Preparation method of euphausia superba oil with high oxidation stability

Abstract

The invention discloses a preparation method of euphausia superba oil with high oxidation stability, which comprises the following steps: mixing euphausia superba powder with ethanol, extracting the euphausia superba oil under a vacuum condition, removing a solvent, introducing nitrogen into a system of the euphausia superba oil, and performing deoxidation treatment under a vacuum or ultrasonic condition to obtain the euphausia superba oil with high oxidation stability. The effect of improving the oxidation stability of the euphausia superba oil in the preparation process of the euphausia superba oil is achieved, the whole process is easy to operate, the cost is low, and industrial production is facilitated. The oxidation stability of the euphausia superba oil can be obviously improved by combining low-temperature ultrasonic treatment with nitrogen introduction treatment, and the conjugated triene value, peroxide value, thiobarbituric acid value and total oxidation value of the obtained euphausia superba oil are obviously lower than corresponding values of the euphausia superba oil obtained by low-temperature vacuum nitrogen introduction treatment, high-temperature ultrasonic treatment and high-temperature ultrasonic treatment. The oxidation stability of the euphausia superba oil prepared by the method disclosed by the invention is remarkably improved, and the effect of improving the oxidation stability of the euphausia superba oil on the basis of not adding an antioxidant is realized.

Description

Technical Field

[0001] The invention relates to the technical field of Antarctic krill oil processing, and in particular to a method for preparing Antarctic krill oil with high oxidation stability. Background Art

[0002] Antarctic krill (Euphausia superba) is a marine organism that lives in the Antarctic waters. Antarctic krill has a large biological reserve (about 650 million to 1 billion tons), a wide distribution, and high nutritional value. It is an important marine fishery resource with high commercial development value. Antarctic krill oil is a high value-added product in the Antarctic krill deep processing industry chain. It contains rich functional ingredients such as phospholipid-type ω-3 long-chain polyunsaturated fatty acids (ω3LC-PUFAs), astaxanthin and vitamins. Compared with conventional triglyceride-type and ethyl ester-type ω3LC-PUFAs, phospholipid-type ω3LC-PUFAs have higher bioavailability, stronger tissue transport capacity, and better health effects. Studies have shown that Antarctic krill oil has health effects such as anti-obesity, anti-depression, anti-inflammatory, and treatment of cardiovascular disease and Alzheimer's disease. At present, Antarctic krill oil functional products have become the main direction of Antarctic krill commercial development.

[0003] In the production process of Antarctic krill oil, in order to reduce the loss of functional components such as phospholipids and astaxanthin, conventional refining steps such as degumming, deacidification, decolorization, deodorization, and dewaxing are not used, which makes the content of pro-oxidants such as metal ions, moisture, and free fatty acids in Antarctic krill oil much higher than that of ordinary oils. Therefore, during storage, Antarctic krill oil is prone to lipid oxidation, resulting in deterioration of oil quality, manifested as sensory quality deterioration such as darkening of color, increased viscosity, and the production of unpleasant flavors, posing a potential risk to consumers' health. The self-oxidation chain reaction is the most common process in lipid oxidation, which is initiated by free radicals, and oxygen is an important reaction substrate that causes the self-oxidation chain reaction to pass on, and is an important factor leading to lipid oxidation. At present, most strategies to improve oxidative stability rely on the addition of antioxidants to combat free radicals or pro-oxidative metal ions present in lipids. However, consumers have certain concerns about the use of synthetic antioxidants, and natural antioxidants have poor antioxidant effects on Antarctic krill oil. Therefore, it is urgent to establish a method for preparing highly stable Antarctic krill oil based on oxygen removal rather than antioxidant addition. Summary of the invention

[0004] In view of the above problems, the purpose of the present invention is to provide a method for preparing Antarctic krill oil with high oxidation stability, that is, using a deoxygenation method of nitrogen gas combined with ultrasonic treatment or vacuum treatment to improve the oxidation stability of Antarctic krill oil. The Antarctic krill oil prepared by the present invention has high oxidation stability, is suitable for industrial production, and has broad application prospects in the food field.

