Dyeing device for studying three-level lymph structure in tumor microenvironment

Abstract

The invention relates to the technical field of medical instruments, and provides a dyeing device for studying a three-level lymphatic structure in a tumor microenvironment, the dyeing device comprises a base, one side of the inner bottom wall of the base is fixedly connected with a mounting seat, the front side of the top wall of the mounting seat is provided with a placing groove, and the middle of the top wall of the mounting seat is fixedly connected with a stirring barrel; a collecting groove is formed in the rear side of the top wall of the mounting base, one end of the liquid inlet pipe is arranged in the containing groove, one end of the liquid inlet pipe penetrates through the side wall of the machine base, a cavity is formed in the lower portion in the mounting base, a water pump is fixedly connected to the inner wall of the cavity, one end of the water pump communicates with the stirring barrel, and the other end of the water pump communicates with the collecting groove. Under the combined action of the machine base, the mounting base, the placing groove, the stirring barrel, the liquid inlet pipe, the water inlet pipe, the quantitative barrel, the liquid discharging pipe, the collecting groove, the cavity and the pump body, the dyeing agent can be automatically diluted and dyed, the dyeing convenience of workers is improved, and the dyeing efficiency is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and in particular to a staining device for studying tertiary lymphatic structures in a tumor microenvironment. Background Art

[0002] Breast cancer is one of the major malignant tumors in the world. It accounts for a large proportion of cancer-related deaths in women due to its large heterogeneity, high degree of malignancy and poor prognosis. In recent years, studies have shown that the expression of immune checkpoints in tumor-infiltrating immune cells and the adaptive immune system is of great significance in the prognosis of breast cancer. The interaction between tumor cells and the tumor microenvironment is a complex and evolving process, which requires the identification of more effective biomarkers to improve the precision immunotherapy of breast cancer patients. The tertiary lymphoid structure has many similarities with lymph nodes in structure and development. It generally appears in inflammatory tissues caused by chronic inflammation, autoimmune diseases and tumors, and plays a key role in determining prognosis and immunotherapy response. Among them, the patient group with a higher score of the tertiary lymphoid structure showed a significantly improved overall survival rate compared with the patient group with a lower score. Therefore, the study of the tertiary lymphoid structure in the tumor microenvironment is particularly important.

[0003] In the process of studying the tertiary lymphatic structure, a staining device is needed to stain the slices. The slice staining device, also called a tissue slice stainer or a tissue slice stainer, is a laboratory equipment used for staining biological tissue slices. It is widely used in pathology, cytology, medical research, and clinical diagnosis. These devices are usually used to stain specimens that have been histologically sectioned in order to observe the morphology and structural characteristics of tissue cells through a microscope.

[0004] In the process of staining the tertiary lymphatic structure, the DAPI staining solution needs to be diluted 1:100 in sterile distilled water before staining and incubating the slices. However, the current automatic staining devices often lack dilution and mixing structures for different liquids. Usually, the staff directly pours the finished stain into the staining box in the staining device for staining. For example, in the case of staining that requires dilution during the research process, the staff needs to manually dilute and mix the stain before staining. This results in a decrease in staining efficiency and an increase in the risk of error in manual mixing. Therefore, the current commonly used stains have limited functions and the actual use effect has certain limitations. Summary of the invention

[0005] In view of the deficiencies of the prior art, the present invention provides a staining device for studying tertiary lymphatic structures in a tumor microenvironment, which solves the problem that the stain can be automatically diluted and proportioned.

[0006] To achieve the above objectives, the present invention is implemented through the following technical solutions: a staining device for studying tertiary lymphatic structures in a tumor microenvironment, comprising a machine base, a mounting seat is fixedly connected to one side of the inner bottom wall of the machine base, a placement groove is provided on the front side of the top wall of the mounting seat, a stirring bucket is fixedly connected to the middle of the top wall of the mounting seat, a collecting groove is provided on the rear side of the top wall of the mounting seat, a quantitative cylinder is fixedly connected to the inner side wall of the machine base, a liquid inlet pipe is connected to the front side of the lower part of the quantitative cylinder, a water inlet pipe is connected to the rear side of the lower part of the quantitative cylinder, a liquid discharge pipe is connected to the bottom wall of the quantitative cylinder, one end of the liquid inlet pipe is arranged in the placement groove, one end of the water inlet pipe runs through the side wall of the machine base, one end of the liquid discharge pipe is connected to the stirring bucket, a chamber is provided in the lower part of the mounting base, a water pump is fixedly connected to the inner wall of the chamber, one end of the water pump is connected to the stirring bucket, and the other end of the water pump is connected to the collecting tank;

[0007] A quantitative component, which is arranged in the quantitative cylinder and can discharge the liquid quantitatively;

[0008] A stirring component is arranged in the stirring barrel, and the stirring component can fully stir and mix different liquids.

