An apparatus and method for detecting and analyzing the immune microenvironment of solid tumors

Abstract

The present invention relates to the technical field of tumor immune microenvironment detection and analysis, and discloses an apparatus and method for detecting and analyzing the immune microenvironment of solid tumors. The apparatus for detecting and analyzing the immune microenvironment of solid tumors includes: a detection mechanism: used to contact with a tumor sample or puncture a part of the tumor sample and upload data for operators to analyze; a transportation and cleaning mechanism integrating transportation, cleaning and adjustment functions: cleaning both sides of the sample synchronously during transportation and adapting to different sample sizes; a clamping and turning mechanism: clamping the sample and turning it to position to expose the cleaning surface; a collection component: detachably recovering the shed impurities; a wiping component: used to clean the detection area of the detection mechanism after detection to prevent residual substances from affecting subsequent detection results. The transportation and cleaning mechanism of the present invention can automatically clean the impurities outside the sample while transporting the tumor sample, and can also adjust according to samples of different sizes, improving the accuracy of subsequent detection.

Description

Technical Field

[0001] The present invention relates to the technical field of tumor immune microenvironment detection and analysis, and specifically to a device and method for detecting and analyzing the immune microenvironment of solid tumors. Background Art

[0002] The detection of the tumor immune microenvironment is of great significance in modern medical research and clinical diagnosis. With the progress of science and technology, in-depth analysis of the tumor immune microenvironment can help researchers better understand the occurrence mechanism of tumors and their interaction with the immune system, thus providing strong support for the early diagnosis and personalized treatment of tumors. To ensure the accuracy of the detection results, a series of operations such as precise transportation, cleaning, clamping, and probe cleaning of tumor samples are required.

[0003] In the prior art, the transportation and cleaning of tumor samples are usually carried out separately. Most devices move samples from one area to another through a separate transportation system, and the cleaning work relies on another set of devices or manual operations. Such a design can complete the basic functions, but often has the problems of cumbersome operation processes and low efficiency. Moreover, during the transportation of tumor samples, impurities are likely to adhere to the outside of the samples, thus affecting the subsequent detection results. To solve this problem, some devices have added cleaning components to remove impurities and improve the cleanliness of the samples. Nevertheless, the effects of these technologies are still limited to single operations and cannot achieve efficient integration.

[0004] However, there are still some deficiencies in the prior art; firstly, the transportation and cleaning operations in the prior art are carried out separately, resulting in cumbersome equipment and complex operations. For the detection of the tumor immune microenvironment that requires high precision and efficiency, the separate operation processes not only increase the manual burden but also easily lead to sample contamination due to improper operations; secondly, the existing clamping systems generally can only provide simple clamping, lacking flipping and adjusting functions, and cannot accurately control the position and angle of tumor samples, easily causing incomplete exposure of the detection area; thirdly, most of the prior art ignores the cleaning of the residual substances on the periphery of the detection probes, which means that there may be a risk of cross-contamination after each use, resulting in inaccurate subsequent detections. Summary of the Invention

[0005] Aiming at the deficiencies of the prior art, the present invention provides a device and method for detecting and analyzing the immune microenvironment of solid tumors, which solves the problems of cumbersome, low-efficiency separate operations of tumor sample transportation, cleaning, clamping, and probe cleaning in the prior art and is prone to sample contamination.

