Mechanically enhanced PBS fiber membrane and preparation method and application thereof

Abstract

The invention discloses a mechanically-enhanced PBS (Phosphate Buffer Solution) fiber membrane as well as a preparation method and application thereof. The fiber membrane comprises a random fiber membrane and an oriented fiber membrane, and is characterized in that the fiber membrane is composed of PBS fibers added with functional nanoparticles, and the preparation method comprises the following steps: dissolving a polymer PBS and the functional nanoparticles with a specific concentration into a polar organic solvent to obtain a spinning solution, placing the spinning solution in an ultrasonic environment for dispersion treatment, and carrying out ultrasonic treatment to obtain the PBS / PBS composite fiber membrane. And transferring the spinning solution into a spinning needle tube for electrostatic spinning to obtain a random fiber membrane or an oriented fiber membrane. According to the invention, the interaction force between PBS fiber membrane molecules is enhanced by adding the functional nanoparticles with a specific concentration, so that the mechanical enhancement effect is controllable, and compared with a fiber membrane without adding the functional nanoparticles, the provided fiber membrane has more excellent mechanical strength, can better support a damaged part for repairing, and has a good application prospect. The tissue of the damaged part is recovered through various properties, and the method is suitable for clinical application.

Description

Technical Field

[0001] The invention relates to a mechanically enhanced PBS fiber membrane and a preparation method and application thereof, belonging to the technical field of bio-based repair. Background Art

[0002] At present, the field of biomedical materials belongs to the new development field in materials science. It is the basic material for studying artificial organs and medical devices. It is closely related to the life and health of patients and has become a hot spot for research and development by scientists around the world. There are three main types of biomedical materials: one is replacement, which can replace damaged organs and tissues such as dentures; the second is repair, which can repair damaged parts of nerves and skin injuries and restore their functions; the third is auxiliary, which can be used as controlled release materials for drug carriers. When used for tissue engineering scaffolds, these biomedical materials are more polymer materials, among which synthetic materials are the most widely used materials due to their advantages such as low price, easy preparation, and large-scale production.

[0003] Recent studies have found that polybutylene succinate (PBS) is considered to be another choice for biomedical materials due to its inherent biocompatibility, degradability and low price, and fewer studies have also shown this view. However, the study also found that without any modification, the mechanical properties of PBS-based scaffolds are weak and cannot meet the requirements of rotator cuff patches, which require stronger mechanics to support the suture of damaged parts or even long-term implant stability. Therefore, further exploration is necessary. In addition, studies have shown that some functional nanoparticles can enhance the mechanics of polymers. In addition, nanomaterials with specific functions can also play a specific biorepair effect. For example: nanoparticle selenium is not only an essential trace element in the body, but also has high antibacterial and anti-cancer capabilities, which can be used as the first choice for wound dressings; polydopamine has multiple properties such as antioxidant, photothermal conversion, adhesion, biocompatibility and biodegradability, and can be used in neural scaffolds, etc.

[0004] Therefore, it is necessary to propose a mechanically reinforced PBS fiber membrane by adding functional nanoparticles and its preparation method and application to improve the application performance of the PBS fiber membrane. Summary of the invention

[0005] The purpose of the present invention is to solve the above problems and provide a mechanically enhanced PBS fiber membrane and a preparation method and application thereof.

[0006] The technical solution of the present invention is: a mechanically enhanced PBS fiber membrane, the mechanically enhanced PBS fiber membrane includes a random fiber membrane and an oriented fiber membrane, and is characterized in that: the random fiber membrane or the oriented fiber membrane is composed of PBS fibers added with functional nanoparticles. The thickness of the random fiber membrane is 0.150-0.312 mm, and the breaking strength of the random fiber membrane is 4.5-5.72 MPa; the thickness of the oriented fiber membrane is 0.140-0.225 mm, and the breaking strength of the oriented fiber membrane in the oriented direction is 8.97-9.91 MPa, and the breaking strength of the oriented fiber membrane in the non-oriented direction is 2.06-2.23 MPa. The functional nanoparticles are one or more of carbon quantum dots, carbon nanotubes, graphene, Ti3C2Tx, MOF, polydopamine, silicon dioxide, zinc oxide, selenium and boron nitride (that is, the added functional nanoparticles are biomedical grade, and the added functional nanoparticles are single type or mixed type).

