Ca-Si-based bioactive dressing as well as preparation method and application thereof

Abstract

The invention discloses a Ca-Si-based bioactive dressing as well as a preparation method and application thereof. The preparation method comprises the following steps: reacting tetraethoxysilane and calcium nitrate tetrahydrate in an ethanol / ammonia water system, sintering to prepare Ca-Si-based bioactive particles, and reacting the Ca-Si-based bioactive particles with polyethylene glycol and polyvinyl alcohol to prepare the Ca-Si-based bioactive dressing. By introducing Ca-Si-based bioactive particles, proliferation and differentiation of cells can be promoted, so that rapid repair of damaged parts can be accelerated, and the dressing is uniform in component, colorless and transparent, and convenient for observing the repair condition of the damaged parts in real time during use.

Description

Technical Field

[0001] The present invention belongs to the technical field of medical dressings, and particularly relates to a Ca-Si-based bioactive dressing, a preparation method thereof, and an application thereof. Background Art

[0002] The skin is the largest organ of the human body. The delayed wound healing caused by skin injury has become a huge problem faced by the healthcare field, seriously affecting people's health and even threatening life. As an external barrier, the skin can protect internal tissues and organs from external physical damage, mechanical damage, chemical pollution, and invasion by harmful microorganisms. When the skin is damaged, the coordinated action of various cells, signaling factors, and the extracellular matrix is required to restore the integrity of the skin tissue and achieve its functions. When the skin is damaged, most conventional dressings only act as a physical barrier to protect internal tissues from external invasion; some functional dressings can promote wound healing at the damaged site. The occurrence of skin injury is definitely accompanied by the occurrence of inflammation. Therefore, it is necessary to design a dressing material that can regulate the inflammatory immune response and promote injury repair. Due to their unique hydrophilicity, biocompatibility, and skin tissue repair-promoting properties, bioactive nanoparticles gradually have very promising application prospects as effective functional components of wound dressings. Summary of the Invention

[0003] To solve the disadvantages and deficiencies of the prior art, the primary object of the present invention is to provide a preparation method of a Ca-Si-based bioactive dressing.

[0004] Another object of the present invention is to provide a Ca-Si-based bioactive dressing prepared by the above preparation method. This dressing is used for the repair of skin microtrauma or trauma and can promote cell proliferation and spreading.

[0005] Another object of the present invention is to provide an application of the above Ca-Si-based bioactive dressing in the preparation of products for skin wound repair.

[0006] The object of the present invention is achieved by the following technical solutions:

[0007] A preparation method of a Ca-Si-based bioactive dressing includes the following steps:

[0008] (1) Drop tetraethyl orthosilicate into an ethanol / ammonia water mixed solution. After mixing evenly, add an aqueous solution of calcium nitrate tetrahydrate, stir and react, centrifuge, wash, dry to obtain a powder, and then sinter to obtain Ca-Si-based bioactive particles;

[0009] (2) Prepare an aqueous solution of polyvinyl alcohol and an aqueous solution of polyethylene glycol separately. Mix the aqueous solution of polyvinyl alcohol and the aqueous solution of polyethylene glycol evenly, and then add Ca-Si-based bioactive particles. Stir and react at 80-90 °C for 1-4 hours to obtain a Ca-Si-based bioactive dressing.

[0010] Preferably, the ratio of tetraethyl orthosilicate to calcium nitrate tetrahydrate in step (1) is 1-2.5 mL: 0.1-0.5 g; more preferably 1.5-2 mL: 0.2-0.3 g.

[0011] Preferably, the ethanol / ammonia water mixed solution in step (1) is obtained by mixing anhydrous ethanol, water and ammonia water in a volume ratio of (75-90):(5-20):(0.5-5) and stirring at 35-45 °C for 10-30 minutes; more preferably, the volume ratio of anhydrous ethanol, water and ammonia water is (85-90):(9.2-13):(0.8-2).

[0012] Preferably, the volume ratio of tetraethyl orthosilicate to the ethanol / ammonia water mixed solution in step (1) is 1-2.5:500.

[0013] Preferably, the "mixing evenly" in step (1) means continuous stirring at 35-45 °C for 5-10 minutes.

[0014] Preferably, the aqueous solution of calcium nitrate tetrahydrate in step (1) is obtained by adding 0.1-0.5 g of calcium nitrate tetrahydrate to 100 mL of water and stirring for 20-50 minutes, that is, the concentration of the aqueous solution of calcium nitrate tetrahydrate is 1-5 mg / mL; more preferably 2-3 mg / mL.

