On-site water sample collection device and method based on solid-phase extraction technology

Abstract

The invention relates to an on-site water sample collection device and method based on a solid-phase extraction technology, and the device comprises a collection box and a sampling assembly, and the surface of the collection box is provided with a water inlet and a water outlet; the water inlet is connected with a water inlet hose, and the water outlet is connected with a water outlet hose; a filter is arranged between the water inlet hose and the water inlet; the collection assembly comprises a solid-phase extraction module, a first sealing joint, a second sealing joint and a constant-temperature box, the first sealing joint is communicated with the water inlet, and the second sealing joint is communicated with the water outlet; the solid-phase extraction module comprises an adsorption container, an adsorbent is arranged in the adsorption container, and the adsorption container is arranged between the first sealing joint and the second sealing joint; the thermotank covers the outer side of the solid-phase extraction module, and an oscillator is arranged at the bottom of the thermotank; and a power pump is arranged between the second sealing joint and the water outlet. According to the invention, a solid-phase extraction technology is advanced to a sample collection stage, so that the workload of on-site sampling can be greatly reduced, and the efficiency and accuracy of environmental monitoring and water quality analysis are improved.

Description

Technical Field

[0001] The invention relates to the technical field of environmental water sample monitoring, and in particular to an on-site water sample collection device and method based on solid phase extraction technology. Background Art

[0002] Solid Phase Extraction (SPE) is a commonly used sample pretreatment technology, widely used in medicine, food, environment and chemical industry. SPE is based on the theory of liquid-solid chromatography and uses selective adsorption and elution to separate and purify samples. By selecting suitable solid phase materials and operating conditions, the target substance can be separated from other impurities to achieve the purpose of enrichment and elution. SPE technology mainly includes two forms: solid phase extraction column and dispersed solid phase extractant. Solid phase extraction column (SPE) generally includes four steps: activation, loading, elution and elution; dispersed solid phase extractant (d-SPE) generally includes five steps: sample preparation, dispersion, enrichment, separation and elution.

[0003] Existing SPE technologies require the collection of large volumes of water samples and their delivery to the laboratory before sample pretreatment operations such as concentration, enrichment and purification are performed. In addition, in order to ensure the effectiveness of samples during collection, transportation and storage, it is often necessary to select corresponding sample preservatives and collection containers according to different monitoring targets. For unstable targets such as volatile organic compounds, there are even more stringent requirements for the storage environment (temperature, etc.) and shelf life. In actual sample collection, it is often necessary to collect samples in categories, which is highly professional and labor-intensive. Summary of the invention

[0004] Therefore, the technical problem to be solved by the present invention is to overcome the defects of large workload and low efficiency in the prior art of solid phase extraction of water samples.

[0005] In order to solve the above technical problems, the present invention provides an on-site water sample collection device based on solid phase extraction technology, comprising: A collection box, wherein a water inlet and a water outlet are arranged on the surface of the collection box; the water inlet is connected to a water inlet hose, and the water outlet is connected to a water outlet hose; a filter is arranged between the water inlet hose and the water inlet; A sampling component, wherein the sampling component is arranged inside the collection box, and the collection component includes a solid phase extraction module, a first sealing joint, a second sealing joint, a constant temperature box, an oscillator and a power pump, wherein the first sealing joint is connected to the water inlet, and the second sealing joint is connected to the water outlet; the solid phase extraction module includes an adsorption container, wherein an adsorbent is arranged in the adsorption container, and the two ends of the adsorption container are detachably connected to the first sealing joint and the second sealing joint respectively; the constant temperature box cover is arranged on the outside of the solid phase extraction module, the oscillator is arranged at the bottom of the constant temperature box, and the oscillator is abutted against the constant temperature box; the power pump is arranged between the second sealing joint and the water outlet.

[0006] In one embodiment of the present invention, a filter membrane is disposed in the filter, and the filter membrane is detachably connected to the filter.

[0007] In one embodiment of the present invention, a graduated sleeve is sleeved on one end of the water inlet hose away from the filter.

[0008] In one embodiment of the present invention, a flow meter is provided between the second sealing joint and the power pump.

