An ATP Immobilized Enzyme Catalytic Synthesis Device and Synthesis Process

Abstract

The present invention relates to the technical field of immobilized enzyme catalysis, and specifically to an ATP immobilized enzyme catalytic synthesis device and synthesis process. The outer wall of the catalytic box is fixedly connected to a reaction liquid placement box, the outer wall of the catalytic box is fixedly connected to a second connecting pipe on a side away from the reaction liquid placement box, and the outer wall of the second connecting pipe is fixedly connected to a second interface. The ATP immobilized enzyme catalytic synthesis device and synthesis process, by forming the reaction box as an inclined box with a slope, prevents the reaction liquid from contacting the enzyme for a long time after entering the box, thereby preventing the enzyme activity from being reduced and the catalytic efficiency from being unable to be improved. When the reaction liquid enters the interior of the reaction box through the second interface, a rotating column is provided on the inner wall of the reaction box, and the rotating column drives rotating blades in a circular array to rotate. The outer wall of the rotating column is provided with a plurality of placement disks, and circular holes are opened on the surfaces of the placement disks. The porous carrier facilitates the diffusion of the reaction liquid to the active site of the enzyme, thereby improving the catalytic efficiency.

Description

Technical Field

[0001] The invention relates to the technical field of immobilized enzyme catalysis, in particular to an ATP immobilized enzyme catalysis synthesis device and a synthesis process. Background Art

[0002] In the pharmaceutical field, immobilized enzymes can improve the efficiency and purity of drug production, reduce costs, and achieve targeted synthesis and refined control of drugs. For example, immobilized penicillin acylase is used to produce various semi-synthetic penicillins and cephalosporins. In the food industry, immobilized enzymes can be used to improve food quality, such as using immobilized tannase and β-glucosidase to remove turbidity and enhance the flavor of tea beverages. In the energy field, immobilized enzymes can be used in the production of biofuels, improving conversion efficiency and stability and promoting the development of sustainable energy. In environmental governance, immobilized enzymes can be used for wastewater treatment and pollutant degradation, reducing the harm of wastewater to the environment.

[0003] In existing immobilized enzyme processes, the reaction liquid, after entering the chamber, comes into contact with the enzyme for a long time, which reduces the enzyme activity and thus fails to improve the catalytic efficiency. Furthermore, the catalytic efficiency of the immobilized enzyme is related to the properties of the carrier. Different carrier materials, carrier particle sizes, and surface areas will affect the degree of contact between the substrate and the enzyme. Therefore, we propose an ATP immobilized enzyme catalytic synthesis device and synthesis process. Summary of the Invention

[0004] The present invention provides the following technical solution: an ATP immobilized enzyme catalytic synthesis device, comprising a catalyst box, the outer wall of the catalyst box is fixedly connected to a reaction liquid placement box, the outer wall of the catalyst box is fixedly connected to a second connecting pipe on a side away from the reaction liquid placement box, the outer wall of the second connecting pipe is fixedly connected to a second interface, the outer wall of the catalyst box is provided with a high-concentration liquid box on a side away from the reaction liquid placement box, the outer wall of the high-concentration liquid box is fixedly connected to a first connecting pipe, the outer wall of the first connecting pipe is fixedly connected to a first interface, the inner wall of the catalyst box is provided with a reaction box, and the inner wall of the reaction box is provided with a placement component;

[0005] The placement assembly includes a fixed block located on the inner wall of the reaction box, a driving motor is provided on the outer wall of the fixed block, a rotating column is provided at the other end of the outer wall of the fixed block, a rotating blade is provided on the outer wall of the rotating column, a connecting long rod is provided on the outer wall of the connecting long rod, and a plurality of placement plates are provided on the outer wall of the connecting long rod, and the plurality of placement plates are distributed in a linear array on the outer wall of the connecting long rod, and the outer walls of the plurality of placement plates are provided with circular holes;

[0006] The inner wall of the reaction box is provided with a button, and the button and the rotating blade are located on the same horizontal line. The inner wall of the reaction box is provided with a first sliding groove, and the outer wall of the first sliding groove is slidably connected to a first sliding plate. The inner wall of the reaction box is provided with a second sliding groove on a side close to the first sliding groove, and the outer wall of the second sliding groove is slidably connected to a second sliding plate.

