A microfluidic detection device for food additives

Abstract

This invention discloses a microfluidic detection device for food additives, comprising a first microfluidic chip including a first lower chip body, with a plurality of first liquid reservoirs at the upper end of the first lower chip body, the first liquid reservoirs being connected to one end of an outlet channel, the outlet channel being controllably connected to a first mixing channel or a second mixing channel; and a second microfluidic chip including a second upper chip body and a second lower chip body fixed to the lower side of the second upper chip body, with an antigen reservoir, a first antibody reservoir, a cleaning reservoir, a second antibody reservoir, and a luminescent substrate reservoir on the second lower chip body being controllably connected to one end of a third mixing channel, and a microchannel being opened at the upper end of the second lower chip body at the other end of the third mixing channel; this invention enables the detection of nitrite using two methods with a single microfluidic chip, improving the reliability of nitrite detection.

Description

Technical Field

[0001] This invention relates to the field of food additive detection technology, and in particular to a microfluidic detection device for food additives. Background Technology

[0002] Chloramphenicol is a broad-spectrum antibiotic that inhibits both Gram-positive and Gram-negative bacteria. In food, chloramphenicol is an illegal additive and must not be detected in animal-derived foods. Because chloramphenicol can inhibit the hematopoietic function of human bones, causing diseases such as aplastic anemia, its use in food production and processing is strictly prohibited. Amantadine was one of the earliest antiviral drugs used to inhibit influenza viruses, but it is now listed as a prohibited veterinary drug and must not be detected in animal-derived foods. Long-term consumption of animal-derived foods with excessive levels of amantadine may have adverse effects on human health. Sudan I is an industrial dye mainly used to color oils, waxes, and plastics, and is not a food additive. It has potential carcinogenic effects, and its addition to food would pose a serious threat to human health. Therefore, Sudan I is strictly prohibited from use in food production and processing. Current technologies for detecting these food additives mainly rely on large-scale equipment, which is not only time-consuming but also complex to operate, and generally can only detect single substances, making it difficult to meet the needs for rapid and efficient detection. Summary of the Invention

[0003] The purpose of this section is to outline some aspects of embodiments of the present invention and to briefly describe some preferred embodiments. Simplifications or omissions may be made in this section, as well as in the abstract and title of this application, to avoid obscuring the purpose of these documents; however, such simplifications or omissions should not be construed as limiting the scope of the invention.

[0004] In view of the problems existing in the above and / or existing food additive testing, the present invention is proposed.

[0005] Therefore, the purpose of this invention is to provide a microfluidic detection device for food additives, which enables the detection of nitrite by two methods through the setting of a first microfluidic chip, thereby improving the reliability of nitrite detection; and through the setting of a second microfluidic chip, the solutions in different storage tanks can be controlled to exit in a set reaction sequence, thereby indirectly measuring chloramphenicol, amantadine and Sudan I.

[0006] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a microfluidic detection device for food additives, comprising,

[0007] The first microfluidic chip includes a first upper chip body and a first lower chip body fixed on the lower side of the first upper chip body. The upper end of the first lower chip body is provided with a plurality of first liquid storage pools, a plurality of liquid outlet channels corresponding to the first liquid storage pools, a first mixing channel, a second mixing channel, a first waste liquid pool and a second waste liquid pool. One end of the liquid outlet channel is connected to the first liquid storage pool, and the other end of the liquid outlet channel is controllably connected to one end of the first mixing channel or one end of the second mixing channel. The other end of the second mixing channel is connected to the second waste liquid pool.

[0008] The second microfluidic chip includes a second upper chip body and a second lower chip body fixed below the second upper chip body. The upper end of the second lower chip body has an antigen reservoir, a first antibody reservoir, a cleaning reservoir, a second antibody reservoir, a luminescent substrate reservoir, and a third mixing channel. The antigen reservoir, the first antibody reservoir, the cleaning reservoir, the second antibody reservoir, and the luminescent substrate reservoir are controllably connected to one end of the third mixing channel. The other end of the second lower chip body at the third mixing channel has a microchannel. A silicone membrane coated with antigen is sandwiched between the second upper chip body and the second lower chip body at the microchannel. The upper end of the second lower chip body away from the third mixing channel has a third waste liquid pool.

[0009] As a preferred embodiment of the microfluidic detection device for food additives in this invention, the first microfluidic chip further includes a first movable valve, a second movable valve, and a third movable valve. A first movable channel is opened at the upward-facing end of the first lower chip body between several liquid outlet channels and the first mixing channel or the second mixing channel. The first movable valve can slide along the first movable channel. A second movable channel is opened on the first lower chip body between the first mixing channel and the first waste liquid pool. The second movable valve can slide along the second movable channel. A third movable channel is opened on the first lower chip body between the first mixing channel and the first waste liquid pool, and between the second mixing channel and the second waste liquid pool. The third movable valve can slide along the third movable channel. The first movable valve enables several liquid storage pools to controllably connect with the first mixing channel or the second mixing channel. The second movable valve enables the first mixing channel to connect with the first waste liquid pool or the other end of the first mixing channel to connect with the second mixing channel. The third movable valve enables the other end of the first mixing channel to connect with the first waste liquid pool or the end of the second mixing channel to connect with the second waste liquid pool.

[0010] In a preferred embodiment of the microfluidic detection device for food additives in this invention, the first moving valve includes a first moving transmission part outside the first lower chip body. A first moving part is fixed on the first moving transmission part and inserted into the first moving channel. A plurality of first liquid storage tanks are arranged with corresponding liquid outlets. There are three first liquid storage tanks. When one end of two of the liquid outlets is connected to two of the first liquid storage tanks, the other end of the two liquid outlets is connected to the first mixing channel. When one end of three liquid outlets is connected to three of the first liquid storage tanks, the other end of two of the liquid outlets is connected to the first mixing channel, and the other end of the other liquid outlet is connected to the beginning of the second mixing channel.

[0011] In a preferred embodiment of the microfluidic detection device for food additives in this invention, the second moving valve includes a second moving transmission part outside the first lower chip body. A second moving part is fixed on the second moving transmission part and inserted into the second moving channel. The second moving part has a first connecting mixing liquid hole and a second connecting mixing liquid hole. When one end of the first connecting mixing liquid hole is connected to the end of the first mixing channel, the other end of the first connecting mixing liquid hole is connected to the first waste liquid pool. The second connecting mixing liquid hole is offset from any mixing channel or waste liquid pool. When one end of the second connecting mixing liquid hole is connected to the end of the first mixing channel, the other end of the second connecting mixing liquid hole is connected to the second mixing channel.

[0012] In a preferred embodiment of the microfluidic detection device for food additives in this invention, the third moving valve includes a third moving transmission part outside the first lower chip body. A third moving part is fixed on the third moving transmission part and inserted into the third moving channel. The third moving part has a first connecting waste liquid hole and a second connecting waste liquid hole. When the two ends of the first connecting waste liquid hole are respectively connected to the first mixing channel and the first waste liquid pool, the two ends of the second connecting waste liquid hole are respectively offset from the second mixing channel and the second waste liquid pool. When the two ends of the second connecting waste liquid hole are respectively connected to the second mixing channel and the second waste liquid pool, the two ends of the first connecting waste liquid hole are respectively offset from the second mixing channel and the second waste liquid pool.

[0013] In a preferred embodiment of the microfluidic detection device for food additives in this invention, a fourth moving channel is provided on the second lower chip body between the storage tank and the third mixing channel. The second microfluidic chip also includes a fourth moving valve, which is a fourth moving transmission part outside the second lower chip body. The fourth moving transmission part has a fourth insertion hole. A fourth moving part that is just inserted into the fourth moving channel is fixed on the fourth moving transmission part. The fourth moving part has two first liquid passage holes and one second liquid passage hole. When one end of the two first liquid passage holes is connected to the antigen storage tank and the first antibody storage tank respectively, the other end of the first liquid passage hole is connected to one end of the third mixing channel. The second liquid passage hole is offset from any other storage tank. When one end of the second liquid passage hole is connected to the cleaning storage tank, the second antibody storage tank, or the luminescent substrate storage tank, the other end of the second liquid passage hole is connected to one end of the third mixing channel. The two first liquid passage holes are offset from any other storage tank.