[0015] Preferably, the temperature of the stirring reaction in step (1) is 35-45 °C; the time is 5-10 hours; more preferably, the reaction is carried out at 40 °C for 6-8 hours.

[0016] Preferably, the rotation speed of the centrifugation in step (1) is 3000-5000 revolutions per minute, and the centrifugation time is 20-50 minutes.

[0017] Preferably, the "washing" in step (1) means washing 2-5 times with ethanol and water.

[0018] Preferably, the "drying" in step (1) means drying at 45-65 °C for 8-12 hours.

[0019] Preferably, the temperature of the sintering in step (1) is 550-650 °C, and the heat preservation time is 2-4 hours; more preferably, it is 600-620 °C for heat preservation for 2-3 hours.

[0020] Preferably, the concentration of the aqueous solution of polyvinyl alcohol in step (2) is 10-100 g / L; more preferably 50-60 g / L.

[0021] Preferably, the concentration of the polyethylene glycol aqueous solution in step (2) is 5-50 g / L; more preferably 10-20 g / L.

[0022] Preferably, the volume ratio of the polyvinyl alcohol aqueous solution to the polyethylene glycol aqueous solution in step (2) is 1:1.

[0023] Preferably, in step (2), the molecular weight of polyvinyl alcohol is 9000-124000 Da, more preferably 85000-31000 Da; the molecular weight of polyethylene glycol is 200-6000 Da; more preferably 800-4000 Da.

[0024] Preferably, the mass ratio of the Ca-Si-based bioactive particles in step (2) to the total volume of the solution after mixing the polyvinyl alcohol aqueous solution and the polyethylene glycol aqueous solution is 20-200 g / L; more preferably 50-80 g / L.

[0025] The present invention also provides a Ca-Si-based bioactive dressing prepared by the above preparation method.

[0026] The present invention also provides the application of the above Ca-Si-based bioactive dressing in the preparation of products for skin wound repair.

[0027] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0028] 1. Polyethylene glycol and polyvinyl alcohol themselves have good biocompatibility and high safety for biomedical materials; moreover, the two increase the viscosity of the dressing, prolong the action time of the dressing at the damaged site, and enhance the therapeutic effect of the active ingredients.

[0029] 2. The introduction of Ca-Si-based bioactive particles can promote cell proliferation and differentiation, thereby accelerating the rapid repair of the damaged site.

[0030] 3. The dressing components of the present invention are uniform and present a colorless and transparent state, which is convenient for observing the repair status of the damaged site in real time during use. Description of the Drawings

[0031] Figure 1 It is a bar chart showing the promotion of endothelial cell proliferation by the Ca-Si-based bioactive dressing prepared in Example 1 of the present invention and Comparative Example 1 (control group).

[0032] Figure 2 It is a fluorescence image showing the promotion of endothelial cell spreading by the Ca-Si-based bioactive dressing prepared in Example 1 of the present invention (right figure) and Comparative Example 1 (control group, left figure).

[0033] Figure 3Picture of the state of solution C in Example 1 of the present invention (left) and the state of solution C in Example 2 (right).

[0034] Figure 4 Physical picture of the Ca-Si based bioactive dressing obtained in Comparative Example 2 of the present invention.

[0035] Figure 5 Physical picture of the Ca-Si based bioactive dressing obtained in Comparative Example 3 of the present invention. Detailed implementation manners

[0036] The present invention will be further described in detail below in conjunction with examples and drawings, but the implementation manners of the present invention are not limited thereto.

[0037] For those not specified in the examples of the present invention, they are carried out according to conventional conditions or conditions recommended by the manufacturer. Raw materials, reagents, etc. without indicating the manufacturer can be obtained as conventional products through commercial purchase.

[0038] The present invention will be further described below in conjunction with specific examples. Unless otherwise specified, the following solution concentrations (%) are all the ratio of solute mass (g) to solvent volume (mL).

[0039] Example 1

[0040] This example provides a preparation method of a Ca-Si based bioactive dressing, including the following steps:

[0041] The first part: Preparation of Ca-Si based bioactive particles

[0042] 1) Mix absolute ethanol, water and ammonia water according to a volume ratio of 85:13:2, stir at 40 °C to obtain 500 mL of solution A, the stirring time is 20 minutes, and the concentration of ammonia water is 25-28%.