[0007] As a preferred technical solution of the present invention, the first sliding plate and the second sliding plate are respectively located at the left and right ends of the inner wall of the reaction box, and the first sliding plate and the second sliding plate slide in opposite directions.

[0008] As a preferred technical solution of the present invention, the outer wall of the reaction box is provided with a movable component, and the movable component includes an active cavity located on the outer wall of the reaction box, the inner wall of the active cavity is provided with a piston, the outer wall of the piston is fixedly connected to a connecting rod, and the outer wall of the connecting rod is provided with a pointer.

[0009] As a preferred technical solution of the present invention, a monitoring component is provided on the outer wall of the connecting rod, and the monitoring component includes a speed measuring box located on the outer wall of the connecting rod, a pressing rod is provided on the outer wall of the speed measuring box, and a connecting block is fixedly connected to the outer wall of the speed measuring box.

[0010] As a preferred technical solution of the present invention, the driving motor and the monitoring component are electrically connected.

[0011] As a preferred technical solution of the present invention, the outer wall of the pointer fits with the outer wall of the pressing rod.

[0012] As a preferred technical solution of the present invention, the rotation rate of the rotating blade is different from the rotation rate of the connecting long rod.

[0013] As a preferred technical solution of the present invention, an outlet is provided on the outer wall of the catalyst box, a placement groove is provided on the top of the catalyst box, and a T-shaped guide plate is fixedly connected to the inner wall of the reaction box.

[0014] An ATP immobilized enzyme catalytic synthesis process, which includes the following process:

[0015] S1: The reaction liquid enters the inner wall of the reaction box through the second connecting pipe. The reaction box is set as a sloped plate. When the liquid enters the reaction box, it flows from the top to the bottom of the reaction box and then flows out from the outlet;

[0016] S2: When the reaction liquid enters the reaction box, the rotating blades on the inner wall push the liquid to flow, preventing it from settling at the bottom and ensuring that the placement plate connected to the outer wall of the long rod is in full contact with the reaction liquid during rotation;

[0017] S3: A button is provided at the bottom of the rotating blade. When the rotating blade rotates, the blade presses the button, causing the first sliding plate and the second sliding plate to slide in the sliding groove, effectively controlling the flow rate of the reaction liquid when it flows from the top reaction chamber to the bottom reaction chamber;

[0018] S4: There is a movable component on the top of the reaction box. Carbon dioxide will be produced when the reaction liquid and enzyme react. The reaction box is a closed box. When the generated gas pushes the piston to move upward, the pointer connected to the side of the piston presses the pressing rod for real-time monitoring. When the rate is too low, high-concentration reaction liquid in the high-concentration liquid tank on the side is added to accelerate the catalytic reaction of the enzyme. When the rate is too high, the rotation speed of the rotating blade is slowed down.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] 1. The ATP immobilized enzyme catalytic synthesis device and synthesis process are characterized in that the reaction box is an inclined box with a slope, which prevents the reaction liquid from contacting the enzyme for a long time after entering the box, thereby reducing the activity of the enzyme and failing to improve the catalytic efficiency. When the reaction liquid enters the interior of the reaction box through the second interface, a rotating column is provided on the inner wall of the reaction box, which drives the rotating blades in a circular array to rotate. The outer wall of the rotating column is provided with multiple placement trays, and circular holes are opened on the surface of the placement trays. The porous carriers facilitate the reaction liquid to diffuse more easily to the active site of the enzyme, thereby improving the catalytic efficiency. A first sliding plate and a second sliding plate are provided on the inner wall of the reaction box. When the reaction liquid flows from the top reaction chamber into the bottom reaction chamber, the provision of the partition effectively prevents the reaction liquid from splashing due to inertia after flowing from a high place to a low place.

