Biological aerosol continuous sampling device and method

Abstract

The invention relates to the field of medical devices, in particular to a biological aerosol continuous sampling device and method.The biological aerosol continuous sampling device comprises a sampling assembly, a liquid supplementing assembly and a control assembly, and the sampling assembly comprises a sampling cup, a draught fan located above the sampling cup and a sampling head; the liquid supplementing assembly comprises a liquid storage bottle and a peristaltic pump; the control assembly comprises a liquid shortage sensor and a liquid level sensor, and the peristaltic pump and the fan are controlled to start and stop according to signals sent by the liquid shortage sensor and the liquid level sensor, so that the sampling process is normal; the method is used for solving missing detection of existing biological aerosol collection. The acquisition device cannot continuously sample for a long time; remote monitoring and real-time control of the sampling device cannot be realized, and the collection efficiency is low. The sample collection accuracy is improved through the sampling assembly; missing detection is avoided; the effect of continuous sampling is achieved through the liquid supplementing assembly; combined automatic control over the sampling assembly and the liquid supplementing assembly is achieved through the control assembly, so that the sampling efficiency is improved.

Description

Technical Field

[0001] The present invention relates to the field of medical devices, and more specifically, to a continuous bioaerosol sampling device and method. Background Art

[0002] Collecting bioaerosols in the air and detecting them is an important means for monitoring the transmission of pathogenic microorganisms in the air and bioaerosols. To avoid missing the collection of bioaerosols due to changes in bioaerosols in places such as hospitals, biopharmaceutical workshops, and laboratories, it is necessary to continuously sample the aerosol for a long time.

[0003] Currently, bioaerosol collection devices usually have difficulty in achieving long-term automatic sampling. The main reasons are as follows: After the aerosol collected by the filter membrane of the filtration sampling device is saturated, the collection efficiency will be greatly reduced, and the filter membrane must be replaced when detecting the collected sample, and it can only be carried out after elution treatment; due to the uncertainty of liquid volatilization in the conventional wet sampling device, it is difficult to perform long-term automatic sampling, and it is even more impossible to monitor and control the real-time state of the device.

[0004] The problems of existing bioaerosol collection, such as missed detection; the collection device cannot continuously sample for a long time and needs to be sampled intermittently after replenishing the liquid; and the remote monitoring and real-time control of the sampling device cannot be achieved, and the sampling liquid cannot be automatically replenished, resulting in low collection efficiency of current bioaerosols, are urgently needed to be solved in this field. Summary of the Invention

[0005] The present invention aims to overcome at least one defect (insufficiency) of the above-mentioned prior art, and provides a continuous bioaerosol sampling device and method, which are used to solve the problems of existing bioaerosol collection, such as missed detection; the collection device cannot continuously sample for a long time and needs to be sampled intermittently after replenishing the liquid; and the remote monitoring and real-time control of the sampling device cannot be achieved, and the sampling liquid cannot be automatically replenished, resulting in low collection efficiency of current bioaerosols.

[0006] The technical solution adopted by the present invention is to provide a continuous bioaerosol sampling device, which includes a sampling component, a liquid replenishing component, and a control component. The sampling component includes a sampling cup, a blower located above it, and a sampling head. The sampling cup is used to hold the sampling liquid. The blower is used to generate an airflow rotating along the central axis direction of the sampling cup and cause the sampling liquid to react with the particulate matter in the airflow to form an aerosol. The sampling head is used to collect the aerosol sample. The liquid replenishing component includes a liquid storage bottle and a peristaltic pump. The liquid storage bottle is used to store the sampling liquid. The peristaltic pump is used to continuously replenish the sampling liquid into the sampling cup. The control component includes a liquid shortage sensor and a liquid level sensor. The liquid shortage sensor is arranged between the liquid storage bottle and the peristaltic pump. The liquid level sensor is arranged on the side wall of the sampling cup. The peristaltic pump and the blower control the start and stop according to the signals sent by the liquid shortage sensor and the liquid level sensor to ensure the normal sampling process.

[0007] It is beneficial to hold a quantitative sampling liquid in the sampling cup for reacting with the particulate matter in the air to be measured. The blower inhales the air to be measured into the device and forms a cyclone above the sampling cup, so as to separate the dust-containing particles in the air and react with the sampling liquid, and make the combined solution form an aerosol, thereby improving the accuracy of sample collection. The sampling head samples the high-concentration air microorganisms to avoid the problem of missed detection in sampling. The liquid replenishing component realizes automatic quantitative liquid taking after each sampling, so as to achieve the effect of continuous and uninterrupted sampling. The control component realizes the combined automatic control of the sampling component and the liquid replenishing component to improve the sampling efficiency.

[0008] Further, the liquid replenishing component further includes an infusion tube. A liquid replenishing port is provided at the upper edge of the sampling cup. The infusion tube runs through and connects the liquid storage bottle, the peristaltic pump, and the liquid replenishing port. Detection components are provided at both ends of the infusion tube where it is connected to the liquid replenishing port and the liquid storage bottle respectively.

[0009] It is beneficial to transport the sampling liquid in the liquid storage bottle to the sampling cup through the infusion tube. The liquid shortage sensor arranged in the infusion tube helps to quickly judge whether the liquid storage bottle is in a liquid shortage state. The detection components at both ends of the infusion tube can quickly check the cause of the failure in the liquid replenishing component to improve the use efficiency of the sampling device.