[0009] Preferably, the quantitative component includes a bracket, which is fixedly connected to the rear side of the outer wall of the quantitative cylinder, and the bottom wall of the bracket is fixedly connected to a servo motor, the output end of the servo motor passes through the inner bottom wall of the bracket and is fixedly connected to a threaded rod, the outer periphery of the threaded rod is threadedly connected to a moving block, both sides of the top wall of the moving block are fixedly connected to connecting rods, the top ends of the two connecting rods pass through the top wall of the bracket and are fixedly connected to connecting blocks, the front side of the bottom wall of the connecting block is fixedly connected to a fixing rod, the bottom end of the fixing rod passes through the quantitative cylinder, the front and rear sides of the quantitative cylinder are provided with grooves, the lower parts of the two grooves are slidably connected to floating blocks, and the close ends of the floating blocks and the fixed rods are fixedly connected to sensors.

[0010] Preferably, the stirring assembly includes a driving motor, which is fixedly connected to the top wall of the stirring barrel, and the output end of the driving motor passes through the inner top wall of the stirring barrel and is fixedly connected to gear 1, and the outer periphery of the inner top wall of the stirring barrel is fixedly connected to a gear ring, and a plurality of gears 2 are meshed between gear 1 and the gear ring, and the bottom walls of the plurality of gears 2 are fixedly connected to spiral rods, and the bottom wall of gear 1 is fixedly connected to a stirring rod.

[0011] Preferably, a clamping device is provided on the inner rear wall of the base, and evenly distributed site boxes are provided on the inner bottom wall of the base.

[0012] Preferably, a control panel is fixedly connected to one side of the front wall of the base.

[0013] Preferably, limit rods are provided on both sides of the threaded rod, the bottom walls of the two limit rods are fixedly connected to the inner bottom wall of the bracket, and the top ends of the two brackets pass through the moving block and are fixedly connected to the inner top wall of the bracket.

[0014] Preferably, springs are fixedly connected to the front and rear sides of the top wall of the floating block, and the top ends of the two springs are fixedly connected to the top wall inside the groove.

[0015] Preferably, the inner bottom walls of the two grooves are fixedly connected with guide rods, the top ends of the two guide rods pass through the floating blocks and are fixedly connected to the inner top walls of the grooves, and the two springs are arranged on the outer periphery of the guide rods.

[0016] Preferably, a visible window is fixedly connected to one side of the outer circumference of the quantitative cylinder, and the visible window penetrates into the quantitative cylinder.

[0017] Preferably, an annular groove is provided on the inner top wall of the mixing barrel, and a plurality of the second top walls of the gears are fixedly connected with limit blocks, and a plurality of the limit blocks are engaged in the annular groove.

[0018] Working principle: In actual use, the staff can put the dye box to be diluted and mixed into the placement slot 4, and by starting the servo motor 15, the threaded rod 16 can be driven to rotate and drive the moving block 17 to move up and down. When the moving block 17 moves, it can drive the connecting rod 12, the connecting block 11 and the fixed rod 13 to move up and down. When it moves to the appropriate position, the dye is sent into the quantitative cylinder 8 through the liquid inlet pipe 6, so that the floating block 22 can be pushed up. When the two sensors 21 are in contact, the liquid inlet pipe 6 is closed and the discharge pipe 9 is opened, so that a certain amount of dye is input into the mixing barrel 5. As the dye is fully discharged, the floating block 22 will move down and separate the sensor 21 and after a period of time, the discharge pipe 9 is closed. At this time, the servo motor 15 is started again to adjust the position of the fixed rod 13 according to the dilution ratio, and then the water inlet pipe 7 can be opened to the quantitative cylinder. 8 is input with sterile distilled water for dilution and the float 22 is pushed up again. When the sensor 21 contacts again, the water inlet pipe 7 is closed and the discharge pipe 9 is opened, so that the sterile distilled water in the quantitative cylinder 8 can be sent into the stirring barrel 5. After the distilled water and the chromosomes are introduced into the stirring barrel 5, the drive motor 28 is started to drive the gear 1 29 to rotate, and under the joint action of the gear ring 31, the gear 2 30 can be driven to rotate and revolve around the axis of the gear 1 29 at the same time. When the gear 1 29 rotates, it can drive the stirring rod 33 to rotate. When the gear 2 30 rotates, it can drive the spiral rod 34 to rotate and revolve, so that the dye and the sterile distilled water are fully diluted and mixed. When the dye is diluted, the water pump 27 can be started to extract it from the stirring barrel 5 and send it into the collecting tank 10, so that the slice can be stained with the dye.

