Microorganism real-time detection device based on CO2 sensor

Abstract

The present invention discloses a CO2 sensor-based microorganism real-time detection apparatus, which comprises a CO2 detector and a base, the top of the base is provided with a horizontally distributed spiral liquid pipe, the two ends of the spiral liquid pipe are provided with connection pipes coaxial with a spiral shaft, the top of the base is fixedly connected with a support plate rotatably connected with the connection pipes, and the support plate is fixedly connected with the CO2 detector. One end of the connecting pipe is connected with a pipe valve, the peripheral wall of the pipe valve is fixedly connected with a sterile turnover cylinder, and the pipe valves located at the two ends of the spiral liquid pipe are jointly connected with a positive and negative pressure pipe. According to the device disclosed by the invention, the spiral liquid pipe, the positive and negative pressure pipe, the CO2 detector and the control combination are arranged, so that a culture solution containing microorganisms in the spiral liquid pipe can continuously flow when the spiral liquid pipe rotates, and when the culture solution flows, gas in the spiral liquid pipe can be pushed to flow in an accelerated manner and passes through the positive and negative pressure pipe containing the CO2 detector; by means of the arrangement, carbon dioxide generated by microbial activity can be rapidly detected in time in real time, and the accuracy of microbial detection is greatly improved.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial detection, and particularly to a real-time microbial detection device based on a CO2 sensor. Background Art

[0002] Microorganisms generally include biological groups such as bacteria, viruses, fungi, and some small protists in a broad sense. Their individuals are extremely tiny, most of which are invisible to the naked eye, and they are closely related to humans. Microbial detection is often required in the detection of current food, medicine, and environment. Since microorganisms continuously oxidize energy organic matter into carbon dioxide during growth and metabolism, releasing chemical energy or metabolic energy and other by-products, technologies including detecting changes in the concentration of microbial by-products, such as detecting changes in carbon dioxide or acid, are used to highlight the contrast relationship between the production of microbial by-products and the dynamic growth process. And by using light with a specific wavelength and a corresponding light sensor to record this dynamic change process, microorganisms can be detected, and the growth dynamics of microorganisms can be recorded, the active state of microorganisms can be judged, and then they can be quantified.

[0003] Currently, the detection devices for detecting carbon dioxide using a CO2 sensor mainly include a detection box. The detection box is internally provided with a culture solution container and a CO2 sensor. During detection, a culture solution containing microorganisms is injected into the culture solution container, and the CO2 sensor transmits the detected carbon dioxide data to an external controller. The controller analyzes and converts it and then outputs the carbon dioxide data and its changing state through a display. However, such detection methods still have deficiencies: 1. The carbon dioxide generated during the activities of microorganisms in the culture solution has poor fluidity, resulting in inaccurate real-time data of the actual activities of microorganisms and the carbon dioxide detected by the CO2 sensor. That is to say, there is a problem of detection lag, resulting in low precision of the actual detection; 2. When injecting the culture solution into the culture solution container, external gas is easily mixed into the detection box, which will not only contaminate the culture solution, but also affect the purity detected by the CO2 sensor, resulting in an error between the detected data and the real data; 3. It does not have the function of quickly adjusting the temperature for detection, and it is difficult to efficiently obtain the activity state of microorganisms at different temperatures by quickly changing the culture temperature, having the defect of low detection efficiency.

[0004] Therefore, the present invention proposes a real-time microbial detection device based on a CO2 sensor. Summary of the Invention

[0005] The purpose of the present invention is to propose a real-time microbial detection device based on a CO2 sensor in order to solve the problems mentioned in the background art.