[0005] To achieve the above object, the present invention first provides a method for preparing Antarctic krill oil with high oxidation stability, comprising the following steps:

[0006] S1. Processing of Antarctic krill powder: steaming Antarctic krill raw materials, hot air drying and then grinding to obtain Antarctic krill powder;

[0007] S2. Extraction of Antarctic krill oil: mixing the Antarctic krill powder prepared in step S1 with an organic solvent, stirring, and filtering to obtain a filtrate;

[0008] S3, removal of organic solvent: the filtrate obtained in step S2 is subjected to high vacuum and low temperature desolventizing treatment to obtain Antarctic krill oil;

[0009] S4, deoxygenation treatment: inert gas is introduced into the Antarctic krill oil obtained in step S3, and ultrasonic treatment or vacuum treatment or vacuum combined with ultrasonic treatment is performed to obtain the Antarctic krill oil with high stability from which oxygen is removed.

[0010] In one embodiment of the present invention, the cooking temperature in step S1 is 80-100° C.; the cooking time is 3-5 min.

[0011] In one embodiment of the present invention, the hot air drying parameters in step S1 are: hot air drying at 80° C. for 2.2 hours, and then maintaining at 50° C. for 0.3-1 hour.

[0012] In one embodiment of the present invention, the organic solvent in step S2 includes ethanol, and the ethanol is preferably food-grade ethanol, 95% ethanol or anhydrous ethanol.

[0013] In one embodiment of the present invention, the mass volume ratio of Antarctic krill powder to organic solvent in step S2 is 1 kg: (5-10) L.

[0014] In one embodiment of the present invention, the stirring in step S2 is carried out at 20-25° C. and a rotation speed of 500-700 rpm for 2-3 hours; the porosity of the filter membrane used in the filtration is 0.45 μm.

[0015] In one embodiment of the present invention, the desolvation treatment in step S3 is to desolvate for 0.5 to 1 h under the conditions of a vacuum degree of 10000±200 Pa and a temperature of 30 to 35°C.

[0016] In one embodiment of the present invention, the inert gas in step S4 includes nitrogen and / or argon.

[0017] In one embodiment of the present invention, the purity of the inert gas is above 99.9%, and the flow rate of the inert gas is 0.5 to 1 L / min / kg Antarctic krill oil.

[0018] In one embodiment of the present invention, the power of the ultrasonic treatment in step S4 is 160-200 W, the time is 0.5-1 h, and the temperature is 20-35° C., preferably 20-30° C.

[0019] In one embodiment of the present invention, the vacuum degree of the vacuum treatment in step S4 is 10000±200 Pa, the vacuum time is 0.5-1h, and the vacuum treatment temperature is 20-35°C, preferably 20-30°C.

[0020] In one embodiment of the present invention, the temperature of the ultrasonic combined vacuum treatment in step S4 is 20-35°C, the treatment time is 0.5-1h, the vacuum degree is 10000±200Pa, and the ultrasonic treatment power is 160-200W.

[0021] The present invention also provides Antarctic krill oil with high oxidation stability prepared according to the method.

[0022] The present invention also provides application of the Antarctic krill oil with high oxidation stability in food.

[0023] Beneficial effects:

[0024] 1. Antarctic krill powder is mixed with ethanol, and Antarctic krill oil is extracted under vacuum conditions. After desolventization, nitrogen is introduced into the Antarctic krill oil system, and deoxygenation is performed under vacuum or ultrasonic conditions, thereby achieving the effect of improving the oxidative stability of Antarctic krill oil during its preparation. The whole process is simple to operate, low in cost, and is conducive to industrial production.

[0025] 2. Low-temperature (20°C) ultrasonic nitrogen treatment can significantly improve the oxidative stability of Antarctic krill oil. The conjugated triene value, peroxide value, thiobarbituric acid value and total oxidation value of the obtained Antarctic krill oil are significantly lower than the corresponding values ​​of Antarctic krill oil obtained by low-temperature vacuum nitrogen treatment, high-temperature ultrasonic nitrogen treatment and high-temperature vacuum nitrogen treatment.

[0026] 3. The oxidative stability of Antarctic krill oil treated by low-temperature (20°C) vacuum nitrogen combined with ultrasound is significantly improved, achieving the effect of improving the oxidative stability of Antarctic krill oil without adding antioxidants. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 The results are the changes in the conjugated triene values ​​of the Antarctic krill oil prepared in Examples 1 to 4 and Comparative Examples 1 to 4 during accelerated storage at 60° C. The different letters in the data in the figure indicate that the differences between the samples are significant, P<0.05.

[0028] Figure 2The results are the changes in the peroxide value of the Antarctic krill oil prepared in Examples 1 to 4 and Comparative Examples 1 to 4 during accelerated storage at 60° C. The different letters in the data in the figure indicate that the differences between the samples are significant, P<0.05.