[0009] In one embodiment of the present invention, a control component is also included, which includes a temperature controller, an oscillation frequency controller and a flow controller. The temperature controller is electrically connected to the constant temperature box, the oscillation frequency controller is electrically connected to the oscillator, and the flow controller is electrically connected to the flow meter and the power pump respectively.

[0010] In one embodiment of the present invention, the control component further comprises a time controller, and the time controller is electrically connected to the temperature controller, the oscillation frequency controller and the flow controller respectively.

[0011] In one embodiment of the present invention, a door, an electronic screen and a power switch are provided on the surface of the collection box, the door is arranged opposite to the constant temperature box, and the electronic screen and the power switch are electrically connected to the control component respectively.

[0012] A field water sample collection method based on solid phase extraction technology, using the field water sample collection device based on solid phase extraction technology to collect water samples, the specific operation steps are as follows: S1. Choose whether to install a filter and the filter membrane according to the water quality; S2. Connect the two ends of the empty adsorption container to the first sealing joint and the second sealing joint, place the water inlet hose in the target water body, set the cleaning parameters, and perform pipeline cleaning; S3. After cleaning, keep the water inlet hose in the target water body, select the corresponding adsorbent, replace the adsorption container with the adsorbent, set the sampling parameters, and start sampling; S4. After sampling is completed, the solid phase extraction module that has completed adsorption is disassembled, and both ends of the adsorption container are sealed, and the solid phase extraction module is reinstalled for the next sample collection; or the adsorbent that has completed collection is transferred and collected, and the adsorption container is reloaded with adsorbent for the next sample collection.

[0013] In one embodiment of the present invention, in step S2, the cleaning parameters are as follows: cleaning flow rate is 50-500 ml / min, cleaning time is 5-15 min, and cleaning temperature is 40-80°C.

[0014] In one embodiment of the present invention, in step S3, the adsorbent includes a solid phase extraction column and a dispersed solid phase extraction agent, wherein the sampling parameters of the solid phase extraction column are as follows: sampling flow rate is 0-100 ml / min, sampling time is 0-600 min, and sampling temperature is 0-80°C; the sampling parameters of the dispersed solid phase extraction agent are as follows: sampling flow rate is 0-100 ml / min, sampling time is 0-600 min, sampling temperature is 0-80°C, and sampling vibration frequency is 60-350 times / min.

[0015] The above technical solution of the present invention has the following advantages compared with the prior art: The present invention discloses an on-site water sample collection device and method based on solid phase extraction technology. The present invention moves the solid phase extraction technology forward to the sample collection stage, which can greatly reduce the workload of on-site sampling. After the target is enriched in the solid phase extraction material on-site, it will remain stable for a long time, which can effectively extend the shelf life and provide time and quality assurance for subsequent elution processing and detection analysis in the laboratory. The efficiency and accuracy of environmental monitoring and water quality analysis are improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to make the content of the present invention more clearly understood, the present invention is further described in detail below according to specific embodiments of the present invention in conjunction with the accompanying drawings, wherein Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a schematic diagram of the internal structure of the present invention; Figure 3 It is an operation flow chart of the present invention; Explanation of the reference numerals in the specification: 1. Collection box; 2. Sampling component; 3. Control component; 11. Water inlet; 12. Water outlet; 13. Water inlet hose; 14. Water outlet hose; 15. Filter; 16. Scale sleeve; 17. Box door; 18. Electronic screen; 19. Power switch; 21. Solid phase extraction module; 22. First sealing joint; 23. Second sealing joint; 24. Constant temperature box; 25. Oscillator; 26. Power pump; 27. Flow meter; 28. Mounting bracket; 31. Temperature controller; 32. Oscillation frequency controller; 33. Flow controller; 34. Time controller; 151. Filter membrane; 211. Adsorption container; 212. Adsorbent. DETAILED DESCRIPTION

[0017] The present invention is further described below in conjunction with the accompanying drawings and specific embodiments so that those skilled in the art can better understand the present invention and implement it, but the embodiments are not intended to limit the present invention. Embodiment 1