[0021] 2. The ATP immobilized enzyme catalytic synthesis device and synthesis process, by squeezing a button after the rotating blade rotates, since the initial positions of the two sliding plates are located at the left and right ends of the inner wall of the reaction chamber, the first sliding plate and the second sliding plate slide in opposite directions to each other, and the rotation speed of the rotating blade is equal to the movement speed of the first sliding plate and the second sliding plate. The arrangement of the first sliding plate and the second sliding plate effectively controls the flow rate of the falling reaction liquid. When the immobilized enzyme reacts too fast or too slow, it is effectively controlled, thereby improving the accuracy of the equipment. The enzyme will not suffer from low catalytic efficiency due to an excessively fast reaction liquid flow rate during catalysis, nor will it suffer from prolonged contact with the immobilized enzyme due to an excessively slow reaction liquid flow rate, which will reduce the enzyme's activity and lead to a decrease in working efficiency.

[0022] 3. The ATP immobilized enzyme catalytic synthesis device and synthesis process also have a monitoring component provided on the outer wall of the connecting rod. When the piston drives the connecting rod to move, when the pressing rod detects that the pointer moves at a fast speed, the speed measuring box adjusts the drive motor to reduce the rotation speed of the rotating blades, slowing down the flow speed of the reaction liquid in the reaction box, so that the reaction liquid can fully react with the immobilized enzyme for catalytic reaction. When the pointer moves at a slow speed, the high-concentration liquid box on the side wall is opened, allowing the high-concentration reaction liquid in the high-concentration liquid box to enter the reaction box for full combination, thereby accelerating the reaction rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a structural schematic diagram of an ATP immobilized enzyme catalytic synthesis device and synthesis process;

[0024] Figure 2 This is the second structural diagram of an ATP immobilized enzyme catalytic synthesis device and synthesis process;

[0025] Figure 3 This is a schematic diagram of the reaction box structure of an ATP immobilized enzyme catalytic synthesis device and synthesis process;

[0026] Figure 4 A schematic cross-sectional view of the reaction chamber structure of an ATP immobilized enzyme catalytic synthesis device and synthesis process;

[0027] Figure 5 A schematic diagram of the structure of components for an ATP immobilized enzyme-catalyzed synthesis device and synthesis process;

[0028] Figure 6 The second schematic cross-sectional diagram of the structure of the components of an ATP immobilized enzyme catalytic synthesis device and synthesis process;

[0029] Figure 7 A schematic cross-sectional view of the reaction chamber structure of an ATP immobilized enzyme catalytic synthesis device and synthesis process;

[0030] Figure 8 Schematic diagram of the structure of active components of an ATP immobilized enzyme-catalyzed synthesis device and synthesis process.

[0031] In the figure: 1. Catalytic box; 2. Placement tank; 3. Outlet; 4. Reaction liquid placement box; 5. High-concentration liquid box; 6. First connecting pipe; 7. First interface; 8. Second interface; 9. Second connecting pipe; 10. Drive motor; 11. Connecting block; 12. Monitoring component; 121. Speed measuring box; 122. Pressing rod; 13. Movable component; 131. Movable chamber; 132. Piston; 133. Connecting rod; 134. Pointer; 14. Reaction box; 15. Placement component; 151. Fixed block; 152. Rotating column; 153. Rotating blade; 154. Connecting long rod; 155. Placement plate; 156. Round hole; 16. Button; 17. First sliding groove; 18. First sliding plate; 19. T-shaped guide plate; 20. Second sliding groove; 21. Second sliding plate. DETAILED DESCRIPTION

[0032] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0033] See also Figure 1-8 An ATP immobilized enzyme catalytic synthesis device includes a catalyst box 1, the outer wall of the catalyst box 1 is fixedly connected to a reaction liquid placement box 4, the outer wall of the catalyst box 1 is fixedly connected to a second connecting pipe 9 on the side away from the reaction liquid placement box 4, the outer wall of the second connecting pipe 9 is fixedly connected to a second interface 8, the outer wall of the catalyst box 1 is provided with a high-concentration liquid box 5 on the side away from the reaction liquid placement box 4, the outer wall of the high-concentration liquid box 5 is fixedly connected to a first connecting pipe 6, the outer wall of the first connecting pipe 6 is fixedly connected to a first interface 7, the inner wall of the catalyst box 1 is provided with a reaction box 14, and the inner wall of the reaction box 14 is provided with a placement component 15.

