Liquid chromatographic analyzer with multi-channel chromatographic column

Abstract

The invention discloses a liquid chromatographic analyzer with a multichannel chromatographic column, and relates to the technical field of chromatographic analysis, the liquid chromatographic analyzer comprises a liquid conveying system, a sample injection system, a separation system and a detection system, the separation system is used for separating components of a sample, and the separation system comprises the chromatographic column; the chromatographic column is internally provided with a main flow channel and an auxiliary flow channel through which liquid flows; the chromatographic column is also provided with an adjusting assembly and a switching assembly; the liquid flows out of the main flow channel or the auxiliary flow channel through the adjustment of the adjusting assembly; the switching assembly is used for switching the liquid flowing direction and carrying out back-flushing cleaning on the interior of the chromatographic column; the detection system is used for detecting liquid and sample component data flowing out of the chromatographic column and sending the detection data to the computer and the printing system, and the printing system prints a chromatogram. By arranging the components, the distribution uniformity of a liquid phase is effectively improved, the detection requirements of different flow rates are met, the backwashing cleaning function is added, and the operation convenience of the chromatograph is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of chromatographic analysis, and particularly to a liquid chromatograph with a multi-channel chromatographic column. Background Art

[0002] As the core technology for analyzing the chemical components of traditional Chinese medicine, ultra-high performance liquid chromatography (UPLC) is directly related to the separation efficiency and the performance of the chromatographic column. However, the chemical components of traditional Chinese medicine are characterized by high complexity, wide molecular weight distribution, and high viscosity of some components, such as polysaccharides, colloids, and some alkaloid components, and their mobile phase viscosity is significantly higher than that of conventional analytical samples.

[0003] During chromatographic analysis of traditional Chinese medicine liquid phase, small solid impurities are likely to remain inside, resulting in blockage of the chromatographic column. When traditional UPLC chromatographic columns are used to handle traditional Chinese medicine liquid phase, there is generally a problem of low backflushing cleaning efficiency.

[0004] Conventional chromatographic columns use fillers with a fixed pore structure and homogeneous filling technology. When analyzing high-viscosity samples, the column pressure is likely to rise abnormally due to the increased mass transfer resistance of the mobile phase. It is necessary to disassemble the chromatographic column and replace the flow control plate with a corresponding available area according to the viscosity of the sample to be detected, and the operation is very troublesome. Moreover, the traditional backflushing operation requires disassembling the chromatographic column and swapping the inlet and outlet. Multiple disassembly operations are likely to cause loosening of the end of the chromatographic column, resulting in leakage under high pressure during use and affecting the accuracy of experimental data.

[0005] The above problems exist in the use of traditional chromatographic columns, and the flow rate of the chromatographic column cannot be adjusted according to experimental requirements, resulting in uneven flow of the liquid phase in the filler at low flow rates, poor peak symmetry of the chromatographic column, and affecting experimental results.

[0006] Therefore, it is necessary to invent a liquid chromatograph with a multi-channel chromatographic column to solve the above problems. Summary of the Invention

[0007] The purpose of the present invention is to provide a liquid chromatograph with a multi-channel chromatographic column to solve the problems raised in the above background art.

[0008] To achieve the above object, the present invention provides the following technical solutions: A liquid chromatography analyzer with a multi-channel chromatographic column, comprising: a liquid delivery system for delivering mobile phase liquid; a sample injection system for injecting a sample and mixing it with the mobile phase liquid; a separation system for separating the components of the sample, the separation system including a chromatographic column; a main flow channel and a secondary flow channel for liquid circulation are provided in the chromatographic column; the chromatographic column is further provided with an adjustment component and a switching component; the sample input by the sample injection system can flow out from the main flow channel or the secondary flow channel through the adjustment of the adjustment component; when the liquid flows out from the main flow channel, it can make the flow rate of the sample uniform in the packing; when the liquid flows out from the secondary flow channel, it is convenient for viscous liquid to flow through; a switching component, which is arranged in the chromatographic column and cooperates with the adjustment component to switch the liquid flow direction and perform backflush cleaning on the inside of the chromatographic column; a detection system for detecting the data of the sample components flowing out of the chromatographic column, sending the detection data to a computer, and outputting a chromatogram.