[0018] Reference Figure 1-Figure 3 As shown, the present invention discloses an on-site water sample collection device based on solid phase extraction technology, comprising: A collection box 1, wherein a water inlet 11 and a water outlet 12 are provided on the surface of the collection box 1; the water inlet 11 is connected to a water inlet hose 13, and the water outlet 12 is connected to a water outlet hose 14; a filter 15 is provided between the water inlet hose 13 and the water inlet 11; A sampling component 2, wherein the sampling component 2 is arranged inside the collection box 1, and the collection component includes a solid phase extraction module 21, a first sealing joint 22, a second sealing joint 23, a constant temperature box 24, an oscillator 25 and a power pump 26, wherein the first sealing joint 22 is connected to the water inlet 11, and the second sealing joint 23 is connected to the water outlet 12; the solid phase extraction module 21 includes an adsorption container 211, in which an adsorbent 212 is arranged, and the two ends of the adsorption container 211 are detachably connected to the first sealing joint 22 and the second sealing joint 23 respectively; the constant temperature box 24 is covered on the outside of the solid phase extraction module 21, and the oscillator 25 is arranged at the bottom of the constant temperature box 24, and the oscillator 25 is abutted against the constant temperature box 24; the power pump 26 is arranged between the second sealing joint 23 and the water outlet 12.

[0019] The collection box 1 in the present invention serves as the outer shell of the entire device, and the interior is used to install the sampling assembly 2. Specifically, a water inlet 11 and a water outlet 12 are provided on the surface of the collection box 1. The water inlet hose 13 at the water inlet 11 is used to introduce the water sample into the device, and the water outlet hose 14 at the water outlet 12 is used to discharge the collected water. In order to reduce the impact of impurities in the water sample on the test results and damage to the internal parts of the equipment, for water with high turbidity and high sediment content, a filter 15 needs to be installed at the water inlet 11 to filter out impurities in the water.

[0020] The solid phase extraction module 21 in the present invention includes an adsorption container 211 and an adsorbent 212. The solid phase extraction module 21 is detachably connected to the first sealing joint 22 and the second sealing joint 23. After the solid phase extraction module 21 is installed, the water sample is introduced into the solid phase extraction module 21 for use. Among them, the power pump 26 is used to provide power for the entire device, and the constant temperature box 24 is used to provide a certain temperature. The solid phase extraction module 21 is installed in the constant temperature box 24, which can improve the transfer efficiency of the target from the water body to the adsorbent 212, while maintaining a constant temperature, which helps to improve the reproducibility of water sample collection. As a preferred embodiment of the present invention, a mounting frame 28 is provided in the constant temperature box 24 for supporting the installation of the solid phase extraction module 21. Secondly, the oscillator 25 is arranged at the bottom of the constant temperature box 24. According to the selection of the adsorbent 212, when the dispersed solid phase extractant is used for sampling, the required oscillation frequency is set by the oscillator 25, which can improve the transfer efficiency of the target from the water body to the adsorbent 212.

[0021] The present invention moves the solid phase extraction technology forward to the sample collection stage, which can greatly reduce the workload of on-site sampling. After the target is enriched in the solid phase extraction material on-site, it will remain stable for a long time, which can effectively extend the shelf life and provide time and quality assurance for subsequent elution processing and detection analysis in the laboratory. It improves the efficiency and accuracy of environmental monitoring and water quality analysis.

[0022] Furthermore, a filter membrane 151 is disposed in the filter 15 , and the filter membrane 151 is detachably connected to the filter 15 .

[0023] In the present invention, the filter 15 can be installed according to the water quality. Similarly, different water qualities require corresponding different filter membranes 151 for filtering impurities of different diameters.

[0024] Specifically, the filter membrane 151 is classified according to the pore size, mainly including coarse filtration, microfiltration, ultrafiltration, nanofiltration and reverse osmosis. Environmental water sample collection mainly involves two types of coarse filtration and microfiltration. Coarse filtration is mainly used to remove large particles in the liquid, such as sediment, suspended matter and large biological particles (such as bacteria and algae). This type of filtration is usually used in the primary treatment stage to reduce the burden of subsequent treatment steps. The pore size of the filter membrane 151 is usually above 10μm. The microfiltration membrane 151 is mainly used to remove microorganisms and large particles, such as mycoplasma, yeast, etc. It is widely used in the biomedical field for sterilization and clarification of liquids. The pore size is generally between 0.1-10μm. Common ones are 0.45μm and 0.22μm.