[0010] Further, the blower includes an air inlet and an air outlet arranged diagonally. The air inlet is arranged at the lower edge of the blower, corresponding to the position of the liquid replenishing port. The air outlet is arranged at the upper edge of the blower, corresponding to the position of the sampling head.

[0011] It is beneficial to avoid the situation where the particulate matter to be measured cannot fully react and combine with the sampling liquid through the diagonally distributed air inlets and outlets, which may affect the accuracy of aerosol collection. By having the liquid replenishment port and the air inlet corresponding vertically, when the sampling liquid is injected into the sampling cup, it can immediately react with the particulate matter that has been separated and adhered to the wall of the sampling cup, thereby shortening the sampling time. By having the sampling head and the air outlet corresponding vertically, after the particulate matter and the sampling liquid react before sampling, there is sufficient time for a high-concentration aerosol to form at the sampling head, so as to improve the sampling accuracy.

[0012] Further, the control component further includes a control circuit board and a fault indicator light. The fault indicator light is used to indicate that the sampling liquid in the liquid storage bottle is insufficient and that the sampling liquid in the sampling cup for replenishment is full. The control circuit board is connected to the peristaltic pump, the fan, the fault indicator light, the liquid shortage sensor, and the liquid level sensor, and is used to compare whether each signal coexists and analyze and process the sending order of each signal to control the normal operation of the sampling device.

[0013] It is beneficial to achieve automatic control of each component through the control circuit board, and to give a warning through the fault indicator light when the sampling liquid in the liquid storage bottle is insufficient and when the sampling liquid in the sampling cup does not meet the quantitative value, so as to ensure that the sampling device can perform continuous and uninterrupted sampling and improve the use efficiency of the device.

[0014] Further, it further includes a wireless transmission module and a remote monitoring system. The wireless transmission module is arranged beside the control circuit board and is used to transmit wireless signals to the remote monitoring system to realize the real-time transmission of the working state data of the sampling device to the remote monitoring system.

[0015] It is beneficial to transmit the signals on the control circuit board to the remote monitoring system through the wireless transmission module by wireless transmission, realizing remote real-time control of the sampling device, thereby improving the flexibility of the sampling device in scene application.

[0016] It also provides a method for continuous sampling of bioaerosols, which includes the sampling device described above:

[0017] S1. After connecting to the power supply, the fan sucks the gas in the area to be sampled from the air inlet located on the side wall of the sampling cup;

[0018] S2. The gas sucked between the fan and the sampling cup, under the action of the fan, the dust-containing part in the gas is thrown towards the wall of the sampling cup during rotation;

[0019] S3. After the fan operates for M seconds, the sampling cup starts to inject a quantitative sampling liquid, and the sampling liquid reacts and combines with the dust-containing particles after contacting the wall surface;

[0020] S4. The fan continues to operate and gradually forms a rotating air column, causing the sampling liquid after reacting and combining with dust particles to form an aerosol above the sampling cup.

[0021] S5. The sampling head located above the sampling cup stays in the aerosol for sampling.

[0022] S6. The sampling head that has completed sampling is replaced. The gas that has completed sampling is discharged from the air outlet above the fan, and the sampling liquid that has completed the reaction is discharged through the liquid outlet at the bottom of the sampling cup.

[0023] It is beneficial to first inhale the air to be measured into the device through the above steps, then react with the sampling liquid and collect the aerosol after the reaction, avoiding the problem of missed detection in the collection of bioaerosols.

[0024] Further, the step S2 specifically includes:

[0025] S21. The fan accelerates the gas inhaled from the air inlet by using the impeller rotating at high speed to meet the air flow requirement of the air inlet.

[0026] S22. After the sampling head is connected to the sampling cup, the gas inhaled by the fan can flow spirally downward along the wall of the sampling cup to form a cyclone.

[0027] S23. The dust-containing gas generates centrifugal force during rotation, throwing the particles with a density greater than that of the gas towards the wall of the sampling cup, causing them to combine with the sampling liquid, thus completing the sampling.

[0028] It is beneficial to separate the particulate matter to be measured in the air to be measured through the above steps, enabling the sampling liquid to fully react with the particulate matter, thereby improving the sampling accuracy of the aerosol to be measured.

[0029] Further, the step S3 specifically includes:

[0030] S31. Turn on the instrument switch, the fan starts to operate, and the peristaltic pump starts to run for N seconds until the liquid level sensor detects that the infusion tube is filled with the sampling liquid and sends a full liquid signal to the control circuit board; otherwise, the control circuit board sends an alarm signal to the fault indicator light after N seconds, indicating that there is an abnormality in liquid supplementation.

[0031] S32. After receiving the full liquid signal, the control circuit board controls the peristaltic pump to continue running to fill the capacity P (L) of the sampling cup. The t (s) is the time required for the peristaltic pump to completely fill the sampling cup; where The cross-sectional area of the infusion tube is S (m²), and the flow rate of the sampling liquid in the peristaltic pump is V (m / s).