[0043] 2) Weigh 0.2 g of calcium nitrate tetrahydrate and add it to 100 mL of water to obtain solution B, and the stirring time is 30 minutes.

[0044] 3) Slowly add 1.5 mL of tetraethyl orthosilicate to solution A, and continuously stir at 40 °C for 5 minutes during the addition process to obtain solution C.

[0045] 4) Add solution B to solution C, and continuously stir at 40 °C to obtain a milky white solution, and the stirring time is 6 hours.

[0046] 5) Centrifuge the milky white solution in 5) to obtain a white material, the centrifugation rate is 4000 revolutions per minute, and the centrifugation time is 30 minutes.

[0047] 6) First, wash the white material obtained in 6) with absolute ethanol for the first time, then wash it with water for the second time, and finally wash it with absolute ethanol for the third time.

[0048] 7) Dry the final material obtained in 7) at 50 °C for 10 hours.

[0049] 8) Place the obtained powder in a box furnace for sintering. The sintering temperature is 600 °C and the holding time is 2 hours to obtain Ca-Si-based bioactive particles.

[0050] Second part: Preparation of Ca-Si-based bioactive dressing

[0051] 1) Add polyvinyl alcohol with a molecular weight of 85000 Da to 100 mL of aqueous solution according to a mass-volume ratio of 5%, and stir at 90 °C for 40 minutes to obtain solution A1.

[0052] 2) Add polyethylene glycol with a molecular weight of 800 Da to 100 mL of aqueous solution according to a mass-volume ratio of 1%, and stir at 30 °C for 40 minutes to obtain solution B1.

[0053] 3) Mix solution A1 and solution B1 in a volume ratio of 1:1 to obtain solution C, and stir for 40 minutes.

[0054] 4) Weigh Ca-Si-based bioactive particles according to a mass-volume ratio of 5% and add them to solution C. Stir at 90 °C for 2 hours for uniform reaction to obtain Ca-Si-based bioactive dressing after sufficient reaction.

[0055] Test experimental process for promoting endothelial cell proliferation: Extract the bioactive dressing with serum-free medium at a concentration of 0.1 g / mL, and place it in a constant temperature shaker at 37 °C for shaking extraction to obtain the extract. Then use the CCK-8 kit to detect the viability of cells. The cell seeding density is 10 4 cells / mL (500 μL / well). Co-culture the extract with cells for 1, 3, and 5 days. After the culture time ends, aspirate the old medium, add the CCK-8 working solution, incubate at 37 °C for 2 hours, and transfer the reaction solution to a new 96-well plate after the reaction. Use a multifunctional microplate reader to detect the absorbance value of the reaction solution at a wavelength of 450 nm.

[0056] Test experimental procedure for promoting endothelial cell spreading: Observe cell spreading through cytoskeleton staining. After culturing the cells with the above-mentioned extract for 48 hours, discard the old culture medium, wash the cells three times with PBS, and then fix the cells with 4% paraformaldehyde solution for 10 minutes. In the second step, wash the cells with PBS containing 0.1% Triton X-100 for 5 minutes. In the third step, dilute the green fluorescent probe staining solution with PBS containing 0.1% Triton at a ratio of 1:100 to obtain the cytoskeleton staining working solution. Add the staining working solution prepared in the third step to the sample wells after the second-step treatment, incubate at room temperature for 30 minutes, and then wash the wells three times with PBS containing 0.1% Triton. Use DAPI solution (1 μg / ml) to counterstain the cell nuclei for 5 minutes, then discard the DAPI solution and wash the wells three times with PBS. Observe the surface of the stained samples using a laser confocal microscope.

[0057] Figure 1 Bar graph showing the promotion of endothelial cell proliferation by the Ca-Si-based bioactive dressing prepared in Example 1 of the present invention and Comparative Example 1 (control group). Figure 1 It can be seen that the Ca-Si-based bioactive dressing prepared in Example 1 of the present invention can significantly improve the cell survival rate of the dressing.

[0058] Figure 2 Fluorescence image showing the promotion of endothelial cell spreading by the Ca-Si-based bioactive dressing prepared in Example 1 of the present invention and Comparative Example 1 (control group). Figure 2 It can be seen that the Ca-Si-based bioactive dressing prepared in Example 1 of the present invention is more conducive to promoting endothelial cell spreading and achieving wound healing.

