Type-III recombinant collagen-containing composition for effectively and painlessly improving skin and preparation method of type-III recombinant collagen-containing composition

Abstract

The invention provides a composition containing type III recombinant collagen and a preparation method of the composition. The composition is capable of effectively and painlessly improving the skin. The composition contains the type III recombinant collagen, retinol, modified hyaluronic acid and the like. According to the modified hyaluronic acid, firstly, hyaluronic acid, alginic acid and polyethylene glycol react with epichlorohydrin, a chloropropane group is grafted, then the modified hyaluronic acid reacts with chitosan for crosslinking, and a huge grid structure is formed. The unique grid structure contains a large number of amino and hydroxyl active groups, the moisturizing capacity and stability of hyaluronic acid can be remarkably improved, and the hyaluronic acid can be tightly combined with III type recombinant collagen, retinol and other components through hydrogen bonds, electrostatic interaction and other intermolecular acting forces to form a stable composite system. The composite system can reduce the release speed of each active component, reduce the irritation to the skin, and enhance the transdermal absorption efficiency, thereby significantly improving the overall efficacy of the formula.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and in particular to a composition containing type III recombinant collagen for effectively and painlessly improving skin quality and a preparation method thereof. Background Art

[0002] With the rapid development of the skin care industry, consumers' requirements for skin care products have gradually shifted from basic moisturizing and cleaning to functional needs, such as anti-aging, repairing the skin barrier, and improving skin quality. Collagen, as the main structural protein in the dermis of the skin, plays an important role in maintaining skin elasticity, promoting repair and anti-aging. Among them, type III collagen has attracted much attention due to its key role in skin fineness and wound repair. However, existing collagen skin care products still have many technical defects in practical applications, which limits their effects and user experience.

[0003] First, high irritation is one of the main problems in the existing technology. In order to achieve quick results, many skin care products add high concentrations of active ingredients (such as retinol), but these ingredients are often highly irritating to the skin, easily causing problems such as redness, desquamation, and dryness, especially for sensitive skin groups. In addition, the animal-derived collagen used in traditional collagen skin care products may have immunogenicity risks, further increasing the possibility of skin allergies or discomfort.

[0004] Secondly, the poor compatibility of the components leads to unsatisfactory effects in improving skin quality. In the prior art, the compatibility of collagen with other active ingredients (such as retinol, hyaluronic acid, etc.) is poor, and it is difficult to achieve synergistic effects. For example, hyaluronic acid, as a common moisturizing ingredient, can provide an immediate moisturizing effect, but its molecular structure is single and easily degraded by environmental influences, making it difficult to form a stable synergistic effect with collagen. In addition, the lack of compatibility of the ingredients in the formula may lead to a decrease in the stability of the active ingredients, affecting their long-term release and transdermal absorption, thereby reducing the overall effect of improving skin quality.

[0005] In summary, the existing technology has obvious deficiencies in terms of irritation, ingredient compatibility and skin quality improvement effect, and it is difficult to meet consumers' demand for efficient, gentle and safe skin care. It is urgent to develop a new technical solution to solve the problems existing in the existing technology and meet the development needs of the current market. Summary of the invention

[0006] Based on this, the present invention provides a composition containing type III recombinant collagen for effective and painless improvement of skin quality and a preparation method thereof, wherein the composition containing type III recombinant collagen for effective and painless improvement of skin quality contains components such as type III recombinant collagen, retinol and modified hyaluronic acid, while ensuring efficient improvement of skin quality, significantly reducing the irritation of the formula. Wherein, the modified hyaluronic acid first reacts hyaluronic acid, alginic acid and polyethylene glycol with epichlorohydrin, grafts chloropropane groups, and then reacts and crosslinks with chitosan to form a grid structure with a large structure. This unique grid structure contains a large number of amino and hydroxyl active groups, which can not only significantly improve the moisture retention ability and stability of hyaluronic acid, but also can be closely combined with components such as type III recombinant collagen and retinol through intermolecular forces such as hydrogen bonds and electrostatic effects to form a stable composite system. This composite system can reduce the release rate of each active component, reduce its irritation to the skin, enhance its transdermal absorption efficiency, ensure that the function of each component to improve skin quality is fully utilized, thereby significantly improving the overall efficacy of the formula.

[0007] One object of the present invention is to provide a composition containing type III recombinant collagen for effectively and painlessly improving skin quality, wherein the composition containing type III recombinant collagen for effectively and painlessly improving skin quality comprises the following components in parts by weight:

[0008]

[0009] in,

[0010] The modified hyaluronic acid is obtained by mixing hyaluronic acid, alginic acid and polyethylene glycol, then adding epichlorohydrin to react, and then reacting with chitosan to cross-link.