[0032] S33. During the operation of step S32, the low liquid level sensor in the liquid level sensor detects the liquid level of the sampling cup once: if the low liquid level sensor sends a low-level liquid shortage signal to the control circuit board, the control circuit board controls the peristaltic pump to continue running for Δt seconds (Δt<t); if the low liquid level sensor sends a low-level liquid full signal to the control circuit board, the control circuit board controls the peristaltic pump to stop after running for t seconds;

[0033] S34. During the detection process of step S33, the high liquid level sensor in the liquid level sensor performs a secondary detection on the liquid level of the sampling cup; if the high liquid level sensor sends a high-level liquid shortage signal to the control circuit board, the peristaltic pump of step S32 continues to run; if the high liquid level sensor sends a high-level liquid full signal to the control circuit board, the control circuit board controls the peristaltic pump to stop running immediately and sends an alarm signal to the fault indicator light;

[0034] S35. The sampling liquid in the sampling cup is filled with the quantitative value P(L).

[0035] The above steps are conducive to continuous quantitative injection of the sampling liquid in the sampling cup, thereby achieving a long-term, uninterrupted sampling effect of the sampling device.

[0036] Furthermore, the specific steps of troubleshooting the abnormal fluid replenishment fault in step S31 include:

[0037] S311. The fault indicator light is on, indicating that there is no liquid flowing in the infusion tube, and it is determined that the infusion component is faulty;

[0038] S312. The control circuit board confirms whether there is liquid at the place where the infusion tube is connected to the liquid storage bottle. If not, it is determined that the cause of the fault is insufficient sampling liquid in the liquid storage bottle. The staff removes the liquid storage bottle and releases the alarm state, and the fault indicator light turns off; if yes, the liquid storage bottle fault is eliminated, and the next step of fault cause inquiry is performed;

[0039] S313. The control circuit board confirms whether there is liquid in the infusion port of the infusion tube connected to the sampling cup. If so, it is determined that the fault is caused by an abnormality in the lack of liquid sensor. After the sampling liquid is injected into the sampling cup, the staff replaces the lack of liquid sensor, releases the alarm state, and turns off the fault indicator light; if not, the next step is to query the cause of the fault;

[0040] S314. The control circuit board confirms whether the peristaltic pump is working normally. If so, it is determined that the cause of the fault is an abnormality in the liquid shortage sensor. After the sampling liquid in the sampling cup is injected, the staff will replace the liquid shortage sensor, release the alarm state, and turn off the fault indicator light. If not, it is determined that the cause of the fault is an abnormality in the peristaltic pump, the control switch of the control circuit board is disconnected, and the staff will inspect and replace the peristaltic pump.

[0041] It is beneficial to gradually eliminate the fault causes after the fault indicator light is on through the above steps, so as to quickly determine the fault causes and make the maintenance and repair of the sampling device more convenient.

[0042] Furthermore, before starting a detection in the step S33, the specific installation steps of the liquid level sensor include:

[0043] S331. Three holes distributed in a triangular shape are reserved before installing the sampling cup, so as to install a device for detecting the position of the sampling liquid in the sampling cup;

[0044] S332. There are two liquid level sensors, one low liquid level sensor and one high liquid level sensor. After the electrodes of the two sensors are bent for the first time, they are respectively inserted into the left and right holes in the reserved holes;

[0045] S333. The electrodes of the two sensors are bent for the second time at the left and right holes respectively. After the two bending processes of the high liquid level sensor are completed, it is inserted into the middle hole, so that the two sensors expose three electrodes outside the three reserved holes;

[0046] S334. Connect the three electrodes to the corresponding parts of the USB interface of the sampling cup through wires respectively, and put them into the card slot under the hole, and fix them with hot melt adhesive.

[0047] It is beneficial to adaptively adjust the installation of the liquid level sensor on the sampling cup through the above steps, so that while the sampling cup maintains the quantitative detection of the sampling liquid, the cost of the sampling cup can also be reduced.

[0048] Compared with the prior art, the beneficial effects of the present invention are as follows: The particulate matter in the air to be measured is fully reacted and combined with the quantitative sampling liquid through the sampling component, and the combined solution forms an aerosol, thereby improving the accuracy of sample collection; The high-concentration air microorganisms are sampled through the sampling head to avoid missed detection; The automatic quantitative liquid taking after each sampling is realized through the liquid replenishment component, so as to achieve the effect of continuous and uninterrupted sampling; The combined automatic control of the sampling component and the liquid replenishment component is realized through the control component to improve the sampling efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] Figure 1 It is a schematic structural diagram of the sampling device of the present invention.

[0050] Figure 2 It is a schematic installation diagram of the liquid level sensor of the present invention on the sampling cup.

[0051] Figure 3 It is a gas circuit diagram in the sampling cup of the present invention.

[0052] Figure 4This is the liquid path diagram in the sampling cup of the present invention.

[0053] Figure 5 This is the schematic diagram of the connection of the control circuit board of the present invention.

[0054] Explanation of the attached drawing reference numerals: Power supply 12, control circuit board 18, data line 33, USB interface 35, wireless transmission module 16, remote monitoring system 38, sampling head 32, fan 20, liquid storage bottle 4, infusion tube 15, liquid shortage sensor 14, high liquid level sensor 34, low liquid level sensor 36, peristaltic pump 5, sampling cup 32, liquid outlet 37, air inlet 24, air outlet 29, liquid replenishment port 23. Detailed implementation manners

[0055] The attached drawings of the present invention are only for illustrative purposes and should not be construed as a limitation to the present invention. For better illustration of the following embodiments, some components in the drawings may be omitted, enlarged or reduced, which do not represent the dimensions of the actual product; for those skilled in the art, it is understandable that some well-known structures and their descriptions in the drawings may be omitted. Embodiment 1