[0007] The present invention also provides a method for preparing a mechanically enhanced PBS fiber membrane, comprising the following steps:

[0008] (i) dissolving the polymer PBS and the functional nanoparticles simultaneously in a polar organic solvent to directly obtain a spinning solution, or first dissolving the polymer PBS in a polar organic solvent to obtain a spinning precursor solution, and then mixing the functional nanoparticles in the spinning precursor solution to obtain a spinning solution;

[0009] (ii) placing the spinning solution in an ultrasonic instrument, dispersing the spinning solution through ultrasonic vibration and magnetic field coupling to obtain an electrospinning solution;

[0010] (iii) subjecting the electrospinning solution to electrospinning treatment, loading the electrospinning solution into a syringe, installing the syringe in an electrospinning machine, adjusting the electrospinning parameters for electrospinning, and obtaining a random fiber membrane or an oriented fiber membrane with mechanical enhancement.

[0011] Furthermore, in the above-mentioned method for preparing the mechanically enhanced PBS fiber membrane, the concentration of the functional nanoparticles is 0.05-0.2 wt %.

[0012] Furthermore, in the above-mentioned method for preparing the mechanically enhanced PBS fiber membrane, the polar organic solvent is hexafluoroisopropanol or dichloromethane.

[0013] Furthermore, in the above-mentioned method for preparing the mechanically enhanced PBS fiber membrane, the total concentration of the spinning solution is 6-10wt%.

[0014] Furthermore, in the above-mentioned method for preparing the mechanically enhanced PBS fiber membrane, the injection speed of the spinning solution is 0.7-1.5 mL / h, the voltage is 15-30 KV, and the spinning distance is 15-18 cm; the temperature of the electrospinning is 20-30° C., and the relative humidity is 40-50%.

[0015] Furthermore, in the above-mentioned method for preparing the mechanically enhanced PBS fiber membrane, the drum speed range of the random fiber membrane obtained by electrospinning is 200-800 rpm; the drum speed range of the oriented fiber membrane obtained by electrospinning is 800-5000 rpm.

[0016] The present invention also provides that the mechanically enhanced PBS fiber membrane involved in the above scheme can be used for tissue engineering scaffolds, especially a type of tissue engineering scaffold with relatively high mechanical properties, so as to replace or repair damaged tissues and organs at the biological level.

[0017] Compared with the prior art, after adopting the technical solution of the present invention, on the one hand, polymer PBS has significant advantages in implantable medical devices, including controllable degradation, biocompatibility and material price, etc.; on the other hand, functional nanoparticles can be used as "enhancers" in terms of mechanical strength, so that the intermolecular interaction is strengthened, and can also play a certain repair role by virtue of their own special functions. By setting a specific concentration, the controllability can be further improved, so that the effect is controllable. Therefore, the fiber membrane provided by the present invention has better mechanical strength than the fiber membrane without adding functional nanoparticles. This enhanced mechanical membrane can better support the repair of the damaged part, and restore the tissue and even function of the damaged part through various properties, so as to be clinically applied; moreover, the mechanically enhanced PBS fiber membrane is made by electrostatic spinning, which has significant advantages in preparing tissue engineering scaffolds, and can even disperse functional nanoparticles in the spinning solution of polymer PBS, so as to construct a patch with enhanced mechanics, and then it is expected to further expand the application of PBS in biology. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a structural schematic diagram of the present invention;

[0019] Figure 2 The surface microstructure scanning electron microscope image of comparative example 1 (the scanning electron microscope scale is 5 μm);

[0020] Figure 3 The surface microstructure scanning electron microscope image of comparative example 1 (the scanning electron microscope scale is 1 μm);

[0021] Figure 4 This is a scanning electron microscope image of the surface microstructure of Comparative Example 2 (the scanning electron microscope scale is 5 μm) at high rotation speed;

[0022] Figure 5 This is a scanning electron microscope image of the surface microstructure of Comparative Example 2 (the scanning electron microscope scale is 1 μm) at high rotation speed;

[0023] Figure 6 The surface microstructure scanning electron microscope image of Example 1 (the scanning electron microscope scale is 5 μm);

[0024] Figure 7 The surface microstructure scanning electron microscope image of Example 1 (the scanning electron microscope scale is 1 μm);

[0025] Figure 8 The surface microstructure scanning electron microscope image of Example 2 (the scanning electron microscope scale is 5 μm);

[0026] Fig. 9 The surface microstructure scanning electron microscope image of Example 2 (the scanning electron microscope scale is 1 μm);

