A device for measuring the length of wheat coleoptile in a simulated soil environment for salt tolerance test

Abstract

The present application relates to the technical field of wheat coleoptile length measurement in simulated soil test, and discloses a wheat coleoptile length measurement device in simulated soil environment for salt tolerance test, which comprises a device main body, the device main body comprises a simulated cultivation pool at the middle position and liquid supplement tanks on both sides of the simulated cultivation pool, the inner walls of the liquid supplement tanks and the simulated cultivation pool are uniformly provided with partition plates, and the bottoms of the liquid supplement tanks on both sides of the simulated cultivation pool are fixedly connected with cavities. The present application simulates the soil environment by setting multiple layers of simulated filter paper soaked with wheat culture solution, and realizes real-time supplement of the wheat culture solution on the multiple layers of simulated filter paper through the liquid supplement opening. The growth state of the wheat seeds in the salt tolerance state is recorded in an undamaged state by clamping and fixing filter paper clamping frames on the periphery of the multiple layers of simulated filter paper. The device is more suitable for taking out and observing the growth state of the wheat by additionally arranging rubber plate plugs and push plates at the liquid supplement opening.

Description

TECHNICAL FIELD

[0001] The application relates to a wheat coleoptile length measuring device in a simulated soil environment for salt tolerance test. BACKGROUND

[0002] Wheat has weak salt tolerance, and becomes a limiting factor for wheat production in many saline soil areas in the world. With the contradiction between the demand and supply of wheat becoming more and more prominent, it has become an urgent matter to cultivate wheat varieties with high yield and salt resistance and other excellent traits. However, wheat salt tolerance is a relatively complex quantitative trait controlled by multiple genes, leading to a long research period and large workload in wheat molecular genetic research. Meanwhile, since salt tolerance belongs to a quantitative trait composed of multiple traits, it further increases the difficulty of wheat salt tolerance gene mining. Although many laboratories screen out wheat varieties with strong salt tolerance characteristics by measuring the aboveground fresh weight, aboveground dry weight, root fresh weight, root dry weight, lateral root number and other methods, the salt tolerance traits are difficult to stably inherit, and there is still a big gap in cultivating salt-tolerant wheat varieties with agricultural production value and large-area promotion to promote the development and utilization of saline-alkali land.

[0003] During the whole process of wheat seed germination in soil, the coleoptile has stronger emergence ability than the embryo, can protect the salt-damaged embryo from damage when emerging, and the longer coleoptile is more conducive to the emergence and growth of wheat in saline soil areas. Meanwhile, the heritability of wheat coleoptile length is strong, so the measurement of wheat coleoptile length of different varieties in a simulated saline soil environment can realize rapid selection of wheat salt tolerance indicators, and also utilize the stable inheritance of salt tolerance traits to offspring. In order to facilitate the cultivation of wheat and the observation of wheat coleoptile growth, the application provides a wheat coleoptile length measuring device in a simulated soil environment for salt tolerance test. SUMMARY

[0004] In view of the deficiencies in the prior art, the application provides a wheat coleoptile length measuring device in a simulated soil environment for salt tolerance test, which solves the problem of inconvenient cultivation and observation of wheat coleoptile growth.

[0005] The objective of this invention is achieved as follows: A device for measuring the length of wheat coleoptiles in a simulated soil environment for salt tolerance testing includes a main body. The main body includes a simulated cultivation tank in the middle and replenishment tanks on both sides. The inner walls of the replenishment tanks and the simulated cultivation tank are uniformly provided with partition plates. The bottoms of the replenishment tanks on both sides of the simulated cultivation tank are fixedly connected to cavities. These cavities allow the two sets of replenishment tanks on both sides of the simulated cultivation tank to be interconnected, thus ensuring consistent internal liquid levels. The inner wall of the simulated cultivation tank is fixedly installed with slots located on both sides of the partition plates. Replenishment ports are opened within the grooves of the slots, and flow-limiting devices are installed inside the replenishment ports. The flow-limiting component prevents a large amount of culture medium from flowing into the simulated culture tank, thus avoiding flooding of the wheat seeds. Inside the simulated culture tank, a filter paper frame is movably engaged via a slot. A flow guide tube is fixedly installed inside the filter paper frame. A slit communicating with the flow guide tube is opened on the inner side of the filter paper frame, and simulated filter paper is fixedly engaged through the slit. Both sides of the filter paper frame are fixedly connected to retaining rings, and lifting rods are movably sleeved inside each retaining ring. A lifting hook is fixedly installed at the bottom end of each lifting rod. The two lifting rods are connected by an elastic component, which controls the engagement state between the lifting hook and the retaining ring.