[0006] To achieve the above objectives, the present invention is realized through the following technical solutions: A device for detecting and analyzing the immune microenvironment of solid tumors, comprising:

[0007] A detection mechanism, which is used to contact with a tumor sample or puncture part of the tumor sample and then upload data for operators to analyze;

[0008] A transportation and cleaning mechanism, which is arranged on the upper side of the detection mechanism. The transportation and cleaning mechanism includes a transportation component, a cleaning component and an adjustment component. The transportation component is used to provide support and storage for the tumor sample and transport the tumor sample to the detection area. The cleaning component is used to sweep both sides of the tumor sample during the transportation of the tumor sample, so that the impurities adsorbed on the outside of the tumor sample fall off. The adjustment component is used to adjust the space for storing the tumor sample to accommodate tumor samples of different sizes;

[0009] A clamping and turning mechanism, which is arranged on the upper side of the detection mechanism, is used to clamp and move the tumor sample to the detection area, and turn the tumor sample during the movement, so that the area of the tumor sample to be cleaned is exposed in the detection area;

[0010] A collection component, which is arranged at the bottom of the detection mechanism, is used to collect the impurities falling off the outside of the tumor sample and extract them later for unified cleaning;

[0011] A wiping component, which is arranged inside the detection mechanism, is used to clean the detection area of the detection mechanism after detection to prevent residual substances from affecting subsequent detection results.

[0012] Preferably, the detection mechanism includes a support frame. A frame is fixedly connected to the upper side of the support frame. A cylinder is fixedly connected to the top of the frame. The output end of the cylinder is fixedly connected to a detection probe.

[0013] Preferably, the transportation component includes a first hydraulic cylinder. The first hydraulic cylinder is fixedly connected to the upper side of the support frame. The output end of the first hydraulic cylinder is fixedly connected to a push plate. Two connecting plates are arranged on the other side of the push plate.

[0014] Preferably, the cleaning component includes two groups of brush rollers. The two groups of brush rollers are respectively rotatably connected to the middle parts of the two connecting plates. A connecting component is fixedly connected to the bottom of the brush roller. The connecting component is composed of a tensioning sprocket and a tensioning chain and is used to rotate any group of brush rollers simultaneously. A gear is fixedly connected to the upper side of the brush roller. An L-shaped connecting block is fixedly connected to the outside of the connecting plate. Two limit blocks are slidably connected to the inner bottom of the frame. A rack is fixedly connected to the bottom of the limit block. The gear and the rack are meshed with each other. The side of the L-shaped connecting block away from the connecting plate is T-shaped. The side of the L-shaped connecting block away from the connecting plate is slidably connected to the middle of the rack.

[0015] Preferably, the adjusting assembly includes a bidirectional lead screw rotatably connected to the middle of the push plate. Two nut seats are threadedly connected to the outer periphery of the bidirectional lead screw, and the connecting plate is fixedly connected to the outside of the nut seat.

[0016] Preferably, the clamping and steering mechanism includes a second hydraulic cylinder fixedly connected to the upper side of the support frame. The output end of the second hydraulic cylinder is rotatably connected to a connecting rod, and a convex block is fixedly connected to the outside of the connecting rod. An arc-shaped groove is formed inside the frame, and the convex block is slidably connected to the middle of the arc-shaped groove. One end of the connecting rod away from the second hydraulic cylinder is fixedly connected to a machine shell, and a connecting disc is fixedly connected to the other side of the machine shell. A motor is fixedly connected to the inside of the machine shell, and the output end of the motor is fixedly connected to a turntable rotatably connected to the middle of the connecting disc. An arc-shaped plate is rotatably connected to the outside of the turntable, and a slider is rotatably connected to the other side of the arc-shaped plate. A clamping plate is fixedly connected to the other side of the slider.

[0017] Preferably, the collecting assembly includes two L-shaped support blocks fixedly connected to the bottom of the support frame. A collecting box is slidably connected between the two L-shaped support blocks.

[0018] Preferably, the wiping assembly includes a sleeve fixedly connected to the inner bottom of the frame. Multiple groups of bristles are fixedly connected to the inside of the sleeve, and the bristles are in contact with the detection probe.

[0019] Preferably, a chute is formed in the middle of the connecting disc, and the slider is slidably connected to the middle of the chute.

