Light reflection assembly for prolonging optical path of colorimetric pool

Abstract

The invention relates to a divisional application of which the application number is 201911198047.2. The embodiment of the invention discloses a light reflection assembly for prolonging the optical path of a colorimetric pool. The light reflection assembly comprises a first reflection part and a second reflection part which are matched with each other, the first reflection part and the second reflection part are respectively provided with a plurality of reflection mirror surface groups, and the reflection mirror surface groups can be arranged on a parallel surface opposite to the light-transmitting surface of the system to be detected in a rotary combination manner at any angle by taking corresponding incident light as an axis. Through the reflection of the reflection mirror group between the two reflection parts, the optical path of the initial incident light in the system to be measured is increased. The method has the beneficial effects that 1, under the condition of not changing the optical path size and appearance of the existing optical measurement instrument equipment, the measurement optical path of a system to be measured is increased, the detection limit of the existing method is reduced, and the sensitivity is improved; 2, due to the reflection effect between the two reflection parts, the light incident direction and the light emergent direction are both perpendicular to a system to be measured, and an existing optical measuring instrument can be matched with the light reflection assembly through simple light path adjustment.

Description

[0001] This application is a divisional application. The application number of the original application is 201911198047.2, the application date is November 29, 2019, and the name of the invention is “A light reflecting component for extending the optical path”. Technical Field

[0002] The invention belongs to the field of optical measurement, and in particular relates to a light reflection component for extending the optical path of a colorimetric cell. Background Art

[0003] Lambert-Beer law is the basis of many optical analysis methods. Its quantitative analysis is based on the fact that the measured absorbance A is proportional to the concentration c of the absorbing substance and the thickness b (optical path) of the absorbing layer. The analysis method based on this law is widely used in the fields of environment, biological hygiene, water conservancy, etc. It has the characteristics of low instrument and equipment cost, relatively simple operation, high accuracy of results and good reproducibility.

[0004] The optical path length has an important influence on the sensitivity and detection limit of the analysis method based on the Lambert-Beer law. The optical path length of the cuvette or flow optical detection cell of the common traditional spectrophotometric analysis is mostly fixed in the range of 1 to 3 cm. Samples with small absorption coefficients or low concentrations are often difficult to measure, which greatly limits the range of types and concentrations of substances detected by traditional spectrophotometric analysis.

[0005] Microplate reader, also commonly known as microplate reader, is a conventional instrument for measuring enzyme-linked immunosorbent assay. It is a disguised professional spectrophotometer. The biggest difference between it and traditional spectrophotometric analysis is that the volume of the absorbance cell is small (microliter order) and the direction of the light path is perpendicular to the liquid surface of the solution. This makes the optical path length of the microplate reader lower than that of traditional spectrophotometric analysis, which greatly limits the application scope of the photometric analysis of the microplate reader. In the measurement activities of the immersion full-spectrum analyzer, since the instrument measurement optical path length is fixed, for the case where the concentration of the test object in the water body to be tested is low, it can only be achieved by increasing the instrument optical path length at this stage, which greatly increases the manufacturing cost and weight of the instrument and reduces the portability and universality of the instrument. Gas optical detection also faces the problem of limited optical path length. At this stage, most of them can only partially solve the problem through high-concentration standard gas calibration and long-distance detection. Summary of the invention

[0006] In order to solve the above problems, the purpose of the present invention is to provide a light reflection component for extending the optical path of a colorimetric cell, which can effectively increase the optical path of light in the system to be measured, thereby reducing the detection limit of the system to be measured and improving the detection sensitivity, and has good practicality.

[0007] The present invention provides the following technical solutions: A light reflecting component for extending the optical path of a colorimetric cell, characterized in that the light reflecting component comprises a first reflecting portion and a second reflecting portion, the first reflecting portion and the second reflecting portion are respectively arranged on both sides of a light-transmitting surface of a system to be measured; the first reflecting portion comprises m groups of first reflecting mirror surfaces, the second reflecting portion comprises n groups of second reflecting mirror surfaces, m is an integer greater than or equal to 1, and n is an integer greater than or equal to 0; the first reflecting mirror surface group and the second reflecting mirror surface group are both formed by two reflecting mirror surfaces intersecting at an angle of 90 degrees, and the 90-degree angle formed by the two reflecting mirror surfaces is arranged toward the system to be measured, and the angle bisector plane is perpendicular to the light-transmitting surface of the system to be measured; The first reflecting mirror surface group of the first reflecting part and the second reflecting mirror surface group of the second reflecting part are arranged in a staggered manner relative to each other, so that the initial incident light moves forward in sequence under the reflection of the first reflecting mirror surface group and the second reflecting mirror surface group.

