Strong growth control fertilizer composition suitable for being applied to soil and preparation method thereof

Abstract

The invention discloses a vigorous growth control fertilizer composition suitable for being applied to soil, which is characterized by comprising the following components in percentage by weight: 40-70% of organic carbon, 1-4% of bacillus subtilis metabolite and the balance of mineral nutrient elements and / or filler, wherein the organic carbon comprises at least one selected from citric acid, malic acid, succinic acid, tartaric acid, L-ascorbic acid, L-sorbose, L-arabinose, galactose and erythritol, and the bacillus subtilis metabolite is prepared by performing low-temperature concentration and drying on bacillus subtilis fermentation liquor which is fermented for 5-10 days. The composition is suitable for being directly applied to soil to achieve a mild, long-acting and stable vigorous growth control effect, coordinates vegetative growth and reproductive growth of crops, can be widely applied to vigorous growth control of fruit trees and various crops, and can improve the yield and quality of the crops.

Description

Technical Field

[0001] The present invention relates to the technical field of fertilizers, and particularly relates to a growth-regulating fertilizer composition suitable for being applied to soil and a preparation method thereof. Background Art

[0002] Crops may exhibit excessive growth due to factors such as climate, fertilization, and hormones. For example, excessive rainfall, over-fertilization or improper fertilization, and unscientific application of plant growth regulators can lead to overly vigorous vegetative growth of crops, which will consume a large amount of nutrients. While extending the vegetative growth period, the nutrient supply for reproductive growth cannot be guaranteed, resulting in many negative factors that are not conducive to high crop yields, such as late ripening of crops, serious flower dropping, low fruit setting rate, slow tuber swelling, and easy lodging of plants. Therefore, it is necessary to control the excessive growth of crops. Common growth-regulating products on the market, such as paclobutrazol, uniconazole, prohexadione-calcium, mepiquat chloride, chlormequat chloride, etc., all belong to plant endogenous hormone inhibitors. Their growth-regulating principle is to control the formation of gibberellin in plants, and the application requirements are relatively high. If used carelessly, it is easy to cause crop yield reduction. Summary of the Invention

[0003] The present application provides a growth-regulating fertilizer composition suitable for being applied to soil. This composition has a low cost and high efficiency, is suitable for direct application to soil, brings a mild, long-lasting, and stable growth-regulating effect, and coordinates the vegetative growth and reproductive growth of crops. The present application includes the following embodiments:

[0004] Embodiment 1. A growth-regulating fertilizer composition suitable for being applied to soil, comprising:

[0005] 40wt%-70wt% organic carbon,

[0006] 1wt%-4wt% Bacillus subtilis metabolites,

[0007] and the balance of mineral nutrient elements and / or fillers;

[0008] wherein, the organic carbon comprises at least one selected from citric acid, malic acid, succinic acid, tartaric acid, L-ascorbic acid, L-sorbose, L-arabinose, galactose, and erythrose,

[0009] The Bacillus subtilis metabolites are prepared by low-temperature concentration and drying of a Bacillus subtilis fermentation broth fermented for 5 to 10 days.

[0010] Embodiment 2. The growth-regulating fertilizer composition according to Embodiment 1, wherein the Bacillus subtilis metabolites are prepared by the following method:

[0011] Inoculate Bacillus subtilis into the sterilized nutrient solution, continuously ferment at 32 - 40 °C for 5 to 10 days, filter to remove the residue, and concentrate and dry the filtrate below 60 °C to obtain the Bacillus subtilis metabolite. The nutrient solution contains a carbon source, a nitrogen source, and inorganic salts.

[0012] Embodiment 3. The growth-regulating fertilizer composition according to Embodiment 1, wherein the mineral nutrient element comprises one or more selected from potassium nitrate, potassium dihydrogen phosphate, potassium chloride, monoammonium phosphate, and diammonium phosphate, and the filler comprises one or more selected from anti-caking agents, diatomaceous earth, talcum powder, zeolite powder, and bentonite.

[0013] Embodiment 4. The growth-regulating fertilizer composition according to Embodiment 1, wherein the organic carbon further comprises a compound of the following Structural Formula I or Structural Formula II.

[0014]

[0015] Embodiment 5. The growth-regulating fertilizer composition according to Embodiment 2, wherein the carbon source comprises one or more selected from molasses, starch, humic acid, glucose, and sucrose; the nitrogen source comprises one or more selected from amino acids, soybean meal, urea, ammonium nitrate, and potassium nitrate; and the inorganic salts comprise one or more selected from potassium dihydrogen phosphate, disodium hydrogen phosphate, potassium chloride, sodium chloride, magnesium sulfate, calcium chloride, and ferrous sulfate.

[0016] Embodiment 6. The growth-regulating fertilizer composition according to Embodiment 2, wherein the carbon-nitrogen ratio in the nutrient solution is 15 - 20:1, and the initial pH value of the culture solution is 6 - 6.8.

