Probiotic composite product for relieving pressure and preparation method thereof

Abstract

The invention relates to a probiotic composite product capable of relieving pressure and a preparation method of the probiotic composite product. The probiotic composite product comprises the following probiotic components: lactobacillus paracasei Lpc-37, lactobacillus plantarum N13 and bifidobacterium lactis BLa80. The combination of the three probiotics has a synergistic effect, can regulate intestinal flora, relieve inflammation symptoms, reduce the perception of the body on pressure, and play a role in relieving the pressure.

Description

Technical Field

[0001] The present application relates to the field of food, and in particular to a stress-relieving probiotic compound product and a preparation method thereof. Background Art

[0002] With the accelerated pace of life, work and life pressure, mood swings, and irregular diet and rest have become the norm for modern people, and various health problems have seriously interfered with our lives. Among them, sleep disorders plague people of different ages and are also a precursor to many chronic diseases. Sleep disorders refer to abnormalities in sleep quality and quantity, as well as a series of clinical symptoms that occur during sleep. Long-term sleep disorders can affect personal physiological functions and mental health, not only reducing the quality of life, but also inducing mental illnesses and cardiovascular and cerebrovascular diseases.

[0003] The products currently on the market that regulate sleep disorders include functional supplements (melatonin, theanine) and Chinese medicinal ingredients / plant extracts (saffron extract, Chinese jujube kernel and sleep tea). The mechanism of action of functional supplements (melatonin, theanine) is to regulate the endocrine and nervous systems by supplementing endogenous hormones or neurotransmitters related to sleep or brain nerves, soothing the mood and relaxing the body and mind, thereby improving sleep quality. However, long-term consumption of these products can cause abnormal hormone metabolism regulation, endocrine disorders and other adverse physical reactions. Chinese medicinal ingredients / plant extracts (saffron extract, Chinese jujube kernel and sleep tea), the mechanism of action of these products is that the natural ingredients in them can calm the mind, thereby relaxing the body and mind and improving sleep. However, long-term improper consumption of saffron extract can cause endocrine disorders, especially having a greater impact on women's physiology; Chinese jujube kernel is not suitable for some people with allergies and underlying diseases; sleep tea contains caffeine that can stimulate the excitatory nerves, drinking more tea will increase the number of times you get up at night, and herbal tea will stimulate the stomach and intestines.

[0004] A large number of studies have shown that sleep disorders can be effectively alleviated through emotional regulation, stress relief and improvement of the sleep process. However, there is currently no product on the market that can improve sleep by relieving stress. Summary of the invention

[0005] In view of the above problems, the present application provides a probiotic compound product suitable for use in all age groups and for relieving stress. The probiotic product has the effect of relieving inflammatory symptoms of the body and reducing the body's perceived stress.

[0006] The invention provides a probiotic compound product for relieving stress. The probiotic compound product contains Lactobacillus paracasei Lpc-37, Lactobacillus plantarum N13 and Bifidobacterium lactis BLa80.

[0007] Different from the prior art, the above technical solution specifically adds Lactobacillus paracasei Lpc-37 to the formula. Studies have found that the strain can reduce the perception of stress and anxiety and maintain a relaxed mental state. Existing experimental studies have pointed out that when individuals are injected with inflammatory markers, such as CRP protein and interferon (IFN-α), their incidence of depression is higher than that of the control group that is not injected with inflammatory markers. Inflammation increases the stress of the body, so many people feel a lack of pleasure, and the anti-inflammatory level in patients with depression is usually not high. The Lactobacillus plantarum N13 and Bifidobacterium lactis BLa80 added to the formula can regulate intestinal flora, adhere to intestinal cells, reduce the expression of inflammatory factors, relieve inflammatory symptoms, and thus relieve the body's stress; at the same time, it can form a synergistic effect with Lactobacillus paracasei Lpc-37, which can regulate the intestinal microecology, relieve inflammatory symptoms, and promote the relief of the body's stress.

[0008] Furthermore, the probiotic compound product also includes the following probiotic components: Lactobacillus rhamnosus and Pediococcus acidilactici.