[0034] The placement assembly 15 includes a fixed block 151 located on the inner wall of the reaction box 14. The outer wall of the fixed block 151 is provided with a drive motor 10. A rotating column 152 is provided at the other end of the outer wall of the fixed block 151. The outer wall of the rotating column 152 is provided with a rotating blade 153. The outer wall of the rotating column 152 is provided with a connecting rod 154. The outer wall of the connecting rod 154 is provided with a plurality of placement plates 155. The plurality of placement plates 155 are distributed in a linear array on the outer wall of the connecting rod 154. The outer walls of the plurality of placement plates 155 are provided with circular holes 156.

[0035] The inner wall of the reaction box 14 is provided with a button 16, which is located on the same horizontal line as the rotating blade 153. The inner wall of the reaction box 14 is provided with a first sliding groove 17, and the outer wall of the first sliding groove 17 is slidably connected to a first sliding plate 18. The inner wall of the reaction box 14 is provided with a second sliding groove 20 on the side close to the first sliding groove 17, and the outer wall of the second sliding groove 20 is slidably connected to a second sliding plate 21.

[0036] It should be noted that the reaction box 14 is an inclined box with a slope, which prevents the reaction liquid from coming into contact with the enzyme for a long time after entering the box, thereby reducing the activity of the enzyme and failing to improve the catalytic efficiency. When the reaction liquid enters the reaction box 14 through the second interface 8, a rotating column 152 is provided on the inner wall of the reaction box 14. The rotating column 152 drives the rotating blades 153 in a circular array to rotate. The outer wall of the rotating column 152 is provided with a plurality of placement trays 155, and circular holes 156 are provided on the surface of the placement trays 155. The porous carrier makes it easier for the reaction liquid to diffuse to the active site of the enzyme, thereby improving the catalytic efficiency. A first sliding plate 18 and a second sliding plate 21 are provided on the inner wall of the reaction box 14. When the reaction liquid flows from the top reaction chamber into the bottom reaction chamber, the setting of the partition effectively avoids the splashing of the reaction liquid due to inertia after the reaction liquid flows from high to low.

[0037] See also Figure 7 The first sliding plate 18 and the second sliding plate 21 are respectively located at the left and right ends of the inner wall of the reaction box 14 , and the first sliding plate 18 and the second sliding plate 21 slide in opposite directions.

[0038] It should be noted that when the rotating blade 153 rotates and the button 16 is squeezed, since the initial positions of the two sliding plates are located at the left and right ends of the inner wall of the reaction box 14, the first sliding plate 18 and the second sliding plate 21 slide in opposite directions to each other, and the rotation speed of the rotating blade 153 is equal to the movement speed of the first sliding plate 18 and the second sliding plate 21. The setting of the first sliding plate 18 and the second sliding plate 21 effectively controls the flow rate of the falling reaction liquid, and is effectively controlled when the immobilized enzyme reacts too fast or too slow, thereby improving the accuracy of the equipment, so that the enzyme will not suffer from low catalytic efficiency due to the excessively fast flow rate of the reaction liquid during catalysis, nor will it suffer from long-term contact with the immobilized enzyme due to the excessively slow flow rate of the reaction liquid, which will reduce the activity of the enzyme and lead to a decrease in working efficiency.

[0039] See also Figure 8 : The outer wall of the reaction box 14 is provided with a movable component 13, the movable component 13 includes an active cavity 131 located on the outer wall of the reaction box 14, the inner wall of the active cavity 131 is provided with a piston 132, the outer wall of the piston 132 is fixedly connected to a connecting rod 133, and the outer wall of the connecting rod 133 is provided with a pointer 134.

[0040] It should be noted that when the immobilized enzyme produces carbon dioxide during catalysis, since the reaction box 14 is a closed box, when the enzyme reaction is too fast, the piston 132 in the active cavity 131 moves upward rapidly. Since the outer wall of the piston 132 is fixedly connected to the connecting rod 133, the outer wall of the connecting rod 133 is provided with a pointer 134. When the pointer moves, it drives the monitoring component 12 connected to the outer wall to monitor the catalytic reaction rate of the inner cavity. When the enzyme reaction is too slow, the piston 132 moves slowly in the inner cavity.