[0017] Embodiment 7. A method for preparing the growth-regulating fertilizer composition according to any one of Embodiments 1 to 6, which is prepared by mixing the following raw materials in proportion:

[0018] 40wt% - 70wt% organic carbon,

[0019] 1wt% - 4wt% Bacillus subtilis metabolite,

[0020] and the balance of mineral nutrient elements and / or fillers; the preparation of the compound containing Structural Formula I or Structural Formula II includes a process of selecting 2-methyl-2-pentenoic acid, (R,S)-1,2,3,4-butanetetraol, and borax pentahydrate for reaction.

[0021] Embodiment 8. A method for regulating the growth of fruit trees, applying the growth-regulating fertilizer composition according to any one of Embodiments 1 to 6 1 to 2 times during the fruit swelling period to before fruit picking, the total application amount of the growth-regulating fertilizer composition being 60 - 80 g / mu, and the application method being broadcasting, hole application, or strip application.

[0022] Preferably, apply the growth-regulating fertilizer composition described in Embodiment 7 at a rate of 20 - 40 grams per mu, and the application methods include broadcasting, hole application or band application.

[0023] Embodiment 9. A method for testing the growth-regulating fertilizer composition described in any one of Embodiments 1 to 6, including performance testing of the compound containing the structural formula I or structural formula II or testing the release of concentrated granular boron.

[0024] Embodiment 10. A method for treating crop seeds, using the growth-regulating fertilizer composition described in any one of Embodiments 1 to 6 for seed dressing or seed soaking, and the crops are wheat, rice or corn.

[0025] The composition of the present invention is a powder product prepared by concentrating and drying microbial metabolites and organic carbon, etc. It is suitable for direct application to the soil, coordinates the vegetative growth and reproductive growth of crops, achieves the purpose of balanced growth, and brings a mild, long-lasting and stable growth-regulating effect. Experimental data show that after the composition of the present invention is applied to the soil, it can significantly inhibit the excessive growth of crops, is superior to the existing growth-regulating products in the art, can be widely used for growth regulation of fruit trees and various crops, and can also be used for seed dressing or seed soaking, which can improve the yield and quality of crops. Especially for dwarf and high-density apple trees with extremely high and difficult growth regulation requirements, the composition of this application still has a good growth-regulating effect. Description of the Drawings

[0026] To more clearly illustrate the technical solutions of the embodiments of the present disclosure, the drawings of the embodiments will be briefly introduced below. Obviously, the drawings described below only relate to some embodiments of the present disclosure and do not limit the present disclosure.

[0027] Figure 1 It is the Fourier infrared spectrum of the compound containing the structural formula II. Detailed Embodiments

[0028] To make the purposes, technical solutions and advantages of the embodiments of the present disclosure clearer, the technical solutions of the embodiments of the present disclosure will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present disclosure, rather than all of the embodiments. Based on the described embodiments of the present disclosure, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the scope of protection of the present disclosure.

[0029] In this application, unless specifically pointed out or different meanings can be obtained according to the context, each term has the meaning commonly understood in the art.

[0030] The present invention discloses a growth-regulating fertilizer composition suitable for being applied to soil, comprising: 40wt%-70wt% organic carbon, 1wt%-4wt% metabolites of Bacillus subtilis, and the balance of mineral nutrient elements and / or fillers; wherein, the organic carbon comprises at least one selected from citric acid, malic acid, succinic acid, tartaric acid, L-ascorbic acid, L-sorbose, L-arabinose, galactose, and erythrose, and the metabolites of Bacillus subtilis are prepared by low-temperature concentration and drying of a fermentation broth of Bacillus subtilis fermented for 5 to 10 days. Bacillus subtilis is widely used in the agricultural field, and it has the characteristics of being pollution-free, harmless, and non-pathogenic. It can activate the soil in the soil, and various active substances produced by metabolism can stimulate the growth of crops, promote the release of soil trace elements and plant absorption, reduce costs, increase yields, and increase income. The present application unexpectedly finds that a composition of metabolites of Bacillus subtilis and organic carbon has the effect of delaying plant growth, inhibiting excessive vegetative growth, coordinating the vegetative growth and reproductive growth of crops, thereby achieving growth regulation. The composition prepared in the present application is in powder or granular form, can be directly mixed with other fertilizers and applied to the soil, and is suitable for broadcasting, hole application, and strip application, bringing a mild, long-lasting, and stable growth-regulating effect. In the present application, since both the metabolites of Bacillus subtilis and the bacterial cells themselves are active substances, it is necessary to concentrate and dry them under low-temperature conditions to prepare the growth-regulating fertilizer composition, usually not exceeding 70 degrees Celsius.

