China-hemp peptide nutritional protein powder and preparation method thereof

Abstract

The invention belongs to the field of preparation of nutritional protein powder, and particularly relates to China-hemp peptide nutritional protein powder and a preparation method thereof. The method comprises the following steps: carrying out enzymolysis fermentation on protein slurry through a zymophyte solution, carrying out ultrasonication on the prepared slurry after enzymolysis, continuously stirring to react so as to combine the China-hemp peptide with exopolysaccharide generated by microorganisms, and mixing the nutrient enrichment slurry with an extracting solution, so that the nutrient function of the obtained China-hemp peptide-containing nutrient component is remarkably improved; hydroxyl groups in the polyvinyl alcohol and ester groups of sucrose octaacetate form a three-dimensional network structure through interaction, the absorption-promoting solution fully adsorbs the metabolism-promoting solution, and the obtained absorption-promoting metabolism-promoting agent can promote nutrient absorption of body cells; after the stability-maintaining storable mixed solution and the absorption and metabolism promoting agent are mixed, the China-hemp peptide-containing nutritional components are added, and the obtained China-hemp peptide nutritional protein powder is comprehensive in nutrition, high in absorptivity, capable of improving the intestinal function after being eaten, high in stability and not prone to deterioration after being stored.

Description

Technical Field

[0001] The invention belongs to the field of preparation of nutritional protein powder, and specifically refers to hemp peptide nutritional protein powder and a preparation method thereof. Background Art

[0002] Hemp peptides are bioactive peptides extracted from hemp seeds and prepared through processes such as enzymatic hydrolysis. Hemp is an annual herbaceous plant of the genus Cannabis in the family Cannabaceae. Its seeds are rich in protein, amino acids, unsaturated fatty acids and a variety of bioactive ingredients. Hemp peptides are obtained by hydrolyzing the protein in hemp seeds into small molecule peptides. These peptides have physical and chemical properties such as good solubility, thermal stability and low viscosity.

[0003] Hemp peptides are rich in amino acids, including 8 essential amino acids for the human body, and do not contain anti-nutritional factors. They are easy to digest and absorb. They have a high protein content and are an ideal source of nutritional supplements, especially for infants, the elderly, people recovering from illness and the weak. They have significant antioxidant activity, can remove free radicals in the body, and delay aging. The biologically active ingredients in them can enhance the body's immunity and improve resistance. Hemp peptides are rich in arginine and branched-chain amino acids, which help relieve fatigue, improve physical strength, and have physiological functions such as lowering blood pressure and blood sugar.

[0004] At present, the existing nutritional protein powder preparation technology has the following problems: First, the existing protein powder preparation process leads to the destruction of protein and amino acids, reduces their digestibility and utilization, and reduces the efficacy of protein powder; Second, the existing protein powder is easily oxidized and corrupted in a high temperature and high humidity environment, and is contaminated by microorganisms, so it cannot be stored for too long, and the efficacy is unstable during storage; Third, the existing protein powder is for people who are allergic to dairy products or have lactose intolerance. If milk-based protein powder is used, it may cause gastrointestinal discomfort, and long-term consumption may lead to diarrhea. Summary of the invention

[0005] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a hemp peptide nutritional protein powder and a preparation method thereof. In order to solve the problems of the existing nutritional protein powder such as incomplete nutrition, poor absorbability causing gastrointestinal discomfort, unstable quality, and easy deterioration after storage, the present invention uses a hemp peptide-containing nutritional component, an absorption and metabolism promoting agent, and a stabilizing and storable mixed liquid for mixing. The prepared hemp peptide nutritional protein powder is comprehensive in nutrition, highly absorbable, improves intestinal function after consumption, has strong stability, and is not easy to deteriorate after storage.

[0006] In order to achieve the above object, the technical solution adopted by the present invention is as follows: the present invention proposes a hemp peptide nutritional protein powder, and the raw materials for preparing the hemp peptide nutritional protein powder specifically include the following components in parts by weight: It contains 45-55 parts of hemp peptide nutritional components, 23-26 parts of absorption and metabolism promoting agents, 2.1-2.4 parts of sodium stearoyl lactylate, 1.1-1.3 parts of ε-polylysine hydrochloride, and 1.2-1.6 parts of ascorbyl palmitate.

[0007] Preferably, the raw materials for preparing the hemp peptide-containing nutritional components include the following components in parts by weight: 28-32 parts of hemp seeds, 13-17 parts of buckwheat, 11-14 parts of salmon, 6-9 parts of walnut kernels, 14-18 parts of olive oil, 7-12 parts of echinacea, 8-10 parts of dandelion, 6.8-8.2 parts of cardamine, and 1.3-1.6 parts of fermentation agent.

[0008] Preferably, the raw materials for preparing the absorption-promoting and metabolism-promoting agent include the following components in parts by weight: 7-10 parts of black ginger extract, 4-6 parts of linalool, 4.5-6.5 parts of green coffee extract, 5.2-6.7 parts of polyvinyl alcohol, and 4.8-6.9 parts of sucrose octaacetate.

[0009] Preferably, the method for preparing the hemp peptide-containing nutritional component specifically comprises the following steps: S1. Put hemp seeds, buckwheat, salmon, walnut kernels and olive oil into a pulping machine with a power of 1.8-2.4 kW, the pulping temperature is 85-90°C, the pulping time is 35-40 min, the pulping speed is 18000-20000 r / min, and the protein slurry is obtained; S2, putting echinacea, dandelion and cardamine into a pulverizer with a power of 1.5-1.8 kW, the pulverizing temperature is 23-28°C, the pulverizing time is 15-20 min, the pulverizing speed is 3500-4500 r / min, and after pulverization, putting them into an ultrasonic extractor with a power of 1.3-1.6 kW with a 50% ethanol solution, the extraction pressure is 1.5 MPa, the extraction frequency is 25 kHz, the extraction temperature is 40-45°C, the extraction time is 8-13 min, and extraction is performed to obtain an extract; S3, placing the fermentation bacteria and 5% sterile glucose solution in a constant temperature shaking incubator with a power of 2.2-2.5 kW, the constant temperature shaking incubation temperature is 30°C, the constant temperature shaking incubation time is 24-28 h, the constant temperature shaking incubation speed is 210-230r / min, and after the constant temperature shaking incubation, a fermentation bacterial liquid is obtained; S4, placing the fermentation broth prepared by S3 and the protein slurry prepared by S1 into an enzymolysis tank with a power of 2.2-2.6 kW, the enzymolysis speed is 110-140 r / min, the enzymolysis temperature is 33-35°C, the enzymolysis time is 3.6-4.5 h, stirring and enzymolysis to obtain an enzymolysis slurry; S5, putting the enzymatic hydrolysis slurry prepared in S4 into an ultrasonic cell disruptor with a power of 1.2 kW, an ultrasonic crushing frequency of 20 kHz, an ultrasonic crushing temperature of 0°C, and an ultrasonic crushing time of 6 min. After ultrasonic crushing, putting it into a constant temperature stirring tank with a power of 2.8 kW, a constant temperature stirring temperature of 35°C, a constant temperature stirring time of 1.2 h, a constant temperature stirring speed of 500 r / min, stirring for reaction, filtering with a sterile filter cloth, and obtaining a nutrient-fortified slurry; S6. Put the nutritional fortified slurry prepared in S5 and the extract prepared in S2 into a stirrer with a power of 1.2-1.6 kW, stir at a temperature of 23-26°C, stir for 18-25 min, and stir at a speed of 500 r / min. After mixing, obtain the hemp peptide-containing nutritional components.