[0014] As a preferred embodiment of the microfluidic detection device for food additives in this invention, it further includes: a detection housing, within which a fixed frame is fixedly connected. A horizontally movable seat is connected to the fixed frame. The movable seat is equipped with a first injection component for injecting solution into a first microfluidic chip and detecting the food additive to be detected, and a second injection component for injecting solution into each reservoir in a second microfluidic chip and detecting the food additive to be detected. The first injection component includes a first connecting bracket connected to the movable seat, with a plurality of first injection brackets connected to the first connecting bracket. A plurality of first lifting motors are fixedly connected to the first injection brackets. A first injection connecting block is slidably connected to the first injection bracket below the first lifting motor. A first injection liquid tube is connected to the first injection connecting block. The first injection screw, corresponding to the first lifting motor, is rotatably connected to the bracket. The first injection connecting block is threadedly connected to the corresponding first injection screw. The first injection tube can inject the solution into the corresponding first storage tank. The second injection assembly includes a second connecting bracket connected to the movable base. A plurality of second injection brackets are connected to the second connecting bracket. A plurality of second lifting motors are fixedly connected to the second injection bracket. A second injection connecting block is slidably connected to the second injection bracket below the second lifting motor. A second injection tube is connected to the second injection connecting block. A second injection screw, corresponding to the second lifting motor, is rotatably connected to the second injection bracket. The second injection connecting block is threadedly connected to the corresponding second injection screw. The second injection tube can inject the solution into the corresponding second storage tank.

[0015] As a preferred embodiment of the microfluidic detection device for food additives in this invention, it further includes two sets of horizontally movable clamping assemblies for clamping a first microfluidic chip and a second microfluidic chip, respectively. Each clamping assembly includes a clamping base plate fixed within the detection housing. A clamping support seat capable of sliding left and right is connected to the upper side of the clamping base plate. A clamping moving motor is fixedly connected to the clamping base plate. A clamping moving screw is rotatably connected to the clamping base plate, and the clamping moving screw is connected to the clamping moving motor. The clamping support seat is threaded onto the clamping moving screw. A clamping support plate is fixedly connected to the upper side of the clamping support seat. Clamping support parts are fixedly connected to the front and rear ends of the clamping support plate, respectively. A clamping motor is fixedly connected to the outer side of one clamping support part. A clamping screw is rotatably connected to the motor between two clamping supports. A clamping moving block is threaded onto the clamping screw. A connecting part is fixedly connected to the upper side of the end of the clamping moving block that extends out of the clamping support plate. A movable clamping plate that slides along the upper side of the clamping support plate is fixedly connected to the connecting part. A fixed clamping plate that is opposite to the movable clamping plate is fixedly connected to the upper side of the clamping support plate. A first detection camera is fixedly connected to a fixed frame at one end of the first injection assembly in the left-right direction. A second detection camera is fixedly connected to a fixed frame at one end of the second injection assembly in the left-right direction. The first microfluidic chip can move to below the first detection camera under the drive of the corresponding clamping seat. The second microfluidic chip can move to below the second detection camera under the drive of the corresponding clamping seat.

[0016] In a preferred embodiment of the microfluidic detection device for food additives in this invention, the first moving transmission part has a first insertion hole, the second moving transmission part has a second insertion hole, and the third moving transmission part has a third insertion hole. The first injection assembly further includes a first upper connecting plate, which is fixedly connected to one end of the first connecting bracket in the front-rear direction. A plurality of first guide rods are arranged on the first upper connecting plate, and a first lower connecting plate is fixedly connected to the lower side of each guide rod. A first drive motor is fixedly connected to the upper side of the first upper connecting plate, and a first drive screw is rotatably connected between the first upper connecting plate and the first lower connecting plate via the first drive motor. A first guide seat is threadedly connected to a first guide rod and slides along it. A first movable screw, a second movable screw, and a third movable screw are rotatably connected to the first guide seat. A first movable seat that is slidably connected to the first guide seat is threadedly connected to the first movable screw. A first movable rod that can be inserted into a first insertion hole is fixedly connected to the lower side of the first movable seat. A second movable seat that is slidably connected to the second guide seat is threadedly connected to the second movable screw. A second movable rod that can be inserted into a second insertion hole is fixedly connected to the lower side of the second movable seat. A third movable seat that is slidably connected to the third guide seat is threadedly connected to the third movable screw. A third movable rod that can be inserted into a third insertion hole is fixedly connected to the lower side of the third movable seat.

[0017] In a preferred embodiment of the microfluidic detection device for food additives in this invention, the fourth moving transmission part has a fourth insertion hole, the second injection assembly further includes a second upper connecting plate, the second upper connecting plate is fixedly connected to one end of the second connecting bracket in the front-rear direction, a plurality of second guide rods are arranged on the second upper connecting plate, a second lower connecting plate is fixedly connected to the lower side of the second guide rods, a second drive motor is fixedly connected to the upper side of the second upper connecting plate, a second drive screw is rotatably connected to the second drive motor between the second upper connecting plate and the second lower connecting plate, a second guide seat is threadedly connected to the second drive screw and slides along the second guide rod, a fourth moving screw is rotatably connected to the second guide seat, a fourth moving seat is threadedly connected to the fourth moving screw and slides on the second guide seat, and a fourth moving rod that can be inserted into the fourth insertion hole is fixedly connected to the lower side of the fourth moving seat.

[0018] Compared with existing technologies, this invention has the following technical advantages: By using a first microfluidic chip, two methods can be used to detect nitrite, improving the reliability of nitrite detection; by using a second microfluidic chip, solutions in different reservoirs can be controlled to exit according to a set reaction sequence, indirectly measuring chloramphenicol, amantadine, and Sudan I; by using a first and second injection assembly, corresponding solutions are injected into each reservoir of the first and second microfluidic chips respectively. A movable connection is established between the plug rod and the corresponding moving valve, allowing the plug rod to move the corresponding moving valve and control the solution flow. After detection using the microfluidic chip, simply move the plug rod upwards to remove it from the moving valve, facilitating the disassembly of the microfluidic chip. This invention can be applied to the detection of food additives, especially nitrite, chloramphenicol, amantadine, and Sudan I. Attached Figure Description

[0019] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Wherein:

[0020] Figure 1 This is a three-dimensional structural diagram of the first microfluidic chip in this invention.

[0021] Figure 2 This is a three-dimensional structural diagram of the first microfluidic chip during the first stage of testing.

[0022] Figure 3This is a three-dimensional structural diagram of the first microfluidic chip during the second stage of detection.

[0023] Figure 4 This is a three-dimensional structural diagram of the second microfluidic chip in this invention.

[0024] Figure 5 This diagram shows the structure of the moving valves connected in the second microfluidic chip when one end of each of the two first liquid inlets is connected to the antigen reservoir and the first antibody reservoir, respectively.

[0025] Figure 6 This is a structural diagram showing the connection of each moving valve to the second microfluidic chip when one end of the second liquid passage is connected to the clean liquid storage tank.

[0026] Figure 7 This is a structural diagram showing the connection of each moving valve to the second microfluidic chip when one end of the second liquid passage is connected to the second antibody reservoir.

[0027] Figure 8 This is a structural diagram showing the connection of each moving valve to the second microfluidic chip when one end of the second liquid passage is connected to the luminescent substrate storage tank.

[0028] Figure 9 The three-dimensional structure of the present invention (in which the detection housing is set to a transparent state). Figure 1 .

[0029] Figure 10 The three-dimensional structure of the present invention (in which the detection housing is set to a transparent state). Figure 2 .

[0030] Figure 11 for Figure 10 A magnified view of a portion of point A in the middle.

[0031] Figure 12 This is the three-dimensional structure of the invention after concealing the top cover and the detection housing. Figure 1 .

[0032] Figure 13 for Figure 12 A magnified view of a section at point B in the middle.

[0033] Figure 14 for Figure 12 A magnified view of a section at point C.

[0034] Figure 15 This is the three-dimensional structure of the invention after concealing the top cover and the detection housing. Figure 2 .

[0035] Figure 16 for Figure 15 A magnified view of a section at point D.

[0036] Figure 17This is a structural diagram showing the movement of the first movable valve, the second movable valve, and the third movable valve in the first injection assembly.

[0037] Figure 18 The structural diagram shows the implementation of the fourth moving valve in the injection assembly.

[0038] Figure 19 The three-dimensional structure of the two clamping components arranged opposite each other in this invention Figure 1 .

[0039] Figure 20 The three-dimensional structure of the two clamping components arranged opposite each other in this invention Figure 2 .

[0040] Figure 21 This is the three-dimensional structure of the invention after concealing the top cover and the detection housing. Figure 2 .

[0041] Figure 22 for Figure 21 A magnified view of a section at point E in the middle.