[0020] The present invention also provides a method for using a detection and analysis device for the immune microenvironment of solid tumors, including the following steps:

[0021] Step 1: Rotate the bidirectional lead screw forward or backward to make the nut seats drive the two connecting plates to approach or move away from each other. After adjusting to the size where the current tumor sample can be placed, place the tumor sample between the two connecting plates. At this time, drive the first hydraulic cylinder to push the push plate and the connecting plate to move. When the push plate moves, through the limitation of the limiting block and within the sliding range provided by the L-shaped connecting block, the meshing between the gear and the rack can be maintained when the connecting plate moves, and the gear can drive the brush roller to rotate. When one of the brush rollers rotates, through the design of the tensioning sprocket and the tensioning chain, multiple brush rollers can rotate simultaneously, so as to clean the impurities attached to both sides of the tumor sample.

[0022] Step 2: After the transportation and cleaning mechanism cleans and transports the tumor sample into the device, drive the second hydraulic cylinder to make the connecting rod drive the clamping part to move. While moving, through the limit of the arc-shaped groove and the cooperation of the convex block, the connecting rod can be flipped, so that the clamping part is in a vertical state, and after flipping, it moves to the tumor sample area. At this time, drive the rotary table to rotate through the drive motor. During the rotation process, drive the arc-shaped plate to displace. At the same time, due to the limit of the slider, the arc-shaped plate can drive the two clamping plates to move in parallel and clamp the tumor sample. After clamping, retract the connecting rod through the second hydraulic cylinder, and during the retraction process, make the clamping part flip and be in a parallel state. At this time, it is convenient for the detection probe to detect the tumor sample;

[0023] Step 3: During detection, drive the detection probe to descend through the brush bristles and contact or puncture into the tumor sample for collection and detection through the drive cylinder. After the detection is completed, drive the cylinder to make the detection probe rise into the sleeve and be blocked by the brush bristles, so that the substances remaining on the outer periphery of the detection probe can be removed;

[0024] Step 4: When the collected impurities need to be cleaned, pull the collection box and under the limit of the L-shaped support block, the collection box can be pulled out in parallel and the impurities it collects can be cleaned. Under the positioning of the L-shaped support block, the collection box can be quickly put into use state.

[0025] The present invention provides a detection and analysis device and method for the immune microenvironment of solid tumors. It has the following beneficial effects:

[0026] 1. The transportation and cleaning mechanism of the present invention combines the functions of transportation, cleaning and adjustment. It can automatically clean the impurities outside the sample while transporting the tumor sample, avoiding the influence of impurities on the tumor detection effect. At the same time, compared with the prior art which can only transport and clean separately, this integrated design saves time and is efficient, directly improving the accuracy of subsequent detection.

[0027] 2. The design of the clamping and turning mechanism can clamp and flip the tumor sample, and turn the area after cleaning the tumor sample to the bottom of the detection probe, making the sample more accurate during detection.

[0028] 3. Through the combination of the brush bristles and the sleeve, after each detection, the brush bristles will clean the substances on the outer periphery of the detection probe, ensuring no cross-contamination and preventing impurities from remaining on the outer periphery of the detection probe, further improving the accuracy of the detection result. Description of the Drawings

[0029] Figure 1 is a three-dimensional view of the present invention;

[0030] Figure 2 is a schematic cross-sectional view of the framework of the present invention;

[0031] Figure 3Schematic structural diagram of the wiping component of the present invention;

[0032] Figure 4 Schematic cross-sectional view of the connecting plate of the present invention;

[0033] Figure 5 Schematic structural diagram of the arc-shaped groove of the present invention;

[0034] Figure 6 Schematic structural diagram of the bump of the present invention;

[0035] Figure 7 Schematic structural diagram of the arc-shaped plate of the present invention;

[0036] Figure 8 Schematic structural diagram of the collection component of the present invention.