[0025] Filter membrane 151 is classified by material, mainly including polymer filter membrane 151, ceramic filter membrane 151 and metal filter membrane 151. Common polymer filter membrane 151 are: Polyphenylene ether sulfone (PES) filter membrane 151: strong and durable, able to withstand long-term high-pressure filtration. It performs particularly well when a large number of samples are required. Mixed cellulose ester (MCE) filter membrane 151: It has strong water absorption, but may be easily blocked when dealing with turbid water samples. Therefore, it is more suitable for clearer water samples. Polycarbonate (PC) filter membrane 151: It is thin and easy to wrinkle, and may be blocked during use, so it is usually used for preventive filtration to prevent blockage. Polyvinylidene fluoride (PVDF) filter membrane 151: It has the lowest protein adsorption and good chemical compatibility, suitable for sterilization filtration and protein solution filtration. Nylon filter membrane 151: Both hydrophilic membrane and hydrophobic membrane are suitable for filtration of culture medium or antibody solution. Ceramic filter membrane 151 has good chemical stability and high temperature resistance. The metal filter membrane 151 has high mechanical strength and corrosion resistance.

[0026] Furthermore, one end of the water inlet hose 13 away from the filter 15 is sleeved with a graduated sleeve 16 .

[0027] Specifically, during the actual operation, water samples at a specific depth are collected through a casing with scale indication.

[0028] Furthermore, a flow meter 27 is provided between the second sealing joint 23 and the power pump 26 .

[0029] Specifically, the power pump 26 provides sampling power, and the real-time flow value is fed back through the flow meter 27 to determine the appropriate sampling flow rate. The sampling flow rate can be optimized and determined according to the adsorption efficiency of the adsorbent 212.

[0030] Furthermore, it also includes a control component 3, which includes a temperature controller 31, an oscillation frequency controller 32 and a flow controller 33. The temperature controller 31 is electrically connected to the constant temperature box 24, the oscillation frequency controller 32 is electrically connected to the oscillator 25, and the flow controller 33 is electrically connected to the flow meter 27 and the power pump 26 respectively.

[0031] Specifically, the temperature controller 31 is used to adjust the temperature in the thermostat 24, the oscillation frequency controller 32 is used to adjust the vibration frequency of the oscillator 25, and the flow controller 33 is electrically connected to the water pump and the flow meter 27 respectively. The flow meter 27 feeds back the detected real-time flow value to the flow controller 33, and the flow controller 33 controls the water pump to change the flow size. In the actual operation process, the sampling flow rate can be optimized and determined according to the adsorption efficiency of the adsorbent 212.

[0032] Furthermore, the control component 3 also includes a time controller 34, and the time controller 34 is electrically connected to the temperature controller 31, the oscillation frequency controller 32 and the flow controller 33 respectively.

[0033] Specifically, the time controller 34 is electrically connected to the temperature controller 31 , the oscillation frequency controller 32 and the flow controller 33 , and can control the working time of the constant temperature box 24 , the vibration time of the oscillator 25 , and the operation time of the flow controller 33 .

[0034] Furthermore, a door 17 , an electronic screen 18 and a power switch 19 are provided on the surface of the collection box 1 , the door 17 is arranged opposite to the constant temperature box 24 , and the electronic screen 18 and the power switch 19 are electrically connected to the control component 3 respectively.

[0035] Specifically, the power switch 19 is used to start the entire device, the electronic screen 18 can be used to feedback various parameters, and can also control the control component 3 to adjust the parameter settings. The box door 17 is used to seal the collection box 1, open the box door 17, and install the solid phase extraction module 21. Embodiment 2

[0036] Reference Figure 3 As shown, a field water sample collection method based on solid phase extraction technology is used to collect water samples using the field water sample collection device based on solid phase extraction technology described in Example 1. The specific operation steps are as follows: S1. Select whether to install the filter 15 and the filter membrane 151 in the filter 15 according to the water quality; S2, connecting the two ends of the empty adsorption container 211 to the first sealing joint 22 and the second sealing joint 23, placing the water inlet hose 13 in the target water body, setting the cleaning parameters, and performing pipeline cleaning; S3, after the cleaning is completed, the water inlet hose 13 is kept in the target water body, the corresponding adsorbent 212 is selected, the adsorption container 211 loaded with the adsorbent 212 is replaced, the sampling parameters are set, and the sampling is started; S4. After sampling is completed, the solid phase extraction module 21 that has completed adsorption is disassembled, and both ends of the adsorption container 211 are sealed, and the solid phase extraction module 21 is reinstalled for the next sample collection; or the adsorbent 212 that has completed collection is transferred and collected, and the adsorption container 211 is reloaded with the adsorbent 212 for the next sample collection.

