Composition with helicobacter pylori resisting effect and preparation method thereof

Abstract

The invention relates to the technical field of food, and particularly discloses a composition with an anti-helicobacter pylori effect and a preparation method thereof. The preparation method of the composition with the helicobacter pylori resisting effect comprises the following steps: step 1) putting dendrobium officinale, semen raphani, citron and lindera aggregata leaves into water, heating and extracting to obtain a pre-extract; step 2), adding rose kocuria, fermentation lactobacillus mucilaginosus and lactococcus raffinosus into the pre-extract for fermentation to obtain a fermented product; the composition with the helicobacter pylori resisting effect is prepared by the following steps of (1) preparing fermentation broth, (2) preparing fermentation broth, (3) filtering and degerming the fermentation broth to obtain fermentation broth, (4) concentrating and drying the fermentation broth to obtain fermentation powder, and (5) adding lactic acid bacteria powder and fucoidin into the fermentation powder, and uniformly mixing to obtain the composition with the helicobacter pylori resisting effect The composition has the advantage of improving the effect of removing helicobacter pylori in intestines and stomach.

Description

Technical Field

[0001] The invention relates to the field of food, and in particular to a composition with anti-Helicobacter pylori efficacy and a preparation method thereof. Background Art

[0002] Helicobacter pylori is a globally prevalent bacterium that has been found in the stomachs of patients around the world. Helicobacter pylori can cause symptoms such as abdominal pain, bloating, nausea, acid reflux, and indigestion, which seriously affect their quality of life and health.

[0003] In order to eliminate Helicobacter pylori in the stomach and intestines, bactericidal drugs are usually used. Although taking bactericidal drugs can eliminate Helicobacter pylori, the side effects of the drugs are large and will cause significant discomfort to the stomach and intestines. Therefore, consumers currently prefer to use food therapy to eliminate Helicobacter pylori, by eating foods that have the ability to kill and absorb Helicobacter pylori to achieve the effect of eliminating Helicobacter pylori in the stomach and intestines.

[0004] However, there are currently few types of foods on the market that are effective against Helicobacter pylori, and their effectiveness is relatively slow, making it difficult to meet the needs of consumers. Therefore, there is still room for improvement. Summary of the invention

[0005] In order to improve the effect of clearing Helicobacter pylori in the stomach and intestines, the present application provides a composition with anti-Helicobacter pylori efficacy and a preparation method thereof.

[0006] In a first aspect, the present application provides a method for preparing a composition having anti-Helicobacter pylori efficacy, using the following technical solution: A method for preparing a composition having anti-Helicobacter pylori efficacy comprises the following steps: Step 1), putting Dendrobium officinale, Radish Seed, Citron, and Linderae umbellatae Leaves into water, heating and extracting, to obtain a pre-extract; Step 2), adding Coxiella rosea, Lactobacillus mucilaginosus fermentation, and Lactococcus raffinose to the pre-extract for fermentation to obtain a fermentation product; Step 3), filtering and sterilizing the fermented product to obtain a fermentation liquid; Step 4), concentrating and drying the fermentation liquid to obtain baking powder; Step 5), lactic acid bacteria powder and fucoidan are added to the fermentation powder, and mixed evenly to obtain a composition with anti-Helicobacter pylori efficacy.

[0007] By adopting the above technical scheme, by compounding Dendrobium officinale, Radish Seed, Citron, and Linderae umbellata Leaves and then fermenting them with Coxiella rosea, Lactobacillus mucilaginosus, and Lactococcus raffinose, the active ingredients in the obtained fermentation powder and the fermentation products cooperate with each other, which can have a significant effect of killing Helicobacter pylori. When combined with lactic acid bacteria powder and fucoidan, it can well promote the excretion of Helicobacter pylori, making the effect of eliminating Helicobacter pylori more significant, and can well regulate the stomach and intestines, so that the stomach and intestines can quickly restore health, quickly relieve gastrointestinal discomfort caused by Helicobacter pylori infection, and well meet the needs of consumers.

[0008] Preferably, the mass ratio of the Dendrobium officinale, Radish Seed, Citron, and Linderae umbellatae Leaves is 10-15:10-15:10-15:10-15.

[0009] By adopting the above technical scheme and specifically selecting the mass ratio of Dendrobium officinale, Radish Seed, Citron and Linderae umbellatae Leaves, the various active ingredients can cooperate with each other more effectively, thereby achieving a better effect in eliminating Helicobacter pylori.

[0010] Preferably, the mass ratio of Coxiella rosea, Lactobacillus mucilaginosus and Lactococcus raffinose is 0.05-0.07:0.03-0.05:0.02-0.04.