[0009] Preferably, both the main flow channel and the secondary flow channel are opened on one side of the chromatographic column close to the output end; the main flow channel and the secondary flow channel have the same number and are arranged axially staggered with each other; both the main flow channel and the secondary flow channel extend radially and expand to both sides; the area occupied by the main flow channel is smaller than the area occupied by the secondary flow channel.

[0010] Preferably, the adjustment component includes: a switching tube that can selectively communicate with the main flow channel / secondary flow channel; a switching sleeve that is rotatably sleeved on the outside of the chromatographic column and is fixedly connected to the switching tube for adjusting the communication state between the switching tube and the main flow channel / secondary flow channel.

[0011] Preferably, the chromatographic column further includes: a column tube, one end of which is connected to a liquid inlet tube, the other end of the liquid inlet tube is connected to the liquid inlet end of the chromatographic column, the other end of the column tube is connected to a liquid outlet tube, and the other end of the liquid outlet tube is connected to the liquid outlet end of the chromatographic column; a filtering part, which is placed inside the column tube and fixedly connected to both ends of the column tube for filtering impurities in the liquid and the sample.

[0012] Preferably, the switching component includes: an upper liquid tube and a lower liquid tube, both of which are placed outside the column tube; a first valve body for controlling the on / off of the liquid inlet tube; a second valve body for controlling the on / off of the upper liquid tube; a third valve body for controlling the on / off of the liquid outlet tube; a fourth valve body for controlling the on / off of the lower liquid tube.

[0013] Preferably, when the first valve body and the third valve body are conducted, the sample and the liquid pass through the liquid inlet tube, the column tube, and the liquid outlet tube in sequence for sample separation; when the second valve body and the fourth valve body are conducted, the liquid passes through the liquid inlet tube, the upper liquid tube, the column tube, the lower liquid tube, and the liquid outlet tube in sequence for backflush cleaning of the chromatographic column.

[0014] Preferably, the groove depth in the central region of the main flow channel and the secondary flow channel is greater than that in the edge region, suppressing the central flow velocity and reducing the flow resistance.

[0015] Preferably, the end of the main flow channel extends to the inside of the column tube, and the end of the secondary flow channel approaches the inside of the column tube.

[0016] Technical effects and advantages of the present invention: 1. By providing a column tube, a filtering part, a main flow channel, a secondary flow channel and an adjusting component, the present invention effectively achieves the purpose of improving the uniformity of liquid phase distribution. The filtering part on the side of the water inlet pipe is of a multi-layer structure, and the density increases sequentially from top to bottom, improving the uniformity of liquid distribution in the column tube and filtering impurities in the liquid phase. And through the cooperation of the adjusting component with the main flow channel / secondary flow channel, it is realized that when the liquid flow velocity in the column tube is different, the sample can still be evenly distributed, improving the peak symmetry of the chromatographic column and achieving the purpose of being applicable to various flow rates.

[0017] 2. By providing a main flow channel and a secondary flow channel, the present invention realizes the purpose of being applicable to viscous liquid phases. The depth of the main flow channel and the secondary flow channel gradually changes from the center to the edge region, which can suppress the too fast central flow velocity and reduce the flow resistance; the liquid enters the liquid inlet through the secondary flow channel, and the proportion area of the secondary flow channel is larger than that of the main flow channel, and high-viscosity liquid phases can pass through the packing, reducing the risk of blockage; it can also be applicable to the liquid flow rates between high flow rates and low flow rates.

[0018] 3. By providing a switching component, the present invention effectively achieves the backflushing effect. During use, by controlling the conduction states of the first valve body, the second valve body, the third valve body and the fourth valve body, the liquid flows back from the water outlet end of the column tube to the water inlet end, and the packing, the main flow channel, the secondary flow channel and the filtering part inside are backflushed and cleaned.

[0019] 4. By providing an outer sleeve, the safety of the chromatographic column is improved. The outer sleeve is sleeved outside the column tube to protect the column tube and prevent the packing from moving due to bumping. There is a gap between the outer sleeve and the column tube, enabling it to play a buffering role; and by providing a guiding ring and a scale, the purpose of facilitating the judgment of the working state of the chromatographic column is achieved. Description of the drawings

[0020] Figure 1 is a flow chart of the present invention.

