Sodium hyaluronate and tremella polysaccharide composition HAmelt with beautifying effect and preparation method of sodium hyaluronate and tremella polysaccharide composition HAmelt

Abstract

The invention discloses a sodium hyaluronate and tremella polysaccharide composition HAmelt with a beautifying effect and a preparation method thereof, and belongs to the technical field of beautifying and skincare. The preparation method comprises the following steps: preparing a dispersing agent, preparing a cosurfactant and preparing a mixed solution; the preparation method comprises the following steps: preparing a dispersing agent, mixing citric acid and deionized water, stirring at room temperature, adjusting the pH value to 3.5-4, adding corn starch, continuously stirring, standing, carrying out spray drying to obtain mixed powder, grinding the mixed powder, irradiating with an ultraviolet lamp at 70-80 DEG C, mixing with calcium gluconate, carrying out ball milling at room temperature, and drying to obtain the dispersing agent. Grinding the mixed powder to obtain a dispersing agent; the sodium hyaluronate and tremella polysaccharide composition HAmelt prepared by the invention has the effects of moisturizing, resisting oxidation and promoting skin repair, and is uniform in particle size distribution, high in dissolving speed in water and good in storage resistance.

Description

Technical Field

[0001] The invention relates to the technical field of beauty and skin care, and in particular to a sodium hyaluronate and tremella polysaccharide composition HAmella with beauty effects and a preparation method thereof. Background Art

[0002] As people's demand for skin care and beauty continues to increase, the development of skin care products with multiple functions has become an important direction of the current cosmetics industry. Among the many functional ingredients, sodium hyaluronate, Tremella polysaccharide, and grape seed oil have attracted much attention due to their excellent moisturizing, anti-oxidation, and skin repair effects.

[0003] Sodium hyaluronate is a polysaccharide naturally present in the skin. It has a strong water-retaining capacity and can effectively improve dry skin and increase skin elasticity. Tremella polysaccharide is a polysaccharide extracted from Tremella fuciformis. It has moisturizing, anti-oxidation and skin repair effects. Grape seed oil is a plant oil extracted from grape seeds. It is rich in antioxidants, vitamins and essential fatty acids. In the field of beauty, grape seed oil is well-known for its excellent moisturizing, anti-oxidation and skin repair effects. The combined use of sodium hyaluronate, Tremella polysaccharide and grape seed oil can achieve multiple beauty effects.

[0004] However, when sodium hyaluronate, tremella polysaccharide and grape seed oil are mixed, a mixed aqueous solution of sodium hyaluronate and tremella polysaccharide is generally mixed with grape seed oil. Since sodium hyaluronate and tremella polysaccharide are easily soluble in water, while grape seed oil is not easily soluble in water, there is a problem of uneven mixing during mixing. Moreover, grape seed oil contains a large amount of unsaturated fatty acids. After long-term storage, grape seed oil is easily oxidized, resulting in a decrease in the moisturizing, anti-oxidation and skin repair effects of grape seed oil.

[0005] To address the above problems, the most commonly used method is to prepare an emulsion by mixing a mixed aqueous solution of sodium hyaluronate and tremella polysaccharide with grape seed oil, that is, mixing the mixed aqueous solution of sodium hyaluronate and tremella polysaccharide with grape seed oil and an emulsifier to prepare an emulsion. However, the prepared emulsion has a large particle size, uneven particle size distribution, and poor stability, which affects the use effect of the emulsion.

[0006] Microemulsion is a kind of uniform, low viscosity, thermodynamically stable transparent or translucent dispersion system spontaneously formed by oil phase, aqueous phase, surfactant and co-surfactant in appropriate proportion, wherein grape seed oil is used as oil phase, and mixed aqueous solution of sodium hyaluronate and Tremella polysaccharide is used as aqueous phase, under the action of surfactant and co-surfactant, stable microemulsion can be formed. Compared with emulsion, microemulsion has small particle size, uniform particle size distribution and high stability. Microemulsion is prepared by mixing mixed aqueous solution of sodium hyaluronate and Tremella polysaccharide with grape seed oil, so as to obtain a composition with strong stability.