[0011] By adopting the above technical scheme and specifically selecting the mass ratio of Coxiella rosea, Lactobacillus mucilaginosus and Lactococcus raffinose, the fermentation effect is better, and the fermentation product is better matched with the active ingredient, thereby achieving the effect of eliminating Helicobacter pylori.

[0012] Preferably, the mass ratio of the Dendrobium officinale, Radish Seed, Citron, Linderae umbellatae Leaves, Coxiella rosea, Lactobacillus mucilaginosus and Lactococcus raffinose is 10-15:10-15:10-15:10-15:0.05-0.07:0.03-0.05:0.02-0.04.

[0013] By adopting the above technical scheme, by specifically selecting the mass ratio of Dendrobium officinale, Radish Seed, Citron, Linderae umbellatae Leaves, Coxiella rosea, Lactobacillus mucilaginosus fermentation, and Lactococcus raffinose, the active ingredients and the fermentation products are better matched in a specific ratio, and a more significant effect of eliminating Helicobacter pylori is exerted, which can eliminate Helicobacter pylori in the stomach and intestines more quickly.

[0014] Preferably, the mass ratio of the Dendrobium officinale, Radish Seed, Citron, Linderae umbellatae Leaves and water is 10-15:10-15:10-15:10-15:200-300.

[0015] By adopting the above technical scheme and specifically selecting the mass ratio of Dendrobium officinale, Radish Seed, Citron, Linderae umbellatae Leaves and water, the extraction effect is better, the active ingredients are more fully extracted, the raw material utilization rate is higher, and the economic value is higher.

[0016] Preferably, in step 5), the mass ratio of baking powder, lactic acid bacteria powder and fucoidan is 40-60:15-25:25-35.

[0017] By adopting the above technical scheme and specifically selecting the mass ratio of baking powder, lactic acid bacteria powder and fucoidan, the stomach and intestines can be repaired better and faster while Helicobacter pylori is quickly eliminated, gastrointestinal discomfort can be quickly relieved, and the effect of regulating the stomach and intestines is better.

[0018] Preferably, in step 1), during the heating extraction, the mixture is heated to boiling and the extraction is performed for 1-2 hours.

[0019] By adopting the above technical solution, the active ingredients in each raw material can be more easily extracted, and the quality of the prepared composition with anti-Helicobacter pylori efficacy is better.

[0020] Preferably, in step 2), during fermentation, the temperature is kept at 30-32° C. and the fermentation lasts for 18-20 days.

[0021] By adopting the above technical solution, the activity of each bacteria is higher, the fermentation effect is better, and the composition with anti-Helicobacter pylori efficacy has a better effect in eliminating Helicobacter pylori.

[0022] In a second aspect, the present application provides a composition having anti-Helicobacter pylori efficacy, which adopts the following technical solution: A composition with anti-Helicobacter pylori efficacy is prepared by the above-mentioned preparation method of the composition with anti-Helicobacter pylori efficacy.

[0023] By adopting the above technical scheme, the composition with anti-Helicobacter pylori efficacy can quickly eliminate Helicobacter pylori in the stomach and intestines, and has a good effect of repairing the stomach and intestines, thus meeting the needs of consumers well.

[0024] In summary, this application has the following beneficial effects: 1. Since the present application adopts Dendrobium officinale, Radish Seed, Citron, and Linderae umbellatae leaves to compound and then ferment with Coxiella rosea, Lactobacillus mucilaginosus, and Lactococcus raffinose, the active ingredients in the obtained fermentation powder cooperate with the fermentation products to have a significant effect of killing Helicobacter pylori. When combined with lactic acid bacteria powder and fucoidan, it can well promote the excretion of Helicobacter pylori, making the effect of eliminating Helicobacter pylori more significant, and can well regulate the stomach and intestines, so that the stomach and intestines can quickly restore health, quickly relieve the gastrointestinal discomfort caused by Helicobacter pylori infection, and well meet the needs of consumers.

[0025] 2. In the present application, it is preferred to specifically select the mass ratio of Dendrobium officinale, Radish Seed, Citron, Linderae umbellatae Leaves, Coxiella rosea, Lactobacillus mucilaginosus fermentation, and Lactococcus raffinose so that the active ingredients and the fermentation products are better matched in a specific ratio, so as to exert a more significant effect of eliminating Helicobacter pylori and eliminate Helicobacter pylori in the stomach and intestines faster.