[0010] Furthermore, in S2, the solid-liquid ratio of the echinacea to the 50% ethanol solution is 1:1.5 g / mL.

[0011] Further, in S3, the solid-liquid ratio of the fermentation agent to the 5% sterile glucose solution is 1:100 g / mL, the fermentation agent is composed of Lactobacillus kvisogenetics, Debaryomyces hansenii, and Corynebacterium glutamicum, and the mixing ratio by weight is 1.2:1.1:1, the Lactobacillus kvisogenetics is purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC1.2831, the Debaryomyces hansenii is purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC2.3948, and the Corynebacterium glutamicum is purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC1.1888.

[0012] Preferably, the method for preparing the absorption-promoting and metabolism-promoting agent comprises the following steps: L1. Place black ginger extract, linalool, green coffee extract and ultrapure water into a stirring tank with a power of 1.5-1.8 kW, stir at a temperature of 26-30°C, stir for 22-26 min, and stir at a speed of 350-550 r / min to dissolve and obtain a metabolic solution; L2, polyvinyl alcohol, sucrose octaacetate, ultrapure water, and ethyl acetate were placed in a constant temperature stirring tank with a power of 2.8 kW, the constant temperature stirring temperature was 35°C, the constant temperature stirring time was 1.2 h, the constant temperature stirring speed was 500 r / min, and the reaction was stirred to obtain an absorption-promoting solution; L3. Put the absorption-promoting solution prepared by L2 into the metabolism-promoting solution prepared by L1, stir at a temperature of 25-30°C, for 20-26 min, at a speed of 300-400 r / min, mix well for sufficient adsorption, and obtain an absorption-promoting and metabolism-promoting agent.

[0013] Furthermore, in L1, the mass fraction of the black ginger extract in ultrapure water is 4.6%.

[0014] Furthermore, in L2, the mass fraction of the polyvinyl alcohol in ultrapure water is 8-12%, and the mass fraction of the sucrose octaacetate in ethyl acetate is 10-13%.

[0015] The present invention also proposes a method for preparing hemp peptide nutritional protein powder, which specifically comprises the following steps: Step 1: Sodium stearoyl lactylate, ε-polylysine hydrochloride, ascorbyl palmitate, and 70% ethanol solution are placed in a stirring tank with a power of 1.5-1.8 kW, the stirring temperature is 28-35°C, the stirring time is 15-20 min, the stirring speed is 400-600 r / min, and the mixture is fully dissolved to obtain a stable and storable mixed solution; Step 2: Add the absorption and metabolism promoting agent into the stabilizing and storage-resistant mixed solution prepared in step 1, stir at a temperature of 30-36°C, stir for 15-20 min, and stir at a speed of 400-500 r / min. After stirring and adsorbing, add the hemp peptide-containing nutritional component, stir at a temperature of 28-33°C, stir for 25-30 min, and stir at a speed of 500 r / min to obtain a mixture; Step 3: Place the mixture prepared in step 2 into a vacuum freeze-nano spray dryer with a power of 2.3-2.8 kW, the vacuum degree is -0.05 MPa, the freeze-nano spray drying temperature is -40°C, the freeze-nano spray drying particle size is 100nm, the freeze-nano spray drying time is 2.5-3 h, and freeze-nano spray drying is performed to obtain hemp peptide nutritional protein powder.

[0016] Preferably, in step one, the mass fraction of the sodium stearoyl lactylate in the 70% ethanol solution is 3-5%.