[0007] At present, microemulsions made from a mixed aqueous solution of sodium hyaluronate and Tremella polysaccharide mixed with grape seed oil are mainly used in skin care. Since the skin has limited absorption capacity for skin care products and a slow absorption rate, the effect is not obvious. Oral administration uses the digestive tract for absorption, which has a stronger absorption capacity than the skin and a faster absorption rate, resulting in more obvious effects. Therefore, more and more people tend to take beauty products orally.

[0008] When a microemulsion prepared by mixing a mixed aqueous solution of sodium hyaluronate and Tremella polysaccharide with grape seed oil is used as an oral product, the following problems exist: first, a large amount of surfactants and co-surfactants are required in the microemulsion, and excessive amounts of surfactants and co-surfactants may irritate the digestive system; second, the volume of the microemulsion is large and the transportation cost is high.

[0009] In order to solve the above problems, the most commonly used method is to reduce the dosage of surfactant and co-surfactant, and spray-dry the microemulsion to obtain a powdered product. When in use, the powdered product is dissolved in water and taken orally. However, the obtained powdered product has the following disadvantages: first, reducing the dosage of surfactant and co-surfactant will result in uneven particle size distribution of the prepared powdered product; second, reducing the dosage of surfactant and co-surfactant will affect the dissolution rate of the prepared powdered product in water; third, due to the good hydrophilicity of sodium hyaluronate and Tremella polysaccharide, the prepared powdered product is easy to absorb moisture and has poor storage resistance. Summary of the invention

[0010] In view of the shortcomings of the prior art, the present invention provides a sodium hyaluronate and tremella polysaccharide composition HAmella with cosmetic effects and a preparation method thereof. The prepared sodium hyaluronate and tremella polysaccharide composition HAmella has the effects of moisturizing, anti-oxidation, and promoting skin repair, has a uniform particle size distribution, dissolves quickly in water, and has good storage resistance.