[0021] Figure 2 is a schematic diagram of the overall structure of the chromatographic column in the present invention.

[0022] Figure 3 is a cross-sectional view of the chromatographic column in the present invention.

[0023] Figure 4Explosion schematic diagram of the top of the chromatographic column in the present invention.

[0024] Figure 5 Explosion schematic diagram of the bottom of the chromatographic column in the present invention.

[0025] Figure 6 Structural schematic diagram of the adjustment component in the present invention.

[0026] Figure 7 Distribution schematic diagram of the main flow channel and the secondary flow channel in the present invention.

[0027] Figure 8 Cross-sectional view of the adjustment component in the present invention.

[0028] Figure 9 Position schematic diagram of the lower liquid pipe and the upper liquid pipe in the present invention.

[0029] Figure 10 Schematic diagram of the liquid flow direction during the operation and backflushing of the chromatographic column in the present invention.

[0030] In the figure: 1. Liquid delivery system; 2. Sampling system; 3. Separation system; 4. Chromatographic column; 41. Main flow channel; 42. Secondary flow channel; 43. Adjustment component; 431. Switching pipe; 432. Switching sleeve; 433. Liquid inlet notch; 44. Switching component; 441. Upper liquid pipe; 442. Lower liquid pipe; 443. First valve body; 444. Second valve body; 445. Third valve body; 446. Fourth valve body; 5. Detection system; 6. Computer; 7. Column tube; 8. Liquid inlet pipe; 9. Liquid outlet pipe; 10. Filter part; 11. Connector; 12. Connection sleeve; 13. Conical port. Detailed implementation manners

[0031] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0032] Embodiment 1, the present invention provides as Figures 1 to 8The liquid chromatography analyzer with a multi-channel chromatographic column shown in the figure includes an infusion system 1 for delivering a liquid, i.e., the mobile phase; an injection system 2 for inputting a test sample and mixing it with the mobile phase; a separation system 3 for separating the components of the sample; a detection system 5 for detecting the liquid and sample component data flowing out of the chromatographic column 4 and sending the detection data to a computer 6 and a printing system, and the printing system prints a chromatogram. The infusion system 1, the injection system 2, and the detection system 5 are all prior arts, and their specific structures will not be described further. The computer 6 mainly analyzes and processes the detection data, and its analysis and processing method is also a prior art.

[0033] The separation system 3 includes a chromatographic column 4 and may also include other components for assisting separation, such as a thermostat for controlling the temperature of the chromatographic column 4.

[0034] The chromatographic column 4 is provided with a main flow channel 41 and a sub-flow channel 42 for the liquid to flow through. The chromatographic column 4 is filled with a packing material, i.e., the stationary phase. Both the main flow channel 41 and the sub-flow channel 42 are located at the output end of the packing material in the chromatographic column 4. The chromatographic column 4 is also provided with an adjustment component 43 and a switching component 44, and the flow direction of the liquid and the sample is changed through the adjustment component 43.

[0035] The sample input by the injection system 2 can flow out from the main flow channel 41 or the sub-flow channel 42 through the adjustment of the adjustment component 43. It should be noted that the sample follows the liquid input by the infusion system 1. When the liquid flows out from the main flow channel 41, the sample has a uniform flow rate in the packing material. During the process of the liquid flowing out through the main flow channel 41, the sample is subjected to resistance at the center position of the packing material, resulting in a decrease in the flow rate at the center position and an increase in the flow rate at the edge position, enabling the sample to flow uniformly in the packing material and improving the peak shape symmetry.

[0036] When the liquid flows out from the sub-flow channel 42, it is convenient for viscous liquids to flow through. The sub-flow channel 42 is more diffusely distributed and has a larger proportion of the area, enabling viscous liquids to also flow through the packing material uniformly and stably, improving the applicable range of the chromatographic column 4.

[0037] Specifically, both the main flow channel 41 and the sub-flow channel 42 are opened on one side of the chromatographic column 4 close to the output end. The liquid and the sample first pass through the packing material and then enter the main flow channel 41 or the sub-flow channel 42. The main flow channel 41 and the sub-flow channel 42 have the same number and are arranged axially staggered with each other. The groove depth in the central region of the main flow channel 41 and the sub-flow channel 42 is greater than that in their edge regions, suppressing the central flow rate and reducing the flow resistance. During use, the flow rate of the liquid in the middle of the packing material is limited, making the sample more evenly distributed at the same height position in the packing material.

