High performance liquid chromatograph

Abstract

The invention relates to the technical field of high performance liquid chromatograph and temperature control thereof, in particular to a high performance liquid chromatograph and a temperature control method. The device comprises a temperature control conveying belt with a tray, and precise temperature control and ordered switching of reagent bottles are realized by arranging a residual liquid station, a heating station and a preparation station and cooperating with a baffle and a heating mechanism; the liquid supply module comprises a liquid supply main pipe, a residual liquid branch pipe and a heating branch pipe, and is combined with a transfer bottle, a negative pressure pump and a temperature control module to ensure stable liquid supply and maintain a proper temperature; and meanwhile, the coil-shaped heating branch pipe, the telescopic mechanism, the heating cavity and other assemblies are optimally designed, so that the heating efficiency and the operation convenience are improved. According to the invention, the effects of improving the reagent temperature control precision and continuity in the liquid chromatographic analysis process, simplifying the process and reducing the heat loss are achieved.

Description

Technical Field

[0001] This application relates to the field of detection equipment, and particularly to a high performance liquid chromatograph. Background Art

[0002] High performance liquid chromatography is one of the indispensable important tools in modern analytical chemistry, and is widely used in many fields such as pharmaceutical research, environmental monitoring, and food safety. This technology pumps a single solvent or a mixed solvent with different polarities into a chromatographic column filled with a stationary phase through a high-pressure liquid delivery system to achieve effective separation and accurate detection of the components of the sample to be measured. This process not only improves the accuracy and reliability of the analysis results, but also greatly shortens the experimental time and improves the efficiency of scientific research work.

[0003] The existing Chinese patent with the authorized announcement number of CN222050105U discloses a high performance liquid chromatograph, which includes a temperature control box. A sample box is fixedly connected to the top of the temperature control box. A tray rack is slidably connected to the inside of the sample box through a chute. A number of reagent bottle holes are provided on the tray rack. A heating mechanism is arranged below the reagent bottle holes. The heating mechanism includes a conductive base. Conductive contacts are arranged on both sides of the upper surface of the conductive base. Guide rings are fixedly connected to the four sides of the top of the conductive base. Guide grooves are provided on both sides of the guide ring, which enables the heating function to be automatically started after the reagent bottle is placed in the designated position.

[0004] The above related technology has the following defects: It can only replace a new reagent bottle when the reagent in a certain type of reagent bottle is used up, and the process of waiting for the new reagent bottle to heat up is relatively long, reducing the overall use efficiency of the equipment. Summary of the Invention

[0005] In order to improve the overall efficiency of the equipment during the process of replacing the reagent bottle, this application provides a high performance liquid chromatograph.

[0006] A high performance liquid chromatograph includes a temperature control conveyor belt. A number of trays for placing reagent bottles are evenly distributed on the temperature control conveyor belt. There are at least three workstations above the temperature control conveyor belt, which are a residual liquid workstation, a heating workstation, and a preparation workstation in sequence. A baffle is arranged on the side of the temperature control conveyor belt. Heating mechanisms are arranged on both sides of the baffle at the heating workstation.

[0007] The liquid chromatograph further includes a liquid supply module. The liquid supply module includes a liquid supply main pipe and a residual liquid branch pipe and a heating branch pipe both connected to the liquid supply main pipe. The residual liquid branch pipe extends into the reagent bottle placed at the residual liquid workstation, and the heating branch pipe extends into the reagent bottle placed at the heating workstation.

[0008] By adopting the above technical solution, the trays on the temperature-controlled conveyor belt are used to place reagent bottles, which are divided into a residual liquid station, a heating station, and a preparation station, and cooperate with the residual liquid branch pipe and the heating branch pipe in the liquid supply module to extract the reagents in the reagent bottles at corresponding positions respectively. In this way, when replacing the reagent bottles, the residual heat of the previous reagent bottle can be utilized to continuously supply the liquid while heating the new reagent bottle, reducing the waiting time, improving the overall utilization efficiency of the equipment, and realizing a more continuous liquid supply process.