[0017] The beneficial effects achieved by the present invention are as follows: The present invention adopts hemp seeds, buckwheat, salmon, walnut kernels and olive oil for mixed grinding to obtain a protein slurry containing protein, fat, dietary fiber, vitamins and minerals, carbohydrates and antioxidant components, and echinacea, dandelion and cardamine are crushed and extracted. The obtained extract has immunomodulatory, anti-inflammatory and antioxidant effects, and also has the effects of regulating blood lipids and lowering blood sugar, etc. The fermentation bacterial liquid containing kefir-producing Lactobacillus, Debaryomyces hansen and Corynebacterium glutamicum is used to enzymatically ferment the protein slurry, and the complex components are decomposed into small molecules such as hemp peptides that are easily absorbed by the body, thereby improving its bioavailability, and can also reduce the anti-nutritional factors in the protein slurry, improve the absorption efficiency of nutrients, and enzymatically hydrolyze the protein slurry. After ultrasonically crushing the slurry, the extracellular polysaccharides in the fermented bacterial broth can form a complex with the hemp peptides and fatty acids contained in the slurry after enzymatic hydrolysis, thereby improving the solubility and stability of the nutrients and reducing their degradation and loss during the digestion process. In addition, after combining with the extracellular polysaccharides produced by microorganisms, hemp peptides and fatty acids can also play a synergistic anti-inflammatory effect by regulating the intestinal flora and the immune system. Extracellular polysaccharides, as prebiotics, promote the growth of beneficial bacteria in the intestine and effectively maintain the homeostasis of the gastrointestinal tract. After the nutrient-enriched slurry and the extract are stirred and mixed, the nutritional components containing hemp peptides are rich in nutrition and comprehensive in nutrition, and the nutritional function is significantly improved. Black ginger extract reduces visceral fat and subcutaneous fat by regulating lipid metabolism. The polymethoxylated flavonoids contained in it can accelerate metabolism, especially for the decomposition of abdominal fat. The linden glycosides and green coffee extracts can inhibit the intestinal absorption of fat and carbohydrates, reduce the rapid rise of blood sugar, and thus reduce the possibility of fat synthesis. They can also promote the production of short-chain fatty acids by changing the composition of intestinal flora. These short-chain fatty acids can further regulate fat metabolism. After polyvinyl alcohol and sucrose octaacetate are mixed, the hydroxyl groups in polyvinyl alcohol and the ester groups of sucrose octaacetate form cross-linking points through hydrogen bonds or electrostatic interactions. These cross-linking points connect the polyvinyl alcohol molecular chains together to form a three-dimensional network structure, which fully adsorbs the metabolism-promoting solution with a network structure. The absorption and metabolism promoting agents obtained can promote the absorption of nutrients by body cells, promote the body's metabolism and prevent obesity; sodium stearoyl lactylate, ε-polylysine hydrochloride, and ascorbyl palmitate are dissolved in 70% ethanol solution, and the obtained stable and storable mixture can form a complex with protein, enhance the stability of protein, prevent aggregation and precipitation between proteins, effectively remove free radicals, inhibit the growth of microorganisms, and ensure that the efficacy is maintained without deterioration during storage. After the stable and storable mixture is mixed with the absorption and metabolism promoting agents, the hemp peptide-containing nutritional components are added, and after freeze-nano spray drying, the hemp peptide nutritional protein powder obtained has comprehensive nutrition, strong absorbability, improves intestinal function after consumption, has strong stability, and is not easy to deteriorate after storage. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the present invention or the technical solutions in the prior art, the following will be described in a clear and understandable manner in conjunction with the accompanying drawings. Obviously, the drawings described below are only for the present invention, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0019] Figure 1 This is a graph showing the results of the determination of the antioxidant capacity of the mouse small intestine described in Experimental Example 1 of the present invention; Figure 2 This is the result of the determination of the antioxidant capacity of mouse liver described in Experimental Example 1 of the present invention Figure 3 This is a graph showing the results of the determination of the antioxidant capacity of the mouse small intestine after storage as described in Experimental Example 3 of the present invention; Figure 4 This is a graph showing the results of the determination of the antioxidant capacity of mouse liver after storage as described in Experimental Example 3 of the present invention; Figure 5 This is a picture of the finished product of the hemp peptide nutritional protein powder described in the present invention. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments; based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0021] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein may be applied to the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only and are not intended to limit the content of this application.

[0022] The experimental methods in the following examples are conventional methods unless otherwise specified; the experimental materials and test strains used in the following examples are purchased from commercial channels unless otherwise specified.

[0023] Example 1: This example provides a hemp peptide nutritional protein powder and a preparation method thereof, wherein the hemp peptide nutritional protein powder comprises the following components in parts by weight: Contains 45 parts of hemp peptide nutritional components, 23 parts of absorption and metabolism promoting agents, 2.1 parts of sodium stearoyl lactylate, 1.1 parts of ε-polylysine hydrochloride, and 1.2 parts of ascorbyl palmitate.

[0024] The raw materials for preparing the hemp peptide-containing nutritional components include the following components in parts by weight: 28 parts of hemp seeds, 13 parts of buckwheat, 11 parts of salmon, 6 parts of walnut kernels, 14 parts of olive oil, 7 parts of echinacea, 8 parts of dandelion, 6.8 parts of cardamine, and 1.3 parts of fermentation agent.

[0025] The raw materials for preparing the absorption-promoting and metabolism-promoting agent include the following components in parts by weight: 7 parts of black ginger extract, 4 parts of linalool, 4.5 parts of green coffee extract, 5.2 parts of polyvinyl alcohol, and 4.8 parts of sucrose octaacetate.

[0026] The method for preparing the hemp peptide-containing nutritional component specifically comprises the following steps: S1. Put hemp seeds, buckwheat, salmon, walnut kernels and olive oil into a 1.8 kW refiner at a refining temperature of 85°C, a refining time of 35 min and a refining speed of 18000 r / min to obtain a protein slurry; S2, put echinacea, dandelion and cardamine into a 1.5 kW grinder, the grinding temperature is 23°C, the grinding time is 15 min, the grinding speed is 3500 r / min, and after grinding, put them into an ultrasonic extractor with a power of 1.3 kW and a 50% ethanol solution, the extraction pressure is 1.5 MPa, the extraction frequency is 25 kHz, the extraction temperature is 40°C, the extraction time is 8 min, and extraction is performed to obtain an extract; S3, placing the fermentation bacteria and 5% sterile glucose solution in a constant temperature shaking incubator with a power of 2.2 kW, the constant temperature shaking culture temperature is 30°C, the constant temperature shaking culture time is 24 h, the constant temperature shaking culture speed is 210 r / min, and after the constant temperature shaking culture, a fermentation bacterial liquid is obtained; S4, putting the fermentation liquid prepared by S3 and the protein slurry prepared by S1 into an enzymolysis tank with a power of 2.2 kW, the enzymolysis speed is 110 r / min, the enzymolysis temperature is 33°C, the enzymolysis time is 3.6 h, stirring and enzymolysis to obtain an enzymolysis slurry; S5, putting the enzymatic hydrolysis slurry prepared in S4 into an ultrasonic cell disruptor with a power of 1.2 kW, an ultrasonic crushing frequency of 20 kHz, an ultrasonic crushing temperature of 0°C, and an ultrasonic crushing time of 6 min. After ultrasonic crushing, putting it into a constant temperature stirring tank with a power of 2.8 kW, a constant temperature stirring temperature of 35°C, a constant temperature stirring time of 1.2 h, a constant temperature stirring speed of 500 r / min, stirring for reaction, filtering with a sterile filter cloth, and obtaining a nutrient-fortified slurry; S6. Put the nutritional fortified slurry prepared in S5 and the extract prepared in S2 into a 1.2 kW agitator with a stirring temperature of 23°C, a stirring time of 18 min, and a stirring speed of 500 r / min. After mixing, a nutritional component containing hemp peptides is obtained.

[0027] In S2, the solid-liquid ratio of the echinacea to the 50% ethanol solution is 1:1.5 g / mL.

