Insecticidal pesticide composition for preventing and treating Chinese herbaceous peony spider mites

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to an insecticidal pesticide composition for preventing and treating spider mites of paeonia lactiflora. The invention relates to an insecticidal pesticide composition for preventing and treating spider mites of Chinese herbaceous peony. The insecticidal pesticide composition is prepared by compounding afidopyropen and silafluofen or cyflumetofen according to a mass ratio of (1 to 100): (100 to 1). The compounded combination of the insecticidal pesticide composition shows a combined synergistic effect on the peony pest mites, namely tetranychus cinnabarinus, can improve the control effect on the peony pest mites, reduces the use amount of pesticides and pesticide residues on peony plants, and is beneficial to green and healthy development of the peony industry.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticides, and particularly relates to an insecticidal pesticide composition for controlling Tetranychus ludeni Zacher. Background Art

[0002] Paeonia lactiflora Pall. is a perennial herbaceous plant of the genus Paeonia in the family Ranunculaceae. It contains various active substances in its roots, stems, leaves, flowers and seeds, and has high medicinal value. The main bioactive components in the roots of Paeonia lactiflora Pall. are monoterpene glycosides such as paeoniflorin and its homologues. Its pharmacological activities mainly include protecting the central nervous and cardiovascular systems, protecting the liver from hepatitis, anti-tumor, analgesic and relieving rheumatoid arthritis. In addition, the stems and leaves of Paeonia lactiflora Pall. contain isomers of oleanolic acid and ursolic acid, which have anti-inflammatory, anti-diabetic, anti-tumor, anti-inflammatory and liver-protecting effects.

[0003] Tetranychus cinnabarinus (Boisduval), also known as the two-spotted spider mite, is a cosmopolitan pest mite that can damage a variety of flowers such as cherry blossoms, peonies, roses, Magnolia denudata Desr. and asparagus ferns. On peonies, Tetranychus cinnabarinus (Boisduval) mainly sucks the sap on the back of the host leaves with adult mites and nymphs, causing white dots on the leaf surface. As the damage intensifies, the leaves turn yellow and wither and fall off. In addition, the massive sucking of Tetranychus cinnabarinus (Boisduval) will affect the photosynthesis and nutrient absorption of peonies, resulting in poor growth of the plants. Moreover, Tetranychus cinnabarinus (Boisduval) may transmit other pathogens during the sucking process, further endangering the healthy growth of peonies.

[0004] Afidopyropen is a microbial insecticide obtained by fermenting and processing the fungus Aspergillus. It has a unique pyropene chemical structure and a novel mechanism of action, and is considered to be the first member of Group 9D in the classification of insecticide action mechanisms. Afidopyropen belongs to a channel regulator of the vanilloid receptor subfamily of chordotonal organs. By interfering with the regulation of the transient receptor potential vanilloid channel complex in target insects, it causes insects to lose their sense of gravity, balance, sound, position and movement, lose coordination and sense of direction, and then be unable to feed, lose water, and ultimately die of starvation. It is suitable for controlling pests on economic crops, field crops and ornamental plants, and can be used for foliar treatment, seed treatment or soil treatment.

[0005] In production, the acaricide can be used to control Tetranychus cinnabarinus on peonies. However, if a pesticide is used for a long time, it will inevitably lead to the emergence of pest resistance (for example: studies have shown that Bemisia tabaci can participate in the resistance evolution to the acaricide through the overexpression of P450 genes CYP6DW3 and CYP4C64), resulting in problems such as reduced drug efficacy. In terms of resistance management, combining the acaricide with other agents and screening compound formulations with synergistic effects can improve the control effect on the target and overcome and delay the problem of resistance. The prior art has disclosed that the acaricide can be compounded with abamectin, tolfenpyrad or ethiprole, etc. However, the mechanism of the synergistic effect produced by pesticide compounding is not clear, and not all compoundings of the acaricide with any insecticidal active ingredient can produce a synergistic effect. Sometimes, an antagonistic effect may even occur.