[0009] The specific operation method for replacing the reagent bottles is as follows: before the reagent in the reagent bottle at the heating station is used up, first withdraw the liquid supply module, and then use the temperature-controlled conveyor belt to move the reagent bottle still containing reagent to the residual liquid station, move the reagent bottle at the preparation station to the heating station, and then re-insert the liquid supply module into the corresponding reagent bottle. Thereafter, the residual liquid branch pipe extracts the remaining relatively warm reagent, and during this process, the new reagent bottle can be heated synchronously under the action of the heating mechanism. When the reagent in the reagent bottle at the residual liquid station is completely pumped out, the reagent in the reagent bottle at the heating station has also been heated to the required temperature, and then the heating branch pipe can extract the reagent in the new reagent bottle. Therefore, the replacement process of the reagent bottles in this application has a higher degree of coordination. The time for continuously supplying the liquid by utilizing the residual heat of the previous reagent bottle is used to raise the temperature of the latter reagent bottle, so that a new reagent bottle preheated to the required temperature can be obtained before the previous reagent bottle is completely used up, thus enabling the entire equipment not to wait for a long time during the process of replacing the reagent bottles, realizing a relatively continuous liquid supply, and further improving the overall utilization efficiency.

[0010] In addition, the power of the heating mechanism can also be increased during the process of replacing the reagent bottles to appropriately raise the temperature of the previous reagent bottle. After moving the workstations, the residual liquid branch pipe is used to extract the relatively warm residual reagent, the heating branch pipe is used to extract the reagent in the new reagent bottle at a relatively low temperature, and they are mixed in the liquid supply branch pipe to directly obtain the reagent at the required temperature by proportioning. The residual liquid branch pipe and the heating branch pipe can be respectively provided with independent flow regulating valves to respectively control the flow rate and opening and closing of their own.

[0011] Preferably, the liquid supply module further includes a transfer bottle, the top of the transfer bottle is provided with a negative pressure pump, the end of the liquid supply main pipe extends into the interior of the transfer bottle and the end is above the liquid level, and the transfer bottle is provided with a temperature control module.

[0012] By adopting the above technical solution, the setting of the transfer bottle can temporarily store the reagents transported from the residual liquid branch pipe and the heating branch pipe, and the negative pressure pump is used to effectively suck the reagents. The temperature control module is arranged in the transfer bottle, which can accurately control the temperature of the mixed reagents to ensure that the reagents maintain the required temperature state during the supply process, thereby improving the stability and reliability of the entire system.

[0013] Preferably, the transfer bottle is further connected with a transfer pipe. The end of the transfer pipe extends into the interior of the transfer bottle and is located below the liquid level. The transfer pipe is connected with a transfer pump, and a check valve is arranged inside the transfer pipe.

[0014] By adopting the above technical solution, the end of the transfer pipe extends into the interior of the transfer bottle and is located below the liquid level, ensuring that the transfer pump can effectively extract the reagent in the transfer bottle and prevent air from entering to affect the liquid supply stability. The check valve arranged inside the transfer pipe effectively avoids the backflow of the reagent, ensuring the normal operation of the system and the purity of the reagent.

[0015] Preferably, the section of the heating branch pipe extending into the reagent bottle is in a coiled shape and is arranged in contact with the inner side wall of the reagent bottle.

[0016] By adopting the above technical solution, the section of the heating branch pipe extending into the reagent bottle is designed to be in a coiled shape and is arranged in contact with the inner side wall of the reagent bottle. This structure enables the heating branch pipe to be in contact with the heat source for a longer time during the process of extracting the reagent, thereby accelerating the heating process of the reagent at the heating station. Specifically, when a new reagent bottle is conveyed to the heating station, although the initial heating is uneven, due to the coiled design, the reagent can gradually absorb heat while being transported along the pipeline, ensuring that it reaches the required temperature before reaching the outlet. This not only improves the efficiency when the new reagent bottle is officially put into use, but also facilitates the synchronous liquid supply of the reagent bottles between the residual liquid station and the heating station, promoting the mixed reagent to quickly reach the required temperature requirements.

[0017] Preferably, the baffle is provided with a telescopic mechanism, and the heating mechanism is connected to the telescopic mechanism.