[0011] Furthermore, the skin conditioning agent peptide is selected from one or more of jojoba seed oil, allantoin, dipotassium glycyrrhizinate, ceramide, vitamin E, bisabolol or arginine.

[0012] Furthermore, the emulsifier is selected from one or more of alkyl glycoside, Tween 60 or Tween 80.

[0013] Furthermore, the moisturizing agent is selected from one or more of butylene glycol, dipropylene glycol, sorbitol, inositol, β-glucan, propylene glycol or glycerol.

[0014] Furthermore, the preservative is one or more of phenoxyethanol, parabens, p-hydroxyacetophenone or imidazolidinyl urea.

[0015] Another object of the present invention is to provide a method for preparing the composition containing type III recombinant collagen for effectively and painlessly improving skin quality, comprising the following steps:

[0016] S1, blending hyaluronic acid, alginic acid and polyethylene glycol, adding epichlorohydrin, adding a catalyst, heating for reaction, and obtaining an intermediate product;

[0017] S2, mixing the intermediate product, chitosan and sodium hydroxide, and heating them for reaction to obtain modified hyaluronic acid;

[0018] S3, adding water into the reaction kettle, adding the modified hyaluronic acid, type III recombinant collagen, retinol and skin conditioning agent under heating conditions, stirring and dissolving;

[0019] S4. Cool down, continue to add the remaining components, and then add them to an emulsifying homogenizer for uniform emulsification to obtain a composition containing type III recombinant collagen that can effectively and painlessly improve skin quality.

[0020] Furthermore, in step S1, the mass ratio of hyaluronic acid, alginic acid, polyethylene glycol and epichlorohydrin is 1:(1-3):(1-3):(10-30).

[0021] Furthermore, in step S1, the heating temperature is 50-80°C.

[0022] Furthermore, in step S2, the mass ratio of the intermediate product, chitosan and sodium hydroxide is 1:(10-30):(4-6).

[0023] Furthermore, in step S2, the heating temperature is 40-60°C.

[0024] Furthermore, in step S3, the heating temperature is 60-70°C.

[0025] The present invention has the following beneficial effects:

[0026] The composition containing type III recombinant collagen that effectively and painlessly improves skin quality provided by the present invention contains components such as type III recombinant collagen, retinol and modified hyaluronic acid, and while ensuring efficient improvement of skin quality, the irritation of the formula is significantly reduced. Wherein, the modified hyaluronic acid first reacts hyaluronic acid, alginic acid and polyethylene glycol with epichlorohydrin, grafts chloropropane groups, and then reacts and crosslinks with chitosan to form a grid structure with a huge structure. This unique grid structure contains a large number of amino and hydroxyl active groups, which can not only significantly improve the moisture retention ability and stability of hyaluronic acid, but also can be closely combined with components such as type III recombinant collagen and retinol through intermolecular forces such as hydrogen bonds and electrostatic effects to form a stable composite system. This composite system can reduce the release rate of each active component, reduce its irritation to the skin, enhance its transdermal absorption efficiency, ensure that the function of each component to improve skin quality is fully exerted, thereby significantly improving the overall efficacy of the formula. DETAILED DESCRIPTION

[0027] In order to more clearly illustrate the technical solution of the present invention, the following examples are listed. Unless otherwise stated, the raw materials, reactions and post-treatment methods shown in the examples are common raw materials on the market and technical methods well known to those skilled in the art.

[0028] The words "preferred", "preferably", "more preferably", etc. in the present invention refer to embodiments of the present invention that can provide certain beneficial effects in certain circumstances. However, other embodiments may also be preferred under the same circumstances or other circumstances. In addition, the description of one or more preferred embodiments does not imply that other embodiments are not applicable, nor is it intended to exclude other embodiments from the scope of the present invention.

[0029] It should be understood that, except in any operating examples, or where otherwise indicated, all numbers indicating, for example, the amounts of ingredients used in the specification and claims should be understood to be modified in all cases by the term "about". Therefore, unless indicated to the contrary, the numerical parameters set forth in the following specification and the appended claims are approximate values ​​that vary depending on the desired properties to be obtained by the present invention.

[0030] The following materials are used in the embodiments of the present invention:

[0031] Type III recombinant collagen: Recombinant type III human collagen was purchased from Jiangsu Chuangjian Medical Technology Co., Ltd.

[0032] Retinol: purchased from Wuhan Kamik Technology Co., Ltd.

[0033] Hyaluronic acid: purchased from Qingdao Future Biotechnology Co., Ltd.

[0034] Alginate: purchased from Shandong Pingju Biotechnology Co., Ltd.

[0035] Polyethylene glycol: molecular weight 20000, purchased from Hubei Baoling Chemical Technology Co., Ltd.