[0041] See also Figure 8 : A monitoring assembly 12 is provided on the outer wall of the connecting rod 133, and the monitoring assembly 12 includes a speed measuring box 121 located on the outer wall of the connecting rod 133. A pressing rod 122 is provided on the outer wall of the speed measuring box 121, and a connecting block 11 is fixedly connected to the outer wall of the speed measuring box 121.

[0042] It should be noted that a monitoring component 12 is provided on the outer wall of the connecting rod 133. When the piston drives the connecting rod 133 to move, when the pressing rod 122 detects that the pointer 134 moves faster, the speed measuring box 121 adjusts the driving motor 10, reduces the rotation rate of the rotating blade 153, and slows down the flow rate of the reaction liquid in the reaction box, so that the reaction liquid can fully catalyze the reaction with the immobilized enzyme. When it is detected that the pointer 134 moves slowly, the high-concentration liquid box 5 on the side wall is opened, so that the high-concentration reaction liquid in the high-concentration liquid box 5 enters the reaction box for full combination, thereby accelerating the reaction rate.

[0043] See also Figure 3 : The driving motor 10 and the monitoring component 12 are electrically connected.

[0044] It should be noted that due to the electrical connection between the drive motor 10 and the monitoring component 12, the device effectively controls the reaction rate of the enzyme, preventing the enzyme from having low catalytic efficiency due to the excessively fast flow rate of the reaction liquid during catalysis, and also preventing the enzyme from being in contact with the immobilized enzyme for a long time due to the excessively slow flow rate of the reaction liquid, thereby reducing the activity of the enzyme and leading to a decrease in working efficiency.

[0045] See also Figure 8 : The outer wall of the pointer 134 fits with the pressing rod 122.

[0046] It should be noted that when the pointer 134 moves, the pressing rod 122 is pressed to effectively monitor the moving speed of the piston 132 and the reaction speed in the reaction box.

[0047] See also Figure 5 : The rotation rate of the rotating blade 153 is different from the rotation rate of the connecting long rod 154.

[0048] It should be noted that the rotation rate of the connecting long rod 154 is always maintained at a uniform speed. When the piston drives the pointer 134 to move too fast, the monitoring component 12 is electrically connected to the drive motor 10, which effectively reduces the rotation speed of the rotating blade 153. When the rotating blade 153 with reduced speed is rotating, pressing the bottom button 16 causes the sliding plate to slide, thereby reducing the flow rate of the reaction liquid in the reaction box.

[0049] See also Figure 1 An outlet 3 is provided on the outer wall of the catalyst box 1 , a placement slot 2 is provided on the top of the catalyst box 1 , and a T-shaped guide plate 19 is fixedly connected to the inner wall of the reaction box 14 .

[0050] It should be noted that when the reaction liquid after the enzyme reaction flows out from the outlet 3, a T-shaped guide plate 19 is fixedly connected to the inner wall of the reaction box 14 to reduce splashing caused by inertia when the reaction liquid falls through the sliding plate and flows to a lower place.

[0051] An ATP immobilized enzyme catalytic synthesis process includes the following processes:

[0052] S1: The reaction liquid enters the inner wall of the reaction box 14 through the second connecting pipe 9. The reaction box 14 is configured as a sloped plate. After the liquid enters the reaction box 14, it flows from the top to the bottom of the reaction box 14 and then flows out from the outlet 3.

[0053] S2: When the reaction liquid enters the reaction box 14, the rotating blades 153 on the inner wall push the liquid to flow, preventing it from settling and accumulating on the bottom, ensuring that the placement plate 155 on the outer wall of the connecting long rod 154 is in full contact with the reaction liquid during rotation;

[0054] S3: A button 16 is provided at the bottom of the rotating blade 153. When the rotating blade 153 rotates, the blade presses the button 16, causing the first sliding plate 18 and the second sliding plate 21 to slide in the sliding groove, effectively controlling the flow rate of the reaction liquid when it flows from the top reaction chamber to the bottom reaction chamber;

[0055] S4: A movable component 13 is provided on the top of the reaction box 14. Carbon dioxide is generated when the reaction liquid and the enzyme react. The reaction box 14 is a closed box. When the generated gas pushes the piston 132 to move upward, the pointer 134 connected to the side of the piston 132 presses the pressing rod 122 for real-time monitoring. When the rate is too low, the high-concentration reaction liquid in the high-concentration liquid tank 5 on the side is added to accelerate the catalytic reaction of the enzyme. When the rate is too high, the rotation speed of the rotating blade 153 is slowed down.

