A skin-revitalizing, moisturizing and soothing composition and preparation method thereof

Abstract

The present invention discloses a skin-revitalizing, nourishing and soothing composition and a preparation method thereof, and belongs to the technical field of cosmetics. The skin-revitalizing, nourishing and soothing composition comprises a plant extract, a moisturizing agent, a chelating agent, a thickener, sodium heparin, an emollient, an antioxidant, an emulsifier, a pH regulator, and a preservative. The present invention introduces a plant extract and rationally adjusts the proportions of components such as the plant extract, the moisturizing agent, the chelating agent, the thickener, sodium heparin, an emollient, an antioxidant, an emulsifier, etc., so that the composition is mild and non-irritating, easy to apply and absorb, can enhance skin elasticity, and has excellent soothing, anti-stress, repairing, and anti-aging effects; the preparation method provided by the present invention is simple and easy to operate, and the quality of the prepared composition is stable.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a skin-activating, moisturizing and soothing composition and a preparation method thereof. Background Art

[0002] As people's focus on health and quality of life increases, consumer demand for cosmetics will continue to grow. While existing cosmetics on the market offer a wide variety of products with diverse functions, they still suffer from some common drawbacks and limitations. For example, most cream-based cosmetics are overly greasy, leaving the skin feeling sticky and poorly absorbed. They also lack effective anti-aging effects and can easily cause skin irritation, triggering allergic reactions such as redness, swelling, and itching. Summary of the Invention

[0003] The object of the present invention is to overcome the deficiencies of the above-mentioned prior art and to provide a skin-revitalizing, nourishing and soothing composition and a preparation method thereof.

[0004] To achieve the above object, the technical solution adopted by the present invention is:

[0005] In a first aspect, the present invention provides a skin-revitalizing, moisturizing and soothing composition, comprising the following components in percentage by mass: 0.5-5% plant extract, 5-6.9% moisturizer, 0.01-0.05% chelating agent, 0.05-0.25% thickener, 0.01-0.1% heparin sodium, 16-28% emollient, 0.1-0.5% antioxidant, 4.5-7% emulsifier, 1.1-1.3% pH adjuster, 0.1-0.5% preservative, and the balance water, wherein the plant extract comprises at least one of agave stem extract, kava root extract, Centella asiatica extract, gentian root extract, and white violet extract.

[0006] As a preferred embodiment of the present invention, the plant extracts include agave stem extract, kava root extract, Centella asiatica extract, gentian root extract, and white osmanthus extract, and the mass ratio of the kava root extract, Centella asiatica extract, gentian root extract, white osmanthus extract, and agave stem extract is (1~4):(0.5~2):(3~6):(0.5~2):10.

[0007] Agave stem extract is rich in active substances such as polysaccharides, amino acids, polyphenols, sterols, flavonoids, sulfur-containing amino acids, etc. It not only has a moisturizing effect, can promote skin metabolism, repair damaged skin tissue, but also has certain anti-inflammatory, antibacterial and antioxidant effects; Kava pepper root extract contains ingredients such as kavalactone and kavalpyrone, which have anti-inflammatory and soothing effects on the skin; Centella asiatica extract contains active ingredients such as asiaticoside, hydroxymadecasoside, asiatic acid and hydroxymadecassic acid, which can promote collagen synthesis and promote the secretion of mucopolysaccharides, and also has certain antibacterial, anti-inflammatory and antioxidant effects; Gentiana root extract contains active ingredients such as gentiopicroside, gentiobiose and gentisic acid, which can promote the secretion of cholesterol by epidermal cells, improve skin roughness, and have certain anti-inflammatory effects; White iris extract is rich in polyphenols and flavonoids, which has antioxidant effects, can scavenge free radicals in the body, help delay skin aging, repair skin damage, and strengthen the skin barrier.

[0008] The inventors have discovered that when Agave stem extract, Kava root extract, Centella asiatica extract, Gentiana root extract, and White Rose extract are combined in specific ratios, they exhibit a significant synergistic effect, significantly improving skin firmness and elasticity, while also providing soothing, stress-relieving, repairing, and anti-aging benefits. This may be due to the fact that the extracts in this invention contain different active ingredients, which together promote the expression of anti-aging genes and stimulate collagen production in fibroblasts through different mechanisms. Furthermore, the inventors unexpectedly discovered that when Agave stem extract, Kava root extract, Centella asiatica extract, Gentiana root extract, and White Rose extract are combined in specific ratios, they can reduce the irritation of compositions containing high emulsifier content.

[0009] Furthermore, the mass ratio of the Piper methysticum root extract, Centella asiatica extract, Gentiana scabra root extract, Scutellaria baicalensis extract, and Agave equisetum stem extract is (2-3):(1-1.5):(4-5):(1-1.5):10.