[0036] Boron trifluoride·ethyl ether complex: analytical grade, purchased from Tianjin Guangfu Fine Chemical Research Institute.

[0037] Ethylene glycol monoethyl ether: CP grade, purchased from Shanghai Reagent Factory No. 3.

[0038] Catalyst: Boron trifluoride·ethyl ether complex and ethylene glycol monoethyl ether are mixed in a volume ratio of 1:1.

[0039] Chitosan: purchased from Guangdong Mingcheng Biotechnology Co., Ltd.

[0040] Jojoba seed oil: skin conditioner, purchased from Guangzhou Huayang Biotechnology Co., Ltd.

[0041] Vitamin E: skin conditioning agent, vitamin E acetate 98%, purchased from Zhejiang Xinhecheng Co., Ltd.

[0042] Tween 80: emulsifier, purchased from Shandong Guohua Chemical Co., Ltd.

[0043] Propylene glycol: humectant.

[0044] Rose essential oil: essence, purchased from Guangzhou Hecheng Sanshen Biotechnology Co., Ltd.

[0045] Phenoxyethanol: preservative.

[0046] The water in the embodiments of the present invention is deionized water.

[0047] The "parts" in the embodiments of the present invention refer to parts by mass.

[0048] Example 1

[0049] A composition containing type III recombinant collagen for effectively and painlessly improving skin quality, the composition containing type III recombinant collagen for effectively and painlessly improving skin quality comprises the following components in parts by weight:

[0050]

[0051]

[0052] in,

[0053] The modified hyaluronic acid is obtained by mixing hyaluronic acid, alginic acid and polyethylene glycol, then adding epichlorohydrin to react, and then reacting with chitosan to cross-link.

[0054] The method for preparing the composition containing type III recombinant collagen for effectively and painlessly improving skin quality comprises the following steps:

[0055] S1. Using water as solvent, hyaluronic acid, alginic acid and polyethylene glycol are mixed, stirred and dissolved at 50°C, and then epichlorohydrin is added (hyaluronic acid:alginic acid:polyethylene glycol:epichlorohydrin=1:1:2:25, m / m / m / m), a catalyst (1% by mass of reactants) is added, and the mixture is reacted at 70°C for 10 hours, centrifuged and dried to obtain an intermediate product;

[0056] S2. Using water as solvent, the intermediate product, chitosan and sodium hydroxide are mixed (intermediate product: chitosan: sodium hydroxide = 1:20:5, m / m / m / m), reacted at 40° C. for 12 h, filtered and dried to obtain modified hyaluronic acid;

[0057] S3, adding water according to the above mass fractions into the reaction kettle, adding the modified hyaluronic acid, type III recombinant collagen, retinol and skin conditioning agent at 70°C, stirring to dissolve;

[0058] S4. Cool down to 50°C, continue to add the remaining components, and then add them into an emulsifying homogenizer for uniform emulsification. After vacuum degassing, filtering and cooling, a composition containing type III recombinant collagen that can effectively and painlessly improve skin quality is obtained.

[0059] Example 2

[0060] A composition containing type III recombinant collagen for effectively and painlessly improving skin quality, the composition containing type III recombinant collagen for effectively and painlessly improving skin quality comprises the following components in parts by weight:

[0061]

[0062] in,

[0063] The modified hyaluronic acid is obtained by mixing hyaluronic acid, alginic acid and polyethylene glycol, then adding epichlorohydrin to react, and then reacting with chitosan to cross-link.

[0064] The preparation method of the above-mentioned composition containing type III recombinant collagen for effectively and painlessly improving skin quality is the same as that in Example 1.

[0065] Example 3

[0066] A composition containing type III recombinant collagen for effectively and painlessly improving skin quality, the composition containing type III recombinant collagen for effectively and painlessly improving skin quality comprises the following components in parts by weight:

[0067]

[0068] in,

[0069] The modified hyaluronic acid is obtained by mixing hyaluronic acid, alginic acid and polyethylene glycol, then adding epichlorohydrin to react, and then reacting with chitosan to cross-link.

[0070] The preparation method of the above-mentioned composition containing type III recombinant collagen for effectively and painlessly improving skin quality is the same as that in Example 1.

[0071] Comparative Example 1

[0072] A composition containing type III recombinant collagen for effectively and painlessly improving skin quality. The difference between this comparative example and Example 1 is that step S2 is omitted, and in step S3, the modified hyaluronic acid and other qualities are replaced by the intermediate product. Other ingredients and preparation methods are the same as those in Example 1.

[0073] Comparative Example 2

[0074] A composition containing type III recombinant collagen for effectively and painlessly improving skin quality. The difference between this comparative example and Example 1 is that step S1 is omitted, and in step S2, the mass of the intermediate product is replaced by hyaluronic acid, and other ingredients and preparation methods are the same as those in Example 1.

