Multi-target composition for promoting water locking and moisturizing of skin as well as preparation method and medical beauty application of multi-target composition

Abstract

The invention relates to the technical field of medicine and beauty, in particular to a multi-target composition for promoting water locking and moisturizing of skin as well as a preparation method and medical beauty application of the multi-target composition. The composition is prepared from hexapeptide-9, tripeptide-1, carnosine, ergothioneine, a piper nigrum extract, an oat extract, a folium mori extract, Ectoin, glycosylglycerol, sodium hyaluronate, allantoin, butanediol and water. The composition is prepared through specific matching of different raw materials, generation of silk protein, tight junction protein and endogenous natural moisturizing factors can be promoted, and the epidermal hydration force and the skin water locking and moisturizing effect are enhanced; the expression of aquaporin AQP3 is promoted, and the water transfer (transferring to a water-deficient part) in the skin and the skin repairing capability are enhanced. When the composition is applied to skin medicine, skin barrier repair can be accelerated; when being applied to skin beautification, the composition realizes endogenous water locking and moisturizing from multiple target points, improves the skin barrier, and enables the skin to be moisturized, moistened, plump and elastic for a long time.

Description

Technical Field

[0001] The present invention relates to the field of medicine and cosmetic technology, and in particular to a composition for promoting skin moisture retention at multiple targets, a preparation method thereof, and medical and cosmetic applications. Background Art

[0002] The skin is the largest organ in the human body and the first barrier between the human body and the external environment. Maintaining the moisture balance of the skin is essential for the normal function of the skin. Lack of moisture will lead to a decrease in the skin's natural moisturizing factor, making the skin dry, rough and lose elasticity. Sufficient water replenishment can help maintain the moisture of the skin, improve the skin texture, and make it softer and smoother.

[0003] Traditional quick moisturizing solutions usually exogenously replenish the moisture of the stratum corneum or reduce transepidermal water loss. The raw materials for quickly replenishing the function of the stratum corneum include hygroscopic agents, hydrating agents and sealing agents. These substances can quickly make up for the skin's ability to capture and lock water. For example, the most commonly used glycerin has a good hygroscopic capacity. Hyaluronic acid and its derivatives can provide better skin affinity or good film-forming properties while providing moisturizing functions to avoid excessive water loss. Methods for quickly replenishing stratum corneum components include directly adding natural moisturizing factor components (such as sodium pyrrolidone carboxylate) or adding intercellular lipid components (such as ceramide). Some plant-derived water-soluble ceramides can quickly improve the hydration level of the skin and are easy to use. In addition, adding raw materials with similar lipid structures is also a quick and effective moisturizing method. These raw materials can directly make up for the missing parts of the lipid barrier, improve the lipid barrier function, form similar lipid structures in the formula, and also maintain lipid-like structures on the skin.

[0004] The solution of exogenous supplementation of the stratum corneum barrier is fast, but it cannot fundamentally and long-term improve the stratum corneum barrier. For aging skin, when the stratum corneum barrier is damaged, the barrier repair process is always in a lagging state, which will directly cause the stratum corneum barrier to become increasingly weak. If we pay attention to the formation process of the stratum corneum barrier and the moisturizing-related components at a deeper level of the skin, and target these targets to make it express healthily, we can truly improve the skin barrier and achieve long-term moisturizing.

[0005] Promoting the expression of keratin can enhance the internal strength of keratinocytes, increase the expression of proteins (promoting loricrin, endothelial protein, small molecule proline-rich protein) and enzymes (transglutaminase) related to the formation of cornified envelope, and enhance the toughness and stability of the outside of keratinocytes.