[0029] Figure 3 The results are the changes in the thiobarbituric acid value of the Antarctic krill oil prepared in Examples 1 to 4 and Comparative Examples 1 to 4 during accelerated storage at 60° C. The different letters in the data in the figure indicate that the difference between the samples is significant, P<0.05.

[0030] Figure 4 The graph is the change result of the total oxidation value of the Antarctic krill oil prepared in Examples 1 to 4 and Comparative Examples 1 to 4 during accelerated storage at 60° C. Different letters in the data in the graph indicate that the difference between the samples is significant, P<0.05. DETAILED DESCRIPTION

[0031] In order to further understand the present invention, the preparation method of the Antarctic krill oil with high oxidation stability provided by the present invention is described below in conjunction with examples, and the protection scope of the present invention is not limited by the following examples.

[0032] Testing process

[0033] 1. Conjugated triene value:

[0034] Dissolve Antarctic krill oil (0.01 g) in isooctane and dilute to 10 mL. Use isooctane as a blank and measure the absorbance at 268 nm using a spectrophotometer. Calculate the conjugated triene value according to the formula:

[0035] CT=A / C×P (Formula 1)

[0036] Wherein, A is the absorbance of the sample at 268 nm; C is the final dilution concentration of the sample (g / 100 mL); and P is the length of the measuring dish (cm).

[0037] 2. Peroxide value:

[0038] Fully dissolve Antarctic krill oil (0.01 g) in a mixture of 1.5 mL of dichloromethane and 95% ethanol (volume ratio 3:2). Then, add 5 mM ammonium ferrous sulfate hexahydrate (100 μL), 1 M methanol-xylenol orange tetrasodium salt (200 μL) and 0.25 M methanol-sulfuric acid (200 μL) and mix well. After standing at room temperature and in the dark for 30 minutes, add boiled and cooled pure water (1 mL) to the reaction system. Then centrifuge at 4000 × g for 5 minutes. Take the upper layer of the mixture (200 μL) and measure the absorbance (A) at 560 nm. 560). Draw the standard curve of cumene hydroperoxide and calculate the peroxide value (mg / g oil).

[0039] POV (mg / g oil) = (A-0.079) / 0.0135×0.15 (Formula 2)

[0040] Where A is the absorbance of the sample at 560 nm.

[0041] 3. Thiobarbituric acid value:

[0042] Antarctic krill oil (0.05 g) was uniformly mixed with the mixed solution (2.5 mL) to obtain a mixture, wherein the mixed solution included distilled water (196 mL), concentrated hydrochloric acid solution (4.17 mL), thiobarbituric acid (0.75 g) and trichloroacetic acid (30 g). The mixture was heated in a boiling water bath for 10 min, cooled and centrifuged at 3000×g for 10 min, and the upper layer of the mixture (200 μL) was taken and the absorbance (A) was measured at 532 nm. 532 ). The standard curve of 1,1,3,3-tetraethoxypropane was plotted and the thiobarbituric acid value (mg MDA / kg oil) was calculated.

[0043] TBARS(mg MDA / kg oil)=(A-0.0264) / 0.7302 (Formula 3)

[0044] Where A is the absorbance of the sample at 532 nm.

[0045] 4. Total oxidation value:

[0046] The total oxidation value is calculated according to the formula:

[0047] TOTOX=2POV+TBARS (Formula 4)

[0048] Wherein, POV is the peroxide value of the sample; TBARS is the thiobarbituric acid value of the sample.

[0049] Embodiment 1:

[0050] A method for preparing Antarctic krill oil with high oxidation stability comprises the following steps:

[0051] S1. Boil Antarctic krill raw materials (100° C. for 3 min) and chop them, dry them with hot air at 80° C. for 2.2 h, and then dry them with hot air at 50° C. for 0.5 h before grinding them into powder to obtain Antarctic krill powder.

[0052] S2. Take the Antarctic krill powder obtained in S1 and mix it with anhydrous ethanol in a ratio of 1:5 (mass / volume, kg / L), stir it at 25° C. and 500 rpm for 3 h, and filter it to obtain a filtrate.

[0053] S3. The filtrate obtained in S2 is deethanolated for 1 hour at a vacuum degree of 10000 Pa and a temperature of 35° C. to obtain Antarctic krill oil.