[0056] As Figures 1-5 shown, this embodiment provides a continuous biological aerosol sampling device, which includes a sampling component, a liquid replenishment component, and a control component. The sampling component includes a sampling cup 32, a fan 20 located above it, and a sampling head 32. The sampling cup 32 is used to hold the sampling liquid. The fan 20 is used to generate an airflow rotating along the central axis direction of the sampling cup 32 and make the sampling liquid react with the particulate matter in the airflow to form an aerosol. The sampling head 32 is used to collect the aerosol sample. The liquid replenishment component includes a liquid storage bottle 4 and a peristaltic pump 5. The liquid storage bottle 4 is used to store the sampling liquid. The peristaltic pump 5 is used to continuously replenish the sampling liquid into the sampling cup 32. The control component includes a liquid shortage sensor 14 and a liquid level sensor. The liquid shortage sensor 14 is arranged between the liquid storage bottle 4 and the peristaltic pump 5. The liquid level sensor is arranged on the side wall of the sampling cup 32. The peristaltic pump 5 and the fan 20 control the start and stop according to the signals sent by the liquid shortage sensor 14 and the liquid level sensor to ensure the normal sampling process.

[0057] In this embodiment, there are both gas pipeline transportation and liquid pipeline transportation in the sampling cup 32. The gas pipeline transportation includes an air inlet 24, a blower 20, a sampling cup 32, and an air outlet 29. The liquid pipeline transportation includes a liquid storage bottle 4, an infusion tube 15, a peristaltic pump 5, a liquid replenishment port 23, a sampling cup 32, and a liquid outlet 37. The specific process is as follows: The control circuit board 18 fills the sampling liquid in the liquid storage bottle 4 into the sampling cup 32, and during the sampling process, the liquid level sensor on the sampling cup 32 is used to monitor the liquid volume in the sampling cup 32 in real time. When the liquid volume is insufficient, the sampling liquid is automatically replenished; when the liquid volume in the sampling cup 32 is too much or the liquid volume in the liquid storage bottle 4 is insufficient, the sampling is stopped, so that the sampling liquid in the sampling cup 32 is maintained within a stable liquid volume range.

[0058] In this embodiment, the blower 20 provides an air power source for the sampling of the sampling device. The high-speed rotating impeller accelerates the gas to meet the air flow requirement of the air inlet 24 of the instrument; after the sampling head 32 is connected to the sampling cup 32, the air flow inhaled by the blower 20 can flow spirally downward along the wall and the cylinder towards the sampling cup 32. When it reaches the bottom, a cyclone is formed upward at the center of the cup bottom and exits from the center of the cup top. The dust-containing gas will generate a centrifugal force during the rotation process, throwing the particles with a density greater than that of the gas towards the wall of the sampling cup 32, combining with the sampling liquid, thereby completing the sample collection.

[0059] In this embodiment, the main function of the peristaltic pump 5 is to transport the sampling liquid in the liquid storage bottle 4 to the sampling cup 32. The control circuit board 18 is connected to the USB interface 35 of the sampling cup 32 through a data line 33, and receives the liquid volume signal of the sampling cup 32 in real time, ensuring that the control circuit board 18 makes a feedback in real time according to the feedback information to ensure that the sampling liquid volume is maintained within the specified liquid volume range.

[0060] In this embodiment, the main function of the sampling cup 32 is to complete the combination of the sampling liquid and the air particulate matter to be measured, and monitor the liquid volume situation in the sampling cup 32 in real time, and transmit it to the control circuit board 18 in time, so that the sampling liquid is maintained within the specified liquid volume to ensure the normal progress of the sampling task. At the same time, when the sampling is completed, without disassembling the sampling cup 32, the sampled liquid can be taken out through the liquid outlet 37. The functions of the various components in the sampling cup 32 are as follows: The sampling cup 32 collects the particles thrown towards the cup wall under the action of centrifugal force, making them fully mixed with the sampling liquid to complete the collection and preservation of the sample; the high liquid level sensor 34 monitors whether the sampled liquid in the sampling cup 32 is too much; the USB interface 35 of the sampling cup 32 transmits the liquid volume information in the sampling cup 32 to the control circuit board 18; the low liquid level sensor 36 is responsible for monitoring whether the liquid volume in the sampling cup 32 reaches the specified range; the liquid outlet 37 can realize the acquisition of the sampled liquid that has been collected at any time without disassembling the device.

[0061] The replenishing liquid assembly further includes an infusion tube 15. A liquid replenishing port 23 is provided at the upper edge of the sampling cup 32. The infusion tube 15 is connected through the liquid storage bottle 4, the peristaltic pump 5, and the liquid replenishing port 23. Detection components are provided at both ends of the infusion tube 15 connected to the liquid replenishing port 23 and the liquid storage bottle 4 respectively.

[0062] In this embodiment, the infusion tube 15 is used to convey the sampling liquid automatically replenished to the sampling cup 32. The liquid shortage sensor 14 detects in real time whether there is sampling liquid in the infusion tube 15. When the liquid shortage sensor 14 detects that there is sampling liquid in the infusion tube 15, the sampling device operates normally; when the liquid shortage sensor 14 detects that there is no sampling liquid in the infusion tube 15, the liquid shortage sensor 14 sends an alarm signal to the control circuit board 18, and the control circuit board 18 terminates the continuous operation of the sampling device, and the fault indicator light is on.

