Kodakella ohmeri, bacterial preparation, water purification system and application of Kodakella ohmeri

Abstract

The invention belongs to the technical field of biological water purification, and particularly relates to Kodaea ohmeri, a bacterial preparation, a water purification system and application. Aiming at the characteristic of low COD (Chemical Oxygen Demand) concentration of a polluted water area, a novel microbial agent is developed, meanwhile, a carrier is used for immobilization, and a novel'microorganism-carrier-plant 'coupled water purification system is constructed by combining the advantages of plant water purification. The water purification system can remove nitrogen, phosphorus and organic matters in sewage at the same time. In the water purification system provided by the invention, the lophatherum gracile has extremely high medicinal value and nutritional value, and can be collected and utilized after water purification, so that the economic value is high, and the maintenance cost is low.

Description

Technical Field

[0001] The invention belongs to the technical field of biological water purification, and specifically relates to an Omokoda yeast, a bacterial preparation, a water purification system and applications. Background Art

[0002] Water pollution has gradually become one of the main problems in global environmental protection. The deterioration of river water quality not only affects the health of the ecosystem, but also poses severe challenges to human life, economic development and social stability.

[0003] At present, river water purification technologies can be divided into chemical purification, physical purification and biological purification. Among them, chemical purification has the risk of introducing pollutants into the water body and causing secondary pollution; physical purification has high costs and is easy to damage the water ecosystem; biological purification has the advantages of being environmentally friendly, low cost, simple technology, and easy maintenance, and is currently a hot topic in the field of water environment restoration and water purification research.

[0004] Adding microorganisms is a commonly used biological purification method. However, since the COD concentration in river water is usually low (<50mg / L) and the main components of organic matter are difficult-to-degrade substances such as humic acid, conventional microbial agents have problems such as slow growth, poor degradation effect, and easy loss of functional bacteria; and microbial agents cannot effectively remove phosphorus from river water. Using landscape plants (such as aquatic plants and wetland plants) to purify river water usually has a good removal effect on nitrogen and phosphorus, but there are generally problems such as high plant maintenance costs and poor water purification effects. In addition, plants cannot remove organic matter in the water, and the technology urgently needs to be optimized and improved.

[0005] Lophatherum gracile Brongn. is a perennial herbaceous plant of the genus Lophatherum in the Poaceae family. It has vigorous growth and strong tillering ability. The aboveground part of the plant can be used as medicine. In addition, Lophatherum gracile is rich in nutrients such as polysaccharides, phenols, amino acids, and minerals. It is the raw material for making a variety of nutritious foods and even medicines, and has extremely high economic value. However, there is no report on the water purification ability of Lophatherum gracile, and it is not clear whether it can cooperate with microorganisms to purify water.

[0006] Therefore, it is of great practical significance to study the water purification effect of light bamboo leaves and its synergistic water purification effect with microorganisms, develop efficient microbial agents, and build a more efficient water purification coupling system, which will help to ensure water purification effects, improve economic benefits, and reduce maintenance costs. Summary of the invention

[0007] The purpose of the present invention is to provide an Omokoda yeast, a bacterial preparation, a water purification system and applications.

[0008] To achieve the above-mentioned purpose of the invention, the technical solution adopted by the present invention is: a strain of Kodamaeaohmeri, and the preservation number of the Kodamaeaohmeri is: CGMCCNO.33277.

[0009] Correspondingly, a bacterial preparation prepared using the Omokoda yeast; or; a bacterial preparation containing the Omokoda yeast.

[0010] Correspondingly, a bacterial preparation comprises Pseudomonas, Enterobacter, Trichosporon and Omokoda yeast, and the preservation number of the Omokoda yeast is: CGMCC NO.33277.

[0011] Preferably, the Pseudomonas is Pseudomonas pseudoalcaligenes; and / or; the Enterobacter is Enterobacter cloacae; and / or; the Trichosporon is Trichosporon dermestii.

[0012] Correspondingly, a water purification system comprises the Omokoda yeast, or comprises the bacterial preparation.

[0013] Preferably, the water purification system also includes plants with water purification capabilities.

[0014] Preferably, the plants having water purification ability include bamboo leaves.

[0015] Preferably, the water purification system also includes a microbial carrier.