[0018] By adopting the above technical solution, the setting of the telescopic mechanism can flexibly adjust the distance between the heating mechanism and the reagent bottle. This design not only helps to accurately control the heating speed and heating effect, but also can adapt to reagent bottles of different specifications, improving the versatility and flexibility of the equipment. Specifically, this technical means allows users to dynamically adjust the heating conditions according to actual needs, thereby optimizing the heating process, reducing energy waste and enhancing the overall working efficiency.

[0019] Preferably, both the telescopic mechanism and the heating mechanism are provided in a pair. The heating mechanism includes a heating plate. A heating groove is arranged on the side of the heating plate facing the heating station. When the two heating plates are attached, the two heating grooves enclose a heating chamber for placing the reagent bottle.

[0020] By adopting the above technical solution, a pair of telescopic mechanisms and heating mechanisms are provided, and a heating groove is provided on the side of the heating plate facing the heating station. When the two heating plates are attached to each other, a heating chamber for placing the reagent bottle is formed. This design can more precisely control the heating speed and heating effect, because the distance between the heating plate and the reagent bottle can be changed by adjusting the telescopic mechanism, thereby flexibly adjusting the heating intensity. At the same time, the design of the heating chamber makes the heat more concentrated, improves the heating efficiency, ensures that the reagent in the reagent bottle can reach the required temperature in a shorter time, and further improves the overall use efficiency of the equipment.

[0021] Preferably, the baffle is provided with a lifting mechanism for driving the liquid supply module, and the main liquid supply pipe is a flexible hose.

[0022] By adopting the above technical solution, the baffle is provided with a lifting mechanism for driving the liquid supply module, and the main liquid supply pipe is a flexible hose. This design can uniformly realize the lifting of the residual liquid branch pipe and the heating branch pipe, reduce the degree of manual intervention, improve the degree of automation, and accelerate the overall replacement efficiency of the reagent bottle. At the same time, the design of the flexible hose makes the main liquid supply pipe move more flexibly with the lifting mechanism, and will not cause inconvenience in operation or damage to the equipment due to hard pipes.

[0023] Preferably, an inner support spring is arranged inside the main liquid supply pipe.

[0024] By adopting the above technical solution, the inner support spring can maintain the bending freedom of the main liquid supply pipe, facilitate the lifting mechanism to drive the liquid supply module to lift, improve the degree of automation and efficiency in the process of replacing the reagent bottle. At the same time, the inner support spring causes the reagent to rotate to a certain extent when flowing inside the main liquid supply pipe, forming a turbulent flow effect, which helps the two reagents at different temperatures to mix better during the transportation process, promotes heat exchange, and ensures that the temperature of the mixed reagent is more uniform.

[0025] Preferably, a flow regulating valve is respectively arranged on the residual liquid branch pipe and the heating branch pipe.

[0026] By adopting the above technical solution, the flow rate and opening and closing of the residual liquid branch pipe and the heating branch pipe can be independently controlled. This design enables the working states of the two branch pipes to be flexibly adjusted according to actual needs during the process of replacing the reagent bottle, ensuring that the reagent supply is more accurate and stable, and improving the degree of automation and use efficiency of the equipment. Especially when mixing reagents at different temperatures, the proportion of heat exchange can be better and precisely controlled by adjusting the flow rate, so that the temperature of the finally output reagent is more uniform.

[0027] A temperature control method based on a high performance liquid chromatography instrument: Before the reagent in the reagent bottle at the heating station is used up, first withdraw the liquid supply module and temporarily suspend the supply of the reagent; then use the temperature-controlled conveyor belt to move the reagent bottle still having the reagent to the residual liquid station, and move the reagent bottle at the preparation station to the heating station; then re-insert the liquid supply module into the corresponding reagent bottle, and then extract the remaining relatively high-temperature reagent from the residual liquid branch pipe; before the reagent in the reagent bottle at the residual liquid station is completely drained, use the heating mechanism to synchronously heat the reagent bottle at the heating station until the reagent in the reagent bottle at the heating station is also raised to the required temperature, and then the reagent in the reagent bottle at the heating station can be extracted from the heating branch pipe.

