Degradation method of aflatoxin in Chinese herbal medicine extract

Abstract

The invention belongs to the technical field of aflatoxin degradation, and provides a method for degrading aflatoxin in a Chinese herbal medicine extract. The preparation method comprises the following steps: mixing a hydrogen peroxide solution, carbonate, ammonia water and hydroxide to obtain a mixed reagent; mixing the Chinese herbal medicine extract containing aflatoxin, the mixed reagent and water to obtain an intermediate product; and drying the intermediate product to obtain the aflatoxin-removed Chinese herbal medicine extract. According to the method, the degradation effect and stability of aflatoxin in the Chinese herbal medicine extract are improved, the technological process is simplified, the cost is reduced, and a more convenient and efficient solution is provided for the Chinese herbal medicine industry in China.

Description

Technical Field

[0001] The invention relates to the technical field of aflatoxin degradation, and in particular to a method for degrading aflatoxin in a Chinese herbal medicine extract. Background Art

[0002] In the production process of Chinese herbal extracts, aflatoxin is a common contaminant, which seriously affects the quality and safety of Chinese herbal products.

[0003] In the prior art, microbial enzyme preparations are usually used to degrade aflatoxins. Such as microorganisms such as Bacillus subtilis, Aspergillus niger and Aspergillus oryzae. These microorganisms work together to effectively degrade aflatoxins in Chinese herbal medicine extracts. However, in practical applications, microbial enzyme preparations have problems such as unstable degradation effects and difficulty in preparing microbial enzyme preparations. In addition, there are technical solutions that use plant extracts such as tea extracts, green tea extracts, etc. to degrade aflatoxins through mechanisms such as anti-oxidation and inhibition of enzyme activity, but the selection and application conditions of plant extracts are relatively complicated and the cost is relatively high.

[0004] The present invention proposes a method for degrading aflatoxin in a Chinese herbal medicine extract, which improves the degradation effect and stability of aflatoxin in the Chinese herbal medicine extract, simplifies the process flow, reduces costs, and provides a more convenient and efficient solution for my country's Chinese herbal medicine industry. Summary of the invention

[0005] The invention provides a method for degrading aflatoxin in a Chinese herbal medicine extract, which is used to solve the defects of poor and unstable degradation effect of aflatoxin in a Chinese herbal medicine extract and complicated process in the prior art.

[0006] The present invention provides a method for degrading aflatoxin in a Chinese herbal medicine extract, comprising the following steps:

[0007] Mixing hydrogen peroxide solution, carbonate, ammonia water and hydroxide to obtain a mixed reagent;

[0008] Adding a mixed reagent and water to the Chinese herbal medicine extract containing aflatoxin, homogenizing and emulsifying to obtain an intermediate product;

[0009] The intermediate product is dried to obtain aflatoxin-free Chinese herbal medicine extract.

[0010] According to a method for degrading aflatoxin in a Chinese herbal medicine extract provided by the present invention, the concentration of the hydrogen peroxide solution is 35-50%, and the concentration of the ammonia water is 25-30%.

[0011] According to a method for degrading aflatoxin in a Chinese herbal medicine extract provided by the present invention, the mass ratio of the hydrogen peroxide solution, carbonate, ammonia water, and hydroxide is 2-8:0.1-4:0.1-2:0.1-4.

[0012] According to a method for degrading aflatoxin in a Chinese herbal medicine extract provided by the present invention, the mass volume ratio of the aflatoxin Chinese herbal medicine extract, the mixed reagent and water is 200g:0.2-10g:400mL.

[0013] According to the method for degrading aflatoxin in a Chinese herbal medicine extract provided by the present invention, the carbonate is sodium bicarbonate or sodium carbonate.

[0014] According to the method for degrading aflatoxin in a Chinese herbal medicine extract provided by the present invention, the hydroxide is any one of sodium hydroxide, calcium hydroxide and potassium hydroxide.

[0015] According to a method for degrading aflatoxin in a Chinese herbal medicine extract provided by the present invention, the Chinese herbal medicine extract includes astragalus extract, kudzu root extract, wolfberry extract, yam extract, and loquat leaf extract.

[0016] According to a method for degrading aflatoxin in a Chinese herbal medicine extract provided by the present invention, the homogenization and emulsification is carried out under ultrasonic conditions, the ultrasonic frequency is 20-40 kHz, the ultrasonic power is 200-400 W, and the ultrasonic time is 1-2 hours.