[0028] In S3, the solid-liquid ratio of the fermentation agent to the 5% sterile glucose solution is 1:100 g / mL, and the fermentation agent is composed of Lactobacillus kefir, Debaryomyces hansen, and Corynebacterium glutamicum, and the mixing ratio by weight is 1.2:1.1:1. The Lactobacillus kefir was purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC1.2831, the Debaryomyces hansen was purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC2.3948, and the Corynebacterium glutamicum was purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC1.1888.

[0029] The preparation method of the absorption-promoting and metabolism-promoting agent specifically comprises the following steps: L1. Place black ginger extract, linalool, green coffee extract and ultrapure water into a stirring tank with a power of 1.5 kW, stir at a temperature of 26°C, for 22 min, at a stirring speed of 350 r / min, and stir to dissolve to obtain a metabolic solution; L2, polyvinyl alcohol, sucrose octaacetate, ultrapure water, and ethyl acetate were placed in a constant temperature stirring tank with a power of 2.8 kW, the constant temperature stirring temperature was 35°C, the constant temperature stirring time was 1.2 h, the constant temperature stirring speed was 500 r / min, and the reaction was stirred to obtain an absorption-promoting solution; L3. Put the absorption-promoting solution prepared by L2 into the metabolism-promoting solution prepared by L1, stir at 25°C for 20 min, and stir at 300 r / min. Mix well for sufficient adsorption to obtain an absorption-promoting and metabolism-promoting agent.

[0030] In L1, the mass fraction of the black ginger extract in ultrapure water is 4.6%.

[0031] In L2, the mass fraction of the polyvinyl alcohol in ultrapure water is 8%, and the mass fraction of the sucrose octaacetate in ethyl acetate is 10%.

[0032] This embodiment also provides a method for preparing hemp peptide nutritional protein powder, which specifically includes the following steps: Step 1: Sodium stearoyl lactylate, ε-polylysine hydrochloride, ascorbyl palmitate, and 70% ethanol solution are placed in a stirring tank with a power of 1.5 kW, the stirring temperature is 28°C, the stirring time is 15 min, the stirring speed is 400 r / min, and they are fully dissolved to obtain a stable and storable mixed solution; Step 2: Add the absorption-promoting and metabolism-promoting agent into the stabilizing and storage-resistant mixed solution prepared in step 1, stir at a temperature of 30°C, stir for 15 min, and stir at a speed of 400 r / min. After stirring and adsorbing, add the hemp peptide-containing nutritional component, stir at a temperature of 28°C, stir for 25 min, and stir at a speed of 500 r / min to obtain a mixture; Step 3: Place the mixture prepared in step 2 into a vacuum freeze-nano spray dryer with a power of 2.3 kW, the vacuum degree is -0.05 MPa, the freeze-nano spray drying temperature is -40°C, the freeze-nano spray drying particle size is 100 nm, the freeze-nano spray drying time is 2.5 h, and freeze-nano spray drying is performed to obtain hemp peptide nutritional protein powder.

[0033] In step 1, the mass fraction of the sodium stearoyl lactylate in the 70% ethanol solution is 3%.

[0034] Example 2: This example provides a hemp peptide nutritional protein powder and a preparation method thereof, wherein the hemp peptide nutritional protein powder comprises the following components in parts by weight: Contains 50 parts of hemp peptide nutritional components, 25 parts of absorption and metabolism promoting agents, 2.3 parts of sodium stearoyl lactylate, 1.2 parts of ε-polylysine hydrochloride, and 1.4 parts of ascorbyl palmitate.

[0035] The raw materials for preparing the hemp peptide-containing nutritional components include the following components in parts by weight: 30 parts of hemp seeds, 15 parts of buckwheat, 12 parts of salmon, 7 parts of walnut kernels, 16 parts of olive oil, 9 parts of echinacea, 9 parts of dandelion, 7.5 parts of cardamine, and 1.4 parts of fermentation bacteria.

[0036] The raw materials for preparing the absorption-promoting and metabolism-promoting agent include the following components in parts by weight: 8 parts of black ginger extract, 5 parts of linalool, 5.5 parts of green coffee extract, 6 parts of polyvinyl alcohol, and 5.7 parts of octaacetate sucrose.

[0037] The method for preparing the hemp peptide-containing nutritional component specifically comprises the following steps: S1. Put hemp seeds, buckwheat, salmon, walnut kernels and olive oil into a 2.2 kW refiner at a refining temperature of 88°C, a refining time of 36 min and a refining speed of 19000 r / min to obtain a protein slurry; S2, put echinacea, dandelion and cardamine into a 1.6 kW grinder, the grinding temperature is 25°C, the grinding time is 18 min, the grinding speed is 4000 r / min, and after grinding, put them into an ultrasonic extractor with a power of 1.5 kW and a 50% ethanol solution, the extraction pressure is 1.5 MPa, the extraction frequency is 25 kHz, the extraction temperature is 43°C, the extraction time is 10 min, and extract to obtain an extract; S3, placing the fermentation bacteria and 5% sterile glucose solution in a constant temperature shaking incubator with a power of 2.3 kW, the constant temperature shaking culture temperature is 30°C, the constant temperature shaking culture time is 26 h, the constant temperature shaking culture speed is 220 r / min, and after the constant temperature shaking culture, a fermentation solution is obtained; S4, putting the fermentation broth prepared by S3 and the protein slurry prepared by S1 into an enzymolysis tank with a power of 2.5 kW, the enzymolysis speed is 130 r / min, the enzymolysis temperature is 34°C, the enzymolysis time is 4.2 h, stirring and enzymolysis to obtain an enzymolysis slurry; S5, putting the enzymatic hydrolysis slurry prepared in S4 into an ultrasonic cell disruptor with a power of 1.2 kW, an ultrasonic crushing frequency of 20 kHz, an ultrasonic crushing temperature of 0°C, and an ultrasonic crushing time of 6 min. After ultrasonic crushing, putting it into a constant temperature stirring tank with a power of 2.8 kW, a constant temperature stirring temperature of 35°C, a constant temperature stirring time of 1.2 h, a constant temperature stirring speed of 500 r / min, stirring for reaction, filtering with a sterile filter cloth, and obtaining a nutrient-fortified slurry; S6. Put the nutritional fortified slurry prepared in S5 and the extract prepared in S2 into a 1.3 kW agitator with a stirring temperature of 25°C, a stirring time of 22 min, and a stirring speed of 500 r / min. After mixing, a nutritional component containing hemp peptides is obtained.