[0009] Furthermore, the probiotic composite product includes the following components in parts by mass: 12-20 parts by mass of resistant dextrin; 15-24 parts by mass of xylo-oligosaccharide; 12-20 parts by mass of stachyose; 6-10 parts by mass of fructo-oligosaccharide; 0.1-0.4 parts of Lactobacillus plantarum N13; 0.5-1.1 parts of Lactobacillus rhamnosus LRa05; 0.08-0.12 parts of Bifidobacterium lactis BLa80; 0.05-0.2 parts of Pediococcus acidilactici CCFM7902; and 0.1-10 parts of Lactobacillus paracasei Lpc-37.

[0010] The probiotic mixed powder includes four prebiotics: resistant dextrin, oligoxylose, oligofructose, and stachyose. The above prebiotics provide conditions for the growth of lactic acid bacteria, promote the growth and proliferation of probiotics, and make the effect of probiotic powder better. This technical solution uses the above probiotics and prebiotics in combination, which can effectively relieve stress and enhance the product's stress-relieving effect. At the same time, it can promote the proliferation of beneficial intestinal bacteria, inhibit the growth and reproduction of harmful bacteria, and selectively regulate the intestinal flora, making it more suitable for a wider range of people.

[0011] Furthermore, the probiotic mixed powder also includes: 8-14 parts by weight of erythritol and 2-23 parts by weight of cranberry fruit powder. The addition of erythritol and cranberry fruit powder plays a role in adjusting the taste of the product.

[0012] Furthermore, the content of live bacteria in the probiotic composite product is 1.0×10 11 CFU / g and above.

[0013] At present, the bacterial activity of probiotic products in the domestic market is relatively low, most of which are below 2.0×10 10CFU / g-6.0×10 10 The probiotics Lactobacillus paracasei Lpc-37, Lactobacillus plantarum N13 and Bifidobacterium lactis BLa80 of the present invention are all preferred strains, and their bacterial activity is much higher than other products on the market.

[0014] The present invention also discloses a preparation method of the probiotic composite product, which comprises the following steps:

[0015] Mixing: after uniformly mixing Lactobacillus paracasei Lpc-37, Lactobacillus plantarum N13, Bifidobacterium lactis BLa80, Lactobacillus rhamnosus LRa05 and Pediococcus acidilactici CCFM7902 in proportion, continue to add resistant dextrin, xylo-oligosaccharide, stachyose, fructo-oligosaccharide, erythritol and cranberry fruit powder in proportion, mix uniformly to obtain probiotic mixed powder;

[0016] Sealing: canning and sealing the probiotic mixed powder to obtain a probiotic composite product.

[0017] Furthermore, the mixing and sealing steps are performed in a nitrogen atmosphere.

[0018] The inventor found in the study that the outside air has a certain influence on the activity of probiotics during the production process, which will cause the number of live bacteria of probiotics to drop greatly during the shelf life due to problems such as process and packaging. Therefore, the technical solution adopts a vacuum nitrogen filling process to isolate light and air to maintain the stability of the product and the activity of probiotics. In the temporary storage tank for raw materials, a vacuum sealing method is adopted to isolate the raw materials from the external environment; when loading, nitrogen is filled into the silo to place the raw materials in a nitrogen environment; during the canning process, nitrogen is continuously filled into the package, and a film is applied to seal the probiotic powder so that the probiotic powder is in a nitrogen package. By using vacuum nitrogen filling technology, each component is placed in a nitrogen environment throughout the production process, avoiding the problem of product instability and reduced probiotic activity caused by contact with the outside air. In addition, filling with nitrogen can help control the oxygen and moisture indicators in the inner packaging of the product, better maintain the activity of the product, and slow down the decline in the activity of probiotics during the shelf life.

[0019] Furthermore, in the sealing step, the sealed can is made of food-grade polyethylene.

[0020] The food-grade polyethylene packaging shell is used to effectively block external stimuli such as light and heat, thereby better preserving the activity of probiotics.

[0021] The above-mentioned records related to the invention content are only an overview of the technical solution of the present application. In order to enable ordinary technicians in the field to more clearly understand the technical solution of the present application, and then implement it according to the written contents of the specification, and to make the above-mentioned purpose and other purposes, features and advantages of the present application easier to understand, the specific implementation methods of the present application are explained below. DETAILED DESCRIPTION

[0022] In order to explain the technical content, structural features, achieved objectives and effects of the technical solution in detail, the following is a detailed description in conjunction with specific embodiments.