[0042] Figure 23 This is the three-dimensional structure of the invention after concealing the top cover and the detection housing. Figure 2 .

[0043] Figure 24 for Figure 23 A magnified view of a section at point F.

[0044] In the diagram, 100 is the first microfluidic chip, 101 is the first lower chip body, 101a is the second waste liquid tank, 101b is the first waste liquid tank, 101c is the connecting channel, 101d is the first storage tank, 101e is the first moving channel, 101f is the second moving channel, 101g is the second mixing channel, 101h is the third moving channel, 101i is the first mixing channel, 102 is the first upper chip body, 102a is the second suction port, 102b is the second upper push-pull part, 102c is the first suction port, 103 is the third moving valve, 103a is the third moving transmission part, 103a-1 is the third insertion hole, 103b is the third moving part, 103b-1 is the first connecting waste liquid hole, 103b-2 is the second connecting waste liquid hole, 104 is the second moving valve, 104a is the second moving part. Transmission unit, 104a-1 second insertion hole, 104b second moving part, 104b-1 first connecting mixing liquid hole, 105 first moving valve, 105a first moving transmission unit, 105a-1 first insertion hole, 105b first moving part, 105b-1 liquid outlet hole, 200 second microfluidic chip, 201 fourth moving valve, 201a fourth moving part, 201a-1 first liquid passage hole, 201a-2 second liquid passage hole, 201b fourth moving transmission unit, 201b-1 fourth insertion hole, 202 second lower chip body, 202a microchannel, 202b third mixing channel, 202c fourth moving channel, 202d luminescent substrate reservoir, 202e second antibody reservoir, 202f cleaning reservoir, 20 2g First antibody reservoir, 202h Antigen reservoir, 202i Third waste liquid reservoir, 203 Second upper chip body, 203a Fourth upper push-pull part, 203b Second waste liquid reservoir, 203c Second sample inlet, 203d Third upper push-pull part, 300 Support base plate, 400 Detection housing, 500 Top cover, 600 First injection assembly, 601 First connecting bracket, 700 Second injection assembly, 602 First injection bracket, 603 First lifting motor, 604 First injection screw, 605 First injection connecting block, 606 First injection tube, 607 First syringe, 608 First moving motor, 609 First guide seat, 610 First lower connecting plate, 611 First guide rod, 612 First upper connecting plate, 613 First transmission... 614 Moving screw, 616 Second moving motor, 616 Third moving rod, 617 First moving rod, 618 First moving screw, 619 Second moving screw, 620 Third moving screw, 621 Third moving motor, 622 Second moving seat, 623 First moving seat, 624 Third moving seat, 625 First peristaltic pump, 626 First liquid extraction lifting screw, 627 First liquid extraction tube, 628 Second liquid extraction tube, 629 Second liquid extraction connecting block, 630 Second liquid extraction lifting screw, 631 Second liquid extraction upright plate, 632 First liquid extraction motor, 633 First liquid extraction upright plate, 634 First liquid extraction connecting block, 635 Second liquid extraction motor, 700 Second injection assembly, 701 Second connecting bracket, 702 Second syringe.703 Second lifting motor, 704 Second injection screw, 705 Second injection connecting block, 706 Second injection fluid tube, 707 Second guide seat, 708 Fourth moving motor, 709 Second transmission motor, 710 Second upper connecting plate, 711 Second lower connecting plate, 712 Fourth moving rod, 713 Fourth moving screw, 714 Fourth moving seat, 715 Second guide rod, 716 Third peristaltic pump, 717 Third suction connecting block, 718 Third suction tube, 719 Third suction... Liquid lifting screw, 720 third liquid pumping motor, 721 third liquid pumping vertical plate, 722 second liquid pumping bracket, 800 fixed frame, 900 clamping assembly, 901 clamping motor, 902 clamping screw, 903 clamping moving block, 904 connecting part, 905 clamping support plate, 905a fixed clamping plate, 905a-1 second guide hole, 905a-2 first guide hole, 906 clamping base plate, 907 clamping support seat, 908 clamping moving screw, 909 movable clamping plate. Detailed Implementation

[0045] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0046] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0047] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that is mutually exclusive with other embodiments.

[0048] Example 1

[0049] Reference Figures 1 to 8 This embodiment provides a microfluidic detection device for food additives, which can detect different food additives.

[0050] A microfluidic detection device for food additives includes a first microfluidic chip 100, a first movable valve 105, a second movable valve 104, a third movable valve 103, a first upper chip body 102, and a first lower chip body 101 fixed below the first upper chip body 102. The left and right sides of the upper end of the first upper chip body 102 are respectively fixed with a first upper push-pull portion and a second upper push-pull portion 102b for convenient adjustment of the left-right position of the first microfluidic chip 100. The upper end of the first lower chip body 101 has a plurality of first liquid reservoirs 101d and a plurality of outlets corresponding to the first liquid reservoirs 101d. The system includes a liquid flow channel, a first mixing flow channel 101i, a second mixing flow channel 101g, a first waste liquid tank 101b, and a second waste liquid tank 203b101a. One end of the liquid outlet channel is connected to the first storage tank 101d, and the other end of the liquid outlet channel is controllably connected to one end of the first mixing flow channel 101i or one end of the second mixing flow channel 101g. The other end of the second mixing flow channel 101g is connected to the second waste liquid tank 203b101a. A first moving channel 101e is opened at the upward-facing end of the first lower chip body 101 between the liquid outlet channels and the first mixing flow channel 101i or the second mixing flow channel 101g. A first moving valve is also included. The second moving valve 104 can slide along the first moving channel 101e. A connecting channel 101c is provided on the first lower chip body 101 between the first mixing channel 101i and the first waste liquid pool 101b. A second moving channel 101f is opened at the upper end of the first lower chip body 101 between the beginning of the connecting channel 101c and the end of the first mixing channel 101i. The second moving valve 104 can slide along the second moving channel 101f. A second moving channel 104 is opened on the first lower chip body 101 between the first mixing channel 101i and the first waste liquid pool 101b, and between the second mixing channel 101g and the second waste liquid pool 203b101a. The third moving channel 101h, the third moving valve 103 can slide along the third moving channel 101h, the first moving valve 105 enables several liquid storage tanks to be controllably connected to the first mixing channel 101i or the second mixing channel 101g, the second moving valve 104 enables the first mixing channel 101i to be connected to the first waste liquid tank 101b or the other end of the first mixing channel 101i to be connected to the second mixing channel 101g, the third moving valve 103 enables the other end of the first mixing channel 101i to be connected to the first waste liquid tank 101b or the end of the second mixing channel 101g to be connected to the second waste liquid tank 203b101a;

[0051] The second microfluidic chip 200 includes a fourth movable valve 201, a second upper chip body 203, and a second lower chip body 202 fixed below the second upper chip body 203. The upper left and right sides of the second upper chip body 203 are respectively fixed with a third upper push-pull portion 203d and a fourth upper push-pull portion 203a for convenient adjustment of the left-right position of the second microfluidic chip 200. The upper end of the second lower chip body 202 has an antigen reservoir 202h, a first antibody reservoir 202g, a cleaning reservoir 202f, a second antibody reservoir 202e, a luminescent substrate reservoir 202d, and a third mixing channel. 202b, antigen reservoir 202h, first antibody reservoir 202g, cleaning reservoir 202f, second antibody reservoir 202e, luminescent substrate reservoir 202d are controllably connected to one end of the third mixing channel 202b. At the other end of the third mixing channel 202b, a microchannel 202a is opened at the upward-facing end of the second lower chip body 202. A silicone membrane coated with antigens (three vertical lines, representing antigens used to detect three different hazardous substances) is sandwiched between the second upper chip body 203 and the second lower chip body 202 at the microchannel 202a. The microchannel 202a is located away from the second lower chip body 202b of the third mixing channel 202b. A third waste liquid pool 202i is opened at the upward-facing end of the chip body 202. A fourth moving channel 202c is opened on the second lower chip body 202 between the liquid storage pool and the third mixing channel 202b. The fourth moving valve 201 includes a fourth moving transmission part 201b outside the second lower chip body 202. The fourth moving transmission part 201b has a fourth insertion hole 201b-1. A fourth moving part 201a is fixed on the fourth moving transmission part 201b, which is inserted into the fourth moving channel 202c. The fourth moving part 201a has two first liquid passage holes 201a-1 and one second liquid passage hole 201a-2. When one end of each of the two first liquid inlets 201a-1 is connected to the antigen reservoir 202h and the first antibody reservoir 202g respectively, the other end of the first liquid inlet 201a-1 is connected to one end of the third mixing channel 202b, and the second liquid inlet 201a-2 is staggered from any other reservoir; when one end of the second liquid inlet 201a-2 is connected to the clean reservoir 202f or the second antibody reservoir 202e or the luminescent substrate reservoir 202d, the other end of the second liquid inlet 201a-2 is connected to one end of the third mixing channel 202b, and the two first liquid inlets 201a-1 are staggered from any other reservoir.