[0006] In order to achieve the above purpose, the present invention adopts the following technical solutions: A real-time microbial detection device based on a CO sensor, comprising a CO2 detector and a base. A horizontally distributed spiral liquid pipe is arranged on the top of the base. Connecting pipes coaxial with the spiral shaft are arranged at both ends of the spiral liquid pipe. A support plate rotatably connected to the connecting pipes is fixedly connected to the top of the base. One end of the connecting pipe is connected to a pipe valve. A sterile turnover cylinder is fixedly connected to the outer peripheral wall of the pipe valve. The pipe valves at both ends of the spiral liquid pipe are jointly connected to a positive and negative pressure pipe. The pipe valve has the function of controlling the simultaneous connection, non-simultaneous connection, and blockage of the sterile turnover cylinder and the positive and negative pressure pipe with the spiral liquid pipe. The CO2 detector is arranged in the positive and negative pressure pipe. A control combination for controlling the rotation of the spiral liquid pipe and a support for supporting the positive and negative pressure pipe are arranged on the base.

[0007] As a further description of the above technical solution: The sterile turnover cylinder includes a cylinder and a plug disk. One end of the cylinder is fixedly connected to a transfer pipe connected to the pipe valve and its other end is open. The plug disk is sleeved in the cylinder and is in sealed sliding fit. A rubber plug is arranged through the middle of the plug disk.

[0008] As a further description of the above technical solution: The pipe valve includes a valve pipe, a plunger sleeved in the valve pipe, and an electric push rod for controlling the sliding of the plunger. One end of the valve pipe is sleeved in the connecting pipe and is in rotational sealing connection. The outer peripheral wall of the valve pipe is fixedly connected to one end of the positive and negative pressure pipe and their inner cavities are communicated. One end of the transfer pipe is connected to a position on the outer peripheral wall of the valve pipe close to the free end and their inner cavities are communicated. A channel is arranged at one end of the plunger. A first hole and a second hole axially distributed and both communicated with the channel are arranged on the outer peripheral wall of the plunger. The electric push rod is fixedly arranged on one side of the valve pipe.

[0009] As a further description of the above technical solution: The positive and negative pressure pipe is in a U shape, and a transfer cylinder is connected in series on the horizontal pipe thereof. An air extraction pipe is fixedly connected to one end of the transfer cylinder, and an air inlet pipe is fixedly connected to the other end. A discharge pipe close to the mouth is fixedly connected to the outer peripheral wall of the cylinder.

[0010] As a further description of the above technical solution: The support includes a cylinder, a connecting frame, a control motor, a transmission gear, and a transmission rack. One end of the upper cylinder sleeve of the cylinder is hinged to one side of the base. The free end of the piston rod of the cylinder is rotatably connected to the horizontal pipe through the connecting frame. The control motor is fixedly arranged on one side of the cylinder sleeve and its output shaft is fixedly connected to the transmission gear. The transmission rack meshes with the transmission gear and one end of it is fixedly connected to the outer wall of the piston rod. A short connecting pipe communicating with the inner cavity is fixedly connected to the outer peripheral wall of the cylinder sleeve. The short connecting pipe is communicated with the air inlet pipe through a pipeline.

[0011] As a further description of the above technical solution: The control combination includes a passive pulley, an active pulley, a transmission belt, a transmission shaft and a driving motor. The passive pulley is fixedly sleeved outside one of the connecting pipes. The transmission shaft is rotatably connected to one of the support plates and an active pulley is fixedly sleeved thereon. The active pulley is connected to the passive pulley through the transmission belt. The driving motor is fixedly arranged on the top of the base and the output shaft thereof is fixedly connected to one end of the transmission shaft.

[0012] As a further description of the above technical solution: The transmission shaft is rotatably connected to both support plates. A positioning block is screwed on the transmission shaft. An arc-shaped heating plate located below the spiral liquid pipe is fixedly connected to the top of the positioning block. A guide shaft slidably connected to the positioning block is fixedly connected between the two support plates.

[0013] As a further description of the above technical solution: Radiating fans located on both sides of the arc-shaped heating plate are fixedly connected to both sides of the positioning block.