[0037] Among them, 10, detection mechanism; 101, support frame; 102, frame; 103, cylinder; 104, detection probe; 20, transportation and cleaning mechanism; 201, first hydraulic cylinder; 202, push plate; 203, connecting plate; 204, brush roller; 205, tensioning sprocket; 206, tensioning chain; 207, gear; 208, L-shaped connecting block; 209, rack; 210, limit block; 211, bidirectional lead screw; 212, nut seat; 30, clamping and steering mechanism; 301, second hydraulic cylinder; 302, connecting rod; 303, bump; 304, arc-shaped groove; 305, housing; 306, connecting disk; 307, motor; 308, turntable; 309, arc-shaped plate; 310, slider; 311, clamping plate; 40, collection component; 401, L-shaped support block; 402, collection box; 50, wiping component; 501, sleeve; 502, bristles. Detailed implementation manners

[0038] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the specification of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0039] Please refer to Figures 1 - 8 , an entity tumor immune microenvironment detection and analysis device provided by an embodiment of the present invention includes:

[0040] A detection mechanism 10 for contacting a tumor sample or puncturing a part of the tumor sample and then uploading data for an operator to analyze;

[0041] The transportation and cleaning mechanism 20 is arranged above the detection mechanism 10. The transportation and cleaning mechanism 20 includes a transportation component, a cleaning component and an adjustment component. The transportation component is used to provide support and storage for tumor samples and transport the tumor samples to the detection area. The cleaning component is used to sweep both sides of the tumor sample during the transportation of the tumor sample, so that the impurities adsorbed on the outside of the tumor sample fall off. The adjustment component is used to adjust the space for storing tumor samples to accommodate tumor samples of different sizes;

[0042] The clamping and turning mechanism 30 is arranged above the detection mechanism 10 and is used to clamp and move the tumor sample to the detection area and turn the tumor sample during the movement, so that the area of the tumor sample to be cleaned is exposed in the detection area;

[0043] The collection component 40 is arranged at the bottom of the detection mechanism 10 and is used to collect the impurities falling off the outside of the tumor sample and extract them for unified cleaning later;

[0044] The wiping component 50 is arranged inside the detection mechanism 10 and is used to clean the detection area of the detection mechanism 10 after detection to prevent residual substances from affecting subsequent detection results.

[0045] Please refer to Figure 1 、 Figure 2 and Figure 5 In a preferred embodiment of the present invention, the detection mechanism 10 includes a support frame 101. A frame 102 is fixedly connected to the upper side of the support frame 101. A cylinder 103 is fixedly connected to the top of the frame 102. The output end of the cylinder 103 is fixedly connected to a detection probe 104. The support frame 101 is used to support the device for the convenience of operation by the operator. The cylinder 103 is used to adjust the height of the detection probe 104. When the cylinder 103 is driven, the detection probe 104 moves downward and contacts the tumor sample to complete the detection operation. The detection operation of the detection probe 104 is prior art and will not be elaborated herein.

[0046] Please refer to Figure 1 、 Figure 2 and Figure 4 In a preferred embodiment of the present invention, the transportation component includes a first hydraulic cylinder 201. The first hydraulic cylinder 201 is fixedly connected to the upper side of the support frame 101. The output end of the first hydraulic cylinder 201 is fixedly connected to a push plate 202. Two connecting plates 203 are arranged on the other side of the push plate 202. The connecting plates 203 are used to store and support the tumor sample. At the same time, the first hydraulic cylinder 201 is used to drive the movement of the push plate 202 and the connecting plates 203, so as to transport the tumor to the device for waiting for the detection operation.