[0063] In this embodiment, in addition to the liquid shortage sensor 14 arranged beside the peristaltic pump 5, detection components are also provided at the connection port of the infusion tube 15 and the liquid storage bottle 4 and at the connection port of the infusion tube 15 and the liquid replenishing port 23 of the sampling cup 32, so as to judge whether there is liquid flowing through different sections of the infusion tube 15, thereby quickly troubleshooting the specific fault location when the fault indicator light is on.

[0064] In this embodiment, the capacity of the liquid storage bottle 4 can be designed according to the actual situation. Since the higher the temperature and the lower the humidity, the faster the evaporation rate of the sampling liquid. Before customizing the capacity of the liquid storage bottle 4, a table of the evaporation rate of the sampling liquid under different temperature and humidity conditions can be measured, and according to the local temperature and humidity and the actual sampling time, the amount of sampling liquid that needs to be pre-stored in the liquid storage bottle 4 can be calculated. For example, when the temperature in the measured table is 25 °C and the humidity is 30RH, the evaporation rate of the sampling liquid is 0.5 ml / min, and the expected sampling time is 3 days, then at least 2.16 liters of sampling liquid (3 * 24 * 60 * 0.5 / 1000 = 2.16) needs to be pre-installed in the liquid storage bottle 4.

[0065] The blower 20 includes an air inlet 24 and an air outlet 29 arranged diagonally. The air inlet 24 is provided at the lower edge of the blower 20, corresponding to the position of the liquid replenishing port 23; the air outlet 29 is provided at the upper edge of the blower 20, corresponding to the position of the sampling head 32.

[0066] In this embodiment, when sampling starts, the fan 20 starts running simultaneously. The fan 20 drives air to enter from the air inlet 24. Under the action of the fan 20, the air flow accelerates and enters through the air inlets on the cup wall. Under the action of centrifugal force, it rotates downward along the cup wall of the sampling cup, forms an outer swirling flow downward along the sampling head 32 and moves downward along the axis of the sampling cup 32. The gas entering the bottom of the sampling cup 32 forms a cyclone upward in the axial direction of the central axis of the sampling cup 32, and finally passes through the fan 20 and is discharged from the air outlet 29. An effect of an outer cyclone downward and an inner cyclone upward is formed in the sampling cup, so that the dust-containing gas forms an aerosol after being thrown out.

[0067] The control assembly further includes a control circuit board 18 and a fault indicator light. The fault indicator light is used to indicate that the sampling liquid in the liquid storage bottle 4 is insufficient and the supplementary sampling liquid in the sampling cup 32 is full. The control circuit board 18 is connected to the peristaltic pump 5, the fan 20, the fault indicator light, the liquid shortage sensor 14, and the liquid level sensor, and is used to compare whether each signal coexists and analyze and process the sending order of each signal to control the normal operation of the sampling device.

[0068] In this embodiment, when the instrument is plugged into the power supply 12 and the instrument switch is turned on, the control circuit board 18 controls the fan 20 to run, and at the same time controls the peristaltic pump 5 to run forcibly for N seconds until the liquid shortage sensor 14 detects that there is liquid in the infusion tube 15. The control circuit board 18 receives the signal of the low liquid level sensor 36. When the low liquid level sensor 36 detects a no-liquid signal, the peristaltic pump 5 is started to supplement the liquid; when the low liquid level sensor 36 detects a liquid signal, the peristaltic pump 5 stops supplementing the liquid. If the low liquid level sensor 36 fails and the peristaltic pump 5 continuously supplements the liquid, resulting in an excessive amount of liquid in the sampling cup 32, when the control circuit board 18 receives the signal that the high liquid level sensor 34 detects liquid, the control circuit board 18 terminates the sampling, stops the fan 20 and the peristaltic pump 5 from running, and the fault indicator light lights up. During the sampling process, when the control circuit board 18 receives the signal that the liquid shortage sensor 14 detects no liquid in the infusion tube 15, the control circuit board 18 terminates the sampling, stops the fan 20 and the peristaltic pump 5 from running, and the fault indicator light lights up.

[0069] It further includes a wireless transmission module 16 and a remote monitoring system 38. The wireless transmission module 16 is arranged beside the control circuit board 18 and is used to transmit wireless signals to the remote monitoring system 38 to realize the real-time transmission of the working state data of the sampling device to the remote monitoring system 38.

[0070] In this embodiment, the wireless transmission module 16 adopts a wireless WiFi or 4G communication mode. During the sampling operation, each sensor continuously transmits signals to the control circuit board 18, and the control circuit board 18 simultaneously transmits signals to the remote monitoring system 38 through the wireless transmission module 16. The remote monitoring system 38 can monitor the working status of the sampling device, including the sampling start time, sampling operation time, sampling stop time, fault information, and alarm time, and store the working status data of the sampling device in the server. Embodiment 2

[0071] This embodiment also provides a method for continuous sampling of bioaerosols, which includes the sampling device described above:

[0072] S1. After connecting to the power supply 12, the fan 20 sucks the gas in the area to be sampled from the air inlet 24 on the side wall of the sampling cup 32.

[0073] S2. The gas sucked between the fan 20 and the sampling cup 32 is acted upon by the fan 20, and the dust-containing part in the gas is thrown towards the wall surface of the sampling cup 32 during rotation.