[0014] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are as follows: 1. In the present invention, by arranging the spiral liquid pipe, the positive and negative pressure pipes, the CO2 detector and the control combination, when the spiral liquid pipe rotates, the culture solution containing microorganisms therein will continuously flow. When the culture solution flows, it can promote the gas in the spiral liquid pipe to flow faster and pass through the positive and negative pressure pipes containing the CO2 detector. This setting can quickly and timely detect the carbon dioxide generated by the activities of microorganisms in real time, greatly improving the accuracy of microorganism detection.

[0015] 2. In the present invention, by arranging the pipe valve and the sterile turnover cylinder, and cooperating with the negative pressure environment provided by the positive and negative pressure pipes, the culture solution can automatically and aseptically enter the spiral liquid pipe completely, and the oxygen can automatically fill the spiral liquid pipe completely, so that the raw materials in the spiral liquid pipe are only the culture solution and oxygen, without causing dirt. Therefore, the purity of microorganism culture is greatly improved, and further the purity of microorganism detection is improved.

[0016] 3. In the present invention, by arranging a synchronous heater and radiating fans below the spiral liquid pipe, it is convenient to timely provide different culture temperatures to the spiral liquid pipe, greatly improving the detection efficiency of microorganisms at different temperatures.

[0017] 4. In the present invention, by providing a bracket for supporting the positive and negative pressure pipes, which is composed of a cylinder, a control motor, a transmission gear, a transmission pulley and an adapter, it is convenient to supply positive pressure gas into the spiral liquid pipe through the positive and negative pressure pipes. In cooperation with the opening position of the pipe valve, it is convenient to perform positive pressure jet flushing on the parts through which the microbial culture solution flows, which has the advantages of more thorough cleaning and higher efficiency, and does not affect the detection of other microorganisms. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 is a schematic structural diagram of a real-time microbial detection device based on a CO2 sensor proposed by the present invention; Figure 2 Figure 1 Rear view; Figure 3 is Figure 1 Schematic diagram of the back; Figure 4 is Figure 3 Schematic diagram of the enlarged partial "a" in Figure 5 is Figure 3 Schematic diagram of the enlarged partial "b" in Figure 6 is a schematic structural diagram of a bracket of a real-time microbial detection device based on a CO2 sensor proposed by the present invention.

[0019] LEGEND DESCRIPTION: 1. CO2 detector; 2. Base; 21. Support plate; 211. Guide shaft; 3. Spiral liquid pipe; 4. Connecting pipe; 5. Pipe valve; 51. Valve pipe; 52. Plunger; 521. Channel; 522. First orifice; 523. Second orifice; 54. Electric push rod; 6. Sterile turnover cylinder; 61. Cylinder; 611. Adapter pipe; 612. Discharge pipe; 62. Plug disc; 621. Rubber stopper; 7. Positive and negative pressure pipes; 71. Horizontal pipe; 711. Adapter cylinder; 7111. Suction pipe; 7112. Intake pipe; 8. Control combination; 81. Driven pulley; 82. Driving pulley; 83. Transmission belt; 84. Transmission shaft; 85. Driving motor; 9. Bracket; 91. Cylinder; 911. Cylinder sleeve; 9111. Short connecting pipe; 912. Plug shaft; 92. Adapter; 93. Control motor; 94. Transmission gear; 95. Transmission rack; 101. Positioning block; 102. Arc heating plate; 103. Cooling fan. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0020] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Example

[0021] See also Figures 1-6 The microorganism real-time detection device based on CO2 sensor includes a CO2 detector 1 and a base 2. The CO2 detector 1 has a built-in carbon dioxide sensor for detecting carbon dioxide produced by microorganisms, and the microorganisms are detected based on carbon dioxide and its production.