[0011] In order to solve the above technical problems, the technical solution adopted by the present invention is as follows: A method for preparing a sodium hyaluronate and tremella polysaccharide composition HAmella with cosmetic effects, comprising: preparing a dispersant, preparing a co-surfactant, and preparing a mixed solution; The dispersant is prepared by mixing citric acid and deionized water, stirring at a stirring speed of 100-200 rpm for 30-40 minutes at room temperature, adjusting the pH to 3.5-4 with a sodium hydroxide aqueous solution, adding corn starch, continuing stirring for 2-3 hours, standing for 15-16 hours, spray drying, controlling the air inlet temperature to 160-200° C. and the air outlet temperature to 60-90° C., obtaining a mixed powder, grinding the mixed powder to a particle size of less than 150 meshes, and then irradiating the mixed powder with an ultraviolet lamp with a wavelength of 320 nm at 70-80° C. for 2-2.5 hours, mixing the mixed powder with calcium gluconate, and ball milling at room temperature at a ball-to-water ratio of 5-6:1:0.4-0.5 and a rotation speed of 300-400 rpm for 40-50 minutes, drying the mixed powder at 120-140° C. to obtain a mixed powder, and grinding the mixed powder to a particle size of less than 100 meshes to obtain a dispersant; In the preparation of the dispersant, the dosage ratio of citric acid, deionized water, corn starch and calcium gluconate is 50-55g:600-700mL:50-60g:104-110g; The mass concentration of the sodium hydroxide aqueous solution is 30%; The preparation of the co-surfactant comprises mixing gum arabic, sodium hyaluronate and deionized water, stirring at 50-60° C. and at a stirring speed of 100-200 rpm for 1.5-2 hours, spray drying, controlling the air inlet temperature to 160-200° C. and the air outlet temperature to 60-90° C. to obtain mixed sodium hyaluronate, grinding the mixed sodium hyaluronate to a particle size of less than 100 mesh, and then irradiating with an ultraviolet lamp with a wavelength of 320 nm at 70-80° C. for 2-2.5 hours to obtain a co-surfactant; In the preparation of the cosurfactant, the dosage ratio of gum arabic, sodium hyaluronate and deionized water is 20-22 g: 14-17 g: 900-1000 mL; The molecular weight of the sodium hyaluronate is 100-400 kDa; The mixed solution is prepared by mixing sodium hyaluronate, Tremella polysaccharide and deionized water, ultrasonically oscillating at a frequency of 20-25kHz for 30-40min at room temperature, adding sucrose fatty acid ester and co-surfactant, and continuing ultrasonic oscillation for 30-40min to obtain a mixed water phase; grape seed oil and a dispersant are mixed, ultrasonically oscillating at a frequency of 20-25kHz for 20-30min at room temperature to obtain an oil phase; after mixing all the mixed water phase and all the oil phases, high shearing at a speed of 10000-15000rpm for 20-30min at room temperature, high pressure homogenizing at a pressure of 14-20MPa for 4-5min, high pressure homogenizing at a pressure of 27-32MPa for 4-5min, spray drying, controlling the inlet air temperature to 180-210°C, and the outlet air temperature to 80-100°C, to obtain a sodium hyaluronate and Tremella polysaccharide composition HAmella with beauty effects; In the prepared mixed solution, the dosage ratio of sodium hyaluronate, Tremella polysaccharide, deionized water, sucrose fatty acid ester, co-surfactant, grape seed oil, and dispersant is 50-60g:65-70g:4000-4500mL:200-220g:300-350g:260-300g:60-70g; The molecular weight of the sodium hyaluronate is 400-1200 kDa; The molecular weight of the Tremella polysaccharide is 200-600 kDa.

[0012] A sodium hyaluronate and tremella polysaccharide composition HAmella with cosmetic effects prepared by the aforementioned preparation method.

