Medicated diet formula, food, health-care food and medicine based on traditional Chinese medicine five-element kidney attributes and preparation method and application of medicated diet formula, food, health-care food and medicine

Abstract

The invention provides a medicated diet formula, food, health-care food and medicine based on traditional Chinese medicine five-element kidney attributes as well as a preparation method and application thereof, and belongs to the field of pharmaceutical compositions. The medicated diet formula is prepared from the following raw medicinal materials in parts by weight: 10-14 parts of rhizoma polygonati, 1-3 parts of cinnamon, 8-12 parts of raw Chinese yam, 8-12 parts of raw gordon euryale seed, 2-4 parts of fructus alpiniae oxyphyllae, 8-12 parts of medlar, 4-6 parts of mulberry, 4-6 parts of raspberry, 4-6 parts of lotus seed, 8-12 parts of Chinese date, 3-5 parts of dried ginger and 2-4 parts of lucid ganoderma. Based on the five-element characteristic of the five-element kidney attribute of traditional Chinese medicine, all the medicines supplement one another, tonify the kidney, strengthen essence, nourish yin and promote the secretion of saliva or body fluid, and the medicated diet composition can remarkably resist body immunosuppression caused by cyclophosphamide and is used for enhancing immunity. All the medicinal raw materials are medicinal and edible products, are safe and effective, and have a good application prospect.

Description

Technical Field

[0001] The present invention belongs to the field of pharmaceutical compositions, and particularly relates to a medicated diet formula, food, health food, drug based on the kidney attribute of the five elements in traditional Chinese medicine, and its preparation method and use. Background Art

[0002] Low immunity refers to the reduction of the defense level of the body's immune system, which is unable to effectively identify and eliminate pathogenic microorganisms and abnormal cells in the body, resulting in the body being easily invaded by infections and diseases. Patients with low immunity may show symptoms such as fatigue, general discomfort, fever, repeated infections, allergies, joint swelling and pain, etc. Common causes leading to low immunity include: aging, lack of essential nutrients, insufficient sleep, excessive stress, bad living habits, psychological factors (such as adverse emotions like mental tension, anxiety, depression, etc.), and disease factors (such as immunodeficiency diseases, autoimmune diseases, etc.).

[0003] Low immunity can be improved by means such as balanced diet, strengthening exercise, ensuring sufficient sleep, reducing stress, and drug treatment. As a common method, a balanced diet can include eating more foods rich in vitamins and high-quality proteins, such as lemons, oranges, pitayas, kiwis, lean meats, eggs, milk, etc.; insisting on aerobic exercises, such as brisk walking, yoga, swimming, etc., can enhance physical fitness and improve immunity; ensuring sufficient high-quality sleep every day helps the normal operation of the immune system; maintaining an optimistic attitude, reducing stress, and avoiding being in an anxious state for a long time. Drug treatment mainly involves using drugs such as pidotimod granules, Yupingfeng granules, and multivitamin tablets under the guidance of a doctor to improve physical fitness and enhance immunity.

[0004] Traditional Chinese medicine believes that low immunity is usually related to qi and blood deficiency and disharmony of zang-fu functions. Specific manifestations are as follows: weakness of the spleen and stomach. The spleen and stomach are the foundation of acquired constitution and the source of qi and blood generation. Weakness of the spleen and stomach will lead to qi and blood deficiency, affecting immunity; stagnation of liver qi: long-term emotional depression will lead to stagnation of liver qi, affecting qi and blood circulation, and then affecting immunity; insufficiency of kidney essence: the kidney stores essence and is responsible for growth, development, and reproduction. Insufficiency of kidney essence will lead to physical weakness and decreased immunity. Therefore, it is often necessary to regulate the spleen and stomach, improve the function of the spleen and stomach, and enhance the source of qi and blood generation; tonify the kidney and replenish essence, improve insufficiency of kidney essence, and enhance the body's disease resistance. Through the above methods, the condition of low immunity can be effectively improved, and the body's disease resistance can be enhanced. Medicated diet, as a treatment method combining traditional Chinese medicine and food, can effectively enhance the body's immune ability through reasonable collocation. Therefore, developing different forms of medicated diets can also provide more choices for people with low immunity. Summary of the Invention

[0005] The purpose of the present invention is to provide a medicated diet formula, food, health food, drug based on the kidney attribute of the five elements in traditional Chinese medicine, and its preparation method and use.