[0016] Correspondingly, the preparation method of the water purification system is to put the Omokoda yeast, or the bacterial preparation, or the Omokoda yeast / bacterial preparation and a carrier into a matrix for planting the plants, then use the matrix to plant the plants, plant the plants in the sewage to be treated, or use the sewage to be treated to irrigate the plants.

[0017] Correspondingly, a method for treating sewage using the Omokoda yeast, or the bacterial preparation, or the water purification system.

[0018] The present invention has the following beneficial effects:

[0019] In order to improve the biological purification effect of river water and achieve the purpose of simultaneously removing nitrogen, phosphorus and COD in the water body, the present invention has developed a new microbial agent based on the characteristics of low COD concentration in polluted waters, and fixed the microorganisms with carriers. Combined with the advantages of plant water purification, a new "microorganism-carrier-plant" coupled water purification system is constructed.

[0020] The water purification system has a total nitrogen removal efficiency of more than 85% and ammonia nitrogen removal rate of more than 75%, and can purify the ammonia nitrogen level from surface water Class III to surface water Class II. The presence of the carrier in the system makes it easier for the roots of the light bamboo leaves to attach and grow, thereby strengthening the absorption of total nitrogen. At the same time, the carrier is preferably a slow-release carbon source, which is conducive to the enrichment and growth of denitrifying bacteria, so as to further improve the nitrogen removal capacity.

[0021] The water purification system has a total phosphorus removal rate of about 90%, and can purify the total phosphorus level from Class V surface water to Class II surface water. The carrier and microorganisms can strengthen the root growth of the light bamboo leaves, thereby enhancing the plant's absorption and utilization of total phosphorus and improving the system's ability to remove phosphorus.

[0022] The water purification system has a COD removal rate of about 70%, and can purify COD from surface water class V to surface water class II. This is mainly because the microbial agent and the light bamboo leaves work together to effectively degrade low-concentration COD, and the carrier can enrich and promote the growth of functional bacteria in the agent, thereby strengthening the system's COD removal.

[0023] In addition, in the water purification system provided by the present invention, the light bamboo leaves have extremely high medicinal value and nutritional value, and can be collected and utilized after water purification, with high economic value and low maintenance cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 Schematic diagram of sewage treatment in a water purification system provided by the present invention;

[0025] Figure 2 This is a schematic diagram of the COD and ammonia nitrogen degradation levels of each group of microorganisms preliminarily screened in the present invention;

[0026] Figure 3 This is a schematic diagram of the degradation of COD and ammonia nitrogen levels by the combined microorganisms screened in the present invention;

[0027] Figure 4 The colony morphology of the Omokoda yeast provided by the present invention is shown. DETAILED DESCRIPTION

[0028] The present invention provides a new strain of Kodamaea ohmeri, whose ITS sequence is shown in SEQ ID NO: 1. The Kodamaea ohmeri was deposited in the China General Microbiological Culture Collection Center (CGMCC) on January 2, 2025, with a deposit number of CGMCC NO.33277.

[0029] Based on the Omokoda yeast, the present invention also provides a bacterial preparation, including Pseudomonas, Enterobacter, Trichosporon and the Omokoda yeast. Preferably, in terms of the ratio of live bacteria, Pseudomonas: Enterobacter: Omokoda yeast: Trichosporon = (1-3): (1-3): (1-3): (1-3); more preferably: Pseudomonas: Enterobacter: Omokoda yeast: Trichosporon = 2:2:3:1. The Pseudomonas is preferably Pseudomonas pseudoalcaligenes, the Enterobacter is preferably Enterobacter cloacae, and the Trichosporon is preferably Trichosporon dermestii.

[0030] The bacterial preparation has good denitrification and dephosphorization effects, can reduce sewage COD, and can be directly used for sewage purification. In order to improve the water purification effect, based on the bacterial preparation, the present invention also provides a water purifier, which couples the bacterial preparation, light bamboo leaves and a carrier to form a "light bamboo leaves-microorganism-carrier" coupled water purification system. The water purification system includes light bamboo leaves, and the "microorganism-carrier" is put into the planting matrix of light bamboo leaves, which helps the light bamboo leaves grow while facilitating the rapid colonization of microorganisms and occupying dominant ecological niches, thereby improving the water purification effect.

[0031] The microorganism in the system can use the Omokoda yeast alone or the bacterial preparation.