[0013] Compared with the prior art, the present invention has the following beneficial effects: (1) The preparation method of the sodium hyaluronate and Tremella polysaccharide composition HAmella with cosmetic effects of the present invention uses a dispersant and a co-surfactant in the preparation. The preparation method of the dispersant is to first mix citric acid and corn starch, spray dry, then cross-link the molecules under ultraviolet light, and then mix with calcium gluconate and wet grind. During the wet grinding process, citric acid and calcium ions in calcium gluconate are complexed, and calcium gluconate is further cross-linked with citric acid and corn starch, thereby promoting the cross-linking density between citric acid and corn starch cross-linked products to obtain the dispersant. After the dispersant is mixed with grape seed oil, the corn starch in the dispersant can play a role of pre-emulsification, and the complex between citric acid and calcium gluconate can play a role of stabilizing the grape seed oil, thereby reducing the oxidation of the grape seed oil and promoting the grape seed oil. Dispersion, promote the absorption of sodium hyaluronate and tremella polysaccharide by the digestive tract, improve the moisturizing, anti-oxidation and skin repair effects of the prepared sodium hyaluronate and tremella polysaccharide composition HAmella with cosmetic effects, in addition, the complex between citric acid and calcium gluconate can also reduce water absorption, improve the storage stability of the prepared sodium hyaluronate and tremella polysaccharide composition HAmella with cosmetic effects; the preparation method of the co-surfactant is to mix gum arabic with small molecule sodium hyaluronate, spray dry, and then cross-link the molecules under ultraviolet light to obtain a co-surfactant, and the small molecule sodium hyaluronate can improve the dispersing and emulsifying effects of gum arabic, and can further improve the moisturizing, anti-oxidation and skin repair effects of the prepared sodium hyaluronate and tremella polysaccharide composition HAmella with cosmetic effects; (2) The method for preparing the sodium hyaluronate and tremella polysaccharide composition HAmella with cosmetic effects of the present invention. The prepared sodium hyaluronate and tremella polysaccharide composition HAmella has the effects of moisturizing, anti-oxidation, and promoting skin repair. After continuous use of the sodium hyaluronate and tremella polysaccharide composition HAmella prepared by the present invention for 28 days, the average change rate of the moisture content of the stratum corneum of the skin is 30.4-31.9%, and the average change rate of the transepidermal water loss TEWL value of the skin is -18.6% to -16.6%. , the average skin hemoglobin EI value changed by -6.1% to -4.8%, the average skin elasticity R2 value changed by 6.8-8.5%, the average skin firmness parameter F4 value changed by -12.1% to -11.7%, the average cheek skin gloss changed by 10.3-11.0%, the average red area ratio changed by -40.3% to -39.7%, the average number of skin wrinkles changed by -30.6% to -25.0%, and the average skin wrinkle depth changed by -12.3% to -11.8%; (3) The method for preparing the sodium hyaluronate and tremella polysaccharide composition HAmella with cosmetic effects of the present invention, wherein the prepared sodium hyaluronate and tremella polysaccharide composition HAmella has a uniform particle size distribution; (4) The method for preparing the sodium hyaluronate and tremella polysaccharide composition HAmella with cosmetic effects of the present invention, wherein the prepared sodium hyaluronate and tremella polysaccharide composition HAmella has a fast dissolution rate in water. After mixing 10 g of the sodium hyaluronate and tremella polysaccharide composition HAmella with cosmetic effects prepared by the present invention with 150 mL of deionized water, stirring at room temperature at a stirring speed of 100 rpm for 1 min can achieve uniform mixing; (5) The method for preparing the sodium hyaluronate and tremella polysaccharide composition HAmella with cosmetic effects of the present invention has good storage stability. The sodium hyaluronate and tremella polysaccharide composition HAmella with cosmetic effects prepared by the present invention is placed in an environment with a temperature of 30°C and a relative humidity of 80% and left to stand for 1 day without any moisture problem. DETAILED DESCRIPTION

[0014] In order to have a clearer understanding of the technical features, purposes and effects of the present invention, the specific implementation methods of the present invention are now described.

[0015] Example 1 A method for preparing HAmella, a sodium hyaluronate and tremella polysaccharide composition with cosmetic effects, specifically comprising: 1. Prepare a dispersant: Mix 50g of citric acid and 600mL of deionized water, stir at room temperature at a stirring speed of 100rpm for 30min, adjust the pH to 3.5 with sodium hydroxide aqueous solution, add 50g of corn starch, continue stirring for 2h, let stand for 15h, spray dry, control the inlet air temperature to 160°C and the outlet air temperature to 60°C to obtain a mixed powder, grind the mixed powder to a particle size of less than 150 mesh, and then irradiate with an ultraviolet lamp with a wavelength of 320nm at 70°C for 2h, mix with 104g of calcium gluconate, ball mill at room temperature with a ball-to-water ratio of 5:1:0.4 and a rotation speed of 300rpm for 40min, dry at 120°C to obtain a mixed powder, grind the mixed powder to a particle size of less than 100 mesh, and obtain a dispersant; The mass concentration of the sodium hydroxide aqueous solution is 30%; 2. Preparation of a co-surfactant: 20 g of gum arabic, 14 g of sodium hyaluronate and 900 mL of deionized water were mixed, stirred at 50° C. at a stirring speed of 100 rpm for 1.5 h, spray dried, and the air inlet temperature was controlled at 160° C. and the air outlet temperature was controlled at 60° C. to obtain a mixed sodium hyaluronate, the mixed sodium hyaluronate was ground to a particle size of less than 100 mesh, and then irradiated with an ultraviolet lamp with a wavelength of 320 nm at 70° C. for 2 h to obtain a co-surfactant; The molecular weight of the sodium hyaluronate is 100 kDa; 3. Prepare a mixed solution: After mixing 50g of sodium hyaluronate, 65g of Tremella polysaccharide, and 4000mL of deionized water, ultrasonically oscillate at a frequency of 20kHz for 30min at room temperature, add 200g of sucrose fatty acid ester and 300g of co-surfactant, and continue ultrasonic oscillation for 30min to obtain a mixed aqueous phase; after mixing 260g of grape seed oil and 60g of dispersant, ultrasonically oscillate at a frequency of 20kHz for 20min at room temperature to obtain an oil phase; after mixing all the above mixed aqueous phase and all the above oil phases, high shear at a speed of 10000rpm for 20min at room temperature, high pressure homogenize at a pressure of 14MPa for 4min, high pressure homogenize at a pressure of 27MPa for 4min, spray dry, control the inlet temperature to 180°C, and the outlet temperature to 80°C, to obtain a sodium hyaluronate and Tremella polysaccharide composition HAmella with beauty effects; The molecular weight of the sodium hyaluronate is 1200 kDa; The molecular weight of the Tremella polysaccharide is 600kDa.

