Composition with synergistic anti-wrinkle effect, application thereof and cosmetics

Abstract

The invention belongs to the technical field of cosmetics, and provides a composition with a synergistic anti-wrinkle effect, application of the composition and cosmetics. The composition provided by the invention comprises tocopherol retinoate and nicotinamide adenine dinucleotide, and the mass part ratio of the tocopherol retinoate to the nicotinamide adenine dinucleotide is 1: (0.02-10). In the composition provided by the invention, the tocopherol retinoate and the nicotinamide adenine dinucleotide have a synergistic anti-wrinkle effect, and the effect of the composition is obviously greater than the sum of the effects of the tocopherol retinoate and the nicotinamide adenine dinucleotide which are independently used in the aspect of promoting the synthesis of the fibroblast collagen I. When the composition with the synergistic anti-wrinkle effect provided by the invention is applied to preparation of cosmetics, the use amount of tocopherol retinoate can be reduced, so that the obvious anti-wrinkle and anti-aging effects are obtained while the irritation is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a composition with synergistic anti-wrinkle effects, application thereof and cosmetics. Background Art

[0002] As people pursue healthier and younger skin, the research and development of anti-aging and anti-wrinkle cosmetics has become an important development direction of the cosmetics industry. Skin aging is a complex physiological process that is affected by a variety of internal and external factors. From the perspective of endogenous factors, with age, the metabolic capacity of cells decreases, the synthesis of collagen and elastin decreases, and the elasticity and firmness of the skin gradually decrease. Exogenous factors such as ultraviolet radiation, environmental pollution, and oxidative stress can also accelerate skin aging and lead to the formation of wrinkles.

[0003] Retinol and its derivatives are classic anti-aging skin care ingredients that have the ability to promote collagen synthesis and promote skin cell proliferation, thereby making the skin resilient and elastic. They also help increase the skin's water retention, help repair lost collagen, and reduce wrinkles and signs of premature aging. The skin irritation of retinol and its derivatives has always been one of the main limiting factors for their use in cosmetics. Tocopheryl Retinoate is an esterification product of vitamin A (retinoic acid) and vitamin E (tocopherol). Although tocopheryl Retinoate is milder than retinol, it still has a certain degree of irritation. Nicotinamide adenine dinucleotide (NAD+) is an important coenzyme in cellular redox reactions and is widely involved in regulating biological processes such as cell growth, differentiation, energy metabolism, and apoptosis. Summary of the invention

[0004] The object of the present invention is to provide a composition with synergistic anti-wrinkle effect, its application in the preparation of cosmetics and cosmetics, which can achieve satisfactory anti-wrinkle and anti-aging effects while reducing the amount of tocopherol retinoic acid ester used and thus reducing its irritation through the synergistic mechanism of tocopherol retinoic acid ester and nicotinamide adenine dinucleotide.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A first aspect of the present invention provides a composition with synergistic anti-wrinkle effect, comprising tocopherol retinoate and nicotinamide adenine dinucleotide, wherein the mass ratio of the tocopherol retinoate to the nicotinamide adenine dinucleotide is 1:(0.02-10).

[0007] Preferably, in the composition with synergistic anti-wrinkle effect provided by the present invention, the mass ratio of the tocopherol retinoic acid ester to the nicotinamide adenine dinucleotide is 1:(0.04-10).

[0008] More preferably, in the composition with synergistic anti-wrinkle effect provided by the present invention, the mass ratio of the tocopherol retinoic acid ester to the nicotinamide adenine dinucleotide is 1:(0.1-2.5).

[0009] The second aspect of the present invention provides use of the composition with synergistic anti-wrinkle effect according to the first aspect of the present invention in the preparation of cosmetics.

[0010] In the above application in preparing cosmetics, the composition with synergistic anti-wrinkle effect provided by the present invention synergistically increases the synthesis of collagen I in fibroblasts.

[0011] The third aspect of the present invention provides a cosmetic comprising the composition with synergistic anti-wrinkle effect according to the first aspect of the present invention.

[0012] Preferably, in the cosmetic provided by the present invention, the mass percentage concentration of tocopherol retinoic acid ester in the cosmetic is 0.001% to 1%.