[0031] In some embodiments, the metabolites of Bacillus subtilis are prepared by the following method: inoculating Bacillus subtilis into a sterilized nutrient solution, continuously fermenting at 32-40°C for 5 to 10 days, filtering to remove the filter residue, and concentrating and drying the filtrate below 60 degrees Celsius to obtain the metabolites of Bacillus subtilis, and the nutrient solution contains a carbon source, a nitrogen source, and inorganic salts.

[0032] In some embodiments, the mineral nutrient elements include one or more selected from potassium nitrate, potassium dihydrogen phosphate, potassium chloride, monoammonium phosphate, and diammonium phosphate, and the fillers include one or more selected from anti-caking agents, diatomaceous earth, talc powder, zeolite powder, and bentonite.

[0033] In some embodiments, the organic carbon further comprises alkyl acid compounds and ether alcohol compounds. The alkyl acid compounds include one or more of sodium trimethylacetate hydrate, ammonium acetate, ammonium tetradecyl acetate, alkyl sulfonamide, alkyl phosphate ester, and 2-methyl-2-pentenoic acid; the ether alcohol compounds include one or more of (R,S)-1,2,3,4-butanetetraol, chitosan, xylitol, ethylene glycol ether, ethylene glycol vinyl ether, propylene glycol ether, fatty alcohol ether, dodecyl diethylene glycol ether, alcohol ether carboxylic acid, and alcohol ether phosphate salt.

[0034] In some embodiments, the organic carbon includes a compound of Structural Formula I or Structural Formula II as follows

[0035]

[0036] The present application also discloses a preparation method of a compound containing the compounds of Structural Formula I and Structural Formula II;

[0037] The specific preparation method of the compound containing Structural Formula I is as follows:

[0038] Prepare the first reaction solution: sequentially add 15 - 50 parts of (R,S)-1,2,3,4-butanetetraol, 0.5 - 5 parts of 2-methyl-2-pentenoic acid, 0.05 to 5 parts of the catalyst concentrated sulfuric acid, add an appropriate amount of water, heat the solution to 40 - 80 °C and react for 25 - 30 h. Stop heating when sampling shows that no more lipid substances are formed or the pH of the solution no longer rises, and obtain the first reaction solution;

[0039] The preferred process conditions in the above preparation of the first reaction are as follows: the reaction temperature is 65 - 75 °C, the feeding ratio is 30 parts of (R,S)-1,2,3,4-butanetetraol, 5 parts of 2-methyl-2-pentenoic acid, and 0.1 part of the catalyst concentrated sulfuric acid, and react in 5 - 8 kg of water. More preferably, the reaction solution further includes 0.1 part of a promoter in terms of mole fraction, and the promoter is an equimolar mixture of acrylic acid and acetic acid, and the promoter is added after adding concentrated sulfuric acid.

[0040] Prepare the second reaction solution and form the compound containing Structural Formula I: add borax pentahydrate to the first reaction solution, then adjust the pH to 6.9 - 7.2, stir and react at 60 - 90 °C until the borax pentahydrate particles in the reaction solution become clear and disappear, forming the second reaction solution; when the product after drying the second reaction solution is insoluble in ether and hydrochloric acid test, the second reaction solution or its dried product is the compound containing Structural Formula I. The preferred addition ratio in the above process is to add 10 to 12 moles of borax pentahydrate to every 5 kg of the first reaction solution.

[0041] A more preferred process for preparing the second reaction solution is to dry and crush the second reaction solution to prepare grayish-white concentrated particles. For example, take 10 - 100 g of the sample solution of the second reaction solution and dry and crush it into grayish-white concentrated particles of 10 mesh. The concentrated particles are the compound containing Structural Formula I and also a kind of organic carbon, and the particles are insoluble in ether and hydrochloric acid test.

[0042] The specific preparation method of the compound containing Structural Formula II is as follows:

[0043] Maintain the reaction temperature of the second reaction solution at 40 - 85°C, introduce nitrogen gas, add hydrogen peroxide and ammonium bisulfite, and continue the constant-temperature reaction for 10 - 20 hours after the solution viscosity significantly increases to obtain the compound with structural formula II. After drying and pulverizing, aggregated particles are formed, and these aggregated particles are the concentrated compound with structural formula II.

[0044] The specific preferred preparation method of the compound with structural formula II is as follows. At 65°C, after introducing nitrogen gas for 1 hour, add 0.01% hydrogen peroxide and 0.02% ammonium bisulfite by mass percentage. After the solution viscosity significantly increases, continue the constant-temperature reaction for 10 - 20 hours to obtain the compound with structural formula II. After drying and pulverizing, aggregated particles are formed, and these aggregated particles are the concentrated compound with structural formula II and also a kind of organic carbon.