[0038] The main flow channel 41 and the secondary flow channels 42 both extend radially and expand towards both sides. The main flow channel 41 expands a longer distance towards both sides but has a smaller expanded area. The secondary flow channels 42 expand a shorter distance towards both sides but have a larger expanded area. The area occupied by the main flow channel 41 is smaller than that occupied by the secondary flow channels 42. The setting of the secondary flow channels 42 makes it easier for viscous liquids to pass through. Since the secondary flow channels 42 occupy a larger area, when non-viscous liquids pass through the secondary flow channels 42, the liquid passes through at a faster speed, which is suitable for high-flow situations, enabling the chromatographic column 4 to meet various flow requirements.

[0039] More specifically, the adjustment assembly 43 includes: a switching tube 431 that can selectively communicate with the main flow channel 41 / secondary flow channels 42. The switching tube 431 is placed at the center of the axial distribution of the main flow channel 41 and the secondary flow channels 42. The upper end of the switching tube 431 is provided with liquid inlet notches 433, and the number of them is the same as that of the main flow channel 41 / secondary flow channels 42. During use, by rotating the switching tube 431 to an appropriate angle, the liquid inlet notches 433 can be made to correspond to and be interlocked with the main flow channel 41 / secondary flow channels 42. A switching sleeve 432 is rotatably sleeved outside the chromatographic column 4 and is fixedly connected to the switching tube 431, used to adjust the communication state between the switching tube 431 and the main flow channel 41 / secondary flow channels 42. The switching sleeve 432 has the function of protecting the column tube 7 to prevent the packing from moving due to bumps. During use, by rotating the switching sleeve 432, the switching sleeve 432 rotates relative to the column tube 7, thereby adjusting the relative angle of the switching tube 431 relative to the main flow channel 41 / secondary flow channels 42.

[0040] It should be noted that in actual use, in addition to setting the main flow channel 41 and the secondary flow channels 42, channels such as a third flow channel and a fourth flow channel for guiding the flow of liquids can also be set according to requirements to achieve the purpose of applying to more types of liquids. And the distribution area and shape of the corresponding flow channels are set according to the properties of the targeted liquids to increase the applicable liquids. At the same time, the liquid inlet notches 433 opened at the upper end of the switching tube 431 are also modified synchronously so that when the switching tube 431 rotates, only a single flow channel can be made to conduct.

[0041] Specifically, the chromatographic column 4 further includes: a column tube 7, one end of which is connected to a liquid inlet tube 8, the other end of the liquid inlet tube 8 is connected to the liquid inlet end of the chromatographic column 4, the other end of the column tube 7 is connected to a liquid outlet tube 9, the other end of the liquid outlet tube 9 is connected to the liquid outlet end of the chromatographic column 4, and the packing is placed inside the column tube 7; a filtering part 10, which is placed inside the column tube 7 and fixedly connected to both ends of the column tube 7, used to filter impurities in the liquid and the sample. The filtering part 10 near the liquid inlet tube 8 is a multi-layer structure, and the density increases sequentially from top to bottom, improving the uniformity of liquid distribution and filtering impurities in the liquid. The filtering part 10 near the liquid outlet tube 9 is only used to fix the packing.

[0042] It should be noted that the main flow channel 41 and the secondary flow channel 42 are arranged between the liquid outlet pipe 9 and the corresponding filtering part 10.

[0043] More specifically, the end of the main flow channel 41 extends to the inside of the column tube 7, and the end of the secondary flow channel 42 approaches the inside of the column tube 7. Therefore, during use, it effectively prevents liquid from entering the gap between the packing and the column tube 7 circumferentially through the packing, causing some liquid to enter the non-operating main flow channel 41 / secondary flow channel 42, resulting in some components of the sample not being able to be discharged from the chromatographic column 4 in time or being unable to be discharged from the chromatographic column 4, affecting the accurate value of the experimental data.