[0023] In order to explain in detail the possible application scenarios, technical principles, specific schemes that can be implemented, and the purposes and effects that can be achieved, the following is a detailed description in conjunction with the specific embodiments listed. The embodiments described herein are only used to more clearly illustrate the technical solutions of the present application, and are therefore only used as examples, and cannot be used to limit the scope of protection of the present application.

[0024] Reference to "embodiment" herein means that the specific features, structures or characteristics described in conjunction with the embodiment may be included in at least one embodiment of the present application. The term "embodiment" appearing in various places in the specification does not necessarily refer to the same embodiment, nor does it particularly limit its independence or association with other embodiments. In principle, in the present application, as long as there is no technical contradiction or conflict, the various technical features mentioned in the embodiments can be combined in any way to form a corresponding implementable technical solution.

[0025] Unless otherwise defined, the technical terms used in this document have the same meanings as those generally understood by those skilled in the art to which this application belongs; the use of relevant terms in this document is only for describing specific embodiments and is not intended to limit this application.

[0026] In the description of this application, the term "and / or" is an expression used to describe the logical relationship between objects, indicating that three relationships may exist, for example, A and / or B, which means: A exists, B exists, and A and B exist at the same time. In addition, the character " / " in this article generally indicates that the objects before and after are in an "or" logical relationship.

[0027] In the present application, terms such as “first” and “second” are merely used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship of quantity, priority or sequence between these entities or operations.

[0028] Without further limitations, in this application, the words "include", "comprises", "has" or other similar expressions used in the sentences are intended to cover non-exclusive inclusion. These expressions do not exclude the presence of additional elements in the process, method or product including the elements, so that the process, method or product including a series of elements may include not only those limited elements, but also other elements not explicitly listed, or also include elements inherent to such process, method or product.

[0029] Similar to the understanding in the Examination Guidelines, in this application, expressions such as "greater than", "less than", "exceed" and the like are understood to exclude the number itself; expressions such as "above", "below", "within" and the like are understood to include the number itself. In addition, in the description of the embodiments of this application, "multiple" means more than two (including two), and similar expressions related to "multiple" are also understood in this way, such as "multiple groups", "multiple times", etc., unless otherwise clearly and specifically limited.

[0030] Lactobacillus paracasei: Lactobacillus paracasei is a Gram-positive heterofermentative lactic acid bacterium that can be isolated from the human intestine, oral cavity, silage and various dairy products. It is a facultative anaerobic, non-motile, non-spore-forming bacillus or long rod, which appears singly or in pairs; the width of the bacteria is 2.0-4.0μm and the length is 0.8-1.0μm. Lactobacillus paracasei grows best at 10-37℃, does not grow above 40℃, and can only survive for 40s at 72℃.

[0031] Lactobacillus plantarum: The optimal growth temperature of Lactobacillus plantarum (L. plantarum) is 30-35℃. It is anaerobic or facultative anaerobic. The strain is straight or curved rod-shaped, single, sometimes in pairs or chains. The optimal pH is about 6.5. It is a homofermentative lactic acid bacteria. As a probiotic flora of the human gastrointestinal tract, Lactobacillus plantarum has multiple functions such as maintaining the balance of intestinal flora, improving the body's immunity and promoting the absorption of nutrients.

[0032] Bifidobacterium lactis: Bifidobacterium lactis refers to animal bifidobacterium, which is one of the dominant bacteria in the intestines of humans and many mammals. This bacterium is an important physiological bacterium in the intestines of humans and animals. It participates in a series of physiological processes such as immunity, nutrition, digestion and protection, and plays an important role. Animal bifidobacterium does not form spores, is non-motile, and is an anaerobic. The optimal growth temperature is 30-41°C; the optimal fermentation temperature is 35-40°C; the lowest growth temperature is 25-28°C; the highest growth temperature is 43-45°C; the initial growth pH is 6.7-7.0. It does not grow in an environment below pH4.5-5.0 or above pH8.0-8.5. Bifidobacterium lactis is mainly distributed in the human intestine, and is also present in large quantities in the digestive tract of some warm-blooded animals such as pigs, dogs, mice and bees.