[0045] Furthermore, the compound with structural formula I or II is prepared into immediate-release granules, and the specific method is as follows:

[0046] Add the compound with structural formula I or II to no more than half of the water (containing 3 - 5% ether in the water) to form a viscous paste. Based on the viscous paste, soak 3 - 8% of ammonium carbonate particles or ammonium persulfate particles with a mesh size of 40 - 80 in cottonseed oil and span 80 (the mass ratio of cottonseed oil to span 80 is 20:1) to form coated particles. The coated particles are completely dissolved without complete delamination within 8 hours when stirred in acidic water. Add the coated particles to the viscous paste and stir evenly, and then air-dry and granulate the above viscous paste within 8 hours to form immediate-release granules. The immediate-release granules include immediate-release granules containing structural formula I and immediate-release granules containing structural formula II.

[0047] The following describes the performance test of the organic carbon-containing or the boron release test of the concentrated particles, and the specific method is as follows:

[0048] The test method is as follows: Make the compound with structural formula I into particles with a particle size of 10 mesh, which are grayish-white particles. Weigh 3 g of the concentrated particles and bury them in alkaline soil with a humidity of not less than 20% for 2 months. Preferably, the pH is 7.3, and preferably 300 g of alkaline soil in a coastal area. Before burial, acidify with hydrochloric acid, and test the boron content in the soil with the filtrate to be 70 micrograms / gram, with a humidity of 27%. Bury them for 2 months in an environment of 15 - 23°C, preferably 20°C, and then wash and filter with 80-mesh sieve using ether. A small amount of grayish-white concentrated particles larger than 30 mesh are left with a filtration residue of 1.1 g, indicating that decomposition has occurred. After separating the residue, acidify the soil with hydrochloric acid and then filter, and test the boron content in the acidified filtrate to be about 108 micrograms / gram, indicating that boron release has occurred in the soil under the above conditions. In addition, test the boron content with the above method to be 79 micrograms / gram at 1 month, indicating that the release slightly accelerates with the extension of time.

[0049] If the pH value in the test method conditions is changed from 7.3 to 6, and other test conditions remain unchanged, the boron content is measured to be 71 micrograms per gram after 2 months, and the release shows basically no change, indicating that the release of the concentrated granules is slower in acidic soil. Additionally, if the temperature in the test method is replaced with 50 degrees and other conditions remain unchanged, the boron content is measured to be 106 micrograms per gram after 2 months, indicating that the release rate does not increase or even shows a tendency to slow down within the temperature range of up to 50 degrees. The quick-release granules containing structural formula II also show an accelerated release when tested in acidic water or acidic soil.

[0050] Replace the soil in the test method with tap water, and keep other relevant conditions unchanged. The boron content in tap water is 0.000004 micrograms per gram. After 2 days of testing (the boron ion content was 92 micrograms per gram when tested after 1 day), the boron ion content in the aqueous solution basically reaches about 223 micrograms per gram, indicating that almost all the boron has been released. This shows that the concentrated granules release quickly in the aqueous solution under the test conditions and can be used for fast fertilization. The release rate slightly increases with the passage of time.

[0051] Replace the soil in the test method with tap water, and at the same time replace the concentrated granules with aggregated granules. Keep other relevant conditions unchanged. After 4 days of testing, the boron ion content in the aqueous solution basically reaches about 218 micrograms per gram, indicating that most of the boron has been released. This shows that the release of the concentrated granules is slower in the aqueous solution under the test conditions and can be used for delayed fertilization.

[0052] Replace the concentrated granules in the test method with quick-release granules containing structural formula I, and replace the pH 7.3 alkaline soil with pH 5.8 acidic soil. After 2 months of testing with other test conditions unchanged, the boron content is measured to be 102 micrograms per gram, indicating that the quick-release granules containing structural formula I release faster in acidic soil. It is analyzed that alkalinity and / or oxidability are beneficial to accelerating the release of boron by the organic carbon-containing substances in this application. Similar effects of accelerating the release of boron are obtained for the quick-release granules containing structural formula I in water, the quick-release granules containing structural formula II in water, or in acidic soil through the same above-mentioned test methods. Similar effects of accelerating the release are obtained for the quick-release granules containing structural formula I in water or the quick-release granules containing structural formula II in an environment with pH 7.3 through the same above-mentioned test methods.

[0053] The sample of the aggregated granules containing structural formula II is prepared into a film with potassium bromide through the solution coating method and then analyzed by Fourier transform infrared spectroscopy to obtain a spectrum, as Figure 1As shown, the fewer peaks around 1586 cm-1 of the sample should be due to the opening of the olefin bond. The stretching vibration occurring at 3400 cm to 3500 cm should be the characteristic absorption peak of the (R,S)-1,2,3,4-butanetetraol hydroxyl group; the stretching vibration of the C—O double bond carbonyl appears around 1780 cm, which should be due to the participation of the anhydride in the reaction. The peak near 1300 cm reflects the boron-related effect. The appearance of more absorption in the range of 1063 - 1290 cm indicates the formation of esters. The strong and broad absorption band in the region of 3180 - 3610 cm indicates a relatively high content of hydroxyl functional groups; the absorption peak at 2800 - 2900 cm is for methyl vibration, from which it can be seen that the organic carbon substance with structural formula II is formed, and the test methods and results of the substance with structural formula I are similar to those of the aggregated particle sample.