[0028] By adopting the above technical solution, the continuity and use efficiency of the high performance liquid chromatography instrument during the process of replacing the reagent bottle can be significantly improved. Specifically: Before the reagent in the reagent bottle at the heating station is used up, first withdraw the liquid supply module and temporarily suspend the supply of the reagent, avoiding a long interruption caused by insufficient reagent. Use the temperature-controlled conveyor belt to move the reagent bottle still having the reagent to the residual liquid station, and move the reagent bottle at the preparation station to the heating station, realizing an effective switching of the positions of the reagent bottles and reducing manual intervention. After re-inserting the liquid supply module into the corresponding reagent bottle again, extract the remaining relatively high-temperature reagent from the residual liquid branch pipe, making full use of the remaining temperature of the previous reagent bottle and improving the resource utilization rate. Before the reagent in the reagent bottle at the residual liquid station is completely drained, use the heating mechanism to synchronously heat the reagent bottle at the heating station to ensure that the new reagent bottle can reach the required temperature in a short time, so as to achieve rapid commissioning. Finally, extract the reagent in the reagent bottle at the heating station from the heating branch pipe, ensuring a seamless connection between the new and old reagent bottles and enhancing the operating efficiency and stability of the overall equipment.

[0029] In summary, the present application includes at least one of the following beneficial technical effects:

[0030] 1. By setting a temperature-controlled conveyor belt and multiple stations (residual liquid station, heating station and preparation station), continuous liquid supply can be achieved during the process of replacing the reagent bottle, avoiding downtime caused by waiting for the new reagent bottle to heat up, and significantly enhancing the overall use efficiency of the equipment;

[0031] 2. The residual liquid branch pipe and the heating branch pipe extract reagents from different stations respectively. By making use of the remaining temperature of the previous reagent bottle and the synchronous heating of the next reagent bottle, a seamless connection of reagent supply is realized, solving the interruption problem existing in the traditional equipment during the reagent bottle switching;

[0032] 3. A section of the heating branch pipe extending into the reagent bottle is designed as a coiled pipe and fits the inner wall, enabling the reagent to fully contact the heat source during the extraction process, accelerating the heating rate, and further improving the efficiency of putting the new reagent bottle into use. Description of the Drawings

[0033] Figure 1 is a schematic structural diagram of an embodiment of the present application;

[0034] Figure 2 is a schematic structural diagram for showing the connection relationship between the heating branch pipe and the reagent bottle in an embodiment of the present application;

[0035] Figure 3 is a schematic structural diagram for showing the connection relationship between the inner support spring and the liquid supply main pipe in an embodiment of the present application;

[0036] Figure 4 is a schematic structural diagram for showing the connection relationship between the heating plate and the telescopic mechanism in an embodiment of the present application;

[0037] Figure 5 is a schematic structural diagram for showing the connection relationship between the flow regulating valve and the residual liquid branch pipe in an embodiment of the present application;

[0038] Figure 6 is a schematic structural diagram for showing the connection relationship between the liquid supply main pipe and the transfer bottle in an embodiment of the present application.

[0039] In the figure:

[0040] 1. Temperature control conveyor belt; 11. Residual liquid station; 12. Heating station; 13. Preparation station; 14. Baffle; 15. Reagent bottle;

[0041] 2. Liquid supply main pipe; 21. Residual liquid branch pipe; 22. Heating branch pipe; 23. Lifting mechanism; 24. Inner support spring; 25. Flow regulating valve;

[0042] 3. Heating plate; 30. Heating groove; 31. Telescopic mechanism;

[0043] 4. Transfer bottle; 41. Negative pressure pump; 42. Temperature control module; 43. Transfer pipe; 44. Transfer pump; 45. Check valve. Detailed Embodiments

[0044] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the drawings. The described embodiments are only possible technical implementations of the present invention, not all possible implementations. Those skilled in the art can fully combine the embodiments of the present invention to obtain other embodiments without creative labor, and these embodiments are also within the protection scope of the present invention.

[0045] The inventors of the present application have found that there are obvious deficiencies in the existing high-performance liquid chromatography instruments when dealing with the replacement of reagent bottles in multiple batches. In particular, when the reagent in a certain reagent bottle is used up, it is necessary to wait for the new reagent bottle to be preheated to the required temperature, during which the equipment cannot work properly, resulting in low overall use efficiency. For this reason, the present application mainly adopts the following solutions to achieve efficient replacement of reagent bottles without interrupting the experimental process, significantly improving the overall working efficiency. The following is a further detailed description of the present application.