[0027] Fig.10 This is a scanning electron microscope image of the surface microstructure under high rotation speed of Example 3 (the scanning electron microscope scale is 5 μm);

[0028] Fig.11 This is a scanning electron microscope image of the surface microstructure under high rotation speed of Example 3 (the scanning electron microscope scale is 1 μm);

[0029] Fig.12 The stress-strain curve of the universal tensile testing machine test of Comparative Example 1;

[0030] Fig.13 The stress-strain curve of the universal tensile testing machine in the orientation direction of Comparative Example 2;

[0031] Fig.14 The stress-strain curve of the universal tensile testing machine in the non-oriented direction of Comparative Example 2;

[0032] Fig.15 This is a stress-strain curve diagram of the universal tensile testing machine test of Example 1;

[0033] Fig.16 This is a stress-strain curve diagram of the universal tensile testing machine test of Example 2;

[0034] Fig.17 The stress-strain curve of the universal tensile testing machine in the orientation direction of Example 3;

[0035] Fig.18 The stress-strain curve of the universal tensile testing machine in the non-oriented direction of Example 3;

[0036] Fig.19Columnar analysis distribution diagram of the maximum fracture stress of Comparative Example 1, Example 1 and Example 2;

[0037] Fig. 20 Columnar analysis distribution diagram of the maximum fracture stress in the orientation direction of Comparative Example 2 and Example 3;

[0038] Fig.21 It is a columnar analysis distribution diagram of the maximum breaking stress in the non-oriented direction of Comparative Example 2 and Example 3. DETAILED DESCRIPTION

[0039] In order to make the purpose, technical scheme and advantages of the present invention more clearly understood, the present invention is further described below in conjunction with specific examples, but it should not be understood that the protection scope of the above subject matter of the present invention is limited to the following examples. Among them, the raw materials used are commercially available or homemade using methods known in the industry.

[0040] Comparative Example 1

[0041] Please refer to the fiber status at different magnifications. Figure 2 and Figure 3 The spinning speed of the random fiber membrane without adding functional nanoparticles in comparative example 1 is low, the fibers are randomly arranged, both sides of the fiber membrane are smooth and free of beads, and the surface fibers are random.

[0042] The specific preparation method of Comparative Example 1 is as follows: 1.39 g of polymer PBS is placed in 10 mL of hexafluoroisopropanol solution, fully stirred in a magnetic stirrer and prepared into a spinning solution with a mass fraction of 8 wt%, the environment in the spinning machine is controlled at a temperature of 33 ° C and a humidity of 53%, and then the spinning solution is transferred into the spinning syringe, the spinning voltage is set to +12 kV, -3 kV, the injection speed is 1 mL / h, the spinning distance is 15 cm, and the drum speed is 500 rpm to obtain a random fiber membrane without adding functional nanoparticles.

[0043] Comparative Example 2

[0044] See also Figure 4 and Figure 5 The spinning speed of the oriented fiber membrane without adding functional nanoparticles in comparative example 2 was high throughout the spinning process, the surface of the oriented fiber membrane was smooth and free of beads, and the fibers showed a high degree of orientation.

[0045] The specific preparation method of Comparative Example 2 is as follows: 1.39 g of polymer PBS is placed in 10 mL of hexafluoroisopropanol solution, fully stirred in a magnetic stirrer and prepared into a spinning solution with a mass fraction of 8 wt%; the environment in the spinning machine is controlled at a temperature of 35°C and a humidity of 50%, and then the spinning solution is transferred into the spinning syringe, the spinning voltage is set to +15 kV, -5 kV, the injection speed is 1 mL / h, the spinning distance is 15 cm, and the drum speed is 2000 rpm to obtain an oriented fiber membrane without adding functional nanoparticles.

[0046] Example 1

[0047] Please refer to the fiber status at different magnifications. Figure 6 and Figure 7 The spinning speed of the random fiber membrane with added carbon quantum dots in Example 1 is low, the fibers are randomly arranged, both sides of the fiber membrane are smooth and free of beads, the surface fibers are random, and the diameter of the fibers decreases with the addition of functional nanoparticles.