[0010] Furthermore, the agave stem extract is prepared by the following method: the agave stem is crushed, dried, and sieved, and then added to an 80% ethanol solution at a solid-liquid ratio of 1g:20mL for ultrasonic extraction, and the filter residue and filtrate are separated by filtration; the filter residue is added to an 80% ethanol solution at a solid-liquid ratio of 1g:15mL for ultrasonic extraction, and the filter residue and filtrate are separated by filtration, the filtrate is combined, concentrated, dried, and then sterilized to obtain the agave stem extract.

[0011] Furthermore, the kava root extract is prepared by the following method: the kava root is crushed, dried, and sieved, and then added to an 80% ethanol solution at a solid-liquid ratio of 1g:20mL for ultrasonic extraction, and the filter residue and filtrate are separated by filtration; the filter residue is added to an 80% ethanol solution at a solid-liquid ratio of 1g:15mL for ultrasonic extraction, and the filter residue and filtrate are separated by filtration, the filtrate is combined, concentrated, dried, and then sterilized to obtain the kava root extract.

[0012] Furthermore, the Centella asiatica extract is prepared by the following method: the whole plant of Centella asiatica is crushed, dried, and sieved, and then added to an 80% ethanol solution at a solid-liquid ratio of 1g:20mL for ultrasonic extraction, and the filter residue and filtrate are separated by filtration; the filter residue is added to an 80% ethanol solution at a solid-liquid ratio of 1g:10mL for ultrasonic extraction, and the filter residue and filtrate are separated by filtration, the filtrate is combined, concentrated, dried, and then sterilized to obtain the Centella asiatica extract.

[0013] Furthermore, the gentian root extract is prepared by the following method: the gentian root is crushed, dried, and sieved, and then added to an 80% ethanol solution at a solid-liquid ratio of 1g:20mL for ultrasonic extraction, and the filter residue and filtrate are separated by filtration; the filter residue is added to an 80% ethanol solution at a solid-liquid ratio of 1g:15mL for ultrasonic extraction, and the filter residue and filtrate are separated by filtration, the filtrate is combined, concentrated, dried, and then sterilized to obtain the gentian root extract.

[0014] Furthermore, the Bletilla striata extract is prepared by the following method: the Bletilla striata root is crushed, dried, and sieved, and then added to an 80% ethanol solution at a solid-liquid ratio of 1g:20mL for ultrasonic extraction, and the residue and filtrate are separated by filtration; the residue is added to an 80% ethanol solution at a solid-liquid ratio of 1g:10mL for ultrasonic extraction, and the residue and filtrate are separated by filtration, the filtrate is combined, concentrated, dried, and then sterilized to obtain the Bletilla striata extract.

[0015] As a preferred embodiment of the present invention, the skin-revitalizing, moisturizing and soothing composition includes the following components in percentage by mass: 2-4% plant extract, 6-6.5% moisturizer, 0.02-0.04% chelating agent, 0.16-0.2% thickener, 0.02-0.05% heparin sodium, 20.4-21.8% emollient, 0.12-0.3% antioxidant, 5.1-6.3% emulsifier, 1.1-1.3% pH adjuster, 0.1-0.5% preservative, and the balance water.

[0016] As a preferred embodiment of the present invention, the moisturizing agent includes at least one of sodium hyaluronate, betaine, glycerol, glyceryl polyether-26, allantoin, panthenol, trehalose, dipropylene glycol, and butylene glycol.

[0017] As a preferred embodiment of the present invention, the thickener includes carbomer.

[0018] As a preferred embodiment of the present invention, the emollient includes at least one of sea buckthorn oil, jojoba oil, cyclopentasiloxane, cyclohexasiloxane, caprylic / capric triglyceride, shea butter, isopropyl myristate, ethylhexyl palmitate, hydrogenated polyisobutene, macadamia seed oil, hydrogenated polydecene, isodecyl neopentanoate, and dicaprylyl carbonate.

[0019] Further preferably, in the skin-revitalizing, nourishing and soothing composition, the mass percentages of the various components of the emollient are as follows: sea buckthorn oil 0.05-0.3%, jojoba oil 0.5-3%, cyclopentasiloxane 0.4-2.4%, cyclohexasiloxane 0.1-0.6%, caprylic / capric triglyceride 0.5-2%, avocado butter 0.1-0.5%, isopropyl myristate 3-10%, ethylhexyl palmitate 3-10%, hydrogenated polyisobutene 1-4%, and macadamia seed oil 0.05-0.3%.

[0020] As a preferred embodiment of the present invention, the emulsifier includes at least one of cetearyl alcohol, cetearyl glucoside, glyceryl stearate, and PEG-100 stearate.