[0044] Specifically, the chromatographic column 4 further includes connecting heads 11, which are respectively rotatably connected to both ends of the switching sleeve 432 and are used to connect the pipes for the liquid to enter and exit the chromatographic column 4. The connecting heads 11 can be designed in the shape of hexagon bolts for easy connection of the pipes, but the specific shape is not particularly limited in this application; the column tube 7 is fixedly connected to the connecting heads 11; a connecting sleeve 12 is fixedly connected to the inside of each connecting head 11, and a tapered opening 13 is provided in the middle of the connecting sleeve 12. The end of the tapered opening 13 communicates with the corresponding liquid inlet pipe 8 and liquid outlet pipe 9. The setting of the tapered opening 13 can make the connection between the pipe and the connecting sleeve 12 tighter, enabling it to withstand higher pressures. It should be noted that threads are provided at the ends of the connecting heads 11 for easy connection of external pipes, and the threads are not shown in the figure.

[0045] More specifically, a toothed structure is provided on the outer side of the switching sleeve 432, which can drive the switching sleeve 432 to rotate through driving components such as a stepper motor or a servo motor. The toothed structure, the stepper motor, and the servo motor are all prior arts and will not be described in detail here and are not shown in the figure either. The stepper motor or the servo motor is connected to the computer 6 through an electrical signal.

[0046] In summary, connect the pipes for the output liquid of the liquid delivery system 1 and the sample of the sample injection system 2 to the connecting heads 11, make the pipes communicate with the corresponding connecting sleeves 12, and connect the pipe for inputting to the detection system 5 to another connecting sleeve 12. Then, adjust the switching tube 431 to communicate with the main flow channel 41 / secondary flow channel 42 according to the experimental requirements.

[0047] When using a liquid with a low flow rate in the experiment, rotate the switching sleeve 432 to make the switching tube 431 communicate with the main flow channel 41, and then make the separated sample enter the detection system 5; when using a viscous liquid in the experiment, rotate the switching sleeve 432 to make the switching tube 431 communicate with the secondary flow channel 42 to make the separated sample enter the detection system 5; when the experiment requires increasing the liquid flow rate, still make the liquid pass through the secondary flow channel 42 so that the non-viscous liquid can stably and evenly pass through the separation of the chromatographic column 4 and enter the detection system 5.

[0048] Embodiment 2. Through the above embodiment, it is found in actual work that the chromatographic column is prone to blockage during operation. Especially when using liquids or samples with fine particles, the blockage of the chromatographic column will cause the pressure to rise abnormally, resulting in the overload alarm or shutdown of the pump for transporting the liquid, and even damage the flow path of the chromatographic system. It will also cause abnormal peak shape and decreased separation efficiency, resulting in inaccurate chromatogram data and affecting the detection results. Therefore, for the problem of chromatographic column blockage, a flushing solution is generally used to backflush the chromatographic column.

[0049] Specifically, as Figures 7 to 10 , it further includes a switching component 44, which is arranged inside the chromatographic column 4 and cooperates with the adjusting component 43 to switch the liquid flow direction and perform backflushing cleaning on the inside of the chromatographic column 4. The switching component 44 realizes the cleaning of the chromatographic column 4 by changing the liquid flow direction inside the chromatographic column 4. When the traditional chromatographic column 4 is backflushed, it is necessary to manually disassemble the chromatographic column 4, change the direction and reinstall it. Multiple installations are likely to cause damage to the connecting components at the end of the chromatographic column 4, resulting in liquid leakage and damage to the chromatographic column 4.

[0050] More specifically, the switching component 44 includes: an upper liquid pipe 441 and a lower liquid pipe 442, both of which are placed outside the column tube 7. The upper liquid pipe 441 and the lower liquid pipe 442 are used to switch the liquid flow direction; a first valve body 443, which is used to control the on-off of the liquid inlet pipe 8 and control whether the liquid and the sample can enter the column tube 7 through the liquid inlet pipe 8; a second valve body 444, which is used to control the on-off of the upper liquid pipe 441 and control whether the liquid can enter the column tube 7 through the output end of the column tube 7; a third valve body 445, which is used to control the on-off of the liquid outlet pipe 9 and control whether the liquid and the sample can be discharged from the column tube 7 through the liquid outlet pipe 9; a fourth valve body 446, which is used to control the on-off of the lower liquid pipe 442 and control whether the liquid can be discharged from the column tube 7 through the input end of the column tube 7.