[0026] 3. In the present application, it is preferred to specifically select the mass ratio of baking powder, lactic acid bacteria powder and fucoidan, so that the stomach and intestines can be repaired better and faster while Helicobacter pylori is quickly eliminated, gastrointestinal discomfort can be quickly relieved, and the effect of regulating the stomach and intestines is better. DETAILED DESCRIPTION

[0027] The present application is further described in detail below with reference to the embodiments.

[0028] Example 1 A method for preparing a composition having anti-Helicobacter pylori efficacy comprises the following steps: Step 1), put 10kg of Dendrobium officinale, 10kg of Radish Seed, 10kg of Citron, and 10kg of Linderae umbellatae Leaves into 200kg of water, heat to boiling, condense and reflux, extract for 1h, and obtain a pre-extract.

[0029] Step 2), cooling the pre-extract to room temperature, then adding 0.05 kg of Coxiella rosea, 0.03 kg of fermented Lactobacillus mucilaginosus, and 0.02 kg of Lactococcus raffinose to the pre-extract, keeping the temperature at 30° C., and fermenting for 18 days to obtain a fermentation product.

[0030] Step 3), the fermented product is filtered through a filter cloth, and the filtrate is taken, and the filtrate is filtered through a 0.22 μm filter membrane for sterilization to obtain a fermentation broth.

[0031] Step 4), the fermentation liquid is concentrated by rotary evaporation at a rotary evaporation temperature of 60° C. and a rotary evaporation pressure of 0.01 MPa, and concentrated to a paste, and then the paste is sprayed with powder and dried to obtain baking powder.

[0032] Step 5), put 40g baking powder, 25g lactic acid bacteria powder and 35g fucoidan into a stirring kettle, rotate at 40r / min, stir for 30min, mix evenly, and obtain a composition with anti-Helicobacter pylori efficacy.

[0033] Dendrobium officinale is from commercially available, dry product.

[0034] Radish seeds are from commercially available, dried products.

[0035] Citron is from commercially available, dry product.

[0036] Linderae umbellatae leaves are from commercial sources and are dried products.

[0037] Coxiella rosea was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-82699.

[0038] Fermented Lactobacillus mucilaginosus was purchased from Beijing Biobo Biotechnology Co., Ltd., platform number: Bio-84997.

[0039] Lactococcus raffinose was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-74847.

[0040] The lactic acid bacteria powder was purchased from Shandong Gushuo Biotechnology Co., Ltd.

[0041] Fucoidan was purchased from Shaanxi Chenming Biotechnology Co., Ltd.

[0042] Example 2 A method for preparing a composition having anti-Helicobacter pylori efficacy comprises the following steps: Step 1), put 12.5kg of Dendrobium officinale, 12.5kg of Radish Seed, 12.5kg of Citron, and 12.5kg of Linderae umbellatae Leaves into 250kg of water, heat to boiling, condense and reflux, extract for 1.5h, and obtain a pre-extract.

[0043] Step 2), cooling the pre-extract to room temperature, then adding 0.06 kg of Coxiella rosea, 0.04 kg of fermented Lactobacillus mucilaginosus, and 0.03 kg of Lactococcus raffinose to the pre-extract, keeping the temperature at 31° C., and fermenting for 19 days to obtain a fermentation product.

[0044] Step 3), the fermented product is filtered through a filter cloth, and the filtrate is taken, and the filtrate is filtered through a 0.22 μm filter membrane for sterilization to obtain a fermentation broth.

[0045] Step 4), the fermentation liquid is concentrated by rotary evaporation at a rotary evaporation temperature of 60° C. and a rotary evaporation pressure of 0.01 MPa, and concentrated to a paste, and then the paste is sprayed with powder and dried to obtain baking powder.

[0046] Step 5), put 50g baking powder, 20g lactic acid bacteria powder and 30g fucoidan into a stirring kettle, rotate at 40r / min, stir for 30min, mix evenly, and obtain a composition with anti-Helicobacter pylori efficacy.

[0047] Dendrobium officinale is from commercially available, dry product.

[0048] Radish seeds are from commercially available, dried products.

[0049] Citron is from commercially available, dry product.

[0050] Linderae umbellatae leaves are from commercial sources and are dried products.

[0051] Coxiella rosea was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-82699.

[0052] Fermented Lactobacillus mucilaginosus was purchased from Beijing Biobo Biotechnology Co., Ltd., platform number: Bio-84997.

[0053] Lactococcus raffinose was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-74847.

[0054] The lactic acid bacteria powder was purchased from Shandong Gushuo Biotechnology Co., Ltd.

[0055] Fucoidan was purchased from Shaanxi Chenming Biotechnology Co., Ltd.