[0054] In some embodiments, the carbon source includes one or more selected from molasses, starch, humic acid, glucose, and sucrose; the nitrogen source includes one or more selected from amino acids, soybean meal, urea, ammonium nitrate, and potassium nitrate; the inorganic salts include one or more selected from potassium dihydrogen phosphate, disodium hydrogen phosphate, potassium chloride, sodium chloride, magnesium sulfate, calcium chloride, and ferrous sulfate.

[0055] In some embodiments, the carbon-nitrogen ratio in the nutrient solution is 15 - 20:1, and the initial pH value of the culture solution is 6 - 6.8.

[0056] This application also discloses a method for preparing the described plant growth regulator fertilizer composition, which is prepared by mixing raw materials in the following ratios: 40wt% - 70wt% organic carbon, 1wt% - 4wt% metabolites of Bacillus subtilis, and the balance of mineral nutrient elements and / or fillers.

[0057] This application also discloses a method for controlling the growth of fruit trees, which is to apply the described plant growth regulator fertilizer composition 1 to 2 times from the fruit swelling stage to before fruit harvesting.

[0058] In some embodiments, for the described method for controlling the growth of fruit trees, the total application amount of the described plant growth regulator fertilizer composition is 60 - 80 grams per mu, and the application methods are broadcasting, hole application, or strip application.

[0059] This application also discloses a method for controlling the growth of crops, which is to apply the described plant growth regulator fertilizer composition, and the application amount is 20 - 40 grams per mu, and the application methods are broadcasting, hole application, or strip application.

[0060] This application also discloses a method for treating crop seeds, which is to use the described plant growth regulator fertilizer composition for seed dressing or seed soaking, and the crops are wheat, rice, or corn.

[0061] Examples

[0062] The raw materials used in the embodiments of this application are all commercially available products and can be purchased in the market. Among them, Bacillus subtilis is in powder form with an effective viable count of ≥100 billion / g, paclobutrazol is a 15% wettable powder, calcium cyclamate is a 15% wettable powder, and brassinolide is a 0.01% soluble concentrate.

[0063] Example 1

[0064] This example discloses a growth-regulating fertilizer composition A suitable for application to soil, which is prepared by mixing the following raw materials in the following proportions: 40 wt% organic carbon, 4 wt% metabolites of Bacillus subtilis, 30 wt% mineral nutrient elements, and the balance filler.

[0065] Among them, the organic carbon is 50 wt% citric acid and 50 wt% L-arabinose. The metabolites of Bacillus subtilis are prepared as follows: A nutrient solution containing a carbon source, a nitrogen source, and inorganic salts is autoclaved, inoculated with Bacillus subtilis, the pH value is adjusted to 6.5, and continuous aeration and stirring fermentation are carried out. During the fermentation process, the temperature is controlled at 36-38 °C. After 5 days of fermentation, the filter residue is removed by filtration, and the filtrate is concentrated below 60 °C and then dried to obtain the metabolites of Bacillus subtilis; among them, the carbon source is molasses, the nitrogen source is soybean meal, the total addition amount of soybean meal and molasses is 100 g / L, the carbon-nitrogen ratio is 20:1, the inorganic salts include potassium dihydrogen phosphate (5 g / L), sodium chloride (2 g / L), magnesium sulfate (3 g / L), calcium chloride (3 g / L), ferrous sulfate (3 g / L), the mineral nutrient element is potassium dihydrogen phosphate; the filler is 70 wt% zeolite powder and 30 wt% anti-caking agent.

[0066] Example 2

[0067] This example discloses a growth-regulating fertilizer composition B suitable for application to soil, which is prepared by mixing the following raw materials in the following proportions: 70 wt% organic carbon, 1 wt% metabolites of Bacillus subtilis, 20 wt% mineral nutrient elements, and the balance filler.

[0068] Among them, the organic carbon is 50 wt% malic acid, 30 wt% L-ascorbic acid, and 20 wt% L-sorbose. The metabolite of Bacillus subtilis is prepared by the following method: A nutrient solution containing a carbon source, a nitrogen source, and inorganic salts is autoclaved, inoculated with Bacillus subtilis, the pH value is adjusted to 6.5, and continuous aeration and stirring fermentation are carried out. During the fermentation process, the temperature is controlled at 36-38 °C. After 5 days of fermentation, the filter residue is removed by filtration, and the filtrate is concentrated below 60 °C and dried to obtain the metabolite of Bacillus subtilis; wherein the carbon source is humic acid and glucose, the nitrogen source is urea, the total addition amount of the carbon source and the nitrogen source is 100 g / L, the carbon-nitrogen ratio is 20:1, and the inorganic salts include disodium hydrogen phosphate (5 g / L), potassium chloride (3 g / L), sodium chloride (2 g / L), magnesium sulfate (3 g / L), calcium chloride (3 g / L), ferrous sulfate (3 g / L). The mineral nutrient element is potassium dihydrogen phosphate; the filler is 50 wt% bentonite and 30 wt% talc powder.