[0006] The present invention provides a medicinal diet formula, which is prepared from raw medicinal materials in the following weight ratios: 10-14 parts of polygonatum sibiricum, 1-3 parts of cinnamon, 8-12 parts of raw Chinese yam, 8-12 parts of raw euryale ferox, 2-4 parts of semen alpina galangae, 8-12 parts of wolfberry, 4-6 parts of mulberry, 4-6 parts of raspberry, 4-6 parts of lotus seed, 8-12 parts of Chinese date, 3-5 parts of dried ginger, and 2-4 parts of ganoderma lucidum.

[0007] Further, it is prepared from raw medicinal materials in the following weight ratios: 12 parts of polygonatum sibiricum, 2 parts of cinnamon, 10 parts of raw Chinese yam, 10 parts of raw euryale ferox, 3 parts of semen alpina galangae, 10 parts of wolfberry, 5 parts of mulberry, 5 parts of raspberry, 5 parts of lotus seed, 10 parts of Chinese date, 4 parts of dried ginger, and 3 parts of ganoderma lucidum.

[0008] Further, it is a food or pharmaceutical preparation prepared from the powder of the raw medicinal materials in the above weight ratios, or the water and / or organic solvent extract of the raw medicinal materials as the active ingredient, plus food or pharmaceutically acceptable excipients or auxiliary components.

[0009] The present invention also provides a method for preparing the above medicinal diet formula. The method includes the following steps: taking the raw medicinal materials in each weight ratio, directly pulverizing them, or adding water or an organic solvent for extraction, and then adding food or pharmaceutically acceptable excipients or auxiliary components to obtain a food or pharmaceutical preparation.

[0010] The present invention also provides the use of the above medicinal diet formula in the preparation of health foods helpful for enhancing immunity.

[0011] The present invention also provides the use of the above medicinal diet formula in the preparation of foods.

[0012] The present invention also provides the use of the above medicinal diet formula in the preparation of drugs for improving immunity.

[0013] The present invention also provides a food, which comprises the above medicinal diet formula.

[0014] The present invention also provides a health food helpful for enhancing immunity, which is prepared from the above medicinal diet formula as the active ingredient, plus excipients or auxiliary components acceptable for health foods.

[0015] The present invention also provides a drug for improving immunity, which is prepared from the above medicinal diet formula as the active ingredient, plus pharmaceutically acceptable excipients or auxiliary components.