[0013] Preferably, in the cosmetic provided by the present invention, the mass percentage concentration of nicotinamide adenine dinucleotide in the cosmetic is 0.001% to 5%.

[0014] Compared with the prior art, the present invention provides a composition with synergistic anti-wrinkle effect, which comprises tocopherol retinoate and nicotinamide adenine dinucleotide in a specific mass ratio. In the combination provided by the present invention, tocopherol retinoate and nicotinamide adenine dinucleotide have synergistic anti-wrinkle effect, and in promoting the synthesis of collagen I in fibroblasts, the effect of the composition is significantly greater than the sum of the effects of tocopherol retinoate alone and nicotinamide adenine dinucleotide alone. The composition with synergistic anti-wrinkle effect provided by the present invention is used in the preparation of cosmetics, and significant synergistic anti-wrinkle and anti-aging effects can be obtained while reducing the amount of tocopherol retinoate used, thereby reducing its irritation. DETAILED DESCRIPTION

[0015] In order to make the purpose, technical scheme and advantages of the present invention clearer, the various embodiments of the present invention will be described in detail below. However, it will be appreciated by those skilled in the art that in the various embodiments of the present invention, many technical details are provided in order to enable the reader to better understand the present application. However, even without these technical details and various changes and modifications based on the following embodiments, the technical scheme claimed for protection in the claims of the present application can be implemented.

[0016] According to the first aspect of the present application, an embodiment of the present application provides a composition with synergistic anti-wrinkle effect, which comprises tocopherol retinoate and nicotinamide adenine dinucleotide, wherein the mass ratio of the tocopherol retinoate to the nicotinamide adenine dinucleotide is 1:(0.02-10).

[0017] For example, the mass ratio of tocopheryl retinoate to nicotinamide adenine dinucleotide can be 1:0.02, 1:0.04, 1:0.06, 1:0.08, 1:0.1, 1:0.2, 1:0.5, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, or within a range formed by any two of the above ratios.

[0018] In an embodiment of the present application, the composition with synergistic anti-wrinkle effect comprises tocopherol retinoic acid ester and nicotinamide adenine dinucleotide in a mass ratio of 1: (0.02 to 10). It has been experimentally verified that when tocopherol retinoic acid ester and nicotinamide adenine dinucleotide are compounded in a mass ratio of 1: (0.02 to 10), the obtained composition is significantly more effective in promoting the synthesis of fibroblast collagen I than the sum of the effects of tocopherol retinoic acid ester alone and nicotinamide adenine dinucleotide alone. Therefore, the composition provided in the embodiment of the present application has a synergistic anti-wrinkle effect, and the composition is used in the preparation of cosmetics, and a significant synergistic anti-wrinkle effect can be obtained with a lower concentration of tocopherol retinoic acid ester, thereby effectively reducing the irritation of tocopherol retinoic acid ester to the skin, while obtaining significant anti-wrinkle and anti-aging cosmetics.

[0019] In some embodiments of the present application, the mass ratio of tocopherol retinoate and nicotinamide adenine dinucleotide is 1:(0.04-10); in some embodiments of the present application, the mass ratio of tocopherol retinoate and nicotinamide adenine dinucleotide is 1:(0.1-2.5), thereby achieving more significant synergistic effect in promoting fibroblast collagen I synthesis and anti-wrinkle.

[0020] According to the second aspect of the present application, the embodiments of the present application also provide use of the composition with synergistic anti-wrinkle effect according to the first aspect of the present application in the preparation of cosmetics.

[0021] In the above application in preparing cosmetics, the composition with synergistic anti-wrinkle effect provided by the present invention synergistically increases the synthesis of collagen I in fibroblasts.

[0022] According to the third aspect of the present application, an embodiment of the present application further provides a cosmetic comprising the composition with synergistic anti-wrinkle effect according to the first aspect of the present application.

[0023] In some embodiments of the present application, the mass percentage concentration of tocopherol retinoic acid ester in the cosmetic is 0.001% to 1%; the mass percentage concentration of nicotinamide adenine dinucleotide in the cosmetic is 0.001% to 5%.