[0069] Example 3

[0070] This example discloses a growth-regulating fertilizer composition C suitable for application to soil. Its raw material ratio is the same as that in Example 1. Among them, the metabolite of Bacillus subtilis is obtained by inoculating Bacillus subtilis into a sterilized nutrient solution and continuously fermenting for 10 days, followed by concentration and drying to obtain the growth-regulating fertilizer composition C.

[0071] Example 4

[0072] This example discloses a growth-regulating fertilizer composition D suitable for application to soil, which is prepared by directly mixing all raw materials according to the raw material ratio in Example 2. That is, it does not contain the metabolite of Bacillus subtilis, but directly mixes the unfermented Bacillus subtilis bacterial agent, carbon source, nitrogen source, inorganic salts, organic carbon, mineral nutrient element, and filler to obtain the growth-regulating fertilizer composition D.

[0073] Example 5

[0074] This example discloses a growth-regulating fertilizer composition E suitable for application to soil. The preparation method is the same as that in Example 1, except that the organic carbon is 25 wt% citric acid, 25 wt% L-arabinose, 25 wt% compound with structural formula I, and 25 wt% compound with structural formula II.

[0075] Application test

[0076] The growth-regulating fertilizer compositions prepared in Examples 1 to 5 (Composition A, Composition B, Composition C, Composition D, and Composition E, respectively) were applied to citrus, potatoes, and dwarf high-density apples, and were used for seed dressing or seed soaking on wheat, rice, and corn. In the application tests of this application, plots with relatively high soil fertility were selected, and the water and fertilizer conditions were good during the planting process, without water or fertilizer shortage.

[0077] Experimental Example 1

[0078] For citrus in the fourth year of production, Composition A and Composition B were applied by foliar spraying and soil fertilization respectively, and a control was set. The field management measures and other fertilizer application plans were exactly the same among different treatments and the control. The growth of fruit trees and the yield were observed.

[0079] The test treatments were as follows:

[0080] Treatment 1-1: Spray Composition A. The application method was to spray a 3000-fold dilution on the leaf surface during the fruit swelling period, and then spray a 2000-fold dilution 40 days before harvest. The total application amount for the two times was 60 g / mu.

[0081] Treatment 1-2: Apply Composition A. The application method was to apply it by strip application during the fruit swelling period and 40 days before harvest respectively. The total application amount for the two times was 60 g / mu.

[0082] Treatment 2-1: Spray Composition B. The application method was to spray a 3000-fold dilution on the leaf surface during the fruit swelling period, and then spray a 2000-fold dilution 40 days before harvest. The total application amount for the two times was 80 g / mu.

[0083] Treatment 2-2: Apply Composition B. The application method was to apply it by strip application during the fruit swelling period and 40 days before harvest respectively. The total application amount for the two times was 80 g / mu.

[0084] Control Group 1: Spray paclobutrazol according to the product instruction.

[0085] Control Group 2: Apply paclobutrazol by strip application according to the product instruction.

[0086] Blank control: Do not apply the growth-regulating fertilizer.

[0087] Application effect: Using the composition of this application, it has an obvious effect of controlling shoots and promoting flower bud formation, can significantly promote flower bud differentiation, increase sugar content, color, and fruit swelling, the fruits are bright, and the high-quality fruits increase. The yield per mu of the experimental groups was all above 2000 kg, with an average increase in production of about 8%-10% compared with the control group and an increase in production of about 30% compared with the blank control.

[0088] Experimental Example 2

[0089] In potato cultivation, Composition A and Composition B were applied by foliar spraying and soil fertilization respectively, and a control was set. The field management measures and other fertilizer application schemes were exactly the same among different treatments and the control. The growth and yield of potatoes were observed.

[0090] The experimental treatments were as follows:

[0091] Treatment 1-1: Spray Composition A. The application method was to spray a 3000-fold dilution on the leaves during the vegetative growth stage, with a total of two sprays, and the interval between the two sprays was about 10 days.

[0092] Treatment 1-2: Apply Composition A. The application method was to apply it twice from the vegetative growth stage to before harvesting by broadcasting, and the total application amount for the two times was 30 g / mu.

[0093] Treatment 2-1: Spray Composition B. The application method was to spray a 3000-fold dilution on the leaves during the vegetative growth stage, with a total of two sprays, and the interval between the two sprays was about 10 days.

[0094] Treatment 2-2: Apply Composition B. The application method was to apply it twice from the vegetative growth stage to before harvesting by broadcasting, and the total application amount for the two times was 30 g / mu.

[0095] Control group: Spray prohexadione-calcium on the leaves according to the product instruction.

