in a mass ratio of 1:5, stir and extract the mixture at 60° C. for 3 h, filter the extract, collect the filtrate, concentrate the filtrate to 1 / 3 of the original volume, and spray dry to obtain a broccoli seed water extract;

[0050] (4) Weigh 10 g of broccoli young tissue extract, 24 g of Moringa leaf powder and 10 g of broccoli seed water extract, mix them evenly to obtain a broccoli seed water extract composition.

[0051] Example 7

[0052] The present embodiment provides a broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin, and a preparation method and application thereof. The preparation method is the same as that of Example 1, except that: in step (3), 10 g of broccoli young tissue extract and 25 g of Moringa leaf powder are weighed and mixed to obtain a companion composition, and then the companion composition and 3 g of broccoli seed water extract are evenly mixed to obtain a broccoli seed water extract composition.

[0053] Example 8

[0054] The present embodiment provides a broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin, and a preparation method and application thereof. The preparation method is the same as that of Example 1, except that: in step (3), 10 g of broccoli young tissue extract and 25 g of Moringa leaf powder are weighed and mixed to obtain a companion composition, and then the companion composition and 5 g of broccoli seed water extract are evenly mixed to obtain a broccoli seed water extract composition.

[0055] Comparative Example 1

[0056] This comparative example provides a broccoli seed water extract and a preparation method thereof, comprising the following steps:

[0057] Select fresh broccoli seeds, clean and dry them, place the dried broccoli seeds in a grinder, grind them at 7000 rpm for 4 minutes, mix the crushed broccoli seed powder with water in a mass ratio of 1:8, stir and extract the mixture at 65°C for 2.5 hours, filter the extract, collect the filtrate, concentrate the filtrate to 1 / 4 of the original volume, and spray dry to obtain a broccoli seed water extract.

[0058] Comparative Example 2

[0059] This comparative example provides a broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin and a preparation method and application thereof, comprising the following steps:

[0060] (1) Collect fresh Moringa leaves, clean them and remove impurities, place the cleaned Moringa leaves in an oven at 35° C. and dry them for 7 h, place the dried Moringa leaves in a grinder, grind them at a speed of 7000 rpm for 4 min, and pass the crushed Moringa leaf powder through a 100-mesh sieve to obtain Moringa leaf powder;

[0061] (2) Weigh 20 g of Moringa leaf powder and 10 g of broccoli seed water extract, mix them evenly, and obtain a broccoli seed water extract composition.

[0062] Comparative Example 3

[0063] This comparative example provides a broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin and a preparation method and application thereof, comprising the following steps:

[0064] (1) Select fresh broccoli, wash it, place the washed broccoli in an oven at 45° C. and dry it for 10 h, place the dried broccoli in a grinder, grind it at a speed of 9000 rpm for 6 min, and pass the grinded material through an 80-mesh sieve to obtain a broccoli young tissue extract;

[0065] (2) Weigh 20 g of broccoli young tissue extract and 10 g of broccoli seed water extract, mix them evenly, and obtain a broccoli seed water extract composition.

[0066] Performance Testing

[0067] Weigh the broccoli seed water extract composition samples prepared in the example and the comparative example, add 20 times of hydrochloric acid solution with a pH of 1.8, stir evenly, and place in a 37°C constant temperature box for 3 hours. After filtering, take 1 ml of the filtrate and add 8 ml of methanol, shake well, react for 10 minutes, pass through an organic filter membrane, and detect the sulforaphane content. The test results are shown in Table 1.

[0068] Table 1 Glucoraphanin conversion rate

[0069]

[0070]

[0071] In summary, when the mass ratio of broccoli young tissue extract, Moringa leaf and broccoli seed water extract is 1:2.4-2.6:1-4, the conversion rate of glucoraphanin is the highest. Moreover, the broccoli seed water extract companion composition provided by the present invention can significantly improve the efficiency of glucoraphanin conversion into sulforaphane, thereby improving the human bioavailability, which is 2649-2886% higher than that of the simple broccoli seed water extract and 1817.1%-1854.8% higher than the conversion rate of Moringa leaf and broccoli seed water extract.

[0072] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin, characterized in that: The companion composition comprises broccoli young and tender tissue extract and moringa leaves, wherein the broccoli young and tender tissue extract is broccoli powder or broccoli sprout powder.

2. The broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin according to claim 1, characterized in that: The mass ratio of the broccoli young tissue extract to the Moringa leaf is 1:2.4-2.

6.

3. The method for preparing a broccoli seed aqueous extract companion composition for improving the conversion rate of glucoraphanin according to any one of claims 1 to 2, characterized in that: The following steps are involved: S1. Grinding broccoli or broccoli sprouts into powder to obtain broccoli young tissue extract; S2, drying the Moringa leaves, grinding the dried Moringa leaves into powder to obtain Moringa leaf powder; S3. Weigh the broccoli young tissue extract and Moringa leaf powder in proportion, mix them evenly, and obtain a broccoli seed water extract companion composition.

4. The method for preparing a broccoli seed aqueous extract companion composition for improving the conversion rate of glucoraphanin according to claim 3, characterized in that: Step S1 specifically includes: selecting fresh broccoli or broccoli sprouts, freeze-drying, crushing the dried broccoli or broccoli sprouts at a speed of 8000-10000 rpm for 5-8 min, passing the crushed material through a 60-80 mesh sieve, and obtaining a broccoli young tissue extract.

5. The method for preparing a broccoli seed aqueous extract companion composition for improving the conversion rate of glucoraphanin according to claim 3, characterized in that: Step S2 specifically includes: selecting fresh Moringa leaves, drying them at 30-35°C for 6-8h, crushing the dried Moringa leaves at a speed of 6000-8000 rpm for 3-5min, and passing the crushed Moringa leaf powder through an 80-100 mesh sieve to obtain Moringa leaf powder.

6. The use of a broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin according to any one of claims 1 to 2, characterized in that: The companion composition and the broccoli seed water extract are mixed evenly to obtain a powdered product, and the powdered product is used in functional foods.

7. The use of a broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin according to any one of claim 6, characterized in that: The content of glucoraphanin in the broccoli seed water extract is 15-20%.

8. The use of a broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin according to any one of claim 6, characterized in that: The mass ratio of the companion composition to the broccoli seed water extract is 1:1-4.

9. The use of a broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin according to any one of claim 6, characterized in that: The powdered product is mixed with auxiliary materials at a mass ratio of 1:0.5-1.2 and then extruded by a punch to form a sheet product, which is applied to functional foods.

10. The broccoli seed water extract companion composition for improving the conversion rate of glucoraphanin according to claim 9, characterized in that: The auxiliary materials include any one or more of fillers, disintegrants, lubricants, binders and flavoring agents.

Images