[0006] The natural moisturizing factor in the stratum corneum cells is one of the key parts of the stratum corneum for skin moisturizing. It is derived from filaggrin. First, the precursor of filaggrin, profilaggrin, is synthesized in the granular layer cells and aggregated together to form many transparent protein particles. In the process of cells transforming from the granular layer to the stratum corneum, profilaggrin is dephosphorylated and hydrolyzed into filaggrin. Filaggrin and keratin fibers are tightly bound and entangled together to form a complex that fills the newly formed stratum corneum cells and provides strength for the stratum corneum cells. In the process of the stratum corneum migrating to the surface of the skin, keratin is strongly cross-linked with disulfide bonds. After the stratum corneum cell structure is stabilized, filaggrin is unbound from keratin. This process is mostly affected by the dry environment of the outside world. When the water activity or relative humidity is lower than 95%, the protease in the cells begins to activate and gradually degrades the filaggrin completely into amino acids. This may be caused by the increase in ion concentration caused by the decrease in water content in the cells. The collection of these amino acids is called the natural moisturizing factor. Some amino acids will undergo further changes, such as glutamic acid forming pyrrolidone carboxylic acid, and histidine forming urocanic acid under the action of histidase. The ultraviolet absorption function of urocanic acid may provide natural sun protection for the stratum corneum. These weakly acidic products also make the stratum corneum weakly acidic, becoming an important component of the stratum corneum's anti-microbial barrier. In the process of filaggrin conversion into natural moisturizing factor, caspase-14 plays a decisive role in its normal degradation. When keratinocytes slowly move close to the skin surface, the hydration level of keratinocytes decreases to a certain extent, and Caspase-14 will mark the sites where filaggrin needs to be cut, and then directly or indirectly degrade filaggrin into smaller fragments, generating free amino acids, and finally forming a natural moisturizing factor.

[0007] A complete moisturizing program not only includes exogenous supplementation, but also requires targeted endogenous enhancement. Exogenous supplementation of the stratum corneum barrier ingredients achieves rapid moisturizing effects, while stimulating the skin's self-repair ability from the endogenous source, strengthening the integrity and moisturizing of the barrier, so that the skin can achieve hydration, moisturizing and youthful performance. Summary of the invention

[0008] In view of the deficiencies in the above-mentioned prior art, the purpose of the present invention is to provide a multi-target composition for promoting skin water lock and moisturizing, and its preparation method and medical beauty application. The composition is compounded with a specific proportion of hexapeptide-9, tripeptide-1, carnosine, ergothioneine, kava pepper extract, oat extract, mulberry leaf extract, ectoine, glycerol glucoside, sodium hyaluronate, allantoin and water, from the aspect of improving skin hydration, especially by promoting filamentous protein, tight junction protein, and promoting the production of endogenous natural moisturizing factor, enhancing the hydration capacity of the epidermis, thereby improving the skin's water lock and moisturizing ability, by promoting the expression of aquaporin AQP3, strengthening the water transport function in the skin, accelerating the transfer of water to the dehydrated parts, and at the same time providing excellent soothing and anti-inflammatory effects, can efficiently strengthen the barrier function, and is beneficial to skin damage repair. The composition can be used in dermatology to accelerate the repair of the skin barrier; it can be used in skin beauty to achieve endogenous water lock and moisturizing from multiple targets, improve the skin barrier, make the skin lastingly moisturized, nourished, plump and elastic, and can effectively inhibit the stimulation of exogenous or endogenous inflammatory factors to the skin, imparting a highly effective soothing and anti-inflammatory effect.

[0009] On the one hand, this patent discloses a multi-target composition for promoting skin moisture retention, characterized in that it includes hexapeptide-9, tripeptide-1, carnosine, ergothioneine, kava extract, oat extract, mulberry leaf extract, ectoine, glyceryl glucoside, sodium hyaluronate, allantoin, butylene glycol, and water.

[0010] Furthermore, in parts by weight, the composition includes: 0.00001-0.2 parts of hexapeptide-9, 0.00001-0.2 parts of tripeptide-10.00001-0.2 parts of carnosine, 0.00001-0.2 parts of ergothioneine, 0.00001-1 parts of kava extract, 0.00001-2 parts of oat extract, 0.00001-2 parts of mulberry leaf extract, 0.001-2 parts of ectoine, 0.0001-3.5 parts of glycerol glucoside, 0.001-1 parts of sodium hyaluronate, 0.0001-0.5 parts of allantoin, 0.5-3 parts of butylene glycol and 5-150 parts of water.

[0011] Furthermore, the amount of hexapeptide-9 in the composition can be any value and composition range of 0.0001, 0.001, 0.01, 0.05, 0.1, and 0.2 parts.

[0012] Furthermore, the amount of tripeptide-1 in the composition can be any value and composition range of 0.0001, 0.001, 0.01, 0.05, 0.1, and 0.2 parts.

[0013] Furthermore, the amount of carnosine in the composition can also be any value and composition range of 0.0001, 0.001, 0.01, 0.05, 0.1, and 0.2 parts.

[0014] Further, the amount of ergothioneine in the composition can also be any value and composition range of 0.0001, 0.001, 0.01, 0.05, 0.1, 0.2, and 0.5 parts.