[0074] S3. After the fan 20 operates for M seconds, the sampling cup 32 starts to inject a quantitative sampling liquid, and after the sampling liquid contacts the wall surface, it reacts and combines with the dust particles.

[0075] S4. The fan 20 continues to operate and gradually forms a rotating air column, so that the sampling liquid that has reacted and combined with the dust particles forms an aerosol above the sampling cup 32.

[0076] S5. The sampling head 32 above the sampling cup 32 stays in the aerosol for sampling.

[0077] S6. The sampling head 32 that has completed sampling is replaced, the gas that has completed sampling is discharged from the air outlet 29 above the fan 20, and the sampling liquid that has completed the reaction is discharged from the liquid outlet 37 at the bottom of the sampling cup 32.

[0078] The specific steps of step S2 include:

[0079] S21. The fan 20 accelerates the gas sucked from the air inlet 24 by using a high-speed rotating impeller to meet the air flow requirement of the air inlet 24.

[0080] S22. After the sampling head 32 is connected to the sampling cup 32, the gas sucked by the fan 20 can flow spirally downward along the wall surface of the sampling cup 32 to form a cyclone.

[0081] S23. The dust-containing gas generates centrifugal force during rotation, throwing the particles with a density greater than that of the gas towards the wall surface of the sampling cup 32, causing them to combine with the sampling liquid, thereby completing the sampling.

[0082] The step S3 specifically includes:

[0083] S31. Turn on the instrument switch, the fan 20 starts running, and the peristaltic pump 5 starts running for N seconds until the liquid shortage sensor 14 detects that the infusion tube 15 is full of sample liquid, and sends a full liquid signal to the control circuit board 18; otherwise, the control circuit board 18 sends an alarm signal to the fault indicator after N seconds, indicating that the rehydration is abnormal;

[0084] S32. After receiving the full liquid signal, the control circuit board 18 controls the peristaltic pump 5 to continue running to fill the capacity P (L) of the sampling cup 32, and the t (s) is the time required for the peristaltic pump 5 to completely fill the sampling cup 32; wherein The S infusion tube 15 (m2) is the cross-sectional area of ​​the infusion tube 15, and the V (m / s) is the flow rate of the sample liquid in the peristaltic pump 5;

[0085] S33. During the operation of step S32, the low liquid level sensor 36 in the liquid level sensor detects the liquid level of the sampling cup 32 once: if the low liquid level sensor 36 sends a low-level liquid shortage signal to the control circuit board 18, the control circuit board 18 controls the peristaltic pump 5 to continue running for Δt seconds (Δt<t); if the low liquid level sensor 36 sends a low-level liquid full signal to the control circuit board 18, the control circuit board 18 controls the peristaltic pump 5 to stop after running for t seconds;

[0086] S34. During the detection process of step S33, the high liquid level sensor 34 in the liquid level sensor performs a secondary detection on the liquid level of the sampling cup 32; if the high liquid level sensor 34 sends a high-level liquid shortage signal to the control circuit board 18, the peristaltic pump 5 of step S32 continues to run; if the high liquid level sensor 34 sends a high-level liquid full signal to the control circuit board 18, the control circuit board 18 controls the peristaltic pump 5 to stop running immediately and sends an alarm signal to the fault indicator light;

[0087] S35. The sampling liquid in the sampling cup 32 is filled with the quantitative value P (L).

[0088] The specific steps of troubleshooting abnormal fluid replenishment in step S31 include:

[0089] S311. The fault indicator light is on, indicating that there is no liquid flowing in the infusion tube 15, and it is determined that the fluid infusion component is faulty;

[0090] S312. The control circuit board 18 confirms whether there is liquid at the place where the infusion tube 15 is connected to the liquid storage bottle 4. If not, it is determined that the cause of the fault is that the sampling liquid in the liquid storage bottle 4 is insufficient. The staff removes the liquid storage bottle 4 and releases the alarm state, and the fault indicator light is turned off; if so, the liquid storage bottle 4 is excluded from the fault, and the next step of the fault cause query is performed;

[0091] S313. The control circuit board 18 confirms whether there is liquid in the liquid infusion port 23 of the infusion tube 15 connected to the sampling cup 32. If so, it is determined that the fault is caused by the abnormality of the lack of liquid sensor 14. After the sampling liquid is injected into the sampling cup 32, the staff replaces the lack of liquid sensor 14, releases the alarm state, and turns off the fault indicator light; if not, the next step of fault cause inquiry is carried out;

[0092] S314. The control circuit board 18 confirms whether the peristaltic pump 5 is working normally. If so, it is determined that the cause of the fault is that the liquid shortage sensor 14 is abnormal. The staff will replace the liquid shortage sensor 14 after the sampling liquid in the sampling cup 32 is injected, and the alarm state is released, and the fault indicator light is turned off; if not, it is determined that the cause of the fault is that the peristaltic pump 5 is abnormal, the control circuit board 18 control switch is disconnected, and the staff will inspect and replace the peristaltic pump 5.

[0093] Before starting a detection in step S33, the specific installation steps of the liquid level sensor include:

[0094] S331. The sampling cup 32 is provided with three triangularly distributed holes before installation to facilitate installation of a device for detecting the position of the sampling liquid in the sampling cup 32;

[0095] S332. The liquid level sensor includes two, a low liquid level sensor 36, and a high liquid level sensor 34. After the electrodes of the two sensors are bent for the first time, they are inserted into the left and right holes in the reserved holes respectively;

[0096] S333. The electrodes of the two sensors are bent for the second time at the left and right holes respectively. After the two bending steps of the high liquid level sensor 34 are completed, they are inserted into the middle hole, so that the three electrodes of the two sensors are exposed outside the three reserved holes;

[0097] S334. Connect the three electrodes to the corresponding parts of the USB interface 35 of the sampling cup 32 through wires, put them into the card slot under the hole, and fix them with hot melt adhesive.