[0016] This embodiment also provides a sodium hyaluronate and Tremella polysaccharide composition HAmella with cosmetic effects prepared by the aforementioned preparation method.

[0017] Example 2 A method for preparing HAmella, a sodium hyaluronate and tremella polysaccharide composition with cosmetic effects, specifically comprising: 1. Prepare a dispersant: Mix 52g of citric acid and 650mL of deionized water, stir at room temperature at a stirring speed of 150rpm for 35min, adjust the pH to 3.8 with sodium hydroxide aqueous solution, add 55g of corn starch, continue stirring for 2.5h, let stand for 15.5h, spray dry, control the inlet air temperature to 180°C and the outlet air temperature to 80°C to obtain a mixed powder, grind the mixed powder to a particle size of less than 150 mesh, and then irradiate with an ultraviolet lamp with a wavelength of 320nm at 75°C for 2.5h, mix with 108g of calcium gluconate, ball mill at room temperature at a ball-to-water ratio of 5.5:1:0.4 and a rotation speed of 350rpm for 45min, dry at 130°C to obtain a mixed powder, grind the mixed powder to a particle size of less than 100 mesh, and obtain a dispersant; The mass concentration of the sodium hydroxide aqueous solution is 30%; 2. Preparation of a co-surfactant: 21 g of gum arabic, 15 g of sodium hyaluronate and 950 mL of deionized water were mixed, stirred at 55° C. at a stirring speed of 150 rpm for 1.5 h, spray dried, and the air inlet temperature was controlled at 180° C. and the air outlet temperature was controlled at 80° C. to obtain a mixed sodium hyaluronate, the mixed sodium hyaluronate was ground to a particle size of less than 100 mesh, and then irradiated at 75° C. with an ultraviolet lamp with a wavelength of 320 nm for 2.5 h to obtain a co-surfactant; The molecular weight of the sodium hyaluronate is 300 kDa; 3. Prepare a mixed solution: After mixing 55g of sodium hyaluronate, 68g of Tremella polysaccharide, and 4200mL of deionized water, ultrasonically oscillate at a frequency of 25kHz for 35min at room temperature, add 210g of sucrose fatty acid ester and 320g of co-surfactant, and continue ultrasonic oscillation for 35min to obtain a mixed aqueous phase; after mixing 280g of grape seed oil and 60-70g of dispersant, ultrasonically oscillate at a frequency of 25kHz for 25min at room temperature to obtain an oil phase; after mixing all the above mixed aqueous phase and all the above oil phases, high shear at a speed of 12000rpm for 25min at room temperature, high pressure homogenize at a pressure of 18MPa for 5min, high pressure homogenize at a pressure of 30MPa for 5min, spray dry, control the inlet temperature to 200°C, and the outlet temperature to 90°C, to obtain a sodium hyaluronate and Tremella polysaccharide composition HAmella with beauty effects; The molecular weight of the sodium hyaluronate is 800 kDa; The molecular weight of the Tremella polysaccharide is 400kDa.