[0021] Further preferably, in the skin-rejuvenating, moisturizing and soothing composition, the mass percentages of the various components of the emulsifier are as follows: 1-3% cetearyl alcohol, 1-4% first mixed emulsifier, and 0.5-2% second mixed emulsifier, wherein the first mixed emulsifier is compounded with cetearyl alcohol-80 and cetearyl glucoside-20 in a mass ratio of (75-85):(15-25), and the second mixed emulsifier is compounded with glyceryl stearate-65 and PEG-100 stearate-35 in a mass ratio of (50-75):(25-50).

[0022] Furthermore, in the skin-revitalizing, moisturizing and soothing composition, the mass percentages of the various components of the emollient are as follows: sea buckthorn oil 0.05~0.3%, jojoba oil 0.5~3%, cyclopentasiloxane 0.4~2.4%, cyclohexasiloxane 0.1~0.6%, caprylic / capric triglyceride 0.5~2%, avocado butter 0.1~0.5%, isopropyl myristate 3~10%, ethylhexyl palmitate 3~10%, hydrogenated polyisobutene 1~4%, and macadamia seed oil 0.05~0.3%; in the skin-revitalizing, moisturizing and soothing composition, the mass percentages of the various components of the emulsifier are as follows: cetearyl alcohol 1.6~2.5%, a first mixed emulsifier 2~3%, and a second mixed emulsifier 0.8~1.5%. The inventors have found through research that when the components of the emulsifier and the moisturizer are within the above-mentioned range, the aqueous phase component and the oil component in the composition can be fully mixed to form a stable milky white paste, thereby making the texture of the composition smoother and more delicate, improving the spreadability and absorbability of the composition, and being able to gently nourish the skin.

[0023] As a preferred embodiment of the present invention, the pH regulator is a compound of arginine and water, wherein the mass percentage of arginine in the composition is 0.1-0.3%.

[0024] For example, the mass percentage of arginine in the composition can be 0.1%, 0.15%, 0.16%, 0.2%, 0.25%, 0.3% or a range consisting of any two groups of values therein.

[0025] As a preferred embodiment of the present invention, the antioxidant includes tocopherol acetate.

[0026] As a preferred embodiment of the present invention, the skin-revitalizing, nourishing and soothing composition further comprises the following components in percentage by mass: 0.01-0.5% of essence.

[0027] The essence can be at least one of lavender oil, rose flower oil, rose flower oil, camellia oil, and daily essence.

[0028] In a second aspect, the present invention provides a method for preparing the skin-revitalizing, moisturizing and soothing composition as described in the first aspect, comprising the following steps:

[0029] S1. Mix water, humectant, chelating agent, thickener, and heparin sodium, heat to 82-85°C, and stir until completely dissolved to obtain phase P1.

[0030] S2, mixing the emulsifier, emollient and antioxidant and heating them to 78-82°C to dissolve them completely to obtain phase P2;

[0031] S3. Add phase P2 to phase P1 under stirring, homogenize for 5-8 minutes until completely dissolved, keep warm for 15-20 minutes, and defoam.

[0032] S4, cooling the mixture after defoaming in step S3 to 64-66°C, adding pH regulators in sequence, stirring evenly, and then continuing to cool to a temperature not higher than 45°C, adding plant extracts and preservatives in sequence, and stirring evenly;

[0033] S5. Adjust the temperature of the mixture obtained in step S4 to 38-40° C., filter through a 100-mesh filter, discharge the mixture, and allow it to stand to obtain the skin-revitalizing, moisturizing and soothing composition.

[0034] Compared with the prior art, the present invention has the following beneficial effects:

[0035] The present invention introduces plant extracts and rationally adjusts the proportions of components such as the plant extracts, moisturizers, chelating agents, thickeners, heparin sodium, emollients, antioxidants, and emulsifiers to make the composition mild and non-irritating, easy to apply and absorb, capable of improving skin elasticity, and having excellent soothing, anti-stress, repairing, and anti-aging effects. The preparation method provided by the present invention is simple and easy to perform, convenient to operate, and the quality of the prepared composition is stable. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 This is a photo of the composition obtained in Example 1 provided by the present invention;

[0037] Figure 2 The test results provided for the effectiveness test 1 of the present invention;

[0038] Figure 3 The test results of Example 2 of the present invention are provided. DETAILED DESCRIPTION

[0039] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below with reference to specific embodiments and comparative examples. The purpose is to provide a detailed understanding of the content of the present invention, but not to limit the present invention. All other embodiments obtained by those of ordinary skill in the art without creative work are within the scope of protection of the present invention.