[0098] In this embodiment, before the instrument is started, it is necessary to fill the sampling liquid into the liquid storage bottle 4, screw the sampling cup 32 clockwise and tightly install it on the sampling head 31, connect the liquid storage bottle 4 and the peristaltic pump 5 with the infusion tube 15, insert the infusion tube 15 into the needle hole of the liquid replenishing port 23 of the sampling head 31, and connect the USB interfaces of each sensor with the data line. Connect the power adapter to the power supply 12, turn on the instrument switch, and the control circuit board 18 controls the peristaltic pump 5 and the blower 20 to start running at the same time. The sampling liquid is transported from the liquid storage bottle 4 to the liquid replenishing port 23 through the infusion tube 15. When the sample liquid flows out, under the action of the blower 20, it enters the sampling head 31 together with the air sucked from the air inlet 24. During this process, the input sample liquid will be fully mixed with the large particles thrown out by the centrifugal force in the air and enter the sampling cup 32 in a spiral shape to complete the sampling. During the operation of the device, if the liquid shortage sensor 14 detects that there is no liquid in the infusion tube 15, it will send a signal to the control panel 18, indicating that the amount of sampling liquid in the liquid storage bottle 4 is insufficient. Then the electronic control unit controls the instrument to stop running, and at the same time, the fault indicator light 39 lights up; if the amount of liquid in the sampling cup 32 is too much, it can be detected by the high liquid level sensor 34, and the signal transmitted by it to the electronic control unit will also cause the electronic control module to control the instrument to stop running, and the fault indicator light 39 lights up. When it is necessary to take out the sample for inspection, turn off the instrument switch, stop sampling, take out the sample liquid in the sampling cup 32 from the liquid outlet 37 and transfer it to a cryopreservation tube for storage or submission for inspection, and then turn on the device again to start a new round of sampling.

[0099] Obviously, the above-mentioned embodiments of the present invention are only examples for clearly explaining the technical solutions of the present invention, rather than limitations on the specific implementation manners of the present invention. Any modifications, equivalent replacements, and improvements made within the spirit and principle of the claims of the present invention shall be included in the protection scope of the claims of the present invention.

Claims

1. A bioaerosol continuous sampling device, characterized in that: It includes a sampling component, a fluid replenishment component, and a control component. The sampling component includes a sampling cup, a fan located above the sampling cup, and a sampling head. The sampling cup is used to receive the sampling liquid. The fan is used to generate an airflow rotating along the central axis of the sampling cup and allow the sampling liquid to react with particulate matter in the airflow to form an aerosol. The sampling head is used to collect aerosol samples. The fluid replenishment component includes a liquid storage bottle and a peristaltic pump. The liquid storage bottle is used to store the sampling liquid. The peristaltic pump is used to continuously replenish the sampling liquid into the sampling cup. The control component includes a liquid shortage sensor and a liquid level sensor. The liquid shortage sensor is arranged between the liquid storage bottle and the peristaltic pump. The liquid level sensor is arranged on the side wall of the sampling cup. The peristaltic pump and the fan are controlled to start and stop according to the signals sent by the liquid shortage sensor and the liquid level sensor to achieve the normal sampling process.

2. A bioaerosol continuous sampling device according to claim 1, characterized in that: The infusion component also includes an infusion tube. A infusion port is provided on the upper edge of the sampling cup. The infusion tube is connected to the liquid storage bottle, the peristaltic pump and the infusion port. Detection components are provided at both ends of the infusion tube which are respectively connected to the infusion port and the liquid storage bottle.

3. A bioaerosol continuous sampling device according to claim 2, characterized in that: The fan comprises an air inlet and an air outlet arranged diagonally, wherein the air inlet is arranged at the lower edge of the fan, corresponding to the position of the liquid infusion port; The air outlet is arranged at the upper edge of the fan, corresponding to the position of the sampling head.

4. A bioaerosol continuous sampling device according to claim 1, characterized in that: The control component also includes a control circuit board and a fault indicator light. The fault indicator light is used to indicate that there is insufficient sampling liquid in the liquid storage bottle and that the sampling cup is full of supplementary sampling liquid. The control circuit board is connected to the peristaltic pump, the fan, the fault indicator light, the liquid shortage sensor, and the liquid level sensor, and is used to compare whether various signals coexist and analyze and process the sending order of various signals to control the normal operation of the sampling device.

5. A bioaerosol continuous sampling device according to claim 4, characterized in that: It also includes a wireless transmission module and a remote monitoring system. The wireless transmission module is arranged beside the control circuit board and is used to transmit wireless signals to the remote monitoring system to realize real-time transmission of the working status data of the sampling device to the remote monitoring system.