[0006] So far, there has been no relevant report on the compounding of the acaricide with silafluofen or cyflumetofen. Summary of the Invention

[0007] The purpose of the present invention is to provide an insecticidal pesticide composition for controlling peony leaf mites to solve the problems existing when using the acaricide alone to control peony pest mites.

[0008] To achieve the above purpose, the present invention provides the following technical solutions:

[0009] An insecticidal pesticide composition for controlling peony leaf mites, which is compounded by the acaricide and silafluofen or cyflumetofen in a mass ratio of 1-100:100-1.

[0010] Further, the compounding mass ratio of the acaricide and silafluofen is 1-5:25-1.

[0011] Further, the compounding mass ratio of the acaricide and cyflumetofen is 1-30:30-1.

[0012] An insecticide including the above insecticidal pesticide composition, in the insecticide, the mass of the pesticide composition accounts for 0.1-55% of the total mass of the insecticide, and the balance is auxiliary components allowed to be added in pesticide science.

[0013] Further, the mass of the pesticide composition accounts for 1-35% of the total mass of the insecticide.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] (1) The insecticidal pesticide composition of the present invention is composed of the combination and compounding of cyantraniliprole with silafluofen or cyflumetofen, which can reduce the risk of drug resistance, overcome and delay the problem of cyantraniliprole drug resistance, and is of great significance for the scientific management of cyantraniliprole drug resistance and ensuring its long-term effective application.

[0016] (2) The compound combination of the insecticidal pesticide composition of the present invention shows a synergistic effect on the peony pest mite Tetranychus cinnabarinus, can improve the control effect on the peony pest mite, reduce the amount of pesticide used, and the pesticide residue on the peony plants, which helps the green and healthy development of the peony industry. Specific embodiments

[0017] The technical solutions of the present invention will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of them. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative work belong to the scope of protection of the present invention.

[0018] Example: Indoor bioassay

[0019] 1. Test pest mites

[0020] Collect Tetranychus cinnabarinus on peony plants and continuously raise them indoors until the 8th generation. Select female adult mites with consistent physiological states as test pest mites.

[0021] 2. Rearing conditions

[0022] Temperature: 25 ± 1 °C; humidity 65%; light: 14L / 10D.

[0023] 2. Test agents

[0024] 92.5% cyantraniliprole technical, 93% silafluofen technical, 98% cyflumetofen technical. The above agents are all commercially available.

[0025] Accurately weigh the test agents with an electronic analytical balance, completely dissolve them with a solvent, and then dilute them with 0.1% (v / v) TritonX-100 aqueous solution to prepare single-agent mother liquors. According to the results of the preliminary test, set multiple groups of formulated mixtures, respectively take appropriate volumes of the single-agent mother liquors and the formulated mixtures, and dilute them with 0.1% (v / v) Triton X-100 aqueous solution to prepare 5 series of mass concentrations.

[0026] 3. Test method: (Refer to "Pesticide Indoor Bioassay Guidelines Insecticides Part 12: Slide Immersion Method for Tetranychidae")

[0027] Cut double-sided tape of appropriate length and paste it onto one end of the glass slide. Stick the back of the test mite pests onto the double-sided tape, without sticking to the mite legs, mite whiskers and mouthparts. Stick two rows of test mite pests on each glass slide, with 20 mites in each row, for a total of 40 mites. Place the glass slide in a petri dish padded with a wet sponge. After 2 hours, examine it under a binocular stereomicroscope, remove the dead and injured individuals and make up to 40 mites on each glass slide. Then immerse the glass slide completely in the liquid medicine for 5 s and take it out, dry the liquid medicine around the glass slide and the mites with filter paper, and use the treatment with 0.1% (v / v) Triton X-100 aqueous solution as the blank control. Each treatment has 3 replicates. Place it under the original feeding conditions for feeding and observation. After 48 hours, investigate the survival of the test mite pests under a binocular stereomicroscope, record the total number of insects and the number of dead insects in each treatment, and calculate the corrected mortality of each treatment. Among them, when gently touching the mite body with a writing brush and the mite legs do not move, it is regarded as dead.