[0016] The formula composition of the present invention is as follows: 12 g of polygonatum sibiricum, 2 g of cinnamon, 10 g of fresh Chinese yam, 10 g of euryale ferox, 3 g of semen alpiniae oxyphyllae, 10 g of wolfberry fruit, 5 g of mulberry, 5 g of raspberry, 5 g of lotus seed, 10 g of Chinese date, 4 g of dried ginger, and 3 g of ganoderma lucidum. In the formula, polygonatum sibiricum nourishes the kidney and replenishes essence, supplements qi and nourishes yin. The combination of fresh Chinese yam and wolfberry fruit can nourish the kidney, replenish essence and nourish yin. The combination of these three herbs can achieve the effect of tonifying the kidney, replenishing qi and nourishing yin, and they are the sovereign herbs of this formula. The combination of euryale ferox and lotus seed can achieve the effects of invigorating the spleen and stopping diarrhea, tonifying the kidney and arresting seminal emission, and removing dampness, and they are the ministerial herbs. Mulberry and raspberry can tonify the kidney and arrest seminal emission, nourish yin and promote the production of body fluid, and they are also the ministerial herbs. Chinese date can supplement qi and nourish blood, and it is also the ministerial herb. The five herbs together can enhance the effect of the sovereign herbs in tonifying the kidney, nourishing yin and promoting the production of body fluid. Semen alpiniae oxyphyllae warms and tonifies the spleen and kidney, arrests seminal emission and reduces urination. Cinnamon supplements fire to assist yang, warms the spleen and dredges the meridian. The combination of the two herbs can moderately warm and tonify to prevent the sovereign and ministerial herbs from being too cold, and it also guides all the herbs to enter the kidney, so they are the adjuvant herbs. Ganoderma lucidum supplements the lung and benefits qi, nourishes the liver and strengthens the spleen, and it is the adjuvant herb. Dried ginger warms the middle-jiao to dispel cold, restores yang and unblocks the pulse, warms the lung and transforms fluid-retention. It neutralizes the cold nature of the above-mentioned herbs, making the whole formula nourish yin without being greasy and not affecting the transportation and transformation functions of the spleen and stomach, and it is the envoy herb of the whole formula. The common use of all the herbs can achieve the effects of tonifying the kidney and replenishing essence, nourishing yin and promoting the production of body fluid.

[0017] Compared with the prior art, the beneficial effects of the present invention are as follows: The present invention provides a medicated diet formula, food, health food, medicine and their preparation methods and uses based on the kidney attribute of the five elements in traditional Chinese medicine. This medicated diet formula is based on the five-element characteristics of the kidney attribute of the five elements in traditional Chinese medicine. Each medicinal flavor complements each other, tonifies the kidney and replenishes essence, nourishes yin and promotes the production of body fluid, and can significantly counteract the immune hypofunction caused by cyclophosphamide and is used to enhance immunity. Moreover, all the raw materials of the medicinal flavors in the present invention are both edible and medicinal, safe and effective, and have good application prospects.

[0018] Obviously, according to the above content of the present invention, and in accordance with the common general technical knowledge and customary means in the art, without departing from the above basic technical idea of the present invention, various other forms of modification, substitution or change can be made.

[0019] The following is a further detailed description of the above content of the present invention through specific embodiments in the form of examples. However, this should not be construed as limiting the scope of the above subject matter of the present invention to the following examples. All technologies implemented based on the above content of the present invention belong to the scope of the present invention. Detailed Embodiments

[0020] The raw materials and equipment used in the present invention are all known products and are obtained by purchasing commercially available products.

[0021] Example 1. Preparation of the Medicated Diet Formula of the Present Invention Formula: 12 g of polygonatum sibiricum, 2 g of cinnamon, 10 g of fresh Chinese yam, 10 g of euryale ferox, 3 g of semen alpiniae oxyphyllae, 10 g of wolfberry fruit, 5 g of mulberry, 5 g of raspberry, 5 g of lotus seed, 10 g of Chinese date, 4 g of dried ginger, and 3 g of ganoderma lucidum.

[0022] Preparation method: Weigh the raw medicinal materials according to the formula, decoct with 6 times the volume of water for 4 times, each time for 2 h after boiling, combine the filtrates, concentrate under normal pressure to 132 mL (0.6 g of crude drug / mL), and store at -20 °C for later use.

[0023] The preparation of the control samples is carried out through the following control examples.

[0024] Control Example 1. Preparation of the medicated diet formula Formula: 12 g of Polygonatum sibiricum, 2 g of Cinnamon, 10 g of raw Chinese yam, 10 g of raw Gorgon fruit, 4 g of Dry Ginger, 3 g of Ganoderma lucidum.

[0025] Preparation method: Weigh the raw medicinal materials according to the formula, decoct with 6 times the volume of water for 4 times, each time for 2 h after boiling, combine the filtrates, concentrate under normal pressure to 68 mL (0.6 g of crude drug / mL), and store at -20 °C for later use.