6. A method for continuous sampling of bioaerosols, characterized in that: A sampling device comprising any one of claims 1 to 5: S1. After the power is connected, the fan draws the gas in the area to be sampled from the air inlet located on the side wall of the sampling cup; S2. The gas sucked between the fan and the sampling cup is thrown toward the wall of the sampling cup during the rotation of the fan. S3. After the fan runs for M seconds, the sampling cup begins to inject a quantitative sampling liquid, and the sampling liquid reacts and combines with the dust particles after contacting the wall surface; S4. The fan continues to run and gradually forms a rotating wind column, so that the sample liquid reacts with the dust particles to form an aerosol above the sampling cup; S5. The sampling head located above the sampling cup remains in the aerosol for sampling; S6. The sampling head that has completed sampling is replaced, and the gas that has completed sampling is discharged from the air outlet located above the fan, and the sampling liquid that has completed the reaction is discharged from the liquid outlet at the bottom of the sampling cup.

7. A method for continuous sampling of bioaerosols according to claim 6, characterized in that: The step S2 specifically includes: S21. The fan accelerates the gas sucked into the air inlet by means of a high-speed rotating impeller to meet the air flow demand of the air inlet; S22. After the sampling head is connected to the sampling cup, the gas sucked by the fan can flow downward in a spiral along the wall of the sampling cup to form a cyclone; S23. The dust-laden gas generates centrifugal force during the rotation process, which throws particles with density greater than that of the gas toward the wall of the sampling cup, allowing them to combine with the sampling liquid, thus completing the sampling.

8. The method for continuous sampling of bioaerosol according to claim 1, characterized in that: The step S3 specifically includes: S31. Turn on the instrument switch, start the fan, and start the peristaltic pump for N seconds until the liquid shortage sensor detects that the infusion tube is full of sample liquid and sends a full liquid signal to the control circuit board; otherwise, the control circuit board sends an alarm signal to the fault indicator after N seconds, indicating that the rehydration is abnormal; S32. After receiving the full liquid signal, the control circuit board controls the peristaltic pump to continue running to fill the capacity P (L) of the sampling cup, and t (s) is the time required for the peristaltic pump to completely fill the sampling cup; wherein The S 输液管 (m 2 ) is the cross-sectional area of ​​the infusion tube, and V (m / s) is the flow rate of the sample liquid in the peristaltic pump; S33. During the operation of step S32, the low liquid level sensor in the liquid level sensor detects the liquid level of the sampling cup once: if the low liquid level sensor sends a low-level liquid shortage signal to the control circuit board, the control circuit board controls the peristaltic pump to continue running for Δt seconds (Δt<t); if the low liquid level sensor sends a low-level liquid full signal to the control circuit board, the control circuit board controls the peristaltic pump to stop after running for t seconds; S34. During the detection process of step S33, the high liquid level sensor in the liquid level sensor performs a secondary detection on the liquid level of the sampling cup; if the high liquid level sensor sends a high-level liquid shortage signal to the control circuit board, the peristaltic pump of step S32 continues to run; if the high liquid level sensor sends a high-level liquid full signal to the control circuit board, the control circuit board controls the peristaltic pump to stop running immediately and sends an alarm signal to the fault indicator light; S35. The sampling liquid in the sampling cup is filled with the quantitative value P(L).

9. A method for continuous sampling of bioaerosols according to claim 8, characterized in that: The specific steps of troubleshooting abnormal fluid replenishment in step S31 include: S311. The fault indicator light is on, indicating that there is no liquid flowing in the infusion tube, and it is determined that the fluid infusion component is faulty; S312. The control circuit board confirms whether there is liquid at the place where the infusion tube is connected to the liquid storage bottle. If not, it is determined that the cause of the fault is insufficient sampling liquid in the liquid storage bottle. The staff removes the liquid storage bottle and releases the alarm state, and the fault indicator light turns off; if yes, the liquid storage bottle fault is eliminated, and the next step of fault cause inquiry is performed; S313. The control circuit board confirms whether there is liquid in the infusion port of the infusion tube connected to the sampling cup. If so, it is determined that the fault is caused by an abnormality in the lack of liquid sensor. After the sampling liquid is injected into the sampling cup, the staff replaces the lack of liquid sensor, releases the alarm state, and turns off the fault indicator light; if not, the next step is to query the cause of the fault; S314. The control circuit board confirms whether the peristaltic pump is working normally. If so, it is determined that the cause of the fault is an abnormality in the liquid shortage sensor. After the sampling liquid in the sampling cup is injected, the staff will replace the liquid shortage sensor, release the alarm state, and turn off the fault indicator light. If not, it is determined that the cause of the fault is an abnormality in the peristaltic pump, the control switch of the control circuit board is disconnected, and the staff will inspect and replace the peristaltic pump.

10. A method for continuous sampling of bioaerosols according to claim 8, characterized in that: Before starting a detection in step S33, the specific installation steps of the liquid level sensor include: S331. The sampling cup is provided with three triangularly distributed holes before installation to facilitate installation of a device for detecting the position of the sampling liquid in the sampling cup; S332. The liquid level sensor includes two, a low liquid level sensor and a high liquid level sensor. After the electrodes of the two sensors are bent for the first time, they are inserted into the left and right holes in the reserved holes respectively; S333. The electrodes of the two sensors are bent for the second time at the left and right holes respectively. After the two bending processes of the high liquid level sensor are completed, it is inserted into the middle hole, so that the three electrodes of the two sensors are exposed outside the three reserved holes; S334. Connect the three electrodes to the corresponding parts of the USB interface of the sampling cup through wires, put them into the card slot under the hole, and fix them with hot melt adhesive.

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