[0022] In the present technical solution, a horizontally distributed spiral liquid tube 3 is provided on the top of the base 2, and the spiral liquid tube 3 is used to place the microbial culture solution to be detected. Connecting tubes 4 coaxial with the spiral axis are provided at both ends of the spiral liquid tube 3. A support plate 21 rotatably connected to the connecting tube 4 is fixedly connected to the top of the base 2. When in use, the liquid level of the culture solution is lower than the height of the axis of the connecting tube 4. When the spiral liquid tube 3 rotates, the culture solution therein will flow. When the culture solution flows, airflow will be formed at both ends of the culture solution in the spiral liquid tube 3, and the flow state of the culture solution is convenient for comprehensive combination with oxygen, thereby improving the culture effect.

[0023] Furthermore, a pipe valve 5 is connected to one end of the connecting pipe 4, and one connecting pipe 4 is correspondingly connected to one pipe valve 5. The outer peripheral wall of the pipe valve 5 is fixedly connected to a sterile rotating cylinder 6, which is a container for conveying materials (culture fluid and oxygen) into the spiral liquid tube 3, wherein the pipe valve 5 is used to control whether the sterile rotating cylinder 6 is connected to the connecting pipe 4. It should be noted that in this embodiment, the number of the sterile rotating cylinders 6 is two, one for conveying culture fluid, and the other for conveying oxygen, and the conveying is sterile.

[0024] Specifically, the sterile revolving cylinder 6 includes a cylinder 61 and a plug disc 62. One end of the cylinder 61 is fixedly connected to a transfer tube 611 connected to the pipe valve 5 and the other end is open. The plug disc 62 is sleeved in the cylinder 61 and is in a sealed sliding fit. A rubber plug 621 is provided through the middle of the plug disc 62. The closed inner cavity between the plug disc 62 and the cylinder 61 is the raw material turnover cavity. The rubber plug 621 is a component for inserting an injection needle. For example, when it is necessary to inject culture fluid into the raw material turnover cavity, the needle is passed through the rubber plug 621 and then injected. After the liquid enters the raw material turnover cavity, the plug disc 62 will passively slide toward the opening of the cylinder 61. Similarly, oxygen can be added to another sterile revolving cylinder 6 in this way.

[0025] Among them, the pipe valves 5 at both ends of the spiral liquid pipe 3 are jointly connected to a positive and negative pressure pipe 7. The positive and negative pressure pipe 7 is a pipe that provides a negative pressure and positive pressure environment inside the spiral liquid pipe 3. The negative pressure environment is to extract the air inside the spiral liquid pipe 3 to eliminate the influence of the gas inside the spiral liquid pipe 3 on the microbial culture. The positive pressure environment is to provide a high air pressure inside the spiral liquid pipe 3 to facilitate flushing and cleaning the inner cavity of the spiral liquid pipe 3 and improve the cleaning degree of the cleaning.

[0026] Specifically, the positive and negative pressure pipe 7 is in a gate shape, and a transfer cylinder 711 is connected in series on the horizontal pipe 71 thereof. One end of the transfer cylinder 711 is fixedly connected to an air extraction pipe 7111, and the other end is fixedly connected to an air inlet pipe 7112. A discharge pipe 612 is fixedly connected to the outer peripheral wall of the cylinder 61 near the mouth. When the plug disk 62 moves to the opening of the cylinder 61, the discharge pipe 612 is communicated with the raw material turnover cavity inside the cylinder 61. During use, the air extraction pipe 7111 is connected to an external vacuum pump through a pipeline to facilitate vacuum pumping of the spiral liquid pipe 3, and the discharge pipe 612 is connected to an external waste pipe through a pipeline to facilitate pressure discharge of the mixture of the cleaning liquid and the culture solution inside the spiral liquid pipe 3.

[0027] Furthermore, the pipe valve 5 has the function of controlling the simultaneous connection, non-simultaneous connection and blockage of the sterile turnover cylinder 6 and the positive and negative pressure pipe 7 with the spiral liquid pipe 3. When connected simultaneously, it is convenient to vacuum exhaust the spiral liquid pipe 3 and inflate the spiral liquid pipe 3; when not connected simultaneously, it is convenient to transport oxygen and the culture solution into the spiral liquid pipe 3 and facilitate the detection of microorganisms in the culture solution.