[0040] Unless otherwise specified, the components and raw materials used in the examples and comparative examples of the present invention are all commercially available raw materials, and the components and raw materials used in each parallel experiment are all of the same kind.

[0041] The agave stem extracts in the following examples and comparative examples were prepared by the following method:

[0042] The agave stem is crushed, dried, and sieved, and then added to an 80% ethanol solution at a solid-liquid ratio of 1 g:20 mL, ultrasonically extracted at a power of 300 W for 45 minutes, and filtered to separate the filter residue and the filtrate; the filter residue is added to an 80% ethanol solution at a solid-liquid ratio of 1 g:15 mL, ultrasonically extracted at a power of 300 W for 45 minutes, and filtered to separate the filter residue and the filtrate, the filtrates are combined, concentrated by rotary evaporation to 1 / 10 of the volume of the combined filtrate, dried, and sterilized at 100-120° C. to obtain the agave stem extract.

[0043] The Kava root extracts in the following examples and comparative examples were prepared by the following method:

[0044] The Piper methysticum root is crushed, dried, and sieved, and then added to an 80% ethanol solution at a solid-liquid ratio of 1 g:20 mL, ultrasonically extracted at a power of 300 W for 45 minutes, and filtered to separate the filter residue and the filtrate; the filter residue is added to an 80% ethanol solution at a solid-liquid ratio of 1 g:15 mL, ultrasonically extracted at a power of 300 W for 45 minutes, and filtered to separate the filter residue and the filtrate, the filtrates are combined, concentrated by rotary evaporation to 1 / 10 of the volume of the combined filtrate, dried, and sterilized at 100-120° C. to obtain the Piper methysticum root extract.

[0045] The Centella asiatica extracts in the following examples and comparative examples were prepared by the following method:

[0046] The whole herb of Centella asiatica is crushed, dried, and sieved, and then added to an 80% ethanol solution at a solid-liquid ratio of 1 g:20 mL for ultrasonic extraction, and the filter residue and the filtrate are separated by filtration; the filter residue is added to an 80% ethanol solution at a solid-liquid ratio of 1 g:10 mL for ultrasonic extraction, and the filter residue and the filtrate are separated by filtration, the power of each ultrasonic extraction is 300 W, and the time of each ultrasonic extraction is 45 min. The filtrates are combined, concentrated by rotary evaporation to 1 / 10 of the volume of the combined filtrate, dried, and sterilized at 100-120°C to obtain the Centella asiatica extract.

[0047] The gentian root extracts in the following examples and comparative examples were prepared by the following method:

[0048] The gentian root is crushed, dried, and sieved, and then added to an 80% ethanol solution at a solid-liquid ratio of 1 g:20 mL, ultrasonically extracted at a power of 300 W for 45 minutes, and the residue and filtrate are separated by filtration; the residue is added to an 80% ethanol solution at a solid-liquid ratio of 1 g:15 mL, ultrasonically extracted at a power of 300 W for 45 minutes, and the residue and filtrate are separated by filtration, the filtrates are combined, and concentrated by rotary evaporation to 1 / 10 of the volume of the combined filtrate, dried, and sterilized at 100-120° C. to obtain the gentian root extract.

[0049] The Bletilla striata extracts in the following examples and comparative examples were prepared by the following method:

[0050] The root of the Chinese yew vine is crushed, dried, and sieved, and then added to an 80% ethanol solution at a solid-liquid ratio of 1 g:20 mL for ultrasonic extraction, and the filter residue and the filtrate are separated by filtration; the filter residue is added to an 80% ethanol solution at a solid-liquid ratio of 1 g:10 mL for ultrasonic extraction, and the filter residue and the filtrate are separated by filtration, the power of each ultrasonic extraction is 300 W, and the time of each ultrasonic extraction is 45 minutes. The filtrates are combined, concentrated by rotary evaporation to 1 / 10 of the volume of the combined filtrate, dried, and sterilized at 100-120° C. to obtain the Chinese yew vine extract.

[0051] In the present invention, the 80% ethanol solution refers to an ethanol aqueous solution in which the mass percentage of ethanol is 80%.

[0052] Plant extract S1 is a mixture of kava root extract, Centella asiatica extract, gentian root extract, white osmanthus extract, and agave stem extract. The mass ratio of the kava root extract, Centella asiatica extract, gentian root extract, white osmanthus extract, and agave stem extract is 3:1:4:1.5:10.

[0053] Plant extract S2 is prepared by mixing kava root extract, Centella asiatica extract, gentian root extract, iris extract, and agave stem extract in a mass ratio of kava root extract: Centella asiatica extract: gentian root extract: iris extract: agave stem extract = 2:1.5:5:1:10.