[0056] Example 3 A method for preparing a composition having anti-Helicobacter pylori efficacy comprises the following steps: Step 1), put 15kg of Dendrobium officinale, 15kg of Radish Seed, 15kg of Citron, and 15kg of Linderae umbellatae Leaves into 300kg of water, heat to boiling, condense and reflux, extract for 2h, and obtain a pre-extract.

[0057] Step 2), cooling the pre-extract to room temperature, then adding 0.07 kg of Coxiella rosea, 0.05 kg of fermented Lactobacillus mucilaginosus, and 0.04 kg of Lactococcus raffinose to the pre-extract, keeping the temperature at 32° C., and fermenting for 20 days to obtain a fermentation product.

[0058] Step 3), the fermented product is filtered through a filter cloth, and the filtrate is taken, and the filtrate is filtered through a 0.22 μm filter membrane for sterilization to obtain a fermentation broth.

[0059] Step 4), the fermentation liquid is concentrated by rotary evaporation at a rotary evaporation temperature of 60° C. and a rotary evaporation pressure of 0.01 MPa, and concentrated to a paste, and then the paste is sprayed with powder and dried to obtain baking powder.

[0060] Step 5), put 60g baking powder, 15g lactic acid bacteria powder and 25g fucoidan into a stirring kettle, rotate at 40r / min, stir for 30min, mix evenly, and obtain a composition with anti-Helicobacter pylori efficacy.

[0061] Dendrobium officinale is from commercially available, dry product.

[0062] Radish seeds are from commercially available, dried products.

[0063] Citron is from commercially available, dry product.

[0064] Linderae umbellatae leaves are from commercial sources and are dried products.

[0065] Coxiella rosea was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-82699.

[0066] Fermented Lactobacillus mucilaginosus was purchased from Beijing Biobo Biotechnology Co., Ltd., platform number: Bio-84997.

[0067] Lactococcus raffinose was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-74847.

[0068] The lactic acid bacteria powder was purchased from Shandong Gushuo Biotechnology Co., Ltd.

[0069] Fucoidan was purchased from Shaanxi Chenming Biotechnology Co., Ltd.

[0070] Comparative Example 1 A method for preparing a composition having anti-Helicobacter pylori efficacy, compared with Example 2, the only difference is: Use purslane to replace Dendrobium officinale in equal amounts.

[0071] Purslane is from commercially available, dry product.

[0072] Comparative Example 2 A method for preparing a composition having anti-Helicobacter pylori efficacy, compared with Example 2, the only difference is: Replace radish seeds with an equal amount of perilla.

[0073] Perilla is from commercially available, dry product.

[0074] Comparative Example 3 A method for preparing a composition having anti-Helicobacter pylori efficacy, compared with Example 2, the only difference is: Replace citron with an equal amount of licorice.

[0075] Licorice root is from commercially available, dry product.

[0076] Comparative Example 4 A method for preparing a composition having anti-Helicobacter pylori efficacy, compared with Example 2, the only difference is: Replace the Linderae umbellatae leaves with an equal amount of dandelion.

[0077] Dandelion is from commercially available, dried product.

[0078] Comparative Example 5 A method for preparing a composition having anti-Helicobacter pylori efficacy, compared with Example 2, the only difference is: Bacillus palmitofermentans was used to replace Coxiella rosea in equal amounts.

[0079] Bacillus palmitolyticus was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-00034.

[0080] Comparative Example 6 A method for preparing a composition having anti-Helicobacter pylori efficacy, compared with Example 2, the only difference is: The fermented amino acid coccus was used in equal amounts to replace the fermented mucus lactobacillus.

[0081] The fermentative amino acid coccus was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-80023.

[0082] Comparative Example 7 A method for preparing a composition having anti-Helicobacter pylori efficacy, compared with Example 2, the only difference is: Lactococcus raffinose was replaced by Bacillus fermentans in equal amounts.

[0083] Fermented Cellulomonas was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-53149.

[0084] Experiment 1 Volunteers infected with Helicobacter pylori were recruited into groups of ten. Blood was drawn from the volunteers to test their Helicobacter pylori antibodies (HP), and the average value was taken and recorded as the initial Helicobacter pylori antibody (HP).

[0085] The volunteers were divided into groups and took the composition with anti-Helicobacter pylori efficacy of each embodiment and comparative example for 14 consecutive days, three times a day, 5 g each time. After taking it for 14 days, blood was drawn again to detect the Helicobacter pylori antibody (HP) of the volunteers, and the average was taken and recorded as the conditioned Helicobacter pylori antibody (HP).