[0032] The carrier is a substance that facilitates the rapid attachment and colonization of microorganisms and has no negative impact on the growth of microorganisms and light bamboo leaves, such as expanded clay, activated carbon, etc.; the preferred scheme is that the carrier has a certain promoting effect on the growth of microorganisms and / or the growth of light bamboo leaves, and has a certain synergistic effect on the removal of nitrogen and phosphorus in sewage.

[0033] An optional embodiment of the carrier is the carrier in the inventor's prior patent CN117602732B: crush the biochar and zeolite into 60-120 mesh particles, melt PBS or PCL at 120°C or 65°C, add 28%-40% (w / w) biochar and zeolite particles, 0.1%-0.2% (w / w) ZnSO4, 0.4%-0.8% (w / w) Fe2(SO4)3 and 0.05%-0.09% (w / w) CuO. Stir for 20 minutes and extrude to obtain particles 1 with a diameter of 2-3 mm. Add 2g of sodium alginate, 0.0002g-0.002g of L-aspartic acid, biotin and cytokinin to 100mL of pure water in a 60°C water bath to dissolve and obtain a hydrogel solution. Place particle 1 in the hydrogel solution and stir to fully wrap the colloidal solution on the surface of particle 1 to obtain particle 2. The particles 2 were taken out and placed in a 2% (w / w) CaCl2 curing solution and cured at 4°C for 24 hours to obtain the desired carrier.

[0034] The present invention also provides a method for preparing the "light bamboo leaf-microorganism-carrier" coupled water purification system, which specifically comprises the following steps:

[0035] 1. Prepare microorganisms (Omokoda yeast seed solution or bacterial preparation) and commercially purchase or prepare carriers.

[0036] 2. The carrier and the microorganism are mixed in a volume ratio of 1:2 to 2:1 (preferably 1:1), and cultured with shaking at 25 to 35°C for 36 to 60 hours to allow the carrier to fully enrich the microorganisms and form a "microorganism-carrier" coupling matrix.

[0037] 3. Add 0.05% to 0.5% (preferably 0.1%) of the "microorganism-carrier" coupling matrix to the light bamboo leaf planting matrix, stir and mix thoroughly, and then plant light bamboo leaves. The preferred planting density is 20 to 25 plants / m 2 , planting depth 10 ~ 15cm, coupling matrix application amount 0.1 ~ 0.15m 3 / m 3 ; Form a "light bamboo leaf-microorganism-carrier" coupled water purification system. Figure 1 As shown, the sewage to be treated flows into the system from one end, is treated by the water purification system, and then flows out from the other end; the whole forms an artificial wetland or ecological interception zone or other similar ecological treatment forms. The planting matrix of the light bamboo leaf can be selected from the planting matrix known in the industry to be able to grow light bamboo leaves without introducing new pollutants, preferably in a mass ratio of 1:1:1:1 mixed with local soil: zeolite: ceramsite: volcanic rock.

[0038] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Unless otherwise specified, the technical means used in the embodiments are conventional means well known to those skilled in the art. The data obtained are all average values ​​obtained after at least 3 repetitions, and all the data obtained in each repetition are valid data.

[0039] Example 1: Screening and identification of microorganisms and bacterial preparations

[0040] 1. Screening of oligotrophic indigenous degrading bacteria

[0041] The slightly polluted river water referred to in the examples refers to water bodies whose water quality indicators exceed the Class III surface water standard (GB3838-2002), including Class IV, V, and inferior Class V surface water bodies. -1 , 10 -2 , 10 -3 , 10 -4Four gradients). 0.08 mL of each concentration gradient dilution was evenly spread on three solid culture media, LB, PDB and Gao's No. 1, and cultured at 30°C. The colonies with obvious appearance differences were picked and streaked on the selective culture medium with slightly polluted river water as the only carbon source and energy source (after adding 2% agar to the slightly polluted river water, sterilized at 121°C for 15 minutes), and cultured again at 30°C. The streaking was repeated three times for purification and separation, and the oligotrophic COD degrading bacteria were screened out.

[0042] Slightly polluted river water was used for aeration enrichment culture of autotrophic and heterotrophic nitrifying bacteria. Among them:

[0043] Autotrophic nitrifying bacteria culture medium: ammonium sulfate 0.5 g / L, sodium bicarbonate 1 g / L, dipotassium hydrogen phosphate 0.4 g / L, potassium dihydrogen phosphate 0.2 g / L, magnesium sulfate heptahydrate 0.5 g / L.