[0018] This embodiment also provides a sodium hyaluronate and Tremella polysaccharide composition HAmella with cosmetic effects prepared by the aforementioned preparation method.

[0019] Example 3 A method for preparing HAmella, a sodium hyaluronate and tremella polysaccharide composition with cosmetic effects, specifically comprising: 1. Prepare a dispersant: Mix 55g of citric acid and 700mL of deionized water, stir at room temperature at a stirring speed of 200rpm for 40min, adjust the pH to 4 with sodium hydroxide aqueous solution, add 60g of corn starch, continue stirring for 3h, let stand for 16h, spray dry, control the inlet air temperature to 200℃, and the outlet air temperature to 90℃ to obtain a mixed powder, grind the mixed powder to a particle size of less than 150 mesh, and then irradiate with an ultraviolet lamp with a wavelength of 320nm at 80℃ for 2.5h, mix with 110g of calcium gluconate, ball mill at room temperature with a ball-to-water ratio of 6:1:0.5 and a rotation speed of 400rpm for 50min, dry at 140℃ to obtain a mixed powder, grind the mixed powder to a particle size of less than 100 mesh, and obtain a dispersant; The mass concentration of the sodium hydroxide aqueous solution is 30%; 2. Preparation of a co-surfactant: 22 g of gum arabic, 17 g of sodium hyaluronate and 1000 mL of deionized water were mixed, stirred at 60° C. at a stirring speed of 200 rpm for 2 h, spray dried, and the air inlet temperature was controlled at 200° C. and the air outlet temperature was controlled at 90° C. to obtain a mixed sodium hyaluronate, the mixed sodium hyaluronate was ground to a particle size of less than 100 mesh, and then irradiated at 80° C. with an ultraviolet lamp with a wavelength of 320 nm for 2.5 h to obtain a co-surfactant; The molecular weight of the sodium hyaluronate is 400 kDa; 3. Prepare a mixed solution: Mix 60g of sodium hyaluronate, 70g of Tremella polysaccharide, and 4500mL of deionized water, and ultrasonically vibrate at a frequency of 25kHz for 40min at room temperature, add 220g of sucrose fatty acid ester and 350g of co-surfactant, and continue ultrasonically vibrating for 40min to obtain a mixed aqueous phase; mix 300g of grape seed oil and 70g of dispersant, and ultrasonically vibrate at a frequency of 25kHz for 30min at room temperature to obtain an oil phase; mix all the above mixed aqueous phase and all the above oil phases, high shear at a speed of 15000rpm for 30min at room temperature, high pressure homogenize at a pressure of 20MPa for 5min, high pressure homogenize at a pressure of 32MPa for 5min, spray dry, control the inlet temperature to 210°C, and the outlet temperature to 100°C, to obtain a sodium hyaluronate and Tremella polysaccharide composition HAmella with beauty effects; The molecular weight of the sodium hyaluronate is 400 kDa; The molecular weight of the Tremella polysaccharide is 200 kDa.

[0020] This embodiment also provides a sodium hyaluronate and Tremella polysaccharide composition HAmella with cosmetic effects prepared by the aforementioned preparation method.

[0021] Comparative Example 1 In the method for preparing the sodium hyaluronate and tremella polysaccharide composition HAmella with cosmetic effects in Example 2, the first step of preparing the dispersant is omitted, and the addition of the dispersant is omitted in the third step of preparing the mixed solution, that is, 280 g of grape seed oil is prepared as the oil phase.

[0022] The remaining operations are the same as those in Example 2.