[0024] The advantages of the present application are further described below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present application and are not used to limit the scope of the present application.

[0025] Examples 1 to 6, Comparative Examples 1 to 11

[0026] 1. Test system: The cells used in this test are fibroblasts, which are provided by Guangdong Boxi Biotechnology Co., Ltd.

[0027] 2. Main reagents: DMEM culture medium (Gibco), PBS (Solabio), MTT (Sigma), Collagen I ELISA kit (Cusabio).

[0028] 3. Main equipment: CO2 incubator (Thermo, 150I), clean bench (Sujing Antai, SW-CJ-1F), microplate reader (BioTek, Epoch), UVA irradiator (Philips).

[0029] 4. Test method:

[0030] (1) Cell seeding: After the cells are thawed, when the plating rate reaches about 60%, the cells are seeded into a 6-well plate and incubated in an incubator (37°C, 5% CO 2 ) and incubate overnight.

[0031] (2) Prepare samples according to the concentrations in the table below. All samples are dissolved in DMEM culture medium.

[0032] Table 1 Composition and concentration of samples in Examples 1 to 6 and Comparative Examples 1 to 11

[0033]

[0034] (3) Drug administration: When the cell plating rate in the 6-well plate reaches 30% to 50%, the drug administration is carried out in groups, with 3 replicate wells in each group. 2 mL of culture medium is added to each well of the blank control group and the UV control group, and 2 mL of culture medium containing the sample of the corresponding concentration is added to each well of the sample group. After drug administration, the 6-well plate is placed in a CO 2 Incubator (37°C, 5% CO 2 ) for 24 h.

[0035] (4) UVA irradiation: According to the test groups, all groups except the blank control group were irradiated with UVA at a dose of 30 J / cm 2After irradiation, place in CO 2 Incubator (37°C, 5% CO 2 ) and cultured for 24 h.

[0036] (5) ELISA test: After the incubation, the cell culture supernatant was collected in a centrifuge tube, and Collagen I was detected and analyzed according to the operating instructions of the ELISA kit.

[0037] (6) Calculation of Collagen I enhancement rate: enhancement rate (%) = 100% × (average concentration of Collagen I in the sample group - average concentration of Collagen I in the UV control group) / average concentration of Collagen I in the UV control group.

[0038] (7) Collaborative determination:

[0039] a) To illustrate that in the composition with synergistic anti-wrinkle effect provided by the present invention, tocopherol retinoate and nicotinamide adenine dinucleotide have a synergistic effect, rather than just a simple addition of the effects of the two components, the present invention uses the COLBY formula (SR Colby, Weeds, Vol. 15, No. 1, pp. 20-22, 1967) to calculate the theoretical Collagen I enhancement rate of the composition comprising tocopherol retinoate and nicotinamide adenine dinucleotide:

[0040] Etheory = X + Y – [(X·Y) / 100]

[0041] Wherein, X is the rate of collagen I enhancement after administration of a single component, tocopherol retinoic acid ester;

[0042] Y is the rate of collagen I enhancement after administration of a single component, nicotinamide adenine dinucleotide;

[0043] Etheoretical is the theoretical Collagen I enhancement rate after administration of the combination of tocopheryl retinoic acid ester and nicotinamide adenine dinucleotide.

[0044] The E theory of Example 1 is calculated from the data of Comparative Examples 3 and 9, the E theory of Example 2 is calculated from the data of Comparative Examples 4 and 9, the E theory of Example 3 is calculated from the data of Comparative Examples 5 and 9, the E theory of Example 4 is calculated from the data of Comparative Examples 4 and 8, the E theory of Example 5 is calculated from the data of Comparative Examples 5 and 8, and the E theory of Example 6 is calculated from the data of Comparative Examples 6 and 8.

[0045] Comparative Examples 1 to 9 were single-component administrations, and E theory was not calculated.

[0046] The E theory of Comparative Example 10 is calculated from the data of Comparative Examples 2 and 9, the E theory of Comparative Example 11 is calculated from the data of Comparative Examples 2 and 8, and the E theory of Comparative Example 12 is calculated from the data of Comparative Examples 7 and 8.