[0044] Heterotrophic nitrifying bacteria culture medium: ammonium sulfate 0.5 g / L, sodium acetate 2 g / L, dipotassium hydrogen phosphate 0.4 g / L, potassium dihydrogen phosphate 0.2 g / L, magnesium sulfate heptahydrate 0.5 g / L.

[0045] 20% (v / v) of the slightly polluted river water was inoculated into the autotrophic nitrifying bacteria culture medium and the heterotrophic nitrifying bacteria culture medium, respectively, and cultured at 30°C and 120 rpm / min for 10 days. 0 , 10 -1 , 10 -2 , 10 -3 , 10 -4 Five gradients), and 0.1 mL of each concentration gradient dilution was evenly applied on the Weiss salt solid medium. The growing plate was subjected to Griess reagent color reaction, and the red colonies were picked and streaked multiple times to separate and purify oligotrophic autotrophic nitrifying bacteria and heterotrophic nitrifying bacteria.

[0046] A total of 15 oligotrophic strains with degradation effects were isolated and screened, including 10 COD-degrading bacteria, including X-3, X-5, X-8, X-10, F-1, F-2, F-4, F-5, Z-2, and Z-5, and 5 ammonia-nitrogen-degrading bacteria (autotrophic nitrifying bacteria and heterotrophic nitrifying bacteria), including H-1, H-3, H-7, Y-9, and Y-17 (H codenamed autotrophic nitrifying bacteria, and Y codenamed heterotrophic nitrifying bacteria).

[0047] 2. Construction of oligotrophic indigenous microbial agents

[0048] The microorganisms screened in step 1 were grouped into groups: Group X included X-3, X-5, X-8, and X-10; Group F included F-1, F-2, F-4, and F-5; Group Z included Z-2 and Z-5; Group Q included all 10 strains of COD-degrading strains; Group H included H-1, H-3, and H-7; Group Y included Y-9 and Y-17; and Group B included all 5 strains of ammonia-nitrogen-degrading bacteria. Each microorganism was prepared as OD 600 =1 seed solution, and mix equal volumes of each microbial seed solution in groups.

[0049] The COD and ammonia nitrogen degradation performance test was carried out on each group. The determination method was as follows: 100 mL of surface V class water (initial COD concentration was 40 mg / L, ammonia nitrogen concentration was 1.7 mg / L) was added to a 250 mL conical flask, and the combined strains were inoculated respectively, and cultured at 30 ° C and 150 r / min for three days. The COD and ammonia nitrogen concentrations before and after culture were determined. COD was determined by potassium dichromate method (HJ828-2017), and ammonia nitrogen was determined by Nessler's reagent spectrophotometry (HJ 535-2009). The COD and ammonia nitrogen removal rate calculation formulas are as follows:

[0050] COD removal rate = (C0-C1) / C0*100%

[0051] Ammonia nitrogen removal rate = (C2-C3) / C2*100%

[0052] In the formula: C0 refers to the COD concentration in the water before the test, C1 refers to the COD concentration in the water at the end of the test, C2 refers to the ammonia nitrogen concentration in the water before the test, and C3 refers to the ammonia nitrogen concentration in the water at the end of the test. In the subsequent determination of COD and ammonia nitrogen removal rates, unless otherwise specified, the methods are the same as here.

[0053] The results of the test are shown in Figure 2 . The results showed that among the COD-degrading bacteria groups, the degradation rates of group F and group Z were more prominent, at 53.47% and 71.10% respectively, which may be related to the strong adsorption capacity of the strains in the two test groups. However, after the combination of group F and group Z strains (group Q), the COD degradation rate decreased, indicating that there may be a certain antagonism between the two groups of microorganisms. Among the nitrifying bacteria groups, the ammonia nitrogen degradation rates of group H and group Y were higher, at 69.00% and 74.43% respectively. However, after the combination of group H and group Y (group B), the ammonia nitrogen degradation rate decreased significantly, indicating that there may be a strong antagonism between the two groups of microorganisms.