[0017] According to the method for degrading aflatoxin in a Chinese herbal medicine extract provided by the present invention, the drying treatment is any one of spray dryer drying, microwave dryer drying, and freeze dryer drying.

[0018] According to a method for degrading aflatoxin in a Chinese herbal medicine extract provided by the present invention, the inlet air temperature of the spray dryer is 140-200°C, the outlet air temperature is 80-110°C, and the temperature inside the tower is 80-92°C; the drying temperature of the microwave dryer is 50-80°C; and the drying temperature of the freeze dryer is ≤40°C.

[0019] The method for degrading aflatoxin in a Chinese herbal medicine extract provided by the present invention comprises the following steps: mixing hydrogen peroxide solution, carbonate, ammonia water and hydroxide to obtain a mixed reagent; mixing a Chinese herbal medicine extract containing aflatoxin, the mixed reagent and water to obtain an intermediate product; and drying and sieving the intermediate product to obtain a Chinese herbal medicine extract free of aflatoxin. The method solves the technical problems of poor degradation effect and instability of aflatoxin in Chinese herbal medicine extracts and complex processes in the prior art, and achieves the beneficial effects of improving the degradation effect and stability of aflatoxin in Chinese herbal medicine extracts, simplifying the process flow and reducing costs. DETAILED DESCRIPTION

[0020] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] The present invention provides a method for degrading aflatoxin in a Chinese herbal medicine extract, comprising the following steps:

[0022] Mixing hydrogen peroxide solution, carbonate, ammonia water and hydroxide to obtain a mixed reagent;

[0023] Adding a mixed reagent and water to the Chinese herbal medicine extract containing aflatoxin, homogenizing and emulsifying to obtain an intermediate product;

[0024] The intermediate product is dried to obtain aflatoxin-free Chinese herbal medicine extract.

[0025] In the present invention, the concentration of the hydrogen peroxide solution is 35-50%, preferably 38-47%, more preferably 40-44%, and the concentration of the ammonia water is 25-30%, preferably 27-28%.

[0026] In the present invention, the mass ratio of the hydrogen peroxide solution, carbonate, ammonia water and hydroxide is 2-8:0.1-4:0.1-2:0.1-4, preferably 4-6:1-3:0.5-1.5:1-3, and more preferably 5:2:1:2.

[0027] In the present invention, the mass volume ratio of the aflatoxin Chinese herbal extract, the mixed reagent and water is 200g:0.2-10g:400mL, preferably 200g:2-8g:400mL, and more preferably 200g:4-6g:400mL.

[0028] In the present invention, the carbonate is sodium bicarbonate or sodium carbonate, preferably sodium carbonate.

[0029] In the present invention, the hydroxide is any one of sodium hydroxide, calcium hydroxide and potassium hydroxide, preferably any one of sodium hydroxide or potassium hydroxide, and more preferably potassium hydroxide.

[0030] In the present invention, the Chinese herbal medicine extracts include astragalus extract, kudzu root extract, wolfberry extract, yam extract, and loquat leaf extract.

[0031] In the present invention, the homogenization emulsification is carried out under ultrasonic conditions, the frequency of the ultrasound is 20 to 40 kHz, preferably 25 to 35 kHz, and more preferably 28 to 32 kHz, the power of the ultrasound is 200 to 400 W, preferably 250 to 350 W, and more preferably 280 to 320 W, and the time of the ultrasound is 1 to 2 hours, preferably 1.2 to 1.8 hours, and more preferably 1.4 to 1.6 hours.

[0032] In the present invention, the drying treatment is any one of spray dryer drying, microwave dryer drying, and freeze dryer drying.

[0033] In the present invention, the air inlet temperature of the spray dryer is 140-200°C, preferably 150-190°C, and more preferably 160-180°C, the air outlet temperature is 80-110°C, preferably 90-100°C, and the temperature in the tower is 80-92°C, preferably 84-88°C; the drying temperature of the microwave dryer is 50-80°C, preferably 60-70°C; the drying temperature of the freeze dryer is ≤40°C.