[0038] In S2, the solid-liquid ratio of the echinacea to the 50% ethanol solution is 1:1.5 g / mL.

[0039] In S3, the solid-liquid ratio of the fermentation agent to the 5% sterile glucose solution is 1:100 g / mL, and the fermentation agent is composed of Lactobacillus kefir, Debaryomyces hansen, and Corynebacterium glutamicum, and the mixing ratio by weight is 1.2:1.1:1. The Lactobacillus kefir was purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC1.2831, the Debaryomyces hansen was purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC2.3948, and the Corynebacterium glutamicum was purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC1.1888.

[0040] The preparation method of the absorption-promoting and metabolism-promoting agent specifically comprises the following steps: L1. Place black ginger extract, linalool, green coffee extract and ultrapure water into a stirring tank with a power of 1.7 kW, stir at a temperature of 28°C, stir for 25 min, and stir at a speed of 400 r / min to dissolve and obtain a metabolic solution; L2, polyvinyl alcohol, sucrose octaacetate, ultrapure water, and ethyl acetate were placed in a constant temperature stirring tank with a power of 2.8 kW, the constant temperature stirring temperature was 35°C, the constant temperature stirring time was 1.2 h, the constant temperature stirring speed was 500 r / min, and the reaction was stirred to obtain an absorption-promoting solution; L3. Put the absorption-promoting solution prepared by L2 into the metabolism-promoting solution prepared by L1, stir at 28°C for 23 min, and stir at 350 r / min. Mix well for sufficient adsorption to obtain an absorption-promoting and metabolism-promoting agent.

[0041] In L1, the mass fraction of the black ginger extract in ultrapure water is 4.6%.

[0042] In L2, the mass fraction of the polyvinyl alcohol in ultrapure water is 10%, and the mass fraction of the sucrose octaacetate in ethyl acetate is 11%.

[0043] This embodiment also provides a method for preparing hemp peptide nutritional protein powder, which specifically includes the following steps: Step 1: Sodium stearoyl lactylate, ε-polylysine hydrochloride, ascorbyl palmitate, and 70% ethanol solution are placed in a stirring tank with a power of 1.6 kW, the stirring temperature is 32°C, the stirring time is 17 min, the stirring speed is 500 r / min, and they are fully dissolved to obtain a stable and storable mixed solution; Step 2: Add the absorption and metabolism promoting agent into the stabilizing and storage-resistant mixed solution prepared in step 1, stir at a temperature of 32°C, stir for 16 min, and stir at a speed of 450 r / min. After stirring and adsorbing, add the hemp peptide-containing nutritional component, stir at a temperature of 31°C, stir for 28 min, and stir at a speed of 500 r / min to obtain a mixture; Step 3: Place the mixture prepared in step 2 into a vacuum freeze-nano spray dryer with a power of 2.6 kW, the vacuum degree is -0.05 MPa, the freeze-nano spray drying temperature is -40°C, the freeze-nano spray drying particle size is 100 nm, the freeze-nano spray drying time is 2.8 h, and freeze-nano spray drying is performed to obtain hemp peptide nutritional protein powder.

[0044] In step 1, the mass fraction of the sodium stearoyl lactylate in the 70% ethanol solution is 4%.

[0045] Example 3: This example provides a hemp peptide nutritional protein powder and a preparation method thereof, wherein the hemp peptide nutritional protein powder comprises the following components in parts by weight: Contains 55 parts of hemp peptide nutritional components, 26 parts of absorption and metabolism promoting agents, 2.4 parts of sodium stearoyl lactylate, 1.3 parts of ε-polylysine hydrochloride, and 1.6 parts of ascorbyl palmitate.

[0046] The raw materials for preparing the hemp peptide-containing nutritional components include the following components in parts by weight: 32 parts of hemp seeds, 17 parts of buckwheat, 14 parts of salmon, 9 parts of walnut kernels, 18 parts of olive oil, 12 parts of echinacea, 10 parts of dandelion, 8.2 parts of cardamine, and 1.6 parts of fermentation bacteria.

[0047] The raw materials for preparing the absorption-promoting and metabolism-promoting agent include the following components in parts by weight: 10 parts of black ginger extract, 6 parts of linalool, 6.5 parts of green coffee extract, 6.7 parts of polyvinyl alcohol, and 6.9 parts of octaacetate sucrose.

[0048] The method for preparing the hemp peptide-containing nutritional component specifically comprises the following steps: S1. Put hemp seeds, buckwheat, salmon, walnut kernels and olive oil into a 2.4 kW refiner at a refining temperature of 90°C, a refining time of 40 min and a refining speed of 20,000 r / min to obtain a protein slurry; S2, put echinacea, dandelion and cardamine into a pulverizer with a power of 1.8 kW, the pulverizing temperature is 28°C, the pulverizing time is 20 min, the pulverizing speed is 4500 r / min, and after pulverization, put them into an ultrasonic extractor with a power of 1.6 kW and a 50% ethanol solution, the extraction pressure is 1.5 MPa, the extraction frequency is 25 kHz, the extraction temperature is 45°C, the extraction time is 13 min, and extraction is performed to obtain an extract; S3, placing the fermentation bacteria and 5% sterile glucose solution in a constant temperature shaking incubator with a power of 2.5 kW, the constant temperature shaking culture temperature is 30°C, the constant temperature shaking culture time is 28 h, the constant temperature shaking culture speed is 230 r / min, and after the constant temperature shaking culture, a fermentation solution is obtained; S4, putting the fermentation broth prepared by S3 and the protein slurry prepared by S1 into an enzymolysis tank with a power of 2.6 kW, the enzymolysis speed is 140 r / min, the enzymolysis temperature is 35°C, the enzymolysis time is 4.5 h, stirring and enzymolysis to obtain an enzymolysis slurry; S5, putting the enzymatic hydrolysis slurry prepared in S4 into an ultrasonic cell disruptor with a power of 1.2 kW, an ultrasonic crushing frequency of 20 kHz, an ultrasonic crushing temperature of 0°C, and an ultrasonic crushing time of 6 min. After ultrasonic crushing, putting it into a constant temperature stirring tank with a power of 2.8 kW, a constant temperature stirring temperature of 35°C, a constant temperature stirring time of 1.2 h, a constant temperature stirring speed of 500 r / min, stirring for reaction, filtering with a sterile filter cloth, and obtaining a nutrient-fortified slurry; S6. Put the nutritional fortified slurry prepared in S5 and the extract prepared in S2 into a 1.6 kW agitator with a stirring temperature of 26°C, a stirring time of 25 min, and a stirring speed of 500 r / min. After mixing, a nutritional component containing hemp peptides is obtained.