[0056] Working principle: when the device needs to be used, after the reaction liquid enters the reaction box 14 through the second interface 8, a rotating column 152 is provided on the inner wall of the reaction box 14. The rotating column 152 drives the rotating blades 153 in a circular array to rotate. The outer wall of the rotating column 152 is provided with a plurality of placement plates 155. Circular holes 156 are opened on the surface of the placement plates 155. The porous carrier makes it easier for the reaction liquid to diffuse to the active site of the enzyme, thereby improving the catalytic efficiency. A first sliding plate 18 and a second sliding plate 21 are provided on the inner wall of the reaction box 14. When the reaction liquid flows from the top reaction chamber into the bottom reaction chamber, the setting of the partition effectively avoids the splashing of the reaction liquid due to the inertial force after the reaction liquid flows from a high place to a low place.

[0057] When the rotating blade 153 rotates and the button 16 is squeezed, since the initial positions of the two sliding plates are located at the left and right ends of the inner wall of the reaction box 14, the first sliding plate 18 and the second sliding plate 21 slide in opposite directions to each other, and the speed of rotation of the rotating blade 153 is equal to the speed of movement of the first sliding plate 18 and the second sliding plate 21. The arrangement of the first sliding plate 18 and the second sliding plate 21 effectively controls the flow rate of the falling reaction liquid. Since the reaction box 14 is a closed box, a monitoring component 12 is provided on the outer wall of the connecting rod 133. When the piston drives the connecting rod 1 After the movement of the pointer 134, since the rotation rate of the connecting rod 154 is always kept at a constant speed, when the pressing rod 122 detects that the pointer 134 moves faster, the speed measuring box 121 adjusts the driving motor 10 to reduce the rotation rate of the rotating blade 153, slowing down the flow rate of the reaction liquid in the reaction box, so that the reaction liquid can fully react with the immobilized enzyme. When it is detected that the pointer 134 moves slower, the high-concentration liquid box 5 on the side wall is opened to allow the high-concentration reaction liquid in the high-concentration liquid box 5 to enter the reaction box for full combination, thereby accelerating the reaction rate.

[0058] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. An ATP immobilized enzyme catalytic synthesis device, comprising a catalyst box (1), characterized in that: The outer wall of the catalytic box (1) is fixedly connected to a reaction liquid placement box (4), the outer wall of the catalytic box (1) is fixedly connected to a second connecting pipe (9) on a side away from the reaction liquid placement box (4), the outer wall of the second connecting pipe (9) is fixedly connected to a second interface (8), the outer wall of the catalytic box (1) is provided with a high-concentration liquid box (5) on a side away from the reaction liquid placement box (4), the outer wall of the high-concentration liquid box (5) is fixedly connected to a first connecting pipe (6), the outer wall of the first connecting pipe (6) is fixedly connected to a first interface (7), the inner wall of the catalytic box (1) is provided with a reaction box (14), the reaction box (14) is an inclined box with a slope, and the inner wall of the reaction box (14) is provided with a placement component (15); The placement assembly (15) includes a fixed block (151) located on the inner wall of the reaction box (14), the outer wall of the fixed block (151) is provided with a driving motor (10), the other end of the outer wall of the fixed block (151) is provided with a rotating column (152), the outer wall of the rotating column (152) is provided with a rotating blade (153), the outer wall of the rotating column (152) is provided with a connecting rod (154), the outer wall of the connecting rod (154) is provided with a plurality of placement plates (155), the plurality of placement plates (155) are distributed in a linear array on the outer wall of the connecting rod (154), and the outer walls of the plurality of placement plates (155) are provided with circular holes (156); The inner wall of the reaction box (14) is provided with a button (16), and the button (16) and the rotating blade (153) are located on the same horizontal line. The inner wall of the reaction box (14) is provided with a first sliding groove (17), and the outer wall of the first sliding groove (17) is slidably connected to a first sliding plate (18). The inner wall of the reaction box (14) is provided with a second sliding groove (20) on a side close to the first sliding groove (17), and the outer wall of the second sliding groove (20) is slidably connected to a second sliding plate (21).