[0028] Specifically, the pipe valve 5 includes a valve pipe 51, a plunger 52 sleeved inside the valve pipe 51, and an electric push rod 54 for controlling the sliding of the plunger 52. The plunger 52 and the valve pipe 51 are in a sealed fit structure. One end of the valve pipe 51 is sleeved inside the connecting pipe 4 and is in a rotationally sealed connection. The inner cavity of the valve pipe 51 is communicated with the connecting pipe 4. The outer peripheral wall of the valve pipe 51 is fixedly connected to one end of the positive and negative pressure pipe 7 and their inner cavities are communicated. One end of the adapter pipe 611 is connected to a position on the outer peripheral wall of the valve pipe 51 near the free end and their inner cavities are communicated. When the plunger 52 axially slides inside the valve pipe 51, it can control the on-off of the adapter pipe 611, the positive and negative pressure pipe 7 and the connecting pipe 4. In specific implementation, preferably, a channel 521 is provided at one end of the plunger 52. One end of the channel 521 faces the connecting pipe 4. Axially distributed pore channels 522 and 523 that are both communicated with the channel 521 are provided on the outer peripheral wall of the plunger 52. When the pore channel 522 faces the adapter pipe 611, the channel 521 is communicated with the adapter pipe 611, and the positive and negative pressure pipe 7 is blocked by the plunger 52 and is not communicated with the valve pipe 51. When the pore channel 523 is communicated with the adapter pipe 611, both the adapter pipe 611 and the positive and negative pressure pipe 7 are communicated with the connecting pipe 4. When the pore channels 522 and 523 are located between the adapter pipes 611, both the adapter pipe 611 and the positive and negative pressure pipe 7 are blocked at the same time. When the pore channels 522 and 523 are located on the same side of the adapter pipe 611 and are close to the outer end of the valve pipe 51, the adapter pipe 611 is blocked, and the positive and negative pressure pipe 7 is communicated with the valve pipe 51. The electric push rod 54 is fixedly arranged on one side of the valve pipe 51. The output shaft of the electric push rod 54 is fixedly connected to the other end of a transmission rod arranged on the plunger 52, aiming to control the axial movement of the plunger 52.

[0029] The CO2 detector 1 is arranged inside the positive and negative pressure pipe 7. After the carbon dioxide generated by the microorganisms flows into the positive and negative pressure pipe 7, it is detected by the CO2 detector 1, and then the detection data is transmitted to an external controller and displayed through a display after conversion, showing the data and change amount of carbon dioxide. Due to the rotation of the spiral liquid pipe 3, the flow rate of the carbon dioxide generated by the microorganisms in the culture solution is increased, so that the situation of the microorganisms can be obtained in a timely and real-time manner.

[0030] A control assembly 8 for controlling the rotation of the spiral liquid pipe 3 and a bracket 9 for supporting the positive and negative pressure pipe 7 are arranged on the base 2. The bracket 9 plays a role in supporting the positive and negative pressure pipe 7 and supplementing gas into the positive and negative pressure pipe 7.

[0031] Specifically, the control assembly 8 includes a driven pulley 81, a driving pulley 82, a transmission belt 83, a transmission shaft 84 and a driving motor 85. The driven pulley 81 is fixedly sleeved outside one of the connecting pipes 4. The transmission shaft 84 is rotatably connected to one of the support plates 21, and the driving pulley 82 is fixedly sleeved thereon. The driving pulley 82 is connected to the driven pulley 81 through the transmission belt 83. The driving motor 85 is fixedly arranged on the top of the base 2, and its output shaft is fixedly connected to one end of the transmission shaft 84. The driving motor 85 provides driving force for the rotation of the transmission shaft 84. Through the transmission of the transmission belt 83, the connecting pipe 4 drives the spiral liquid pipe 3 to rotate. When the spiral liquid pipe 3 rotates, the culture solution near the bottom inside it flows.