[0047] b) The experimental results also show the actual Collagen I enhancement rate after administration of tocopherol retinoic acid ester, nicotinamide adenine dinucleotide or the combination thereof of Examples 1-6 and Comparative Examples 10-12, which is recorded as Eactual.

[0048] c) Compare the E theory and E practice of Examples 1-6 and Comparative Examples 10-12, and make a synergistic judgment:

[0049] If Eactual>Etheory, it is efficiency enhancement;

[0050] If Eactual < Etheoretical, it is antagonistic;

[0051] If Eactual = Etheoretical, it is an addition.

[0052] The test results and synergistic judgments of Examples 1 to 6 and Comparative Examples 1 to 12 are shown in Table 2:

[0053] Table 2 Collagen I concentration and synergistic determination of Examples 1 to 6 and Comparative Examples 1 to 12

[0054]

[0055] As can be seen from Table 2, in Example 1, the Etheoretical of 0.00625 μg / mL tocopherol retinoic acid ester and 0.0625 μg / mL nicotinamide adenine dinucleotide is 8.82%, and the Eactual of Example 1 is 19.71%, and Eactual>Etheoretical, which proves that tocopherol retinoic acid ester and nicotinamide adenine dinucleotide have a synergistic effect in promoting the synthesis of collagen I in fibroblasts at a ratio of 1:10.

[0056] Similarly, the actual E of Examples 2 to 6 is greater than the theoretical E, indicating that when the mass ratio of tocopherol retinoate to nicotinamide adenine dinucleotide is in the range of 1: (0.02 to 10), tocopherol retinoate and nicotinamide adenine dinucleotide have a synergistic effect in promoting the synthesis of collagen I in fibroblasts.

[0057] Among them, the actual E of Examples 2 to 4 is not only greater than the theoretical E, but also the ratio of the actual E to the theoretical E is more than 2.5 times, indicating that when the mass ratio of tocopherol retinoic acid ester and nicotinamide adenine dinucleotide is in the range of 1: (0.1 to 2.5), tocopherol retinoic acid ester and nicotinamide adenine dinucleotide have a synergistic effect in promoting the synthesis of collagen I in fibroblasts, which is particularly significant.

[0058] In contrast, Comparative Examples 10 to 12 did not show that Eactual was greater than Etheoretical, indicating that within the ratio range of Comparative Examples 10 to 12, tocopherol retinoic acid ester and nicotinamide adenine dinucleotide did not have a synergistic effect in promoting the synthesis of collagen I in fibroblasts.

[0059] Those skilled in the art will appreciate that the above-mentioned embodiments are specific examples for implementing the present invention, and in actual applications, various changes may be made thereto in form and detail without departing from the spirit and scope of the present invention.

Claims

1. A composition with synergistic anti-wrinkle effect, characterized in that: The invention comprises tocopherol retinoic acid ester and nicotinamide adenine dinucleotide, wherein the mass ratio of the tocopherol retinoic acid ester to the nicotinamide adenine dinucleotide is 1:(0.02-10).

2. The composition with synergistic anti-wrinkle effect according to claim 1, characterized in that: The mass ratio of the tocopherol retinoic acid ester to the nicotinamide adenine dinucleotide is 1:(0.04-10).

3. The composition with synergistic anti-wrinkle effect according to claim 2, characterized in that: The mass ratio of the tocopherol retinoic acid ester to the nicotinamide adenine dinucleotide is 1:(0.1-2.5).

4. Use of the composition with synergistic anti-wrinkle effect according to any one of claims 1 to 3 in the preparation of cosmetics.

5. The use according to claim 4, characterized in that: The composition with synergistic anti-wrinkle effect synergistically increases the synthesis of collagen I in fibroblasts.

6. A cosmetic, characterized in that: The composition having synergistic anti-wrinkle effect comprises any one of claims 1 to 3.

7. The cosmetic according to claim 6, characterized in that: The mass percentage concentration of the tocopherol retinoic acid ester in the cosmetic is 0.001% to 1%.

8. The cosmetic according to claim 6, characterized in that: The mass percentage concentration of the nicotinamide adenine dinucleotide in the cosmetic is 0.001% to 5%.

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