[0048] The specific preparation method of Example 1 is as follows: 1.39 g of polymer PBS is placed in 10 mL of hexafluoroisopropanol solution, which is fully stirred in a magnetic stirrer to prepare a spinning precursor solution with a mass fraction of 8 wt%, and carbon quantum dots are placed in the spinning precursor solution for ultrasonic dispersion and fully stirred to a concentration of 0.08 wt%; the prepared carbon quantum dot spinning solution is transferred into the spinning syringe, the spinning voltage is set to +12 kV, -3 kV, the injection speed is 1.0 mL / h, the spinning distance is 15 cm, and the drum speed is set to 500 rpm to obtain a random fiber membrane with added carbon quantum dots.

[0049] Example 2

[0050] Please refer to the fiber status at different magnifications. Figure 8 and Fig. 9 The spinning speed of the random fiber membrane with added carbon quantum dots in Example 2 is low, the fibers are randomly arranged, both sides of the fiber membrane are smooth and free of beads, the surface fibers are random, and the diameter of the fibers decreases with the addition of functional nanoparticles.

[0051] The specific preparation method of Example 2 is as follows: 1.39 g of polymer PBS is placed in 10 mL of hexafluoroisopropanol solution, which is fully stirred in a magnetic stirrer to prepare a spinning precursor solution with a mass fraction of 8 wt%, and carbon quantum dots are placed in the spinning precursor solution for ultrasonic dispersion and fully stirred to a concentration of 0.1 wt%; the prepared carbon quantum dot spinning solution is transferred into the spinning syringe, the spinning voltage is set to +12 kV, -3 kV, the injection speed is 1.0 mL / h, the spinning distance is 15 cm, and the drum speed is set to 500 rpm to obtain a random fiber membrane with added carbon quantum dots.

[0052] Example 3

[0053] See also Fig.10 and Fig.11 The spinning speed of the oriented fiber membrane without adding functional nanoparticles in comparative example 2 was high throughout the spinning process, the surface of the fiber membrane was smooth and free of beads, and the fibers showed a high degree of orientation.

[0054] The specific preparation method of Example 3 is as follows: 1.39 g of polymer PBS is placed in 10 mL of hexafluoroisopropanol solution, which is fully stirred in a magnetic stirrer to prepare a spinning precursor solution with a mass fraction of 8 wt%, and carbon quantum dots are placed in the spinning precursor solution for ultrasonic dispersion and fully stirred to a concentration of 0.08 wt%; the prepared carbon quantum dot spinning solution is transferred into the spinning syringe, the spinning voltage is set to +15 kV, -5 kV, the injection speed is 1.0 mL / h, the spinning distance is 15 cm, and the drum speed is set to 2000 rpm to obtain an oriented fiber membrane with added carbon quantum dots.

[0055] Example 4

[0056] The specific preparation method is as follows: 1.39g of polymer PBS is placed in 10mL of dichloromethane solution, and the solution is fully stirred in a magnetic stirrer to prepare a spinning precursor solution with a mass fraction of 8wt%, and carbon nanotubes are placed in the spinning precursor solution for ultrasonic dispersion and fully stirred to a concentration of 0.05wt%; the prepared carbon nanotube spinning solution is transferred into a spinning syringe, and the spinning voltage is set to +12kV, -3kV, the injection speed is 1.0mL / h, the spinning distance is 15cm, and the drum speed is set to 500rpm to obtain a random fiber membrane with added carbon nanotubes.

[0057] Example 5

[0058] The specific preparation method is as follows: 1.39g of polymer PBS is placed in 10mL of dichloromethane solution, and the solution is fully stirred in a magnetic stirrer to prepare a spinning precursor solution with a mass fraction of 8wt%, and carbon nanotubes are placed in the spinning precursor solution for ultrasonic dispersion and fully stirred to a concentration of 0.08wt%; the prepared carbon nanotube spinning solution is transferred into a spinning syringe, and the spinning voltage is set to +15kV, -5kV, the injection speed is 1.0mL / h, the spinning distance is 15cm, and the drum speed is set to 2000rpm to obtain an oriented fiber membrane with added carbon nanotubes.

[0059] 1. Performance test and structural characterization of fiber membranes with added carbon quantum dots:

[0060] The following experiments will be used to verify other properties of the fiber membranes provided in Examples 1 to 3 of the present invention.

[0061] Please see attached Figure 1 , this embodiment is a mechanically reinforced PBS fiber membrane with added carbon quantum dots.

[0062] Please see attached Figures 2 to 11 , The membranes prepared under different parameters were observed by scanning electron microscopy to determine the complete structure of the fibers in the membranes.