[0046] Embodiment

[0047] Refer to Figure 1 , a high-performance liquid chromatography instrument provided by an embodiment of the present application includes a temperature-controlled conveyor belt 1. The temperature-controlled conveyor belt 1 is a chain-type conveyor belt and is supported by a frame in cooperation with a baffle 14 for installation. There are at least three workstations above the temperature-controlled conveyor belt 1, which are in sequence a residual liquid workstation 11, a heating workstation 12, and a preparation workstation 13. In the actual working state, reagent bottles 15 are placed at the residual liquid workstation 11, the heating workstation 12, and the preparation workstation 13. The reagent bottle 15 at the preparation workstation 13 is in a new unused state, the reagent bottle 15 at the processing workstation is in a relatively full state, and the reagent bottle 15 at the residual liquid workstation 11 is in a relatively less state. The conveying direction of the temperature-controlled conveyor belt 1 is from the preparation workstation 13 towards the residual liquid workstation 11 for conveying.

[0048] Refer to Figure 2 , the liquid chromatography instrument further includes a liquid supply module. The liquid supply module includes a liquid supply main pipe 2 and a residual liquid branch pipe 21 and a heating branch pipe 22 both connected to the liquid supply main pipe 2. The liquid supply main pipe 2 is a flexible pipe. The bottom of the residual liquid branch pipe 21 extends into the reagent bottle 15 placed at the residual liquid workstation 11, and the bottom of the heating branch pipe 22 extends into the reagent bottle 15 placed at the heating workstation 12.

[0049] Refer to Figure 1 and Figure 2 , the baffle 14 is further provided with a lifting mechanism 23 for driving the liquid supply module. In this embodiment, the lifting mechanism 23 adopts a cylinder and the driving end of the cylinder is fixedly arranged with the residual liquid branch pipe 21 and the heating branch pipe 22 as a whole. Since both the residual liquid branch pipe 21 and the heating branch pipe 22 are rigid pipes, the lifting mechanism 23 can uniformly lift the residual liquid branch pipe 21 and the heating branch pipe 22. During this process, the liquid supply main pipe 2 bends and deforms passively by virtue of its own flexibility, so as to reduce the degree of manual intervention, improve the degree of automation, and further improve the overall replacement efficiency of the reagent bottle 15.

[0050] The operation method of replacing the reagent bottle 15 is as follows. Before the reagent in the reagent bottle 15 at the heating station 12 is used up, the liquid supply module is first withdrawn. Then, the reagent bottle 15 still containing reagent is moved to the residual liquid station 11 by using the temperature-controlled conveyor belt 1. The reagent bottle 15 at the preparation station 13 is moved to the heating station 12. Then, the liquid supply module is inserted back into the corresponding reagent bottle 15. Thereafter, the remaining relatively hot reagent is extracted by the residual liquid branch pipe 21. During this process, the new reagent bottle 15 can be heated synchronously under the action of the heating mechanism. When the reagent in the reagent bottle 15 at the residual liquid station 11 is completely extracted, the reagent in the reagent bottle 15 at the heating station 12 has also been heated to the required temperature. Then, the reagent in the new reagent bottle 15 can be extracted by the heating branch pipe 22. Therefore, the replacement process of the reagent bottle 15 in the present application has a higher degree of coordination. By utilizing the remaining temperature of the previous reagent bottle 15 for the time of continuous liquid supply, the temperature of the subsequent reagent bottle 15 is raised. In this way, a new reagent bottle 15 that has been preheated to the required temperature can be obtained before the previous reagent bottle 15 is completely used up, so that the overall equipment does not need to wait for a long time during the process of replacing the reagent bottle 15, realizing relatively continuous liquid supply, and thus improving the overall use efficiency. In addition, the power of the heating mechanism can also be increased during the process of replacing the reagent bottle 15 to appropriately raise the temperature of the previous reagent bottle 15. After moving the station, the relatively hot residual reagent is extracted by the residual liquid branch pipe 21, and the reagent in the new reagent bottle 15 at a lower temperature is extracted by the heating branch pipe 22 and mixed in the liquid supply branch pipe to directly obtain the reagent at the required temperature by proportioning.