[0026] Control Example 2. Preparation of the medicated diet formula Formula: 3 g of Alpinia oxyphylla, 10 g of Lycium barbarum, 5 g of Mulberry, 5 g of Rubus idaeus, 5 g of Nelumbo nucifera Gaertn. seeds, 10 g of Chinese date, 3 g of Ganoderma lucidum.

[0027] Preparation method: Weigh the raw medicinal materials according to the formula, decoct with 6 times the volume of water for 4 times, each time for 2 h after boiling, combine the filtrates, concentrate under normal pressure to 68 mL (0.6 g of crude drug / mL), and store at -20 °C for later use.

[0028] Control Example 3. Preparation of the medicated diet formula Formula: 15 g of Polygonatum sibiricum, 2 g of Cinnamon, 12 g of raw Chinese yam, 8 g of raw Gorgon fruit, 3 g of Alpinia oxyphylla, 12 g of Lycium barbarum, 3 g of Mulberry, 3 g of Rubus idaeus, 3 g of Nelumbo nucifera Gaertn. seeds, 8 g of Chinese date, 4 g of Dry Ginger, 3 g of Ganoderma lucidum.

[0029] Preparation method: Weigh the raw medicinal materials according to the formula, decoct with 6 times the volume of water for 4 times, each time for 2 h after boiling, combine the filtrates, concentrate under normal pressure to 127 ml (0.6 g of crude drug / mL), and store at -20 °C for later use.

[0030] Control Example 4. Preparation of the medicated diet formula Formula: 10 g of Polygonatum sibiricum, 2 g of Cinnamon, 8 g of raw Chinese yam, 12 g of raw Gorgon fruit, 3 g of Alpinia oxyphylla, 8 g of Lycium barbarum, 8 g of Mulberry, 8 g of Rubus idaeus, 8 g of Nelumbo nucifera Gaertn. seeds, 12 g of Chinese date, 4 g of Dry Ginger, 3 g of Ganoderma lucidum.

[0031] Preparation method: Weigh the raw medicinal materials according to the formula, decoct with 6 times the volume of water for 4 times, each time for 2 h after boiling, combine the filtrates, concentrate under normal pressure to 144 mL (0.6 g of crude drug / mL), and store at -20 °C for later use.

[0032] Among them, the difference in the formula between Example 1 and Control Examples 1 to 4 lies in: (1)The formula of Control Example 1 is based on Composition 4 (12 g of Polygonatum sibiricum, 2 g of Cinnamon, 10 g of raw Chinese yam, 10 g of raw Gorgon fruit, 4 g of Dry Ginger) disclosed in the patent application document (CN110959841A), and then 3 g of Ganoderma lucidum is added; (2)The formula of Control Example 2 is obtained by subtracting Composition 4 (12 g of Polygonatum sibiricum, 2 g of Cinnamon, 10 g of raw Chinese yam, 10 g of raw Gorgon fruit, 4 g of Dry Ginger) disclosed in the patent application document (CN110959841A) from the formula of Example 1, and the remaining drug composition is the formula of Control Example 2; (3)The formula of Control Example 3 is based on the formula of Example 1, with an increase in the dosage of the monarch drugs (Polygonatum sibiricum, raw Chinese yam, Lycium barbarum) and a decrease in the dosage of the ministerial drugs (raw Gorgon fruit, Mulberry, Rubus chingii, Nelumbo nucifera Gaertn., Chinese date); (4)The formula of Control Example 4 is based on the formula of Example 1, with an increase in the dosage of the ministerial drugs (raw Gorgon fruit, Mulberry, Rubus chingii, Nelumbo nucifera Gaertn., Chinese date) and a decrease in the dosage of the monarch drugs (Polygonatum sibiricum, raw Chinese yam, Lycium barbarum).

[0033] The beneficial effects of the present invention are demonstrated by the following experimental examples.

[0034] Experimental Example 1: Effect of the Medicinal Diet Formula of the Present Invention on Enhancing Immunity 1. Experimental Materials A model of immunosuppression in mice induced by cyclophosphamide was used to compare the results of different medicinal diet formulas of the examples and control examples in enhancing immunity.