[0008] As a further technical solution of the present invention, the first reflective mirror group on the first reflective part and the second reflective mirror group on the second reflective part can be rotated at different angles on a surface parallel to the light-transmitting surface of the system to be measured with the corresponding incident light as the axis.

[0009] As a further technical solution of the present invention, the light reflecting assembly is provided with a light entrance hole / slit and a light exit hole / slit, and the light entrance hole / slit and the light exit hole / slit are both arranged in the second reflecting part or respectively in the second reflecting part and the first reflecting part.

[0010] As a preferred technical solution of the present invention, the light reflection components can be either a single set or multiple sets and positioned inside the instrument optical path detection system for use.

[0011] Multiple sets of light reflection components built into the instrument's optical path detection system can be manually or automatically replaced to meet different optical path requirements.

[0012] As a preferred technical solution of the present invention, the initial incident light source of the light reflecting component is a point light source or a line light source.

[0013] As a further technical solution of the present invention, the system to be tested may be in liquid or gaseous form.

[0014] As a further technical solution of the present invention, the optical path length depends on the vertical width of the system to be measured between the first reflecting part and the second reflecting part and the number of times the light is reflected in the system to be measured.

[0015] As a further technical solution of the present invention, the light reflecting assembly may include a first reflecting part, a second reflecting part, and a fixed supporting frame connecting the two reflecting parts. The fixed supporting frame is U-shaped or a hollow open rectangular parallelepiped with three sides connected, and is mounted on the periphery of the container of the system to be tested during detection.

[0016] The beneficial effects of the present invention are: 1. By arranging the first reflecting part and the second reflecting part that cooperate with each other on both sides of the light-transmitting surface of the system to be measured, when the light enters the system to be measured, the measurement optical path is effectively increased after multiple reflections. Without changing the appearance and size of the existing optical measuring instrument, the detection limit of the existing method is reduced and the detection sensitivity is improved.

[0017] 2. The reflective mirror group arranged on the first reflective part and the second reflective part makes the directions of the incident light and the emitted light perpendicular to the system to be measured. The existing optical measuring instruments and equipment can match the light reflection component by slightly adjusting the optical path system.

[0018] 3. The present invention increases the measuring optical path by providing a reflective mirror group that cooperates with each other to increase the optical path through light reflection. The reflective mirror group does not directly contact the system to be measured, which reduces the possibility of damage to the mirror surface and has strong practicality. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1a and 1b It is a structural diagram of the light emitting assembly of the present invention used for conventional cuvette detection; Figure 2a and 2b It is a structural diagram of the light emitting assembly of the present invention used for flow-type optical detection; Figure 3 It is a structural diagram of the light emitting assembly of the present invention used for detection by a microplate detector; Figure 4a and 4b It is a structural diagram of the light emitting assembly of the present invention used for detection by an immersion full spectrometer; Figure 5a and 5b is a structural diagram of the light emitting assembly of the present invention used for gas optical detection; Figure 6a and 6b Schematic diagram of the direction of light in the reflector surface groups of two combined structures; Figure 7 Schematic diagram of the light path of the light emitting component of the present invention for a rectangular light-transmitting surface. The center point of ⊙ is the light entry point, the center point of X is the light exit point, and L i is the initial incident point of the light, L e is the final exit point of the light, and the dotted line represents the sequence of the incoming and outgoing light; Figure 8 The light emitting assembly of the present invention is used for the light path of a circular light-transmitting surface. The center point of ⊙ is the light entry point, the center point of X is the light exit point, and L i is the initial incident point of the light, L eis the final exit point of the light, and the dotted line represents the sequence of the incoming and outgoing light; The meanings of the symbols in the accompanying drawings are as follows: 1. First reflecting part; 2. Second reflecting part; 3. First reflecting mirror group; 4. Second reflecting mirror group; 5. Hole / slit for light to enter; 6. Hole / slit for light to exit; DETAILED DESCRIPTION

[0020] The present invention is described in detail below with reference to specific embodiments.

