Zinc and baicalein compounded nano material, preparation method and application

Abstract

The embodiment of the invention relates to a zinc and baicalein composite nano material, a preparation method and application, the preparation method comprises the following steps: mixing a baicalein solution and a zinc salt solution, and then carrying out a self-assembly reaction under room temperature and dark conditions to obtain the metal zinc and baicalein composite nano material, the zinc and baicalein compounded nano material disclosed by the invention has photothermal conversion performance, relatively good bactericidal ability and long-term antibacterial ability.

Description

Technical Field

[0001] The present application relates to the technical field of antibacterial materials, and more specifically, to a zinc and baicalein composite nanomaterial, a preparation method and an application thereof. Background Art

[0002] Baicalin is a phenolic antioxidant with a wide range of biological activities, including antibacterial, antiviral, antioxidant and anti-inflammatory effects. Baicalin has a wide range of antibacterial effects and has inhibitory effects on most Gram-negative and Gram-positive bacteria. Its antibacterial mechanism may be related to inhibiting bacterial metabolism and destroying bacterial cell walls and cell membranes. However, the antibacterial effect of baicalin alone is limited. Summary of the invention

[0003] The present application is provided to solve the above defects in the prior art. A zinc and baicalein composite nanomaterial, preparation method and application are needed to enhance the antibacterial ability of baicalein and have excellent photothermal conversion efficiency.

[0004] According to an embodiment of the present application, a nanomaterial composite of zinc and baicalein is provided, wherein the nanomaterial comprises one or more structures shown in Formula I, Formula II and Formula III: (I); (Ⅱ); (III). In some embodiments, the nanomaterial has a diameter of 70-110 nm.

[0005] According to an embodiment of the present application, a method for preparing the nanomaterial described in any embodiment of the present application is also provided, the preparation method comprising: mixing a baicalein solution and a zinc salt solution, causing a self-assembly reaction at room temperature and in the dark to obtain a nanomaterial composite of metallic zinc and baicalein.

[0006] In some embodiments, the concentration of the baicalein solution is 2 mg / mL-8 mg / mL; the concentration of the zinc salt solution is 0.5-1.5 mg / mL.

[0007] In some embodiments, the self-assembly reaction time is 3 h-10 h.

[0008] In some embodiments, the pH of the self-assembly reaction is 8-9.

[0009] According to an embodiment of the present application, there is also provided an application of the nanomaterial described in any embodiment of the present application or the method for preparing the nanomaterial described in any embodiment of the present application in a photothermal conversion material.

[0010] According to the embodiments of the present application, there is also provided an application of the nanomaterial described in any embodiment of the present application or the method for preparing the nanomaterial described in any embodiment of the present application in an antibacterial material.

[0011] In some embodiments, the concentration of the composite nanomaterial of metallic zinc and baicalein in the solution is 0.1-5 mg / mL.

[0012] In some embodiments, the nanomaterial is irradiated with laser.

[0013] The zinc and baicalein composite nanomaterials, preparation methods and applications provided in various embodiments of the present application, the metal zinc in the zinc and baicalein composite nanomaterials has the function of carrying baicalein into bacteria, providing a metal channel for entering bacteria, improving the bactericidal ability of the nanomaterial, and utilizing the advantages of the mutual cooperation of metal zinc and baicalein to have long-term antibacterial ability; at the same time, it has photothermal conversion performance, so it can fully utilize thermal energy during sterilization and enhance the bactericidal effect; in addition, it can also give full play to the advantages of the nanomaterial itself, can better contact with bacteria, and has strong applicability and broad application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In the drawings, which are not necessarily drawn to scale, the same reference numerals may describe similar parts in different views. The same reference numerals with letter suffixes or different letter suffixes may represent different instances of similar parts. The accompanying drawings generally illustrate various embodiments by way of example and not limitation, and together with the specification and claims, are used to illustrate the embodiments applied for. When appropriate, the same reference numerals are used throughout the drawings to refer to the same or similar parts. Such embodiments are illustrative and are not intended to be exhaustive or exclusive embodiments of the present apparatus or method.