[0035] (1)Experimental Animals Kunming mice (KM mice), 70 in number, half male and half female, weighing 18 - 22 g, were purchased from Spf (Beijing) Biotechnology Co., Ltd., and the animal production license number is: SCXK (Jing) 2019 - 0010. The KM mice were raised in the SPF animal laboratory of the Sichuan Academy of Chinese Medicine Sciences, and the animal use license number is: SYXK (Chuan) 2023 - 0100.

[0036] (2)Experimental Drugs Cyclophosphamide for injection was purchased from Baxter Oncology Gmbh, and the approval number for drugs in China is HJ20160467.

[0037] Chinese medicinal materials: Polygonatum sibiricum, Cinnamon, raw Chinese yam, raw Gorgon fruit, Alpinia oxyphylla, Lycium barbarum, Mulberry, Rubus chingii, Nelumbo nucifera Gaertn., Chinese date, Dry Ginger, Ganoderma lucidum, from Sichuan Guoqiang Chinese Medicinal Herb Slices Co., Ltd.

[0038] 2. Experimental Methods Chicken red blood cells are a commonly used immunogen, which can be used to study the generation of hemolysin antibodies in mice, so as to evaluate the immunity of mice. After 3 days of adaptive feeding of the mice, they were randomly divided into 7 groups according to body weight, and the grouping and drug administration are shown in Table 1.

[0039] Table 1. Administration and Dosage of the Effects of Different Medicated Diet Formulas on the Generation of Hemolysin Antibodies in Mice Induced by Chicken Red Blood Cells Note: Compared with the control group: **P<0.01; compared with the model group: △ P <0.05.

[0040] In Comparative Examples 1-4 and Example 1, gavage administration was performed once a day at the dose for 5 consecutive days; the control group and the model group were given the same dose of distilled water once a day for 5 consecutive days. Except for the control group, in Comparative Examples 1-4 and Example 1, 0.5 h after administration on the 5th day, a chicken red blood cell solution was intraperitoneally injected into the mice at 0.2 ml / mouse, and at the same time, a cyclophosphamide saline solution of 20 mg / kg was intraperitoneally injected. Then, on the 7th and 9th days, a cyclophosphamide saline solution of 20 mg / kg was intraperitoneally injected into the mice, and continuous administration was carried out during this period; the model group was given the same dose of distilled water every day. 0.5 h after administration of the same dose of distilled water on the 5th day, a chicken red blood cell solution was intraperitoneally injected into the mice at 0.2 ml / mouse, and at the same time, a cyclophosphamide saline solution of 20 mg / kg was intraperitoneally injected. Then, on the 7th and 9th days, a cyclophosphamide saline solution of 20 mg / kg was intraperitoneally injected into the mice, and the same dose of distilled water was continuously given during this period.

[0041] 0.5 h after gavage administration to the mice on the 11th day of the experiment, 0.025 ml of blood was taken from the posterior orbital venous plexus of the mice with a pointed pipette and added to a test tube containing 1 ml of physiological saline. Then, 0.5 ml of 4% chicken red blood cell solution and 0.5 ml of 15% guinea pig serum were added, and it was placed in a water bath at 37°C for 1 h. After 1 h, the reaction was terminated by ice bath for 10 min, centrifuged at 2000 r / min for 10 minutes, 1 ml of the supernatant was taken and added to 3 ml of hemoglobin application solution, and colorimetric determination was carried out at 540 nm with an enzyme-linked immunosorbent assay instrument. The results are expressed as X±S, and a t-test was performed between groups.

[0042] 3. Experimental Results Table 2. Results of the Effects of Different Black Decoction Medicated Diet Samples on the Generation of Hemolysin Antibodies in Mice Induced by Chicken Red Blood Cells Note: Compared with the control group: **P<0.01; compared with the model group: △△ P <0.01.