[0052] The second microfluidic chip 200 was used to detect chloramphenicol, amantadine, and Sudan I. The specific detection process was as follows: chloramphenicol, amantadine, and Sudan I were injected into the antigen reservoir 202h; monoclonal antibodies against chloramphenicol, amantadine, and Sudan I were injected into the first antibody reservoir 202g; PBST solution was injected into the clean reservoir 202f; HRP-labeled goat anti-mouse IgG was injected into the second antibody reservoir 202e; and luminol, a chemiluminescent substrate, was injected into the luminescent substrate reservoir 202d. Initially, the fourth moving valve 201 ensured that each reservoir was uniformly mixed with the third... The mixing channel 202b is isolated. During testing, peristaltic pumps are connected to the third waste tank 202i. The fourth moving valve 201 is moved to simultaneously connect the antigen reservoir 202h and the first antibody reservoir 202g to the third mixing channel 202b. The fourth moving valve 201 stops moving, and the peristaltic pumps are activated, allowing the analyte and excess antibody to enter the third mixing channel 202b for mixing. The analyte then settles in the wave-shaped microchannel 202a on the reaction layer for incubation (37°C for 15 minutes). After incubation, the fourth moving valve 201 is moved to allow the clean reservoir 202h to be isolated. 02f and the third mixing channel 202b are connected. The fourth moving valve 201 stops moving, and PBST in the cleaning reservoir 202f is extracted to clean the reaction layer. After cleaning, the peristaltic pump stops operating. The fourth moving valve 201 is then moved to connect the second antibody reservoir 202e and the third mixing channel 202b. The fourth moving valve 201 stops moving, and the peristaltic pump operates. HRP-labeled goat anti-mouse IgG is extracted into microchannel 202a to react with the antigen. The peristaltic pump is then controlled to keep the antibody stationary in microchannel 202a for incubation (10 minutes at room temperature). A second incubation is then performed. Afterwards, the peristaltic pump stops operating, and the fourth moving valve 201 is moved to connect the cleaning reservoir 202f and the third mixing channel 202b. The fourth moving valve 201 stops moving, and PBST in the cleaning reservoir 202f is extracted to clean the reaction layer. The peristaltic pump stops operating, and the cleaning is completed. The fourth moving valve 201 is then moved to connect the luminescent substrate reservoir 202d and the third mixing channel 202b. The peristaltic pump operates, and the chemiluminescent substrate is pumped out, generating a chemiluminescent signal (dot matrix) (nine light spots) on the reaction layer (microchannel 202a) to achieve the detection of three hazardous substances.

[0053] In this application, different competing antigens are arranged and fixed on the silicone membrane sandwiched in the second microfluidic chip 200, and the spatial resolution acquisition of chemiluminescence signals is combined to achieve simultaneous detection of three hazardous substances. A single detection can output three sets of independent quantitative data.

[0054] Specifically, the first moving valve 105 includes a first moving transmission part 105a outside the first lower chip body 101. A first moving part 105b, which is inserted into the first moving channel 101e, is fixed on the first moving transmission part 105a. A plurality of first liquid storage pools 101d are arranged with corresponding outlet holes 105b-1. There are three first liquid storage pools 101d. When one end of two outlet holes 105b-1 is connected to two of the first liquid storage pools 101d, the other end of the two outlet holes 105b-1 is connected to the first mixing channel 101i. When one end of one of the three outlet holes 105b-1 is connected to another, the other end of the two outlet holes 105b-1 is connected to the first mixing channel 101i. When the end is connected to the three first liquid storage tanks 101d one by one, the other ends of two of the liquid outlet holes 105b-1 are connected to the first mixing channel 101i, and the other end of the other liquid outlet hole 105b-1 is connected to the first end of the second mixing channel 101g; the second moving valve 104 includes a second moving transmission part 104a outside the first lower chip body 101, and a second moving part 104b that is just inserted into the second moving channel 101f is fixed on the second moving transmission part 104a. The second moving part 104b has a first connecting mixing liquid hole 104b-1 and a second connecting mixing liquid hole, and one end of the first connecting mixing liquid hole 104b-1 is connected to the first mixing liquid hole 104b-1. When connected to the end of the first mixing channel 101i, the other end of the first connecting mixing liquid hole 104b-1 is connected to the first waste liquid pool 101b. The second connecting mixing liquid hole is offset from any mixing channel or waste liquid pool. When one end of the second connecting mixing liquid hole is connected to the end of the first mixing channel 101i, the other end of the second connecting mixing liquid hole is connected to the second mixing channel 101g. The third moving valve 103 includes a third moving transmission part 103a outside the first lower chip body 101. A third moving part 103b is fixed on the third moving transmission part 103a and is inserted into the third moving channel 101h. The device has a first waste liquid connection hole 103b-1 and a second waste liquid connection hole 103b-2. When the two ends of the first waste liquid connection hole 103b-1 are connected to the first mixing channel 101i and the first waste liquid pool 101b respectively, the two ends of the second waste liquid connection hole 103b-2 are staggered from the second mixing channel 101g and the second waste liquid pool 203b101a respectively. When the two ends of the second waste liquid connection hole 103b-2 are connected to the second mixing channel 101g and the second waste liquid pool 203b101a respectively, the two ends of the first waste liquid connection hole 103b-1 are staggered from the second mixing channel 101g and the second waste liquid pool 203b101a respectively.

[0055] Reference Figure 2 and Figure 3The three first storage tanks 101d are respectively filled with a mixed solution of nitrite and hydrochloric acid, a potassium ferrocyanide solution (colorless), and a mixed solution of uric acid, luminol, and sodium hydroxide. The first stage detection process is as follows: the first moving valve 105, the second moving valve 104, and the third moving valve 103 are moved. The movement of the first moving valve 105 connects the two left storage tanks and the first mixing channel 101i. When the first moving valve 105 stops moving, the mixed solution of nitrite and hydrochloric acid and the potassium ferrocyanide solution enter the first mixing channel 101i. A mixing reaction occurs within the mixing channel 101i, where nitrite oxidizes potassium ferrocyanide to potassium ferricyanide. The solution changes from colorless before mixing to yellow. Movement of the second moving valve 104 and the third moving valve 103 connects the first mixing channel 101i to the beginning of the connecting channel 101c and the end of the connecting channel 101c to the first waste liquid tank 101b. The second moving valve 104 and the third moving valve 103 then stop moving, and the reacted solution enters the first waste liquid tank 101b. The change in yellow color reflects the nitrite's reaction. Preliminary testing to determine the presence of nitrite; the second stage of testing begins, and the process is as follows: The first moving valve 105, the second moving valve 104, and the third moving valve 103 are moved, connecting the two first storage tanks 101d on the left and the first mixing channel 101i, and connecting the first outlet tank and the second mixing channel 101g on the right. The second moving valve 104 connects the end of the first mixing channel 101i with the second mixing channel 101g, and the end of the second mixing channel 101g with the second waste tank 2. When 03b101a is connected, the first moving valve 105 stops moving, the second moving valve 104 stops moving, and the third moving valve 103 stops moving, so that the potassium ferricyanide generated after the reaction in the two first liquid storage tanks 101d on the left mixes with the solution in the first liquid storage tank 101d on the far right. Under the enhanced effect of uric acid, potassium ferricyanide oxidizes luminol to produce chemiluminescence, thereby indirectly determining nitrite. The first microfluidic chip 100 realizes two methods to determine nitrite, improving the reliability of nitrite detection.

[0056] When using the first microfluidic chip 100 for detection, the following two methods are employed:

[0057] First, the colorimetric method: based on the redox reaction of nitrite and potassium ferrocyanide in an acidic medium (producing yellow potassium ferrocyanide), quantitative analysis is performed by changing the color of the solution (absorbance). Its advantages are its intuitiveness and rapid qualitative judgment.

[0058] Second, chemiluminescence method: chemiluminescence is generated by potassium ferricyanide and luminol under the enhancement effect of uric acid. The intensity of the light signal is used for quantification. Its advantages are ultra-high sensitivity and resistance to background interference.