[0015] Figure 1 The infrared spectrum of the zinc-scutellaria nanomaterial according to Example 1 of the present application is shown; Figure 2 The first X-ray electron spectrum of the zinc-scutellaria nanomaterial according to Example 1 of the present application is shown; Figure 3 A second X-ray electron spectrum diagram of the zinc-scutellaria nanomaterial according to Example 1 of the present application is shown; Figure 4 A transmission electron microscope image of the zinc-scutellaria nanomaterial according to Example 1 of the present application is shown; Figure 5 The diameter distribution diagram of the zinc-scutellaria nanomaterial according to Example 1 of the present application is shown; Figure 6 The potential detection diagram of the zinc-scutellaria nanomaterial according to Example 1 of the present application is shown; Figure 7 A nitrogen adsorption detection diagram of the zinc-scutellaria nanomaterial according to Example 1 of the present application is shown; Figure 8 Shows the ultraviolet absorption graph of zinc-scutellaria nanomaterials according to different embodiments of the present application; Fig. 9 Shows the laser absorption diagram of zinc-scutellaria nanomaterial solutions of different concentrations according to the present application; Fig.10 A comparison diagram showing the antibacterial effect of nanomaterials of different concentrations on Escherichia coli according to an embodiment of the present application; Fig.11 A diagram showing the light-to-heat conversion efficiency of ultraviolet absorption according to an embodiment of the present application; Fig.12 A comparison diagram showing the antibacterial effect of nanomaterials of different concentrations on Escherichia coli according to an embodiment of the present application; Fig.13 A graph showing the results of a long-term antibacterial experiment on Escherichia coli using the nanomaterial according to an embodiment of the present application; Fig.14 A diagram showing the circulation effect of the nanomaterial according to an embodiment of the present application on Escherichia coli; Fig.15 A graph showing the results of a long-term antibacterial experiment on Staphylococcus aureus by the nanomaterial according to an embodiment of the present application; Fig.16 A diagram showing the circulation effect of the nanomaterial according to an embodiment of the present application on Staphylococcus aureus. DETAILED DESCRIPTION

[0016] In order to enable those skilled in the art to better understand the technical solution of the present application, the present application is described in detail below in conjunction with the accompanying drawings and specific implementation examples. The embodiments of the present application are further described in detail below in conjunction with the accompanying drawings and specific implementation examples, but are not intended to limit the present application.

[0017] The words "first", "second" and similar words used in this application do not indicate any order, quantity or importance, but are only used to distinguish. The words "include" or "comprise" and similar words mean that the elements before the word include the elements listed after the word, and do not exclude the possibility of including other elements.

[0018] According to an embodiment of the present application, a nanomaterial composite of zinc and baicalein is provided, wherein the nanomaterial comprises one or more structures shown in Formula I, Formula II and Formula III: (I); (Ⅱ); (III). Baicalein has three hydroxyl groups, and zinc is connected to the hydroxyl groups of baicalein. Metallic zinc can enter bacteria, and the compound of metallic zinc and baicalein can enter bacteria under the action of metallic zinc, so that baicalein can play a bactericidal and antibacterial effect inside bacteria. Therefore, the zinc-baicalein nanomaterial of the present application has a better bactericidal effect than baicalein alone.

[0019] In addition, zinc-baicalin nanomaterials have good photothermal conversion properties and can absorb light energy and convert it into thermal energy. Zinc-baicalin nanomaterials can use the converted heat to enhance their antibacterial ability.

[0020] In some embodiments, the diameter of the nanomaterial is 70-110 nm. The zinc-baicalein of the nanomaterial can better exert its antibacterial ability.

[0021] According to an embodiment of the present application, a method for preparing the nanomaterial described in any embodiment of the present application is also provided, the preparation method comprising: mixing a baicalein solution and a zinc salt solution, and then performing a self-assembly reaction at room temperature and in the dark to obtain a nanomaterial composite of metallic zinc and baicalein. The preparation method of the present application has low energy consumption, is relatively easy to operate, and is conducive to wide application.