[0019] The present invention provides a staining device for studying the tertiary lymphatic structure in the tumor microenvironment. It has the following beneficial effects:

[0020] 1. The present invention can automatically dilute and dye the dye through the joint action of the machine base, the mounting base, the placement tank, the stirring barrel, the liquid inlet pipe, the water inlet pipe, the quantitative cylinder, the liquid discharge pipe, the collecting tank, the chamber and the pump body, thereby improving the dyeing convenience of the staff and increasing the dyeing efficiency.

[0021] 2. The present invention can quantitatively transport the dye to be diluted and the sterile distilled water used for dilution through the joint action of the servo motor, threaded rod, moving block, connecting rod, connecting block, fixed rod, floating block, groove and sensor, thereby ensuring the accuracy of the dilution ratio.

[0022] 3. The present invention drives the stirring rod and the spiral rod to dilute and mix the dye and sterile distilled water through the joint action of the driving motor, gear 1, gear ring, gear 2, stirring rod and spiral rod, and the spiral rod can revolve while rotating, so that the bottom layer of liquid can be transported to the upper layer while stirring, thereby improving the stirring and mixing efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic diagram of the front structure of the present invention;

[0024] Figure 2 It is a schematic diagram of the connection structure of the mounting base in the present invention;

[0025] Figure 3 It is a schematic cross-sectional view of the stirring assembly structure in the present invention;

[0026] Figure 4 Schematic diagram of the connection structure of the gear ring in the present invention;

[0027] Figure 5 It is a schematic diagram of the structure of the quantitative component in the present invention;

[0028] Figure 6 It is a schematic cross-sectional view of the quantitative component structure in the present invention;

[0029] Figure 7 It is a schematic cross-sectional view of the structure of the mounting seat in the present invention.

[0030] Among them, 1. base; 2. control panel; 3. mounting base; 4. placement slot; 5. stirring barrel; 6. liquid inlet pipe; 7. water inlet pipe; 8. metering cylinder; 9. liquid discharge pipe; 10. collecting tank; 11. connecting block; 12. connecting rod; 13. fixing rod; 14. bracket; 15. servo motor; 16. threaded rod; 17. moving block; 18. limiting rod; 19. visual window; 20. ring groove; 21. sensor; 22. floating block; 23. groove; 24. guide rod; 25. spring; 26. chamber; 27. water pump; 28. driving motor; 29. ​​gear one; 30. gear two; 31. gear ring; 32. limiting block; 33. stirring rod; 34. spiral rod; 35. clamping device; 36. station box. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the specification of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] Please see attached Figure 1 and attached Figure 7 The embodiment of the present invention provides a staining device for studying the tertiary lymphatic structure in the tumor microenvironment, including a machine base 1, a mounting seat 3 is fixedly connected to one side of the inner bottom wall of the machine base 1, a placement groove 4 is provided on the front side of the top wall of the mounting seat 3, a dye box to be diluted is placed in the placement groove 4, a stirring bucket 5 is fixedly connected to the middle of the top wall of the mounting seat 3, a collecting groove 10 is provided on the rear side of the top wall of the mounting seat 3, and the collecting groove 10 can be used to hold the mixed and diluted dye box, a quantitative cylinder 8 is fixedly connected to the inner wall of the machine base 1, a liquid inlet pipe 6 is connected to the front side of the lower part of the quantitative cylinder 8, and a water inlet pipe 7 is connected to the rear side of the lower part of the quantitative cylinder 8, and a micro pump is respectively used to feed water between the water inlet pipe 7 and the liquid inlet pipe 6 and the quantitative cylinder 8. The bottom wall of the metering cylinder 8 is connected with a drain pipe 9, and the drain pipe 9 and the metering cylinder 8 are connected through a solenoid valve. One end of the liquid inlet pipe 6 is arranged in the placement groove 4, and one end of the water inlet pipe 7 passes through the side wall of the machine base 1. One end of the drain pipe 9 is connected with the stirring barrel 5. A chamber 26 is opened in the lower part of the mounting base 3, and a water pump 27 is fixedly connected to the inner wall of the chamber 26. One end of the water pump 27 is connected with the stirring barrel 5, and the other end of the water pump 27 is connected with the collecting tank 10. One end of the water pump 27 is connected with the bottom wall of the stirring barrel 5, and the other end of the water pump 27 is connected with the upper part of the collecting tank 10, and the collecting tank 10 and the dye box matching the collecting tank 10 are provided with holes for communicating with the water pump 27.