[0059] Comparative Example 1

[0060] Preparation of a dressing without Ca-Si-based bioactive particles

[0061] 1) Add polyvinyl alcohol with a molecular weight of 85000 Da to 100 mL of aqueous solution at a mass-volume ratio of 5%, and stir at 90 °C for 40 minutes to obtain Solution A1.

[0062] 2) Add polyethylene glycol with a molecular weight of 800 Da to 100 mL of aqueous solution at a mass-volume ratio of 1%, and stir at 30 °C for 40 minutes to obtain Solution B1.

[0063] 3) Mix Solution A1 and Solution B1 in a volume ratio of 1:1 to obtain Solution C, and stir for 40 minutes to obtain a dressing without Ca-Si-based bioactive particles.

[0064] Comparative Example 2

[0065] This comparative example provides a method for preparing a Ca-Si-based bioactive dressing, which includes the following steps:

[0066] Part 1: Preparation of Ca-Si-based bioactive particles

[0067] 1) Mix anhydrous ethanol, water, and ammonia water in a volume ratio of 85:13:2, stir at 40 °C to obtain 500 mL of solution A, with a stirring time of 20 minutes and the concentration of ammonia water being 25-28%.

[0068] 2) Weigh 0.2 g of calcium nitrate tetrahydrate and add it to 100 mL of water to obtain solution B, with a stirring time of 30 minutes.

[0069] 3) Slowly add 1.5 mL of tetraethyl orthosilicate to solution A, and continuously stir at 40 °C for 5 minutes during the addition process to obtain solution C.

[0070] 4) Add solution B to solution C and continuously stir at 40 °C to obtain a milky white solution, with a stirring time of 6 hours.

[0071] 5) Centrifuge the milky white solution in step 5) to obtain a white material, with a centrifuge speed of 4000 revolutions per minute and a centrifuge time of 30 minutes.

[0072] 6) First, wash the white material obtained in step 6) with anhydrous ethanol for the first time, then wash it with water for the second time, and finally wash it with anhydrous ethanol for the third time.

[0073] 7) Dry the final material obtained in step 7) at 50 °C for 10 hours.

[0074] 8) Place the obtained powder in a box furnace for sintering, with a sintering temperature of 600 °C and a holding time of 2 hours to obtain Ca-Si-based bioactive particles.

[0075] Part 2: Preparation of Ca-Si-based bioactive dressing

[0076] 1) Add polyvinyl alcohol with a molecular weight of 85000 Da to 100 mL of aqueous solution according to a mass-volume ratio of 11%, stir at 90 °C for 40 minutes to obtain solution A1.

[0077] 2) Add polyethylene glycol with a molecular weight of 800 Da to 100 mL of aqueous solution according to a mass-volume ratio of 0.3%, stir at 30 °C for 40 minutes to obtain solution B1.

[0078] 3) Mix solution A1 and solution B1 in a volume ratio of 1:1 to obtain solution C, with a stirring time of 40 minutes.

[0079] 4) Weigh the Ca-Si based bioactive particles according to a mass-volume ratio of 5%, add them to solution C, and stir at 90 °C for 2 hours for uniform reaction. After sufficient reaction, a Ca-Si based bioactive dressing is obtained.

[0080] The Ca-Si based bioactive dressing obtained in Comparative Example 2 is as Figure 4 shown. It can be seen that its viscosity has decreased relatively, it does not have the adhesiveness of a dressing, and it cannot achieve effective application of medicine.

[0081] Comparative Example 3

[0082] This comparative example provides a preparation method of a Ca-Si based bioactive dressing, including the following steps:

[0083] The first part: Preparation of Ca-Si based bioactive particles

[0084] 1) Mix absolute ethanol, water and ammonia water according to a volume ratio of 85:13:2, stir at 40 °C to obtain 500 mL of solution A, with a stirring time of 20 minutes and the concentration of ammonia water being 25-28%.

[0085] 2) Weigh 0.2 g of calcium nitrate tetrahydrate and add it to 100 mL of water to obtain solution B, with a stirring time of 30 minutes.

[0086] 3) Slowly add 1.5 mL of tetraethyl orthosilicate to solution A, and continuously stir at 40 °C for 5 minutes during the addition process to obtain solution C.

[0087] 4) Add solution B to solution C and continuously stir at 40 °C to obtain a milky white solution, with a stirring time of 6 hours.

[0088] 5) Centrifuge the milky white solution in step 5) to obtain a white material, with a centrifugation rate of 4000 revolutions per minute and a centrifugation time of 30 minutes.