[0059] Complementary effects: Colorimetric methods provide preliminary qualitative results (such as color mutations indicating exceeding the standard), while chemiluminescence methods provide precise quantification. The combination of the two covers the entire process from "coarse screening" to "precise testing".

[0060] The above method achieves cross-validation of nitrite concentration through dual-modal detection of colorimetric reaction (change in yellowness of solution) and chemiluminescence signal, greatly improving the accuracy of detection. The potassium ferrocyanide reaction zone and luminol luminescence reaction zone are integrated in the microchannel, and the spatiotemporal separation of the two-stage reaction is achieved by valve control to avoid mutual interference of reactants.

[0061] Example 2

[0062] Reference Figures 9 to 24 This embodiment provides a microfluidic detection device for food additives, which can further realize the injection of solutions into each storage tank and the control of the reaction process.

[0063] Specifically, it also includes a detection housing 400, with a supporting base plate 300 fixedly connected to the lower end of the detection housing 400. A fixing frame 800 is fixedly connected to the upper side of the supporting base plate 300, and a horizontally movable seat is connected to the fixing frame 800. The detection housing 400 is equipped with a first injection assembly 600 for injecting solution into the first microfluidic chip 100 and detecting the food additive to be tested, and a second injection assembly 700 for injecting solution into each reservoir in the second microfluidic chip 200 and detecting the food additive to be tested. A top cover 500 is fixedly connected to the upper end of the detection housing 400. The first injection assembly 600 includes a first connector connected to the movable seat. A bracket 601 and several first syringes 607, each corresponding to a first liquid storage tank 101d, are connected to the lower end of the top cover 500. Several first injection brackets 602 are connected to the first injection brackets 602, and several first lifting motors 603 are fixedly connected to each first injection bracket 602. First injection connecting blocks 605 are slidably connected to the first injection brackets 602 below the first lifting motors 603. First injection tubing 606 is connected to the first injection connecting blocks 605. The end of each first syringe 607 is connected to the first injection tubing 606 via a flexible conduit. When the first syringe 607 is activated, it pumps solution into the corresponding first injection tubing 606. The injection support 602 is rotatably connected to a first injection screw 604, which corresponds one-to-one with the first lifting motor 603. A first injection connecting block 605 is threadedly connected to the corresponding first injection screw 604. The first injection tube 606 can inject the solution into the corresponding first reservoir 101d. The second injection assembly 700 includes a second connecting bracket 701 connected to a movable base and a plurality of second syringes 702 connected to the detection housing 400. The plurality of second syringes 702 correspond one-to-one with the plurality of reservoirs in the second microfluidic chip 200. A plurality of second injection supports are connected to the second connecting bracket 701, and a plurality of second injection supports are fixedly connected to the second injection supports. A second lifting motor 703 is used. A second injection connecting block 705 is slidably connected to a second injection bracket below the second lifting motor 703. A second injection liquid tube 706 is connected to the second injection connecting block 705. The end of the second syringe 702 is connected to the second injection liquid tube 706 via a flexible tube. When the second syringe 702 is activated, it pumps the solution into the corresponding second injection liquid tube 706. A second injection screw 704, corresponding to the second lifting motor 703, is rotatably connected to the second injection bracket. The second injection connecting block 705 is threaded onto the corresponding second injection screw 704. The second injection liquid tube 706 can inject the solution into the corresponding second storage tank.

[0064] Each first syringe 607 and each second syringe 702 stores the required analyte or detection solution. When the required solution needs to be injected into each of the first reservoirs 101d of the first microfluidic chip 100, the first lifting motor 603 is activated, the first injection screw 604 rotates, the first injection screw 604 drives the first injection connector 605 to move downward, the first injection connector 605 drives the first injection tube 606 to move downward. When the injection needle at the lower end of the first injection tube 606 is inserted into the corresponding first inlet port on the first upper chip body 102, the first lifting motor 603 stops. When the solution injected into each of the first reservoirs 101d reaches the required volume, the first syringe 607 stops, the first lifting motor 603 reverses its operation, causing the first injection connector 605 to move upward to its initial position. 3. Stop the operation to achieve sample injection into the first microfluidic chip 100; when the required solution needs to be injected into each reservoir of the second microfluidic chip 200, the second lifting motor 703 is activated, the second injection screw 704 rotates, the second injection screw 704 drives the second injection connecting block 705 to move down, the second injection connecting block 705 drives the second injection tube 706 to move down, when the injection needle at the lower end of the second injection tube 706 is inserted into the corresponding second sample inlet 203c on the second upper chip body 203, the second lifting motor 703 stops the operation. When the solution injected into each second reservoir reaches the required volume, the second syringe 702 stops the operation, the second lifting motor 703 reverses the operation, causing the second injection connecting block 705 to move up to the initial position, the second lifting motor 703 stops the operation, and the sample injection into the second microfluidic chip 200 is achieved.

[0065] A first extraction hole 102c and a second extraction hole 102a are respectively opened on the first chip body 102 at the first waste liquid pool 101b and the second waste liquid pool 203b101a. The first injection assembly 600 also includes a first peristaltic pump 625 and a second peristaltic pump fixedly connected in the detection housing 400. A first hose and a second hose are respectively connected to the first peristaltic pump 625 and the second peristaltic pump. A first extraction bracket is fixedly connected to the first connecting bracket 601. A first extraction plate 633 and a second extraction plate 631 are connected to the first extraction bracket. A first extraction motor 632 is fixedly connected to the first extraction plate 633. A first extraction lifting screw is rotatably connected to the first extraction plate 633 below the first extraction motor 632. 626, a first liquid-drawing lifting screw 626 is threadedly connected to a first liquid-drawing connecting block 634 that is slidably connected to a first liquid-drawing vertical plate 633. A first liquid-drawing pipe 627 is fixedly connected to the first liquid-drawing connecting block 634. The end of the first liquid-drawing pipe 627 away from the first liquid-drawing connecting block 634 is connected to a first flexible hose. A second liquid-drawing motor 635 is fixedly connected to a second liquid-drawing vertical plate 631. A second liquid-drawing lifting screw 630 is rotatably connected to the second liquid-drawing vertical plate 631 below the second liquid-drawing motor 635. A second liquid-drawing lifting screw 630 is threadedly connected to a second liquid-drawing connecting block 629 that is slidably connected to a second liquid-drawing vertical plate 631. A second liquid-drawing pipe 628 is fixedly connected to the second liquid-drawing connecting block 629. 8. When the end furthest from the first liquid extraction connecting block 634 is connected to the second tubing, and the solution in the storage tank of the first microfluidic chip 100 needs to flow towards the first waste liquid tank 101b, the first liquid extraction motor 632 is activated, the first liquid extraction lifting screw 626 rotates, causing the first liquid extraction connecting block 634 to move downwards, and the tip of the bottom of the first liquid extraction tube 627 is just inserted into the first liquid extraction hole 102c. The first liquid extraction motor 632 stops operating, and the first peristaltic pump 625 is activated, causing the solution in the two first storage tanks 101d on the left to flow towards the first waste liquid tank 101b through the first waste liquid tank 101b. After the reacted solution enters the first waste liquid tank 101b, the first peristaltic pump 625 stops operating, and the first liquid extraction motor 632... 2. Reverse action: When the first suction tube 627 is lifted away from the second microfluidic chip 200 to the initial height, the first suction motor 632 stops. When it is necessary for the solution in the three first liquid storage tanks 101d in the first microfluidic chip 100 to flow in the direction of the second waste liquid tank 203b101a, the second suction motor 635 is activated, the second suction lifting screw 630 rotates, driving the second suction connecting block 629 to move down, and the tip of the bottom of the second suction tube 628 is just inserted into the second suction hole 102a. The second suction motor 635 stops, the second peristaltic pump is activated, and the solution in the three first liquid storage tanks 101d on the left flows in the direction of the second waste liquid tank 203b101a through the second waste liquid tank 203b101a.After the reacted solution enters the second waste liquid tank 203b101a, the second peristaltic pump stops operating, and the second pumping motor 635 reverses its operation. When the second pumping tube 628 is lifted away from the second microfluidic chip 200 to its initial height, the second pumping motor 635 stops operating. When the solution flows to the required position and needs to be held for a period of time, the corresponding peristaltic pump is controlled to operate. This is existing technology and will not be elaborated further.