[0034] Example 1

[0035] 5g of 40% hydrogen peroxide solution, 2g of sodium carbonate, 1g of 27% ammonia water and 2g of potassium hydroxide were mixed to obtain a mixed reagent. The mixed reagent and 800mL of water were added to 400g of aflatoxin-containing astragalus extract, and homogenized and emulsified for 1.5 hours under ultrasonic conditions of 30kHz and 300W to obtain an intermediate product. The spray dryer was set to dry at an air inlet temperature of 200°C, an air outlet temperature of 100°C, and a tower temperature of 88°C, and the intermediate product was dried to obtain aflatoxin-free astragalus extract.

[0036] After testing, the aflatoxin content in the aflatoxin-free Astragalus extract was 0.016 ppm.

[0037] Example 2

[0038] 5g of 40% hydrogen peroxide solution, 2g of sodium carbonate, 1g of 27% ammonia water and 2g of potassium hydroxide were mixed to obtain a mixed reagent. The mixed reagent and 800mL of water were added to 400g of aflatoxin-containing kudzu root extract, and homogenized and emulsified for 1.5 hours under ultrasonic conditions of 30kHz and 300W to obtain an intermediate product. The spray dryer was set to dry with an inlet air temperature of 200°C, an outlet air temperature of 100°C, and an internal temperature of 88°C, and the intermediate product was dried to obtain a kudzu root extract free of aflatoxin.

[0039] After testing, the aflatoxin content in the aflatoxin-free Pueraria extract was 0.003 ppm.

[0040] Example 3

[0041] 5g of 40% hydrogen peroxide solution, 2g of sodium carbonate, 1g of 27% ammonia water and 2g of potassium hydroxide were mixed to obtain a mixed reagent. The mixed reagent and 800mL of water were added to 400g of aflatoxin-containing wolfberry extract, and homogenized and emulsified for 1.5 hours under ultrasonic conditions of 30kHz and 300W to obtain an intermediate product. The spray dryer was set to dry at an inlet temperature of 200°C, an outlet temperature of 100°C, and a temperature in the tower of 88°C, and the intermediate product was dried to obtain aflatoxin-free wolfberry extract.

[0042] After testing, the aflatoxin content in the deaflatoxinized wolfberry extract was 0.005 ppm.

[0043] Example 4

[0044] 5g of 40% hydrogen peroxide solution, 2g of sodium carbonate, 1g of 27% ammonia water and 2g of potassium hydroxide were mixed to obtain a mixed reagent. The mixed reagent and 800mL of water were added to 400g of yam extract containing aflatoxin, and homogenized and emulsified for 1.5 hours under ultrasonic conditions of 30kHz and 300W to obtain an intermediate product. The spray dryer was set to dry with an inlet air temperature of 200°C, an outlet air temperature of 100°C, and an internal temperature of 88°C, and the intermediate product was dried to obtain a yam extract free of aflatoxin.

[0045] After testing, the aflatoxin content in the aflatoxin-free yam extract was 0.004 ppm.

[0046] Example 5

[0047] 5g of 40% hydrogen peroxide solution, 2g of sodium carbonate, 1g of 27% ammonia water and 2g of potassium hydroxide were mixed to obtain a mixed reagent. The mixed reagent and 800mL of water were added to 400g of loquat leaf extract containing aflatoxin, and homogenized and emulsified for 1.5 hours under ultrasonic conditions of 30kHz and 300W to obtain an intermediate product. The spray dryer was set to dry at an inlet temperature of 200°C, an outlet temperature of 100°C, and a temperature in the tower of 88°C, and the intermediate product was dried to obtain a loquat leaf extract free of aflatoxin.

[0048] After testing, the aflatoxin content in the aflatoxin-free loquat leaf extract was 0.003 ppm.

[0049] Example 6

[0050] 5 g of 40% hydrogen peroxide solution, 2 g of sodium bicarbonate, 1 g of 27% ammonia water and 2 g of sodium hydroxide were mixed to obtain a mixed reagent. The mixed reagent and 800 mL of water were added to 400 g of aflatoxin-containing astragalus extract, and homogenized and emulsified for 1.5 hours under ultrasonic conditions of 30 kHz and 300 W to obtain an intermediate product. The drying temperature of the microwave dryer was set to 65° C., and the intermediate product was dried to obtain aflatoxin-free astragalus extract.

[0051] After testing, the aflatoxin content in the deaflatoxinized Astragalus extract was 0.021 ppm.