[0051] Referring to Figure 3 , an inner support spring 24 is arranged inside the liquid supply main pipe 2. On the one hand, it maintains the bending freedom of the liquid supply main pipe 2, facilitating the lifting mechanism 23 to drive the liquid supply module to lift and lower. On the other hand, the inner support spring 24 can cause the reagent to rotate to a certain extent during the internal flow process, and realizes further mixing during the conveying process by using turbulent flow. When the reagent supply mode is that the residual liquid branch pipe 21 and the heating branch pipe 22 jointly pump and send, it can effectively promote the fusion of two reagents at different temperatures, better conduct heat exchange, and promote the temperature uniformity of the fused reagent.

[0052] Referring to Figure 4 , heating mechanisms are arranged on both sides of the heating station 12, and the heating mechanisms are connected to the baffle 14 through the telescopic mechanism 31 for installation. The telescopic mechanism 31 selects a cylinder in this embodiment. By adjusting the length of the telescopic rod of the telescopic mechanism 31 extending out, the distance between the heating mechanism and the reagent bottle 15 can be accurately controlled, thereby assisting in adjusting the heating speed and heating effect. In addition, when the temperature-controlled conveyor belt 1 needs to convey the reagent bottle 15, the heating mechanism can also be temporarily moved away from the reagent bottle 15 at the heating station 12 by using the telescopic mechanism 31 to facilitate the automatic conveyance of the reagent bottle 15.

[0053] Referring toFigure 5 , the heating mechanism is a heating plate 3. On the side of the heating plate 3 facing the heating station 12, there is a heating groove 30. When the two heating plates 3 are fitted together, the two heating grooves 30 can enclose a heating chamber for placing the reagent bottle 15, and there is a pore at the top only for the heating branch pipe 22 to pass through, so as to better realize the temperature rise of the reagent bottle 15 at the heating station 12. The actual heating method of the heating plate 3 can select various types such as resistance wires and ceramic heating elements, and the power size is determined according to actual needs.

[0054] Refer to Figure 5 , the residual liquid branch pipe 21 and the heating branch pipe 22 are respectively configured with independent flow regulating valves 25. The flow regulating valve 25 can select the type of manual or electric adjustment. Taking the manual flow regulating valve 25 as an example, it generally includes three main parts: a handwheel, a valve core and a sealing ring. The handwheel is responsible for manually adjusting the opening degree, and the material is recommended to be made of aluminum alloy to reduce the weight; the valve core determines the flow area, and it is recommended to be made of stainless steel to ensure durability; the sealing ring prevents leakage, and fluororubber products are preferably selected to adapt to a wide temperature range. The two are used in combination to accurately control the flow rate in their respective channels and meet the mixing operation requirements in different application scenarios.

[0055] Refer to Figure 5 , in addition, the section of the heating branch pipe 22 extending into the reagent bottle 15 is in a coiled shape and is arranged in contact with the inner side wall of the reagent bottle 15. When a new reagent bottle 15 is just transferred to the heating station 12, the heating mechanism gradually heats the reagent bottle 15. However, it still takes a long time to completely heat the reagent in the reagent bottle 15 evenly. Therefore, in this application, the section extending into the reagent bottle 15 is set in a coiled shape in contact with the inner wall of the reagent bottle 15, which is closer to the heat source. Therefore, during the process of the reagent being pumped and sent along the coiled heating branch pipe 22, it can be in contact with the heat source and be transported for a long time, and the temperature can be gradually increased during the transportation process to heat the reagent to the required temperature, so as to further improve the efficiency of the new reagent bottle 15 being officially put into use, and it is more convenient to supply liquid to the reagent bottles 15 at the residual liquid station 11 and the heating station 12 at the same time, so that the mixed reagent can reach the required temperature requirements faster.