[0006] Furthermore, an opening is provided at the connection between the replenishment tank and the cavity, and the replenishment tank is connected to the simulated culture tank through the replenishment port, the middle of which is cylindrically grooved.

[0007] Furthermore, the flow limiting component includes a flow limiting shaft, the two ends of which are mounted on the central axis of the inner wall of the liquid replenishment port via coil springs. Rubber plate plugs are fixedly connected to both sides of the outer surface of the flow limiting shaft, and a lever is fixedly connected to the side of the flow limiting shaft near the simulated culture tank.

[0008] Furthermore, the filter paper card frame can slide up and down along the card slot, and the position of the liquid replenishment port is aligned with the position of the guide tube.

[0009] Furthermore, the elastic component includes a telescopic sleeve, which is movably sleeved between the tops of the two lifting rods. A spring is movably installed inside the telescopic sleeve, and the two ends of the spring are respectively fixedly connected to the two lifting rods. A pulling rope located below the telescopic sleeve is connected between the two lifting rods.

[0010] Furthermore, the lifting rod is pushed by the spring force, the pulling rope is in a straight state, and the hook at the bottom of the lifting rod hooks the retaining ring.

[0011] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0012] 1. The application simulates the soil environment by setting multiple layers of simulated filter paper soaked in wheat culture solution, and achieves real-time replenishment of the wheat culture solution on the multiple layers of simulated filter paper through the liquid supplementing port; the peripheral carding and fixing of the filter paper card frame on the multiple layers of simulated filter paper achieves recording of the salt-tolerant growth state of the wheat seeds without damage; the rubber plate plug and the poking piece added at the liquid supplementing port make the device more suitable for taking out and observing the growth state of the wheat.

[0013] 2. The application simulates the external environment of wheat germination under soil environment by setting multiple layers of simulated filter paper soaked in wheat culture solution, and achieves real-time replenishment of the wheat culture solution on the multiple layers of simulated filter paper through the liquid supplementing port and the liquid supplementing tank outside the simulated cultivation tank, thereby improving the efficiency of the simulated soil environment salt-tolerance test and reducing the complicated test steps of frequent replenishment of the culture solution by the operator.

[0014] 3. The peripheral carding and fixing of the filter paper card frame on the multiple layers of simulated filter paper, and the simultaneous taking out of multiple groups of filter paper card frames to the outside of the simulated cultivation tank through the lifting rod, and the observation and recording of the growth state of the wheat embryo and coleoptile through the transparent filter paper card frame border, avoid damage to the wheat seeds when taking them out to observe the growth data under soil environment, and achieve recording of the salt-tolerant growth state of the wheat seeds without damage.

[0015] 4. The rubber plate plug and the poking piece added at the liquid supplementing port, and the opening of the rubber plate plug through the pressing of the poking piece by the filter paper card frame border, and the automatic closing of the rubber plate plug when the filter paper card frame is taken out for observation, avoid the flooding effect of a large amount of culture solution in the liquid supplementing tank flowing into the simulated cultivation tank and causing damage to the wheat seeds in the simulated cultivation tank, and make the device more suitable for taking out and observing the growth state of the wheat. DETAILED DESCRIPTION

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or the prior art description. Obviously, the drawings in the following description are only embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor on the basis of the provided drawings.

[0017] Figure 1 It is a schematic diagram of the overall structure of the present application.

[0018] Figure 2 It is a schematic diagram of the vertical section of the overall structure of the present application.

[0019] Figure 3 It is a schematic diagram of the horizontal section of the overall structure of the present application.

[0020] Figure 4 It is a schematic diagram of the local enlargement of the structure at the liquid supplementing port of the present application.