[0022] In some embodiments, the concentration of the baicalein solution is 2 mg / mL-8 mg / mL; the concentration of the zinc salt solution is 0.5-1.5 mg / mL. Preferably, the concentration of the baicalein solution is 3 mg / mL-6 mg / mL; the concentration of the zinc salt solution is 0.8-1.2 mg / mL. The solvent of the baicalein solution may be ethanol or the like. Ultrasound or the like may be used to assist in the rapid dissolution of baicalein. The solvent of the zinc salt solution may be water, and the water may be purified water, ultrapure water, or the like. In addition, baicalein and zinc salt may be directly mixed in water containing ethanol, dissolved directly, and then reacted.

[0023] In some embodiments, the volume ratio of the baicalein solution to the zinc salt solution is 0.5-2:1.

[0024] In some embodiments, the molar ratio of baicalein to zinc salt in the nanomaterial is 1-5:1, preferably 2-4:1.

[0025] In some embodiments, the zinc salt includes zinc nitrate or zinc chloride, etc.

[0026] In some embodiments, the self-assembly reaction time is 3h-10h. Preferably, the self-assembly reaction time is 5-7h, such as 5h, 6h, 7h, etc. The self-assembly reaction process does not require external additional conditions, and the reaction conditions are relatively easy to achieve, which is conducive to a wide range of applications.

[0027] In some embodiments, the pH of the self-assembly reaction is 8-9. Preferably, the pH is 8. The pH can be adjusted using sodium hydroxide or the like. By adjusting the pH value to a suitable value during the self-assembly reaction, the self-assembly reaction is facilitated to obtain a zinc-baicalein composite nanomaterial.

[0028] According to the embodiments of the present application, there is also provided an application of the nanomaterial described in any embodiment of the present application or the preparation method of the nanomaterial described in any embodiment of the present application in a photothermal conversion material. The zinc-baicalein nanomaterial of the present application can absorb light energy and convert it into heat energy, and has a high conversion efficiency. Therefore, it can be used in photothermal conversion materials.

[0029] According to the embodiments of the present application, there is also provided an application of the nanomaterial described in any embodiment of the present application or the preparation method of the nanomaterial described in any embodiment of the present application in an antibacterial material. Metal zinc can be used as an inorganic antibacterial agent, and the antibacterial effect of metal ions is utilized to achieve the purpose of long-term antibacterial effect. It has better antibacterial effect at high temperature, will not produce drug resistance, has longer antibacterial effect, and the effect will not be reduced. Through the combined effect of metal zinc and baicalein, it has better bactericidal effect and long-term antibacterial ability than both metal zinc and baicalein alone, and can be used repeatedly.

[0030] In some embodiments, the concentration of the zinc and baicalein composite nanomaterial in the solution is 0.1-5 mg / mL, preferably 0.1-2 mg / mL, and further preferably 0.3-2 mg / mL. The zinc and baicalein composite nanomaterial can be directly dissolved in water and used at room temperature, without complex and harsh use conditions, and is widely used.

[0031] In some embodiments, the nanomaterial is irradiated with a laser having a wavelength in the range of 200-900 nm.

[0032] Example 1 Step 1, add 121 mg of baicalin to 30 mL of ethanol solution, stir and mix evenly, and ultrasonicate for 30 min to fully dissolve it; then add 47 mg of zinc nitrate to 50 mL of ultrapure water. Step 2, take 10 mL of baicalin solution and 6 mL of zinc nitrate solution, mix and add them to a reaction bottle, keep warm and avoid light at room temperature, stir and react for 6 hours, and adjust the pH of the mixed solution to 8 by NaOH; Step 3, after the reaction is completed, remove the supernatant, wash the precipitate with ethanol and water for 2 to 3 times, centrifuge at 6000 rpm, and dry to obtain zinc-scutellaria nanomaterial A, which includes one or more structures shown in formula I, formula II and III.

[0033] The zinc-scutellaria nanomaterial A obtained in Example 1 and baicalein alone were subjected to infrared spectroscopy detection. Figure 1 The infrared spectrum shown in FIG. 1 shows a curve A1 representing the infrared spectrum curve of baicalein alone, and curve A2 representing the infrared spectrum curve of zinc-scutellaria nanomaterial A. The horizontal axis represents the wavelength, and the vertical axis represents the transmittance. Figure 1 It can be obtained that the zinc-scutellaria nanomaterial A contains baicalin.