[0066] A third liquid extraction hole is opened on the second upper chip body 203 at the third waste liquid pool 202i. The second injection assembly 700 also includes a third peristaltic pump 716 fixedly connected in the detection housing 400. A third hose is connected to the third peristaltic pump 716. A second liquid extraction bracket 722 is fixedly connected to the second connecting bracket 701. A third liquid extraction stand 721 is connected to the second liquid extraction bracket 722. A third liquid extraction motor 720 is fixedly connected to the third liquid extraction stand 721 below the third liquid extraction motor 720. A third liquid extraction lifting screw 719 is rotatably connected to the third liquid extraction lifting screw 719. A third liquid extraction connecting block 717 is threadedly connected to the third liquid extraction stand 721. A third liquid extraction tube 718 that can be aligned with the third liquid extraction hole is fixedly connected to the third liquid extraction connecting block 717. The end of the third liquid extraction tube 718 away from the third liquid extraction connecting block 717 is connected to the third hose.

[0067] When it is necessary for the solution at the end of the storage tank to flow towards the third waste liquid tank 202i, the third pumping motor 720 is activated, the third pumping lifting screw 719 rotates, driving the third pumping connecting block 717 to move. The direction of the third pumping motor 720 is controlled, causing the third pumping connecting block 717 to move downward. When the tip of the bottom of the third pumping pipe 718 is just inserted into the third pumping hole, the third pumping motor 720 stops, and the third peristaltic pump 716 is activated, causing the solution in the storage tank to flow towards the third waste liquid tank 202i. When it is necessary for the solution to remain in a certain channel for reaction, the action of the peristaltic pump is controlled to make the mixed reaction solution remain in the set channel for reaction.

[0068] To further achieve the clamping of the first microfluidic chip 100 and the second microfluidic chip 200, two sets of horizontally movable clamping assemblies 900 are also included, each used to clamp the first microfluidic chip 100 and the second microfluidic chip 200 respectively. Each clamping assembly 900 includes a clamping base plate 906 fixed within the detection housing 400. A clamping support seat 907 capable of sliding left and right is connected to the upper side of the clamping base plate 906. A clamping moving motor is fixedly connected to the clamping base plate 906. A clamping moving screw 908 is rotatably connected to the clamping base plate 906 and is connected to the clamping moving motor. The clamping support seat 907 is threadedly connected to the clamping moving screw 908. A clamping support plate 905 is fixedly connected to the upper side of the clamping support seat 907. Clamping support parts are fixedly connected to the front and rear ends of the clamping support plate 905 respectively. A clamping motor 901 is fixedly connected to the outer side of one clamping support part. A clamping screw 902 is rotatably connected between two clamping supports. A clamping moving block 903 is threaded onto the clamping screw 902. A connecting part 904 is fixedly connected to the upper side of the end of the clamping moving block 903 that extends out of the clamping support plate 905. A movable clamping plate 909 that slides along the upper side of the clamping support plate 905 is fixedly connected to the connecting part 904. A fixed clamping plate 905a that is opposite to the movable clamping plate 909 is fixedly connected to the upper side of the clamping support plate 905. A first detection camera is fixedly connected to the fixing frame 800 at one end of the first injection assembly 600 in the left-right direction. A second detection camera is fixedly connected to the fixing frame 800 at one end of the second injection assembly 700 in the left-right direction. The first microfluidic chip 100 can move to below the first detection camera under the drive of the corresponding clamping seat. The second microfluidic chip 200 can move to below the second detection camera under the drive of the corresponding clamping seat.

[0069] Taking the clamping of the first microfluidic chip 100 as an example, the clamping process is explained as follows: The first microfluidic chip 100 is placed on the corresponding clamping support plate 905, with the rear end of the first microfluidic chip 100 attached to the front side of the fixed clamping plate 905a. The position of the first microfluidic chip 100 in the left-right direction is adjusted so that the first liquid extraction hole 102c is aligned with the first liquid extraction tube 627 and the second liquid extraction hole 102a is aligned with the second liquid extraction tube 628. The clamping motor 901 is activated, the clamping screw 902 rotates, and the clamping moving block 903 slides, driving the movable clamping plate 909 to move via the connecting part 904. The direction of action of the clamping motor 901 is controlled so that the movable clamping plate 909 moves in the direction of the first microfluidic chip 100. When the movable clamping plate 909 touches the front side of the first microfluidic chip 100, the clamping motor 901 stops, thus achieving the clamping and fixing of the first microfluidic chip 100.

[0070] The clamping and fixing process of the second microfluidic chip 200 is similar to that of the first microfluidic chip 100, and will not be described in detail here.

[0071] Specifically, the first moving transmission part 105a has a first insertion hole 105a-1, the second moving transmission part 104a has a second insertion hole 104a-1, and the third moving transmission part 103a has a third insertion hole 103a-1. The first injection assembly 600 also includes a first upper connecting plate 612, which is fixedly connected to one end of the first connecting bracket 601 in the front-rear direction. A plurality of first guide rods 611 are arranged on the first upper connecting plate 612, and the lower side of the first guide rods 611... A first lower connecting plate 610 is fixedly connected. A first drive motor is fixedly connected to the upper side of a first upper connecting plate 612. A first drive screw 613 is rotatably connected between the first upper connecting plate 612 and the first lower connecting plate 610 connected to the first drive motor. A first guide seat 609, which slides along a first guide rod 611, is threaded onto the first drive screw 613. A first moving screw 618, a second moving screw 619, and a third moving screw 620 are rotatably connected to the first guide seat 609. A first movable seat 623, which is slidably connected to a first guide seat 609, is threaded onto the second movable screw 619. A first movable rod 617, which can be inserted into a first insertion hole 105a-1, is fixedly connected to the lower side of the first movable seat 623. A second movable seat 622, which can be inserted into a second insertion hole 104a-1, is threaded onto the second movable screw 619. A second movable seat 622, which is slidably connected to a second guide seat 607, is threaded onto the lower side of the second movable seat 622. A second movable rod 616, which can be inserted into a second insertion hole 104a-1, is fixedly connected to the lower side of the second movable seat 622. A third movable screw 620, which is threaded onto the third movable screw 619, is slidably connected to a first movable seat 622, which is slidably connected to a first guide seat 609. The third movable seat 624 on the three guide seats has a third movable rod 616 fixedly connected to its lower side, which can be inserted into the third insertion hole 103a-1. The first movable motor 608, the second movable motor 614 and the third movable motor 621 are fixedly connected to the outer side of the first guide seat 609. The first movable screw 618 is driven by the first movable motor 608, the second movable screw 619 is driven by the second movable motor 614, and the third movable screw 620 is driven by the third movable motor 621.

[0072] After the first microfluidic chip 100 is clamped and fixed, in the default initial state, each moving rod is aligned with the corresponding insertion hole position. After the first microfluidic chip 100 is clamped and fixed, the position of each moving rod is adjusted so that each moving rod is aligned with the corresponding insertion hole. Then, each moving rod is moved down and inserted into the corresponding insertion hole to realize the preparation work for sample injection control. The specific action process is as follows: the first drive motor is activated, the first drive screw 613 rotates, the first guide seat 609 slides in the height direction, driving the first moving rod 617, the second moving rod 616 and the third moving rod 616 to move, controlling the direction of the first drive motor to move the three moving rods down. After the first moving rod 617, the second moving rod 616, and the third moving rod 616 are respectively inserted into the first insertion hole 105a-1, the second insertion hole 104a-1, and the third insertion hole 103a-1 to set their positions, the first drive motor stops operating. Taking the adjustment of the positions of the first moving valve 105, the second moving valve 104, and the third moving valve 103 as an example to illustrate solution flow control, when it is necessary for one end of two of the outlet holes 105b-1 to be connected to two of the first storage tanks 101d, the first moving motor 608 is activated, the first moving screw 618 rotates, driving the first moving seat 623 to move, which in turn drives the first moving rod 617 to move the first... When the movable valve 105 moves, and one end of each of the two outlet holes 105b-1 connects to the two first storage tanks 101d on the left, the first movable motor 608 stops operating, and the other end of each of the two outlet holes 105b-1 connects to the first mixing channel 101i. The second movable motor 614 is then activated, causing the second movable screw 619 to rotate, which in turn moves the second movable seat 622. This movement, via the second movable rod 616, moves the second movable valve 104. When one end of the first connecting mixing hole 104b-1 connects to the end of the first mixing channel 101i, the second movable motor 614 stops operating, and the other end of the first connecting mixing hole 104b-1 connects to the end of the first mixing channel 101i. When channel 101c is connected, the third moving motor 621 is activated, the third moving screw 620 rotates, driving the third moving seat 624 to move, which in turn drives the third moving valve 103 to move via the third moving rod 616. When the two ends of the first waste liquid connection hole 103b-1 are connected to the connecting channel 101c and the first waste liquid pool 101b respectively, the third moving motor 621 stops operating, realizing the controllable connection between the two first liquid storage pools 101d and the first waste liquid pool 101b on the left. During the second stage of detection, the first moving motor 608 is activated, connecting the three liquid outlet holes 105b-1 and the three first liquid storage pools 101d, and the first moving motor 608 stops operating.The second moving motor 614 is activated, causing the second moving valve 104 to move. When both ends of the second connecting mixing liquid hole are connected to the end of the first mixing channel 101i and the second mixing channel 101g respectively, the second moving motor 614 stops operating. The third moving motor 621 is then activated, causing the third moving valve 103 to move. When both ends of the second connecting waste liquid hole 103b-2 are connected to the end of the second mixing channel 101g and the second waste liquid pool 203b101a respectively, the third moving motor 621 stops operating, thus achieving controllable sample injection for both detection methods.