[0028]

[0029] In the above formula: P - mortality rate, unit: %; K - number of dead insects; N - total number of insects in the treatment.

[0030]

[0031] In the above formula: P 1 -- corrected mortality rate, unit: %; P t -- treatment mortality rate, unit: %; P 0 -- blank control mortality rate, unit: %.

[0032] 4. Data analysis

[0033] Conduct a regression analysis on the logarithm of the concentration of each treatment agent and the probit value of the corrected mortality rate, and calculate the LC 50 of each treatment agent. Calculate the co-toxicity coefficient (CTC value) of the mixture by the Sun Yunpei method.

[0034] Actual toxicity index (ATI) = (LC 50 of the standard agent / LC 50 of the test agent) × 100

[0035] Theoretical toxicity index (TTI) = toxicity index of agent A × percentage content of agent A in the mixture + toxicity index of agent B × percentage content of agent B in the mixture

[0036] Co-toxicity coefficient (CTC) = [actual toxicity index (ATI) of the mixture / theoretical toxicity index (TTI) of the mixture] × 100

[0037] 5. Efficacy evaluation

[0038] The synergistic effect of the drugs was evaluated according to the co-toxicity coefficient: CTC≤80 was antagonistic effect, 80<CTC<120 was additive effect, and CTC≥120 was synergistic effect. The results are shown in Table 1-2.

[0039] Table 1 Test results of the combination of cypermethrin and flutosan against Tetranychus cinnabarinus (48h)

[0040]

[0041]

[0042] As shown in Table 1, when cyproconazole and silafluthrin are compounded in the mass ratio range of 1-5:25-1, the co-toxicity coefficient to Tetranychus cinnabarinus of peony (48h) is greater than 120, that is, the biological activity of cyproconazole and silafluthrin against Tetranychus cinnabarinus of peony shows a synergistic effect.

[0043] Table 2 Test results of the combination of cyproconazole and cyfluthrin against Tetranychus cinnabarinus (48h)

[0044]

[0045] As shown in Table 2, when diflubenzuron and cyflumetofen are compounded in the mass ratio range of 1-30:30-1, the co-toxicity coefficients against Tetranychus cinnabarinus of peony (48h) are all greater than 120, that is, the biological activity of diflubenzuron and cyflumetofen against Tetranychus cinnabarinus of peony shows a synergistic effect.

[0046] In summary, the insecticide pesticide composition of the present invention is compounded by combining cypermethrin with flutolan or cyfluthrin, which can reduce the risk of drug resistance, overcome and delay the problem of cypermethrin resistance, and has important significance for the scientific management of cypermethrin resistance and ensuring its long-term effective application. The compound combination of the insecticide pesticide composition of the present invention shows a combined synergistic effect on the peony pest mite Tetranychus cinnabarinus, which can improve the control effect on the peony pest mite, reduce the amount of pesticide used, and the pesticide residues on the peony plant, which is conducive to the green and healthy development of the peony industry.

Claims

1. An insecticide pesticide composition for controlling peony spider mites, characterized in that: The insecticide pesticide composition is prepared by compounding cyprodinil and silafluanid or cyfluthrin in a mass ratio of 1-100:100-1.

2. The insecticide pesticide composition according to claim 1, characterized in that: The compounding mass ratio of cypermethrin to silafluanid is 1-5:25-1.

3. The insecticide pesticide composition according to claim 1, characterized in that: The compounding mass ratio of diprofenopipron to cyflumetofen is 1-30:30-1.

4. An insecticide comprising the insecticide pesticide composition according to any one of claims 1 to 3, characterized in that: In the insecticide, the mass of the pesticide composition accounts for 0.1-55% of the total mass of the insecticide, and the remainder is auxiliary ingredients allowed to be added in pesticide science.

5. The insecticide according to claim 4, characterized in that The mass of the pesticide composition accounts for 1-35% of the total mass of the insecticide.

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