[0034] The zinc-scutellaria nanomaterial A obtained in Example 1 was subjected to X-ray electron spectroscopy (XPS) detection. Figure 2 and Figure 3 The XPS test results shown in the figure have the binding energy on the horizontal axis and the intensity on the vertical axis. Figure 2 It can be obtained that the zinc-scutellaria nanomaterial A contains CO bonds, C=O bonds and Zn-O bonds; Figure 3 It can be obtained that the zinc-scutellaria nanomaterial A contains a Zn-O bond, which is a coordination bond, and Z2P1 / 2 and Z2P3 / 2 indicate that zinc is divalent. This indicates that zinc and baicalin are connected by chemical bonds, rather than just adsorbed composites, which is conducive to zinc playing the role of a metal channel for entering bacteria. Baicalin retains carbon-oxygen double bonds and carbon-oxygen single bonds in other rings, while zinc forms a chemical bond with oxygen, indicating that zinc is connected to the hydroxyl group of baicalin. According to the valence of zinc, the zinc-scutellaria nanomaterial A can be obtained as a structure shown in formula I.

[0035] The zinc-scutellaria nanomaterial A obtained in Example 1 was examined by transmission electron microscopy (TEM), and the following Figure 4 The results shown; and the particle size statistics of zinc-scutellaria nanomaterial A were obtained as follows Figure 5 The results are shown. Figure 4 and Figure 5 It can be obtained that the diameter of the zinc-scutellaria nanomaterial A is 70-110nm; in addition, through the lens diagram, it can be obtained that the nanomaterial has good dispersion, which is conducive to more complete contact with bacteria.

[0036] The zinc-scutellaria nanomaterial A obtained in Example 1 was tested for potential, and the following was obtained: Figure 6 The results are shown. Figure 6 The potential can be obtained as -7V.

[0037] The zinc-scutellaria nanomaterial A obtained in Example 1 was subjected to nitrogen adsorption detection, and the following Figure 7 The results shown are shown in Figure 1. The horizontal axis is relative pressure and the vertical axis is relative pressure. Figure 7 It can be obtained that the zinc-scutellaria nanomaterial A has a porous structure, the pore radius ranges from 6 to 20 nm, and the single-point average pore radius is about 10.04 nm. In this way, the zinc-scutellaria nanomaterial A can fully exert the adsorption effect and perform antibacterial.

[0038] Example 2 Step 1, add 121 mg of baicalin to 30 mL of ethanol solution, stir and mix evenly, and ultrasonicate for 30 min to fully dissolve it; then add 47 mg of zinc nitrate to 50 mL of ultrapure water. Step 2, take 10 mL of baicalin solution and 10 mL of zinc nitrate solution and add them to a reaction bottle, keep warm and avoid light at room temperature, stir and react for 7 hours, and adjust the pH of the mixed solution to 8 by NaOH; Step 3, after the reaction is completed, remove the supernatant, wash the precipitate with ethanol and water for 2 to 3 times, centrifuge at 6000 rpm, and dry to obtain zinc-scutellaria nanomaterial B, and the obtained zinc-scutellaria nanomaterial A includes one or more structures shown in formula I, formula II and III.

[0039] Example 3 Step 1, add 121 mg of baicalin to 30 mL of ethanol solution, stir and mix evenly, and ultrasonicate for 30 min to fully dissolve it; then add 47 mg of zinc nitrate to 50 mL of ultrapure water. Step 2 Take 10 mL of baicalin solution and 12 mL of zinc nitrate solution and add them to a reaction bottle, keep warm and avoid light at room temperature, stir and react for 4 hours, and adjust the pH of the mixed solution to 8 by NaOH; Step 3, after the reaction is completed, remove the supernatant, wash the precipitate with ethanol and water for 2 to 3 times, centrifuge at 7000 rpm, and dry to obtain zinc-scutellaria nanomaterial C, and the obtained zinc-scutellaria nanomaterial A includes one or more structures shown in formula I, formula II and III.