[0023] Comparative Example 2 In the method for preparing the sodium hyaluronate and Tremella polysaccharide composition HAmella with cosmetic effects in Example 2, the second step of preparing the co-surfactant is omitted, and in the third step of preparing the mixed solution, gum arabic or the like is used instead of the co-surfactant.

[0024] The remaining operations are the same as those in Example 2.

[0025] Performance Test 1 The particle size distribution of the sodium hyaluronate and Tremella polysaccharide composition HAmella with cosmetic effects prepared in Example 1-3 and Comparative Example 1-2 was observed, and the observation results were as follows:

[0026] Performance Test 2 10 g of the sodium hyaluronate and Tremella polysaccharide composition HAmella with cosmetic effects prepared in Examples 1-3 and Comparative Examples 1-2 were respectively mixed with 150 mL of deionized water, stirred at room temperature at a stirring speed of 100 rpm for 1 min, and then observed whether the mixture was uniformly mixed. The observation results were as follows:

[0027] Performance Test 3 The sodium hyaluronate and Tremella polysaccharide compositions HAmella with cosmetic effects prepared in Examples 1-3 and Comparative Examples 1-2 were placed in an environment with a temperature of 30° C. and a relative humidity of 80% for 1 day to observe whether there was a moisture problem. The observation results are as follows:

[0028] Performance Test 4 120 women aged 25-45 were recruited and the following conditions were met: loose and dull facial skin; fine lines or wrinkles at the corners of the eyes with wrinkle levels of 1-3; baseline moisture content of the stratum corneum <60 C.U.; transepidermal water loss (TEWL) greater than 12g / h / m 2; No serious systemic diseases, no immunodeficiency or autoimmune diseases; no skin treatment, cosmetic treatment or other tests that may affect the results; no active diseases; no allergic diseases; no use of hormone drugs and immunosuppressants in the past month; no participation in other clinical trials in the past month; The recruited women were randomly divided into 6 groups, 20 in each group, marked as groups 1-6, and the moisture content of the skin stratum corneum, the transepidermal water loss TEWL value, the skin hemoglobin EI value, the skin elasticity R2 value, the skin firmness parameter F4 value, the cheek skin glossiness, the red area ratio, the number of skin wrinkles, and the skin wrinkle depth of each woman were tested, and the average value of each group was calculated. The calculation results are as follows:

[0029] Then, the sodium hyaluronate and tremella polysaccharide composition HAmella with cosmetic effects prepared in Examples 1-3 and Comparative Examples 1-2 were respectively orally administered to groups 1-5 of women. Before oral administration, the sodium hyaluronate and tremella polysaccharide composition HAmella with cosmetic effects was mixed with water in a mass ratio of 1:15 and stirred evenly to obtain an oral solution, which was taken orally once a day, with 30 mL of the oral solution consumed each time. Six groups were used as blank controls, and no cosmetic products were used. After continuous drinking for 28 days, the moisture content of the stratum corneum, the transepidermal water loss TEWL value, the skin hemoglobin EI value, the skin elasticity R2 value, the skin firming parameter F4 value, the cheek skin gloss, the red zone area ratio, the number of skin wrinkles, and the skin wrinkle depth of each woman were tested, and the average value of each group was calculated. The calculation results are as follows:

[0030] The average change rate of skin stratum corneum moisture content, average change rate of skin transepidermal water loss TEWL value, average change rate of skin hemoglobin EI value growth rate, average change rate of skin elasticity R2 value, average change rate of skin firmness parameter F4 value, average change rate of cheek skin gloss, average change rate of red area ratio, average change rate of skin wrinkle number, and average change rate of skin wrinkle depth of each group were calculated, and the calculation results are as follows:

[0031] It can be seen from the results of performance tests 1-4 that, compared with the sodium hyaluronate and tremella polysaccharide composition HAmella with cosmetic effects prepared in Example 2, the sodium hyaluronate and tremella polysaccharide composition HAmella with cosmetic effects prepared in Comparative Example 1 has poor moisturizing, anti-oxidation, skin repair promoting effects, particle size distribution, dissolution rate in water, and storage resistance; the sodium hyaluronate and tremella polysaccharide composition HAmella with cosmetic effects prepared in Comparative Example 2 has poor moisturizing, anti-oxidation, skin repair promoting effects, particle size distribution, and dissolution rate in water; Unless otherwise specified, all percentages used in the present invention are by mass.