[0049] In S2, the solid-liquid ratio of the echinacea to the 50% ethanol solution is 1:1.5 g / mL.

[0050] In S3, the solid-liquid ratio of the fermentation agent to the 5% sterile glucose solution is 1:100 g / mL, and the fermentation agent is composed of Lactobacillus kefir, Debaryomyces hansen, and Corynebacterium glutamicum, and the mixing ratio by weight is 1.2:1.1:1. The Lactobacillus kefir was purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC1.2831, the Debaryomyces hansen was purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC2.3948, and the Corynebacterium glutamicum was purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC1.1888.

[0051] The preparation method of the absorption-promoting and metabolism-promoting agent specifically comprises the following steps: L1. Place black ginger extract, linalool, green coffee extract and ultrapure water into a stirring tank with a power of 1.8 kW, stir at a temperature of 30°C, for 26 min, at a stirring speed of 550 r / min, and stir to dissolve to obtain a metabolic solution; L2, polyvinyl alcohol, sucrose octaacetate, ultrapure water, and ethyl acetate were placed in a constant temperature stirring tank with a power of 2.8 kW, the constant temperature stirring temperature was 35°C, the constant temperature stirring time was 1.2 h, the constant temperature stirring speed was 500 r / min, and the reaction was stirred to obtain an absorption-promoting solution; L3. Put the absorption-promoting solution prepared by L2 into the metabolism-promoting solution prepared by L1, stir at 30°C for 26 min and at 400 r / min, mix well for sufficient adsorption to obtain an absorption-promoting and metabolism-promoting agent.

[0052] In L1, the mass fraction of the black ginger extract in ultrapure water is 4.6%.

[0053] In L2, the mass fraction of the polyvinyl alcohol in ultrapure water is 12%, and the mass fraction of the sucrose octaacetate in ethyl acetate is 13%.

[0054] This embodiment also provides a method for preparing hemp peptide nutritional protein powder, which specifically includes the following steps: Step 1: Sodium stearoyl lactylate, ε-polylysine hydrochloride, ascorbyl palmitate, and 70% ethanol solution are placed in a stirring tank with a power of 1.8 kW, the stirring temperature is 35°C, the stirring time is 20 min, the stirring speed is 600 r / min, and they are fully dissolved to obtain a stable and storable mixed solution; Step 2: Add the absorption and metabolism promoting agent into the stabilizing and storage-resistant mixed solution prepared in step 1, stir at a temperature of 36°C, stir for 20 min, and stir at a speed of 500 r / min. After stirring and adsorbing, add the hemp peptide-containing nutritional component, stir at a temperature of 33°C, stir for 30 min, and stir at a speed of 500 r / min to obtain a mixture; Step 3: Place the mixture prepared in step 2 into a vacuum freeze-nano spray dryer with a power of 2.8 kW, the vacuum degree is -0.05 MPa, the freeze-nano spray drying temperature is -40°C, the freeze-nano spray drying particle size is 100 nm, the freeze-nano spray drying time is 3 h, and freeze-nano spray drying is performed to obtain hemp peptide nutritional protein powder.

[0055] In step 1, the mass fraction of the sodium stearoyl lactylate in the 70% ethanol solution is 5%.

[0056] Comparative Example 1: This comparative example provides a nutritional protein powder containing plant ingredients and a preparation method thereof. The only difference from Example 1 is that the prepared nutritional component containing hemp peptides is not fermented by fermentation bacteria liquid, and the remaining components, component contents, and method steps are the same as Example 1.

[0057] Comparative Example 2: This comparative example provides a nutritional protein powder containing plant ingredients and a preparation method thereof. The only difference from Example 1 is that the prepared absorption-promoting and metabolism-promoting agent does not contain an absorption-promoting solution, and the remaining components, component contents, and method steps are the same as those in Example 1.

[0058] Experimental Example 1: Efficacy determination test.

[0059] The test steps for determining the efficacy of the hemp peptide nutritional protein powder prepared in Examples 1-3 of the present invention are as follows: (1) 42 Kunming mice weighing 25 g were selected and randomly divided into 6 groups, with 7 mice in each group. The mice in Example 1-3 groups were fed with 3.5 g of the hemp peptide nutritional protein powder prepared in Example 1-3 every day for 30 consecutive days, the mice in the comparative example 1-2 groups were fed with 3.5 g of the nutritional protein powder containing plant ingredients prepared in comparative example 1-2 every day for 30 consecutive days, and the mice in the control group were fed with 3.5 g of the control group whey protein powder (purchased from Xiamen Yangzhen Health Food Co., Ltd.) every day for 30 consecutive days; (2) After 30 days of consumption, the mice were killed by cervical dislocation. After dissection, 10 cm of small intestine and 10 g of liver tissue were removed from each Kunming mouse. The contents of total superoxide dismutase (T-SOD), glutathione (GSH), and glutathione peroxidase (GSH-Px) in the tissues were measured using a glutathione detection kit (Shanghai Xinyu, catalog number: XYEA294Ge), a glutathione peroxidase assay kit (AAT Bioquest, catalog number: AAT-B11560), and a total superoxide dismutase detection kit (PERFEMIKER, catalog number: PM13417).