[0033] Example 1 A probiotic composite product

[0034] Mixing: 0.08 mass parts of Bifidobacterium lactis BLa80, 0.05 mass parts of Pediococcus acidilactici CCFM7902, 0.1 mass parts of Lactobacillus paracasei Lpc-37, 0.1 mass parts of Lactobacillus plantarum N13, and 0.5 mass parts of Lactobacillus rhamnosus LRa05 are added to the mixing bin, mixed evenly, and then 12 mass parts of resistant dextrin, 15 mass parts of xylo-oligosaccharides, 12 mass parts of stachyose, 6 mass parts of oligofructose, 8 mass parts of erythritol, and 2 mass parts of cranberry fruit powder are added to the mixing bin, mixed evenly, and a probiotic mixed powder is obtained. The mixing process is carried out in a nitrogen environment.

[0035] Sealing: The probiotic mixed powder is canned and sealed in a food-grade polyethylene packaging can in a nitrogen atmosphere to obtain a probiotic composite product.

[0036] The content of live bacteria in the probiotic composite product is 1.0×10 11 CFU / g and above.

[0037] Example 2 A probiotic composite product

[0038] Mixing: 1.1 parts by mass of Lactobacillus rhamnosus LRa05, 0.2 parts by mass of Pediococcus acidilactici CCFM7902, 0.12 parts by mass of Bifidobacterium lactis BLa80, 0.4 parts by mass of Lactobacillus plantarum N13, and 10 parts by mass of Lactobacillus paracasei Lpc-37 are added to a mixing bin, mixed evenly, and then: 20 parts by mass of resistant dextrin, 24 parts by mass of xylo-oligosaccharide, 20 parts by mass of stachyose, 10 parts by mass of fructo-oligosaccharide, 14 parts by mass of erythritol, and 23 parts by mass of cranberry fruit powder are added to obtain a probiotic mixed powder. The mixing process is carried out in a nitrogen environment.

[0039] Sealing: The probiotic mixed powder is canned and sealed in a food-grade polyethylene packaging can in a nitrogen atmosphere to obtain a probiotic composite product.

[0040] The content of live bacteria in the probiotic composite product is 1.0×10 11 CFU / g and above.

[0041] Example 3 A probiotic composite product

[0042] Mixing: 0.1 parts by mass of Pediococcus acidilactici CCFM7902, 1 part by mass of Lactobacillus rhamnosus LRa05, 0.1 parts by mass of Bifidobacterium lactis BLa80, 8 parts by mass of Lactobacillus paracasei Lpc-37, and 0.3 parts by mass of Lactobacillus plantarum N13 are added to a mixing bin, mixed evenly, and then: 15 parts by mass of resistant dextrin; 20 parts by mass of xylo-oligosaccharide; 15 parts by mass of stachyose; 8 parts by mass of oligofructose; 10 parts by mass of erythritol, and 20 parts by mass of cranberry fruit powder are added to obtain a probiotic mixed powder. The mixing process is carried out in a nitrogen environment.

[0043] Sealing: The probiotic mixed powder is canned and sealed in a food-grade polyethylene packaging can in a nitrogen atmosphere to obtain a probiotic composite product.

[0044] The content of live bacteria in the probiotic composite product is 1.0×10 11 CFU / g and above.

[0045] Example 4 A probiotic composite product

[0046] Mixing: 0.3 parts by mass of Lactobacillus plantarum N13, 1 part by mass of Lactobacillus rhamnosus LRa05, 0.1 parts by mass of Bifidobacterium lactis BLa80, 0.1 parts by mass of Pediococcus acidilactici CCFM7902, and 6 parts by mass of Lactobacillus paracasei Lpc-37 are added to a mixing bin, mixed evenly, and then: 15 parts by mass of resistant dextrin; 18 parts by mass of xylo-oligosaccharide; 16 parts by mass of stachyose; 8 parts by mass of oligofructose, 10 parts by mass of erythritol, and 20 parts by mass of cranberry fruit powder are added to obtain a probiotic mixed powder. The mixing process is carried out in a nitrogen environment.

[0047] Sealing: The probiotic mixed powder is canned and sealed in a food-grade polyethylene packaging can in a nitrogen atmosphere to obtain a probiotic composite product.

[0048] The content of live bacteria in the probiotic composite product is 1.0×10 11 CFU / g and above.

[0049] Example 5 A probiotic composite product

[0050] Mixing: 0.9 mass parts of Lactobacillus rhamnosus LRa05, 0.15 mass parts of Pediococcus acidilactici CCFM7902, 0.1 mass parts of Bifidobacterium lactis BLa80, 0.3 mass parts of Lactobacillus plantarum N13, and 0.8 mass parts of Lactobacillus paracasei Lpc-37 are added to a mixing bin, and after uniform mixing, 16 mass parts of resistant dextrin, 22 mass parts of xylo-oligosaccharides, 17 mass parts of stachyose, 8 mass parts of oligofructose, 8 mass parts of erythritol, and 5 mass parts of cranberry fruit powder are added to obtain a probiotic mixed powder. The mixing process is carried out in a nitrogen environment.