[0040] Experimental Example 1 The zinc-baicalein composite nanomaterials prepared in Example 1, Example 2 and Example 3 were used to prepare a nanomaterial solution with a concentration of 0.025 mg / mL. The nanomaterial solution was a solution of the zinc-baicalein composite nanomaterial and water. A photothermal performance experiment was conducted, and baicalein alone was added as a comparative example. The photothermal performance experiment conditions included: irradiating the nanomaterial solution with light for 10 minutes. Figure 8 The results shown, Figure 8 The horizontal axis is wavelength, and the vertical axis is absorbance. Figure 8 In the direction of the arrows, from bottom to top, the B4-B1 curves correspond to the baicalein solution, Example 2, Example 3, and Example 1. Figure 8 It was found that the absorbance of pure baicalein to ultraviolet light between 200-500nm was between 0-0.4, the absorbance of the nanomaterial obtained in Example 2 to ultraviolet light was between 0-0.8, the absorbance of the nanomaterial obtained in Example 3 to ultraviolet light was between 0-0.8, and the absorbance of the nanomaterial obtained in Example 1 to ultraviolet light was between 0-1.2. Compared with pure baicalein, the nanomaterial has a higher absorbance and better light absorption performance.

[0041] Experimental Example 2 The zinc-baicalein composite nanomaterial prepared by the nanomaterial of Example 1 was used to prepare nanomaterial solutions with concentrations of 2 mg / mL, 3 mg / mL and 4 mg / mL, respectively. The nanomaterial solution was a solution of the zinc-baicalein composite nanomaterial and water. A photothermal performance experiment was conducted, and baicalein alone and metallic zinc alone were added as comparative examples. The photothermal performance experiment conditions included: irradiating the nanomaterial solution with 2W laser power for 10 minutes. Fig. 9 The results shown, Fig. 9 The horizontal axis is wavelength, and the vertical axis is absorbance. Fig. 9 In the direction of the arrows, from bottom to top, the D5-D1 curves correspond to baicalein solution, metallic zinc, 2 mg / mL, 3 mg / mL, and 4 mg / mL. Fig. 9 It can be obtained that the absorbance of metallic zinc alone is basically 0, and baicalein alone has only weak light absorption performance. However, as the concentration of the nanomaterial solution increases, the light absorption performance increases, and the absorbance of a 4 mg / mL nanomaterial solution is about 4.3.

[0042] Experimental Example 3 Antimicrobial activity test: 1. Grow E. coli to OD 600 = 0.5 (optical density of bacterial solution), add different concentrations of nanomaterials of Example 1 (0, 0.1, 0.5, 1, 1.5 and 2, unit is mg / mL), and perform a group of photothermal experiments, then incubate at 37°C for 30 minutes, take 100μl of supernatant, add 20μl of MTT (3-methyl-tetrahydrothiazolidine-2-thione), place at 37°C in the dark for 1h, and add 100μl of DMSO (dimethyl sulfoxide). The photothermal experiment group is irradiated with laser at 808nm at the same time, and the following is obtained: Fig.10 The results shown, Fig.10 The horizontal axis is the concentration, and the vertical axis is the survival rate of E. coli. Fig.10 In the two vertical bars corresponding to each concentration horizontal axis, the left side is the group without photothermal treatment, and the right side is the group with photothermal treatment. Then, a photothermal cycle experiment was performed on 4 mg / mL of nanomaterials using a laser at 808 nm to obtain a complete rising and cooling curve. The data was calculated according to the photothermal conversion efficiency formula as follows: Fig.11 The results shown, Fig.11 The horizontal axis is -lnθ. Fig.11 The data can be used to obtain a linear fitting relationship between time t and -lnθ, and the light-to-heat conversion efficiency can be obtained. It can be obtained that the light-to-heat conversion efficiency reaches 15.8%, indicating that the nanomaterial of the present application has good light-to-heat conversion performance.

[0043] pass Fig.10 It can be seen that the experimental group using laser irradiation has a better antibacterial effect than the one using nanomaterials alone, and the sterilization rate of Escherichia coli is higher, indicating that the photothermal conversion of nanomaterials is beneficial to improving the antibacterial effect. And the antibacterial effect of nanomaterial solutions increases with the increase of concentration in the range of 0-2mg / mL.