[0032] The bracket 9 includes a cylinder 91, a connecting frame 92, a control motor 93, a transmission gear 94 and a transmission rack 95. One end of the cylinder liner 911 of the cylinder 91 is hinged to one side of the base 2. The free end of the piston rod 912 of the cylinder 91 is rotatably connected to the horizontal pipe 71 through the connecting frame 92. The control motor 93 is fixedly arranged on one side of the cylinder liner 911, and its output shaft is fixedly connected to the transmission gear 94. The transmission rack 95 meshes with the transmission gear 94, and one end of it is fixedly connected to the outer wall of the piston rod 912. The control motor 93 controls the telescopic movement of the cylinder 91 through the meshing cooperation of the transmission gear 94 and the transmission rack 95. A short connecting pipe 9111 communicating with the inner cavity is fixedly connected to the outer peripheral wall of the cylinder liner 911. When the cylinder 91 expands and contracts, the short connecting pipe 9111 will intake and exhaust gas. The short connecting pipe 9111 is communicated with the intake pipe 7112 through a pipeline. When the short connecting pipe 9111 exhausts gas, the matching pipe valve 5 can perform a pressure flushing on the spiral liquid pipe 3. Embodiment

[0033] Please refer to Figure 2 The difference from Embodiment 1 is that the transmission shaft 84 is rotatably connected to both support plates 21. A positioning block 101 is screwed on the transmission shaft 84. The top of the positioning block 101 is fixedly connected with an arc-shaped heating plate 102 located below the spiral liquid pipe 3. The spiral liquid pipe 3 is made of a material with good thermal conductivity. The inner arc wall of the arc-shaped heating plate 102 is provided with electric heating tubes with adjustable heating temperature. The electric heating tubes can provide the culture temperature for the culture solution in the spiral liquid pipe 3. A guide shaft 211 slidably connected to the positioning block 101 is fixedly connected between the two support plates 21. When the heating temperature is changed, the temperature of the culture solution in the spiral liquid pipe 3 is also changed. Moreover, since the arc-shaped heating plate 102 will move synchronously with the culture solution when the spiral liquid pipe 3 rotates, the flowing culture solution can be continuously heated evenly. This setting has the function of quickly changing the culture temperature of the culture solution, which is convenient for accurately detecting the activities of microorganisms at different culture temperatures.

[0034] Furthermore, both sides of the positioning block 101 are fixedly connected with heat dissipation fans 103 located on both sides of the arc-shaped heating plate 102. The function of the heat dissipation fans 103 is to cool the spiral liquid pipe 3 as needed. That is to say, when the culture solution in the spiral liquid pipe 3 needs to be quickly cooled, air-cooling can be achieved by starting the heat dissipation fans 103.

[0035] Working principle: I. Vacuum pumping treatment: Control the sliding of the plunger 52 so that the adapter pipe 611 and the positive and negative pressure pipes 7 are both connected to the connecting pipe 4. Then start the external vacuum pump. The positive and negative pressure pipes 7 exhaust air outward. The plug disk 62 will move towards the direction of the adapter pipe 611 under the action of suction force and block the adapter pipe 611 at the same time, and then the vacuum pumping treatment is completed.