[0073] Specifically, the fourth moving transmission part 201b has a fourth insertion hole 201b-1. The second injection assembly 700 also includes a second upper connecting plate 710, which is fixedly connected to one end of the second connecting bracket 701 in the front-rear direction. A plurality of second guide rods 715 are arranged on the second upper connecting plate 710. A second lower connecting plate 711 is fixedly connected to the lower side of the second guide rods 715. A second drive motor 709 is fixedly connected to the upper side of the second upper connecting plate 710. The second drive motor 709 is connected to a component that is rotatably connected to the second upper connecting plate 710 and the second lower connecting plate 711. The second transmission screw in room 11 is threadedly connected to a second guide seat 707 that slides along the second guide rod 715. A fourth moving screw 713 is rotatably connected to the second guide seat 707. A fourth moving motor 708 is fixedly connected to the outside of the second guide seat 707. The fourth moving motor 708 is connected to the fourth moving screw 713. A fourth moving seat 714 that slides on the second guide seat 707 is threadedly connected to the fourth moving screw 713. A fourth moving rod 712 that can be inserted into the fourth insertion hole 201b-1 is fixedly connected to the lower side of the fourth moving seat 714.

[0074] After the second microfluidic chip 200 is clamped and fixed, in the default initial state, the fourth moving rod 712 is aligned with the position of the fourth insertion hole 201b-1. After the second microfluidic chip 200 is clamped and fixed, the position of the fourth moving rod 712 is adjusted so that the fourth moving rod 712 is aligned with the fourth insertion hole 201b-1. Then, the fourth moving rod 712 is moved down and inserted into the fourth insertion hole 201b-1 to realize the preparation work for sample injection control. The specific action process is as follows: the second drive motor 709 is activated, the second drive screw rotates, and the second drive screw drives the second guide seat 707 to move in the height direction. 07 drives the fourth moving rod 712 to move, controlling the direction of the second drive motor 709, causing the second guide seat 707 to move downwards. When the fourth moving rod 712 is inserted into the fourth moving transmission part 201b through the fourth insertion hole 201b-1, the second drive motor 709 stops operating. During detection, the fourth moving motor 708 operates, the fourth moving screw 713 rotates, driving the fourth moving seat 714 to move horizontally, which in turn drives the fourth moving valve 201 to move via the fourth moving rod 712. When one end of each of the two first liquid passage holes 201a-1 is connected to the antigen storage tank 202h and the first antibody storage tank 202g respectively, the fourth moving... When motor 708 stops operating and the antigen-antibody mixing reaction is complete, the fourth moving motor 708 is activated. When both ends of the second liquid inlet 201a-2 are connected to the cleaning reservoir 202f and the beginning of the third mixing channel 202b respectively, the fourth moving motor 708 stops operating. PBST is extracted from the cleaning reservoir 202f to clean the reaction layer. After cleaning, the fourth moving motor 708 is activated, and the fourth moving valve 201 moves. When both ends of the second liquid inlet 201a-2 are connected to the second antibody reservoir 202e and the beginning of the third mixing channel 202b respectively, the fourth moving motor 708 stops operating. After incubation, the fourth moving motor 708 is activated. When the two ends of the second liquid passage 201a-2 are connected to the cleaning reservoir 202f and the first end of the third mixing channel 202b respectively, the fourth moving motor 708 stops. PBST is extracted from the cleaning reservoir 202f to clean the reaction layer. After cleaning, the fourth moving motor 708 is activated, and the fourth moving valve 201 moves. When the two ends of the second liquid passage 201a-2 are connected to the luminescent substrate reservoir 202d and the third mixing channel 202b respectively, the fourth moving motor 708 stops, realizing the controllable flow injection of the analyte and the detection solution.

[0075] When the solution reaction inside the microfluidic chip is complete and the content of the analyte needs to be detected, the clamping moving motor is controlled to move, the clamping moving screw 908 rotates, and the clamping moving screw 908 drives the clamping support 907 to slide left and right along the clamping base plate 906. The clamping support 907 drives the corresponding microfluidic chip to move via the clamping support plate 905. When the waste liquid pool moves to the bottom of the corresponding detection camera, the clamping moving motor stops moving.

[0076] The fixing plate 905a corresponding to the first microfluidic chip 100 and the fixing plate 905a corresponding to the second microfluidic chip 200 are respectively referred to as the first fixing plate 905a and the second fixing plate 905a. The first fixing plate 905a has three first guide holes 905a-2 that correspond one-to-one with the first moving transmission part 105a, the second moving transmission part 104a and the third moving transmission part 103a. The first moving transmission part 105a, the second moving transmission part 104a and the third moving transmission part 103a are slidably connected to the first fixing plate 905a through the first guide holes 905a-2. Several insertion holes are always behind the fixing plate 905a. The second fixing plate 905a has a second guide hole 905a-1 that corresponds to the fourth moving transmission part 201b. The fourth moving transmission part 201b is slidably connected to the second fixing plate 905a through the second guide hole 905a-1.

[0077] The above structure improves the stability of each moving valve during position adjustment.

[0078] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A microfluidic detection device for food additives, characterized by: It comprises, The first microfluidic chip comprises a first upper chip body, a first lower chip body fixed on the lower side of the first upper chip body, a first moving valve, a second moving valve and a third moving valve. The upward one end of the first lower chip body is arranged with a plurality of first liquid storage pools, a plurality of liquid outlet channels corresponding to the first liquid storage pools, a first mixing channel, a second mixing channel, a first waste liquid pool and a second waste liquid pool. One end of the liquid outlet channel is connected with the first liquid storage pool, and the other end of the liquid outlet channel is controllably communicated with one end of the first mixing channel or one end of the second mixing channel. The other end of the second mixing channel is communicated with the second waste liquid pool. The upward one end of the first lower chip body between the plurality of liquid outlet channels and the first mixing channel or the second mixing channel is provided with a first moving channel. The first moving valve can slide along the first moving channel. The first lower chip body between the first mixing channel and the first waste liquid pool is provided with a second moving channel. The second moving valve can slide along the second moving channel. The first lower chip body between the first mixing channel and the first waste liquid pool and between the second mixing channel and the second waste liquid pool is provided with a third moving channel. The third moving valve can slide along the third moving channel. The first moving valve can controllably communicate the plurality of liquid storage pools with the first mixing channel or the second mixing channel. The second moving valve can communicate the first mixing channel with the first waste liquid pool or the other end of the first mixing channel with the second mixing channel. The third moving valve can communicate the other end of the first mixing channel with the first waste liquid pool or the end of the second mixing channel with the second waste liquid pool. The second microfluidic chip comprises a second upper chip body and a second lower chip body fixed on the lower side of the second upper chip body. The upward one end of the second lower chip body is provided with an antigen storage pool, a first antibody storage pool, a cleaning storage pool, a second antibody storage pool, a luminescent substrate storage pool and a third mixing channel. The antigen storage pool, the first antibody storage pool, the cleaning storage pool, the second antibody storage pool and the luminescent substrate storage pool are controllably communicated with one end of the third mixing channel. The upward one end of the second lower chip body at the other end of the third mixing channel is provided with a microchannel. The second upper chip body and the second lower chip body at the microchannel are clamped with a silica gel membrane coated with an antigen. The upward one end of the second lower chip body away from the third mixing channel is provided with a third waste liquid pool.