[0015] Furthermore, the amount of Kava extract in the composition can be any value and composition range of 0.001, 0.01, 0.1, 0.5, 0.8, 1, and 1.5 parts.

[0016] Furthermore, the kava extract needs to use its root as a raw material and use an ethanol aqueous solution with a concentration of 25-40wt% for extraction. The extract obtained in this way has a better effect.

[0017] Furthermore, the preparation method of the kava extract comprises: crushing the root of the kava, adding 10-30 times the mass of a 25-40wt% ethanol aqueous solution, heating and stirring at 40-65°C for 4-10 hours, centrifuging, taking the supernatant and freeze-drying to obtain the kava extract, which is more suitable for use in the composition of the present invention.

[0018] Furthermore, the amount of oat extract in the composition can be any value and composition range of 0.001, 0.01, 0.1, 0.5, 0.8, 1, and 1.5 parts.

[0019] Furthermore, the oat extract needs to be extracted using oat grains as raw materials using hot water (eg, 55-70° C.).

[0020] Furthermore, the preparation method of the oat extract includes: crushing oat grains, adding 15-40 times the mass of water, heating and stirring at 55-70° C. for 5-10 hours, centrifuging, taking the supernatant and freeze-drying to obtain the oat extract.

[0021] Furthermore, the amount of mulberry leaf extract in the composition can also be any value and composition range of 0.001, 0.01, 0.1, 0.5, 0.8, 1, and 1.5 parts.

[0022] Furthermore, the mulberry leaf extract needs to use its leaves as raw materials and use an ethanol aqueous solution with a concentration of 10-25wt% for extraction. The extract obtained in this way has better effect and is more suitable for the composition system of the present invention.

[0023] Furthermore, the preparation method of mulberry leaf extract comprises: crushing fresh mulberry leaves, adding 10-50 times the mass of 10-25wt% ethanol aqueous solution, heating and stirring at 35-55°C for 6-12 hours, centrifuging, taking the supernatant and freeze-drying to obtain mulberry leaf extract.

[0024] Furthermore, in order to promote the soothing and anti-inflammatory functions, the mass ratio of kava extract to mulberry leaf extract is 1.5-3:1, and can also be 1.6:1, 1.7:1, 1.8:1, 2:1, 2.2:1, 2.5:1, 2.8:1, 3:1, etc.

[0025] Furthermore, the amount of ectoine in the composition can also be any value and composition range of 0.01, 0.05, 0.1, 0.5, 1, 1.2, and 1.5 parts.

[0026] Furthermore, the amount of glycerol glucoside in the composition can be any value and composition range of 0.01, 0.05, 0.1, 0.5, 1, 1.2, 1.5, 2, 3, and 3.5 parts.

[0027] Furthermore, the amount of sodium hyaluronate in the composition can be any value and composition range of 0.01, 0.05, 0.1, 0.2, 0.5, and 1 part.

[0028] Furthermore, the amount of allantoin in the composition can be any value and composition range of 0.001, 0.01, 0.05, 0.1, 0.2, and 0.5 parts.

[0029] Furthermore, the amount of butanediol in the composition can be any value and composition range of 0.5, 0.8, 1, 1.2, 1.5, 2, 2.5, and 3 parts.

[0030] Furthermore, the amount of water in the composition can be any value and range of 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 120, 150 parts.

[0031] Furthermore, in parts by weight, the composition includes: 0.001-0.1 parts of hexapeptide 9, 0.001-0.1 parts of tripeptide 10.001-0.1 parts of carnosine, 0.001-0.1 parts of ergothioneine, 0.001-0.5 parts of kava extract, 0.1-1.8 parts of oat extract, 0.1-1.8 parts of mulberry leaf extract, 0.1-1.8 parts of ectoine, 0.1-2 parts of glycerol glucoside, 0.1-0.5 parts of sodium hyaluronate, 0.001-0.2 parts of allantoin, 0.8-2.5 parts of butylene glycol and 20-100 parts of water.

[0032] Furthermore, based on the effective promotion of moisturizing and skin repair, the weight ratio of oat extract and ectoine is 1:0.5-1, and can also be 1:0.6, 1:0.7, 1:0.8, 1:0.9, etc.