[0021] Example 1 like Figure 1a and 1b As shown, a cuvette light reflection assembly with a long optical path includes a first reflection part 1, a second reflection part 2, a light entry hole / slit 5 and a light exit hole / slit 6. The first reflection part 1 is provided with a plurality of first reflection mirror groups 3 corresponding to each other, and the second reflection part 2 is provided with a plurality of second reflection mirror groups 4 corresponding to each other.

[0022] like Figure 1a As shown, the number of the first reflecting mirror surface group 3 on the first reflecting part 1 of the cuvette light reflecting assembly is one more than the number of the second reflecting mirror surface group 4 provided on the second reflecting part 2. After the light enters from the light entrance hole / slit 5 on the second reflecting part 2, it is reflected by the first reflecting mirror surface group 3 on the first reflecting part 1, and then reflected to the second reflecting mirror surface group 4 on the second reflecting part 2, and then continues to reflect in sequence, and finally the light is emitted from the light exit hole / slit 6 located on the second reflecting part 2, at which time the initial incident light and the final exit light are located on the same side of the solution.

[0023] like Figure 1b As shown, the number of the first reflecting mirror surface groups 3 on the first reflecting part 1 of the cuvette light reflecting assembly is the same as the number of the second reflecting mirror surface groups 4 provided on the second reflecting part 2. After the light enters from the light entrance hole / slit 5 on the second reflecting part 2, it is reflected by the first reflecting mirror surface group 3 on the first reflecting part 1, and then reflected to the second reflecting mirror surface group 4 on the second reflecting part 2, and then continues to reflect in sequence, and finally the light is emitted from the light exit hole / slit 6 located on the first reflecting part 1, and the initial incident light and the final exit light are located on opposite sides of the solution.

[0024] In this example, the cuvette light reflection component can be fixedly built into the optical path detection system, or can be designed to be externally mounted such as in the form of a cuvette cover.

[0025] Example 2 like Figure 2a , 2bAs shown, a flow-type cuvette cell and a light emitting assembly for a flow-type optical detection system. The light emitting assembly includes a first reflecting portion 1, a second reflecting portion 2, a light entry hole / slit 5, and a light exit hole / slit 6. The first reflecting portion 1 is provided with a plurality of first reflecting mirror groups 3 corresponding to each other, and the second reflecting portion 2 is provided with a plurality of second reflecting mirror groups 4 corresponding to each other.

[0026] like Figure 2a As shown, a flow-type cuvette cell for a flow-type optical detection system has an outlet and an inlet that penetrate the system to be measured, and the cross-section of the cuvette cell is rectangular. The first reflecting part 1 and the second reflecting part 2 of the light reflecting assembly are respectively arranged on both sides of the light-transmitting pool wall of the cross-section to be measured of the flow-type cuvette cell. The number of the first reflecting mirror surface groups 3 on the first reflecting part 1 of the cuvette cell light reflecting assembly is the same as the number of the second reflecting mirror surface groups 4 arranged on the second reflecting part 2. After the point light source is incident from the light incident hole / slit 5 on the second reflecting part 2, it is reflected by the first reflecting mirror surface group 3 on the first reflecting part 1 to the second reflecting mirror surface group 4 on the second reflecting part 2, and continues to reflect in sequence, and finally the light is emitted from the light exit hole / slit 6 located on the first reflecting part 1, and the initial incident light and the final exit light are located on both sides of the solution to be measured.

[0027] like Figure 2b As shown, Figure 2a The light emitting assembly for flow optical detection shown is different, in that the number of the first reflecting mirror surface group 3 on the first reflecting part 1 of the cuvette light reflecting assembly is one more than the number of the second reflecting mirror surface group 4 provided on the second reflecting part 2. The light entrance hole / slit 5 and the light exit hole / slit 6 are both on the second reflecting part 2, and the initial incident light and the final exit light are located on the same side of the solution.