[0096] Application effect:

[0097] Applying the composition of the present application can make the root system developed, the stem stout, and promote reproductive transformation and the formation of potato tubers. Compared with applying prohexadione-calcium, applying the composition of the present application can significantly reduce the hollowness and cracking of potato tubers caused by more water, and the quality of potatoes is significantly improved.

[0098] Experimental Example 3

[0099] The compositions A, B, C, and D prepared in Examples 1 to 4 were respectively applied to wheat seed dressing, and brassinolide and equal amount of clear water were used for seed dressing as a control.

[0100] Among them, the seed dressing method for Compositions A, B, C, and D was: for every 10 kg of wheat seeds, spray and mix the wheat grains with a 5000-fold dilution, slightly spread out and air dry, then heap and seal for 3 hours, and then sow.

[0101] The seed dressing method for brassinolide was: for every 10 kg of wheat seeds, use 2 g of 0.01% brassinolide, add 500 ml of water, dilute evenly and then carry out seed dressing, evenly coat on the surface of the seeds, and dry in the shade before sowing.

[0102] Compared with the blank control of soaking seeds with clear water, soaking seeds with 0.01% brassinolide and compositions A, B, C, and D can promote early seedling emergence, make the seedlings stronger, and significantly improve the cold and drought resistance of the seedlings. Applying the composition of the present application can further increase the tiller number before winter, improve the ear-bearing rate, and the mu yield increases by more than 10% compared with the control of applying 0.01% brassinolide.

[0103] Experimental Example 4

[0104] The compositions A, B, C, and D prepared in Examples 1 to 4 were respectively applied to rice seed soaking, and brassinolide was used as a control.

[0105] Among them, the seed soaking methods for compositions A, B, C, and D are: soaking seeds in a 2500-fold dilution for 36 - 48 hours and then germinating.

[0106] The seed soaking method for brassinolide is: soaking seeds with a 500-fold dilution of 0.01% brassinolide.

[0107] Compared with the control group, applying the composition of the present application can cultivate multi-tillered short and strong seedlings, without seedling death after transplanting, promote early tillering and early ear formation, increase the number of ears and grains, and the average yield increase is about 8%.

[0108] Experimental Example 5

[0109] The compositions A, B, C, and D prepared in Examples 1 to 4 were respectively applied to corn seed soaking, and brassinolide seed dressing was used as a control.

[0110] Among them, the seed soaking methods for compositions A, B, C, and D are: soaking seeds in a 2500-fold dilution for 12 to 15 hours.

[0111] The seed dressing method for brassinolide is: soaking seeds with a 500-fold dilution of 0.01% brassinolide.

[0112] Compared with the control group, applying the composition of the present application can improve the germination rate and germination potential, effectively control the excessive growth of plants during the reproductive growth stage of corn, improve the yield and quality of corn, and the yield increase rate reaches 15%.

[0113] Experimental Example 6

[0114] For dwarf and dense planting apples with a plant spacing of 1 meter and a row spacing of 3 meters, the compositions A, B, and E of the present application and paclobutrazol were respectively used for the test of controlling excessive growth. At the same time, conventional pruning and girdling were used to control excessive growth, and no other growth control fertilizers were applied as a blank control.

[0115] The application methods and application effects of each treatment are as follows:

[0116] Treatment 1-1: Spraying Composition A: Spraying a 3000-fold dilution on the leaf surface during the fruit swelling period, and then spraying a 2000-fold dilution 40 - 50 days before harvest. The total application amount for the two times is 60 grams per mu.

[0117] Treatment 1-2: Applying Composition A, the application method is to apply it respectively during the fruit swelling period and 40 - 50 days before harvest, and the application method is strip application. The total application amount for the two times is 60 grams per mu.

[0118] Treatment 2-1: Spraying Composition B, the application method is to spray a 3000-fold dilution on the leaf surface during the fruit swelling period, and then spray a 2000-fold dilution 40 - 50 days before harvest. The total application amount for the two times is 80 grams per mu.

[0119] Treatment 2-2: Applying Composition B, the application method is to apply it respectively during the fruit swelling period and 40 - 50 days before harvest, and the application method is strip application. The total application amount for the two times is 80 grams per mu.

[0120] Treatment 3-1: Spraying Composition E, the application method is to spray a 3000-fold dilution on the leaf surface during the fruit swelling period, and then spray a 2000-fold dilution 40 - 50 days before harvest. The total application amount for the two times is 80 grams per mu.

[0121] Treatment 3-2: Applying Composition E, the application method is to apply it respectively during the fruit swelling period and 40 - 50 days before harvest, and the application method is strip application. The total application amount for the two times is 80 grams per mu.

[0122] Control Group 1: Spraying paclobutrazol: Spraying a 1500-fold dilution on the leaf surface during the fruit swelling period, and then spraying a 1500-fold dilution 40 - 50 days before harvest.