[0033] Another aspect of the present invention discloses a method for preparing the above-mentioned composition, comprising:

[0034] Step 1: Prepare the ingredients;

[0035] Step 2: Add water into a container, heat it, then add butylene glycol and stir, then add glyceryl glucoside, sodium hyaluronate and allantoin, stir to obtain mixture 1;

[0036] Step 3: Cool the mixture, add hexapeptide-9, tripeptide-1, carnosine, ergothioneine, kava extract, oat extract, mulberry leaf extract, and ectoine, stir, stand for aging, sterilize, and fill to obtain the composition.

[0037] Furthermore, step 2 is heating to 40-55°C, and step 3 is cooling to 30-35°C.

[0038] Furthermore, in step 3, the static aging temperature can be room temperature.

[0039] On the other hand, this patent discloses the use of the above composition in the preparation of medical beauty preparations.

[0040] On the other hand, this patent discloses the use of the above composition in the preparation of moisturizing cosmetics.

[0041] On the other hand, the patent discloses the use of the above composition in the preparation of skin soothing cosmetics.

[0042] Compared with the prior art, the present invention is progressive in that:

[0043] The present invention uses hexapeptide-9, tripeptide-1, carnosine, ergothioneine, kava pepper extract, oat extract, mulberry leaf extract, ectoine, glycerol glucoside, sodium hyaluronate, allantoin, butanediol and water to prepare a composition. The present invention prepares a composition by specific matching of different raw materials, which can promote the generation of filaggrin, tight junction protein and endogenous natural moisturizing factor, enhance epidermal hydration and skin water-locking and moisturizing effect; promote the expression of aquaporin AQP3, strengthen the water transport in the skin (transfer to the water-deficient parts) and repair the skin ability. The composition is applied in dermatology to accelerate the repair of the skin barrier; applied in skin beauty, it can achieve endogenous water-locking and moisturizing from multiple targets, improve the skin barrier, and make the skin lastingly moisturized, nourished, plump and elastic.

[0044] The product prepared by the present invention can effectively increase the water content of the stratum corneum of the skin and reduce the water loss rate of the skin through the epidermis; it has excellent moisturizing and water-locking effects and has good skin barrier repair ability.

[0045] The combination of oat extract and ectoine in the product prepared by the present invention can synergistically enhance the moisturizing and water-locking and skin barrier repairing capabilities of the composition. The effect of oat extract obtained by other extraction methods other than the present invention is not as good as that of the present method, and cannot give the product of the present invention excellent effects.

[0046] The product prepared by the present invention can effectively inhibit the expression of inflammatory factors IL-6 and TNF-α, has good soothing and anti-inflammatory effects, and can inhibit the damage of the skin barrier and the decrease of moisturizing and water-locking ability caused by inflammatory factors.

[0047] The present invention adopts the combination of kava extract and mulberry leaf extract to produce a mutually reinforcing relationship, which can effectively increase the anti-inflammatory and soothing effect of the product. When the mass ratio of kava extract to mulberry leaf extract is 1.5-3:1, the anti-inflammatory effect of the product is better.

[0048] The invention adopts 25-40wt% ethanol aqueous solution to extract Kava pepper and adopts 10-25wt% ethanol aqueous solution to extract mulberry leaves, and the obtained extracts have better anti-inflammatory and soothing effects of the products.

[0049] The product prepared by the invention has excellent performance, a simple preparation method and good application prospects. DETAILED DESCRIPTION

[0050] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. The following description of at least one exemplary embodiment is actually only illustrative and is by no means intended to limit the present invention and its application or use. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0051] The raw material information used in the specific implementation of the present invention is as follows:

[0052] Hexapeptide-9: Sichuan Weikeqi Biotechnology Co., Ltd. Carnosine: L-carnosine, product number C106843; Ergothioneine: L-(+)-ergothioneine, product number L134175; Ectoin: product number E292674; Glyceryl Glucoside: product number G768334; Sodium Hyaluronate: product number S774025; Allantoin: product number A101660; Butanediol: 1,3-Butanediol, product number B111016; from Shanghai Aladdin Biochemical Technology Co., Ltd. Tripeptide-1: CAS: 72957-37-0, Shanghai Kepeptide Biotechnology Co., Ltd.