[0054] Based on the above results, two mixed bacteria groups, F and Z, with higher COD degradation ability, were selected and combined with two mixed bacteria groups, H and Y, with higher ammonia nitrogen degradation ability, respectively. Specifically, they include: F+H group (F-1, F-2, F-4, F-5, H-1, H-3, H-7), F+Y group (F-1, F-2, F-4, F-5, Y-9, Y-17), Z+H group (Z-2, Z-5, H-1, H-3, H-7) and Z+Y group (Z-2, Z-5, Y-9, Y-17). Similarly, each microorganism was prepared as OD 600 =1 seed solution, and mix equal volumes of each microbial seed solution in groups.

[0055] The COD removal rate and ammonia nitrogen removal rate of each group were tested by the same method. The results are shown in Figure 3 .according to Figure 3 ,Taking into account the degradation rates of COD and ammonia nitrogen, the F+Y group had the best sewage treatment effect, and its strain composition was F-1, F-2, F-4, F-5, Y-9 and Y-17.

[0056] 3. Identification

[0057] DNA of the six strains (F-1, F-2, F-4, F-5, Y-9, and Y-17) in the F+Y group was extracted and used as a template for PCR reaction to perform 16S rRNA gene PCR amplification. The PCR products were sent to Sangon Biotech (Shanghai) Co., Ltd. for sequencing, and the species of each strain were determined by NCBI Blast comparison. The identification results are shown in Table 1. It was identified that F-1 and F-2 were both Pseudomonas pseudoalcaligenes, and Y-9 and Y-17 were both Trichosporon dermatophyte, so the final composition of the inoculum was F-1, F-4, F-5, and Y-9.

[0058] Table 1 Identification results of strain species

[0059] Strain number Strain identification results F-1 Pseudomonas pseudoalcaligenes F-2 Pseudomonas pseudoalcaligenes F-4 Enterobacter cloacae F-5 Kodamaea ohmeri Y-9 Trichosporon cutaneum Y-17 Trichosporon cutaneum

[0060] 4. Relationship between strain dosage

[0061] The optimal ratio experiment of bacterial agents was conducted on F-1, F-4, F-5, and Y-9. The ratio and results are shown in Table 2. In Table 2, each microorganism to be tested was prepared as OD 600 =1 seed solution, and mix the seed solutions of various microorganisms in groups according to the proportion (volume ratio) in Table 2.

[0062] Table 2 Comparison of the effect of microbial dosage on pollutant degradation

[0063] Group F-1 F-4 F-5 Y-9 COD removal rate Ammonia nitrogen removal rate 1 1 1 1 1 43％ 58％ 2 1 2 2 2 57％ 76％ 3 1 3 3 3 63％ 83％ 4 2 1 2 3 72％ 85％ 5 2 2 3 1 75％ 95％ 6 2 3 1 2 74％ 69％ 7 3 1 3 2 64％ 87％ 8 3 2 1 3 58％ 69％ 9 3 3 2 1 65％ 80％

[0064] It can be seen that when the ratio is F-1, F-4, F-5, Y-9 = 2:2:3:1, the obtained microbial composite agent has the best effect, with a COD removal rate of 75% and an ammonia nitrogen removal rate of 95%.

[0065] 5. Strain replacement test

[0066] A strain replacement experiment was conducted on the bacterial agent composed of F-1: F-4: F-5: Y-9 = 2: 2: 3: 1. The corresponding strains of the same species were replaced with purchased Pseudomonas pseudoalcaligenes, Enterobacter cloacae, Omokoda yeast, and Trichosporon dermestii. The experimental results are shown in the table below.

[0067] Table 3 Comparison of the effects of strains on pollutant degradation

[0068] Experimental Group Microbial replacement COD removal rate Ammonia nitrogen removal rate 1 Commercially available Pseudomonas pseudoalcaligenes was used to replace F-1 74％ 95％ 2 Commercially purchased Enterobacter cloacae replaced F-4 67％ 96％ 3 Commercially purchased Omokoda yeast replaces F-5 41％ 67％ 4 Commercially available Trichosporon dermestii was used to replace Y-9 75％ 92％

[0069] The results showed that after replacing with other similar Pseudomonas pseudoalcaligenes, Enterobacter cloacae and Trichosporon dermatophyte, the COD removal rate and ammonia nitrogen removal rate were not much different; but after replacing the Omokoda yeast with other commercially available Omokoda yeast, the COD removal capacity and ammonia nitrogen degradation effect were significantly reduced. Therefore, the Omokoda yeast was preserved and deposited in the China General Microbiological Culture Collection Center (CGMCC) on January 2, 2025. The preservation address is: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is: CGMCC NO.33277.