2. An ATP immobilized enzyme catalytic synthesis device according to claim 1, characterized in that: The first sliding plate (18) and the second sliding plate (21) are respectively located at the left and right ends of the inner wall of the reaction box (14), and the first sliding plate (18) and the second sliding plate (21) slide in opposite directions.

3. An ATP immobilized enzyme catalytic synthesis device according to claim 1, characterized in that: The outer wall of the reaction box (14) is provided with a movable assembly (13), and the movable assembly (13) includes an movable cavity (131) located on the outer wall of the reaction box (14). The inner wall of the movable cavity (131) is provided with a piston (132). The outer wall of the piston (132) is fixedly connected to a connecting rod (133), and the outer wall of the connecting rod (133) is provided with a pointer (134).

4. An ATP immobilized enzyme catalytic synthesis device according to claim 3, characterized in that: The outer wall of the connecting rod (133) is provided with a monitoring assembly (12), and the monitoring assembly (12) includes a speed measuring box (121) located on the outer wall of the connecting rod (133). The outer wall of the speed measuring box (121) is provided with a pressing rod (122), and the outer wall of the speed measuring box (121) is fixedly connected to a connecting block (11).

5. An ATP immobilized enzyme catalytic synthesis device according to claim 4, characterized in that: The driving motor (10) is electrically connected to the monitoring component (12).

6. An ATP immobilized enzyme catalytic synthesis device according to claim 3, characterized in that: The outer wall of the pointer (134) fits into the outer wall of the pressing rod (122).

7. The ATP immobilized enzyme catalytic synthesis device according to claim 1, characterized in that: The rotation speed of the rotating blade (153) is different from the rotation speed of the connecting rod (154).

8. The ATP immobilized enzyme catalytic synthesis device according to claim 1, characterized in that: An outlet (3) is provided on the outer wall of the catalytic box (1), a placement groove (2) is provided on the top of the catalytic box (1), and a T-shaped guide plate (19) is fixedly connected to the inner wall of the reaction box (14).

9. An ATP immobilized enzyme catalytic synthesis process, applied to an ATP immobilized enzyme catalytic synthesis device according to any one of claims 1 to 8, characterized in that: Including the following processes: S1: The reaction liquid enters the inner wall of the reaction box (14) through the second connecting pipe (9). After the liquid enters the reaction box (14), it flows from the top to the bottom of the reaction box (14) and then flows out from the outlet (3); S2: When the reaction liquid enters the reaction box (14), the rotating blades (153) provided on the inner wall thereof push the liquid to flow, thereby preventing the liquid from settling to the bottom and ensuring that the placement plate (155) provided on the outer wall of the connecting long rod (154) is in full contact with the reaction liquid during rotation; S3: A button (16) is provided at the bottom of the rotating blade (153). When the rotating blade (153) rotates, the blade presses the button (16), causing the first sliding plate (18) and the second sliding plate (21) to slide in the sliding groove, effectively controlling the flow rate of the reaction liquid when it flows from the top reaction chamber to the bottom reaction chamber; S4: A movable component (13) is provided on the top of the reaction box (14). Carbon dioxide is generated when the reaction liquid reacts with the enzyme. The reaction box (14) is a closed box. When the generated gas pushes the piston (132) to move upward, the pointer (134) connected to the side of the piston (132) presses the pressing rod (122) for real-time monitoring. When the rate is too low, the high-concentration reaction liquid in the high-concentration liquid box (5) on the side is added to accelerate the catalytic reaction of the enzyme. When the rate is too high, the rotation speed of the rotating blade (153) is slowed down.

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