[0053] 1. Preparation of products

[0054] Product 1:

[0055] The preparation method is as follows:

[0056] Step 1: Prepare raw materials according to mass parts; 90.05 parts of hexapeptide, 10.04 parts of tripeptide, 0.03 parts of carnosine, 0.09 parts of ergothioneine, 1.6 parts of kava extract, 1.5 parts of oat extract, 0.9 parts of mulberry leaf extract, 1.2 parts of ectoine, 0.3 parts of glyceryl glucoside, 0.2 parts of sodium hyaluronate, 0.05 parts of allantoin, 1.5 parts of butylene glycol and 65 parts of water; the mass ratio of kava extract to mulberry leaf extract is 2:1; the weight ratio of oat extract to ectoine is 1:0.8.

[0057] The preparation method of the kava extract is as follows: the root of the kava pepper is crushed, 15 times the mass of a 30wt% ethanol aqueous solution is added, heated at 60°C and stirred at 50rpm for 8 hours, centrifuged at 1000rpm for 0.4 hours, and the supernatant is freeze-dried at -45°C to a water content of 2.01wt%, to obtain the kava extract.

[0058] The preparation method of oat extract is as follows: crush oat grains, add 30 times the weight of water, heat at 62°C and stir at 55rpm for 9.5 hours, centrifuge at 1000rpm for 0.3 hours, take the supernatant and freeze-dry at -45°C to a water content of 1.97wt%, to obtain oat extract.

[0059] The preparation method of mulberry leaf extract is as follows: crush fresh mulberry leaves, add 20 times the mass of 20wt% ethanol aqueous solution, heat at 48°C and stir at 60rpm for 10 hours, centrifuge at 900rpm for 0.3 hours, take the supernatant and freeze-dry at -45°C to a water content of 1.85wt%, to obtain mulberry leaf extract.

[0060] Step 2: Add water into a container, heat to 50°C, then add butanediol, stir at 60rpm for 0.2 hours, then add glyceryl glucoside, sodium hyaluronate and allantoin, keep stirring at the same speed for 0.3 hours, and obtain mixture 1;

[0061] Step 3: The mixture is cooled to 30°C, and hexapeptide-9, tripeptide-1, carnosine, ergothioneine, kava extract, oat extract, mulberry leaf extract, and ectoine are added. The stirring is continued for 0.4 hours while maintaining the speed. The mixture is allowed to stand for 1 hour at room temperature, sterilized, and filled to obtain product 1.

[0062] Product 2:

[0063] The preparation method is as follows:

[0064] Step 1: Prepare raw materials according to mass parts; 90.04 parts of hexapeptide, 10.03 parts of tripeptide, 0.06 parts of carnosine, 0.08 parts of ergothioneine, 1.85 parts of kava extract, 1.8 parts of oat extract, 0.65 parts of mulberry leaf extract, 0.9 parts of ectoine, 0.2 parts of glyceryl glucoside, 0.22 parts of sodium hyaluronate, 0.05 parts of allantoin, 1.6 parts of butylene glycol and 65 parts of water; the mass ratio of kava extract to mulberry leaf extract is 2.7:1; the weight ratio of oat extract to ectoine is 1:0.5.

[0065] The preparation method of the kava extract is as follows: the root of the kava pepper is crushed, 20 times the mass of a 35wt% ethanol aqueous solution is added, heated at 60°C and stirred at 50rpm for 7 hours, centrifuged at 1000rpm for 0.4 hours, and the supernatant is freeze-dried at -45°C to a water content of 2.09wt%, thereby obtaining the kava extract.

[0066] The preparation method of oat extract is as follows: crush oat grains, add 40 times the mass of water, heat at 60°C and stir at 60rpm for 10 hours, centrifuge at 1200rpm for 0.3 hours, take the supernatant and freeze-dry at -45°C to a water content of 1.91wt%, to obtain oat extract.

[0067] The preparation method of mulberry leaf extract is as follows: crush fresh mulberry leaves, add 30 times the mass of 10wt% ethanol aqueous solution, heat at 50°C and stir at 60rpm for 8.5 hours, centrifuge at 1000rpm for 0.3 hours, take the supernatant and freeze-dry at -45°C to a water content of 1.93wt%, to obtain mulberry leaf extract.

[0068] Step 2: Add water into a container, heat to 40°C, then add butanediol, stir at 60rpm for 0.2 hours, then add glyceryl glucoside, sodium hyaluronate and allantoin, keep stirring at the same speed for 0.4 hours, and obtain mixture 1;

[0069] Step 3: The mixture is cooled to 32°C, and hexapeptide-9, tripeptide-1, carnosine, ergothioneine, kava extract, oat extract, mulberry leaf extract, and ectoine are added. The stirring is continued for 0.5 hours at the same speed, and the mixture is allowed to stand at room temperature for 1.2 hours, sterilized, and filled to obtain product 2.