[0051] It should be noted that the first valve body 443, the second valve body 444, the third valve body 445 and the fourth valve body 446 are all electrically controlled valves and are electrically connected to the computer 6. The on-off of the first valve body 443, the second valve body 444, the third valve body 445 and the fourth valve body 446 is controlled by the computer 6.

[0052] Specifically, when the first valve body 443 and the third valve body 445 are conducting, the second valve body 444 and the fourth valve body 446 are closed. The sample and the liquid pass through the liquid inlet pipe 8, the column tube 7, and the liquid outlet pipe 9 in sequence for sample separation. At this time, it is the first state, that is, the normal working state of the chromatographic column 4, as Figure 10 the a state in

[0053] When the second valve body 444 is in communication with the fourth valve body 446, the first valve body 443 and the third valve body 445 are closed, and the liquid sequentially passes through the liquid inlet pipe 8, the upper liquid pipe 441, the column pipe 7, the lower liquid pipe 442, and the liquid outlet pipe 9 to perform backflushing and cleaning of the chromatographic column 4. At this time, it is in the second state, that is, the backflushing state of the chromatographic column 4, as Figure 10 the b state in

[0054] It should be noted that when the main flow channel 41 is blocked during operation, during backflushing, the liquid inlet notch 433 can be made to correspond to the main flow channel 41 to perform separate backflushing on the main flow channel 41, and perform short-term maintenance backflushing on the sub-flow channel 42; similarly, the sub-flow channel 42 can also be separately backflushed to improve the backflushing and cleaning effect, shorten the backflushing and cleaning time, and reduce the liquid waste caused by backflushing.

[0055] It should be pointed out that through the dispersed arrangement of the main flow channel 41 / sub-flow channel 42, during the backflushing process, the flushing liquid can enter the column pipe 7 dispersedly through the main flow channel 41 / sub-flow channel 42 to uniformly backflush the packing inside it, avoiding the situation that after the flushing liquid for backflushing enters the column pipe 7, it cannot reach the edge position of the packing, resulting in ineffective backflushing and cleaning of the circumferential direction of the packing; due to the different distribution areas and positions of the main flow channel 41 / sub-flow channel 42, the flushing pressure received by the packing during backflushing and cleaning is also different. The expansion area of the main flow channel 41 is smaller, enabling the flushing liquid to enter the column pipe 7 with a relatively high pressure and backflush the packing. The expansion area of the sub-flow channel 42 is larger, enabling the flushing liquid to be more evenly distributed when entering the column pipe 7, and the flushing area received by the packing is larger. In actual use, the main flow channel 41 / sub-flow channel 42 can also be alternately communicated with the liquid inlet notch 433 according to the blockage situation to achieve the purpose of overall backflushing of the packing. Compared with the traditional backflushing method, the backflushing effect is better (because in the traditional backflushing method, the flushing liquid generally enters from the liquid outlet and flows in the reverse direction for flushing, and the flushing area and path depend on the size of the liquid inlet, while generally the liquid outlet is relatively small, which is prone to leaving a large area of flushing dead corners, resulting in poor backflushing effect).

[0056] In summary, when the chromatographic column 4 is operating normally, the first valve body 443 and the third valve body 445 are in communication, and the second valve body 444 and the fourth valve body 446 are closed. At the same time, the liquid drives the sample to sequentially pass through the liquid inlet pipe 8, the column pipe 7, the main flow channel 41 / sub-flow channel 42, the switching pipe 431, and the liquid outlet pipe 9, and then the separated sample enters the detection system 5.

[0057] When the chromatographic column 4 is blocked, the first valve body 443 and the third valve body 445 are closed, and the second valve body 444 and the fourth valve body 446 are conducted. Subsequently, a cleaning liquid is injected into the chromatographic column 4 through the liquid inlet system. Then, the liquid sequentially passes through the liquid inlet pipe 8, the upper liquid pipe 441, the column tube 7, the lower liquid pipe 442, and the liquid outlet pipe 9 to perform backflushing cleaning on the chromatographic column 4. At the same time, by rotating the switching sleeve 432, the liquid can respectively pass through the main flow channel 41 and the sub-flow channel 42 to perform backflushing on the packing and the filtering part 10. When the main flow channel 41 or the sub-flow channel 42 is blocked, individual channels can also be backflushed separately, shortening the backflushing time. It is also possible to alternately connect the main flow channel 41 / sub-flow channel 42 with the liquid inlet notch 433 according to the blockage situation to achieve comprehensive backflushing cleaning of the entire packing and improve the cleaning effect of the chromatographic column.