[0089] 6) First wash the white material obtained in step 6) with absolute ethanol for the first time, then wash it with water for the second time, and finally wash it with absolute ethanol for the third time.

[0090] 7) Dry the final material obtained in step 7) at 50 °C for 10 hours.

[0091] 8) Place the obtained powder in a box furnace for sintering, with a sintering temperature of 600 °C and a heat preservation time of 2 hours to obtain Ca-Si based bioactive particles.

[0092] The second part: Preparation of Ca-Si based bioactive dressing

[0093] 1) Add polyvinyl alcohol with a molecular weight of 85000 Da to 100 mL of aqueous solution at a mass-volume ratio of 0.1%, and stir at 90 °C for 40 minutes to obtain solution A1.

[0094] 2) Add polyethylene glycol with a molecular weight of 800 Da to 100 mL of aqueous solution at a mass-volume ratio of 8%, and stir at 30 °C for 40 minutes to obtain solution B1.

[0095] 3) Mix solution A1 and solution B1 in a volume ratio of 1:1 to obtain solution C, and stir for 40 minutes.

[0096] 4) Weigh Ca-Si-based bioactive particles at a mass-volume ratio of 5% and add them to solution C, and stir at 90 °C for 2 hours for uniform reaction to obtain a Ca-Si-based bioactive dressing after sufficient reaction.

[0097] The Ca-Si-based bioactive dressing obtained in Comparative Example 3 is as Figure 5 shown. It can be seen that its viscosity has decreased relatively, and it does not have the adhesiveness of a dressing, so it cannot achieve effective application of applying medicine.

[0098] Example 2

[0099] This example provides a preparation method of a Ca-Si-based bioactive dressing, including the following steps:

[0100] The first part: Preparation of Ca-Si-based bioactive particles

[0101] 1) Mix absolute ethanol, water and ammonia water in a volume ratio of 90:9.2:0.8, and stir at 40 °C to obtain 500 mL of solution A. The stirring time is 20 minutes, and the concentration of ammonia water is 25-28%.

[0102] 2) Weigh 0.3 g of calcium nitrate tetrahydrate and add it to 100 mL of water to obtain solution B. The stirring time is 30 minutes.

[0103] 3) Slowly add 2 mL of tetraethyl orthosilicate to solution A, and continuously stir at 40 °C for 5 minutes during the addition process to obtain solution C.

[0104] 4) Add solution B to solution C, and continuously stir at 40 °C to obtain a milky white solution. The stirring time is 8 hours.

[0105] 5) Centrifuge the milky white solution in 5) to obtain a white material. The centrifugation rate is 4500 revolutions per minute, and the centrifugation time is 30 minutes.

[0106] 6) First wash the white material obtained in 6) with absolute ethanol for the first time, then wash it with water for the second time, and finally wash it with absolute ethanol for the third time.

[0107] 7) Dry the final material obtained in 7) at 60 °C for 10 hours.

[0108] 8) Place the obtained powder in a box furnace for sintering. The sintering temperature is 620 °C and the holding time is 3 hours to obtain Ca-Si-based bioactive particles.

[0109] Second part: Preparation of Ca-Si-based bioactive dressing

[0110] 1) Add polyvinyl alcohol with a molecular weight of 31000 Da to 100 mL of aqueous solution according to a mass-volume ratio of 6%, and stir at 90 °C for 60 minutes to obtain solution A1.

[0111] 2) Add polyethylene glycol with a molecular weight of 4000 Da to 100 mL of aqueous solution according to a mass-volume ratio of 2%, and stir at 30 °C for 60 minutes to obtain solution B1.

[0112] 3) Mix solution A1 and solution B1 in a volume ratio of 1:1 to obtain solution C, and the stirring time is 60 minutes.

[0113] 4) Weigh Ca-Si-based bioactive particles according to a mass-volume ratio of 8% and add them to solution C, stir at 90 °C for 2 hours for uniform reaction, and fully react to obtain Ca-Si-based bioactive dressing.

[0114] Figure 3 This is a picture of the state of solution C in Example 1 (left) and the state of solution C in Example 2 (right) of the present invention. The right picture shows that it adheres more to the glass wall, indicating that the viscosity of the dressing can be regulated. When its concentration is not appropriate, it will appear in a liquid state, such as Figure 4 and 5 shown in Comparative Examples 2 and 3.