[0021] In the diagram: 1. Main body of the device; 2. Liquid replenishment tank; 201. Cavity; 202. Inlet; 3. Simulated culture tank; 301. Slot; 302. Liquid replenishment port; 4. Divider plate; 5. Filter paper frame; 501. Guide tube; 502. Simulated filter paper; 503. Snap ring; 6. Lifting rod; 601. Telescopic sleeve; 602. Pulling rope; 7. Flow limiting shaft; 701. Rubber plate stopper; 702. Paddle. Detailed Implementation

[0022] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] like Figures 1-4 The device shown is a wheat coleoptile length measuring device under simulated soil conditions for salt tolerance testing. It includes a main body 1, which comprises a simulated cultivation tank 3 in the center and replenishment tanks 2 on either side. The inner walls of the replenishment tanks 2 and the simulated cultivation tank 3 are uniformly equipped with partition plates 4. The bottoms of the replenishment tanks 2 on both sides of the simulated cultivation tank 3 are fixedly connected to cavities 201, allowing the two sets of replenishment tanks 2 on both sides of the simulated cultivation tank 3 to be interconnected, thus ensuring consistent internal liquid levels. The inner wall of the simulated cultivation tank 3 is fixedly installed with slots 301 located on both sides of the partition plates 4. Replenishment ports 302 are opened in the grooves of the slots 301, connecting the replenishment tanks 2 and the simulated cultivation tank 3. This allows the culture medium in the replenishment tanks 2 to enter the simulated cultivation tank 3 through the replenishment ports 302. A flow-limiting component is installed inside the replenishment ports 302 to prevent a large amount of culture medium from flowing into the simulated cultivation tank 3, thereby avoiding damage to the wheat seeds. In the simulated incubation tank 3, a filter paper frame 5 is movably connected to the interior of the tank via a slot 301. A guide pipe 501 is fixedly installed inside the filter paper frame 5. A slit communicating with the guide pipe 501 is opened on the inner side of the filter paper frame 5, and simulated filter paper 502 is fixedly connected to it via the slit. This ensures that when the culture medium in the replenishment tank 2 enters the simulated incubation tank 3 through the replenishment port 302, it must first enter the guide pipe 501 inside the filter paper frame 5 through the replenishment port 302, and then pass through the filter paper frame 502. The slits on the inside allow the culture medium to seep into the simulated filter paper 502, simulating the nutritional environment of wheat germ in soil. Both sides of the filter paper frame 5 are fixedly connected with retaining rings 503. Inside each retaining ring 503, a lifting rod 6 is movably sleeved. The lifting rod 6 is shaped like a "7". A hook is fixedly installed at the bottom of the lifting rod 6. The two lifting rods 6 are connected by an elastic component. The elastic component can control the engagement state between the hook on the lifting rod 6 and the retaining ring 503.

[0024] Further, the connecting part of the liquid supplement tank 2 and the cavity 201 is provided with an opening 202, and the liquid supplement tank 2 and the simulation cultivation tank 3 are communicated through a liquid supplement port 302, and the middle part of the liquid supplement port 302 is cylindrically slotted.

[0025] Further, the flow limiting assembly comprises a flow limiting rotating shaft 7, both ends of the flow limiting rotating shaft 7 are arranged on the central axis of the inner wall of the liquid supplement port 302 through disc springs, and both sides of the outer surface of the flow limiting rotating shaft 7 are fixedly connected with rubber plate plugs 701, and the side of the flow limiting rotating shaft 7 close to the simulation cultivation tank 3 is fixedly connected with a push piece 702.

[0026] When the push piece 702 is pressed downward, the flow limiting rotating shaft 7 is pushed to rotate and open the rubber plate plug 701, and when the push piece 702 is not forced, the disc springs at both ends of the flow limiting rotating shaft 7 drive the flow limiting rotating shaft 7 to reverse and close the rubber plate plug 701 to close the liquid supplement port 302, so that the liquid supplement port 302 communicated with the liquid supplement tank 2 is closed in the normal state of the clamping groove 301, and only when the filter paper clamping frame 5 is pushed inwards, the liquid supplement port 302 is opened and conducted under the pressure of the filter paper clamping frame 5, so as to avoid the large amount of culture solution in the liquid supplement tank 2 from flowing into the simulation cultivation tank 3 to cause the flooding effect on the wheat seeds in the simulation cultivation tank 3.

[0027] Further, the filter paper clamping frame 5 can slide up and down along the clamping groove 301, and the position of the liquid supplement port 302 is aligned with the position of the flow guide pipe 501.

[0028] Further, the elastic assembly comprises a telescopic sleeve 601, the telescopic sleeve 601 is movably sleeved between the top parts of the two side pull rods 6, a spring is movably installed in the telescopic sleeve 601, and both ends of the spring are fixedly connected with the two side pull rods 6, and a pulling rope 602 is connected between the two side pull rods 6 and located below the telescopic sleeve 601.