[0033] The staff can open the dye box to be diluted and place it in the placement tank 4, then the staff can put the liquid inlet pipe 6 into the dye box in the placement tank 4, and adjust the quantitative value of the quantitative cylinder 8 to the liquid, and start the micro pump body connected to the liquid inlet pipe 6 to extract the dye in the placement tank 4 and send it to the quantitative cylinder 8. When the amount of dye entering the quantitative cylinder 8 reaches a preset value set in advance, the micro pump body connected to the liquid inlet pipe 6 and the quantitative cylinder 8 is closed and the liquid inlet pipe 6 is sealed, and then the electromagnetic valve connected between the discharge pipe 9 and the quantitative cylinder 8 is opened, so that the dye in the quantitative cylinder 8 is discharged and sent into the stirring barrel 5 through the discharge pipe 9, and then the electromagnetic valve is closed and the staff adjusts the quantitative cylinder 8 again. The quantitative value is determined and the micro pump connected to the water inlet pipe 7 and the quantitative cylinder 8 is started, so that the sterile distilled water can be pumped out through the water inlet pipe 7 and sent into the quantitative cylinder 8. When the sterile distilled water entering the quantitative cylinder 8 reaches the preset value, the micro pump connected to the water inlet pipe 7 and the quantitative cylinder 8 is closed and the water inlet pipe 7 is sealed. At the same time, the solenoid valve is opened again to allow the sterile distilled water in the quantitative cylinder 8 to flow into the stirring barrel 5, so that the sterile distilled water and the dye are mixed and diluted. When the dye is diluted, it can be pumped out from the stirring barrel 5 and sent into the dyeing box in the collecting tank 10 through the water pump 27, so that the slices are dyed to achieve the effect of dynamically diluting the dye, improving the dyeing convenience of the staff and increasing the dyeing efficiency.

[0034] Please see attached Figure 1 , Attachment Figure 5 and attached Figure 6 , a quantitative component, which is arranged in the quantitative cylinder 8 and can discharge the liquid quantitatively;

[0035] The quantitative component includes a bracket 14, which is fixedly connected to the rear side of the outer wall of the quantitative cylinder 8. A servo motor 15 is fixedly connected to the bottom wall of the bracket 14. The output end of the servo motor 15 passes through the inner bottom wall of the bracket 14 and is fixedly connected to a threaded rod 16. The outer periphery of the threaded rod 16 is threadedly connected to a moving block 17. Connecting rods 12 are fixedly connected to both sides of the top wall of the moving block 17. The top ends of the two connecting rods 12 pass through the top wall of the bracket 14 and are fixedly connected to a connecting block 11. A fixed rod 13 is fixedly connected to the front side of the bottom wall of the connecting block 11. The bottom end of the fixed rod 13 passes through the quantitative cylinder 8. The bottom end size of the fixed rod 13 is larger than the size of the remaining parts of the fixed rod 13. Grooves 23 are provided on the front and rear sides of the quantitative cylinder 8. Floating blocks 22 are slidably connected to the lower parts of the two grooves 23. The floating blocks 22 are located at the lowest part of the grooves 23 in the initial state and are higher than the position where the water inlet pipe 7 and the liquid inlet pipe 6 are connected to the quantitative cylinder 8. Sensors 21 are fixedly connected to the close ends of the floating blocks 22 and the fixed rods 13, and the two sensors 21 are located on the same axis.