[0086] Experiment 2 A number of MRS culture media were prepared. When preparing the culture media, the composition with anti-Helicobacter pylori efficacy of each embodiment and comparative example was added at a ratio of 1 g of the composition with anti-Helicobacter pylori efficacy / 100 g of the culture media. Nutrients were injected into the culture dishes to prepare a number of MRS culture media containing the composition with anti-Helicobacter pylori efficacy. Ten culture media formed a group, and each culture medium was inoculated with 10 cuf of lactic acid bacteria, which were commercially available and had the strain number: ATCC 8008.

[0087] After inoculation, culture the culture medium at a constant temperature of 37°C for 24 hours in a sterile environment, and observe and record the number of lactic acid bacteria colonies in the culture medium.

[0088] The specific experimental data of Experiment 1-2 are shown in Table 1.

[0089] Table 1 According to the data comparison of each embodiment and the comparative example in Table 1, it can be obtained that the effect of each embodiment in eliminating Helicobacter pylori is significantly better than that of each comparative example, and the effect of each embodiment in promoting the growth of lactic acid bacteria is significantly better than that of each comparative example, and the gastrointestinal tract can be better and faster regulated, so that the gastrointestinal tract can be restored to health, and the gastrointestinal discomfort caused by Helicobacter pylori infection can be effectively eliminated.

[0090] Each comparative example uses raw materials that can eliminate Helicobacter pylori, but each comparative example destroys the special combination of Dendrobium officinale, Radish Seed, Citron, and Linderae umbellatae Leaves, as well as the special fermentation system of Coxiella rosea, Lactobacillus mucilaginosus, and Lactococcus raffinose, resulting in the active ingredients and fermentation products being unable to produce significant Helicobacter pylori elimination effects after cooperating with each other. As a result, after taking the compositions of each comparative example, although Helicobacter pylori can be reduced to a certain extent, the speed of reducing Helicobacter pylori is slow, which is difficult to meet the needs of consumers.

[0091] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed. However, as long as it is within the scope of the claims of the present application, it shall be protected by the patent law.

Claims

1. A method for preparing a composition having anti-Helicobacter pylori efficacy, characterized in that: The following steps are involved: Step 1), putting Dendrobium officinale, Radish seeds, Citron, and Linderae umbellatae leaves into water, heating and extracting, to obtain a pre-extract; Step 2), adding Coxiella rosea, Lactobacillus fermentans, and Lactococcus raffinose to the pre-extract for fermentation to obtain a fermentation product; Step 3), filtering and sterilizing the fermented product to obtain a fermentation liquid; Step 4), concentrating and drying the fermentation liquid to obtain baking powder; Step 5), lactic acid bacteria powder and fucoidan are added to the fermentation powder and mixed evenly to obtain a composition with anti-Helicobacter pylori efficacy.

2. The method for preparing a composition having anti-Helicobacter pylori efficacy according to claim 1, characterized in that: The mass ratio of the dendrobium officinale, radish seeds, citron and lindera umbellatae leaves is 10-15:10-15:10-15:10-15.

3. The method for preparing a composition having anti-Helicobacter pylori efficacy according to claim 1, characterized in that: The mass ratio of Coxiella rosea, Lactobacillus mucilaginosus and Lactococcus raffinose is 0.05-0.07: 0.03-0.05: 0.02-0.

04.

4. The method for preparing a composition having anti-Helicobacter pylori efficacy according to claim 1, characterized in that: The mass ratio of the dendrobium officinale, radish seeds, citron, lindera serrata leaves, Coxiella rosea, fermented mucus lactobacillus and raffinose lactococcus is 10-15:10-15:10-15:10-15:0.05-0.07:0.03-0.05:0.02-0.

04.

5. The method for preparing a composition having anti-Helicobacter pylori efficacy according to claim 4, characterized in that: The mass ratio of the dendrobium officinale, radish seeds, citron, lindera umbellatae leaves and water is 10-15:10-15:10-15:10-15:200-300.

6. The method for preparing a composition having anti-Helicobacter pylori efficacy according to claim 5, characterized in that: In the step 5), the mass ratio of baking powder, lactic acid bacteria powder and fucoidan is 40-60:15-25:25-35.

7. The method for preparing a composition having anti-Helicobacter pylori efficacy according to claim 1, characterized in that: In the step 1), when heating for extraction, the mixture is heated to boiling and the extraction is performed for 1-2 hours.

8. The method for preparing a composition having anti-Helicobacter pylori efficacy according to claim 1, characterized in that: In the step 2), during fermentation, the temperature is kept at 30-32° C. and the fermentation lasts for 18-20 days.

9. A composition having anti-Helicobacter pylori efficacy, characterized in that: The composition is prepared by the method for preparing the composition with anti-Helicobacter pylori efficacy according to any one of claims 1 to 8.

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