[0036] Inject the culture solution containing microorganisms: Control the sliding of the plunger 52 so that the adapter pipe 611 and the positive and negative pressure pipes 7 are both in a blocked state. Then use a syringe to inject the culture solution containing microorganisms into the raw material turnover cavity in one of the sterile turnover cylinders 6. Due to the injection of the culture solution, the plug disk 62 will move outward. After the injection is completed, control the sliding of the plunger 52 on the pipe valve 5 corresponding to the sterile turnover cylinder 6 so that the channel 521 and the adapter pipe 611 are connected. The positive and negative pressure pipes 7 are blocked by the plunger 52 and are not connected to the valve pipe 51. At this time, the spiral liquid pipe 3 will suck the culture solution in the above-mentioned sterile turnover cylinder 6 due to the negative pressure. Then control the sliding of the plunger 52 on the pipe valve 5 corresponding to the sterile turnover cylinder 6 so that the adapter pipe 611 on the sterile turnover cylinder 6 is blocked, and the positive and negative pressure pipes 7 are connected to the valve pipe 51. At this time, the injection of the culture solution is completed.

[0037] Inject oxygen: Inject oxygen into the other sterile turnover cylinder 6 with a syringe in the above-mentioned manner. After the oxygen injection, control both ends of the positive and negative pressure pipes 7 to be in a state of being connected to the spiral liquid pipe 3 through the pipe valve 5. At this time, the positive and negative pressure pipes 7 and the spiral liquid pipe 3 are in a closed-loop connection state.

[0038] Detection: Start the drive motor 85, the transmission shaft 84 rotates. Through the transmission of the transmission belt 83, the spiral liquid pipe 3 rotates. When the spiral liquid pipe 3 rotates, the culture solution inside it will flow. When the culture solution flows, airflows will be formed at both ends of the culture solution in the spiral liquid pipe 3. The CO2 detector 1 in the positive and negative pressure pipes 7 can detect the carbon dioxide generated by the activities of microorganisms in a timely and real-time manner.

[0039] Post-detection cleaning: Open the control valve 5, inject cleaning liquid into the spiral liquid pipe 3 through the sterile turnover cylinder 6, then close the control valve 5, start the control motor 93, the driving gear 94 rotates to drive the driving rack 95 to move, and then drive the cylinder 91 to contract. At this time, the gas generated by the contraction of the cylinder 91 will enter the positive and negative pressure pipe 7, the air pressure in the positive and negative pressure pipe 7 increases, and then quickly open the control valve 5. The cleaning liquid mixture in the spiral liquid pipe 3 will push open the plug disk 62 and be discharged at high speed through the discharge pipe 612. Such a cycle can thoroughly clean the inner cavities of the positive and negative pressure pipe 7 and the spiral liquid pipe 3 without affecting the detection of other microorganisms.

[0040] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and inventive concept of the present invention, makes equivalent substitutions or changes, and should be covered by the protection scope of the present invention.

Claims

1. A CO2 sensor-based real-time microbial detection device, comprising a CO2 detector (1) and a base (2), characterized in that: A horizontally distributed spiral liquid pipe (3) is arranged on the top of the base (2), and connecting pipes (4) coaxial with the spiral axis are arranged at both ends of the spiral liquid pipe (3). A support plate (21) rotatably connected to the connecting pipe (4) is fixedly connected to the top of the base (2), and a pipe valve (5) is connected to one end of the connecting pipe (4). The outer peripheral wall of the pipe valve (5) is fixedly connected to a sterile rotary cylinder (6). The pipe valves (5) located at both ends of the spiral liquid pipe (3) are connected to a positive and negative pressure pipe (7) together. The pipe valve (5) has the function of controlling the sterile rotary cylinder (6) and the positive and negative pressure pipes (7) to be connected to the spiral liquid pipe (3) at the same time, not to be connected at the same time, and to be blocked. The CO2 detector (1) is arranged in the positive and negative pressure pipes (7), and a control assembly (8) for controlling the rotation of the spiral liquid pipe (3) and a bracket (9) for supporting the positive and negative pressure pipes (7) are arranged on the base (2).

2. The real-time microbial detection device based on CO2 sensor according to claim 1 is characterized in that: The aseptic revolving cylinder (6) comprises a cylinder (61) and a plug disc (62); one end of the cylinder (61) is fixedly connected to a transfer tube (611) connected to the pipe valve (5) and the other end is open; the plug disc (62) is sleeved in the cylinder (61) and is in a sealed sliding fit; a rubber plug (621) is provided through the middle of the plug disc (62).