[0070] Product 3:

[0071] The only difference from product 1 is: Step 1: prepare raw materials according to mass parts; 90.05 parts of hexapeptide, 10.04 parts of tripeptide, 0.03 parts of carnosine, 0.09 parts of ergothioneine, 1.6 parts of kava extract, 0.9 parts of mulberry leaf extract, 2.7 parts of ectoine, 0.3 parts of glyceryl glucoside, 0.2 parts of sodium hyaluronate, 0.05 parts of allantoin, 1.5 parts of butylene glycol and 65 parts of water.

[0072] Product 4:

[0073] The only difference from product 1 is: Step 1: prepare raw materials according to mass parts; 90.05 parts of hexapeptide, 10.04 parts of tripeptide, 0.03 parts of carnosine, 0.09 parts of ergothioneine, 1.6 parts of kava extract, 2.7 parts of oat extract, 0.9 parts of mulberry leaf extract, 0.3 parts of glyceryl glucoside, 0.2 parts of sodium hyaluronate, 0.05 parts of allantoin, 1.5 parts of butylene glycol and 65 parts of water.

[0074] Product 5:

[0075] The only difference from product 1 is that the preparation method of oat extract is as follows: crush oat grains, add 30 times the mass of 65wt% ethanol aqueous solution, heat at 62°C and stir at 55rpm for 9.5 hours, centrifuge at 1200rpm for 0.3 hour, take the supernatant and freeze-dry it at -45°C to a water content of 1.97wt%, to obtain oat extract.

[0076] Product 6:

[0077] The only difference from product 1 is: Step 1: prepare raw materials according to mass parts; 90.05 parts of hexapeptide, 10.04 parts of tripeptide, 0.03 parts of carnosine, 0.09 parts of ergothioneine, 2.5 parts of kava extract, 1.5 parts of oat extract, 1.2 parts of ectoine, 0.3 parts of glyceryl glucoside, 0.2 parts of sodium hyaluronate, 0.05 parts of allantoin, 1.5 parts of butylene glycol and 65 parts of water.

[0078] Product 7:

[0079] The only difference from product 1 is: Step 1: prepare raw materials according to mass parts; 90.05 parts of hexapeptide, 10.04 parts of tripeptide, 0.03 parts of carnosine, 0.09 parts of ergothioneine, 1.5 parts of oat extract, 2.5 parts of mulberry leaf extract, 1.2 parts of ectoine, 0.3 parts of glyceryl glucoside, 0.2 parts of sodium hyaluronate, 0.05 parts of allantoin, 1.5 parts of butylene glycol and 65 parts of water.

[0080] Product 8:

[0081] The only difference from product 1 is: Step 1: prepare raw materials according to mass parts; 90.05 parts of hexapeptide, 10.04 parts of tripeptide, 0.03 parts of carnosine, 0.09 parts of ergothioneine, 0.4 parts of kava extract, 1.5 parts of oat extract, 2.1 parts of mulberry leaf extract, 1.2 parts of ectoine, 0.3 parts of glyceryl glucoside, 0.2 parts of sodium hyaluronate, 0.05 parts of allantoin, 1.5 parts of butylene glycol and 65 parts of water; the mass ratio of kava extract to mulberry leaf extract is 0.19:1.

[0082] Product 9:

[0083] The only difference from product 1 is: Step 1: prepare raw materials according to mass parts; 90.05 parts of hexapeptide, 10.04 parts of tripeptide, 0.03 parts of carnosine, 0.09 parts of ergothioneine, 2.2 parts of kava extract, 1.5 parts of oat extract, 0.3 parts of mulberry leaf extract, 1.2 parts of ectoine, 0.3 parts of glyceryl glucoside, 0.2 parts of sodium hyaluronate, 0.05 parts of allantoin, 1.5 parts of butylene glycol and 65 parts of water; the mass ratio of kava extract to mulberry leaf extract is 7.3:1.