[0052] Example 7

[0053] 5 g of 40% hydrogen peroxide solution, 2 g of calcium carbonate, 1 g of 27% ammonia water and 2 g of potassium hydroxide were mixed to obtain a mixed reagent. The mixed reagent and 800 mL of water were added to 400 g of aflatoxin-containing astragalus extract, and homogenized and emulsified for 1.5 hours under ultrasonic conditions of 30 kHz and 300 W to obtain an intermediate product. The drying temperature of the freeze dryer was set to 40° C., and the intermediate product was dried to obtain aflatoxin-free astragalus extract.

[0054] After testing, the aflatoxin content in the deaflatoxinized Astragalus extract was 0.035 ppm.

[0055] The aflatoxin content of the aflatoxin-containing Chinese herbal medicine extracts used in the above examples was tested. The aflatoxin content in the astragalus extract containing aflatoxin was 8900 ppm, the aflatoxin content in the kudzu root extract containing aflatoxin was 5400 ppm, the aflatoxin content in the wolfberry extract containing aflatoxin was 3700 ppm, the aflatoxin content in the yam extract containing aflatoxin was 2300 ppm, and the aflatoxin content in the loquat leaf extract containing aflatoxin was 1200 ppm.

[0056] According to statistics, the degradation effects of aflatoxin of Examples 1 to 7 are shown in Table 1.

[0057] Table 1. Degradation effect of aflatoxin in Examples 1 to 7

[0058]

[0059] It can be seen that the methods for degrading aflatoxin in Chinese herbal extracts provided in Examples 1 to 7 of the present invention can efficiently and stably degrade aflatoxin in Chinese herbal extracts, and the degradation rates of aflatoxin in Chinese herbal extracts are all higher than 99.99%. In addition, the process flow is simple and the cost is low.

[0060] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for degrading aflatoxin in a Chinese herbal medicine extract, characterized in that: The following steps are involved: Mixing hydrogen peroxide solution, carbonate, ammonia water and hydroxide to obtain a mixed reagent; Adding a mixed reagent and water to the Chinese herbal medicine extract containing aflatoxin, homogenizing and emulsifying to obtain an intermediate product; The intermediate product is dried to obtain aflatoxin-free Chinese herbal medicine extract.

2. The method for degrading aflatoxin in a Chinese herbal medicine extract according to claim 1, characterized in that: The concentration of the hydrogen peroxide solution is 35-50%, and the concentration of the ammonia water is 25-30%.

3. The method for degrading aflatoxin in a Chinese herbal medicine extract according to claim 1, characterized in that: The mass ratio of the hydrogen peroxide solution, carbonate, ammonia water and hydroxide is 2-8:0.1-4:0.1-2:0.1-4.

4. The method for degrading aflatoxin in a Chinese herbal medicine extract according to claim 1, characterized in that: The mass volume ratio of the aflatoxin Chinese herbal extract, the mixed reagent and water is 200g:0.2-10g:400mL.

5. The method for degrading aflatoxin in a Chinese herbal medicine extract according to claim 1, characterized in that: The carbonate is sodium bicarbonate or sodium carbonate.

6. The method for degrading aflatoxin in a Chinese herbal medicine extract according to claim 1, characterized in that: The hydroxide is any one of sodium hydroxide, calcium hydroxide and potassium hydroxide.

7. The method for degrading aflatoxin in a Chinese herbal medicine extract according to claim 1, characterized in that: The Chinese herbal medicine extracts include astragalus extract, kudzu root extract, wolfberry extract, yam extract, and loquat leaf extract.

8. The method for degrading aflatoxin in a Chinese herbal medicine extract according to claim 1, characterized in that: The homogenization and emulsification is carried out under ultrasonic conditions, the ultrasonic frequency is 20-40 kHz, the ultrasonic power is 200-400 W, and the ultrasonic time is 1-2 hours.

9. The method for degrading aflatoxin in a Chinese herbal medicine extract according to claim 1, characterized in that: The drying treatment is any one of spray dryer drying, microwave dryer drying, and freeze dryer drying.

10. The method for degrading aflatoxin in a Chinese herbal medicine extract according to claim 9, characterized in that: The inlet air temperature of the spray dryer is 140-200°C, the outlet air temperature is 80-110°C, and the temperature inside the tower is 80-92°C; the drying temperature of the microwave dryer is 50-80°C; and the drying temperature of the freeze dryer is ≤40°C.

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