[0036] By starting the servo motor 15, the threaded rod 16 connected to the output end can be driven to rotate. When the threaded rod 16 rotates, the movable block 17 connected to the periphery can be driven to move. When the movable block 17 moves, the connected connecting rod 12 can be driven to move. When the connecting rod 12 moves, the fixed connecting block 11 can be driven to move. Through the connecting block 11, the fixed fixing rod 13 and the sensor 21 fixed on the bottom wall of the fixing rod 13 can be driven to move up and down.

[0037] Therefore, when the staff needs to quantitatively deliver dye or sterile distilled water, the staff can start the servo motor 15 and drive the fixed rod 13 and the sensor 21 connected to the fixed rod 13 to move to the height corresponding to the quantitative value. Then, after the dye enters the quantitative cylinder 8, it will push the float 22 to move upward along the groove 23, and the float 22 will also drive the sensor 21 connected to it to move while moving. When the fixed rod 13 and the sensor 21 of the float 22 contact each other, that is, the amount of dye or sterile distilled water in the quantitative cylinder 8 reaches the preset value, at this time, the dye is moved to the quantitative cylinder. The pipeline for conveying liquid in 8 is closed, and the pipeline for discharging liquid in the quantitative cylinder 8 is opened, so that the dye or sterile distilled water in the quantitative cylinder 8 is discharged, and as the water level of the liquid in the quantitative cylinder 8 drops, the float 22 will also drive the corresponding sensor 21 to drop with the water level. When the two sensors 21 are separated, the pipeline for discharge will be closed after a certain period of time according to the preset value set in advance by the staff, so as to achieve quantitative delivery of the dye to be diluted and the sterile distilled water used for dilution, and ensure the accuracy of the dilution ratio.

[0038] Please see attached Figure 1 , Attachment Figure 3 and attached Figure 4 , a stirring component, which is arranged in the stirring barrel 5, and the stirring component can fully stir and mix different liquids.

[0039] The stirring assembly includes a driving motor 28, which is fixedly connected to the top wall of the stirring barrel 5. The output end of the driving motor 28 passes through the inner top wall of the stirring barrel 5 and is fixedly connected to a gear 1 29. A gear ring 31 is fixedly connected to the outer periphery of the inner top wall of the stirring barrel 5. A plurality of gear 2s 30 are meshed between the gear 1 29 and the gear ring 31. The bottom walls of the plurality of gear 2s 30 are fixedly connected to a spiral rod 34. The bottom wall of the gear 1 29 is fixedly connected to a stirring rod 33.

[0040] By starting the drive motor 28, the gear 1 29 fixed at the output end can be driven to rotate. When the gear 1 29 rotates, the meshing gear 2 30 can be driven to rotate. Since the gear 2 30 is meshed with the gear 1 29 and the gear ring 31 at the same time, and the gear ring 31 is fixed to the inner wall of the mixing barrel 5, the gear 2 30 can be driven to rotate while revolving around the axis of the gear 1 29 under the joint action of the gear ring 31 and the gear 1 29. When the gear 1 29 rotates, the stirring rod 33 can be driven to rotate. When the gear 2 30 rotates, the screw rod 34 can be driven to rotate and revolve.

[0041] Therefore, after the dye and sterile distilled water enter the mixing barrel 5, the staff can start the drive motor 28 to drive the stirring rod 33 and the screw rod 34 to rotate. When the screw rod 34 revolves, it can cooperate with the stirring rod 33 to expand the stirring range and improve the stirring efficiency under the rotation speed difference. At the same time, the screw rod 34 can transport the liquid in the lower layer to the upper layer or the liquid in the upper layer to the lower layer when rotating, thereby avoiding stratification of the mixed liquid and improving the effect of mixing and dilution, so that the dye and sterile distilled water are fully diluted and mixed.

[0042] Please see attached Figure 1 A clamping device 35 is provided on the inner rear wall of the base 1 , and evenly distributed site boxes 36 are provided on the inner bottom wall of the base 1 .