[0037] Specifically, in step S1, before sampling, the solid phase extraction module 21 (SPE) is determined to be pre-activated according to the type of adsorbent 212 and the characteristics of the monitored target; upon arriving at the sampling site, it is determined whether to install the filter 15 and to select the filter membrane 151 with a suitable pore size according to the water quality; In step S2, the box door 17 is opened, and the empty adsorbent 212 container is connected to two sealing joints, where the diameters of the first sealing joint 22 and the second sealing joint 23 and the connection port of the solid phase extraction module 21 are adjustable to adapt to adsorbent 212 containers of different specifications; then the box door 17 is closed, the power switch 19 is turned on, and the electronic screen 18 waits for the self-test to be displayed on the self-test pass, and the custom parameters in the cleaning mode are set; the water inlet hose 13 is placed in the target water body, and the scale sleeve 16 is installed at the end of the hose. According to the scale line indication, water samples of a specific depth are collected. Before formal collection, the cleaning mode is used to clean the pipeline to ensure that the collected water samples are not contaminated by the background of the sampling system; specifically, the cleaning parameters are as follows: the cleaning flow rate is 50-500ml / min, the cleaning time is 5-15min, and the cleaning temperature is 40-80℃.

[0038] In the above data, the cleaning time is based on the water outlet time of the water outlet hose 14. If the cleaning time is less than 5 minutes, it is difficult to clean the inside of the pipeline. It is advisable to ensure that the cleaning time is more than 5 minutes; the cleaning stage before the start of sampling is turned on according to the temperature required by the sampling mode to balance the system temperature; the system cleaning after the sampling is completed can set a higher temperature (40-80°C) to remove residual pollutants in the system, and ensure a certain flow rate to carry away the residues through the water flow. It should be noted that during the cleaning process, the oscillator 25 can be turned on according to the on-site conditions to improve the cleaning effect and cleaning efficiency. Preferably, the cleaning flow rate is 200ml / min. The cleaning time is 8 minutes, and the cleaning temperature is preferably 60°C. The parameter settings should be appropriately changed on-site according to the sampling environment and the amount of residual pollutants in the equipment.

[0039] In step S3, after cleaning, keep the water inlet hose 13 in the target water body, open the box door 17, replace the adsorbent 212 container with the adsorbent 212, and close the box door 17; set the sampling parameters in the sampling mode through the electronic screen 18, and start sampling; in this step, it is necessary to determine whether to set the vibration parameters according to the type of adsorbent 212. The adsorbent 212 includes a solid phase extraction column and a dispersed solid phase extraction agent, wherein the sampling parameters of the solid phase extraction column are as follows: the sampling flow rate is 0-100ml / min, the sampling time is 0-600min, and the sampling temperature is 0-80℃; the sampling parameters of the dispersed solid phase extraction agent are as follows: the sampling flow rate is 0-100ml / min, the sampling time is 0-600min, and the sampling temperature is 0-80℃; when using a dispersed solid phase extraction agent, in order to make the dispersed solid phase extraction agent fully contact with the flowing water body, set the necessary vibration frequency to ensure that the adsorbent 212 remains suspended in the adsorbent 212 container; preferably, the sampling vibration frequency is 60-350 times / min.

[0040] Among the above parameters, the adjustment of temperature needs to comprehensively consider the effect of temperature on molecular motion, volatility, solubility and distribution coefficient, and determine the optimal extraction temperature through experiments to balance the above positive and negative effects and achieve efficient and accurate analysis results. Although high temperature can accelerate molecular motion, too high temperature will bring inconvenience to operation, so the extraction temperature generally does not exceed 80℃.