[0123] Control Group 2: Applying paclobutrazol: Applying it respectively during the fruit swelling period and 40 - 50 days before harvest, and the application method is strip application.

[0124] Blank Control: Conventional pruning and girdling to control excessive growth, without applying other growth control fertilizers.

[0125] Application Effect:

[0126] For the treatments of applying Composition A and B, the growth of spring shoots and autumn shoots slows down, with a certain growth control effect and no obvious impact on flower bud differentiation. The fruits are large and uniform, with thin peels and a crispy and sweet taste, and the average fruit diameter is above 75mm.

[0127] For the treatment of applying Composition E, the growth of spring shoots and autumn shoots is significantly inhibited, with an obvious growth control effect, an increase in the formation of high-quality flower buds, and the flower quantity in spring increases by more than 35%. The fruits are large and uniform, with thin peels and a crispy and sweet taste, and the average fruit diameter is above 85mm.

[0128] After treatment with paclobutrazol, the growth of spring and autumn shoots slows down, showing a certain effect of controlling excessive growth, and has no obvious impact on flower bud differentiation. The fruits are large and uniform, with thin peels and a crispy and sweet taste, and the average fruit diameter is over 75 mm.

[0129] Blank control: The effect of controlling excessive growth is not obvious, the excessive growth is relatively serious, the uniformity of fruit size is poor, there are more fruits below 70 mm, and the taste of the fruits is poor.

[0130] The above data indicate that composition E has a very good effect of controlling excessive growth on dwarf and high-density apple trees, far superior to the schemes of applying compositions A, B and paclobutrazol.

[0131] The above description is only an exemplary embodiment of the present disclosure and is not intended to limit the protection scope of the present disclosure. The protection scope of the present disclosure is determined by the appended claims.

Claims

1. A growth control fertilizer composition suitable for application to soil, characterized in that: include: 40wt%-70wt% organic carbon, 1wt%-4wt% Bacillus subtilis metabolites, and balance of mineral nutrients and / or fillers; Wherein, the organic carbon comprises at least one selected from citric acid, malic acid, succinic acid, tartaric acid, L-ascorbic acid, L-sorbose, L-arabinose, galactose, and erythrose, The Bacillus subtilis metabolites are prepared by low-temperature concentration and drying of the Bacillus subtilis fermentation liquid fermented for 5 to 10 days.

2. The growth control fertilizer composition according to claim 1, characterized in that: The Bacillus subtilis metabolites are prepared as follows: Bacillus subtilis is inoculated into a sterilized nutrient solution, and fermented at 32-40°C for 5 to 10 days, and then the residue is removed by filtration. The filtrate is concentrated below 60°C and then dried to obtain the Bacillus subtilis metabolite, wherein the nutrient solution contains a carbon source, a nitrogen source and an inorganic salt.

3. The growth control fertilizer composition according to claim 1, characterized in that: The mineral nutrient elements include one or more selected from potassium nitrate, potassium dihydrogen phosphate, potassium chloride, monoammonium phosphate, and diammonium phosphate, and the filler includes one or more selected from anti-caking agents, diatomaceous earth, talcum powder, zeolite powder, and bentonite.

4. The growth control fertilizer composition according to claim 1, characterized in that: The organic carbon also includes compounds of the following structural formula I or structural formula II, 5. The growth control fertilizer composition according to claim 2, characterized in that: The carbon source comprises one or more selected from molasses, starch, humic acid, glucose, and sucrose; the nitrogen source comprises one or more selected from amino acids, soybean meal, urea, ammonium nitrate, and potassium nitrate; and the inorganic salt comprises one or more selected from potassium dihydrogen phosphate, disodium hydrogen phosphate, potassium chloride, sodium chloride, magnesium sulfate, calcium chloride, and ferrous sulfate.

6. The growth control fertilizer composition according to claim 2, characterized in that: The carbon-nitrogen ratio in the nutrient solution is 15-20:1, and the initial pH value of the culture solution is 6-6.

8.

7. A method for preparing the growth-control fertilizer composition according to any one of claims 1 to 6, characterized in that: The following raw materials are mixed: 40wt%-70wt% organic carbon, 1wt%-4wt% Bacillus subtilis metabolites, and the remainder of mineral nutrients and / or fillers; the preparation of the compound containing structural formula I or II includes the process of selecting 2-methyl-2-pentenoic acid, (R, S)-1,2,3,4-butanetetrol, and borax pentahydrate for reaction.

8. A method for controlling the growth of fruit trees, characterized in that: The growth-control fertilizer composition according to any one of claims 1 to 6 is applied 1 to 2 times from the fruit swelling period to before the fruit is picked. The total application amount of the growth-control fertilizer composition is 60-80 g / mu, and the application method is broadcasting, hole application or strip application.

9. A method for treating crop seeds, characterized in that: The growth-control fertilizer composition according to any one of claims 1 to 6 is used for seed dressing or seed soaking, and the crop is wheat, rice or corn.

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