[0058] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A liquid chromatograph having a multi-channel chromatographic column, characterized in that: include: Infusion system, used to deliver mobile phase liquid; Injection system, used to input sample and mix it with mobile phase liquid; A separation system for separating components of a sample, the separation system comprising a chromatographic column; the chromatographic column is provided with a main flow channel and a secondary flow channel for liquid circulation; the chromatographic column is also provided with a regulating component and a switching component; The sample input by the injection system can flow out from the main channel or the secondary channel through the adjustment of the adjustment component; when the liquid flows out from the main channel, the flow rate of the sample in the packing can be uniform; When the liquid flows out from the secondary flow channel, it is convenient for the viscous liquid to flow; A switching component is disposed in the chromatographic column and cooperates with the regulating component to switch the flow direction of the liquid and perform backwash cleaning on the inside of the chromatographic column; The detection system is used to detect the component data of the sample flowing out of the chromatographic column, send the detection data to the computer, and output the chromatogram.

2. The liquid chromatograph with a multi-channel chromatographic column according to claim 1, characterized in that: The main flow channel and the secondary flow channel are both opened on one side of the chromatographic column close to the output end; The main flow channels and the secondary flow channels are of the same number and are staggered with each other along the axial direction; The main flow channel and the secondary flow channel both extend radially and expand toward both sides; The area occupied by the main flow channel is smaller than the area occupied by the secondary flow channel.

3. The liquid chromatograph with a multi-channel chromatographic column according to claim 1, characterized in that: The adjustment component comprises: A switching tube, which can selectively communicate with the main flow channel / the secondary flow channel; The switching sleeve is rotatably sleeved on the outside of the chromatographic column and fixedly connected to the switching tube, and is used to adjust the connection state between the switching tube and the main flow channel / secondary flow channel.

4. The liquid chromatograph with a multi-channel chromatographic column according to claim 1, characterized in that: The chromatographic column also includes: A column tube, one end of which is connected to a liquid inlet tube, the other end of which is connected to the liquid inlet end of the chromatographic column, and the other end of which is connected to a liquid outlet tube, the other end of which is connected to the liquid outlet end of the chromatographic column; The filter part is placed in the column tube and fixedly connected to both ends of the column tube, and is used to filter impurities in the liquid and the sample.

5. The liquid chromatograph with a multi-channel chromatographic column according to claim 4, characterized in that: The switching component comprises: An upper liquid tube and a lower liquid tube, both of which are placed outside the column tube; A first valve body, which is used to control the on-off of the liquid inlet pipe; A second valve body, which is used to control the on-off of the upper liquid pipe; A third valve body, which is used to control the on-off of the liquid outlet pipe; The fourth valve body is used to control the opening and closing of the lower liquid pipe.

6. The liquid chromatograph with a multi-channel chromatographic column according to claim 5, characterized in that: When the first valve body is connected to the third valve body, the sample and the liquid pass through the liquid inlet tube, the column tube, and the liquid outlet tube in sequence to separate the sample; When the second valve body is connected to the fourth valve body, the liquid passes through the liquid inlet pipe, the upper liquid pipe, the column pipe, the lower liquid pipe and the liquid outlet pipe in sequence to perform backwash cleaning of the chromatographic column.

7. The liquid chromatograph with a multi-channel chromatographic column according to claim 1, characterized in that: The groove depths in the central areas of the main flow channel and the secondary flow channel are greater than the groove depths in the edge areas thereof, thereby suppressing the central flow velocity and reducing the flow resistance.

8. The liquid chromatograph with a multi-channel chromatographic column according to claim 4, characterized in that: The end of the main flow channel extends to the inner side of the column tube, and the end of the secondary flow channel is close to the inner side of the column tube.

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