[0043] Table 2 shows the results of the effects of different black decoction medicated diet sample formulations on the production of hemolysin antibodies in mice caused by chicken red blood cells. From the results in Table 2, it can be seen that there are significant differences between the black decoction medicated diet formulation of Example 1 and the model group, indicating that it has a significant antagonistic effect on the immune deficiency caused by cyclophosphamide (P<0.01); while there are no significant differences between the black decoction medicated diet formulations of Comparative Examples 1 to 4 and the model group, and the effects of improving immune deficiency of the black decoction medicated diet formulations of Comparative Examples 1 to 4 are not as good as that of the black decoction medicated diet formulation of Example 1. The test results show that under the black decoction medicated diet formulation with specific composition and ratio in the present invention, the level of hemolysin antibody production in immune-deficient model mice can be significantly increased.

[0044] In summary, the present invention provides a medicated diet formulation, food, health food, drug and their preparation methods and uses based on the kidney attribute of the five elements in traditional Chinese medicine. The medicated diet formulation of the present invention is prepared from the following raw materials in the following weight ratios: 10-14 parts of polygonatum sibiricum, 1-3 parts of cinnamon, 8-12 parts of raw yam, 8-12 parts of raw euryale ferox, 2-4 parts of semen carthami, 8-12 parts of wolfberry, 4-6 parts of mulberry, 4-6 parts of raspberry, 4-6 parts of lotus seed, 8-12 parts of jujube, 3-5 parts of dried ginger, 2-4 parts of ganoderma lucidum. Based on the five-element characteristics of the kidney attribute of the five elements in traditional Chinese medicine, the medicated diet formulation of the present invention has the various medicinal flavors complementing each other, tonifying the kidney and replenishing essence, nourishing yin and promoting fluid production, and can significantly antagonize the immune deficiency caused by cyclophosphamide and is used to enhance immunity. Moreover, all the raw materials of the medicinal flavors in the present invention are both medicine and food, safe and effective, and have good application prospects.

Claims

1. A medicinal diet recipe, characterized in that: The medicine is prepared from raw materials in the following weight ratio: 12 parts of polygonatum, 2 parts of cinnamon bark, 10 parts of raw yam, 10 parts of raw euryale, 3 parts of alpinia oxyphylla, 10 parts of wolfberry, 5 parts of mulberry, 5 parts of raspberry, 5 parts of lotus seed, 10 parts of jujube, 4 parts of dried ginger and 3 parts of ganoderma lucidum.

2. The medicinal diet recipe according to claim 1, characterized in that: It is a food or drug preparation prepared by taking the powder of the raw material medicine in the weight ratio, or the water and / or organic solvent extract of the raw material medicine as active ingredients, and adding food or pharmaceutically acceptable auxiliary materials or auxiliary ingredients.

3. A method for preparing the medicinal diet recipe according to any one of claims 1 to 2, characterized in that: The method comprises the following steps: taking raw materials of various weight proportions, directly grinding them into powder, or adding water or organic solvent for extraction, and then adding auxiliary materials or auxiliary ingredients acceptable to food or medicine to obtain food or medicine preparations.

4. Use of the medicinal diet recipe according to any one of claims 1 to 2 in preparing a health food that helps to enhance immunity.

5. Use of the medicinal diet recipe according to any one of claims 1 to 2 in preparing food.

6. Use of the medicinal diet recipe according to any one of claims 1 to 2 in the preparation of a medicine for improving immunity.

7. A food, characterized in that It comprises the medicinal diet recipe described in any one of claims 1 to 2.

8. A health food that helps to enhance immunity, characterized in that: The medicinal food formula is prepared by using the medicinal food formula described in any one of claims 1 to 3 as an active ingredient and adding auxiliary materials or supplementary ingredients acceptable to health food.

9. A drug for improving immunity, characterized in that: The medicinal diet formula is prepared by taking the medicinal diet formula described in any one of claims 1 to 2 as an active ingredient and adding pharmaceutically acceptable auxiliary materials or auxiliary ingredients.

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