[0029] Further, the pull rod 6 is pushed by the spring force, the pulling rope 602 is in a straight state, and the lifting hook at the bottom end of the pull rod 6 hooks the clasp 503.

[0030] When the pulling rope 602 is in normal straight state, the lifting rod 6 is pushed out by the spring and everted, the hook at the bottom end of the lifting rod 6 hooks the clasp 503, at this time, the telescopic sleeve 601 is pulled upward to pull the whole set of filter paper card frame 5 upward to the outside of the simulated cultivation tank 3, so that the operator can observe the growth state of the wheat germ from the side, and observe the number of layers of the simulated filter paper 502 in the set of filter paper card frame 5 which is punctured by the wheat germ sheath growth, after the observation is completed, the telescopic sleeve 601 is lowered to put the set of filter paper card frame 5 back into the simulated cultivation tank 3, the pulling rope 602 is lifted to pull the lifting rod 6 to the middle, at this time, the hook at the bottom end of the lifting rod 6 is separated from the clasp 503, the pulling rope 602 is easily taken out by being pulled upward, the two ends of the telescopic sleeve 601 are provided with limiting devices to prevent the lifting rod 6 from being separated from the telescopic sleeve 601 by the spring force after being taken out, at this time, the lifting rod 6 can be inserted into the clasp 503 on both sides of other set of filter paper card frame 5 to carry out observation operation, under the premise of not damaging the wheat germ, the growth state of the wheat in the simulated soil environment is measured conveniently, the growth ability of the germ sheath is recorded quickly by recording the damaged layers of the simulated filter paper 502, so that the high salt tolerance index of the wheat is obtained, and the high heritability of the flood tolerance is obtained.

[0031] The working principle of the use method of the present application is as follows:

[0032] In use, respectively inject gradient salinity wheat culture solution into each group of liquid supplement tank 2 separated by partition plate 4, the liquid level in each group of two side liquid supplement tank 2 is consistent under the connection of bottom cavity 201, at this time, the liquid supplement port 302 connected with simulation cultivation tank 3 is closed by rubber plate plug 701 under the action of coil spring because the toggle 702 is not pressed, then push a piece of filter paper card frame 5 with simulation filter paper 502 into each group of simulation cultivation tank 3 at a time, in the process of pushing, the frame of filter paper card frame 5 presses the toggle 702 in the recessed groove of clamping slot 301 from top to bottom in turn, which makes the liquid supplement port 302 on the way open to the water inlet pipe 501 in turn and then close, until the filter paper card frame 5 falls to the lowest end, continuously press the toggle 702 in the lowest liquid supplement port 302 to make it connected, first enter the water inlet pipe 501 in the filter paper card frame 5 through the liquid supplement port 302, then seep into the simulation filter paper 502 through the gap inside the filter paper card frame 5 to make the simulation filter paper 502 soak in the culture solution, at this time, the culture solution does not flow out of the gap under the action of water surface tension, but continues to supplement when the culture solution on the simulation filter paper 502 evaporates or is consumed, after the last layer of simulation filter paper 502 is soaked in the wheat culture solution, place the wheat to be tested and detected uniformly on the last layer of simulation filter paper 502, then place multiple groups of filter paper card frame 5 on the upper side in each group of simulation cultivation tank 3 to simulate the dark pressure environment in the soil environment, open the liquid supplement port 302 of each group of filter paper card frame 5 to supplement the culture solution to the simulation filter paper 502 to simulate the nutrient environment in the soil, during the cultivation process, use the lifting rod 6 to take out each group of filter paper card frame 5 regularly for observation, in the process of taking out, the vertical section of the lifting rod 6 is inserted into the clasp ring 503 at both ends of the filter paper card frame 5 until the lowest clasp ring 503, the lifting rod 6 is turned out under the action of the spring when the pulling rope 602 is in normal straight state, the lifting hook at the bottom end of the lifting rod 6 hooks the clasp ring 503 at this position, at this time, pulling the telescopic sleeve 601 upwards can pull out the whole filter paper card frame 5 upwards to the outside of the simulation cultivation tank 3, which is convenient for the operator to observe the growth state of the wheat embryo from the side and observe the number of layers of the simulation filter paper 502 in the filter paper card frame 5 punctured by the growth of the wheat embryo sheath, the toggle 702 is automatically closed when it is not pressed in the process of taking out the filter paper card frame 5, which avoids the problem that the liquid supplement port 302 connects the liquid supplement tank 2 and the simulation cultivation tank 3 during the observation process, which causes the simulation cultivation tank 3 to be filled with culture solution, after observation, put the filter paper card frame 5 back into the simulation cultivation tank 3 by lowering the telescopic sleeve 601, pull the lifting rod 6 to make it close to the middle, at this time, the lifting hook at the bottom end of the lifting rod 6 is separated from the clasp ring 503, pulling the lifting rope 602 upwards can easily take it out, similarly, observe and record the wheat growth data in each group of simulation cultivation tank 3 in turn, which avoids the problem that taking out and observing the record in the soil environment causes irreversible damage to the wheat embryo.