[0043] By providing a clamping device 35, the slice rack can be clamped and moved to facilitate different staining processes for the slices. At the same time, the staff can divide the multiple station boxes 36 into reagent stations, water washing stations and reagent heating stations. The clamping device 35 provided in conjunction can clamp and move the slice rack, so that the paraffin on the surface of the slice can be cleaned and dewaxed before the slice is stained. The dewaxed slice can continue to be stained. At the same time, the multiple station boxes 36 are detachably connected to the machine base 1. Therefore, after the slice staining is completed, the staff can remove the station box 36 divided into reagent stations and recycle the staining reagents therein, thereby saving costs and recycling multiple times.

[0044] Please see attached Figure 1 A control panel 2 is fixedly connected to one side of the front wall of the base 1.

[0045] The provision of the control panel 2 makes it convenient for the staff to operate the control panel 2 to control the dyeing device.

[0046] Please see attached Figure 5 and attached Figure 6Limit rods 18 are provided on both sides of the threaded rod 16 , and the bottom walls of the two limit rods 18 are fixedly connected to the inner bottom wall of the bracket 14 , and the top ends of the two brackets 14 pass through the moving block 17 and are fixedly connected to the inner top wall of the bracket 14 .

[0047] The limit rod 18 is provided to limit the moving block 17 , thereby preventing the moving block 17 from rotating along with the threaded rod 16 . Therefore, when the threaded rod 16 rotates, the moving block 17 can be driven to move along the threaded rod 16 .

[0048] Please see attached Figure 6 Springs 25 are fixedly connected to the front and rear sides of the top wall of the floating block 22 , and the top ends of the two springs 25 are fixedly connected to the top wall inside the groove 23 .

[0049] The spring 25 can be compressed when the float 22 moves upward. Therefore, when the liquid in the metering cylinder 8 is discharged, the spring 25 can provide an opposite force and push the float 22 to reset, so as to ensure the stability of the reset of the float 22.

[0050] Please see attached Figure 6 The inner bottom walls of the two grooves 23 are fixedly connected with guide rods 24 , the top ends of the two guide rods 24 pass through the floating blocks 22 and are fixedly connected to the inner top walls of the grooves 23 , and the two springs 25 are arranged on the outer periphery of the guide rods 24 .

[0051] The guide rod 24 can limit the spring 25 , thereby preventing the spring 25 from bending when being compressed, thereby ensuring the working stability of the spring 25 .

[0052] Please see attached Figure 5 A visual window 19 is fixedly connected to one side of the outer circumference of the metering cylinder 8 , and the visual window 19 penetrates into the metering cylinder 8 .

[0053] The visual window 19 is provided to facilitate the staff to observe the situation in the metering cylinder 8 through the visual window 19 .

[0054] Please see attached Figure 3 An annular groove 20 is formed in the top wall of the mixing barrel 5 , and the top walls of the plurality of gears 30 are fixedly connected to limit blocks 32 , and the plurality of limit blocks 32 are engaged in the annular groove 20 .

[0055] By providing the cooperation between the annular groove 20 and the limiting block 32 , the limiting block 32 can be limitedly engaged, so that the gear 2 30 connected to the limiting block 32 can be limited, thereby ensuring the stability of the gear 2 30 in rotation.

[0056] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A staining device for studying tertiary lymphatic structures in a tumor microenvironment, comprising a base (1), characterized in that: A mounting seat (3) is fixedly connected to one side of the inner bottom wall of the machine base (1); a placement groove (4) is provided on the front side of the top wall of the mounting seat (3); a stirring bucket (5) is fixedly connected to the middle of the top wall of the mounting seat (3); a collecting groove (10) is provided on the rear side of the top wall of the mounting seat (3); a dosing cylinder (8) is fixedly connected to the inner side wall of the machine base (1); a liquid inlet pipe (6) is connected to the front side of the lower part of the dosing cylinder (8); a water inlet pipe (7) is connected to the rear side of the lower part of the dosing cylinder (8); and the dosing cylinder (8) is connected to the inner side wall of the machine base (1). The bottom wall of the measuring cylinder (8) is connected to a liquid discharge pipe (9), one end of the liquid inlet pipe (6) is arranged in the placement groove (4), one end of the water inlet pipe (7) passes through the side wall of the machine base (1), one end of the liquid discharge pipe (9) is connected to the stirring barrel (5), a chamber (26) is opened in the lower part of the mounting base (3), a water pump (27) is fixedly connected to the inner wall of the chamber (26), one end of the water pump (27) is connected to the stirring barrel (5), and the other end of the water pump (27) is connected to the collecting tank (10); A quantitative component, the quantitative component is arranged in the quantitative cylinder (8), and the quantitative component is capable of quantitatively discharging liquid; A stirring component, wherein the stirring component is arranged in a stirring barrel (5), and the stirring component can fully stir and mix different liquids.