[0054] Plant extract S3 is prepared by mixing kava root extract, Centella asiatica extract, gentian root extract, iris extract, and agave stem extract in a mass ratio of kava root extract: Centella asiatica extract: gentian root extract: iris extract: agave stem extract = 1:0.5:6:2:10.

[0055] Plant extract S4 is prepared by mixing kava root extract, Centella asiatica extract, gentian root extract, iris extract, and agave stem extract in a mass ratio of kava root extract: Centella asiatica extract: gentian root extract: iris extract: agave stem extract = 4:2:3:0.5:10.

[0056] The plant extract D1 is a mixture of Centella asiatica extract, Gentiana root extract, White Rose extract, and Agave stem extract. The mass ratio of the Centella asiatica extract, Gentiana root extract, White Rose extract, and Agave stem extract is Centella asiatica extract: Gentiana root extract: White Rose extract: Agave stem extract = 1:4:1.5:10.

[0057] The plant extract D2 is prepared by mixing gentian root extract, white osmanthus extract and agave stem extract in a mass ratio of gentian root extract: white osmanthus extract: agave stem extract = 4:1.5:10.

[0058] Plant Extract D3 In this comparative example, the plant extract consists of a Bletilla striata extract and an Agave equisetum stem extract in a mass ratio of Bletilla striata extract: Agave equisetum stem extract = 1.5:10.

[0059] The plant extract D4 is prepared by mixing gentian root extract and agave stem extract in a mass ratio of gentian root extract: agave stem extract = 4:10.

[0060] Plant extract D5 is prepared by mixing kava root extract, Centella asiatica extract, gentian root extract, iris extract, and agave stem extract in a mass ratio of kava root extract: Centella asiatica extract: gentian root extract: iris extract: agave stem extract = 0.1:0.1:0.1:0.1:10.

[0061] Plant extract D6 is prepared by mixing kava root extract, Centella asiatica extract, gentian root extract, iris extract, and agave stem extract in a mass ratio of kava root extract: Centella asiatica extract: gentian root extract: iris extract: agave stem extract = 0.5:0.5:0.5:0.5:10.

[0062] Plant extract D7 is prepared by mixing kava root extract, Centella asiatica extract, gentian root extract, iris extract, and agave stem extract in a mass ratio of kava root extract: Centella asiatica extract: gentian root extract: iris extract: agave stem extract = 0.5:0.5:1:0.5:10.

[0063] Examples 1 to 4

[0064] An embodiment of the skin-revitalizing, nourishing and soothing composition of the present invention has a formula as shown in Table 1 below.

[0065] The preparation method of the skin-revitalizing, nourishing and soothing composition is as follows:

[0066] S1. Add the components of phase P1 into the main machine, heat to 82-85°C, stir and dissolve completely to obtain phase P1;

[0067] S2. Add the components of phase P2 to the oil phase pot and heat to 78-82°C to dissolve completely to obtain phase P2;

[0068] S3. Evacuate the main unit and pump the P2 phase into the P1 phase under stirring. Homogenize for 5-8 minutes and stir to dissolve completely. Keep warm for 15-20 minutes and defoam.

[0069] S4, cooling the mixture after defoaming in step S3 to 64-66°C, adding the components of phase P3 in sequence, stirring evenly, and then continuing to cool to a temperature not higher than 45°C, adding phase P4, phase P5 and phase P6 in sequence, and stirring evenly;

[0070] S5. Adjust the temperature of the mixture obtained in step S4 to 38-40° C., filter through a 100-mesh filter, discharge the mixture, and allow it to stand to obtain the composition.

[0071] Table 1

[0072]

[0073] Example 5

[0074] The difference between this embodiment and embodiment 1 is that the plant extract used in this embodiment is plant extract S2.

[0075] Example 6

[0076] The difference between this embodiment and embodiment 1 is that the plant extract used in this embodiment is plant extract S3.

[0077] Example 7

[0078] The difference between this embodiment and embodiment 1 is that the plant extract used in this embodiment is plant extract S4.

[0079] Comparative Example 1

[0080] The difference between this comparative example and Example 1 is that the plant extract used in this comparative example is plant extract D1.

[0081] Comparative Example 2

[0082] The difference between this comparative example and Example 1 is that the plant extract used in this comparative example is plant extract D2.

[0083] Comparative Example 3

[0084] The difference between this comparative example and Example 1 is that the plant extract used in this comparative example is plant extract D3.

[0085] Comparative Example 4

[0086] The difference between this comparative example and Example 1 is that the plant extract used in this comparative example is plant extract D4.

[0087] Comparative Example 5

[0088] The difference between this comparative example and Example 1 is that the plant extract used in this comparative example is plant extract D5.