[0084] Product 10:

[0085] The only difference from product 1 is that the preparation method of kava extract is as follows: the root of kava is crushed, 15 times the mass of ethanol aqueous solution with a concentration of 70wt% is added, heated at 60°C and stirred at 50rpm for 8 hours, centrifuged at 1000rpm for 0.4 hours, and the supernatant is freeze-dried at -45°C to a water content of 2.01wt%, thereby obtaining kava extract. The preparation method of mulberry leaf extract is as follows: fresh mulberry leaves are crushed, 20 times the mass of ethanol aqueous solution with a concentration of 80wt% is added, heated at 48°C and stirred at 60rpm for 10 hours, centrifuged at 900rpm for 0.3 hours, and the supernatant is freeze-dried at -45°C to a water content of 1.85wt%, thereby obtaining mulberry leaf extract.

[0086] 2. Product Testing

[0087] 1. Transepidermal water loss rate and water content of the stratum corneum

[0088] Transepidermal water loss (TEWL) is an important parameter that reflects the water loss and barrier function of the skin. The lower the measured value, the better the barrier function and repair ability of the skin, and the better the water-locking effect. The present invention uses Vapometer, SWL5201 to test.

[0089] The larger the water content of the stratum corneum, the higher the water content of the stratum corneum, and the better the moisturizing effect of the product. The present invention adopts Cormeometer, CM825 test to obtain.

[0090] The test methods for both are as follows: 40 female volunteers aged 35-55 with healthy skin were selected and randomly divided into 5 groups, with 8 people in each group. Before using the above products, volunteers in each group washed their cheeks and wiped them with a paper towel, and then tested the TEWL and stratum corneum water content of the volunteers' cheeks, and recorded the results. Then the volunteers in each group used the above products 1-5 for testing, and the method of use was: use the corresponding products of each group once at 9:00 and 18:00 every day, and apply 1.5g of the product evenly on the face each time, and use it continuously for 2 months; 3 hours after the last use, test the TEWL and stratum corneum water content of the cheeks of various volunteers again, and record the results. The transepidermal water loss rate (TEWL) of the skin at the beginning and end of the experiment of each group, as well as the reduction and reduction rate after use compared with before use, and the stratum corneum water content of the skin at the beginning and end of the experiment of each group, as well as the increase and increase rate after use compared with before use, are calculated. The results are shown in Tables 1 and 2.

[0091] Table 1: Transepidermal water loss rate TEWL, increase amount and increase rate before and after use

[0092]

[0093] Table 2: A value, increase and increase rate of skin stratum corneum water content before and after use

[0094]

[0095] Combined with the test results in Tables 1-2, it can be seen that the product prepared by the present invention can effectively increase the water content of the stratum corneum of the skin and reduce the water loss rate of the skin through the epidermis; it can be seen that the product of the present invention has an excellent moisturizing and water-locking effect, and has a good skin barrier repair ability.

[0096] Combined with the test results of products 1 and 3-4 in Table 1-2, it can be seen that the effect of product 1 obtained by using oat extract and ectoine is better than that of product 3-4 which keeps the same amount of both as product 1 and uses only one of them. It can be seen that the combination of oat extract and ectoine in the product prepared by the present invention can synergistically enhance the moisturizing and water-locking and skin barrier repair capabilities of the composition. According to the test results of products 1 and 5, the effect of oat extracts obtained by other extraction methods is not as good as this method, and cannot give the product of the present invention excellent effects.

[0097] 2. Soothing and anti-inflammatory

[0098] The test inhibits the release of in vitro cellular inflammatory factors to verify the soothing and anti-inflammatory effects of the product.

[0099] RAW264.7 cells were plated at a density of 1.0 × 10 5ce ll / mL) were inoculated into a 24-well cell culture plate, cultured for 24 hours, and then divided into groups. Except for the blank group, LPS was added to 1 μg / mL in the cells of other groups to establish an inflammatory model; the specific process was: only LPS was added to 1 μg / mL in the model group, and LPS was added to 1 μg / mL in the product 1-2, 6-10 (corresponding to the above products 1-2, 6-10) groups, and the corresponding products of each group were added to 0.015wt%; incubation was continued at 37°C for 24 hours, and then the upper culture fluid was collected, and the expression levels of IL-6 and TNF-α in each group and the reduction values ​​of IL-6 and TNF-α in each group compared with the model group were detected according to the method described in the instructions of the ELISA kit; the test results are shown in Table 3. Among them, the higher the expression levels of IL-6 and TNF-α, the higher the content of inflammatory factors, and the worse the effect of inhibiting inflammation.

[0100] Table 3: IL-6, TNF-α and the reduction values ​​compared with the model group

[0101]

[0102]

[0103] Combined with the test results in Table 3, it can be seen that the product prepared by the present invention can effectively inhibit the expression of inflammatory factors IL-6 and TNF-α, has good soothing and anti-inflammatory effects, and can inhibit skin irritation caused by inflammatory factors.