2. The microfluidic detection device for food additives according to claim 1, wherein: The first moving valve comprises a first moving transmission part outside the first lower chip body. A first moving part that is just inserted into the first moving channel is fixed on the first moving transmission part. A plurality of liquid outlet holes corresponding to the first liquid storage pools are arranged on the first moving part. The first liquid storage pools are provided with three. When one end of two liquid outlet holes is communicated with two first liquid storage pools, the other end of the two liquid outlet holes is communicated with the first mixing channel. When one end of three liquid outlet holes is communicated with three first liquid storage pools one by one, the other end of two liquid outlet holes is communicated with the first mixing channel, and the other end of the other liquid outlet hole is communicated with the first end of the second mixing channel.

3. The microfluidic device for the detection of food additives according to claim 2, characterized in that: The second mobile valve comprises a second mobile transmission part outside the first lower chip body, a second mobile part is fixed on the second mobile transmission part and inserted into the second mobile channel, a first connecting mixed liquid hole and a second connecting mixed liquid hole are opened on the second mobile part, one end of the first connecting mixed liquid hole is communicated with the end of the first mixed flow channel, the other end of the first connecting mixed liquid hole is communicated with the first waste liquid pool, the second connecting mixed liquid hole is staggered with any mixed flow channel or waste liquid pool, one end of the second connecting mixed liquid hole is communicated with the end of the first mixed flow channel, and the other end of the second connecting mixed liquid hole is communicated with the second mixed flow channel.

4. The microfluidic device for the detection of food additives according to claim 1, characterized in that: The third mobile valve comprises a third mobile transmission part outside the first lower chip body, a third mobile part is fixed on the third mobile transmission part and inserted into the third mobile channel, a first connecting waste liquid hole and a second connecting waste liquid hole are opened on the third mobile part, when the two ends of the first connecting waste liquid hole are respectively communicated with the first mixed flow channel and the first waste liquid pool, the two ends of the second connecting waste liquid hole are respectively staggered with the second mixed flow channel and the second waste liquid pool; when the two ends of the second connecting waste liquid hole are respectively communicated with the second mixed flow channel and the second waste liquid pool, the two ends of the first connecting waste liquid hole are respectively staggered with the second mixed flow channel and the second waste liquid pool.

5. The microfluidic device for the detection of food additives according to claim 3, characterized in that: The second lower chip body between the liquid storage pool and the third mixed flow channel is provided with a fourth mobile channel, and the second microfluidic chip further comprises a fourth mobile valve, the fourth mobile valve comprises a fourth mobile transmission part outside the second lower chip body, a fourth insertion hole is opened on the fourth mobile transmission part, a fourth mobile part is fixed on the fourth mobile transmission part and inserted into the fourth mobile channel, two first liquid passing holes and a second liquid passing hole are opened on the fourth mobile part, one end of the two first liquid passing holes is respectively communicated with the antigen storage pool and the first antibody storage pool, the other end of the first liquid passing hole is communicated with one end of the third mixed flow channel, and the second liquid passing hole is staggered with any other storage pool; when one end of the second liquid passing hole is communicated with the cleaning storage pool or the second antibody storage pool or the luminescent substrate storage pool, the other end of the second liquid passing hole is communicated with one end of the third mixed flow channel, and the two first liquid passing holes are staggered with any other storage pool.

6. The microfluidic device for the detection of food additives according to claim 5, characterized in that: The utility model also includes a detection shell, fixedly connected with a fixed frame in the detection shell, connected with a movable seat capable of moving horizontally on the fixed frame, equipped with a first injection assembly for injecting solution into the first micro fluidic chip and detecting food additives to be detected and a second injection assembly for injecting solution into each liquid storage pool in the second micro fluidic chip and detecting food additives to be detected on the movable seat, the first injection assembly includes a first connecting support connected on the movable seat, connected with a plurality of first injection supports on the first connecting support, fixedly connected with a plurality of first lifting motors on the first injection support, the first injection support below the first lifting motor is slidably connected with a first injection connecting block, the first injection connecting block is connected with a first injection liquid pipe, the first injection support is rotatably connected with a first injection screw rod corresponding to the first lifting motor, the first injection connecting block is screwedly connected on the corresponding first injection screw rod, and the first injection liquid pipe can inject solution into the corresponding first liquid storage pool; the second injection assembly includes a second connecting support connected on the movable seat, connected with a plurality of second injection supports on the second connecting support, fixedly connected with a plurality of second lifting motors on the second injection support, slidably connected with a second injection connecting block on the second injection support below the second lifting motor, connected with a second injection liquid pipe on the second injection connecting block, rotatably connected with a second injection screw rod corresponding to the second lifting motor on the second injection support, and the second injection connecting block is screwedly connected on the corresponding second injection screw rod, and the second injection liquid pipe can inject solution into the corresponding second liquid storage pool.

7. The microfluidic device for the detection of food additives according to claim 6, characterized in that: The utility model also includes two groups of clamping assemblies capable of moving horizontally and used for clamping the first micro fluidic chip and the second micro fluidic chip respectively, the clamping assembly includes a clamping base plate fixed in the detection shell, the clamping base plate is connected with a clamping support seat capable of sliding left and right on the upper side, the clamping base plate is fixedly connected with a clamping moving motor, the clamping base plate is rotatably connected with a clamping moving screw rod, the clamping moving screw rod is connected with the clamping moving motor, the clamping support seat is screwedly connected on the clamping moving screw rod, the clamping support seat is fixedly connected with a clamping support plate on the upper side, the clamping support plate is fixedly connected with a clamping support part at the front and rear ends respectively, a clamping motor is fixedly connected on the outer side of one clamping support part, the clamping motor is connected with a clamping screw rod rotatably connected between the two clamping support parts, a clamping moving block is screwedly connected on the clamping screw rod, a connecting part is fixedly connected on the upper side of one end of the clamping moving block extending out of the clamping support plate, the connecting part is fixedly connected with a movable clamping plate sliding along the upper side of the clamping support plate, the clamping support plate is fixedly connected with a fixed clamping plate oppositely arranged with the movable clamping plate, a first detection camera is fixedly connected on the fixed frame at one end of the first injection assembly in the left and right directions, a second detection camera is fixedly connected on the fixed frame at one end of the second injection assembly in the left and right directions, the first micro fluidic chip can be moved to below the first detection camera under the drive of the corresponding clamping seat, and the second micro fluidic chip can be moved to below the second detection camera under the drive of the corresponding clamping seat.

8. The microfluidic device for the detection of food additives according to claim 6, characterized in that: The first mobile transmission part is provided with a first insertion hole, the second mobile transmission part is provided with a second insertion hole, and the third mobile transmission part is provided with a third insertion hole. The first injection assembly further comprises a first upper connecting plate fixedly connected to one end of the first connecting support in the front-rear direction. A plurality of first guide rods are arranged on the first upper connecting plate. A first lower connecting plate is fixedly connected to the lower side of the first guide rod. A first transmission motor is fixedly connected to the upper side of the first upper connecting plate. A first transmission screw rod is rotatably connected between the first upper connecting plate and the first lower connecting plate. A first guide seat sliding along the first guide rod is threadedly connected to the first transmission screw rod. A first mobile screw rod, a second mobile screw rod, and a third mobile screw rod are rotatably connected to the first guide seat. A first mobile seat slidingly connected to the first guide seat is threadedly connected to the first mobile screw rod. A first mobile rod capable of being inserted into the first insertion hole is fixedly connected to the lower side of the first mobile seat. A second mobile seat slidingly connected to the second guide seat is threadedly connected to the second mobile screw rod. A second mobile rod capable of being inserted into the second insertion hole is fixedly connected to the lower side of the second mobile seat. A third mobile seat slidingly connected to the third guide seat is threadedly connected to the third mobile screw rod. A third mobile rod capable of being inserted into the third insertion hole is fixedly connected to the lower side of the third mobile seat.

9. The microfluidic device for the detection of food additives according to claim 6, characterized in that: The fourth mobile transmission part is provided with a fourth insertion hole. The second injection assembly further comprises a second upper connecting plate fixedly connected to one end of the second connecting support in the front-rear direction. A plurality of second guide rods are arranged on the second upper connecting plate. A second lower connecting plate is fixedly connected to the lower side of the second guide rod. A second transmission motor is fixedly connected to the upper side of the second upper connecting plate. A second transmission screw rod is rotatably connected between the second upper connecting plate and the second lower connecting plate. A second guide seat sliding along the second guide rod is threadedly connected to the second transmission screw rod. A fourth mobile screw rod is rotatably connected to the second guide seat. A fourth mobile seat slidingly connected to the second guide seat is threadedly connected to the fourth mobile screw rod. A fourth mobile rod capable of being inserted into the fourth insertion hole is fixedly connected to the lower side of the fourth mobile seat.

Images