[0089] Comparative Example 6

[0090] The difference between this comparative example and Example 1 is that the plant extract used in this comparative example is plant extract D6.

[0091] Comparative Example 7

[0092] The difference between this comparative example and Example 1 is that the plant extract used in this comparative example is plant extract D7.

[0093] Comparative Example 8

[0094] The difference between this comparative example and Example 1 is that in this comparative example, the mass percentage of cetearyl alcohol in the composition is 6%, the mass percentage of caprylic / capric triglyceride in the composition is 5%, and the mass percentage of water in the P1 phase in the composition is 53.665%.

[0095] The compositions obtained in the above examples and comparative examples were respectively placed in transparent containers. It was found that each composition was a stable milky white paste. For example, the appearance of the composition provided in Example 1 was as follows: Figure 1 shown.

[0096] Effect Test Example 1

[0097] The following tests were conducted using plant extract S1 (corresponding to group S1), plant extract S2 (corresponding to group S2), plant extract S3 (corresponding to group S3), plant extract S4 (corresponding to group S3), plant extract D1 (corresponding to group D1), plant extract D2 (corresponding to group D2), plant extract D3 (corresponding to group D3), plant extract D4 (corresponding to group D4), plant extract D5 (corresponding to group D5), plant extract D6 (corresponding to group D6), and plant extract D7 (corresponding to group D7) as samples:

[0098] Human dermal fibroblasts (HDF) were subcultured and cultured at a rate of 12,000 cells / cm when the cell density reached 80-90%. 2Cells were seeded at a density of 100 μg / mL in 6-well plates in DMEM supplemented with 10% FBS and 1% double-antibody. After overnight attachment, the culture medium was discarded, and equal volumes of PBS buffer (as a control) and each sample group were added to the corresponding wells and cultured for another 48 h. After 48 h of culture, the culture medium was discarded, and the cells were washed with phosphate-buffered saline (DPBS). After trypsinization, the cells were washed with phosphate-buffered saline (DPBS). 500 μL of lysis buffer for RNA extraction was added to each well, and the cells were resuspended by pipetting until no cell pellet was visible and the suspension was essentially clear (indicating sufficient cell lysis). The cell suspension was then temporarily stored at -80°C. RNA was extracted from each group according to the protocol of a commercially available RNA extraction kit. The extracted RNA was dissolved in enzyme-free water, and its concentration was determined using a microspectrophotometer. Reverse transcription was performed according to the kit protocol, and 1000 ng of RNA was used for reverse transcription and quantitative PCR. qPCR was used to detect the expression level of the COL1A1 gene, which is associated with collagen synthesis.

[0099] The test results are as follows Figure 2 shown.

[0100] from Figure 2 As can be seen, compared with the control group, the plant extracts used in groups S1 to S4, including Kava kava root extract, Centella asiatica extract, Gentiana lutea root extract, White Vitex tiliaceae extract, and Agave equisetum stem extract, significantly increased the expression level of the COL1A1 gene, which is associated with collagen synthesis. This indicates that the plant extracts used in the present invention can promote collagen synthesis and have anti-aging effects.

[0101] From groups D1 to D4, it can be seen that groups D3 to D4 only used two extracts, resulting in lower COL1A1 gene expression levels. Compared to groups D3 and D4, group D2 contained three extracts, and group D1 contained four extracts, and COL1A1 gene expression levels in groups D1 and D2 were significantly increased. This suggests that there is a synergistic effect between Kava Kava root extract, Centella asiatica extract, Gentiana dahurica root extract, White Rose extract, and Agave equisetum stem extract. Only when these five extracts are present together can the synergistic effect between the components be significantly enhanced.

[0102] From groups S1 to S4 and groups D5 to D7, it can be seen that when Kava kava root extract, Centella asiatica extract, Gentiana scabra root extract, White osmanthus fragrans extract, and Agave equisetum stem extract are compounded in appropriate proportions, the upregulation effect on COL1A1 expression levels is more obvious.

[0103] Effect Test Example 2

[0104] Hyaluronic acid is synthesized by fibroblasts in the dermis. A high concentration of hyaluronic acid in the skin can slow the degeneration of collagen and elastin, slowing their conversion from soluble to insoluble proteins. This improves the intercellular colloidal solution environment, thereby smoothing out wrinkles caused by the spaces between cells. However, ultraviolet (UV) radiation triggers the production of hyaluronidase in the skin, which degrades hyaluronic acid and causes photoaging. Hyaluronidase is a type of glycosidase widely found in nature that degrades hyaluronic acid (HA) by acting on β-1,3 or β-1,4 glycosidic bonds.