[0033] The above description of the embodiments is only used to help understand the method of the present application and its core idea. It should be noted that, for those skilled in the art, some improvements and modifications can be made to the present application without departing from the principles of the present application, and these improvements and modifications also fall within the protection scope of the claims of the present application.

Claims

1. A device for measuring the length of wheat coleoptiles in simulated soil conditions for salt tolerance testing, comprising a main body (1), characterized in that: The main body (1) of the device includes a simulated culture tank (3) in the middle and replenishment tanks (2) on both sides. The inner walls of the replenishment tanks (2) and the simulated culture tank (3) are uniformly provided with partition plates (4). The bottom of the replenishment tanks (2) on both sides of the simulated culture tank (3) is fixedly connected to a cavity (201). The cavity (201) enables the two sets of replenishment tanks (2) on both sides of the simulated culture tank (3) to be interconnected so that the internal liquid level is consistent. The inner wall of the simulated culture tank (3) is fixedly installed with slots (301) located on both sides of the partition plates (4). The grooves of the slots (301) are provided with replenishment ports (302). The replenishment ports (302) are provided with flow limiting components inside. The flow limiting components can prevent a large amount of culture medium from flowing into the replenishment tanks (2). Inside the simulated cultivation pool (3), a filter paper frame (5) is movably connected to the inside of the simulated cultivation pool (3) through a slot (301). A guide pipe (501) is fixedly installed inside the filter paper frame (5). A slit communicating with the guide pipe (501) is opened on the inside of the filter paper frame (5), and simulated filter paper (502) is fixedly connected through the slit. Both sides of the filter paper frame (5) are fixedly connected with a retaining ring (503). A lifting rod (6) is movably sleeved inside the retaining ring (503) on both sides. A lifting hook is fixedly installed at the bottom of the lifting rod (6). The two lifting rods (6) are connected by an elastic component. The elastic component can control the engagement state between the lifting hook on the lifting rod (6) and the retaining ring (503).

2. The device for measuring the length of wheat coleoptiles in simulated soil environment for salt tolerance testing according to claim 1, characterized in that: The connection between the replenishment tank (2) and the cavity (201) is provided with an opening (202). The replenishment tank (2) and the simulated culture pool (3) are connected through a replenishment port (302). The middle part of the replenishment port (302) is cylindrically slotted.

3. The device for measuring the length of wheat coleoptiles in simulated soil environment for salt tolerance testing according to claim 1, characterized in that: The flow limiting component includes a flow limiting shaft (7), the two ends of which are mounted on the central axis of the inner wall of the liquid replenishment port (302) via coil springs. Rubber plate plugs (701) are fixedly connected to both sides of the outer surface of the flow limiting shaft (7), and a lever (702) is fixedly connected to the side of the flow limiting shaft (7) near the simulated culture tank (3).

4. The device for measuring the length of wheat coleoptiles in simulated soil environment for salt tolerance testing according to claim 1, characterized in that: The filter paper frame (5) can slide up and down along the slot (301), and the position of the liquid replenishment port (302) is aligned with the position of the guide tube (501).

5. The device for measuring the length of wheat coleoptiles in simulated soil environment for salt tolerance testing according to claim 1, characterized in that: The elastic component includes a telescopic sleeve (601), which is movably sleeved between the tops of the two lifting rods (6). A spring is movably installed inside the telescopic sleeve (601), and the two ends of the spring are respectively fixedly connected to the two lifting rods (6). A pulling rope (602) located below the telescopic sleeve (601) is connected between the two lifting rods (6).

6. The device for measuring the length of wheat coleoptiles in simulated soil environment for salt tolerance testing according to claim 5, characterized in that: The lifting rod (6) is pushed by the spring force, the pulling rope (602) is in a straight state, and the hook at the bottom of the lifting rod (6) hooks the retaining ring (503).

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