[0056] Refer to Figure 1 and Figure 6, In addition, the liquid supply module further includes a transfer bottle 4. A negative pressure pump 41 is provided at the top of the transfer bottle 4 to maintain a negative pressure state inside the transfer bottle 4. The top end of the main liquid supply pipe 2 extends into the transfer bottle 4 and the end is above the liquid level, so that the reagent can be continuously sucked into the transfer bottle 4 by negative pressure. A temperature control module 42 is provided at the bottom of the transfer bottle 4 to facilitate temperature control of the reagent (especially the mixed reagent) inside the transfer bottle 4 and assist in adjusting the reagent temperature. The transfer bottle 4 is also connected with a transfer pipe 43. One end of the transfer pipe 43 extends into the transfer bottle 4 and the end is below the liquid level. The other end of the transfer pipe 43 extends out of the transfer bottle 4 and is connected with a transfer pump 44 to pump out the reagent with the finally adjusted temperature inside the transfer bottle 4. In addition, a check valve 45 is provided on a section of the transfer pipe 43 located inside the transfer bottle 4 to reduce the influence of the negative pressure inside the transfer bottle 4 on the transfer pipe 43. The design of the transfer bottle 4 not only effectively reduces pressure fluctuations and improves the stability of the system, but also reduces the temperature loss or other instabilities of the reagent during pumping and transportation through the secondary temperature control method, thereby further improving the overall performance and reliability of the system.

[0057] The implementation principle of this embodiment is as follows: The entire system operation mechanism is constructed around the three major principles of "advance preparation - alternating operation - seamless connection". When a reagent in service is about to run out, a fresh member in the standby queue is immediately started for preheating. By arranging the sequence of each operation link with a precisely calculated time difference, it is ensured that there is always a sufficient amount of solution at the appropriate temperature available for use at any time. Compared with the traditional method that simply relies on the rotation of a single container for supply, it is obviously more flexible and intelligent, greatly reducing the length of the idle interval period, and thus comprehensively improving the performance index of the overall operation.

[0058] Especially during the actual operation process, before the reagent bottle 15 is displaced, the power of the heating mechanism can be appropriately increased to raise the initial temperature of the reagent in the reagent bottle 15 at the subsequent residual liquid station 11, further shortening the preheating time, accelerating the bottle replacement speed, and improving the overall working efficiency. In addition, through the design of the transfer bottle 4, the pressure fluctuation phenomenon caused by frequent start-stop operations is reduced, further optimizing the stability and reliability of the system. In short, through a series of innovative designs, this application significantly improves the working efficiency and stability of the high-performance liquid chromatograph during the replacement of multiple batches of reagent bottles 15, and has a wide range of application prospects.

[0059] In addition, this embodiment also discloses a temperature control method based on a high performance liquid chromatograph: before the reagent in the reagent bottle 15 at the heating station 12 is used up, the liquid supply module is first withdrawn and the supply of the reagent is temporarily suspended; then the reagent bottle 15 still containing the reagent is moved to the residual liquid station 11 by using the temperature control conveyor belt 1, and the reagent bottle 15 at the preparation station 13 is moved to the heating station 12; then the liquid supply module is inserted back into the corresponding reagent bottle 15, and then the remaining reagent with a relatively high temperature is extracted by the residual liquid branch pipe 21; before the reagent in the reagent bottle 15 at the residual liquid station 11 is completely emptied, the reagent bottle 15 at the heating station 12 is heated synchronously by using the heating mechanism until the reagent in the reagent bottle 15 at the heating station 12 is also raised to the required temperature, and then the reagent in the reagent bottle 15 at the heating station 12 can be extracted by the heating branch pipe 22.

[0060] The implementation principle of this embodiment is as follows: the replacement process of the reagent bottle 15 in this application has a higher degree of coordination. The time for continuous liquid supply by using the residual heat of the previous reagent bottle 15 is used to raise the temperature of the next reagent bottle 15, so that a new reagent bottle 15 preheated to the required temperature can be obtained before the previous reagent bottle 15 is completely used up, thereby enabling the overall equipment to not need to wait for a long time during the process of replacing the reagent bottle 15, realizing relatively continuous liquid supply, and further improving the overall use efficiency. In the actual operation process, before the reagent bottle 15 is displaced, the power of the heating mechanism can also be appropriately increased to raise the initial temperature of the reagent in the reagent bottle 15 at the subsequent residual liquid station 11.