[0060] Result analysis: Figure 1 This is a graph showing the results of the determination of the antioxidant capacity of the mouse small intestine described in Experimental Example 1 of the present invention. Figure 2The results of the determination of the antioxidant capacity of the mouse liver described in Experimental Example 1 of the present invention are shown in the figure. As shown in the figure, the mice in Example 1-3 groups respectively consumed 3.5 g of the hemp peptide nutritional protein powder prepared in Example 1-3 every day. After continuous consumption for 30 days, the total superoxide dismutase content, glutathione, and glutathione peroxidase content in the small intestine of the mice in Example 3 group reached 186.33 U / mg.prot, 28.65 U / mg.prot, and 97.09 U / mg.prot, respectively. The total superoxide dismutase content, glutathione, and glutathione peroxidase content in the liver of the mice in Example 3 group reached 167.33 U / mg.prot, 27.35 U / mg.prot, and 116.46 U / mg.prot, respectively. The mice in the comparative example 1-2 groups respectively consumed 3.5 g of the nutritional protein powder containing plant ingredients prepared in comparative example 1-2 every day for 30 consecutive days. d later, the total superoxide dismutase content, glutathione and glutathione peroxidase content in the small intestine of the mice in the comparison example 1 group reached 155.07 U / mg.prot, 17.64 U / mg.prot and 78.87 U / mg.prot, respectively; the total superoxide dismutase content, glutathione and glutathione peroxidase content in the liver of the mice in the comparison example 1 group reached 136.06 U / mg.prot, 17.69 U / mg.prot and 96.03 U / mg.prot, respectively; the total superoxide dismutase content, glutathione and glutathione peroxidase content in the small intestine of the mice in the comparison example 2 group reached 159.22 U / mg.prot, 20.49 U / mg.prot and 88.80 U / mg.prot, respectively; the total superoxide dismutase content, glutathione and glutathione peroxidase content in the liver of the mice in the comparison example 2 group reached 142.36 U / mg.prot, respectively. The mice in the control group consumed 3.5 g of whey protein powder in the control group every day. After continuous consumption for 30 d, the total superoxide dismutase content, glutathione and glutathione peroxidase content in the small intestine of the mice in the control group reached 139.94 U / mg.prot, 16.13 U / mg.prot and 75.73 U / mg.prot, respectively. The total superoxide dismutase content, glutathione and glutathione peroxidase content in the liver of the mice in the control group reached 113.68 U / mg.prot, 15.57 U / mg.prot and 90.14 U / mg.prot, respectively. This shows that the hemp peptide nutritional protein powder prepared by the present invention has comprehensive nutrition, strong absorbability and significant efficacy after consumption.

[0061] Experimental Example 2: Test for measuring the effect on the intestine.

[0062] The test steps for determining the intestinal effects of the hemp peptide nutritional protein powder prepared in Examples 1-3 of the present invention are as follows: (1) 42 Kunming mice weighing 25 g were selected and randomly divided into 6 groups, with 7 mice in each group. The mice in Example 1-3 groups were fed with 3.5 g of the hemp peptide nutritional protein powder prepared in Example 1-3 every day for 30 consecutive days, the mice in the comparative example 1-2 groups were fed with 3.5 g of the nutritional protein powder containing plant ingredients prepared in comparative example 1-2 every day for 30 consecutive days, and the mice in the control group were fed with 3.5 g of the control group whey protein powder (purchased from Xiamen Yangzhen Health Food Co., Ltd.) every day for 30 consecutive days; (2) After consuming the food for 30 days, the mice were fasted for 12 hours, and the defecation latency and feces particle count of the mice in Example 1-3 groups, Comparative Example 1-2 groups, and the control group were observed.

[0063] Table 1. Mouse defecation status

[0064] Result analysis: As shown in Table 1, after long-term feeding of the hemp peptide nutritional protein powder prepared in Examples 1-3, the defecation latency and defecation quantity of the mice in the Example 1-3 groups were at normal levels, while after long-term feeding of the control group whey protein powder, the defecation latency of the mice in the control group was significantly shortened, the number of fecal particles was higher than the normal level, and diarrhea occurred, indicating that the hemp peptide nutritional protein powder prepared by the present invention improves intestinal function after consumption and does not cause diarrhea after long-term use.

[0065] Experimental Example 3: Stability determination test.

[0066] The stability test steps of the hemp peptide nutritional protein powder prepared in Examples 1-3 of the present invention are as follows: (1) After storing the hemp peptide nutritional protein powder prepared in Example 1-3, the nutritional protein powder containing plant ingredients prepared in Comparative Example 1-2, and the whey protein powder of the control group (purchased from Xiamen Yangzhen Health Food Co., Ltd.) at a temperature of 28° C. and a humidity of 60% for 2 months, the hemp peptide nutritional protein powder prepared in Example 1-3 after storage, the nutritional protein powder containing plant ingredients prepared in Comparative Example 1-2 after storage, and the whey protein powder of the control group after storage were obtained; (2) 42 Kunming mice weighing 25 g were selected and randomly divided into 6 groups, with 7 mice in each group. The mice in Example 1-3 groups were gavaged daily for 30 consecutive days. The mice in the comparative example 1-2 groups were gavaged daily for 3.5 g of the nutritional protein powder containing plant ingredients prepared in comparative example 1-2 after storage for 30 consecutive days. The mice in the control group were gavaged daily for 3.5 g of the whey protein powder of the control group after storage for 30 consecutive days. (3) After 30 days of consumption, the mice were killed by cervical dislocation. After dissection, 10 cm of intestinal tissue and 10 g of liver tissue were removed from each Kunming mouse. The contents of total superoxide dismutase (T-SOD), glutathione (GSH), and glutathione peroxidase (GSH-Px) in the tissues were measured using a glutathione detection kit (Shanghai Xinyu, catalog number: XYEA294Ge), a glutathione peroxidase assay kit (AAT Bioquest, catalog number: AAT-B11560), and a total superoxide dismutase detection kit (PERFEMIKER, catalog number: PM13417). (4) At a temperature of 28°C, 100 g of the stored hemp peptide nutritional protein powder prepared in Examples 1-3 were weighed, added to 500 mL of water, and stirred for 30 min to form a mixture of Example 1-3. 100 g of the stored plant ingredient nutritional protein powder prepared in Comparative Example 1-2 was weighed, added to 500 mL of water, and stirred for 30 min to form a mixture of Comparative Example 1-2. 100 g of the stored control group whey protein powder (purchased from Xiamen Yangzhen Health Food Co., Ltd.) was weighed, added to 500 mL of water, and stirred for 30 min to form a control group mixture. Then, the mixtures of Example 1-3, Comparative Example 1-2, and the control group were left to stand for 48 h to observe whether the mixtures had oil floating or stratification. The test results are shown in Table 2.