[0105] The present invention uses the compositions obtained in the above examples and comparative examples as samples, and uses the ELISA method to detect the inhibitory effect of each sample on hyaluronidase; a hyaluronidase ELISA kit is prepared, and the following test is performed according to the instructions of the kit:

[0106] (1) The sample was mixed with PBS buffer solution (pH 7) at a ratio of 1:29 to prepare the test sample. After equilibration at room temperature for 20 minutes, the required strips were removed from the aluminum foil bag.

[0107] (2) Set up blank wells, control wells, and sample wells. Add 50 μL of hyaluronidase (concentration of 1000 U / L) to the control wells and sample wells respectively. Do not add enzyme to the blank wells. Incubate at 37°C for 10 minutes. Then add the test sample to the sample wells respectively. Do not add enzyme to the blank wells and control wells. Incubate for 10 minutes. Wash the plate 5 times.

[0108] (3) Except for the blank wells, add 100 μL of horseradish peroxidase-labeled detection antibody to the control wells and sample wells, seal each well with a sealing film, and incubate at 37°C in a constant temperature box for 60 min;

[0109] (4) Discard the liquid, pat dry with absorbent paper, and wash the plate three times;

[0110] (5) Add 50 μL of substrate A and B to each well and incubate at 37°C in the dark for 15 min;

[0111] (6) Add 50 μL of stop solution to each well. Within 15 minutes, measure the OD value of each well at a wavelength of 450 nm. Calculate the inhibition rate according to the following formula: Inhibition rate (%) = (OD 空白孔 +OD 对照孔 -OD 样品孔 ) / (OD 空白孔 +OD 对照孔 )×100%.

[0112] The test results are as follows Figure 3 shown.

[0113] from Figure 3It can be seen that the plant extracts used in the embodiments of the present invention contain Kava kava root extract, Centella asiatica extract, Gentiana scabra root extract, Bletilla striata extract, and Agave equisetum stem extract, which have a certain inhibitory effect on hyaluronidase and have good soothing effect.

[0114] Compared with Example 1, the plant extracts used in Comparative Examples 3-4 contained only two extracts, and the inhibition rate on hyaluronidase was significantly reduced. Compared with Comparative Examples 3-4, the plant extracts in Comparative Example 1 contained four extracts, and the plant extracts in Comparative Example 2 contained three extracts. The inhibition rate on hyaluronidase in Comparative Examples 1-2 was significantly higher than that in Comparative Examples 3-4. This indicates that there is a synergistic effect between the Piper methysticum root extract, Centella asiatica extract, Gentiana scabra root extract, White Rose extract, and Agave equisetum stem extract. Only when the above five extracts are present together can the inhibitory effect of the composition on hyaluronidase be significantly enhanced.

[0115] It can be seen from Example 1, Examples 5 to 7 and Comparative Examples 5 to 7 that when the Piper methysticum root extract, Centella asiatica extract, Gentiana scabra root extract, Psoralea corylifolia extract and Agave equisetum stem extract are compounded in appropriate proportions, the inhibitory effect of the composition on hyaluronidase is more obvious.

[0116] Effect Test Example 3

[0117] The compositions obtained in Examples 1 to 4 and Comparative Examples 1 and 7 were tested as follows:

[0118] 1. 140 volunteers aged 20 to 45 with capsaicin-positive sensitive skin were randomly divided into seven groups. After daily cleansing, the volunteers were massaged with the composition on their entire face. After 28 days, dermatologists assessed the skin condition of the volunteers according to the "Safety Technical Specifications for Cosmetics - 2015 Edition" for adverse skin reactions. The number of participants experiencing irritation reactions (such as erythema, papules, blisters, and edema) was counted.

[0119] 2. 140 volunteers aged between 20 and 45 were selected and randomly divided into 7 groups. After cleansing every day, they were massaged with the composition on their entire face for 2 consecutive months (60 days).

[0120] (1) The skin moisture content of the volunteers was measured using a skin moisture meter (CM825), and the increase in skin moisture content was calculated according to the following formula: increase in skin moisture content = skin moisture content after use - skin moisture content before use.

[0121] (2) The skin elasticity of the volunteers was measured using a skin elasticity meter, and the skin elasticity improvement rate was calculated according to the following formula: Skin elasticity improvement rate = (skin elasticity parameter after use - skin elasticity parameter before use) * 100%.

[0122] The test results are shown in Table 2.

[0123] Table 2

[0124] Group Number of stimulus responses Increase in skin hydration Skin elasticity improvement rate Example 1 0 7.4% 16.2% Example 2 0 6.3% 13.2% Example 3 0 4.1% 9.3% Example 4 2 5.4% 7.6% Comparative Example 1 0 3.1% 4.1% Comparative Example 7 0 3.4% 2.3% Comparative Example 8 5 4.6% 6.8%

[0125] As can be seen from Table 2, compared with Examples 3-4 and Comparative Examples 1 and 7-8, the compositions provided by Examples 1-2 of the present invention are mild and non-irritating, suitable for sensitive skin, and have better moisturizing and repairing anti-aging effects.