[0047] Please refer to Figure 4 and Figure 5, in a preferred embodiment of the present invention, the cleaning component includes two groups of brush rollers 204. The two groups of brush rollers 204 are respectively rotatably connected to the middle parts of two connecting plates 203. A connecting component is fixedly connected to the bottom of the brush roller 204. The connecting component is composed of a tensioning sprocket 205 and a tensioning chain 206, and is used to rotate any group of brush rollers 204 simultaneously. Through the setting of the connecting component, when one of the brush rollers 204 rotates, it can drive all the brush rollers 204 in this group to rotate, so as to complete the cleaning of the tumor sample. At the same time, the connecting component is a prior art and will not be elaborated too much in the text. A gear 207 is fixedly connected to the upper side of the brush roller 204. An L-shaped connecting block 208 is fixedly connected to the outside of the connecting plate 203. Two limiting blocks 210 are slidably connected to the inner bottom of the frame 102. A rack 209 is fixedly connected to the bottom of the limiting block 210. The gear 207 and the rack 209 are meshed with each other. Through the setting of the limiting block 210, the position of the rack 209 can be adjusted, and the connection with the gear 207 can be maintained, so as to ensure that while transporting the tumor, the gear 207 drives the brush roller 204 to rotate, thereby completing the cleaning operation on the outside of the tumor sample. One side of the L-shaped connecting block 208 away from the connecting plate 203 is set as a T shape, and the side of the L-shaped connecting block 208 away from the connecting plate 203 is slidably connected to the middle part of the rack 209. Through the setting of the L-shaped connecting block 208 and the T-shaped setting, when the connecting plate 203 is adjusted, it can drive the rack 209 to be adjusted simultaneously.

[0048] Please refer to Figure 4 , in a preferred embodiment of the present invention, the adjusting component includes a bidirectional lead screw 211. The bidirectional lead screw 211 is rotatably connected to the middle part of the push plate 202. Two nut seats 212 are threadedly connected to the outer periphery of the bidirectional lead screw 211. The connecting plate 203 is fixedly connected to the outside of the nut seat 212. By rotating the bidirectional lead screw 211, the nut seat 212 can drive the connecting plate 203 to move closer to or away from each other, so as to adjust the distance between the two connecting plates 203 according to the size of the tumor sample, so that the cleaning component can clean the outside of the tumor sample of any size.

[0049] Please refer to Figures 5 - 7, in a preferred embodiment of the present invention, the clamping steering mechanism 30 includes a second hydraulic cylinder 301. The second hydraulic cylinder 301 is fixedly connected to the upper side of the support frame 101. The output end of the second hydraulic cylinder 301 is rotatably connected to a connecting rod 302. A convex block 303 is fixedly connected to the outside of the connecting rod 302. An arc-shaped groove 304 is formed inside the frame 102. The convex block 303 is slidably connected to the middle of the arc-shaped groove 304. One end of the connecting rod 302 away from the second hydraulic cylinder 301 is fixedly connected to a machine shell 305. A connecting disk 306 is fixedly connected to the other side of the machine shell 305. A motor 307 is fixedly connected to the inside of the machine shell 305. The output end of the motor 307 is fixedly connected to a turntable 308. The turntable 308 is rotatably connected to the middle of the connecting disk 306. An arc-shaped plate 309 is rotatably connected to the outside of the turntable 308. A slider 310 is rotatably connected to the other side of the arc-shaped plate 309. A clamping plate 311 is fixedly connected to the other side of the slider 310. Through the rotational design of the connecting rod 302, it is allowed that the connecting rod 302 rotates when driven by the second hydraulic cylinder 301. Under the limitation of the arc-shaped groove 304, when the second hydraulic cylinder 301 drives the connecting rod 302 to move forward, the connecting rod 302 is flipped by extrusion, so that the two clamping plates 311 can be changed from the left and right sides to the up and down sides for clamping. At this time, the driving motor 307 makes the turntable 308 rotate. While it rotates, the arc-shaped plate 309 can only drive the clamping plate 311 to move parallelly through the limitation of the slider 310, so as to clamp the tumor sample. And when the second hydraulic cylinder 301 drives the connecting rod 302 to reset after clamping, the connecting rod 302 can be reversely flipped and reset, so that the side for cleaning the tumor sample is perpendicular to the bottom of the detection probe 104, thereby improving the detection accuracy.