[0063] In accordance with GB / T3923.1, the random fiber membranes without added functional nanoparticles and the random fiber membranes with added carbon quantum dots prepared in Comparative Examples 1 to 2 and Examples 1 to 3 were prepared into dumbbell shapes with a length and width of 20×5 mm. The thickness of the membrane was accurately measured, and the mechanical strength and elongation at break of the membrane were calculated based on the relevant data.

[0064] Table 1 Mechanical properties test results of comparative examples 1 to 2 and embodiments 1 to 3

[0065]

[0066] from Fig.12 , Fig.15 and Fig.16 From Table 1, it can be seen that the maximum breaking strength of Comparative Example 1 is 3.44 MPa, which is much lower than the breaking strength of the fiber membranes of Examples 1 and 2, indicating that the addition of carbon quantum dots as functional nanoparticles is beneficial to the improvement of the mechanical strength of the fiber membrane.

[0067] from Fig.13 , Fig.14 , Fig.17 and Fig.18 From Table 1, it can be seen that the maximum breaking strength of the fiber membrane obtained in Comparative Example 2 in the oriented direction is 6.29 MPa, and the maximum breaking strength in the non-oriented direction is 1.13 MPa, while the maximum breaking strength of the fiber membrane obtained in Example 3 in the oriented direction is 8.90 MPa, and the maximum breaking strength in the non-oriented direction is 2.16 MPa, indicating that a specific concentration of functional nanoparticles can effectively improve the tensile strength of PBS fiber membranes, which can be better applied to tissue engineering.

[0068] Fig.19 , Fig. 20 and Fig.21 The results in Table 1 show that the overall tensile strength of Examples 1 and 2 is much higher than that of Comparative Example 1, and the mechanical strength of Example 3 in different directions is much higher than that of Comparative Example 2, which is significantly enhanced. Therefore, by introducing functional nanoparticles (i.e., carbon quantum dots) at a specific concentration (i.e., 0.08wt%, 0.1wt%), the breaking strength of the PBS fiber membrane can be improved, thereby fundamentally solving the tissue repair failure caused by poor mechanical properties.

[0069] 2. Performance test and structural characterization of fiber membranes with added carbon nanotubes:

[0070] The following experiments will be used to verify other properties of the fiber membranes provided in Examples 4 to 5 of the present invention.

[0071] According to GB / T3923.1, the random fiber membranes without added functional nanoparticles and the random fiber membranes with added carbon quantum dots prepared in Comparative Examples 1 to 2 and Examples 4 to 5 were prepared into dumbbell shapes with a length and width of 20×5 mm. The thickness of the membrane was accurately measured, and the mechanical strength and elongation at break of the membrane were calculated based on the relevant data.

[0072] Table 2 Mechanical properties test results of Comparative Examples 1-2 and Examples 4-5

[0073]

[0074] It can be seen from Table 2 that the maximum breaking strength of Comparative Example 1 is 3.44 MPa, which is much lower than the breaking strength of the fiber membrane of Example 4. Among them, carbon nanotubes have extremely high tensile strength and elastic modulus, and the strength per unit mass is more than 100 times that of steel. At the same time, they have good flexibility and fatigue resistance, indicating that the addition of carbon nanotubes as functional nanoparticles is beneficial to the improvement of the tensile strength of the fiber membrane, and can further maintain the stability of the fiber membrane structure; the maximum breaking strength of the fiber membrane obtained in Comparative Example 2 in the oriented direction is 6.29 MPa, and the maximum breaking strength in the non-oriented direction is 1.13 MPa, while the maximum breaking strength of the fiber membrane obtained in Example 5 in the oriented direction is 9.10 MPa, and the maximum breaking strength in the non-oriented direction is 2.22 MPa, indicating that carbon nanotubes with a concentration of 0.05wt% and 0.08wt% can effectively improve the mechanical strength of the PBS fiber membrane, thereby proving that the introduction of functional nanoparticles at a specific concentration can significantly improve the breaking strength of the PBS fiber membrane, which can be better applied to tissue engineering to achieve the effect of replacing or repairing damaged tissues and organs at the biological level.

[0075] In summary, in the technical solution of the present invention, the PBS fiber membrane of this case is prepared by adding functional nanoparticles and using a specific concentration to enhance mechanics, which is the technical key of this case, improving the controllability of preparation, and improving the tensile strength and breaking strength of the PBS fiber membrane. For the hexafluoroisopropanol solution and the dichloromethane solution, ordinary technicians in this field can make conventional proportions according to the existing technology, and this case has no special requirements for the selection of its components and the preparation and use of reagents.