[0051] Sealing: The probiotic mixed powder is placed in an ammonia atmosphere and sealed in a food-grade polyethylene packaging can to obtain a probiotic composite product.

[0052] The content of live bacteria in the probiotic composite product is 1.0×10 11 CFU / g and above.

[0053] In this technical solution, Lactobacillus paracasei Lpc-37 is specially added. Studies have found that this strain can reduce the perception of stress and anxiety and maintain a relaxed mental state. When individuals are injected with inflammatory markers, such as CRP protein and interferon (IFN-α), their incidence of depression is higher than that of the control group that is not injected with inflammatory markers. Inflammation increases the stress of the body, so many people feel a lack of pleasure, and the anti-inflammatory level in patients with depression is usually not high. The Lactobacillus plantarum N13 and Bifidobacterium lactis BLa80 added to the formula can regulate intestinal flora, adhere to intestinal cells, reduce the expression of inflammatory factors, relieve inflammatory symptoms, and thus relieve the body's stress; at the same time, it can form a synergistic effect with Lactobacillus paracasei Lpc-37, which can regulate the intestinal microecology, relieve inflammatory symptoms, and promote the relief of the body's stress. .

[0054] Finally, it should be noted that although the above embodiments have been described in the text of the specification of this application, this does not limit the scope of patent protection of this application. All technical solutions generated by replacing or modifying equivalent structures or equivalent processes based on the essential concept of this application using the contents recorded in the text of the specification of this application, as well as directly or indirectly implementing the technical solutions of the above embodiments in other related technical fields, are included in the scope of patent protection of this application.

Claims

1. A probiotic compound product for relieving stress, characterized in that: The probiotic compound product contains Lactobacillus paracasei Lpc-37, Lactobacillus plantarum N13 and Bifidobacterium lactis BLa80.

2. The probiotic composite product according to claim 1, characterized in that: The probiotic compound product also includes the following probiotic components: Lactobacillus rhamnosus LRa05 and Pediococcus acidilactici CCFM7902.

3. The probiotic composite product according to claim 1, characterized in that: The probiotic composite product comprises the following components in parts by weight: 12-20 parts by weight of resistant dextrin; 15-24 parts by weight of xylo-oligosaccharide; 12-20 parts by weight of stachyose; 6-10 parts by weight of fructo-oligosaccharide; 0.5-1.1 parts by weight of Lactobacillus rhamnosus LRa05; 0.05-0.2 parts by weight of Pediococcus acidilactici CCFM7902; 0.08-0.12 parts by weight of Bifidobacterium lactis BLa80; and 0.1-0.4 parts by weight of Lactobacillus plantarum N13. Lactobacillus paracasei Lpc-370.1-10 parts by mass.

4. The probiotic composite product according to claim 3, characterized in that: The probiotic mixed powder also includes: 8-14 parts by weight of erythritol and 2-23 parts by weight of cranberry fruit powder.

5. The probiotic composite product according to any one of claims 1 to 4, characterized in that: The content of live bacteria in the probiotic compound product is 1.0×10 11 CFU / g and above.

6. The method for preparing the probiotic composite product according to claim 4, characterized in that: The preparation method comprises the following steps Mixing: after uniformly mixing Lactobacillus paracasei Lpc-37, Lactobacillus plantarum N13, Bifidobacterium lactis BLa80, Lactobacillus rhamnosus LRa05, and Pediococcus acidilactici CCFM7902 according to a certain proportion, continue to add resistant dextrin, xylo-oligosaccharide, stachyose, fructo-oligosaccharide, erythritol, and cranberry fruit powder according to a certain proportion, and uniformly mix to obtain a probiotic mixed powder; Sealing: canning and sealing the probiotic mixed powder to obtain a probiotic composite product.

7. The preparation method according to claim 6, characterized in that: The mixing and sealing steps are carried out in a nitrogen atmosphere.

8. The preparation method according to claim 6, characterized in that: In the sealing step, the sealed can is made of food-grade polyethylene.