3. The real-time microbial detection device based on CO2 sensor according to claim 2 is characterized in that: The pipe valve (5) comprises a valve pipe (51), a plunger (52) sleeved in the valve pipe (51), and an electric push rod (54) for controlling the sliding of the plunger (52); one end of the valve pipe (51) is sleeved in the connecting pipe (4) and is connected in a rotating sealed manner; the outer peripheral wall of the valve pipe (51) is fixedly connected to one end of the positive and negative pressure pipes (7), and the inner cavities of the two are in communication; one end of the transfer pipe (611) is connected to a position of the outer peripheral wall of the valve pipe (51) close to the free end, and the inner cavities of the two are in communication; one end of the plunger (52) is provided with a channel (521); the outer peripheral wall of the plunger (52) is provided with a hole 1 (522) and a hole 2 (523) which are axially distributed and both are in communication with the channel (521); and the electric push rod (54) is fixedly arranged on one side of the valve pipe (51).

4. The real-time microbial detection device based on CO2 sensor according to claim 3 is characterized in that: The positive and negative pressure pipes (7) are gate-shaped and a switching tube (711) is serially connected to the horizontal tube (71) thereon; one end of the switching tube (711) is fixedly connected to an exhaust pipe (7111) and the other end is fixedly connected to an intake pipe (7112); the outer peripheral wall of the cylinder (61) is fixedly connected to a discharge pipe (612) near the mouth.

5. The real-time microbial detection device based on CO2 sensor according to claim 4 is characterized in that: The support (9) comprises a cylinder (91), a connecting frame (92), a control motor (93), a transmission gear (94) and a transmission rack (95); one end of a cylinder sleeve (911) on the cylinder (91) is hingedly connected to one side of the base (2); a free end of a plug shaft (912) on the cylinder (91) is rotatably connected via the connecting frame (92) and a transverse tube (71); the control motor (93) is fixedly arranged on one side of the cylinder sleeve (911) and its output shaft is fixedly connected to the transmission gear (94); the transmission rack (95) is meshed with the transmission gear (94) and one end of the transmission rack (95) is fixedly connected to the outer wall of the plug shaft (912); a short pipe (9111) communicating with the inner cavity is fixedly connected to the outer peripheral wall of the cylinder sleeve (911); the short pipe (9111) is connected to the intake pipe (7112) via a pipeline.

6. The real-time microbial detection device based on CO2 sensor according to claim 1 is characterized in that: The control assembly (8) comprises a passive pulley (81), a driving pulley (82), a transmission belt (83), a transmission shaft (84) and a driving motor (85); the passive pulley (81) is fixedly sleeved on the outside of one of the connecting tubes (4); the transmission shaft (84) is rotatably connected to one of the support plates (21) and a driving pulley (82) is fixedly sleeved thereon; the driving pulley (82) is connected to the passive pulley (81) via a transmission belt (83); and the driving motor (85) is fixedly arranged on the top of the base (2) and an output shaft thereof is fixedly connected to one end of the transmission shaft (84).

7. The CO2 sensor-based real-time microbial detection device according to claim 6, characterized in that: The transmission shaft (84) and the two support plates (21) are both rotatably connected, a positioning block (101) is screwed onto the transmission shaft (84), a top of the positioning block (101) is fixedly connected to an arc-shaped heating plate (102) located below the spiral liquid pipe (3), and a guide shaft (211) slidably connected to the positioning block (101) is fixedly connected between the two support plates (21).

8. The CO2 sensor-based real-time microbial detection device according to claim 7, characterized in that: The two sides of the positioning block (101) are fixedly connected to cooling fans (103) located on the two sides of the arc-shaped heating plate (102).

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