[0070] The colony morphology of the Omokoda yeast is shown in FIG. Figure 4 As shown, the colony is white, small, and has irregular hyphae. It can grow under pH 3-8 conditions, and the suitable growth conditions are pH=7, 30℃. The results of biolog ecological plate determination showed that the strain has spectral utilization of a variety of basic carbon sources, and the most easily utilized basic carbon sources include D-cellobiose, glucose-1-phosphate, α-D-lactose, and methyl pyruvate.

[0071] Example 2: Screening of water purification plants

[0072] Lophatherum gracile Brongn., Alpinia japonica (Thunb.) Miq., Commelina diffusa Burm.f., Acorus gramineus and Equisetum hyemale L. in the growth period were selected for evaluation and comparison of their pollution removal abilities.

[0073] The potted experiment was conducted with a plant density of 22 plants / m 2, 2 plants / pot, planting depth 12cm, the experiment was carried out for 9 batches, each batch was repeated 3 times, and the results were averaged. Each time, the total nitrogen, total phosphorus, and ammonia nitrogen in the water in the bottom tray of the plant were sampled and measured, and then the prepared experimental water (surface water inferior to Class V, prepared by potassium nitrate and diammonium phosphate) was poured, and the watering amount was 0.4L / pot. Water once every 5 days, each watering is a batch, and a total of 9 waterings are set, that is, a total of 9 batches are set; 5 days after each watering and before the next batch of watering, the total nitrogen, total phosphorus and ammonia nitrogen of the effluent of the batch are measured. Among them, COD was determined by Lovibond COD analyzer according to HJ / T 399-2007 standard rapid digestion spectrophotometry; ammonia nitrogen was determined according to HJ 535-2009 Nessler reagent spectrophotometry; total nitrogen was determined according to HJ 636-2012 alkaline potassium persulfate ultraviolet spectrophotometry; total phosphorus was determined according to GB 11893-89 ammonium molybdate spectrophotometry. All experiments were repeated three times and the results were averaged.

[0074] 1. Total nitrogen. The results are shown in Table 4. During the test, the total nitrogen concentration of each batch of influent was 3.15 mg / L.

[0075] Table 4 Purification effect of five plants on total nitrogen

[0076]

[0077] The results showed that the five terrestrial plants all had a certain purification effect on total nitrogen in water, among which the light bamboo leaves had the best purification effect.

[0078] 2. Total phosphorus. As shown in Table 5, during the test period, the total phosphorus concentration of the influent was 0.8 mg / L.

[0079] Table 5 The purification effect of five plants on total phosphorus

[0080]

[0081] The results showed that the five terrestrial plants all had a certain purification effect on total phosphorus in water, and the purification effect gradually improved with the extension of time. This may be due to the continuous growth of plants. Among them, bamboo leaves and horsetail have the best purification effect.

[0082] 3. Ammonia nitrogen. The results are shown in Table 6. During the test period, the ammonia nitrogen concentration of the influent was 0.75 mg / L.

[0083] Table 6 Purification effect of five plants on ammonia nitrogen

[0084]

[0085] The results showed that the five terrestrial plants all had a certain purification effect on ammonia nitrogen in water, among which the light bamboo leaves had the best purification effect.

[0086] In summary, the light bamboo leaves have the most ideal effect on sewage purification.

[0087] Example 3: Water purification system effect demonstration

[0088] 1. Construction of water purification system: Use LB medium to culture F-1, F-4, F-5, and Y-9 of Example 1 to OD 600 =1 seed liquid, and mix them in a volume ratio of F-1:F-4:F-5:Y-9=2:2:3:1 to obtain microorganisms in the water purification system.

[0089] The biochar and zeolite were crushed into 60 mesh particles, PBS was melted at 120°C, and 28% (w / w) biochar and zeolite particles (equal amounts were mixed), 0.1% (w / w) ZnSO4, 0.4% (w / w) Fe2(SO4)3 and 0.05% (w / w) CuO were added. Stir for 20 minutes and extrude to obtain particle 1 with a diameter of 2 mm. In a 60°C water bath, 2g of sodium alginate, 0.002g of L-aspartic acid, biotin, and cytokinin were added to 100mL of pure water and dissolved to obtain a hydrogel solution. Particle 1 was placed in the hydrogel solution and stirred to fully wrap the colloidal solution on the surface of particle 1 to obtain particle 2. Particle 2 was taken out and placed in a 2% (w / w) CaCl2 curing solution and cured at 4°C for 24h to obtain a carrier.