[0041] In step S4, after the sampling is completed, the solid phase extraction module 21 is removed, the two ends of the adsorption container 211 are sealed with matching lids, and the solid phase extraction module 21 is reinstalled for the next sample collection, and step S3 is repeated until the water sample collection is completed. In addition, the adsorbent 212 that has been collected can be transferred and collected, and the adsorbent 212 container can be reloaded with the adsorbent 212 for the next sample collection. Finally, the solid phase extraction module 21 that adsorbs the target object is sent to the laboratory for the next experimental analysis. Embodiment 3

[0042] For the collection of water samples for monitoring pesticide residues in drinking water plants: Targets: Dichlorvos, 2,4,6-trichlorophenol, hexachlorobenzene, dimethoate, pentachlorophenol, lindane, chlorothalonil, methyl parathion, heptachlor, malathion, chlorpyrifos, parathion, DDT, deltamethrin, etc. Solid phase extraction column type: Oasis HLB, Bond Elut-PPL, etc. Solid phase extraction column specifications: 200mg / 6mL; Filter 15: The factory water is clean water and can be filtered by 0.45μm glass fiber filter membrane 151 or without filter 15; Sampling flow rate: 20mL / min; Sampling time: 50min, sampling volume 1L; Temperature: Set a fixed temperature within the range of (20-40)°C, such as 25°C, to offset the temperature differences between different batches of samples and improve sampling repeatability; Vibration frequency: Off; Subsequent laboratory testing and analysis methods: liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry; The adsorbent in this embodiment adopts a solid phase extraction column to achieve on-site sampling. Compared with the sampling in the prior art, the water sample for pesticide pollutant detection needs to be collected in a ground hard brown glass bottle of 2.5L, and about 100mg of ascorbic acid is added to each liter of water sample, mixed and shaken to remove residual chlorine, and the sample bottle is sealed and stored in a refrigerator at 0~4℃. The sample storage time is 24h. The operation of this embodiment is simple, and the sample can be stored for a long time. The solid phase extraction column after adsorbing the water sample can be stored at room temperature for 7-10d, and the low temperature storage time below 0℃ is longer. Embodiment 4

[0043] Regarding the collection of water samples for monitoring of halogenated quinone compounds in surface water: Target substances: 2-chloro-5-methyl-1,4-benzoquinone (MCBQ), 2-bromo-1,4-benzoquinone (BBQ), 2,5-dibromo-1,4-benzoquinone (DBBQ), tetrachloro-p-benzoquinone (TCBQ), tetrabromo-p-benzoquinone (TBBQ); Dispersed solid phase extractant: EB-COF:Br material; Dispersed solid phase extractant loading: 30 mg; Filter 15: Select coarse filtration, microfiltration or coarse filtration + microfiltration according to the cleanliness of the water sample; Sampling flow rate: 5mL / min; Sampling time: 20min, sampling volume 100mL; Temperature: Set a fixed temperature within the range of (20-40)°C, such as 25°C, to offset the temperature differences between different batches of samples and improve sampling repeatability; Vibration frequency: 100 times / min; Vibration time: 20 min (same as sample loading time); Subsequent laboratory testing and analysis methods: liquid chromatography-mass spectrometry technology.

[0044] The adsorbent in this embodiment adopts a dispersed solid phase extractant to achieve on-site sampling. Compared with the sampling in the prior art, the water sample for the detection of halogenated quinone disinfection byproducts needs to be collected with a ground hard brown glass bottle of 1L, and 2.5mL of formic acid preservative is added immediately after collection, mixed and shaken to terminate the reaction of the target and residual chlorine, and the acidity of the water sample is adjusted to stabilize the test object, the sample bottle is sealed, and refrigerated at 0~4℃. The sample storage time is 5d. If no preservative is added, the water sample storage time is less than 24h. The operation of this embodiment is simple, and the sample can be stored for a long time. The solid phase extractant after adsorbing the water sample can be stored at room temperature for 7-10d, and the low temperature storage time below 0℃ is longer.

[0045] In summary, the present invention introduces an on-site water sample collection device and method based on solid phase extraction technology. The present invention moves the solid phase extraction technology forward to the sample collection stage, which can greatly reduce the workload of on-site sampling. After the target object is enriched in the solid phase extraction material on-site, it will remain stable for a long time, which can effectively extend the shelf life, provide time and quality assurance for subsequent elution processing and detection analysis in the laboratory, and improve the efficiency and accuracy of environmental monitoring and water quality analysis.