[0028] The light emitting assembly of the present invention can be built into the light path detection system in a single set or multiple sets. When multiple sets are built into the light path detection system, different optical path lengths can be achieved by rotating the light reflecting assemblies corresponding to different pairs. The cross section of the flow-type cuvette pool can be square, circular or other shapes, which can be adjusted by those skilled in the art according to actual needs.

[0029] When the system to be tested flows in the flow cuvette pool, the concentration will change along the flow direction of the liquid. The present invention arranges a first reflective mirror group 3 and a second reflective mirror group 4 which are distributed correspondingly to each other on both sides of the light-transmitting wall of the cuvette pool. When the light enters the flow cuvette pool at a certain cross section perpendicular to the flow direction of the solution, after multiple reflections on the same cross section, the concentration change can be captured more keenly, thereby improving the sensitivity and accuracy of the detection.

[0030] Example 3 like Figure 3As shown, a light emitting component for extended light path measurement in a microplate detector.

[0031] The first reflective part 1 and the second reflective part 2 of the light reflecting assembly are respectively fixed in the optical path detection system of the microplate detector, and are located on both sides of the microplate solution. The optical detection of the solutions in different microwells in the microplate is achieved by moving the microplate.

[0032] In this embodiment, the first reflecting part 1 and the second reflecting part 2 are respectively provided with a first reflecting mirror group 3 and a second reflecting mirror group 4 distributed correspondingly to each other, so that light can enter along a direction perpendicular to the solution in the microwells and then be emitted perpendicular to the direction of the solution in the microwells.

[0033] The light reflection components can be a single set or multiple sets built into the optical path detection system of the microplate detector. Different optical path lengths can be achieved by rotating the light reflection components corresponding to different pairs.

[0034] Example 4 like Figure 4a and 4b As shown, a light emitting component for extended optical path measurement of an immersion full spectrum detector.

[0035] The first reflective part 1 and the second reflective part 2 of the light reflection component are respectively fixed in the optical path detection system of the immersion full spectrum detector, located on both sides of the water / aqueous solution to be tested, and sealed in an optically transparent sealed window. Through the first reflective mirror group 3 and the second reflective mirror group 4 distributed correspondingly to each other, light can be injected perpendicularly to the direction of the water / aqueous solution to be tested, and then emitted perpendicularly to the water / aqueous solution.

[0036] The light reflection components can be built into the optical path detection system of the immersion full-spectrum detector in a single set or in multiple sets. Different optical path lengths can be achieved by rotating the light reflection component sets corresponding to different pairs.

[0037] Example 5 like Figure 5a and 5b Shown is a light emitting assembly for gas optical extended light path detection.

[0038] The first reflecting part 1 and the second reflecting part 2 of the light reflecting assembly are respectively fixed in the optical path detection system of the gas optical detector, and are located on both sides of the light-transmitting surface of the gas to be detected or its container / cell.

[0039] In this embodiment, the first reflecting part 1 and the second reflecting part 2 are respectively provided with a first reflecting mirror group 3 and a second reflecting mirror group 4 distributed correspondingly to each other, so that light can be incident along a direction perpendicular to the gas to be measured and then emitted perpendicular to the direction of the gas to be measured.

[0040] The light reflection components can be a single set or multiple sets positioned in the gas optical detection light path system, and different optical path lengths can be achieved by rotating the light reflection components corresponding to different pairs.

[0041] Example 6 Figure 6a and 6b Schematic diagram of light direction of two combination structures of the present invention; Figure 6a The middle reflective mirror group 3 and the reflective mirror group 4 are staggered and arranged on both sides of the parallel light-transmitting surface, and the angle bisectors of the 90-degree angle of the two reflective mirror groups are parallel. At this time, after the light enters from the light entrance hole / slit 5 on the second reflective part 2 or enters through the light emitted from the previous mirror group, it is emitted to the reflective mirror group 4 after two consecutive reflections from the reflective mirror group 3, and then after two consecutive reflections from the reflective mirror group 4, it is emitted to the first reflective part 1. After multiple reflections, the incident light and the emitted light are on the same plane, which increases the optical path of the light in the system to be measured.