[0104] Combining products 1 and 6-9 in Table 3, it can be seen that when one of the kava extract and mulberry leaf extract is removed from product 6-7 and the dosage is kept consistent with the total dosage of the two in product 1, the effect of inhibiting inflammatory factors in the obtained product 6-7 is worse than that of product 1. The combination of the two has a mutually reinforcing relationship and can effectively increase the anti-inflammatory and soothing effect of the product on the skin. And when the ratio of kava extract and mulberry leaf extract is changed in products 8-9, the anti-inflammatory effect of the obtained product is relatively poor. When the mass ratio of kava extract and mulberry leaf extract is selected to be 1.5-3:1 in the present invention, the anti-inflammatory effect of the product is better.

[0105] Combined with the test of products 1 and 10, it can be seen that the present invention uses 25-40wt% ethanol aqueous solution to extract kava pepper and uses 10-25wt% ethanol aqueous solution to extract mulberry leaves, and the extracts obtained have better anti-inflammatory and soothing effects of the products.

[0106] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for a person skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to replace some of the technical features therein with equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions claimed to be protected by the present invention.

Claims

1. A composition for promoting skin moisture retention at multiple targets, characterized in that: Includes hexapeptide-9, tripeptide-1, carnosine, ergothioneine, kava extract, oat extract, mulberry leaf extract, ectoine, glyceryl glucoside, sodium hyaluronate, allantoin, butylene glycol, and water.

2. The composition according to claim 1, characterized in that In parts by weight, the invention comprises: 0.00001-0.2 parts of hexapeptide-9, 0.00001-0.2 parts of tripeptide-1, 0.00001-0.2 parts of carnosine, 0.00001-1 parts of ergothioneine, 0.00001-2 parts of kava extract, 0.00001-2 parts of oat extract, 0.00001-2 parts of mulberry leaf extract, 0.001-2 parts of ectoine, 0.0001-3.5 parts of glycerol glucoside, 0.001-1 parts of sodium hyaluronate, 0.0001-0.5 parts of allantoin, 0.5-3 parts of butylene glycol and 5-150 parts of water.

3. The composition according to claim 1, characterized in that In parts by weight, the invention comprises: 0.001-0.1 parts of hexapeptide 9, 0.001-0.1 parts of tripeptide 10.001-0.1 parts of carnosine, 0.001-0.1 parts of ergothioneine, 0.001-0.5 parts of kava extract, 0.1-1.8 parts of oat extract, 0.1-1.8 parts of mulberry leaf extract, 0.1-1.5 parts of ectoine, 0.1-2 parts of glycerol glucoside, 0.1-0.5 parts of sodium hyaluronate, 0.001-0.2 parts of allantoin, 0.8-2.5 parts of butylene glycol and 20-100 parts of water.

4. The composition according to claim 1, characterized in that The mass ratio of kava extract to mulberry leaf extract is 1.5-3:

1.

5. A method for preparing the composition according to any one of claims 1 to 4, characterized in that: include: Step 1: Prepare the ingredients; Step 2: Add water into a container, heat it, then add butylene glycol and stir, then add glyceryl glucoside, sodium hyaluronate and allantoin, stir to obtain mixture 1; Step 3: Cool the mixture, add hexapeptide-9, tripeptide-1, carnosine, ergothioneine, kava extract, oat extract, mulberry leaf extract, and ectoine, stir, stand for aging, sterilize, and fill to obtain the composition.

6. The preparation method according to claim 5, characterized in that: Step 2 is heating to 40-55°C, and step 3 is cooling to 30-35°C.

7. The preparation method according to claim 5, characterized in that: Kava extract is obtained by using the root of Kava as a raw material and extracting it using an ethanol aqueous solution with a concentration of 25-40wt%; And / or, the oat extract is obtained by using oat grains as raw materials and extracting using hot water; And / or, the mulberry leaf extract is obtained by using mulberry leaves as raw materials and extracting with 10-25wt% ethanol aqueous solution.

8. Use of the composition according to any one of claims 1 to 4 in the preparation of medical cosmetic preparations.

9. Use of the composition according to any one of claims 1 to 4 in the preparation of moisturizing cosmetics.

10. Use of the composition according to any one of claims 1 to 4 in preparing skin soothing cosmetics.

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