[0054] S4. The Antarctic krill oil obtained in S3 is placed under a vacuum of 10000 Pa, and ultrasonically treated at an ultrasonic power of 160 W and a temperature of 20° C. for 0.5 h, while nitrogen (purity 99.9%) is introduced at a flow rate of 0.5 L / min / kg of Antarctic krill oil to obtain Antarctic krill oil with high oxidation stability. The product is named UVT-20° C.

[0055] Embodiment 2:

[0056] The difference between Example 2 and Example 1 is that step S4 is different.

[0057] A method for preparing Antarctic krill oil with high oxidation stability comprises the following steps:

[0058] S1. Boil Antarctic krill raw materials (100° C. for 3 min) and chop them, dry them with hot air at 80° C. for 2.2 h, and then dry them with hot air at 50° C. for 0.5 h before grinding them into powder to obtain Antarctic krill powder.

[0059] S2. Take the Antarctic krill powder obtained in S1 and mix it with anhydrous ethanol in a ratio of 1:5 (mass / volume, kg / L), stir it at 25° C. and 500 rpm for 3 h, and filter it to obtain a filtrate.

[0060] S3. The filtrate obtained in S2 is deethanolated for 1 hour at a vacuum degree of 10000 Pa and a temperature of 35° C. to obtain Antarctic krill oil.

[0061] S4. The Antarctic krill oil obtained in S3 is placed under a vacuum of 10000 Pa, and ultrasonically treated at an ultrasonic power of 160 W and a temperature of 35° C. for 0.5 h, while nitrogen (purity 99.9%) is introduced at a flow rate of 0.5 L / min / kg of Antarctic krill oil to obtain Antarctic krill oil with high oxidation stability. The product is named UVT-35° C.

[0062] Example 3

[0063] The difference between Example 3 and Example 1 is that step S4 is different.

[0064] A method for preparing Antarctic krill oil with high oxidation stability comprises the following steps:

[0065] S1. Boil Antarctic krill raw materials (100° C. for 3 min) and chop them, dry them with hot air at 80° C. for 2.2 h, and then dry them with hot air at 50° C. for 0.5 h before grinding them into powder to obtain Antarctic krill powder.

[0066] S2. Take the Antarctic krill powder obtained in S1 and mix it with anhydrous ethanol in a ratio of 1:5 (mass / volume, kg / L), stir it at 25° C. and 500 rpm for 3 h, and filter it to obtain a filtrate.

[0067] S3. The filtrate obtained in S2 is deethanolated for 1 hour at a vacuum degree of 10000 Pa and a temperature of 35° C. to obtain Antarctic krill oil.

[0068] S4. The Antarctic krill oil obtained in S3 was ultrasonically treated at an ultrasonic power of 160 W and a temperature of 20° C. for 0.5 h, and nitrogen (purity 99.9%) was introduced at a flow rate of 0.5 L / min / kg of Antarctic krill oil to obtain Antarctic krill oil with high oxidation stability. The product was named UT-20° C.

[0069] Example 4

[0070] The difference between Example 4 and Example 1 is that step S4 is different.

[0071] A method for preparing Antarctic krill oil with high oxidation stability comprises the following steps:

[0072] S1. Boil Antarctic krill raw materials (100° C.; 3 min) and chop them, dry them with hot air at 80° C. for 2.2 h, and then dry them with hot air at 50° C. for 0.5 h before grinding them into powder to obtain Antarctic krill powder.

[0073] S2. Take the Antarctic krill powder obtained in S1 and mix it with anhydrous ethanol in a ratio of 1:5 (mass / volume, kg / L), stir it at 25° C. and 500 rpm for 3 h, and filter it to obtain a filtrate.

[0074] S3. The filtrate obtained in S2 is deethanolated for 1 hour at a vacuum degree of 10000 Pa and a temperature of 35° C. to obtain Antarctic krill oil.

[0075] S4. The Antarctic krill oil obtained in S3 is placed under a vacuum of 10000 Pa and subjected to vacuum treatment at a temperature of 20°C for 0.5 h. Nitrogen (purity 99.9%) is introduced at a flow rate of 0.5 L / min / kg of Antarctic krill oil to obtain Antarctic krill oil with high oxidation stability. The product is named VT-20°C.

[0076] Comparative Example 1

[0077] The difference between Comparative Example 1 and Example 1 is that step S4 is omitted.