[0044] 2. Cultivate Staphylococcus aureus (S. A) to OD 600 =0.5, add different concentrations of materials (0, 0.1, 0.3, 0.5, 0.8 and 1, the unit is mg / mL), and perform a group of light and heat experiments, then incubate at 37℃ for 30 minutes, take 100μl of the supernatant, add 20μl of MTT, place at 37℃ in the dark for 1h, add 100μl of DMSO, and obtain the following Fig.12 The results shown, Fig.12 In the two vertical bars corresponding to each concentration horizontal axis, the left side is the experiment group without photothermal treatment, and the right side is the experiment group with photothermal treatment. The photothermal experiment group was irradiated with 808nm laser. Fig.12 The horizontal axis is the concentration, and the vertical axis is the survival rate of Staphylococcus aureus.

[0045] pass Fig.12 It can be seen that the experimental group using laser irradiation has a better antibacterial effect than the one using nanomaterials alone, and the sterilization rate of Staphylococcus aureus is higher, indicating that the photothermal conversion of nanomaterials is beneficial to improving the antibacterial effect. And the antibacterial effect of the nanomaterial solution increases with the increase of concentration in the range of 0-1 mg / mL.

[0046] Experimental Example 4 Long-term and cyclic antibacterial activity experiments were conducted on the nanomaterials of Example 1: In the cyclic antibacterial experiment, Escherichia coli (OD 600 = 0.4) and zinc-baicalein nanomaterials (3 mg / mL) were incubated at room temperature for 2 days, and the OD 600 Then, E. coli was removed by centrifugation at 1000 rpm to recover the zinc-baicalein nanomaterials and the E. coli (OD 600 = 0.4) suspension and incubated at room temperature for 2 days. This experiment was repeated five times in total. After each test, a certain amount of the suspension was diluted and inoculated on LB (Luria broth) agar. After incubation at 37°C overnight, the plates were photographed. In the long-term antibacterial experiment, the zinc-baicalein nanomaterial (3 mg / mL) suspension was also used to incubate E. coli (OD 600 = 0.4), a small amount of suspension was taken at 2, 4, 6, 8, 10, 12, and 14 days of incubation to measure OD 600, and then diluted in LB medium. After incubation at 37℃ overnight, take pictures. 600 = 0.4) as the control, incubate at room temperature for 2 days, and measure OD 600 The suspension was diluted, spread on plates, cultured at 37°C overnight, and photographed.

[0047] In the cyclic antibacterial experiment, Staphylococcus aureus (OD 600 = 0.4) and zinc-baicalein nanomaterials (3 mg / mL) were incubated at room temperature for 2 days, and the OD 600 Then, the S. aureus was removed by centrifugation at 1000 rpm to recover the Zn-baicalin nanomaterials. 600 = 0.4) suspension and incubated at room temperature for 2 days. This experiment was repeated five times in total. After each test, a certain amount of the suspension was diluted and inoculated on LB agar. After incubation at 37°C overnight, the plates were photographed. In the long-term antibacterial experiment, the zinc-baicalein nanomaterial (3 mg / mL) suspension was also used to incubate Staphylococcus aureus (OD 600 = 0.4), a small amount of suspension was taken at 2, 4, 6, 8, 10, 12, and 14 days of incubation to measure OD 600 , and then diluted in LB medium. After incubation at 37℃ overnight, take pictures. 600 = 0.4) as the control, incubate at room temperature for 2 days, and measure OD 600 The suspension was diluted, spread on plates, cultured at 37°C overnight, and photographed.

[0048] For E. coli, the results of long-term antibacterial experiments are as follows Fig.13 As shown in the figure, within 14 days, the survival rate of E. coli on the second day was about 21%; on the 4th to 6th day, the survival rate of E. coli was 10%-20%; from the 6th to the 14th day, the survival rate of E. coli was less than 10%; at the 14th day, it still had a high antibacterial rate, and the survival rate of E. coli was still able to remain less than 10%. The results of the cycle experiment are shown in the figure. Fig.14 As shown, when the nanomaterials were recycled 5 times, the survival rate of E. coli was less than 32%.