[0067] Table 2. Stability test results of protein powder

[0068] Result analysis: Figure 3 This is a graph showing the results of the determination of the antioxidant capacity of the small intestine of mice after storage as described in Experimental Example 3 of the present invention. Figure 4 This is a graph showing the results of the determination of the antioxidant capacity of mouse liver after storage as described in Experimental Example 3 of the present invention. Figure 5This is a finished product diagram of the hemp peptide nutritional protein powder of the present invention. As shown in the figure, the mice in Example 1-3 groups respectively consumed 3.5 g of the hemp peptide nutritional protein powder prepared in Example 1-3 after storage every day. After continuous consumption for 30 days, the total superoxide dismutase content, glutathione, and glutathione peroxidase content in the small intestine of the mice in Example 3 group reached 181.28 U / mg.prot, 26.96 U / mg.prot, and 93.36 U / mg.prot, respectively. The total superoxide dismutase content, glutathione, and glutathione peroxidase content in the liver of the mice in Example 3 group reached 164.67 U / mg.prot, 25.17 U / mg.prot, and 113.38 U / mg.prot, respectively. The mice in the comparative example 1-2 groups respectively consumed 3.5 g of the nutritional protein powder containing plant ingredients prepared in the comparative example 1-2 after storage every day for 30 consecutive days. d later, the total superoxide dismutase content, glutathione and glutathione peroxidase content in the small intestine of the mice in the comparison example 1 group reached 143.48 U / mg.prot, 11.89 U / mg.prot and 69.92 U / mg.prot, respectively; the total superoxide dismutase content, glutathione and glutathione peroxidase content in the liver of the mice in the comparison example 1 group reached 129.01 U / mg.prot, 11.57 U / mg.prot and 89.75 U / mg.prot, respectively; the total superoxide dismutase content, glutathione and glutathione peroxidase content in the small intestine of the mice in the comparison example 2 group reached 147.89 U / mg.prot, 15.57 U / mg.prot and 79.36 U / mg.prot, respectively; the total superoxide dismutase content, glutathione and glutathione peroxidase content in the liver of the mice in the comparison example 2 group reached 134.89 U / mg.prot, respectively The control group mice consumed 3.5 g of whey protein powder of the control group after storage every day. After continuous consumption for 30 days, the total superoxide dismutase content, glutathione and glutathione peroxidase content in the small intestine of the control group mice reached 97.77 U / mg.prot, 7.54 U / mg.prot and 58.53 U / mg.prot, respectively. The total superoxide dismutase content, glutathione and glutathione peroxidase content in the liver of the control group mice reached 97.62 U / mg.prot, 8.06 U / mg.prot and 71.54 U / mg.prot, respectively. It can be obtained from Table 2 that the hemp peptide nutritional protein powder prepared in Examples 1-3 was dissolved and stirred after storage, and there was no floating oil or stratification phenomenon, which shows that the hemp peptide nutritional protein powder prepared by the present invention has strong stability, is not easy to deteriorate after storage, and can play a long-term and stable role.

[0069] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0070] The present invention and its implementation methods are described above, which is not restrictive. The drawings are only one of the implementation methods of the present invention, and the actual application is not limited thereto. In short, if ordinary technicians in the field are inspired by it and design methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention, they should all fall within the protection scope of the present invention.

Claims

1. A hemp peptide nutritional protein powder, characterized in that: The hemp peptide nutritional protein powder comprises the following components in parts by weight: Contains 45-55 parts of hemp peptide nutritional components, 23-26 parts of absorption and metabolism promoting agents, 2.1-2.4 parts of sodium stearoyl lactylate, 1.1-1.3 parts of ε-polylysine hydrochloride, and 1.2-1.6 parts of ascorbyl palmitate; The raw materials for preparing the hemp peptide-containing nutritional component include the following components in parts by weight: 28-32 parts of hemp seeds, 13-17 parts of buckwheat, 11-14 parts of salmon, 6-9 parts of walnut kernels, 14-18 parts of olive oil, 7-12 parts of echinacea, 8-10 parts of dandelion, 6.8-8.2 parts of cardamine, and 1.3-1.6 parts of fermentation agent; The raw materials for preparing the absorption and metabolism promoting agent include the following components in parts by weight: 7-10 parts of black ginger extract, 4-6 parts of linalool, 4.5-6.5 parts of green coffee extract, 5.2-6.7 parts of polyvinyl alcohol, and 4.8-6.9 parts of octaacetate sucrose.

2. The hemp peptide nutritional protein powder according to claim 1, characterized in that: The method for preparing the hemp peptide-containing nutritional component specifically comprises the following steps: S1, mixing hemp seeds, buckwheat, salmon, walnut kernels and olive oil, and grinding to obtain a protein slurry; S2, crushing Echinacea, dandelion and Cardamine, and extracting with 50% ethanol solution to obtain an extract; S3, inoculating the fermentation bacteria into a 5% sterile glucose solution, and performing shaking culture to obtain a fermentation solution; S4, mixing and stirring the fermentation broth prepared in S3 and the protein slurry prepared in S1 for enzymolysis to obtain a slurry after enzymolysis; S5, subjecting the enzymatically hydrolyzed slurry prepared in S4 to ultrasonic crushing, continuing to stir for reaction, and filtering using a sterile filter cloth to obtain a nutritionally fortified slurry; S6. Stir and mix the nutritional fortified slurry prepared in S5 and the extract prepared in S2 to obtain a nutritional component containing hemp peptides.

3. The hemp peptide nutritional protein powder according to claim 2, characterized in that: In S3, the fermentation bacteria agent is composed of Lactobacillus kumiss, Debaryomyces hansenii, and Corynebacterium glutamicum, and the mixing ratio by weight is 1.2:1.1:

1.

4. The hemp peptide nutritional protein powder according to claim 3, characterized in that: The preparation method of the absorption-promoting and metabolism-promoting agent specifically comprises the following steps: L1. Stir and dissolve black ginger extract, linalool, green coffee extract and ultrapure water to obtain a metabolism-promoting solution; L2, polyvinyl alcohol, sucrose octaacetate, ultrapure water and ethyl acetate are stirred for reaction to obtain an absorption-promoting solution; L3. Place the absorption-promoting solution prepared in L2 into the metabolism-promoting solution prepared in L1, mix and fully adsorb to obtain an absorption-promoting and metabolism-promoting agent.

5. A method for preparing the hemp peptide nutritional protein powder according to any one of claims 1 to 4, characterized in that: The specific steps include: Step 1: Stir and dissolve sodium stearoyl lactylate, ε-polylysine hydrochloride, ascorbyl palmitate, and 70% ethanol solution to obtain a stable and storable mixed solution; Step 2: Add the absorption and metabolism promoting agent into the stabilizing and storage-resistant mixed solution prepared in step 1, stir and adsorb, then add the hemp peptide-containing nutritional component, mix well and obtain a mixture; Step 3: subject the mixture prepared in step 2 to vacuum freeze nano spray drying to obtain hemp peptide nutritional protein powder.

Images