[0050] Please refer to Figure 1 and Figure 8 , in a preferred embodiment of the present invention, the collection assembly 40 includes two L-shaped support blocks 401. The L-shaped support blocks 401 are fixedly connected to the bottom of the support frame 101. A collection box 402 is slidably connected between the two L-shaped support blocks 401. The provided L-shaped support blocks 401 can limit the collection box 402 and at the same time provide support for the collection box 402, prevent the collection box 402 from falling and provide a slidable space for the collection box 402. By sliding the collection box 402, the collection box 402 can be quickly assembled or disassembled and cleaned.

[0051] Please refer to Figure 2 and Figure 3 , in a preferred embodiment of the present invention, the wiping assembly 50 includes a sleeve 501. The sleeve 501 is fixedly connected to the inner bottom of the frame 102. A plurality of groups of bristles 502 are fixedly connected to the inside of the sleeve 501. The bristles 502 are in contact with the detection probe 104. The provided bristles 502 can remove the substances remaining on the outer periphery of the detection probe 104 when the detection probe 104 is retracted, thereby improving the accuracy of the next detection.

[0052] Please refer to Figure 6 In a preferred embodiment of the present invention, a chute is provided in the middle of the connecting plate 306, and the slider 310 is slidably connected to the middle of the chute. By providing the chute, the slider 310 can be limited, so that the slider 310 only drives the clamping plate 311 to move parallelly, avoiding the inclination or deviation of the clamping plate 311, and improving the clamping accuracy and effect.

[0053] The present invention also provides a method for using an apparatus for detecting and analyzing the immune microenvironment of solid tumors, including the following steps:

[0054] Step 1: Rotate the bidirectional lead screw 211 forward or backward to make the nut seat 212 drive the two connecting plates 203 to approach or move away from each other. After adjusting to the size where the current tumor sample can be placed, place the tumor sample between the two connecting plates 203. At this time, drive the hydraulic cylinder 1 201 to move the push plate 202 and the connecting plate 203. When the push plate 202 moves, through the limitation of the limiting block 210 and within the sliding range provided by the L-shaped connecting block 208, the meshing between the gear 207 and the rack 209 can be maintained when the connecting plate 203 moves, and the gear 207 drives the brush roller 204 to rotate. When one of the brush rollers 204 rotates, through the design of the tensioning sprocket 205 and the tensioning chain 206, multiple brush rollers 204 can rotate simultaneously, so as to clean the impurities attached to both sides of the tumor sample;

[0055] Step 2: When the transportation and cleaning mechanism 20 cleans and transports the tumor sample to the inside of the apparatus, drive the hydraulic cylinder 2 301 to make the connecting rod 302 drive the clamping part to move. During the movement, through the limitation of the arc-shaped groove 304 and the cooperation of the convex block 303, the connecting rod 302 can be flipped, so that the clamping part is in a vertical state, and after flipping, it moves to the tumor sample area. At this time, drive the driving motor 307 to rotate the turntable 308. During the rotation process, the arc-shaped plate 309 is displaced. At the same time, due to the limitation of the slider 310, the arc-shaped plate 309 can drive the two clamping plates 311 to move parallelly and clamp the tumor sample. After clamping, drive the hydraulic cylinder 2 301 to retract the connecting rod 302, and during the retraction process, the clamping part is flipped and in a parallel state. At this time, it is convenient for the detection probe 104 to detect the tumor sample;

[0056] Step 3: During detection, drive the driving cylinder 103 to lower the detection probe 104 to pass through the bristles 502 and contact or puncture into the tumor sample for collection and detection. After the detection is completed, drive the driving cylinder 103 to raise the detection probe 104 into the sleeve 501 and be blocked by the bristles 502, so as to remove the substances remaining on the outer periphery of the detection probe 104;

[0057] Step Four: When the collected impurities need to be cleaned, by pulling the collection box 402, under the limit of the L-shaped support block 401, the collection box 402 can be drawn out in parallel to clean the impurities it has collected, and under the positioning of the L-shaped support block 401, the collection box 402 can be quickly put into use state.