[0078] S1. Boil Antarctic krill raw materials (100° C.; 3 min) and chop them, dry them with hot air at 80° C. for 2.2 h, and then dry them with hot air at 50° C. for 0.5 h before grinding them into powder to obtain Antarctic krill powder.

[0079] S2. Take the Antarctic krill powder obtained in S1 and mix it with anhydrous ethanol in a ratio of 1:5 (mass / volume, kg / L), stir it at 25° C. and 500 rpm for 3 h, and filter it to obtain a filtrate.

[0080] S3. The filtrate obtained in S2 is deethanolated for 1 hour at a vacuum degree of 10000 Pa and a temperature of 35° C. to obtain Antarctic krill oil, which is named Con.

[0081] Comparative Example 2

[0082] The difference between Comparative Example 2 and Example 1 is that step S4 is different.

[0083] S1. Boil Antarctic krill raw materials (100° C.; 3 min) and chop them, dry them with hot air at 80° C. for 2.2 h, and then dry them with hot air at 50° C. for 0.5 h before grinding them into powder to obtain Antarctic krill powder.

[0084] S2. Take the Antarctic krill powder obtained in S1 and mix it with ethanol in a ratio of 1:5 (mass / volume, kg / L), stir it at 25° C. and 500 rpm for 3 h, and filter it to obtain a filtrate.

[0085] S3. The filtrate obtained in S2 is deethanolated for 1 hour at a vacuum degree of 10000 Pa and a temperature of 35° C. to obtain Antarctic krill oil.

[0086] S4. The Antarctic krill oil obtained in S3 is placed under normal pressure and temperature of 20°C, and nitrogen (purity 99.9%) is introduced at a flow rate of 0.5L / min / kg of Antarctic krill oil to obtain treated Antarctic krill oil, which is named Con-N 2 .

[0087] Comparative Example 3

[0088] The difference between Comparative Example 3 and Example 1 is that step S4 is different.

[0089] A method for preparing Antarctic krill oil with high oxidation stability comprises the following steps:

[0090] S1. Boil Antarctic krill raw materials (100° C.; 3 min) and chop them, dry them with hot air at 80° C. for 2.2 h, and then dry them with hot air at 50° C. for 0.5 h before grinding them into powder to obtain Antarctic krill powder.

[0091] S2. Take the Antarctic krill powder obtained in S1 and mix it with ethanol in a ratio of 1:5 (mass / volume, kg / L), stir it at 25° C. and 500 rpm for 3 h, and filter it to obtain a filtrate.

[0092] S3. The filtrate obtained in S2 is deethanolated for 1 hour at a vacuum degree of 10000 Pa and a temperature of 35° C. to obtain Antarctic krill oil.

[0093] S4. The Antarctic krill oil obtained in S3 was ultrasonically treated at an ultrasonic power of 160 W and a temperature of 35° C. for 0.5 h, and nitrogen (purity 99.9%) was introduced at a flow rate of 0.5 L / min / kg of Antarctic krill oil to obtain Antarctic krill oil with high oxidation stability. The product was named UT-35° C.

[0094] Comparative Example 4

[0095] The difference between Comparative Example 4 and Example 1 is that step S4 is different.

[0096] A method for preparing Antarctic krill oil with high oxidation stability comprises the following steps:

[0097] S1. Boil Antarctic krill raw materials (100° C.; 3 min) and chop them, dry them with hot air at 80° C. for 2.2 h, and then dry them with hot air at 50° C. for 0.5 h before grinding them into powder to obtain Antarctic krill powder.

[0098] S2. Take the Antarctic krill powder obtained in S1 and mix it with ethanol in a ratio of 1:5 (mass / volume, kg / L), stir it at 25° C. and 500 rpm for 3 h, and filter it to obtain a filtrate.

[0099] S3. The filtrate obtained in S2 is deethanolated for 1 hour at a vacuum degree of 10000 Pa and a temperature of 35° C. to obtain Antarctic krill oil.

[0100] S4. The Antarctic krill oil obtained in S3 is placed under a vacuum of 10000 Pa and subjected to vacuum treatment at 35°C for 0.5 h. Nitrogen (purity 99.9%) is introduced at a flow rate of 0.5 L / min / kg of Antarctic krill oil to obtain Antarctic krill oil with high oxidation stability. The product is named VT-35°C.