[0076] Through the above description, it can be found that compared with the prior art, after adopting the technical solution of the present invention, which is composed of polymer PBS and multifunctional nanoparticles, on the one hand, polymer PBS has significant advantages in implantable medical devices, including controllable degradation, biocompatibility and material price, etc.; on the other hand, functional nanoparticles can be used as "enhancers" in terms of mechanical strength to strengthen the interaction between molecules, and can also play a certain repair role by virtue of their own special functions. Therefore, the fiber membrane provided by the present invention has better mechanical strength than the fiber membrane without adding functional nanoparticles. This membrane with enhanced mechanics can better support the repair of the damaged part, and restore the tissue and even function of the damaged part through various properties, and is expected to be clinically applied.

[0077] The technical scheme, working process and implementation effect of the present invention are described in detail above. It should be noted that what is described is only a typical example of the present invention. In addition, the present invention may also have many other specific implementation methods. Any technical scheme formed by equivalent replacement or equivalent transformation falls within the scope of protection required by the present invention.

Claims

1. A mechanically enhanced PBS fiber membrane, the mechanically enhanced PBS fiber membrane comprising a random fiber membrane and an oriented fiber membrane, characterized in that: The random fiber membrane or the oriented fiber membrane is composed of PBS fibers added with functional nanoparticles; The thickness of the random fiber membrane is 0.150-0.312 mm, and the breaking strength of the random fiber membrane is 4.5-5.72 MPa; The thickness of the oriented fiber membrane is 0.140-0.225 mm, and the breaking strength of the oriented fiber membrane in the oriented direction is 8.97-9.91 MPa, and the breaking strength of the oriented fiber membrane in the non-oriented direction is 2.06-2.23 MPa; The functional nanoparticles are one or more of carbon quantum dots, carbon nanotubes, graphene, Ti3C2Tx, MOF, polydopamine, silicon dioxide, zinc oxide, selenium and boron nitride.

2. A method for preparing a mechanically enhanced PBS fiber membrane, characterized in that: The following steps are involved: Step S1: dissolving the polymer PBS and the functional nanoparticles in a polar organic solvent at the same time to directly obtain a spinning solution, or first dissolving the polymer PBS in a polar organic solvent to obtain a spinning precursor solution, and then placing the functional nanoparticles in the spinning precursor solution and mixing them to obtain a spinning solution; Step S2: placing the spinning solution obtained in step S1 in an ultrasonic instrument, dispersing the spinning solution through ultrasonic vibration and magnetic field coupling to obtain an electrospinning solution; Step S3: electrospinning the electrospinning solution obtained in step S2, loading the electrospinning solution into a syringe, installing the syringe in an electrospinning machine, adjusting the electrospinning parameters for electrospinning, and obtaining a random fiber membrane or an oriented fiber membrane with mechanical enhancement.

3. The method for preparing the mechanically enhanced PBS fiber membrane according to claim 2, wherein: The concentration of the functional nanoparticles in step 1 is 0.05-0.2 wt %.

4. The method for preparing the mechanically enhanced PBS fiber membrane according to claim 2, wherein: The polar organic solvent in step 1 is hexafluoroisopropanol or dichloromethane.

5. The method for preparing the mechanically enhanced PBS fiber membrane according to claim 2, wherein: The total concentration of the spinning solution in step S1 or step S2 is 6-10 wt %.

6. The method for preparing the mechanically enhanced PBS fiber membrane according to claim 6, wherein: The injection speed of the spinning solution in step S1 or step S2 is 0.7-1.5 mL / h, the voltage is 15-30 KV, and the spinning distance is 15-18 cm; the temperature of the electrostatic spinning is 20-30° C., and the relative humidity is 40-50%.

7. The method for preparing the mechanically enhanced PBS fiber membrane according to claim 2, wherein: The drum speed range of the random fiber membrane obtained by electrospinning in step S3 is 200-800 rpm.

8. The method for preparing the mechanically enhanced PBS fiber membrane according to claim 2, characterized in that: The drum speed range of the oriented fiber membrane obtained by electrostatic spinning in step S3 is 800 to 5000 rpm.

9. Use of the mechanically enhanced PBS fiber membrane according to claim 1 in a tissue engineering scaffold to replace or repair damaged tissues and organs at the biological level.

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