[0075] Test Case

[0076] The anti-aging effect comparison test was performed on Examples 1-3 and Comparative Examples 1-2.

[0077] Test method: 30 volunteers (women aged 23 to 40, with the average R2 value of the left and right cheekbones of the left and right faces not higher than 0.65) were recruited to participate in the anti-aging effect test. The volunteers used the composition prepared by Examples 1-3 and Comparative Examples 1-2 once a day in the morning and evening for 4 consecutive weeks. Before using the composition, and after 1 week, 2 weeks and 4 weeks of use, the skin moisture (Corneometer CM825) and skin elasticity (Cutometer MPA580) of one random cheek were tested.

[0078] The test results are shown in Table 1.

[0079] Table 1 Anti-aging effect test results

[0080]

[0081] It can be seen from the above experimental data that the compositions of Examples 1-3 of the present invention are non-irritating to the skin. Compared with before use, the moisture content of the skin has been significantly improved after 4 weeks of use of the compositions of Examples 1-3 of the present invention, increasing by 27.7%, 28.3% and 26.5% respectively (P < 0.05), indicating that the present invention has a good moisturizing effect. Compared with before use, the skin elasticity R2 parameter has been significantly improved after 4 weeks of use of the compositions of Examples 1-3 of the present invention, increasing by 12.2%, 13.0% and 12.3% respectively (P < 0.05), indicating that the present invention has a good effect of improving skin elasticity. However, the modification of hyaluronic acid in Comparative Examples 1 and 2 is incomplete, and it has a certain irritation to the skin, and the effects of moisturizing and improving skin quality are not as good as those of the present invention.

[0082] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.

[0083] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A composition containing type III recombinant collagen for effectively and painlessly improving skin quality, characterized in that: The composition containing type III recombinant collagen for effectively and painlessly improving skin quality comprises the following components in parts by weight: in, The modified hyaluronic acid is obtained by mixing hyaluronic acid, alginic acid and polyethylene glycol, then adding epichlorohydrin to react, and then reacting with chitosan to cross-link.

2. The composition containing type III recombinant collagen for effectively and painlessly improving skin quality according to claim 1, characterized in that: The skin conditioning agent peptide is selected from one or more of jojoba seed oil, allantoin, dipotassium glycyrrhizinate, ceramide, vitamin E, bisabolol or arginine.

3. The composition containing type III recombinant collagen for effectively and painlessly improving skin quality according to claim 1, characterized in that: The emulsifier is selected from one or more of alkyl glycoside, Tween 60 or Tween 80.

4. The composition containing type III recombinant collagen for effectively and painlessly improving skin quality according to claim 1, characterized in that: The moisturizing agent is selected from one or more of butylene glycol, dipropylene glycol, sorbitol, inositol, β-glucan, propylene glycol or glycerol.

5. A method for preparing a composition containing type III recombinant collagen for effectively and painlessly improving skin quality according to any one of claims 1 to 4, characterized in that: The steps include: S1, blending hyaluronic acid, alginic acid and polyethylene glycol, adding epichlorohydrin, adding a catalyst, heating for reaction, and obtaining an intermediate product; S2, mixing the intermediate product, chitosan and sodium hydroxide, and heating them for reaction to obtain modified hyaluronic acid; S3, adding water into the reaction kettle, adding the modified hyaluronic acid, type III recombinant collagen, retinol and skin conditioning agent under heating conditions, stirring and dissolving; S4. Cool down, continue to add the remaining components, and then add them to an emulsifying homogenizer for uniform emulsification to obtain a composition containing type III recombinant collagen that can effectively and painlessly improve skin quality.

6. The method for preparing the composition containing type III recombinant collagen for effectively and painlessly improving skin quality according to claim 5, characterized in that: In step S1, the mass ratio of hyaluronic acid, alginic acid, polyethylene glycol and epichlorohydrin is 1:(1-3):(1-3):(10-30).

7. The method for preparing the composition containing type III recombinant collagen for effectively and painlessly improving skin quality according to claim 5, characterized in that: In step S1, the heating temperature is 40-60°C.

8. The method for preparing the composition containing type III recombinant collagen for effectively and painlessly improving skin quality according to claim 5, characterized in that: In step S2, the mass ratio of the intermediate product, chitosan and sodium hydroxide is 1:(10-30):(4-6).

9. The method for preparing the composition containing type III recombinant collagen for effectively and painlessly improving skin quality according to claim 5, characterized in that: In step S2, the heating temperature is 40-60°C.

10. The method for preparing the composition containing type III recombinant collagen for effectively and painlessly improving skin quality according to claim 5, characterized in that: In step S3, the heating temperature is 60-70°C.

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