[0101] The Antarctic krill oils prepared in Examples 1 to 4 and Comparative Examples 1 to 4 were placed in a constant temperature oven (60° C.), and samples were taken every 2 days to measure the conjugated triene value, peroxide value, thiobarbituric acid value and total oxidation value of the Antarctic krill oil samples. The results are as follows: Figures 1 to 4As shown. As the storage time of Antarctic krill oil increases, the four oxidation indicators all show a gradual upward trend, indicating that the degree of oxidation of Antarctic krill oil gradually increases. After 4 days of storage, the conjugated triene value, peroxide value, thiobarbituric acid value and total oxidation value of the Antarctic krill oil samples (Comparative Examples 1-2) that were not treated with ultrasound and vacuum methods were significantly higher than those of the other six groups of treated Antarctic krill oil (Examples 1-4, Comparative Examples 3-4). The degree of oxidation of the Antarctic krill oil sample (Example 1) treated with low-temperature vacuum nitrogen combined with ultrasound was significantly lower than that of the Antarctic krill oil samples treated with single low-temperature and heated ultrasonic nitrogen method, single low-temperature and heated vacuum nitrogen method, and heated vacuum nitrogen combined with ultrasound method (Examples 2-4, Comparative Examples 3-4). It can be seen that low-temperature vacuum nitrogen combined with ultrasound treatment has the best effect on improving the oxidative stability of Antarctic krill oil.

[0102] The embodiments provided above are not intended to limit the scope of the present invention, and the steps described are not intended to limit the execution order thereof. Those skilled in the art may make obvious improvements to the present invention in combination with existing common knowledge, which also fall within the scope of protection defined by the claims of the present invention.

Claims

1. A method for preparing Antarctic krill oil with high oxidation stability, characterized in that: The following steps are involved: S1. Processing of Antarctic krill powder: steaming Antarctic krill raw materials, hot air drying and then grinding to obtain Antarctic krill powder; S2. Extraction of Antarctic krill oil: mixing the Antarctic krill powder prepared in step S1 with an organic solvent, stirring, and filtering to obtain a filtrate; S3, removal of organic solvent: the filtrate obtained in step S2 is subjected to high vacuum and low temperature desolventizing treatment to obtain Antarctic krill oil; S4, deoxygenation treatment: inert gas is introduced into the Antarctic krill oil obtained in step S3, and ultrasonic treatment or vacuum treatment or vacuum combined with ultrasonic treatment is performed to obtain the Antarctic krill oil with high stability from which oxygen is removed.

2. The preparation method according to claim 1, characterized in that: The cooking temperature in step S1 is 80-100° C.; the cooking time is 3-5 minutes; the hot air drying parameters are: hot air drying at 80° C. for 2.2 hours, and then maintaining at 50° C. for 0.3-1 hour.

3. The preparation method according to claim 1, characterized in that: The organic solvent in step S2 includes ethanol, and the ethanol is preferably food-grade ethanol, 95% ethanol or anhydrous ethanol. The mass volume ratio of the Antarctic krill powder to the organic solvent is 1kg:5-10L. The stirring is maintained for 2-3h at 20-25°C and a rotation speed of 500-700rpm; the porosity of the filter membrane used for the filtration is 0.45μm.

4. The preparation method according to claim 1, characterized in that: The desolvation treatment in step S3 is carried out under the conditions of vacuum degree of 10000±200 Pa and temperature of 30-35° C. for 0.5-1 h.

5. The preparation method according to claim 1, characterized in that: The inert gas in step S4 includes nitrogen and / or argon, the purity of the inert gas is above 99.9%, and the flow rate of the inert gas is 0.5-1 L / min / kg Antarctic krill oil.

6. The preparation method according to claim 1, characterized in that: The power of the ultrasonic treatment in step S4 is 160-200 W, the time is 0.5-1 h, and the temperature is 20-35° C.

7. The preparation method according to claim 1, characterized in that: The vacuum degree of the vacuum treatment in step S4 is 10000±200 Pa, the vacuum time is 0.5-1h, and the vacuum treatment temperature is 20-35°C.

8. The preparation method according to claim 1, characterized in that: The temperature of the ultrasonic combined vacuum treatment in step S4 is 20-35° C., the treatment time is 0.5-1 h, the vacuum degree is 10000±200 Pa, and the ultrasonic treatment power is 160-200 W.

9. Antarctic krill oil with high oxidation stability prepared according to the preparation method according to any one of claims 1 to 8.

10. Use of the Antarctic krill oil with high oxidation stability according to claim 9 in food.

Images