[0058] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An apparatus for detecting and analyzing the immune microenvironment of solid tumors, characterized in that, Including: A detection mechanism (10), the detection mechanism (10) includes a support frame (101), a frame (102) is fixedly connected to the upper side of the support frame (101), a cylinder (103) is fixedly connected to the top of the frame (102), and a detection probe (104) is fixedly connected to the output end of the cylinder (103); A transportation and cleaning mechanism (20), the transportation and cleaning mechanism (20) includes a transportation component, a cleaning component and an adjustment component. The transportation component includes a first hydraulic cylinder (201), the first hydraulic cylinder (201) is fixedly connected to the upper side of the support frame (101), a push plate (202) is fixedly connected to the output end of the first hydraulic cylinder (201), and two connecting plates (203) are arranged on the other side of the push plate (202); The cleaning component includes two groups of brush rollers (204), the two groups of brush rollers (204) are respectively rotatably connected to the middle parts of the two connecting plates (203), a connecting component is fixedly connected to the bottom of the brush roller (204), and the connecting component is composed of a tensioning sprocket (205) and a tensioning chain (206) for simultaneously rotating any group of brush rollers (204). A gear (207) is fixedly connected to the upper side of the brush roller (204), an L-shaped connecting block (208) is fixedly connected to the outside of the connecting plate (203), two limit blocks (210) are slidably connected to the inner bottom of the frame (102), a rack (209) is fixedly connected to the bottom of the limit block (210), the gear (207) and the rack (209) are meshed with each other, and the side of the L-shaped connecting block (208) away from the connecting plate (203) is T-shaped, and the side of the L-shaped connecting block (208) away from the connecting plate (203) is slidably connected to the middle of the rack (209); The adjustment component includes a bidirectional lead screw (211), the bidirectional lead screw (211) is rotatably connected to the middle of the push plate (202), two nut seats (212) are threadedly connected to the outer circumference of the bidirectional lead screw (211), and the connecting plate (203) is fixedly connected to the outside of the nut seat (212); Clamping steering mechanism (30), the clamping steering mechanism (30) includes a second hydraulic cylinder (301), the second hydraulic cylinder (301) is fixedly connected to the upper side of the support frame (101), the output end of the second hydraulic cylinder (301) is rotatably connected to a connecting rod (302), a convex block (303) is fixedly connected to the outer side of the connecting rod (302), an arc-shaped groove (304) is formed inside the frame (102), the convex block (303) is slidably connected to the middle of the arc-shaped groove (304), one end of the connecting rod (302) away from the second hydraulic cylinder (301) is fixedly connected to a housing (305), a connecting disk (306) is fixedly connected to the other side of the housing (305), a motor (307) is fixedly connected to the inside of the housing (305), an output end of the motor (307) is fixedly connected to a turntable (308), the turntable (308) is rotatably connected to the middle of the connecting disk (306), an arc-shaped plate (309) is rotatably connected to the outer side of the turntable (308), a slider (310) is rotatably connected to the other side of the arc-shaped plate (309), and a clamping plate (311) is fixedly connected to the other side of the slider (310); Collection assembly (40), the collection assembly (40) includes two L-shaped support blocks (401), the L-shaped support blocks (401) are fixedly connected to the bottom of the support frame (101), and a collection box (402) is slidably connected between the two L-shaped support blocks (401); Wiping assembly (50), the wiping assembly (50) includes a sleeve (501), the sleeve (501) is fixedly connected to the inner bottom of the frame (102), multiple groups of bristles (502) are fixedly connected to the inside of the sleeve (501), and the bristles (502) are in contact with the detection probe (104).

2. The detection and analysis device for the immune microenvironment of solid tumors according to claim 1, wherein A chute is formed in the middle of the connecting disk (306), and the slider (310) is slidably connected to the middle of the chute.

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