[0046] Obviously, the above embodiments are merely examples for the purpose of clear explanation and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the present invention.

Claims

1. A field water sample collection device based on solid phase extraction technology, characterized in that: include: A collection box, wherein a water inlet and a water outlet are arranged on the surface of the collection box; the water inlet is connected to a water inlet hose, and the water outlet is connected to a water outlet hose; a filter is arranged between the water inlet hose and the water inlet; A sampling component, wherein the sampling component is arranged inside the collection box, and the collection component includes a solid phase extraction module, a first sealing joint, a second sealing joint, a constant temperature box, an oscillator and a power pump, wherein the first sealing joint is connected to the water inlet, and the second sealing joint is connected to the water outlet; the solid phase extraction module includes an adsorption container, wherein an adsorbent is arranged in the adsorption container, and the two ends of the adsorption container are detachably connected to the first sealing joint and the second sealing joint respectively; the constant temperature box cover is arranged on the outside of the solid phase extraction module, the oscillator is arranged at the bottom of the constant temperature box, and the oscillator is abutted against the constant temperature box; the power pump is arranged between the second sealing joint and the water outlet.

2. The on-site water sample collection device based on solid phase extraction technology according to claim 1, characterized in that: A filter membrane is arranged in the filter, and the filter membrane is detachably connected to the filter.

3. The on-site water sample collection device based on solid phase extraction technology according to claim 1, characterized in that: One end of the water inlet hose away from the filter is sleeved with a graduated sleeve.

4. The on-site water sample collection device based on solid phase extraction technology according to claim 1, characterized in that: A flow meter is arranged between the second sealing joint and the power pump.

5. The on-site water sample collection device based on solid phase extraction technology according to claim 4 is characterized in that: It also includes a control component, which includes a temperature controller, an oscillation frequency controller and a flow controller. The temperature controller is electrically connected to the constant temperature box, the oscillation frequency controller is electrically connected to the oscillator, and the flow controller is electrically connected to the flow meter and the power pump respectively.

6. The on-site water sample collection device based on solid phase extraction technology according to claim 5, characterized in that: The control component also includes a time controller, which is electrically connected to the temperature controller, the oscillation frequency controller and the flow controller respectively.

7. The on-site water sample collection device based on solid phase extraction technology according to claim 5, characterized in that: The surface of the collection box is provided with a box door, an electronic screen and a power switch, the box door is arranged opposite to the constant temperature box, and the electronic screen and the power switch are electrically connected to the control component respectively.

8. A method for collecting water samples on site based on solid phase extraction technology, using a device for collecting water samples on site based on solid phase extraction technology as claimed in any one of claims 1 to 7, characterized in that: The specific steps are as follows: S1. Choose whether to install a filter and the filter membrane according to the water quality; S2. Connect the two ends of the empty adsorption container to the first sealing joint and the second sealing joint, place the water inlet hose in the target water body, set the cleaning parameters, and perform pipeline cleaning; S3. After cleaning, keep the water inlet hose in the target water body, select the corresponding adsorbent, replace the adsorption container with the adsorbent, set the sampling parameters, and start sampling; S4. After sampling is completed, the solid phase extraction module that has completed adsorption is disassembled, and both ends of the adsorption container are sealed, and the solid phase extraction module is reinstalled for the next sample collection; or the adsorbent that has completed collection is transferred and collected, and the adsorption container is reloaded with adsorbent for the next sample collection.

9. The on-site water sample collection method based on solid phase extraction technology according to claim 8, characterized in that: In step S2, the cleaning parameters are as follows: cleaning flow rate is 50-500 ml / min, cleaning time is 5-15 min, and cleaning temperature is 40-80°C.

10. The on-site water sample collection method based on solid phase extraction technology according to claim 8, characterized in that: In step S3, the adsorbent includes a solid phase extraction column and a dispersed solid phase extraction agent, wherein the sampling parameters of the solid phase extraction column are as follows: sampling flow rate is 0-100 ml / min, sampling time is 0-600 min, and sampling temperature is 0-80°C; the sampling parameters of the dispersed solid phase extraction agent are as follows: sampling flow rate is 0-100 ml / min, sampling time is 0-600 min, sampling temperature is 0-80°C, and sampling vibration frequency is 60-350 times / min.

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