[0061] The above are all the preferred embodiments of this application, and the protection scope of this application is not limited accordingly. Therefore, all equivalent changes made according to the structure, shape, and principle of this application should be covered within the protection scope of this application.

Claims

1. A high performance liquid chromatograph, characterized in that: The invention comprises a temperature-controlled conveyor belt (1), on which a plurality of trays for placing reagent bottles (15) are evenly distributed, and above the temperature-controlled conveyor belt (1) there are at least three workstations, which are a residual liquid workstation (11), a heating workstation (12) and a preparation workstation (13) in sequence, and baffles (14) are arranged on the sides of the temperature-controlled conveyor belt (1), and heating mechanisms are arranged on both sides of the baffles (14) located at the heating workstation (12); The liquid chromatograph further comprises a liquid supply module, the liquid supply module comprising a liquid supply main pipe (2), a residual liquid branch pipe (21) and a heating branch pipe (22) both connected to the liquid supply main pipe (2), the residual liquid branch pipe (21) extending into a reagent bottle (15) placed at the residual liquid station (11), and the heating branch pipe (22) extending into a reagent bottle (15) placed at the heating station (12).

2. A high performance liquid chromatograph according to claim 1, characterized in that: The liquid supply module also includes a transfer bottle (4), a negative pressure pump (41) is arranged on the top of the transfer bottle (4), the end of the liquid supply main pipe (2) extends into the interior of the transfer bottle (4) and the end is located above the liquid surface, and the transfer bottle (4) is provided with a temperature control module (42).

3. A high performance liquid chromatograph according to claim 2, characterized in that: The transfer bottle (4) is also connected to a transfer pipe (43), the end of which extends into the interior of the transfer bottle (4) and is located below the liquid surface. The transfer pipe (43) is connected to a transfer pump (44), and a check valve (45) is arranged inside the transfer pipe (43).

4. A high performance liquid chromatograph according to claim 1, characterized in that: The section of the heating branch pipe (22) extending into the interior of the reagent bottle (15) is in the shape of a coil and is arranged in close contact with the inner wall of the reagent bottle (15).

5. A high performance liquid chromatograph according to claim 4, characterized in that: The baffle (14) is provided with a telescopic mechanism (31), and the heating mechanism is connected to the telescopic mechanism (31).

6. A high performance liquid chromatograph according to claim 5, characterized in that: The telescopic mechanism (31) and the heating mechanism are both arranged as a pair, the heating mechanism comprising a heating plate (3), a heating groove (30) being arranged on the side of the heating plate (3) facing the heating station (12), and when the two heating plates (3) are attached to each other, the two heating grooves (30) enclose a heating chamber for placing a reagent bottle (15).

7. A high performance liquid chromatograph according to claim 1, characterized in that: The baffle (14) is provided with a lifting mechanism (23) for driving the liquid supply module, and the liquid supply main pipe (2) is a hose.

8. A high performance liquid chromatograph according to claim 7, characterized in that: An internal support spring (24) is arranged inside the liquid supply main pipe (2).

9. A high performance liquid chromatograph according to claim 1, characterized in that: The residual liquid branch pipe (21) and the heating branch pipe (22) are each provided with a flow regulating valve (25).

10. A temperature control method based on the high performance liquid chromatograph according to any one of claims 1 to 9, characterized in that: Before the reagent in the reagent bottle (15) at the heating station (12) is used up, the liquid supply module is first drawn out to temporarily suspend the supply of reagent; then the reagent bottle (15) still containing the reagent is moved to the residual liquid station (11) by using the temperature-controlled conveyor belt (1), and the reagent bottle (15) at the preparation station (13) is moved to the heating station (12); then the liquid supply module is re-inserted into the corresponding reagent bottle (15), and then the remaining reagent is extracted by the residual liquid branch pipe (21); before the reagent in the reagent bottle (15) at the residual liquid station (11) is completely extracted, the reagent bottle (15) at the heating station (12) is synchronously heated by using the heating mechanism until the reagent in the reagent bottle (15) at the heating station (12) is also raised to the required temperature, and then the reagent in the reagent bottle (15) at the heating station (12) can be extracted by the heating branch pipe (22).

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