[0032] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for preparing a sodium hyaluronate and Tremella polysaccharide composition HAmella with cosmetic effects, characterized in that: include: preparing a dispersant, preparing a co-surfactant, and preparing a mixed solution; The dispersant is prepared by mixing citric acid and deionized water, stirring at room temperature, adjusting the pH to 3.5-4, adding corn starch, continuing stirring, standing, spray drying to obtain a mixed powder, grinding the mixed powder, and then irradiating it with an ultraviolet lamp at 70-80° C., mixing it with calcium gluconate, ball milling it at room temperature, drying it to obtain a mixed powder, and grinding the mixed powder to obtain a dispersant; The co-surfactant is prepared by mixing gum arabic, sodium hyaluronate and deionized water, stirring at 50-60° C., spray drying to obtain mixed sodium hyaluronate, grinding the mixed sodium hyaluronate, and then irradiating with ultraviolet light at 70-80° C. to obtain the co-surfactant.

2. The method for preparing the sodium hyaluronate and Tremella polysaccharide composition HAmella with cosmetic effects according to claim 1, characterized in that: In the preparation of the dispersant, the dosage ratio of citric acid, deionized water, corn starch and calcium gluconate is 50-55g:600-700mL:50-60g:104-110g; The wavelength of the ultraviolet lamp is 320nm; The ball-to-material-water ratio in the wet grinding is 5-6:1:0.4-0.5, and the rotation speed is 300-400rpm.

3. The method for preparing the sodium hyaluronate and Tremella polysaccharide composition HAmella with cosmetic effects according to claim 1, characterized in that: In the preparation of the cosurfactant, the dosage ratio of gum arabic, sodium hyaluronate and deionized water is 20-22 g: 14-17 g: 900-1000 mL; The molecular weight of the sodium hyaluronate is 100-400 kDa; The wavelength of the ultraviolet lamp is 320nm.

4. The method for preparing the sodium hyaluronate and Tremella polysaccharide composition HAmella with cosmetic effects according to claim 1, characterized in that: The mixed solution is prepared by uniformly mixing sodium hyaluronate, tremella polysaccharide and deionized water, adding sucrose fatty acid ester and a co-surfactant, and mixing uniformly to obtain a mixed aqueous phase; uniformly mixing grape seed oil and a dispersant to obtain an oil phase; after mixing the mixed aqueous phase and the oil phase, high shearing is performed at room temperature, and then primary homogenization and secondary homogenization are performed, and then spray drying is performed to obtain a sodium hyaluronate and tremella polysaccharide composition HAmella with cosmetic effects.

5. The method for preparing the sodium hyaluronate and tremella polysaccharide composition HAmella with cosmetic effects according to claim 4, characterized in that: In the prepared mixed solution, the dosage ratio of sodium hyaluronate, Tremella polysaccharide, deionized water, sucrose fatty acid ester, co-surfactant, grape seed oil, and dispersant is 50-60g:65-70g:4000-4500mL:200-220g:300-350g:260-300g:60-70g; The molecular weight of the sodium hyaluronate is 400-1200 kDa; The molecular weight of the Tremella polysaccharide is 200-600 kDa; The primary homogenization pressure is 14-20 MPa and the time is 4-5 min; The secondary homogenization pressure is 27-32 MPa and the time is 4-5 min.

6. A sodium hyaluronate and Tremella polysaccharide composition HAmella with cosmetic effects prepared by the preparation method according to any one of claims 1 to 5.

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