[0115] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principle of the present invention shall be equivalent replacement methods and are all included in the protection scope of the present invention.

Claims

1. A method for preparing a Ca-Si based bioactive dressing, characterized in that: The following steps are involved: (1) adding tetraethyl orthosilicate dropwise to an ethanol / ammonia mixed solution, mixing evenly, then adding a tetrahydrate calcium nitrate aqueous solution, stirring for reaction, centrifuging, washing, and drying to obtain a powder, and then sintering to obtain Ca-Si based bioactive particles; (2) preparing a polyvinyl alcohol aqueous solution and a polyethylene glycol aqueous solution respectively, mixing the polyvinyl alcohol aqueous solution and the polyethylene glycol aqueous solution evenly, adding Ca-Si based bioactive particles, stirring and reacting at 80-90° C. for 1-4 hours, and obtaining a Ca-Si based bioactive dressing.

2. The method for preparing a Ca-Si based bioactive dressing according to claim 1, characterized in that: The ratio of tetraethyl orthosilicate to calcium nitrate tetrahydrate in step (1) is 1-2.5 mL: 0.1-0.5 g; And / or, the stirring reaction in step (1) is carried out at a temperature of 35 to 45° C. and for 5 to 10 hours; And / or, the sintering temperature in step (1) is 550-650° C., and the holding time is 2-4 hours.

3. The method for preparing a Ca-Si based bioactive dressing according to claim 2, characterized in that: The ratio of ethyl orthosilicate to calcium nitrate tetrahydrate in step (1) is 1.5-2 mL: 0.2-0.3 g; And / or, the stirring reaction in step (1) is carried out at a temperature of 35 to 45° C. and for 6 to 8 hours; And / or, the sintering temperature in step (1) is 600-620° C., and the holding time is 2-3 hours.

4. A method for preparing a Ca-Si based bioactive dressing according to claim 1 or 2, characterized in that: The concentration of the polyvinyl alcohol aqueous solution in step (2) is 10 to 100 g / L; And / or, the concentration of the polyethylene glycol aqueous solution in step (2) is 5 to 50 g / L; And / or, the volume ratio of the polyvinyl alcohol aqueous solution to the polyethylene glycol aqueous solution in step (2) is 1:1; And / or, in step (2), the molecular weight of polyvinyl alcohol is 9000-124000 Da; the molecular weight of polyethylene glycol is 200-6000 Da.

5. The method for preparing a Ca-Si based bioactive dressing according to claim 4, characterized in that: The concentration of the polyvinyl alcohol aqueous solution in step (2) is 50-60 g / L; And / or, the concentration of the polyethylene glycol aqueous solution in step (2) is 10 to 20 g / L; And / or, the volume ratio of the polyvinyl alcohol aqueous solution to the polyethylene glycol aqueous solution in step (2) is 1:1; And / or, in step (2), the molecular weight of polyvinyl alcohol is 85000-31000 Da; the molecular weight of polyethylene glycol is 800-4000 Da.

6. A method for preparing a Ca-Si based bioactive dressing according to claim 1, 2 or 4, characterized in that: The mass ratio of the Ca-Si based bioactive particles in step (2) to the total volume of the solution after the polyvinyl alcohol aqueous solution and the polyethylene glycol aqueous solution are mixed is 20 to 200 g / L.

7. The method for preparing a Ca-Si based bioactive dressing according to claim 6, characterized in that: The mass ratio of the Ca-Si based bioactive particles in step (2) to the total volume of the solution after the polyvinyl alcohol aqueous solution and the polyethylene glycol aqueous solution are mixed is 50 to 80 g / L.

8. A method for preparing a Ca-Si based bioactive dressing according to claim 1, 2 or 4, characterized in that: The ethanol / ammonia aqueous solution of step (1) is prepared by mixing anhydrous ethanol, water and ammonia aqueous solution in a volume ratio of (75-90): (5-20): (0.5-5) at 35-45° C. and stirring for 10-30 minutes; And / or, the volume ratio of the tetraethyl orthosilicate and the ethanol / ammonia aqueous solution in step (1) is 1-2.5:500; And / or, the concentration of the calcium nitrate tetrahydrate aqueous solution in step (1) is 1 to 5 mg / mL.

9. A Ca-Si based bioactive dressing prepared by the preparation method according to any one of claims 1 to 8.

10. Use of the Ca-Si based bioactive dressing according to claim 9 in preparing products for skin wound repair.

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