[0042] Figure 6b The middle reflective mirror group 3 and the reflective mirror group 4 are staggered and arranged on both sides of the light-transmitting surface, and the angle bisectors of the 90-degree angle of the two reflective mirror groups are perpendicular to each other. At this time, after the light enters from the light entrance hole / slit 5 on the second reflective part 2 or enters through the light emitted from the previous mirror group, it is emitted to the reflective mirror group 4 after two reflections from the reflective mirror group 3, and then after two reflections from the reflective mirror group 4, it is emitted to the first reflective part 1. In this way, after the sequential reflections of the combined structure, the initial incident light and the final emitted light are on different planes, which increases the optical path of the light in the system to be measured and also changes the direction of the light path.

[0043] Furthermore, the first reflecting mirror group 3 on the first reflecting part 1 and the second reflecting mirror group 4 on the second reflecting part 2 can be arranged at different angles rotated around the corresponding incident light as the axis on a parallel plane relative to the light-transmitting surface of the system to be measured, thereby changing the path of the corresponding emitted light in the system to be measured, so as to further extend the optical path range of the initial incident light of the point light source in the system to be measured.

[0044] Example 7 Figure 7 The figure shows a schematic diagram of a light path of a light emitting component of the present invention for a rectangular light-transmitting surface, including several Figure 6a and 6b The light enters at the position shown in Li and passes through several Figure 6a The combined structure shown in FIG. 1 is reflected sequentially, and the light path is on a plane. Figure 6b The structure shown in the figure makes the light reflected by the reflector surface group 4 perpendicular to the plane where the previous light is located. Figure 7 The path trajectory shown by the middle dotted line moves forward in sequence.

[0045] Furthermore, the reflective mirror group 3 and the reflective mirror group 4 can be rotated and combined at any angle on the parallel plane relative to the light-transmitting surface of the system to be measured with the corresponding incident light as the axis, and the required optical path size can be selected within the limited reflection space, so that the reflected light path is a broken line or arc trajectory. Figure 8 The figure shows a schematic diagram of a spiral light path of the light emitting component of the present invention on a circular light-transmitting surface. This arrangement can comprehensively consider the light-transmitting surface size of the system to be tested and maximize the optical path of the light in the system to be tested during testing.

[0046] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention is described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A light reflection component for extending the optical path of a colorimetric cell, characterized in that: The light reflection component comprises a first reflection part (1) and a second reflection part (2), the first reflection part (1) and the second reflection part (2) are respectively arranged on both sides of the colorimetric cell system to be measured; the first reflection part (1) comprises m groups of first reflection mirror surface groups (3), the second reflection part (2) comprises n groups of second reflection mirror surface groups (4), m is an integer greater than or equal to 1, and n is an integer greater than or equal to 0; the reflection mirror surface groups of the first reflection mirror surface group (3) and the second reflection mirror surface group (4) are both formed by two reflection mirror surfaces intersecting at an angle of 90 degrees, and the 90-degree angle formed by the two reflection mirror surfaces is arranged toward the system to be measured, and the angle bisector plane is perpendicular to the system to be measured; The first reflecting mirror surface group (3) and the second reflecting mirror surface group (4) are arranged in a relatively staggered manner, so that the initial incident light moves forward in sequence under the reflection of the first reflecting mirror surface group (3) and the second reflecting mirror surface group (4); The first reflective mirror group (3) and the second reflective mirror group (4) are arranged in a rotational combination at any angle on a plane parallel to the light-transmitting surface of the system to be measured, with the corresponding incident light as the axis; The light reflection component is provided with a light entry hole / slit (5) and a light exit hole / slit (6), and the light entry hole / slit (5) and the light exit hole / slit (6) are both arranged on the second reflection part (2) or respectively on the second reflection part (2) and the first reflection part (1); The light reflection components are built into the instrument optical path detection system as a set, and the number of sets is an integer greater than or equal to 1; Multiple sets of light reflection components built into the instrument's optical path detection system can be manually or automatically replaced to meet different optical path requirements.

2. The light reflection assembly for extending the optical path of a colorimetric cell according to claim 1, characterized in that: The initial incident light source of the light reflection component is a point light source or a line light source.

3. The light reflection assembly for extending the optical path of a colorimetric cell according to claim 1, characterized in that: The system to be tested may be in liquid or gaseous form.

4. The light reflection assembly for extending the optical path of a colorimetric cell according to claim 1, characterized in that: The system to be measured is optically transparent on both sides of the light path.

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