[0049] For Staphylococcus aureus, the results of long-term antibacterial experiments are as follows Fig.15 As shown in the figure, within 14 days, the survival rate of E. coli on the second day was less than 10%; on the 4th to 6th day, the survival rate of E. coli was 10%-20%; from the 8th to the 14th day, the survival rate of E. coli was less than 10%; at the 14th day, it still had a high antibacterial rate, and the survival rate of E. coli was still able to remain less than 10%. The results of the cycle experiment are shown in the figure. Fig.16During the five-time recycling of the nanomaterial shown, the survival rate of Escherichia coli was less than 20% within two cycles; during the third cycle, the survival rate of Escherichia coli was about 42%; during the fourth cycle, the survival rate of Escherichia coli was less than 20%; during the fifth cycle, the survival rate of Escherichia coli was about 21%.

[0050] Therefore, the nanomaterials can have strong antibacterial properties against Escherichia coli and Staphylococcus aureus, and the antibacterial properties are persistent, with a period of 14 days, and still maintain high antibacterial properties after 14 days, so the antibacterial ability can even reach more than 14 days, which has good application prospects.

[0051] Nanomaterials can be reused, and a single use will not significantly reduce their antibacterial ability. They can repeatedly exert their antibacterial effects and reduce the consumption of nanomaterials.

[0052] In addition, although exemplary embodiments have been described herein, the scope includes any and all embodiments based on the present application with equivalent elements, modifications, omissions, combinations (e.g., various embodiments intersecting schemes), adaptations or changes. The elements in the claims are to be interpreted broadly based on the language adopted in the claims, and are not limited to the examples described in this specification or during the practice of this application, and the examples are to be interpreted as non-exclusive. Therefore, this specification and examples are intended to be considered as examples only, and the true scope and spirit are indicated by the following claims and the full scope of their equivalents.

[0053] The above description is intended to be illustrative rather than restrictive. For example, the above examples (or one or more schemes thereof) may be used in combination with each other. For example, a person of ordinary skill in the art may use other embodiments when reading the above description. In addition, in the above-mentioned specific embodiments, various features may be grouped together to simplify the present application. This should not be interpreted as an intention that a feature of an application that does not require protection is necessary for any claim. On the contrary, the subject matter of the present application may be less than all the features of an embodiment of a particular application. Thus, the following claims are incorporated herein in the specific embodiments as examples or embodiments, wherein each claim is independently a separate embodiment, and it is considered that these embodiments may be combined with each other in various combinations or arrangements. The scope of the present invention should be determined with reference to the appended claims and the full scope of equivalent forms granted by these claims.

[0054] The above embodiments are only exemplary embodiments of the present application and are not intended to limit the present invention. The protection scope of the present invention is defined by the claims. Those skilled in the art may make various modifications or equivalent substitutions to the present invention within the essence and protection scope of the present application, and such modifications or equivalent substitutions shall also be deemed to fall within the protection scope of the present invention.

Claims

1. A zinc and baicalein composite nanomaterial, characterized in that: The nanomaterial includes one or more structures shown in Formula I, Formula II and Formula III: (I); (Ⅱ); (Ⅲ)。 2. The nanomaterial according to claim 1, characterized in that The diameter of the nano material is 70-110 nm.

3. A method for preparing the nanomaterial according to claim 1 or 2, characterized in that: The preparation method comprises: After the baicalein solution and the zinc salt solution are mixed, a self-assembly reaction occurs at room temperature and in the dark, thereby obtaining a nano material composited with metal zinc and baicalein.

4. The preparation method according to claim 3, characterized in that: The concentration of the baicalein solution is 2 mg / mL-5 mg / mL; the concentration of the zinc salt solution is 0.5-1.5 mg / mL.

5. The preparation method according to claim 3, characterized in that: The self-assembly reaction time is 3h-10h.

6. The preparation method according to claim 3, characterized in that: The pH of the self-assembly reaction is 8-9.

7. Use of the nanomaterial according to any one of claims 1 to 2 or the method for preparing the nanomaterial according to any one of claims 3 to 6 in photothermal conversion materials.

8. Use of the nanomaterial according to any one of claims 1 to 2 or the preparation method of the nanomaterial according to any one of claims 3 to 6 in antibacterial materials.

9. The use according to claim 8, characterized in that: The concentration of the metal zinc and baicalein composite nanomaterial in the solution is 0.1-5 mg / mL.

10. The use according to claim 8, characterized in that: The nanomaterial is irradiated with laser.

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