[0126] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A skin-revitalizing, nourishing and soothing composition, characterized in that: The invention comprises the following components in percentage by mass: 0.5-5% plant extract, 5-6.9% moisturizer, 0.01-0.05% chelating agent, 0.05-0.25% thickener, 0.01-0.1% heparin sodium, 16-28% emollient, 0.1-0.5% antioxidant, 4.5-7% emulsifier, 1.1-1.3% pH regulator, 0.1-0.5% preservative, and the balance water. The plant extract is composed of agave stem extract, kava root extract, centella asiatica extract, gentian root extract, and white violet extract. The mass ratio of the kava root extract, centella asiatica extract, gentian root extract, white violet extract, and agave stem extract is (1-4): (0.5-2): (3-6): (0.5-2):

10. In the skin-revitalizing, nourishing and soothing composition, the mass percentages of the emollient components are as follows: sea buckthorn oil 0.05-0.3%, jojoba oil 0.5-3%, cyclopentasiloxane 0.4-2.4%, cyclohexasiloxane 0.1-0.6%, caprylic / capric triglyceride 0.5-2%, shea butter 0.1-0.5%, isopropyl myristate 3-10%, ethylhexyl palmitate 3-10%, hydrogenated polyisobutene 1-4%, and macadamia seed oil 0.05-0.3%; In the skin-revitalizing, moisturizing and soothing composition, the mass percentages of the various components of the emulsifier are as follows: 1-3% cetearyl alcohol, 1-4% first mixed emulsifier, and 0.5-2% second mixed emulsifier, wherein the first mixed emulsifier is compounded with cetearyl alcohol-80 and cetearyl glucoside-20 in a mass ratio of (75-85):(15-25), and the second mixed emulsifier is compounded with glyceryl stearate-65 and PEG-100 stearate-35 in a mass ratio of (50-75):(25-50).

2. The skin-revitalizing, nourishing and soothing composition according to claim 1, wherein: The mass ratio of the Piper methysticum root extract, Centella asiatica extract, Gentiana scabra root extract, Psoralea corylifolia extract and Agave equisetum stem extract is (2-3): (1-1.5): (4-5): (1-1.5):

10.

3. The skin-revitalizing, nourishing and soothing composition according to claim 1, wherein: The invention comprises the following components in percentage by mass: 2-4% plant extract, 6-6.5% moisturizing agent, 0.02-0.04% chelating agent, 0.16-0.2% thickener, 0.02-0.05% heparin sodium, 20.4-21.8% emollient, 0.12-0.3% antioxidant, 5.1-6.3% emulsifier, 1.1-1.3% pH adjusting agent, 0.1-0.5% preservative and the balance water.

4. The skin-revitalizing, nourishing and soothing composition according to claim 1, wherein: The thickening agent includes carbomer.

5. The skin-revitalizing, nourishing and soothing composition according to claim 1, wherein: The moisturizing agent includes at least one of sodium hyaluronate, betaine, glycerol, glyceryl polyether-26, allantoin, panthenol, trehalose, dipropylene glycol, and butylene glycol.

6. The skin-revitalizing, nourishing and soothing composition according to claim 1, wherein: The pH regulator is a compound of arginine and water, wherein the mass percentage of arginine in the composition is 0.1-0.3%.

7. The skin-revitalizing, nourishing and soothing composition according to claim 1, wherein: The antioxidant includes tocopheryl acetate.

8. A method for preparing the skin-revitalizing, nourishing and soothing composition according to any one of claims 1 to 7, characterized in that: The steps include: S1. Mix water, humectant, chelating agent, thickener, and heparin sodium, heat to 82-85°C, and stir until completely dissolved to obtain phase P1. S2, mixing the emulsifier, emollient and antioxidant and heating them to 78-82°C to dissolve them completely to obtain phase P2; S3. Add phase P2 to phase P1 under stirring, homogenize for 5-8 minutes until completely dissolved, keep warm for 15-20 minutes, and defoam. S4, cooling the mixture after defoaming in step S3 to 64-66°C, adding pH regulators in sequence, stirring evenly, and then continuing to cool to a temperature not higher than 45°C, adding plant extracts and preservatives in sequence, and stirring evenly; S5. Adjust the temperature of the mixture obtained in step S4 to 38-40° C., filter, discharge, and allow to stand to obtain the skin-revitalizing, moisturizing, and soothing composition.

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