[0090] The microorganisms and carriers were mixed in a volume ratio of 1:1. The matrix was mixed in a mass ratio of soil: zeolite: ceramsite: volcanic rock = 1:1:1:1, wherein zeolite, ceramsite, and volcanic rock were purchased from Gongyi Miaoyuan Water Treatment Materials Co., Ltd., and the particle size was 8-16 mm. A mixture of microorganisms and carriers was added to the matrix at 0.1% of the matrix mass to obtain a light bamboo leaf planting matrix.

[0091] Selected bamboo leaves in the growth period, about 30 cm tall, and used potted experiments with a plant density of 22 plants / m 2 , planting depth of 12cm, planted in the substrate (2 light bamboo leaves are planted in each pot, and 20kg of substrate is placed in each pot). Each time a sample is taken to measure the total nitrogen, total phosphorus, and ammonia nitrogen in the water in the bottom tray of the plant, and then the prepared experimental water (surface water inferior V class) is poured, and the watering amount is 0.4L / pot. After 5 days, new experimental water is poured, and a total of 8 waterings are poured (corresponding to 8 batches, each batch is repeated 3 times, and the results are averaged). The experimental water for each batch is the same. In the experimental water, the total nitrogen is 3.15mg / L, the ammonia nitrogen is 0.75mg / L, the total phosphorus is 0.8mg / L, and the COD is 40mg / L.

[0092] At the same time, a blank control group was set up with the same other conditions. No microorganism and carrier mixture was added to the substrate, and light bamboo leaves were directly planted.

[0093] Five days after each batch of watering and before the next batch of watering, the bottom tray water was collected and the ammonia nitrogen, total nitrogen, total phosphorus and COD of the effluent were determined. The results are shown in Table 7.

[0094] Table 7 Water purification effect display table of water purification system

[0095]

[0096] The results showed that compared with directly planting light bamboo leaves, the water purification system had significantly improved effects in the treatment of total nitrogen, ammonia nitrogen, total phosphorus and COD, and the improvement effect became more obvious with the increase of time.

[0097] The embodiments described above are only descriptions of the preferred modes of the present invention and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various deformations, modifications, and substitutions made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A strain of Kodamaea ohmeri, characterized in that: The deposit number of the Omokoda yeast is: CGMCC NO.33277.

2. A bacterial preparation prepared using the Omokoda yeast according to claim 1; or; a bacterial preparation comprising the Omokoda yeast according to claim 1.

3. A bacterial preparation, characterized in that: The bacterial preparation comprises Pseudomonas, Enterobacter, Trichosporon and Omokoda yeast, and the preservation number of the Omokoda yeast is: CGMCCNO.33277.

4. The bacterial preparation according to claim 3, characterized in that: The Pseudomonas is Pseudomonas pseudoalcaligenes; and / or; the Enterobacter is Enterobacter cloacae; and / or; the Trichosporon is Trichosporon dermestii.

5. A water purification system, characterized in that: The water purification system comprises the Omokoda yeast according to claim 1, or comprises the bacterial preparation according to any one of claims 2 to 4.

6. The water purification system according to claim 5, characterized in that: The water purification system also includes plants with water purification capabilities.

7. The water purification system according to claim 6, characterized in that: The plants include bamboo leaves.

8. The water purification system according to claim 5, characterized in that: The water purification system also includes a microbial carrier.

9. The method for preparing a water purification system according to any one of claims 5 to 8, characterized in that: The Omokoda yeast, or the bacterial preparation, or the Omokoda yeast / bacterial preparation and a carrier are put into a substrate for planting water purification plants, and then the substrate is used to plant water purification plants, and the water purification plants are planted in sewage to be treated, or the sewage to be treated is used to irrigate the water purification plants.

10. A method for treating sewage using the Omokoda yeast according to claim 1, or the bacterial preparation according to any one of claims 2 to 4, or the water purification system according to any one of claims 5 to 8.

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