2. A staining device for studying tertiary lymphatic structures in tumor microenvironment according to claim 1, characterized in that: The quantitative assembly comprises a bracket (14), the bracket (14) being fixedly connected to the rear side of the outer wall of the quantitative cylinder (8), the bottom wall of the bracket (14) being fixedly connected to a servo motor (15), the output end of the servo motor (15) passing through the inner bottom wall of the bracket (14) and being fixedly connected to a threaded rod (16), the outer periphery of the threaded rod (16) being threadedly connected to a moving block (17), both sides of the top wall of the moving block (17) being fixedly connected to connecting rods (12), the top ends of the two connecting rods (12) passing through the top wall of the bracket (14) and being fixedly connected to a connecting block (11), the front side of the bottom wall of the connecting block (11) being fixedly connected to a fixing rod (13), the bottom end of the fixing rod (13) passing through the inside of the quantitative cylinder (8), the front and rear sides of the inside of the quantitative cylinder (8) being provided with grooves (23), the lower parts of the two grooves (23) being slidably connected to floating blocks (22), the ends of the floating blocks (22) close to the fixed rods (13) being fixedly connected to sensors (21).

3. A staining device for studying tertiary lymphatic structures in tumor microenvironment according to claim 1, characterized in that: The stirring assembly comprises a driving motor (28), the driving motor (28) being fixedly connected to the top wall of the stirring barrel (5), the output end of the driving motor (28) penetrating the inner top wall of the stirring barrel (5) and being fixedly connected to a gear 1 (29), a gear ring (31) being fixedly connected to the outer periphery of the inner top wall of the stirring barrel (5), a plurality of gear 2s (30) being meshed between the gear 1 (29) and the gear ring (31), the bottom walls of the plurality of gear 2s (30) being fixedly connected to a spiral rod (34), and the bottom wall of the gear 1 (29) being fixedly connected to a stirring rod (33).

4. A staining device for studying tertiary lymphatic structures in tumor microenvironment according to claim 1, characterized in that: The inner rear wall of the machine base (1) is provided with a clamping device (35), and the inner bottom wall of the machine base (1) is provided with evenly distributed station boxes (36).

5. The staining device for studying tertiary lymphatic structures in tumor microenvironment according to claim 1, characterized in that: A control panel (2) is fixedly connected to one side of the front wall of the machine base (1).

6. A staining device for studying tertiary lymphatic structures in tumor microenvironment according to claim 2, characterized in that: Limiting rods (18) are provided on both sides of the threaded rod (16); the bottom walls of the two limiting rods (18) are fixedly connected to the inner bottom wall of the bracket (14); and the top ends of the two brackets (14) pass through the moving block (17) and are fixedly connected to the inner top wall of the bracket (14).

7. A staining device for studying tertiary lymphatic structures in tumor microenvironment according to claim 2, characterized in that: Springs (25) are fixedly connected to the front and rear sides of the top wall of the floating block (22), and the top ends of the two springs (25) are fixedly connected to the inner top wall of the groove (23).

8. A staining device for studying tertiary lymphatic structures in tumor microenvironment according to claim 7, characterized in that: The inner bottom walls of the two grooves (23) are fixedly connected with guide rods (24), the top ends of the two guide rods (24) penetrate the floating blocks (22) and are fixedly connected to the inner top walls of the grooves (23), and the two springs (25) are arranged on the outer periphery of the guide rods (24).

9. A staining device for studying tertiary lymphatic structures in tumor microenvironment according to claim 2, characterized in that: A visible window (19) is fixedly connected to one side of the outer circumference of the metering cylinder (8), and the visible window (19) penetrates into the metering cylinder (8).

10. The staining device for studying tertiary lymphatic structures in tumor microenvironment according to claim 3, characterized in that: The inner top wall of the mixing barrel (5) is provided with an annular groove (20), the top walls of the plurality of gears 2 (30) are all fixedly connected to limit blocks (32), and the plurality of limit blocks (32) are all engaged in the annular groove (20).

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